CN116172194B - Health food containing broad bean pod extract and application thereof - Google Patents
Health food containing broad bean pod extract and application thereof Download PDFInfo
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- CN116172194B CN116172194B CN202111428447.5A CN202111428447A CN116172194B CN 116172194 B CN116172194 B CN 116172194B CN 202111428447 A CN202111428447 A CN 202111428447A CN 116172194 B CN116172194 B CN 116172194B
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- 235000010749 Vicia faba Nutrition 0.000 title claims abstract description 16
- 240000006677 Vicia faba Species 0.000 title claims abstract description 16
- 235000002098 Vicia faba var. major Nutrition 0.000 title claims abstract description 16
- 229940095612 bean pod extract Drugs 0.000 title claims description 7
- 235000013402 health food Nutrition 0.000 title abstract description 4
- 239000000284 extract Substances 0.000 claims abstract description 76
- 235000014360 Punica granatum Nutrition 0.000 claims abstract description 12
- 241000255789 Bombyx mori Species 0.000 claims abstract description 6
- 244000294611 Punica granatum Species 0.000 claims abstract 4
- 210000000582 semen Anatomy 0.000 claims description 21
- 239000000203 mixture Substances 0.000 claims description 19
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 13
- 238000002360 preparation method Methods 0.000 claims description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- 235000013305 food Nutrition 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 238000009835 boiling Methods 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 238000005520 cutting process Methods 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 239000000706 filtrate Substances 0.000 claims description 3
- 238000002386 leaching Methods 0.000 claims description 3
- 238000010298 pulverizing process Methods 0.000 claims description 3
- 238000007873 sieving Methods 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims description 3
- 238000001694 spray drying Methods 0.000 claims description 3
- 239000004480 active ingredient Substances 0.000 claims 1
- 238000004108 freeze drying Methods 0.000 claims 1
- 208000027089 Parkinsonian disease Diseases 0.000 abstract description 7
- 206010034010 Parkinsonism Diseases 0.000 abstract description 7
- 229930003935 flavonoid Natural products 0.000 abstract description 5
- 235000017173 flavonoids Nutrition 0.000 abstract description 5
- 150000002215 flavonoids Chemical class 0.000 abstract description 5
- VYFYYTLLBUKUHU-UHFFFAOYSA-N dopamine Chemical compound NCCC1=CC=C(O)C(O)=C1 VYFYYTLLBUKUHU-UHFFFAOYSA-N 0.000 abstract description 3
- 238000000605 extraction Methods 0.000 abstract description 3
- 239000005445 natural material Substances 0.000 abstract description 3
- 230000002195 synergetic effect Effects 0.000 abstract description 3
- 150000008442 polyphenolic compounds Chemical class 0.000 abstract description 2
- 235000013824 polyphenols Nutrition 0.000 abstract description 2
- 150000004670 unsaturated fatty acids Chemical class 0.000 abstract description 2
- 235000021122 unsaturated fatty acids Nutrition 0.000 abstract description 2
- 239000002699 waste material Substances 0.000 abstract description 2
- 241000699670 Mus sp. Species 0.000 description 19
- 241000219991 Lythraceae Species 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 8
- 208000013738 Sleep Initiation and Maintenance disease Diseases 0.000 description 7
- 206010022437 insomnia Diseases 0.000 description 7
- 238000010172 mouse model Methods 0.000 description 5
- DIVDFFZHCJEHGG-UHFFFAOYSA-N oxidopamine Chemical compound NCCC1=CC(O)=C(O)C=C1O DIVDFFZHCJEHGG-UHFFFAOYSA-N 0.000 description 5
- PLRACCBDVIHHLZ-UHFFFAOYSA-N 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine Chemical compound C1N(C)CCC(C=2C=CC=CC=2)=C1 PLRACCBDVIHHLZ-UHFFFAOYSA-N 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 230000005764 inhibitory process Effects 0.000 description 4
- 239000002504 physiological saline solution Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- NIGWMJHCCYYCSF-UHFFFAOYSA-N Fenclonine Chemical compound OC(=O)C(N)CC1=CC=C(Cl)C=C1 NIGWMJHCCYYCSF-UHFFFAOYSA-N 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 238000004113 cell culture Methods 0.000 description 3
- 230000009194 climbing Effects 0.000 description 3
- 239000007928 intraperitoneal injection Substances 0.000 description 3
- HLHSUNWAPXINQU-GQCTYLIASA-N (E)-3-(3,4-dihydroxyphenyl)-N-prop-2-ynylprop-2-enamide Chemical compound OC=1C=C(C=CC=1O)/C=C/C(=O)NCC#C HLHSUNWAPXINQU-GQCTYLIASA-N 0.000 description 2
- 208000035823 Non-specific autoimmune cerebellar ataxia without characteristic antibodies Diseases 0.000 description 2
- 230000003542 behavioural effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000002496 gastric effect Effects 0.000 description 2
- 230000001766 physiological effect Effects 0.000 description 2
- 229920000771 poly (alkylcyanoacrylate) Polymers 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- 206010003210 Arteriosclerosis Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- 238000000134 MTT assay Methods 0.000 description 1
- 231100000002 MTT assay Toxicity 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 208000001132 Osteoporosis Diseases 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 208000011775 arteriosclerosis disease Diseases 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 229920002770 condensed tannin Polymers 0.000 description 1
- 239000007799 cork Substances 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000008176 lyophilized powder Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000011177 media preparation Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
- A61P25/16—Anti-Parkinson drugs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/20—Hypnotics; Sedatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/31—Extraction of the material involving untreated material, e.g. fruit juice or sap obtained from fresh plants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- Neurosurgery (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Neurology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Epidemiology (AREA)
- General Chemical & Material Sciences (AREA)
- Medical Informatics (AREA)
- Biotechnology (AREA)
- Alternative & Traditional Medicine (AREA)
- Organic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Psychology (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Anesthesiology (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a health food containing extract of pod of silkworm, which is matched with 5% -20% of extract of pomegranate seeds. The invention uses the waste broad bean pods, can obtain the extract with use value through extraction, is matched with the pomegranate seed extract, plays a synergistic effect in organic combination of the contained natural substances such as procyanidine, L-dopamine, flavonoid, polyphenol, unsaturated fatty acid and the like, can assist in improving sleep while treating parkinsonism, further improves the original efficacy of broad beans, and provides a thinking for developing a new health food.
Description
Technical Field
The invention relates to application of broad bean pod extract, in particular to health-care food containing the broad bean pod extract and a preparation method thereof.
Background
In recent years, the research on the physiological activities of levodopamine, procyanidine and flavonoid substances extracted from broad beans is more and more increasing, the procyanidine has the physiological activities of antioxidation, bacteriostasis, anticancer, mutation resistance and the like, the flavonoid substances also have the effects of reducing blood fat, resisting arteriosclerosis, resisting tumors, resisting osteoporosis and the like, and people always have the habit of eating the broad beans to relieve the parkinsonism.
The pod of the broad beans can not be eaten generally, but the research discovers that the content of effective nutrient substances like proanthocyanidins, L-dopamine, flavonoid substances and the like in the pod of the broad beans is not low, so the invention fully utilizes the pod of the broad beans, and the available extract can be obtained through the extraction process of the invention.
Disclosure of Invention
Through a large number of experiments, the specific efficacy can be enhanced by adding the pomegranate seed extract with a certain proportion into the pod extract of the silkworm, and unexpected effects can be exerted.
A health product contains semen Viciae Fabae pod extract and semen Granati extract, wherein the proportion of semen Granati extract is 5-20% (W/W).
The preparation method of the pod extract comprises the following steps:
cleaning the pod of the silkworm with clear water, removing rotten and deteriorated parts, cutting into small segments, squeezing with a juicer, boiling the juice for 3-5 min, sieving with a 400-mesh sieve, removing filter residues, and collecting filtrate to obtain the original juice of the extract. Concentrating the raw juice in vacuum drier with vacuum degree of 1-5kPa and temperature of no more than 45deg.C to below 50% of the weight of the juice, and lyophilizing to obtain extract of herba Viciae Fabae pod.
The preparation method of the pomegranate seed extract comprises the following steps:
collecting semen Granati, drying, pulverizing, adding 10 times of 75% ethanol, soaking for 30min, leaching at 60-70deg.C for 2 times, each time for 1 hr, standing, centrifuging, concentrating to obtain extract of 1.3-1.4, and spray drying to obtain semen Granati extract.
Mixing semen Viciae Fabae pod extract with semen Granati extract, wherein the ratio of semen Granati extract is 5-20% (W/W).
The beneficial technical effects of the invention are mainly embodied in the following aspects:
(1) A preparation method of extract containing semen Viciae Fabae pod extract and semen Granati extract is provided.
(2) According to the combination ratio of the two extracts in the invention, experiments show that the extract mixture has a recovery effect on a parkinsonism cell model (6-OHDA modeling, PC12 cells and SH-SY5Y cells) and can reduce the inhibition effect of the 6-OHDA on the cells.
According to the combination ratio of the two extracts, experiments show that the extract mixture prepared by the invention can improve the behavior state of mice to different degrees after the extract mixture prepared by the invention and physiological saline are respectively given to an MPTP (MPTP is injected into 1-methyl-4-phenyl-1, 2,3, 6-tetrahydropyridine, the same applies below) induced parkinsonism mouse model, and the extract mixture has obvious therapeutic effect on parkinsonism model mice.
(3) According to the combination ratio of the two extracts, experiments show that the mixture of the extracts has obvious reduction of insomnia rate of mice with model insomnia caused by Phenylalanine (PACA).
(4) Compared with the prior art, the waste broad bean pods are used, the extracts with use value can be obtained through extraction, and the extracts are matched with the pomegranate seed extracts, so that the extracts play a synergistic effect in organic combination of natural substances such as procyanidine, L-dopamine, flavonoid, polyphenols, unsaturated fatty acid and the like, and the novel health care food can assist in improving sleep while treating parkinsonism, further improves the original efficacy of broad beans, and provides a thought for developing a novel health care food.
Detailed Description
Example 1
Comprises concentrated juice of herba Violae extract and lyophilized powder.
Cleaning the pod of the silkworm with clear water, removing rotten and deteriorated parts, cutting into small segments, squeezing with a juicer, boiling the juice for 3-5 min, sieving with a 400-mesh sieve, removing filter residues, and collecting filtrate to obtain the original juice of the extract. Concentrating the raw juice in a vacuum drier with vacuum degree of 1-5kPa and temperature less than or equal to 45deg.C until the weight of the juice is less than 50%, and lyophilizing to obtain semen Viciae Fabae pod extract.
The preparation method of the pomegranate seed extract comprises the following steps:
collecting semen Granati, drying, pulverizing, adding 10 times of 75% ethanol, soaking for 30min, leaching at 60-70deg.C for 2 times, each time for 1 hr, standing, centrifuging, concentrating to obtain extract of 1.3-1.4, and spray drying to obtain semen Granati extract.
Mixing semen Viciae Fabae pod extract with semen Granati extract at a ratio of 10% (W/W) to obtain extract mixture.
Example 2
Cell proliferation was detected by MTT assay:
1 cell digestion, counting, preparing cell suspension with concentration of 5X 10 4/mL, adding 100 mu L of cell suspension into each well of a 96-well cell culture plate;
2, placing the 96-well cell culture plate in a culture box with 5% CO 2 at 37 ℃ for culturing for 24 hours;
3 diluting to the required concentration with complete culture medium, adding 100 μl of corresponding medicated culture medium (medicated culture medium preparation: taking mixture of semen Viciae Fabae pod extract and semen Granati extract (9:1) 1 g-10 ml volumetric flask, dissolving in water and fixing volume), and setting negative control group;
4, placing the 96-well cell culture plate in a culture box with 5% CO 2 for culturing for 24 hours, and then adding 6-OHDA for molding for 24 hours;
5 MTT staining was performed on 96-well plates, λ=490 nm, and OD values were determined;
a20. Mu.L MTT (5 mg/mL) was added to each well, and the culture was continued in an incubator for 4 hours;
B, discarding the culture medium, adding 150 mu L of DMSO into each hole for dissolution, and gently mixing by a shaking table for 10 min;
C lambda=490 nm, the enzyme label instrument reads out the OD value of each hole, and the inhibition rate is calculated;
6 count of inhibition ratios of groups
From the data, it can be seen that: the extract has recovery effect on 6-OHDA model cell (Parkinson cell model), and can reduce inhibition effect of 6-OHDA on cell production.
Example 3
Mouse behavioural experiments
Experimental grouping: adult mice were taken in 24 groups of 4, 6 in each group, blank (saline 1-18 d), model (saline 1-8d, MPTP9-18 d), and dosing group (extract 1-18d, MPTP9-18 d).
Dosage and mode of administration: normal saline (lavage): 30mg/kg/d
Extract mixture (lavage): high dose 500mg/kg extract mixture, low dose: 300mg/kg of extract mixture prepared as in example 1
MPTP (intraperitoneal injection): 30mg/kg/d
Frequency of administration: once a day
The behavioral method is used for detecting:
Pole climbing experiment: a smooth wood rod with a diameter of 1cm and a height of 50cm and a wood ball with a diameter of 3cm at the top is taken. The mice to be tested were placed on cork pellets during the test, and the time required for the mice to climb to the bottom of the pole was recorded and statistically analyzed. All mice were assayed for pole climbing time on day 8 of dosing and again after 1h of dosing on day 18.
Rotating shaft experiment: the mice were placed on the rotating stick and the time from the beginning of rotation of the test mice to the falling off the rotating stick was measured. Spindle time was measured for all mice on day 8 of dosing and for 1 hour after dosing on day 18.
Grip strength experiment: the tested mice are placed on a tensile tester, the tail parts of the mice are grasped, the mice are pulled backwards in a straight line, and the grabbing force data are recorded. All mice were measured for grip size on day 8 of dosing and their tension again after dosing on day 18.
Efficacy results:
from the data, it can be seen that: through the Parkinson model of the mice, the administration group can obviously increase the pole climbing time and the rotating shaft time of the mice in the model group and increase the pulling force of the mice. The extract mixture is shown to have obvious therapeutic effects on parkinsonism model mice.
Example 4
Test of Insomnia Rate in mice
Adult healthy mice 73, 6 groups of 11-13 animals each, control group (physiological saline 1-9 d), model group (PCPA-2 d, physiological saline 3-9 d), experimental group (PCPA-2 d, extract mixture 3-9 d), pod extract group (PCPA-2 d, pod extract 3-9 d), pomegranate seed extract group (PCPA-2 d, pomegranate seed extract 3-9 d)
Dosage and mode of administration:
normal saline (intraperitoneal injection/lavage): 30mg/kg/d
Extract mixture (lavage): high dose 500mg/kg extract mixture, low dose: 300mg/kg of extract mixture prepared as in example 1
Pod extract (lavage): 500mg/kg of extract prepared in example 1
Pomegranate seed extract (lavage): 500mg/kg of extract prepared in example 1
PCPA (intraperitoneal injection): 300mg/kg/d
Frequency of administration: once a day
On the 1 st to 2 nd days of the test, the mice were observed for insomnia after 45min of administration by injecting PCPA intraperitoneally, 1 time a day, 2 nd day, of each group except the control group animals. After successful modeling, the control group and the model group are subjected to gastric lavage and administration of the same amount of physiological saline, the experimental group is subjected to gastric lavage and administration, the animal falls asleep after 45min of administration is observed, and the insomnia rate of the 3-9 balance is counted.
From the data, it can be seen that: the insomnia model mice caused by the p-chlorophenylalanine (PACA) are respectively provided with the extract mixture, the single broad bean pod extract and the single pomegranate seed extract, and the extract mixture can obviously reduce the insomnia rate of the model mice compared with the single extract, and the effect is better as the dosage is increased. It is shown that the extract mixture may exert a synergistic effect due to the complementation of the two natural substances, which is more beneficial to improving sleep.
Claims (1)
1. The application of a composition with active ingredients of broad bean pod extract and pomegranate seed extract in preparing health-care food for improving sleep is characterized in that the preparation method of the broad bean pod extract is as follows: cleaning the pod of the silkworm with clear water, removing rotten and deteriorated parts, cutting into small sections, squeezing with a juicer, boiling the juice for 3-5 min, sieving with a 400-mesh sieve, removing filter residues, collecting filtrate to obtain extract raw juice, concentrating the raw juice to below 50% of the weight of the juice in a vacuum drier with vacuum degree of 1-5kPa and temperature of less than or equal to 45 ℃, and freeze-drying to obtain the extract of pod of the silkworm; the preparation method of the pomegranate seed extract comprises the following steps: collecting semen Granati, drying, pulverizing, adding 10 times of 75% ethanol, soaking for 30min, leaching at 60-70deg.C for 2 times, each time for 1 hr, standing, centrifuging, concentrating to obtain extract of 1.3-1.4, and spray drying to obtain semen Granati extract; mixing semen Viciae Fabae pod extract with semen Granati extract at a ratio of 5-20% (W/W).
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| CN105533686A (en) * | 2015-12-10 | 2016-05-04 | 齐鲁工业大学 | Pomegranate seed powder medicinal granules |
| CN109091609A (en) * | 2018-11-01 | 2018-12-28 | 苏州卫生职业技术学院 | A kind of Chinese medicine composition and preparation method thereof with anti-oxidant blood fat reducing function |
| CN109303333A (en) * | 2018-09-12 | 2019-02-05 | 珠海霍普金斯医药研究院股份有限公司 | A kind of Halth-care composition improving sleep |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1785218A (en) * | 2005-11-22 | 2006-06-14 | 华中科技大学 | Extractive of pomegranate seeds, prepn. method and application in preparing medicine thereof |
| CN102934799A (en) * | 2012-11-16 | 2013-02-20 | 南京艾德凯腾生物医药有限责任公司 | Healthcare product containing broad bean extracts and application of healthcare product |
| CN105533686A (en) * | 2015-12-10 | 2016-05-04 | 齐鲁工业大学 | Pomegranate seed powder medicinal granules |
| CN109303333A (en) * | 2018-09-12 | 2019-02-05 | 珠海霍普金斯医药研究院股份有限公司 | A kind of Halth-care composition improving sleep |
| CN109091609A (en) * | 2018-11-01 | 2018-12-28 | 苏州卫生职业技术学院 | A kind of Chinese medicine composition and preparation method thereof with anti-oxidant blood fat reducing function |
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