CN103380785A - Wheat chemical emasculation agent and application thereof in wheat hybrid seed production - Google Patents
Wheat chemical emasculation agent and application thereof in wheat hybrid seed production Download PDFInfo
- Publication number
- CN103380785A CN103380785A CN2013103326253A CN201310332625A CN103380785A CN 103380785 A CN103380785 A CN 103380785A CN 2013103326253 A CN2013103326253 A CN 2013103326253A CN 201310332625 A CN201310332625 A CN 201310332625A CN 103380785 A CN103380785 A CN 103380785A
- Authority
- CN
- China
- Prior art keywords
- wheat
- leaf
- cavity
- chemical emasculation
- male
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a wheat chemical emasculation agent and an application thereof in wheat hybrid seed production and belongs to the technical field of plant breeding. According to the wheat chemical emasculation agent, organosilicon surfactant polyether modified heptamethyl trisiloxane (Silwet L-77) is prepared with distilled water into a solution with the concentration of 0.025%-0.075% (v/v). The usage method of the wheat chemical emasculation agent includes selecting wheat ears with a distance of 0.5-5 centimeters between the flag leaf and the second top leaf, injecting the wheat chemical emasculation agent into a cavity above every young ear through an injector and filling the cavity as much as possible. The wheat chemical emasculation agent has an emasculation abortion rate of 73% without affecting the fertility of the pistil and further the growth of the whole plant; after simple headed ear preparation, the pistil can be pollinated manually with pollen of other wheat species and bagged, accordingly hybrid seeds can be harvested after field management with conventional methods; normal hybrid seeding and a hybrid rate of 100% can be achieved. The wheat chemical emasculation agent and the application method thereof are simple, low in production costs, environmentally friendly and easy to popularize.
Description
(1) technical field
The present invention relates to a kind of wheat male sterilant and the application in the wheat hybridizing breeding thereof, belong to field of plant variety breeding technology.
(2) background technology
Utilize the hybrid vigour of crop can carry out the improvement of crop, as improving crop yield, strengthening cropper resistance etc.The method is that the first generation cross-fertilize seed that relies on pure lines hybridization is realized.The crops such as corn, rape, paddy rice, Chinese sorghum have extensively utilized hybrid vigour to carry out the production of hybrid seeds at present.
Wheat is distribute in the world the widest and have large heterotic second largest cereal crops, and various countries are all making great efforts to seek to develop its heterotic approach and method always.Wheat heterosis utilizes the breakthrough of theoretical and application technology, not only will greatly enrich crop heterosis and utilize theory and technology, and will make significant contribution to the grain security production in China and even the world.Wheat is self pollination crop, and therefore, the key problem of wheat heterosis utilization is to set up the technical system of High-efficient Production wheat hybrid.The utilization of Chemical Castration Technology (method is killed in change) is to solve one of effective ways of maternal breeding emasculation problem.The chemical emasculation method is to utilize medicine (chemical hybridizing agent) that the male flower of wheat is killed, and makes it can not produce pollen, but gynoecium preserves from, thereby crossbreed is produced in inducing wheat male sterile.The chemical emasculation method is utilized wheat heterosis, and maximum advantage is that combo is free, and strong excellent combination elimination factor is high; There is no the cytoplasm negative effect; The seed production routine is simple; Even hybrid purity is not bery high, also not too large etc. to yield effect.
More research is being done by some developed countries such as English, U.S., Fa Zhu state aspect the male sterilant screening, particularly T-shaped male sterile line of the middle and later periods seventies is in the U.S. and other various countries in succession after failure, the screening study of wheat male sterilant has entered climax, the middle and later periods eighties, various countries have filtered out some male sterilants in succession, and core technology and patent are mainly grasped by the trans-corporation of the U.S. and France.
China has just begun the research of chemical hybridizing agent and application thereof from the eighties in 20th century, independent research goes out male sterilant BAU-9403 (China Agricultural University) and SQ-1 (Xibei Univ. of Agricultural ﹠ Forest Science ﹠ Technology).Production of hybrid seeds effect depends on spray technique, and effective field hybrid seed yield is high, hybrid purity is high.But some fields or owing to producing poisoning, hybrid seed yield is low, perhaps because the spray medicine is not in place, hybrid purity is low.
It is SC2053 and genesis that present most popularization killed agent.SC2053 be academy of agricultural sciences, Tianjin crop with the product of U.S. Sogetal company cooperative development, spraying period is that the wheat Pistil And Stamen is formed to the connective phase (this period morphological index be l centimetre of left and right of stem children's spike length).Gametocidal effect is good, and after gametocide, maternal outcrossing characteristic is improved.The shortcoming of this medicament maximum is that spray medicine operation requirements is very strict, otherwise causes poisoning.
Genesis is the new product of About Monsanto Chemicals.The advantage of this male gametocide is that spraying concentration elasticity is larger, is difficult for causing poisoning and gametocide thorough.Its shortcoming is spraying period more late (booting stage), is not easy to do mechanization operation, except SC2053 and genesis, and China has also developed voluntarily some changes and killed agent, as EK, the ES of Hebei Normal University's development; The BAU-2 of China Agricultural University's development etc.
The polyether-modified heptamethyltrisiloxane of organic silicon surfactant (Silwet L-77) is produced by U.S. GE company, Plant Transformation auxiliary reagent and the insecticides adjuvants such as Chang Zuowei arabidopsis, rape.Can reduce the surface tension of water as insecticides adjuvant, reduce the contact angle of liquid and biological target, have resistance of rainwater washing against and infiltration capacity, improve the effective rate of utilization of agricultural chemicals and meet environmental requirement.After adding the Silwet surfactant in liquid, liquid can be absorbed by plant stomata, thereby increases drug effect.
Chinese patent file publication number CN1596617A discloses a kind of method and application of setting up the annual bluegrass genetic conversion system.The sprout tuber that grows thickly of getting the suitable growth phase is that acceptor adopts particle bombardment or agrobacterium-mediated transformation to carry out genetic transformation, obtains transfer-gen plant.In agriculture bacillus mediated genetic transformation, adopt surfactant Silwet L-77 to process and reduced pressure treatment has effectively improved transformation frequency.
Chinese patent file publication number CN101099497A discloses a kind of water soluble insecticide preparation, and this invention relates to the water soluble insecticide preparation that contains the agricultural spray adjuvants of Silwet and their preparation method.Fast, the easy degraded of this water soluble preparation dissolving, noresidue, free from environmental pollution are environmentally friendly pesticidal preparations.
Up to now, do not see the report that utilizes the polyether-modified heptamethyltrisiloxane of organic silicon surfactant (Silwet L-77) preparation wheat male sterilant and use in the wheat hybridizing breeding.
Some application have been obtained although utilize chemical emasculation method large tracts of land to produce first generation cross-fertilize seed on the crops such as wheat, the problem such as this application process but exists many defectives, and is long such as the activity duration, that cost is high, poisoning is arranged, gametocidal effect is undesirable.
(3) summary of the invention
The present invention is directed to the deficiencies in the prior art, a kind of wheat male sterilant and the application in the wheat hybridizing breeding thereof are provided, it has made up the deficiency of existing wheat chemical emasculation and crossbreeding operation.
The object of the present invention is achieved like this.Through a large amount of laboratory test and for many years composition and the proportioning thereof of the definite wheat male sterilant of field trial be expressed as follows:
The polyether-modified heptamethyltrisiloxane of organic silicon surfactant (Silwet L-77) is mixed with distilled water the solution that concentration is 0.025%-0.075% (V/V), without adjust pH.
The preparation method of wheat male sterilant of the present invention comprises the steps:
The polyether-modified heptamethyltrisiloxane of organic silicon surfactant (Silwet L-77) is mixed with distilled water the solution that concentration is 0.025%-0.075% (V/V), without adjust pH.
Utilize the method step of above-mentioned male sterilant as follows:
(1) grow all that leaf slightly (is chosen flag) and boot leaf and the auricle of two leaves of falling operate apart from for 0.5-5 centimetre the time until wheat flag leaf, the cavity of wheat children tassel and young fringe top is arranged in the cylinder that this moment, the boot leaf leaf sheath was rolled into.Preparation is processed the wheat head with male gametocide.
(2) draw above-mentioned male sterilant with a clean syringe, cavity is inserted in the top of the cylinder that syringe needle is rolled into from the boot leaf leaf sheath, and male gametocide is injected in cavity, cavity is full of as far as possible, liquid has just been overflowed from top be advisable, and does not damage the wheat head.
(3) wheat head that will inject is carried out mark, allows its self-sow.
(4) after the heading, can find that the wheat head color through injecting is yellowish green, and undressed wheat head color is dark.The obvious wheat head of the phenomenon that gets colors after simple whole fringe, is manually awarded with the normal pollen of other wheat, and bagging carries out field management according to a conventional method, can gather in the crops hybrid seed.
Described is gramineous plants, preferred common wheat.
Beneficial effect of the present invention is as follows
1, utilize the concentration of SilwetL-77 to be 0.025%-0.075%(V/V) male sterilant, for field of wheat breeding provides a kind of simple, effective new male gametocide.The color of the sterile fringe of processing is obvious with normal wheat head color distortion, easily distinguishes.
2, thereby the present invention directly injects the wheatear in booting stage and obtains a large amount of sterile wheat heads, has greatly saved the time of artificial emasculation.
The abortion rate of the wheatear of 3, processing through male gametocide of the present invention reaches 73%, and other proterties are constant, for the crossbreeding of wheat provides good condition.
4, male gametocide active ingredient of the present invention is surfactant, is not mutagen, and is harmless to the person poultry safety.Directly the method for injection can contaminated soil yet.
Description of drawings:
Fig. 1. the wheat head (right side) that contrast (left side) and the male sterilant through containing Silwet L-77 are processed.It is shallow that the rear wheat head color of processing contrasts tassel, and clever shell all opens.
Fig. 2. the Xiao Hua (right side) on the wheat head that normal Xiao Hua (left side) and the male sterilant through containing Silwet L-77 are processed is relatively.Contrast Xiao Hua also has 3 yellowish green flower pesticide except normal fine hair shape gynoecium is arranged, and the Xiao Hua that processes through male gametocide can see normal fine hair shape gynoecium, but 3 all atrophy degenerations of flower pesticide.
Fig. 3. the TTC dyeing of the flower pesticide of the abortion that the male sterilant through containing Silwet L-77 is processed is observed.Dyeing shows Anther degeneration, the inner pollen that there is no survival.
Fig. 4. the seed of tying after the wheat head of the safe agriculture 18 of wheat that the male sterilant that contains Silwet L-77 is processed is manually awarded pollen with blue-grain wheat.Be followed successively by from left to right safe agriculture 18, crossbreed, blue-grain wheat.
Embodiment
Below in conjunction with embodiment, the present invention is described in detail.
Wheat lines CB037 in embodiment and Tai Nong 18 are the wheat breed (being) of country of Shandong Agricultural University wheat improvement branch center, Tai'an, center preservation.Silwet L-77 is available from GE company.
Embodiment 1
A kind of wheat male sterilant, composition are that concentration is 0.025%-0.075% (V/V), without adjust pH with the Silwet L-77 solution of distilled water preparation.
Embodiment 2
This experiment be experimental field base, Shandong Agricultural University's experimental teaching of agronomy station.
Utilize the method for aforesaid a kind of wheat male sterilant gametocide, step is as follows:
(1) wheat line CB037 is seeded into the experimental field, carries out according to a conventional method field management.
(2) wheat enters booting stage, chooses that boot leaf grows all that leaf slightly (is chosen flag) and boot leaf and the plant of auricle apart from for 0.5-5 centimetre time of two leaves of falling, and begins that cavity is arranged in the cylinder that this moment, the boot leaf leaf sheath was rolled into.Can easily touch with hand.
(3) draw above-mentioned male sterilant with the syringe of a cleaning, cavity is inserted in the top of the cylinder that syringe needle is rolled into from the boot leaf leaf sheath, and male gametocide is injected in cavity, cavity is full of as far as possible, liquid has just been overflowed from top be advisable.
(4) wheat head that will inject is carried out mark, bagging.After results, statistics abortion rate.
Abortion rate (%)=stamen abortion tassel number/always process tassel number * 100%
More of light color than normal tassel when the tassel after processing has just been released, inclined to one side yellow green, clever shell opens gradually, and the Stigma shape normal energy is accepted foreign pollen.
Being that the wheat head abortion rate that 0.025% male sterilant is processed is 56% through measuring and calculating through silwet L-77 concentration, is that the wheat head abortion rate that 0.05% male sterilant is processed is 73.9% through silwet L-77 concentration.
Embodiment 3
Utilize male sterilant to carry out wheat crossbreeding.The safe agriculture 18 of wheat breed is seeded into the experimental field, carries out according to a conventional method field management.Wheat enters booting stage, can touch the cavity at tassel position with hand, begins to process.Needle tubing with a cleaning is drawn above-mentioned male sterilant, choose that boot leaf grows all that leaf slightly (is chosen flag) and boot leaf and the wheat head of auricle apart from for 0.5-5 centimetre time of two leaves of falling, insert in cavity at the cavity top that syringe needle is rolled into from the boot leaf leaf sheath, and male gametocide is injected into the cavity on tassel top, cavity is full of as far as possible, liquid has just been overflowed from top be advisable.After the tassel of processing put forth ears, obvious 10 wheat heads of the phenomenon that gets colors after simple whole fringe, were manually awarded the pollen with blue-grain wheat, and bagging carries out field management according to a conventional method.Add up the cross-fertile rate after collecting seed.
Cross-fertile rate (%)=mature grain number/pollination Xiao Hua number * 100%.
The cross-fertile rate of statistical result showed after male sterilant is processed is 100%.
Claims (2)
1. a wheat male sterilant, is characterized in that it is formulated with distilled water with the polyether-modified heptamethyltrisiloxane Silwet of organic silicon surfactant L-77, and concentration is 0.025%-0.075% (v/v).
2. method of utilizing the wheat male sterilant claimed in claim 1 production of hybrid seeds, step is as follows:
Grow all that leaf slightly (is chosen flag) and boot leaf and the auricle of two leaves of falling operate apart from for 0.5-5 centimetre the time until wheat flag leaf, the cavity of wheat children tassel and young fringe top is arranged in the cylinder that this moment, the boot leaf leaf sheath was rolled into; Draw above-mentioned male sterilant with a clean syringe, cavity is inserted in the top of the cylinder that syringe needle is rolled into from the boot leaf leaf sheath, and male gametocide is injected in cavity, cavity is full of as far as possible, liquid has just been overflowed from top be advisable, and does not damage the wheat head; The wheat head of injecting is carried out mark, allow its self-sow; After heading, can find that the wheat head color through injecting is yellowish green, and undressed wheat head color is dark; The obvious wheat head of the phenomenon that gets colors after simple whole fringe, is manually awarded with the normal pollen of other wheat, and bagging carries out field management according to a conventional method, can gather in the crops hybrid seed.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013103326253A CN103380785A (en) | 2013-08-01 | 2013-08-01 | Wheat chemical emasculation agent and application thereof in wheat hybrid seed production |
| CN201410363718.7A CN104094964B (en) | 2013-08-01 | 2014-07-28 | Silwet L-77 is as the application in wheat male sterilant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013103326253A CN103380785A (en) | 2013-08-01 | 2013-08-01 | Wheat chemical emasculation agent and application thereof in wheat hybrid seed production |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103380785A true CN103380785A (en) | 2013-11-06 |
Family
ID=49488876
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013103326253A Pending CN103380785A (en) | 2013-08-01 | 2013-08-01 | Wheat chemical emasculation agent and application thereof in wheat hybrid seed production |
| CN201410363718.7A Expired - Fee Related CN104094964B (en) | 2013-08-01 | 2014-07-28 | Silwet L-77 is as the application in wheat male sterilant |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410363718.7A Expired - Fee Related CN104094964B (en) | 2013-08-01 | 2014-07-28 | Silwet L-77 is as the application in wheat male sterilant |
Country Status (1)
| Country | Link |
|---|---|
| CN (2) | CN103380785A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106577263A (en) * | 2016-12-19 | 2017-04-26 | 陕西省杂交油菜研究中心 | Precision application method for chemical hybridization agent for rapes |
| CN107926974A (en) * | 2017-11-21 | 2018-04-20 | 浙江世佳科技有限公司 | Polyether-modified heptamethyltrisiloxane organosilicon is used to prepare the purposes of the pesticide of prevention crop pest |
| CN116508645A (en) * | 2023-04-17 | 2023-08-01 | 安新县益农农业科技有限公司 | Crossbreeding method of new wheat strain |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1597933A (en) * | 2004-08-13 | 2005-03-23 | 山东大学 | Process for construvting transgene teceptor system of rye grass and its application |
| CN1322136C (en) * | 2004-08-06 | 2007-06-20 | 山东大学 | Method of establishing early-maturing ripe hereditary transform system and application |
| CN102212552A (en) * | 2011-05-06 | 2011-10-12 | 西南大学 | Method for transforming genes by using efficient living body of chemical male sterilant |
-
2013
- 2013-08-01 CN CN2013103326253A patent/CN103380785A/en active Pending
-
2014
- 2014-07-28 CN CN201410363718.7A patent/CN104094964B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1322136C (en) * | 2004-08-06 | 2007-06-20 | 山东大学 | Method of establishing early-maturing ripe hereditary transform system and application |
| CN1597933A (en) * | 2004-08-13 | 2005-03-23 | 山东大学 | Process for construvting transgene teceptor system of rye grass and its application |
| CN102212552A (en) * | 2011-05-06 | 2011-10-12 | 西南大学 | Method for transforming genes by using efficient living body of chemical male sterilant |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106577263A (en) * | 2016-12-19 | 2017-04-26 | 陕西省杂交油菜研究中心 | Precision application method for chemical hybridization agent for rapes |
| CN106577263B (en) * | 2016-12-19 | 2019-04-05 | 陕西省杂交油菜研究中心 | A kind of rape chemical hybridizing agent accuracy pesticide applying method |
| CN107926974A (en) * | 2017-11-21 | 2018-04-20 | 浙江世佳科技有限公司 | Polyether-modified heptamethyltrisiloxane organosilicon is used to prepare the purposes of the pesticide of prevention crop pest |
| CN116508645A (en) * | 2023-04-17 | 2023-08-01 | 安新县益农农业科技有限公司 | Crossbreeding method of new wheat strain |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104094964A (en) | 2014-10-15 |
| CN104094964B (en) | 2016-04-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105746334B (en) | It is a kind of to promote the solid gynoecium reagent treatment of Wide_cross in Plant and its application process | |
| CN101828554B (en) | Pruning synergist for cotton | |
| CN104770174A (en) | High-quality and high-yield planting method of oil peonies | |
| CN107135950A (en) | A kind of breeding method of quick acquisition black fruit fructus lycii regrowth | |
| CN103125386B (en) | Industrial horseradish planting method | |
| CN103430833B (en) | A kind of paddy rice sexual hybridization gametocide new method | |
| CN104094964B (en) | Silwet L-77 is as the application in wheat male sterilant | |
| CN106172481A (en) | A kind of plant seed developmental inhibitors for controlling weeds and application thereof | |
| CN101238792A (en) | Single pistillate castor vegetative propagation method | |
| CN110140564A (en) | Utilize the method for photosynthetic film prevention and control tobacco virus high in fat | |
| CN1306864C (en) | Method for modifying sweet potato hybridisation setting percentage and its special treating agent | |
| CN103222423B (en) | Rice hybrid breeding material as well as cultivation method and application thereof | |
| CN1524417A (en) | Chemical crossbred agent composition, its using method and application | |
| CN103250632B (en) | Propagation method for tobacco seeds by artificial supplementary pollination | |
| CN110074111A (en) | A kind of inorganic agent and method improving millet Cross fertile rate | |
| CN113558061B (en) | Active component of Holotrichia parallela trapping agent, trapping agent and trapping method | |
| CN102057863A (en) | Method for producing cotton seeds by inducing long cotton stigmas | |
| CN106342680B (en) | A method of being suitable for Multi-genotype Wheat mitochondria culture | |
| CN108157051A (en) | A kind of implantation methods of mulberry tree | |
| CN107417440A (en) | A kind of Pesticidal combination and its application containing biogas slurry | |
| CN106416654A (en) | Corn southern leaf blight field natural inducement technology and application of technology | |
| Nakasone et al. | Artificial induction of polyploidy in orchids by the use of colchicine | |
| CN111264379A (en) | Banana cultivation method convenient for artificial pollination | |
| CN105613259A (en) | Selecting and breeding method for bacterial wilt-resistant sweet potato variety | |
| CN102405829A (en) | Hybrid cotton seed production method through spraying flowers with clean water to realize emasculation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20131106 |