CN116569836A - Stemona pollen diluent and Stemona artificial pollination method - Google Patents
Stemona pollen diluent and Stemona artificial pollination method Download PDFInfo
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- CN116569836A CN116569836A CN202310779099.9A CN202310779099A CN116569836A CN 116569836 A CN116569836 A CN 116569836A CN 202310779099 A CN202310779099 A CN 202310779099A CN 116569836 A CN116569836 A CN 116569836A
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- Prior art keywords
- pollen
- stemona
- diluent
- radix stemonae
- artificial pollination
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- 239000003085 diluting agent Substances 0.000 title claims abstract description 39
- 230000010152 pollination Effects 0.000 title claims abstract description 38
- 238000000034 method Methods 0.000 title claims abstract description 23
- 241001671204 Stemona Species 0.000 title claims description 38
- 241000196324 Embryophyta Species 0.000 claims description 20
- 239000012895 dilution Substances 0.000 claims description 14
- 238000010790 dilution Methods 0.000 claims description 14
- 239000000725 suspension Substances 0.000 claims description 13
- 241000417534 Stemona sessilifolia Species 0.000 claims description 12
- 229920001213 Polysorbate 20 Polymers 0.000 claims description 10
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims description 10
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 claims description 9
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 claims description 9
- 229960000367 inositol Drugs 0.000 claims description 9
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 claims description 9
- 239000004094 surface-active agent Substances 0.000 claims description 9
- 239000002904 solvent Substances 0.000 claims description 6
- 239000002202 Polyethylene glycol Substances 0.000 claims description 4
- 239000000654 additive Substances 0.000 claims description 4
- 230000000996 additive effect Effects 0.000 claims description 4
- 229920001223 polyethylene glycol Polymers 0.000 claims description 4
- -1 alkyl glycoside Chemical class 0.000 claims description 2
- GVGUFUZHNYFZLC-UHFFFAOYSA-N dodecyl benzenesulfonate;sodium Chemical compound [Na].CCCCCCCCCCCCOS(=O)(=O)C1=CC=CC=C1 GVGUFUZHNYFZLC-UHFFFAOYSA-N 0.000 claims description 2
- 229930182470 glycoside Natural products 0.000 claims description 2
- ZPIRTVJRHUMMOI-UHFFFAOYSA-N octoxybenzene Chemical compound CCCCCCCCOC1=CC=CC=C1 ZPIRTVJRHUMMOI-UHFFFAOYSA-N 0.000 claims description 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 2
- 229920000053 polysorbate 80 Polymers 0.000 claims description 2
- 229940080264 sodium dodecylbenzenesulfonate Drugs 0.000 claims description 2
- 235000013399 edible fruits Nutrition 0.000 abstract description 16
- 238000009395 breeding Methods 0.000 abstract description 6
- 230000001488 breeding effect Effects 0.000 abstract description 6
- 238000007865 diluting Methods 0.000 abstract description 5
- 239000003814 drug Substances 0.000 abstract description 4
- 239000008367 deionised water Substances 0.000 description 5
- 229910021641 deionized water Inorganic materials 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 4
- 206010011224 Cough Diseases 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 241000411860 Stemona tuberosa Species 0.000 description 2
- 230000010154 cross-pollination Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 208000028454 lice infestation Diseases 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000003976 plant breeding Methods 0.000 description 2
- 241000517324 Pediculidae Species 0.000 description 1
- 241000517307 Pediculus humanus Species 0.000 description 1
- 208000008350 Pruritus Vulvae Diseases 0.000 description 1
- 241000244978 Stemonaceae Species 0.000 description 1
- 241000249864 Tussilago Species 0.000 description 1
- 206010056530 Vulvovaginal pruritus Diseases 0.000 description 1
- 208000031971 Yin Deficiency Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 208000013116 chronic cough Diseases 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 206010014881 enterobiasis Diseases 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 230000010153 self-pollination Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- SWGJCIMEBVHMTA-UHFFFAOYSA-K trisodium;6-oxido-4-sulfo-5-[(4-sulfonatonaphthalen-1-yl)diazenyl]naphthalene-2-sulfonate Chemical group [Na+].[Na+].[Na+].C1=CC=C2C(N=NC3=C4C(=CC(=CC4=CC=C3O)S([O-])(=O)=O)S([O-])(=O)=O)=CC=C(S([O-])(=O)=O)C2=C1 SWGJCIMEBVHMTA-UHFFFAOYSA-K 0.000 description 1
- 201000008827 tuberculosis Diseases 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to the technical field of traditional Chinese medicine breeding, in particular to a radix stemonae pollen diluent and an artificial pollination method of radix stemonae. The radix stemonae pollen diluent can be used for diluting pollen collected in the artificial pollination process of radix stemonae so as to improve the success rate of artificial pollination of radix stemonae, thereby solving the problems of low fruit setting rate, few seeds, high artificial pollination difficulty and low success rate of natural propagation of radix stemonae plants.
Description
Technical Field
The invention relates to the technical field of traditional Chinese medicine breeding, in particular to an artificial pollination method of stemona root, application and a preparation method thereof.
Background
The Chinese medicinal material Stemona has three basic plants derived from Stemona sessilifolia (Miq.) Miq. Of Stemona of Stemonaceae, stemona sessilifolia (Bl.) Miq. Or Stemona tuberosa Lour. Stemona root is used for treating chronic cough, tuberculosis and tussilago, and can be used for external application for treating head lice, body lice, enterobiasis and pruritus vulvae. Bai Shao also has the action of moistening lung and relieving cough, and is indicated for cough due to yin deficiency. Stemona tuberosa is mainly distributed in regions of the Yangtze river in south provinces, mainly produced in Guangxi, yunnan, guizhou, hunan and Guangdong; the main products of the Stemona sessilifolia are distributed in Jiangsu, anhui, zhejiang, shandong, henan and the like; the vining Stemona is mainly distributed in Jiangsu, anhui, zhejiang, jiangxi and the like.
With the development of China, the population is increasingly dense, the environment pollution is serious, the incidence rate of a respiratory system is increased, the use level of the stemona is gradually increased, the stemona has weak reproductive capacity in a natural state and can grow slowly, the yield can be formed for many years, the wild is in short supply, the large-area planting condition of the stemona is not reported in the literature at present, and the stemona is planted in a small area and is researched and planted in an experimental mode. Liu Yanqin (Liu Yanqin, cao Min, jin Jiangqun, liu Xu. Influence of different pollination modes on the fruit setting of the stemona root. Modern agriculture technology, 2021 (07): 50-52.) and the like are studied, and the study shows that the natural pollination fruit setting rate of the stemona root is 19.6%, the self-pollination is not fruit setting, the cross-pollination fruit setting rate of the same strain is 20%, the cross-pollination fruit setting rate of different strain is more than 80% and the seed fullness is high, which indicates that the self-compatibility of the stemona root is poor, and the fruit setting rate and the seed quality of the stemona root can be improved by artificial pollination.
Referring to fig. 1A, the structure of the stemona flower is that the stamen of the stemona flower is purple red, and is shorter than or almost equal to the length of the quilt. Referring to fig. 1B, in the stemona flower structure, anthers are arranged on the inner side of stamens, and pistils are wrapped by stamens at the bottom, so that when artificial pollination is performed, red stamens needs to be manually broken off for pollination, the operation difficulty is high, the success rate is low, and the success rate is extremely dependent on the experience of operators. Thus, if the success rate of artificial pollination of plants such as the hundred plants is increased, is the difficulty of breeding the traditional Chinese medicine radix stemonae.
Disclosure of Invention
In order to solve the technical problems, the invention aims to provide the radix stemonae pollen diluent and the radix stemonae artificial pollination method, which can be applied to the field of plant breeding such as hundred parts, so that the problems of low fruit setting rate, few seeds, high artificial pollination difficulty and low success rate of natural plant breeding are solved.
In order to achieve the technical effects, the invention adopts the following technical scheme:
firstly, the invention provides a radix stemonae pollen diluent which comprises a solvent and an additive, wherein the additive at least comprises a surfactant.
Preferably, the solvent is water.
Preferably, the surfactant is any one or more of thiobetaine 12, sodium dodecyl benzene sulfonate, polyethylene glycol, alkyl glycoside, tween 80, tween 20 and polyethylene glycol octyl phenyl ether; tween 20 is preferred.
Preferably, the surfactant is present in the diluent in a ratio of from 0.01wt.% to 5wt.%.
Preferably, the diluent further comprises inositol; preferably, inositol is present in the diluent in a ratio of from 0.01wt.% to 5wt.%, more preferably from 0.01wt.% to 1wt.%.
In addition, the invention also provides application of the stemona pollen diluent in the aspects of the Miq of stemona sessilifolia (Miq.), the Miq of stemona sessilifolia (Bl.) Miq or the breeding of stemona sessilifolia.
In a second aspect, the present invention also provides a method for artificial pollination of Stemona root, comprising the steps of:
s1: collecting pollen;
s2: dispersing the collected pollen by adopting a diluent to form a suspension, wherein the diluent is the radix stemonae pollen diluent according to any one of claims 1 to 3;
s3: the suspension containing pollen is sucked and dropped or sprayed on the flower column head to complete artificial pollination.
Preferably, the mass ratio of the diluent to the pollen is 10-1000:1, preferably 200:1.
Preferably, the pollen collecting process collects at least 2 pollen from different plants of the same plant species; preferably, pollen from at least 10 different plants of the same plant species is collected; more preferably: at least 30 pollen from different plants of the same plant species were collected.
In addition, based on the stemona artificial pollination method provided by the second aspect, the invention also provides an application of the stemona artificial pollination method in the aspect of the cultivation of stemona sessilifolia (Miq.) Miq., stemona sessilifolia (Bl.) Miq. Or stemona sessilifolia.
Compared with the prior art, the invention has the beneficial effects that:
the invention provides a radix stemonae pollen diluent and an artificial pollination method for radix stemonae pollen, which creatively provides the radix stemonae pollen diluent aiming at the problems of low fruit setting rate, few seeds, high artificial pollination difficulty and low success rate of natural plant propagation in the current traditional Chinese medicine breeding process, particularly the radix stemonae medicinal material breeding process, and the surface tension of a solvent is reduced by adding a surfactant into the radix stemonae pollen diluent, so that pollen uniformly or non-uniformly dispersed in the solvent can fully contact with stigma, and the pollination success rate of the pollen is improved.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
Fig. 1 is a schematic diagram of a stemona flower structure provided by the invention;
fig. 2 is a schematic diagram of a method for performing artificial pollination on stemona flowers provided in embodiment 4 of the present invention;
FIG. 3 is a fruit setting chart of stemona flower of example 4 of the present invention after pollination with suspension I;
FIG. 4 is a photograph of seed of Stemona flower of example 4 of the present invention after pollination with suspension I.
Detailed Description
Embodiments of the technical scheme of the present invention will be described in detail below with reference to the accompanying drawings. The following examples are only for more clearly illustrating the technical aspects of the present invention, and thus are merely examples, and are not intended to limit the scope of the present invention.
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments. The specific embodiments of the present invention are listed only as examples of the present invention, and the present invention is not limited to the specific embodiments described below.
Any equivalent modifications and substitutions of the embodiments described below will be apparent to those skilled in the art, and are intended to be within the scope of the present invention. Accordingly, equivalent changes and modifications are intended to be included within the scope of the present invention without departing from the spirit and scope thereof. The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer. All reagents or equipment were commercially available as conventional products without the manufacturer's attention.
Numerous specific details are set forth in the following description in order to provide a better understanding of the invention. It will be understood by those skilled in the art that the present invention may be practiced without some of these specific details. In other embodiments, methods, means, apparatus and steps well known to those skilled in the art have not been described in detail in order to not obscure the present invention.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. Unless otherwise indicated, all units used in this specification are units of international standard, and the numerical values and numerical ranges appearing in the present invention are understood to include systematic errors unavoidable in industrial production.
Example 1
The embodiment provides a radix stemonae pollen diluent, which comprises the following components: 99.8wt.% water, 0.1wt.% tween 20, 0.1wt.% inositol;
the preparation method comprises the following steps: respectively taking Tween 20 and inositol, adding deionized water for dilution, and dispersing uniformly to obtain radix Stemonae pollen diluent I.
Example 2
The embodiment provides a radix stemonae pollen diluent, which comprises the following components: 99.8wt.% water, 0.2wt.% tween 20.
The preparation method comprises the following steps: and taking Tween 20, adding deionized water for dilution, and uniformly dispersing to prepare the radix stemonae pollen diluent II.
Example 3
The embodiment provides a radix stemonae pollen diluent, which comprises the following components: 99.8wt.% water and 0.1wt.% inositol.
The preparation method comprises the following steps: taking inositol, adding deionized water for dilution, and uniformly dispersing to prepare the stemona pollen diluent III.
Example 4
In this example, the stemona root plant is adopted to test the artificial pollination effect of the stemona root pollen dilution provided in the above examples 1 to 3, and the specific method is as follows:
s1: pollen collection: when the radix stemonae pollen in the month of five months is ripe, placing a paper cup under the flower, gently shaking the radix stemonae flower to enable the pollen to fall into the paper cup, collecting the pollen of 50 plants in total, and mixing;
s2: dispersing the collected pollen with a diluent to form a suspension, comprising:
diluting collected pollen by using the radix stemonae pollen diluent I prepared in the embodiment 1, wherein the mass ratio of the radix stemonae pollen diluent I to the pollen is 200:1 during dilution to prepare suspension I;
diluting the collected pollen by using the radix stemonae pollen diluent II prepared in the embodiment 2, wherein the mass ratio of the radix stemonae pollen diluent II to the pollen is 200:1 during dilution to prepare a suspension II;
diluting collected pollen by using the radix stemonae pollen diluent III prepared in the embodiment 3, wherein the mass ratio of the radix stemonae pollen diluent III to the pollen is 200:1 during dilution to prepare suspension III;
diluting the collected pollen by adopting deionized water, wherein the mass ratio of the deionized water to the pollen is 200:1 during dilution to prepare a mixture IV;
s3: the suspension I, the suspension II, the suspension III and the mixture IV are respectively sucked by a syringe and sprayed on the flower column heads to complete artificial pollination, and the pollination process can be seen in fig. 2. The pollination process of this embodiment is respectively implemented by tester 1, tester 2 and tester 3, each tester respectively operates 200 stemona flowers for pollination, the fruit setting rate is counted after fruit bearing, and the test results are shown in table 1:
TABLE 1 influence of different Stemona pollen dilutions on Stemona plant fruit setting Rate
According to the experimental results, the fruit setting rate of the tween 20 serving as the surfactant is obviously improved compared with that of the tween 20 which is not added into the radix stemonae pollen diluent, and the fruit setting rate of 10d and the fruit setting rate of 70d are not greatly different under the condition that the surfactant and the inositol are simultaneously added, so that the success rate of pollination can be ensured, and the pollination is obviously facilitated.
The above embodiments are only for illustrating the technical solution of the present invention and not for limiting the same, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications and equivalents may be made thereto without departing from the spirit and scope of the technical solution of the present invention, which is intended to be covered by the scope of the claims of the present invention. The technology, shape, and construction parts of the present invention, which are not described in detail, are known in the art.
Claims (10)
1. A radix stemonae pollen diluent, which is characterized in that: comprises a solvent and an additive, wherein the additive at least comprises a surfactant.
2. A radix stemonae pollen dilution as claimed in claim 1, wherein: the surfactant is any one or more of thiobetaine 12, sodium dodecyl benzene sulfonate, polyethylene glycol, alkyl glycoside, tween 80, tween 20 and polyethylene glycol octyl phenyl ether; tween 20 is preferred.
3. A radix stemonae pollen dilution as claimed in claim 1, wherein: the surfactant is present in the diluent at a ratio of from 0.01wt.% to 5wt.%.
4. A radix stemonae pollen dilution as claimed in claim 1, wherein: the solvent is water.
5. A radix stemonae pollen dilution as claimed in claim 1, wherein: the diluent also comprises inositol; preferably, inositol is present in the diluent in a ratio of from 0.01wt.% to 5wt.%, more preferably from 0.01wt.% to 1wt.%.
6. Use of a dilution of stemona pollen as claimed in claim 1 for artificial pollination of plants.
7. An artificial pollination method of radix stemonae, which is characterized by comprising the following steps:
s1: collecting pollen;
s2: dispersing the collected pollen by adopting a diluent to form a suspension, wherein the diluent is the radix stemonae pollen diluent according to any one of claims 1 to 3;
s3: the suspension containing pollen is sucked and dropped or sprayed on the flower column head to complete artificial pollination.
8. The method for artificial pollination of Stemona root of claim 7, wherein: the mass ratio of the diluent to the pollen is 10-1000:1.
9. The method for artificial pollination of Stemona root of claim 7, wherein: the pollen collecting process is used for collecting pollen of at least 2 plants of the same plant and different plants; preferably, pollen from at least 10 different plants of the same plant species is collected; more preferably: at least 30 pollen from different plants of the same plant species were collected.
10. Use of a method of artificial pollination of stemona according to any one of claims 7 to 9 in the propagation of stemona sessilifolia (miq.), stemona sessilifolia (Bl.) miq. Or stemona sessilifolia.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310779099.9A CN116569836A (en) | 2023-06-28 | 2023-06-28 | Stemona pollen diluent and Stemona artificial pollination method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310779099.9A CN116569836A (en) | 2023-06-28 | 2023-06-28 | Stemona pollen diluent and Stemona artificial pollination method |
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| CN116569836A true CN116569836A (en) | 2023-08-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202310779099.9A Pending CN116569836A (en) | 2023-06-28 | 2023-06-28 | Stemona pollen diluent and Stemona artificial pollination method |
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| CN103109737A (en) * | 2013-03-10 | 2013-05-22 | 通化师范学院 | Pollen liquid for high yield of hazelnut fruit and cultivation and pollination of hybrid hazel variety, and preparation method thereof |
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| WO2021202865A1 (en) * | 2020-04-04 | 2021-10-07 | Monsanto Technology Llc | Compositions and methods for liquid-mediated delivery of pollen |
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| US20220338432A1 (en) * | 2019-10-01 | 2022-10-27 | Monsanto Technology Llc | Cross pollination through liquid-mediated delivery of pollen to enclosed stigmas of flowers from recipient plants |
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2023
- 2023-06-28 CN CN202310779099.9A patent/CN116569836A/en active Pending
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| CN103109737A (en) * | 2013-03-10 | 2013-05-22 | 通化师范学院 | Pollen liquid for high yield of hazelnut fruit and cultivation and pollination of hybrid hazel variety, and preparation method thereof |
| CN108834712A (en) * | 2018-06-20 | 2018-11-20 | 怀宁县甘家岭生态林业有限公司 | A kind of implantation methods of high-quality red kiwifruit |
| US20220338432A1 (en) * | 2019-10-01 | 2022-10-27 | Monsanto Technology Llc | Cross pollination through liquid-mediated delivery of pollen to enclosed stigmas of flowers from recipient plants |
| WO2021202865A1 (en) * | 2020-04-04 | 2021-10-07 | Monsanto Technology Llc | Compositions and methods for liquid-mediated delivery of pollen |
| US20210307273A1 (en) * | 2020-04-04 | 2021-10-07 | Monsanto Technology Llc | Compositions and methods for liquid-mediated delivery of pollen |
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