CN116026910A

CN116026910A - Novel method for detecting helicobacter pylori

Info

Publication number: CN116026910A
Application number: CN202111251759.3A
Authority: CN
Inventors: 黄卫; 李海洋; 王卫国; 李京华; 仓怀文
Original assignee: Dalian Institute of Chemical Physics of CAS
Current assignee: Dalian Institute of Chemical Physics of CAS
Priority date: 2021-10-26
Filing date: 2021-10-26
Publication date: 2023-04-28

Abstract

The invention provides a novel method for detecting helicobacter pylori, which comprises the following steps: the non-radioactive urea capsule is taken orally by a subject, and after sitting still for 10-20 minutes, the subject blows air into an expiration sampling bag, and an expiration air sample of the subject is reserved; then, detecting NH in the breath sampling bag by using an ion mobility spectrometer ₃ Concentration level of NH in the exhaled breath of normal person ₃ Is compared with the concentration level of (a); if the detected concentration is significantly higher than the normal level, it is diagnosed as a helicobacter pylori infected person. The method provided by the invention does not need to be eaten ¹³ C and C ¹⁴ The urea of C has no radioactivity, basically has no side effect on human bodies, is suitable for diagnosis of sensitive people such as the old, children, pregnant women and the like, and has higher diagnosis sensitivity and shorter diagnosis time.

Description

Novel method for detecting helicobacter pylori

Technical Field

The invention relates to a novel method for detecting helicobacter pylori.

Background

Infection with Helicobacter Pylori (HP) is a major cause of chronic gastritis, which is closely related not only to peptic ulcers but also to one of the origins of gastric cancer. Early treatment was found to be an important means of suppressing exacerbation of helicobacter pylori infection.

At present, the means for detecting helicobacter pylori infection can be mainly divided into two types: one is an invasive method; another type is a non-invasive method.

Invasive methods are mainly those using gastroscopy and gastric mucosa biopsy. Despite the high accuracy, this approach is relatively exclusive to most patients and requires high levels of medical personnel, high costs, and is not conducive to screening, etc.

Non-invasive methods are largely divided into two types, serum detection and isotope labeling. The serum mode is to detect HP antibodies in blood. Since the patient has the humor bacteria antibody in blood for one or two years after healing, false positives are likely to be detected, and the patient is rarely used in hospitals at present.

At present, a hospital generally adopts an isotope tracer mode machine for detection, namely Urea expiration Test (UBT). By taking the buccal tablet ¹³ C and C ¹⁴ C urea is decomposed into CO by urease in helicobacter pylori ₂ And NH ₃ By detecting ¹³ CO ₂ And ¹⁴ CO ₂ and (5) detecting the humor indirectly. But is provided with ¹⁴ C has radioactivity, pollutes human body and environment, and is not suitable for pregnant women and children. ¹³ C is ¹⁴ C upgrade, sensitivity and specificity are higher. The method is greatly improved compared with an invasive method, but has certain limitations, such as complicated extraction and preparation processes of isotope medicament and high medicament cost; CO ₂ The content of the medicine in the exhaled air of the human body is higher, the dosage of the medicine required for causing obvious pathological changes is more, the reaction time is longer, and the like.

Thus, there is still a need to develop a non-invasive, portable, low cost, convenient-to-screening technique and apparatus for detecting helicobacter pylori infection.

Ion mobility spectrometry (Ion Mobility Spectrometry, IMS) is a technique for separating and detecting sample ions by a uniform weak electric field according to the difference in ion mobility at atmospheric pressure. The method has the advantages of high detection speed (single detection period is less than 20 ms), high sensitivity, easy realization of miniaturization, low cost and the like, and is widely applied to the fields of military, environmental monitoring and the like.

In the early stage, we have developed a high-precision NH measurement based on ion mobility spectrometry technology ₃ (Long-term sub second-response monitoring of gaseous ammonia in ambient air by positive inhaling ion mobility spectrometry, talanta,175, 522-527).

To this end, the invention discloses a novel method for detecting helicobacter pylori. The method is carried out by taking conventional urea (different from ¹³ C and C ¹⁴ Urea of C) and then detecting NH in the exhaled breath by ion mobility spectrometry ₃ Is diagnostic for helicobacter pylori infection.

Disclosure of Invention

The invention provides a novel method for detecting helicobacter pylori, which comprises the following specific technical scheme:

the subject takes a non-radioactive urea capsule (distinguished from warm boiled water ¹³ C and C ¹⁴ C, urea) and after sitting for 10-20 minutes, blowing air into a special expiration sampling bag, and reserving an expiration gas sample of the testee. Then, detecting NH in the breath sampling bag by using an ion mobility spectrometer ₃ Concentration level of NH in the exhaled breath of normal person ₃ Is compared to the concentration level of (c). If the detected concentration is significantly higher than the normal level, it is diagnosed as a helicobacter pylori infected person.

The detection principle is as follows: helicobacter pylori contains a large amount of helicobacter pylori urease. Urea is decomposed into CO under the action of helicobacter pylori urease ₂ And NH ₃ . Then by detecting NH in exhaled breath ₃ Is diagnostic for helicobacter pylori infection.

NH ₂ CONH ₂ →CO ₂ +NH ₃

Based on the scheme, the oral dosage of the urea capsule is preferably 50-200 mg, and the urea capsule is orally taken by warm boiled water.

Based on the above scheme, preferably, after taking urea, normal people exhale NH in the air ₃ The concentration level of (2) is less than 0.8ppm; in the case of helicobacter pylori infection, NH is present in exhaled breath ₃ May be higher than 2ppm.

Based on the above scheme, preferably, the sampling mode is: the connecting tube is used to connect the mouthpiece with the sampling bag, and then the subject blows air into the sampling bag using the mouthpiece. The connecting pipe is made of a tetrafluoro material, and the sampling bag is a gas sampling bag with a tetrafluoro material lining.

Based on the above, it is preferable that the ion mobility spectrometer employs a non-radioactive ionization source, using ketones as dopants. Such as butanone.

Based on the above scheme, preferably, the urea contained in the urea capsule is common edible urea.

The invention has the following advantages:

the method of the invention does not need to be eaten ¹³ C and C ¹⁴ The urea of C has no radioactivity to human body and no side effect to human body, and is suitable for diagnosis of sensitive people such as old people, children, pregnant women, etc.

The method of the invention has higher diagnosis sensitivity. CO in exhaled air of human body ₂ Is used in the concentration of 40000ppm, ¹³ the abundance of C is 1.1%, ¹³ CO ₂ is about 400ppm. While in the exhaled breath NH ₃ The background concentration of (2) is about 0.8ppm, well below ¹³ CO ₂ Background concentration of (2). Thus, low dose NH ₃ The change of concentration can be used for diagnosing helicobacter pylori infection.

The method has short diagnosis time. The single diagnosis time is less than 20min, the detection report is immediately available, and the method is suitable for general investigation of large-flux people.

The method has the advantages of small volume, light weight, portability and easy operation.

Drawings

FIG. 1 is a spectral peak of clean air;

FIG. 2 is a spectral peak of exhaled breath of the subject of example 1 after administration of urea capsules;

FIG. 3 NH in exhaled breath ₃ Is a quantitative curve of (a).

Detailed Description

The following examples illustrate the use of the invention, but do not limit the scope of application. In the following examples, ion mobility spectrometer detection of NH ₃ Reference is made to Long-term sub second-response monitoring of gaseous ammonia in ambient air by positive inhaling ion mobility spectrometry, talanta,175, 522-527.

Example 1

The testee takes a urea capsule with warm boiled water completely, and the dosage of the urea capsule is 100mg; after sitting for 10-20 minutes, the special exhalation sampling bag is blown, and a sample of the exhaled air of the testee is left. Then, detecting NH in the breath sampling bag by using an ion mobility spectrometer ₃ Concentration level of NH in the exhaled breath of normal person ₃ Is compared to the concentration level of (c). Wherein, the ion mobility spectrometer adopts a non-radioactive ionization source and uses butanone as a doping agent. The detection result is shown in figure 2, and the exhaled gas NH is quantified by the curve shown in figure 3 ₃ The concentration was 0.4ppm, and it was determined from the detection result that the subject was a helicobacter pylori infected subject.

Claims

1. A novel method for detecting helicobacter pylori, characterized by: the method comprises the following steps: the non-radioactive urea capsule is taken orally by a subject, and after sitting still for 10-20 minutes, the subject blows air into an expiration sampling bag, and an expiration air sample of the subject is reserved; then, detecting NH in the breath sampling bag by using an ion mobility spectrometer ₃ Concentration level of NH in the exhaled breath of normal person ₃ Is compared with the concentration level of (a); if the detected concentration is significantly higher than the normal level, it is diagnosed as a helicobacter pylori infected person.

2. The novel method for detecting helicobacter pylori according to claim 1, characterized in that: the urea contained in the urea capsule is common edible urea.

3. The novel method for detecting helicobacter pylori according to claim 1, characterized in that: the oral dosage of the urea capsule is 50-200 mg, and the urea capsule is orally taken by warm boiled water.

4. The novel method for detecting helicobacter pylori according to claim 1, characterized in that: after taking urea, NH in the exhaled air of normal person ₃ Is less than 0.8ppm; in the case of helicobacter pylori infection, NH is present in exhaled breath ₃ The concentration level of (2) is 2ppm or more.

5. The novel method for detecting helicobacter pylori according to claim 1, characterized in that: the ion mobility spectrometer employs a non-radioactive ionization source, using ketones as dopants.

6. The novel method for detecting helicobacter pylori according to claim 1, characterized in that: during sampling, a connecting pipe is used for connecting the blowing nozzle and the sampling bag, and then a subject blows air into the air sampling bag by using the blowing nozzle; wherein, the connecting pipe is the tetrafluoro material, and the inside lining of the gas sampling bag is the tetrafluoro material.