CN116004098A - Inner wall coating of waterway pipeline of stomatology treatment table, and preparation method and application thereof - Google Patents
Inner wall coating of waterway pipeline of stomatology treatment table, and preparation method and application thereof Download PDFInfo
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- CN116004098A CN116004098A CN202310024427.4A CN202310024427A CN116004098A CN 116004098 A CN116004098 A CN 116004098A CN 202310024427 A CN202310024427 A CN 202310024427A CN 116004098 A CN116004098 A CN 116004098A
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Abstract
The invention relates to an inner wall coating of a waterway pipeline of an stomatology treatment table, a preparation method and application thereof, wherein the inner wall coating comprises PCL and ZnO, and the weight ratio of the ZnO to the PCL is 20-100%. The preparation method comprises the following steps: step one, utilize CH 2 Cl 2 Dissolving PCL to obtain PCL solution; step two, adding ZnO powder into the PCL solution, and uniformly stirring to obtain a mixed solution; and thirdly, soaking the PU in the mixed solution, slowly pulling out, and drying. The inner wall coating provided by the invention utilizes the antibacterial effect of ZnO releasing Zn ions and the hydrophobicity of PCL, and can inhibit bacterial adhesion in early stage and inhibit bacterial proliferation in late stage, thereby realizing stomatology treatmentLong-acting antibacterial function for waterway pipeline of therapeutic table.
Description
Technical Field
The invention belongs to the field of biomedical materials, and particularly relates to an inner wall coating of a waterway pipeline of an oral treatment table, a preparation method and application thereof, which can be applied to long-acting antibiosis of the waterway pipeline of the oral treatment table.
Background
The comprehensive oral treatment table is a medical device for dental treatment, and is suitable for examination, diagnosis and treatment of various dental diseases. The water path of the oral cavity comprehensive treatment table is a complex set of pore pipelines which are connected with each other, and supplies water to all rinsing and cooling devices, cup water filling ports, bowl-shaped rinsing outlets and the like which are connected with the water path, such as devices of a conventional dental handpiece, a high-speed turbine dental handpiece, a three-purpose gun, an ultrasonic dental scaler and the like. The waterway of the oral cavity comprehensive treatment table is easy to be polluted in the use process, and the pipelines of the waterway can form biological films due to untimely and incomplete cleaning, so that a pollution source for continuously polluting treatment water is formed, and the waterway becomes a potential risk factor of nosocomial infection.
It has been reported that bacteria in the water supply pipeline can reach 2×10 within five days after the new oral treatment chair is connected with the water supply pipeline 5 At CFU/mL level, more than 30 pathogenic microorganisms can be detected in the formed biological film in the comprehensive treatment table, and the concentration of the water outlet bacteria of the water way of the oral comprehensive treatment table is as high as 10 6 CFU/mL. Most microorganisms in the water outlet of the treatment table waterway are low-pathogenicity gram-negative aerobic heterotrophic bacteria. The appearance type and range of the environmental bacteria are irregular, and are generally closely related to the layout setting, diagnosis and treatment flow and the like of the stomatology. Fungi, tissue-dissolving amoeba and saccharomycetes are also detected in the waterway of the treatment table. Most of the separated bacteria in the waterway of the treatment table are conditional pathogenic bacteria, but reports of detecting pathogenic bacteria are also provided, and the waterway mainly comprises: pseudomonas aeruginosa, legionella pneumophila and some non-tubercular bacilli.
As the water channel pollution of the oral cavity comprehensive treatment table is increasingly serious, the risk of causing the oral cavity hospital infection is increasingly increased, and research and the reason exploration of the water channel pollution of the oral cavity comprehensive treatment table also gradually draw importance to students at home and abroad. The pollution of the waterway of the currently accepted oral comprehensive treatment table mainly comes from three aspects: (1) The shape of the pipeline is various, the interfaces are various, the pressure is unstable, the diameter of the pipeline is small, the liquid flow is slow, and the liquid stops intermittently, so that bacteria are easy to adhere to the inner side of the lumen and a biological film is formed; (2) The pollution of the water source causes that the quality of the water before entering the treatment table is difficult to ensure; (3) In the using process of the dental handpiece, the dental drill contacts with blood and saliva of a patient, so that not only is the surface of the handpiece polluted, but also the dental blood, saliva and the like possibly containing pathogenic microorganisms are sucked back into a cooling water pipeline of the handpiece due to suck-back. Aiming at the waterway pollution of the comprehensive treatment table caused by the factors, researches show that after treatment is finished, the idling mobile phone 20-30s can remove as much bacteria sucked back into the mobile phone and the comprehensive treatment table as possible in a short time, meanwhile, the waterway is regularly cleaned and disinfected, bacterial pollution is removed, an independent water supply system is arranged if necessary, and the water supply is treated by physical filtration, chemical disinfection and other methods, so that the hospital infection control quality of the oral cavity diagnosis and treatment water can be improved. However, even if the dental handpiece and the water supply source are effectively disinfected, the biofilm formed by bacteria on the inner wall of the waterway pipeline of the treatment table still exists, and the conventional waterway pipeline cleaning and disinfecting can not effectively remove the biofilm on the inner wall of the waterway pipeline of the treatment table, so that the waterway pollution problem of the treatment table exists for a long time. In addition, the disinfectant is adopted to disinfect the waterway pipeline, so that certain erosion is caused to the waterway pipeline, and the service life of the waterway pipeline is reduced.
Therefore, it is a power of those skilled in the art that improvements to the inner wall of the waterway tubing of the treatment table are necessary.
Disclosure of Invention
Aiming at the problems, the invention aims to provide an inner wall coating composition of a waterway pipeline of an stomatology treatment table, a preparation method and application thereof, which can effectively inhibit the adhesion of bacteria, prevent the formation of a biological film and reduce the erosion damage of a disinfectant to the waterway pipeline.
In a first aspect, the invention provides an inner wall coating composition for a waterway pipeline of an oral treatment table, comprising PCL (polycaprolactone) and ZnO, wherein the weight ratio of ZnO to PCL is 20-100%.
Preferably, the 20-100% range includes any particular point value attributed thereto, such as 20%, 21%, 25%, 27%, 30%, 33%, 35%, 38%, 40%, 42%, 45%, 47%, 50%, 52%, 55%, 57%, 58%, 60%, 62%, 65%, 68%, 70%, 72%, 73%, 75%, 77%, 80%, 82%, 85%, 86%, 88%, 90%, 92%, 95%, 98%, 99%, 100%, and ranges of any two of these particular point values, such as 40-80%, 50-70%, or 30-90%.
In a second aspect, the invention provides a preparation method of the coating composition for the inner wall of the waterway pipeline of the dental treatment table, which comprises the following steps:
step one, utilize CH 2 Cl 2 Dissolving PCL to obtain PCL solution;
step two, adding ZnO powder into the PCL solution, and uniformly stirring to obtain a mixed solution;
and thirdly, soaking PU (polyurethane) in the mixed solution, slowly pulling out, and drying to obtain the inner wall coating composition.
Preferably, in step one, CH is used at room temperature 2 Cl 2 Dissolve PCL.
Preferably, in the third step, the specific drying process is as follows: and the mixture is placed in an oven at 60 ℃ for 12 hours.
In a third aspect, the invention also provides an application of the inner wall coating composition of the waterway pipeline of the dental treatment table in antibiosis, in particular to an application of the waterway pipeline of the dental treatment table in antibiosis.
Compared with the prior art, the invention has the following beneficial effects:
(1) When the inner wall coating is used for culturing staphylococcus aureus and escherichia coli, early adhesion (2 h) and long-term proliferation (24 h) of the staphylococcus aureus and escherichia coli can be inhibited, and the inner wall coating has good antibacterial effect.
(2) When the inner wall coating provided by the invention is used for culturing the mouse fibroblast, no obvious cytotoxicity exists, and the biocompatibility is good.
(3) The inner wall coating provided by the invention can inhibit bacterial adhesion on the surface of the PU waterway pipeline through the constructed hydrophobic coating.
(4) The inner wall coating provided by the invention can realize the long-acting antibacterial effect of the surface of the PU waterway pipeline through released Zn ions. The inorganic biological material ZnO is degraded to release bioactive Zn ions, and the Zn ions have remarkable antibacterial effect and good biocompatibility.
(5) The preparation method provided by the invention is simple and easy to implement, wide in raw material sources and low in cost.
(6) The inner wall coating provided by the invention can inhibit bacterial adhesion and proliferation of waterway pipelines of the dental treatment table, prevent the formation of biological films, reduce erosion and damage of disinfectants to the waterway pipelines, and has important application value in thoroughly solving the waterway pollution problem of the treatment table.
Drawings
FIG. 1 is a microscopic image of the PU inner wall coating material prepared in example 1 under a scanning electron microscope, which can smoothly prepare a uniform and stable surface coating material;
fig. 2 is an XRD pattern of the inner wall coating material prepared in example 1, confirming successful loading of ZnO into PU surface coating material.
Fig. 3 is a contact angle experimental result of the inner wall coating material prepared in example 1, each group of coating layers has hydrophobicity, and the hydrophobicity of the coating layers is enhanced with increasing ZnO composite content.
FIG. 4 shows the effect of different ZnO content on early anti-adhesion of Staphylococcus aureus and Escherichia coli. The composite ZnO coating material can inhibit early adhesion of staphylococcus aureus and escherichia coli, and the antibacterial capability of the composite ZnO coating material is gradually enhanced along with the increase of the composite content of the ZnO.
FIG. 5 shows the effect of different ZnO content groups on the antibacterial effect of Staphylococcus aureus and Escherichia coli. The composite ZnO coating material can inhibit the proliferation activity of staphylococcus aureus and escherichia coli, and the antibacterial capability of the composite ZnO coating material is gradually enhanced along with the increase of the composite content of ZnO.
FIG. 6 is a graph showing the effect of different ZnO content groups of inner wall coating materials on mouse fibroblast activity. The influence on the cell activity is not significantly different among the groups, and the ZnO composite has good cell compatibility.
Detailed Description
The invention is further illustrated by the following embodiments, which are to be understood as merely illustrative of the invention and not limiting thereof.
In the disclosure, PCL is short for polycaprolactone, PU is short for polyurethane, and PCL and ZnO are used as raw materials to obtain the PU waterway pipeline inner wall coating material with antibacterial activity.
In the embodiment of the invention, PCL and ZnO are used as raw materials, and CH is selected 2 Cl 2 The solution is used as a solvent of PCL, and the ZnO composite PCL coating material is obtained through physical stirring and drying at 60 ℃.
In the invention, the obtained inner wall coating material has good film forming property, simple synthesis process and lower cost.
In the invention, the obtained inner wall coating material has good antibacterial capability, can inhibit bacterial adhesion in early stage, can inhibit bacterial proliferation for a long time, and has good biocompatibility.
The present invention will be further illustrated by the following examples. It is also to be understood that the following examples are given solely for the purpose of illustration and are not to be construed as limitations upon the scope of the invention, since numerous insubstantial modifications and variations will now occur to those skilled in the art in light of the foregoing disclosure. The specific process parameters and the like described below are also merely examples of suitable ranges, i.e., one skilled in the art can make a suitable selection from the description herein and are not intended to be limited to the specific values described below. Unless defined or otherwise indicated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. In addition, any method and material similar or equivalent to those described may be used in the methods of the present invention.
Example 1 the method for preparing the coating composition for the inner wall of the waterway pipeline of the dental treatment table provided by the invention
A preparation method of an inner wall coating composition of a waterway pipeline of an stomatology treatment table comprises the following steps:
step one, 0.5g PCL is weighed and is processedDissolved in 10mLCH 2 Cl 2 Obtaining PCL solution;
step two, respectively weighing 0.1g, 0.3g and 0.5g of ZnO powder, adding the ZnO powder into the PCL solution prepared in the step one, and uniformly stirring at room temperature;
and thirdly, respectively soaking the PU in the mixed solution obtained in the second step, slowly pulling out, and then placing in a 60 ℃ oven for 12 hours, wherein the samples are respectively named as PCL, zn1, zn3 and Zn5.
As shown in fig. 1, the PU inner wall coating material prepared in example 1 can successfully prepare a uniform and stable surface coating material in microscopic image under a scanning electron microscope;
as shown in fig. 2, the XRD pattern of the inner wall coating material prepared in example 1 confirmed that ZnO was successfully loaded into the PU surface coating material;
as shown in fig. 3, the contact angle test results of the inner wall coating material prepared in example 1 show that each group of coating has hydrophobicity, and the hydrophobicity of the coating is enhanced with the increase of the ZnO composite content.
EXAMPLE 2 evaluation of antibacterial Properties of inner wall coating Material according to the invention
The antibacterial properties of the inner wall coating material were evaluated using the standard strains gram positive staphylococcus aureus (s.aureus, ATCC 25923) and gram negative escherichia coli (e.coli, ATCC 25922). Diluting bacteria in logarithmic growth phase to 10 with physiological saline 7 CFU/mL. The sample is sterilized by 75% alcohol and placed in 24-well plate, 1000. Mu.L of bacterial liquid is added dropwise into each well, and the mixture is placed in a shaking table at 37 ℃ for culture. After 2 hours of incubation, bacterial fluids were collected and diluted 10, 100 and 1000 fold in fold ratio. 100 mu L of diluted bacterial liquid is evenly coated on the surface of an agar plate, and the agar plate is placed into an incubator for culturing for 18 hours after being inverted. The number of colonies on the upper surface of the agar plates (GB/T4789.2) was counted, and the antibacterial ratio of the samples was calculated: antibacterial ratio (%) = (a-B)/a×100%, where a is the average number of colonies on the PU sample surface, and B is the average number of colonies on the test group sample surface.
As shown in FIG. 4, the inner wall coating materials of different ZnO content groups have the effect on early anti-adhesion of staphylococcus aureus and escherichia coli, the coating materials of the composite ZnO can inhibit the early adhesion of staphylococcus aureus and escherichia coli, and the antibacterial capability of the composite ZnO coating materials is gradually enhanced along with the increase of the composite content of ZnO.
Example 3 evaluation of antibacterial Properties of inner wall coating Material according to the invention
The antibacterial properties of the inner wall coating material were evaluated using the standard strains gram positive staphylococcus aureus (s.aureus, ATCC 25923) and gram negative escherichia coli (e.coli, ATCC 25922). Diluting bacteria in logarithmic growth phase to 10 with physiological saline 7 CFU/mL. The sample is sterilized by 75% alcohol and placed in a 24-well plate, 1000 mu L of bacterial liquid is dripped into each well, and the mixture is placed in a 37 ℃ incubator for culture. After 24 hours of incubation, bacterial fluids were collected and diluted 10, 100 and 1000 fold. 100 mu L of diluted bacterial liquid is evenly coated on the surface of an agar plate, and the agar plate is placed into an incubator for culturing for 18 hours after being inverted. The number of colonies on the upper surface of the agar plates (GB/T4789.2) was counted, and the antibacterial ratio of the samples was calculated: antibacterial ratio (%) = (a-B)/a×100%, where a is the average number of colonies on the PU sample surface, and B is the average number of colonies on the test group sample surface.
As shown in FIG. 5, the inner wall coating materials with different ZnO contents have the effect on the antibacterial effect on staphylococcus aureus and escherichia coli, the composite ZnO coating materials can inhibit the proliferation activity of staphylococcus aureus and escherichia coli, and the antibacterial capability of the composite ZnO coating materials is gradually enhanced along with the increase of the composite ZnO content.
EXAMPLE 4 evaluation of the cell compatibility of the inner wall coating Material provided by the invention
Mouse fibroblasts (L929) were used to evaluate the cytocompatibility of the inner wall coating material. Firstly preparing leaching liquor of the PU inner wall coating, respectively soaking each group of samples in 3mL of basic culture medium, taking supernatant after 12h and 24h, and filtering by a bacterial filter membrane to obtain leaching liquor. At 1X 10 3 Density of individual/well L929 was inoculated into 96 well cell culture plates and placed in a 37℃incubator at 5% CO 2 Is cultured in a moist environment for 24 hours. Each set of leach liquor concentrations was set up in 6 replicates. After 24h of cell attachment, the medium was changed to medium containing inner wall coating extracts of different compositions, 100. Mu.L per well, and after 24h, cell viability was detected using the CellCounting kit-8 (CCK 8, japan) kit. Using enzyme labelsThe absorbance (OD) was measured. The larger the OD value, the more representative of the cell number, the better the activity.
As shown in FIG. 6, the inner wall coating materials of groups with different ZnO contents have no obvious difference on the influence on the activity of the mouse fibroblast, and the ZnO composite has good cell compatibility.
EXAMPLE 5 evaluation of cell compatibility of inner wall coating Material provided by the present invention
The cell compatibility of the coating material was further assessed by cell viability/death staining, the specific procedure being as follows: (1) Culturing the cells with the leaching solution for 12h and 24h, sucking out the culture medium by using a liquid transfer device, and washing for 1 time by using PBS; (2) 100. Mu.L of cell live/dead dye was added per well;
(3) Incubating for 15min at 37 ℃;
(4) After 15min, the dye was aspirated with a pipettor, washed twice with PBS, and then stored protected from light;
(5) The sample was observed under a fluorescence microscope and photographed.
Claims (10)
1. The inner wall coating composition of the waterway pipeline of the dental treatment table is characterized by comprising PCL and ZnO, wherein the weight ratio of the ZnO to the PCL is 20-100%.
2. An inner wall coating composition of a waterway pipeline of an oral treatment table according to claim 1, wherein a weight ratio of ZnO to PCL is 30-90%.
3. An inner wall coating composition of a waterway pipeline of an oral treatment table according to claim 2, wherein a weight ratio of ZnO to PCL is 40-80%.
4. An inner wall coating composition for waterway tubing of an oral treatment table according to claim 3, wherein the weight ratio of ZnO to PCL is 50-70%.
5. An inner wall coating composition for a waterway conduit of an oral treatment table of claim 4, wherein a weight ratio of ZnO to PCL is 60%.
6. A method for preparing the coating composition for the inner wall of the waterway pipeline of the dental treatment table according to any one of claims 1 to 5, comprising the steps of:
step one, utilize CH 2 Cl 2 Dissolving PCL to obtain PCL solution;
step two, adding ZnO powder into the PCL solution, and uniformly stirring to obtain a mixed solution;
and thirdly, soaking the PU in the mixed solution, slowly pulling out, and drying to obtain the inner wall coating composition.
7. The process according to claim 6, wherein in the first step, CH is used at room temperature 2 Cl 2 Dissolve PCL.
8. The method according to claim 6, wherein in the third step, the specific drying process is as follows: and the mixture is placed in an oven at 60 ℃ for 12 hours.
9. Use of the inner wall coating composition of waterway tubing of an oral treatment table of any of claims 1-5 for antimicrobial.
10. Use according to claim 9, wherein the inner wall coating composition is used for the antibacterial treatment of waterway tubing of an oral treatment table.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106178136A (en) * | 2016-07-29 | 2016-12-07 | 许静宜 | A kind of medical hydrophilic antimicrobial coatings and preparation method thereof |
| CN114656864A (en) * | 2022-04-05 | 2022-06-24 | 江苏师范大学 | Super-hydrophobic magnesium alloy coating and process thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106178136A (en) * | 2016-07-29 | 2016-12-07 | 许静宜 | A kind of medical hydrophilic antimicrobial coatings and preparation method thereof |
| CN114656864A (en) * | 2022-04-05 | 2022-06-24 | 江苏师范大学 | Super-hydrophobic magnesium alloy coating and process thereof |
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