CN116058494A - 一种补气固发的益生菌发酵药食同源饮料及其制备方法 - Google Patents
一种补气固发的益生菌发酵药食同源饮料及其制备方法 Download PDFInfo
- Publication number
- CN116058494A CN116058494A CN202111268694.3A CN202111268694A CN116058494A CN 116058494 A CN116058494 A CN 116058494A CN 202111268694 A CN202111268694 A CN 202111268694A CN 116058494 A CN116058494 A CN 116058494A
- Authority
- CN
- China
- Prior art keywords
- lactobacillus
- fermentation
- hair
- medicine
- food homologous
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000006041 probiotic Substances 0.000 title claims abstract description 19
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 19
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 16
- 238000002360 preparation method Methods 0.000 title abstract description 13
- 235000013361 beverage Nutrition 0.000 title abstract description 10
- 238000005728 strengthening Methods 0.000 title abstract description 7
- 238000000855 fermentation Methods 0.000 claims abstract description 38
- 230000004151 fermentation Effects 0.000 claims abstract description 38
- 239000007788 liquid Substances 0.000 claims abstract description 23
- 241000186660 Lactobacillus Species 0.000 claims abstract description 8
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 8
- 244000111489 Gardenia augusta Species 0.000 claims abstract description 6
- 241000234435 Lilium Species 0.000 claims abstract description 6
- 230000001954 sterilising effect Effects 0.000 claims abstract description 6
- 241000186840 Lactobacillus fermentum Species 0.000 claims abstract description 5
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 5
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 5
- 241000186604 Lactobacillus reuteri Species 0.000 claims abstract description 5
- 239000001963 growth medium Substances 0.000 claims abstract description 5
- 229940012969 lactobacillus fermentum Drugs 0.000 claims abstract description 5
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 5
- 229940001882 lactobacillus reuteri Drugs 0.000 claims abstract description 5
- 235000013379 molasses Nutrition 0.000 claims abstract description 5
- 241001608472 Bifidobacterium longum Species 0.000 claims abstract description 3
- 235000018958 Gardenia augusta Nutrition 0.000 claims abstract description 3
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 3
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 3
- 244000199885 Lactobacillus bulgaricus Species 0.000 claims abstract description 3
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 claims abstract description 3
- 241000218588 Lactobacillus rhamnosus Species 0.000 claims abstract description 3
- 244000057717 Streptococcus lactis Species 0.000 claims abstract description 3
- 235000014897 Streptococcus lactis Nutrition 0.000 claims abstract description 3
- 229940009291 bifidobacterium longum Drugs 0.000 claims abstract description 3
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims abstract description 3
- 229940004208 lactobacillus bulgaricus Drugs 0.000 claims abstract description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 38
- 229960002685 biotin Drugs 0.000 claims description 20
- 235000020958 biotin Nutrition 0.000 claims description 20
- 239000011616 biotin Substances 0.000 claims description 20
- 239000000203 mixture Substances 0.000 claims description 16
- 235000013305 food Nutrition 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 230000001580 bacterial effect Effects 0.000 claims description 13
- 239000000758 substrate Substances 0.000 claims description 10
- 239000008213 purified water Substances 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 6
- 239000009636 Huang Qi Substances 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 4
- 239000002609 medium Substances 0.000 claims description 3
- 238000010790 dilution Methods 0.000 claims description 2
- 239000012895 dilution Substances 0.000 claims description 2
- 238000001035 drying Methods 0.000 claims description 2
- 235000013399 edible fruits Nutrition 0.000 claims description 2
- 238000004108 freeze drying Methods 0.000 claims description 2
- 238000011081 inoculation Methods 0.000 claims description 2
- 239000000843 powder Substances 0.000 claims description 2
- 238000007873 sieving Methods 0.000 claims description 2
- 241000894007 species Species 0.000 claims description 2
- 241000699670 Mus sp. Species 0.000 abstract description 23
- 201000004384 Alopecia Diseases 0.000 abstract description 18
- 210000004209 hair Anatomy 0.000 abstract description 18
- 230000000694 effects Effects 0.000 abstract description 15
- 230000003676 hair loss Effects 0.000 abstract description 6
- 208000024963 hair loss Diseases 0.000 abstract description 5
- 241000756943 Codonopsis Species 0.000 abstract description 2
- 231100000215 acute (single dose) toxicity testing Toxicity 0.000 abstract description 2
- 238000011047 acute toxicity test Methods 0.000 abstract description 2
- 230000001939 inductive effect Effects 0.000 abstract description 2
- 231100000331 toxic Toxicity 0.000 abstract description 2
- 241001061264 Astragalus Species 0.000 abstract 1
- 230000003213 activating effect Effects 0.000 abstract 1
- 235000006533 astragalus Nutrition 0.000 abstract 1
- 230000003648 hair appearance Effects 0.000 abstract 1
- 239000011159 matrix material Substances 0.000 abstract 1
- 210000004233 talus Anatomy 0.000 abstract 1
- 230000002588 toxic effect Effects 0.000 abstract 1
- 239000008280 blood Substances 0.000 description 13
- 210000004369 blood Anatomy 0.000 description 13
- 231100000360 alopecia Toxicity 0.000 description 12
- 239000003814 drug Substances 0.000 description 10
- 239000000047 product Substances 0.000 description 7
- 241001465754 Metazoa Species 0.000 description 6
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 6
- 230000001737 promoting effect Effects 0.000 description 6
- 210000000952 spleen Anatomy 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 235000009508 confectionery Nutrition 0.000 description 5
- 230000002496 gastric effect Effects 0.000 description 5
- 210000004051 gastric juice Anatomy 0.000 description 5
- 210000004072 lung Anatomy 0.000 description 5
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 210000002784 stomach Anatomy 0.000 description 4
- 230000001502 supplementing effect Effects 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 230000000968 intestinal effect Effects 0.000 description 3
- 239000004310 lactic acid Substances 0.000 description 3
- 235000014655 lactic acid Nutrition 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 201000004624 Dermatitis Diseases 0.000 description 2
- 241000699666 Mus <mouse, genus> Species 0.000 description 2
- 206010044625 Trichorrhexis Diseases 0.000 description 2
- 108010059993 Vancomycin Proteins 0.000 description 2
- 231100000460 acute oral toxicity Toxicity 0.000 description 2
- 238000010171 animal model Methods 0.000 description 2
- 239000003833 bile salt Substances 0.000 description 2
- 235000010633 broth Nutrition 0.000 description 2
- 235000005911 diet Nutrition 0.000 description 2
- 230000037213 diet Effects 0.000 description 2
- 210000002216 heart Anatomy 0.000 description 2
- 230000003752 improving hair Effects 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 231100000572 poisoning Toxicity 0.000 description 2
- 230000000607 poisoning effect Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 230000035900 sweating Effects 0.000 description 2
- 208000011580 syndromic disease Diseases 0.000 description 2
- 229960003165 vancomycin Drugs 0.000 description 2
- MYPYJXKWCTUITO-UHFFFAOYSA-N vancomycin Natural products O1C(C(=C2)Cl)=CC=C2C(O)C(C(NC(C2=CC(O)=CC(O)=C2C=2C(O)=CC=C3C=2)C(O)=O)=O)NC(=O)C3NC(=O)C2NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(CC(C)C)NC)C(O)C(C=C3Cl)=CC=C3OC3=CC2=CC1=C3OC1OC(CO)C(O)C(O)C1OC1CC(C)(N)C(O)C(C)O1 MYPYJXKWCTUITO-UHFFFAOYSA-N 0.000 description 2
- MYPYJXKWCTUITO-LYRMYLQWSA-O vancomycin(1+) Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=C2C=C3C=C1OC1=CC=C(C=C1Cl)[C@@H](O)[C@H](C(N[C@@H](CC(N)=O)C(=O)N[C@H]3C(=O)N[C@H]1C(=O)N[C@H](C(N[C@@H](C3=CC(O)=CC(O)=C3C=3C(O)=CC=C1C=3)C([O-])=O)=O)[C@H](O)C1=CC=C(C(=C1)Cl)O2)=O)NC(=O)[C@@H](CC(C)C)[NH2+]C)[C@H]1C[C@](C)([NH3+])[C@H](O)[C@H](C)O1 MYPYJXKWCTUITO-LYRMYLQWSA-O 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 1
- 238000009631 Broth culture Methods 0.000 description 1
- 241000007126 Codonopsis pilosula Species 0.000 description 1
- 208000036649 Dysbacteriosis Diseases 0.000 description 1
- 208000027244 Dysbiosis Diseases 0.000 description 1
- 206010013954 Dysphoria Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- ZFMITUMMTDLWHR-UHFFFAOYSA-N Minoxidil Chemical compound NC1=[N+]([O-])C(N)=CC(N2CCCCC2)=N1 ZFMITUMMTDLWHR-UHFFFAOYSA-N 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- 208000001431 Psychomotor Agitation Diseases 0.000 description 1
- 206010038743 Restlessness Diseases 0.000 description 1
- 208000013738 Sleep Initiation and Maintenance disease Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 231100000403 acute toxicity Toxicity 0.000 description 1
- 230000007059 acute toxicity Effects 0.000 description 1
- 229940107666 astragalus root Drugs 0.000 description 1
- 208000010668 atopic eczema Diseases 0.000 description 1
- 230000037358 bacterial metabolism Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 150000001615 biotins Chemical class 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000007140 dysbiosis Effects 0.000 description 1
- 230000008482 dysregulation Effects 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000021107 fermented food Nutrition 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000020510 functional beverage Nutrition 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 238000003304 gavage Methods 0.000 description 1
- 210000002149 gonad Anatomy 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 244000005709 gut microbiome Species 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 206010022437 insomnia Diseases 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000027939 micturition Effects 0.000 description 1
- 229960003632 minoxidil Drugs 0.000 description 1
- 239000000820 nonprescription drug Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 230000001047 pyretic effect Effects 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000002453 shampoo Substances 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 238000010025 steaming Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 210000000106 sweat gland Anatomy 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 230000003813 thin hair Effects 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
- A23L2/382—Other non-alcoholic beverages fermented
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
本发明公开了一种具有补气固发的益生菌发酵药食同源饮料及其制备方法,所述药食同源的比例为:栀子3份,百合6份,黄芪1份,党参1份;益生菌发酵的菌种为:鼠李糖乳杆菌、植物乳杆菌、格式乳杆菌、罗伊氏乳杆菌、长双歧杆菌、发酵乳杆菌、乳酸乳球菌、嗜酸乳杆菌、保加利亚乳杆菌中的一种或多种。益生菌菌种经甘蔗糖蜜培养基活化后接种至灭菌药食同源基质中,液态发酵24小时后滤渣即获得一种补气固发的益生菌发酵药食同源饮料。该饮料通过急毒试验验证无任何毒副作用,通过脱发小鼠诱导重新生发,说明本发明物安全可食用,且具有诱导重新生发,改善发质的作用。
Description
技术领域
本发明涉及一种食品,具体涉及一种补气固发的乳酸菌发酵药食同源饮料及其制备方法,属于功能饮料制备和应用领域。
背景技术
脱发是困扰中年人的生活难题之一,人群庞大,市场上缓解脱发的产品种类繁多,以米诺地尔类OTC产品为龙头,其次为药食同源洗发露,种类繁多,市场竞争激烈。这部分兴起的产品大多为外用产品,使用后效果反复,易反弹,不能从根本上解决脱发问题,最新有研究指出肠道菌群与脱发有着密切关系,如Cell Reports指出小鼠肠道内菌群合成生物素可有效改善发质,防止头发断裂,促进脱发小鼠重新生长出毛发。所以调整肠道菌群平衡,提高体内生物素含量能够在治疗脱发的过程中发挥作用。
中医学认为脱发的原因有两点:一是血热风燥说,《儒门事亲》说:“年少早白落,此血热太过也。世俗只知发者血之余,血衰故耳。岂知血热而发反不茂肝者,木也。火多水少,木反不荣;火至于顶,炎上之甚也。热病汗后,发多脱落,岂有寒耶。”血为水谷精微所化,以奉养周身。血热生风,风热随气上窜于巅顶,毛根得不到阴血的滋养,头发则会突然脱落或焦黄或早白等。二是脾胃湿热说,《临证指南》说:“湿从内生者,必其人膏粱酒鳢过度。”说明恣食肥甘,容易伤胃损脾,湿热内蕴,发根循经上蒸巅顶,侵蚀发根白浆,导致头发粘腻,头发稀少或者均匀性脱发。
百合性甘、微寒,归肺、心经;有润肺养阴、失眠多梦、热病后的余热未清、虚烦不安等功效。栀子性苦、寒,归心、肝、肺、胃、三焦经;有泻火除烦、凉血止血解毒、清利湿热、肝经湿热郁火等功效。党参性甘、平,归脾、肺经;有补中益气、养血补津等功效。黄芪性甘、微温,归脾、肺经;有补气健脾升阳、益卫固表止汗、利尿,托疮生肌等功效。生物素有促进汗腺、神经组织、骨髓、男性性腺、皮肤及毛发的正常运作和生长、减轻湿疹、皮炎症状、预防白发及脱发、有助于治疗秃顶等功效。
发明内容
本发明的目的是提供一种乳酸菌发酵药食同源饮料,以改善现在人群气亏导致严重脱发的技术问题。
本发明主要是通过以下技术方案解决上述技术问题的:一种益生菌发酵药食同源饮料,包括乳酸菌菌种、栀子、百合、黄芪、党参,所述益生菌菌种为高产生物素乳酸菌,栀子等药食同源为中医治疗脱发的成分。
一种补气固发的乳酸菌发酵药食同源组合物的饮料及其制备方法,包括以下步骤:
步骤A:发酵种子液制备
将高产生物素菌株分别按2%分别接种于甘蔗糖蜜培养基中,置于37℃静置培养24小时,获得发酵种子液。
步骤B:发酵基质制备
将药食同源组合物转移至发酵罐中,加入纯化水后使用纯蒸汽进行115℃,30分钟杀菌,冷却至常温后获得发酵基质。
步骤C:将步骤1制备发酵种子液按比例接种至步骤2制备发酵基质中,恒温搅拌发酵,发酵产物为一种补气固发益生菌发酵药食同源组合物饮料。
本发明具有以下优点:
1. 本发明口味独特、食用健康、有效预防脱发;
2. 发酵菌种能够高产生物素,药食同源组合物具有祛火生血的功能;
3. 发酵后活菌含量高达108cfu/mL。
附图说明
附图用来提供对本发明的进一步理解,并且构成说明书的一部分,与本发明的实施一起用于解释本发明,并不构成对本发明的限制。
附图1为脱发小鼠服用本发明物后诱导重新生发的机制。
具体实施方式
下面对本发明实施例中的技术方案进行清楚、完整地描述,显然,所描述的实施例仅仅是本发明一部分实施例,而不是全部的实施例。
本案例的实施物料,检测方法,均符合国家相关标准要求。
实施例1:筛选高产生物素菌株
恒康生物微生物菌种保藏中心(HKMCC)现保存81株乳酸菌收录于中国可用于食品的菌种名单。从此名单中初步筛选抗逆性强的12株乳酸菌进一步进行高产生物素菌株的筛选工作,具体如下:
步骤A:强抗逆性菌株筛选
(1)溶液的配制
pH1.5人工胃液:参考中国药典的人工胃液配方:取稀盐酸16.4mL,加水约800mL及胃蛋白酶10g,搅匀后加水定容至1000mL,0.22um水系滤器过滤除菌即可。(稀盐酸为234mL浓盐酸加水稀释至1000mL)其余pH的人工胃液直接在pH1.5的人工胃液下调节pH即可。
3g/L的牛胆盐:在液体培养基中加入牛胆盐,使之浓度达到3g/L,高压灭菌。
(2)抗逆性检测
选取菌种活化24小时,取菌液5000r/min离心20min后加入等体积人工胃液和3g/L胆盐,置于37℃处理2h,对未处理的菌液及处理2h后的菌液进行活菌计数计算存活率(如附表1所示)。
步骤B:高产生物素菌株筛选
(1)待测菌液制备
从超低温冰箱中将步骤A筛选的菌种取出,待常温解冻后按2%比例接种于MRS肉汤培养基,37±1℃培养19~24小时后获得一级种子液。从一级种子液中取菌液按2%比例重新接种培养后获得二级种子液。由于菌种在冰箱保存时间不一致可能导致活性差异,故使用二级种子液按2%比例接种至MRS肉汤培养24小时获得待测菌液以减小菌种活性造成的差异。
(2)无菌发酵液的制备
将待检测菌液置于离心机以10000 rpm离心10分钟后取上清液,在无菌操作台中用0.22μm水系滤头过滤离心上清液,滤液为无菌发酵液。
(3)生物素含量检测
具体检测步骤参照GB 5009.259-2016 《食品安全国家标准 食品中生物素的测定》进行修改制定。
表1 乳酸菌抗逆性及产生物素含量
实施例2:益生菌发酵药食同源组合物
步骤A:发酵种子液制备
将高产生物素菌种分别按2%分别接种于甘蔗糖蜜培养基中,置于37℃静置培养24小时,获得发酵种子液。
作为优选,所述益生菌菌种包括鼠李糖乳杆菌、植物乳杆菌、格式乳杆菌、罗伊氏乳杆菌、长双歧杆菌、发酵乳杆菌、乳酸乳球菌、嗜酸乳杆菌、保加利亚乳杆菌中的一种或多种。更优选地,所述益生菌菌种包括植物乳杆菌、罗伊氏乳杆菌、发酵乳杆菌。
作为优选,所述甘蔗糖蜜培养基使用纯化水稀释至糖度为(2~10)Brix,使用115℃高压蒸汽灭菌30分钟。更优选地,所述稀释至糖度为(4.9~5.5)Brix。
步骤B:发酵基质制备
将药食同源组合物转移至发酵罐中,加入纯化水后使用纯蒸汽进行115℃,30分钟杀菌,冷却至常温后获得发酵基质。
作为优选,所述药食同源组合物为干燥粉碎后过50~200目筛的粉剂,栀子:百合:黄芪:党参的混合比例为(1~6):(1~6):(1~6):(1~6)。更优选地,所述干燥方式为冷冻干燥,粉碎后过100目筛,栀子:百合:黄芪:党参的混合比例为3:6:1:1。
作为优选,所述发酵基质为1质量份药食同源组合物加入(10~30)质量份纯化水混合。更优选为1质量份药食同源组合物加入19质量份纯化水混合。
步骤C:将步骤A制备发酵种子液按比例接种至步骤B制备发酵基质中,恒温搅拌发酵,发酵产物为一种补气固发益生菌发酵药食同源组合物饮料。
作为优选,所述发酵种子液的接种比例为1‰~20‰。
作为优选,所述恒温搅拌发酵为控温37℃,转速30rpm,发酵时间24小时。
实验例1:益生菌发酵药食同源组合物急毒试验
本实验主要针对本发明物的急毒效果通过小鼠灌胃试验进行测定;通过对雌、雄小鼠给以10000mg/kg·bw,连续观察14天,记录体重变化和死亡情况判断急毒效果。
实验对象为健康成年SPF级ICR小鼠20只,雌雄各半,由南京医科大学医药实验动物中心提供,生产许可证号SCXK(苏)2016-0002,合格证编号:NYD-L-2020110905。实验动物在温度为20~26℃、相对湿度为40%~70%的本中心屏障***中饲养,实验动物使用许可证号:SYXK(苏)2020-0006。实验鼠维持饲料(Co60辐照)由江苏省协同医药生物工程有限责任公司提供,生产许可证:苏饲证(2019)01008。
采用限量法,设定剂量为10000 mg/kg•bw,每组10只动物,雌雄各半。动物检疫3d,试验时雌性体重为19.2~21.0g,雄性体重为19.9~22.3g,试验前动物禁食4h,自由饮水。样品比重1.008,以原液按10 mL/kg•bw,1次灌胃给样,实际剂量为10080 mg/kg•bw。全部动物连续观察14天,记录中毒表现及死亡情况。
灌胃给予ICR小鼠10080 mg/kg•bw本发明物。雌、雄性动物14d试验观察期间,饮食和活动正常,生长良好,未见中毒表现及异常体征,无动物死亡(见表1)。所以,本发明物的雌、雄性小鼠急性经口毒性试验LD50均为大于10080 mg/kg•bw,属实际无毒(结果如表2所示)。
表2 小鼠急性经口毒性试验结果
实验例2:益生菌发酵药食同源组合物促进小鼠重新生发
最近有研究表明,脱发的发生与肠道微生物菌群失调存在关联,而生物素是一种高度依赖细菌代谢产生的营养素,生物素的缺乏会导致头发断裂和严重的皮肤疾病。本发明物旨在通过补充高产生物素的益生菌以缓解由于肠道菌群失调引起的脱发同时,补充生物素改善发质。研究主要通过以下方案验证:
选取健康成年SPF级雌雄小鼠各20只,按照性别随机分成两组,每组10只雌性小鼠,10只雄性小鼠。使用万古霉素分别处理4周后,观察到所有小鼠背部毛发脱落,从各组随机取血液和粪便样品进行生物素含量测定。在保证正常供水给食的基础上停用万古霉素,A组小鼠使用本发明物灌胃处理,灌胃量为20mL/d·kg,B组小鼠使用生理盐水替换进行灌胃处理,灌胃量为20mL/d·kg。持续给药8周后,按0~10分对小鼠毛发进行视觉评分,将小鼠背部毛发刮下来后称重,取血液和粪便样品测定生物素含量(结果如表3所示)。
附表3 益生菌发酵药食同源饮料对小鼠生理指标影响
恢复饮食后,小鼠逐渐恢复生物素水平,给予本发明物后,小鼠体内生物素含量显著提高,重新生长毛发。同时发现本发明物对于雄性小鼠的重新生发效果明显优于雌性,可能针对男性的诱导重新生发效果优于女性。
Claims (7)
1.将高产生物素菌种分别按2%分别接种于甘蔗糖蜜培养基中,置于37℃静置培养24小时,获得发酵种子液。
2.权利要求1所述高产生物素菌种包括鼠李糖乳杆菌、植物乳杆菌、格式乳杆菌、罗伊氏乳杆菌、长双歧杆菌、发酵乳杆菌、乳酸乳球菌、嗜酸乳杆菌、保加利亚乳杆菌中的一种或多种。更优选地,所述益生菌菌种包括植物乳杆菌、罗伊氏乳杆菌、发酵乳杆菌。
3.权利要求1所述甘蔗糖蜜培养基使用纯化水稀释至糖度为(2~10)Brix,使用115℃高压蒸汽灭菌30分钟。更优选地,所述稀释至糖度为(4.9~5.5)Brix。
4.将药食同源组合物转移至发酵罐中,加入纯化水后使用纯蒸汽进行115℃,30分钟杀菌,冷却至常温后获得发酵基质。
5.权利要求4所述药食同源组合物为干燥粉碎后过50~200目筛的粉剂,栀子:百合:黄芪:党参的混合比例为(1~6):(1~6):(1~6):(1~6)。更优选地,所述干燥方式为冷冻干燥,粉碎后过100目筛,栀子:百合:黄芪:党参的混合比例为3:6:1:1。
6.权利要求4所述发酵基质为1质量份药食同源组合物加入(10~30)质量份纯化水混合。更优选为1质量份药食同源组合物加入19质量份纯化水混合。
7.将权利要求1制备发酵种子液按比例接种至权利要求4制备发酵基质中,恒温搅拌发酵。所述发酵种子液的接种比例为1‰~20‰,所述恒温搅拌发酵为控温37℃,转速30 rpm,发酵时间24小时。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202111268694.3A CN116058494A (zh) | 2021-10-29 | 2021-10-29 | 一种补气固发的益生菌发酵药食同源饮料及其制备方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202111268694.3A CN116058494A (zh) | 2021-10-29 | 2021-10-29 | 一种补气固发的益生菌发酵药食同源饮料及其制备方法 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN116058494A true CN116058494A (zh) | 2023-05-05 |
Family
ID=86182350
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202111268694.3A Pending CN116058494A (zh) | 2021-10-29 | 2021-10-29 | 一种补气固发的益生菌发酵药食同源饮料及其制备方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN116058494A (zh) |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20160070508A (ko) * | 2014-12-10 | 2016-06-20 | (주)하이모 | 탈모예방 및 발모촉진 효능을 갖는 발효 효소 식품 |
KR20170100828A (ko) * | 2016-02-26 | 2017-09-05 | 농업회사법인 우리엘바이오 주식회사 | 탈모 방지 조성물 및 그 제조방법 |
CN108041385A (zh) * | 2017-12-28 | 2018-05-18 | 上海交大昂立股份有限公司 | 一种复合益生菌药食同源植物发酵饮料及其制备方法 |
-
2021
- 2021-10-29 CN CN202111268694.3A patent/CN116058494A/zh active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20160070508A (ko) * | 2014-12-10 | 2016-06-20 | (주)하이모 | 탈모예방 및 발모촉진 효능을 갖는 발효 효소 식품 |
KR20170100828A (ko) * | 2016-02-26 | 2017-09-05 | 농업회사법인 우리엘바이오 주식회사 | 탈모 방지 조성물 및 그 제조방법 |
CN108041385A (zh) * | 2017-12-28 | 2018-05-18 | 上海交大昂立股份有限公司 | 一种复合益生菌药食同源植物发酵饮料及其制备方法 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103109930B (zh) | 一种添加丝胶抗冻肽的果味益生菌酸奶片及其制备方法 | |
CN105054040B (zh) | 一种益生菌发酵人参的组合物及其制备方法和应用 | |
CN107581436B (zh) | 一种益生菌发酵枸杞浓浆的制备方法 | |
JP2010095465A (ja) | 乳酸菌含有免疫賦活用組成物 | |
CN105146614A (zh) | 一种功能性钙果酵素、酵素饮料及其生产方法 | |
KR20060135016A (ko) | 유카 추출물, 퀼라야 추출물 및 유산균을 포함하는 조성물및 상기 조성물을 함유하는 음식물 | |
CN106754619A (zh) | 一种在谷物培养基中添加中药组分促进益生菌生长的方法 | |
CN104542977A (zh) | 一种含山药提取物和两歧双歧杆菌的保健饮品及制备方法 | |
CN104489092A (zh) | 一种含罗汉果提取物和活性长双歧杆菌的保健饮品及其制备方法 | |
CN113755409B (zh) | 一种缓解胰岛素抵抗的长双歧杆菌及其应用 | |
CN109380733A (zh) | 一种益生菌粉剂及其制备方法 | |
KR20040027180A (ko) | 비만 또는 당뇨병의 예방 및 치료 효과를 갖는 유산균발효 유제품 및 그 제조방법 | |
CN113604395A (zh) | 一株可发酵石斛且其发酵液可改善皮肤质量的植物乳杆菌 | |
CN110959867A (zh) | 母乳化的复合益生菌微胶囊粉及其制备方法和应用 | |
CN106858577A (zh) | 制备具有保肝护肝、调节脂肪肝代谢功能的植物酵素的发酵组合物及制法 | |
EP2262514B1 (en) | Agents for promoting secretion and/or suppressing decrease of adiponectin | |
CN114504623A (zh) | 改善气虚型便秘的中药益生菌复合制品及其制备方法 | |
CN114657084B (zh) | 一种缓解溃疡性结肠炎的长双歧杆菌及其应用 | |
CN111387383A (zh) | 一种具有减肥降脂功效的高多酚含量益生菌发酵苹果汁及制备方法 | |
CN114214230A (zh) | 一株具有共聚幽门螺杆菌能力的北酸乳杆菌及其应用 | |
CN112626001B (zh) | 促进丁酸菌生长的益生质组合物及其用途 | |
CN113208077A (zh) | 一种益生菌枸杞醋果冻的制备方法 | |
US20230346856A1 (en) | Bifidobacterium brevis and its application in preventing or alleviating psoriasis thereof | |
JP6261688B2 (ja) | Qol改善又は持続剤 | |
JP2003252770A (ja) | 糖尿病合併症予防・改善・治療剤 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination |