CN117343635A - Inner wall coating of waterway pipeline of stomatology treatment table, and preparation method and application thereof - Google Patents
Inner wall coating of waterway pipeline of stomatology treatment table, and preparation method and application thereof Download PDFInfo
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- CN117343635A CN117343635A CN202311461988.7A CN202311461988A CN117343635A CN 117343635 A CN117343635 A CN 117343635A CN 202311461988 A CN202311461988 A CN 202311461988A CN 117343635 A CN117343635 A CN 117343635A
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- pei
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- 238000000576 coating method Methods 0.000 title claims abstract description 112
- 239000011248 coating agent Substances 0.000 title claims abstract description 107
- 238000002360 preparation method Methods 0.000 title abstract description 8
- 230000000844 anti-bacterial effect Effects 0.000 claims abstract description 30
- 239000000243 solution Substances 0.000 claims abstract description 28
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims abstract description 12
- 238000005660 chlorination reaction Methods 0.000 claims abstract description 11
- 239000011259 mixed solution Substances 0.000 claims abstract description 10
- 229920000867 polyelectrolyte Polymers 0.000 claims abstract description 8
- 229920000642 polymer Polymers 0.000 claims abstract description 6
- 239000007864 aqueous solution Substances 0.000 claims abstract description 4
- 238000003756 stirring Methods 0.000 claims abstract description 3
- 238000000034 method Methods 0.000 claims description 19
- 230000008569 process Effects 0.000 claims description 7
- 238000002791 soaking Methods 0.000 claims description 6
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims 1
- 229920002873 Polyethylenimine Polymers 0.000 abstract description 19
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 abstract description 16
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 abstract description 16
- 235000002949 phytic acid Nutrition 0.000 abstract description 16
- 229940068041 phytic acid Drugs 0.000 abstract description 16
- 239000000467 phytic acid Substances 0.000 abstract description 16
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 6
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 3
- 125000003277 amino group Chemical group 0.000 abstract description 2
- 125000004433 nitrogen atom Chemical group N* 0.000 abstract description 2
- 239000000463 material Substances 0.000 description 42
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- 230000000694 effects Effects 0.000 description 25
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- 241000894006 Bacteria Species 0.000 description 16
- 241000191967 Staphylococcus aureus Species 0.000 description 16
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 13
- 229910052801 chlorine Inorganic materials 0.000 description 13
- 230000035755 proliferation Effects 0.000 description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 10
- 239000000126 substance Substances 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
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- 238000012258 culturing Methods 0.000 description 4
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 4
- 239000002953 phosphate buffered saline Substances 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- FFUAGWLWBBFQJT-UHFFFAOYSA-N hexamethyldisilazane Chemical compound C[Si](C)(C)N[Si](C)(C)C FFUAGWLWBBFQJT-UHFFFAOYSA-N 0.000 description 3
- 238000003973 irrigation Methods 0.000 description 3
- 230000002262 irrigation Effects 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- FVAUCKIRQBBSSJ-UHFFFAOYSA-M sodium iodide Chemical compound [Na+].[I-] FVAUCKIRQBBSSJ-UHFFFAOYSA-M 0.000 description 3
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 description 3
- 235000019345 sodium thiosulphate Nutrition 0.000 description 3
- VZSRBBMJRBPUNF-UHFFFAOYSA-N 2-(2,3-dihydro-1H-inden-2-ylamino)-N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]pyrimidine-5-carboxamide Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C(=O)NCCC(N1CC2=C(CC1)NN=N2)=O VZSRBBMJRBPUNF-UHFFFAOYSA-N 0.000 description 2
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 2
- 238000000026 X-ray photoelectron spectrum Methods 0.000 description 2
- 230000003698 anagen phase Effects 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 229910002091 carbon monoxide Inorganic materials 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 125000001309 chloro group Chemical group Cl* 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- OSVXSBDYLRYLIG-UHFFFAOYSA-N dioxidochlorine(.) Chemical compound O=Cl=O OSVXSBDYLRYLIG-UHFFFAOYSA-N 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 229910052740 iodine Inorganic materials 0.000 description 2
- 239000011630 iodine Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 210000000214 mouth Anatomy 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 229920002635 polyurethane Polymers 0.000 description 2
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- 206010001889 Alveolitis Diseases 0.000 description 1
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- 239000004155 Chlorine dioxide Substances 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 1
- 208000037581 Persistent Infection Diseases 0.000 description 1
- PLXBWHJQWKZRKG-UHFFFAOYSA-N Resazurin Chemical compound C1=CC(=O)C=C2OC3=CC(O)=CC=C3[N+]([O-])=C21 PLXBWHJQWKZRKG-UHFFFAOYSA-N 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 208000014151 Stomatognathic disease Diseases 0.000 description 1
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- 239000007844 bleaching agent Substances 0.000 description 1
- 229960003333 chlorhexidine gluconate Drugs 0.000 description 1
- YZIYKJHYYHPJIB-UUPCJSQJSA-N chlorhexidine gluconate Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O.C1=CC(Cl)=CC=C1NC(=N)NC(=N)NCCCCCCNC(=N)NC(=N)NC1=CC=C(Cl)C=C1 YZIYKJHYYHPJIB-UUPCJSQJSA-N 0.000 description 1
- 235000019398 chlorine dioxide Nutrition 0.000 description 1
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- 239000000645 desinfectant Substances 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
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- 238000001035 drying Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
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- 201000001155 extrinsic allergic alveolitis Diseases 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
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- 230000006872 improvement Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
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- 230000007774 longterm Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 238000009428 plumbing Methods 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 206010039083 rhinitis Diseases 0.000 description 1
- 235000009518 sodium iodide Nutrition 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
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- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- DHCDFWKWKRSZHF-UHFFFAOYSA-N sulfurothioic S-acid Chemical compound OS(O)(=O)=S DHCDFWKWKRSZHF-UHFFFAOYSA-N 0.000 description 1
- 239000008400 supply water Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/23—Solid substances, e.g. granules, powders, blocks, tablets
- A61L2/232—Solid substances, e.g. granules, powders, blocks, tablets layered or coated
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
- C09D179/00—Coating compositions based on macromolecular compounds obtained by reactions forming in the main chain of the macromolecule a linkage containing nitrogen, with or without oxygen, or carbon only, not provided for in groups C09D161/00 - C09D177/00
- C09D179/02—Polyamines
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
- C09D5/00—Coating compositions, e.g. paints, varnishes or lacquers, characterised by their physical nature or the effects produced; Filling pastes
- C09D5/14—Paints containing biocides, e.g. fungicides, insecticides or pesticides
Abstract
The invention relates to an inner wall coating of a waterway pipeline of an stomatology treatment table, a preparation method and application thereof, wherein the inner wall coating is an n-halamine polymer, and comprises phytic acid, polyethyleneimine and n-halamine of which the amino group is chlorinated. The preparation method comprises the following steps: adding PA and PEI into the aqueous solution, and uniformly stirring to obtain a mixed solution; immersing PU in the mixed solution, slowly pulling out, naturally airing to obtain a polyelectrolyte complex coating; and immersing the polyelectrolyte complex coating in NaClO solution for chlorination to obtain the inner wall coating. Compared with the surfaces of other antibacterial coatings, the inner wall coating provided by the invention can wash nitrogen atom sites in the repeatedly chlorinated PEI through daily disinfection measures NaClO solution, can meet the durable and renewable antibacterial requirements of waterway pipelines of the dental treatment table, and has important application value in thoroughly solving the waterway pollution problem of the dental treatment table.
Description
Technical Field
The invention belongs to the field of biomedical materials, and in particular relates to a coating for the inner wall of a waterway pipeline of an oral treatment table, a preparation method and application thereof, which can be applied to long-acting and circulating renewable antibacterial property of the waterway pipeline of the oral treatment table.
Background
The comprehensive oral treatment table is a medical device for dental treatment examination, diagnosis and treatment of various dental diseases. The dental treatment station waterway is a water input and discharge plumbing system for the oral integrated treatment station and may supply water to connected irrigation and refrigeration instruments, cup-shaped injection ports and bowl-shaped irrigation outlets for irrigation of the patient's oral and dental surfaces during operation. However, the dental handpiece and other connecting parts are difficult to disassemble and clean, and the oral cavity comprehensive treatment table has narrow and slender waterways, so that biofilm on the waterway surfaces is easy to form and continuously grow. Continuously contaminated treatment water increases the risk of cross-contamination of the oral care patient and medical personnel.
Biofilm is an important source of water pollution that causes waterway pollution in the dental treatment tables. It has been reported that bacteria in the water supply pipeline can reach 2×10 within five days after the new oral treatment chair is connected with the water supply pipeline 5 CFU/mL level. Most microorganisms in the water outlet of the treatment table waterway are low-pathogenicity gram-negative aerobic heterotrophic bacteria. The appearance type and range of the environmental bacteria are irregular, and are generally closely related to the layout setting, diagnosis and treatment flow and the like of the stomatology. Fungi, tissue-dissolving amoeba and saccharomycetes are also detected in the waterway of the treatment table. If the waterway of the dental treatment table is not effectively sterilized, the biofilm fragments therein may be transferred into the oral cavity of the patient, resulting in the occurrence of acute and chronic infections. Part of the biofilm can also be converted into part of aerosol, which is easily inhaled by dental office staff and patients, and induces the occurrence of asthma, rhinitis, allergic alveolitis and organic dust poisoning syndrome. Therefore, the waterway of the oral treatment table should be properly cleaned and disinfected to minimize biofilm and microorganism contamination and meet water use standards.
Common methods for disinfecting waterways of treatment tables can be divided into two types, namely non-chemical methods and chemical methods. Bacterial films attached to the surface of the pipeline can be effectively removed through ultraviolet irradiation, ozone sterilization, mechanical erasure and the like, however, the waterway pipeline of the treatment table is narrow and complex and difficult to detach, and the whole waterway is difficult to sterilize comprehensively and conveniently by an illegal chemical method. In connection with chemical sterilization, several sterilization products have been proposed which can be used in continuous chemical treatment processes, such as chlorine dioxide, hydrogen peroxide, chlorhexidine gluconate and iodine agents. The main mechanism of the strong oxidant is to destroy the structural function of bacteria and reduce the activity of the bacteria, oxidize microbial biomass films attached to the surface of a pipeline, and inhibit the function of proteins to kill microorganisms such as bacteria. The chemical method can realize excellent disinfection effect in a short time, is convenient and quick, however, the chemical disinfection method still faces the challenge of long-term bacteriostasis requirement, and the health risk caused by the fact that the concentration of partial chemical disinfectants such as iodine agents is increased is also a factor needing attention.
Therefore, how to endow the dental treatment table with the long-acting circulating reproducible antibacterial function is still the direction of the current improvement development of waterway pipelines.
Disclosure of Invention
Aiming at the problems, the invention aims to provide a long-acting circulated and regenerated antibacterial inner wall coating for a waterway pipeline of an stomatology treatment table, a preparation method and application thereof, which can effectively inhibit the growth of bacteria, prevent the formation of a biological film and realize the circulated and regenerated antibacterial effect of the coating through a simple chlorination process.
In a first aspect, the invention provides an inner wall coating of a waterway pipeline of an oral treatment table, wherein the inner wall coating is an n-halamine polymer and comprises Phytic Acid (PA), polyethyleneimine (PEI) and n-halamine of which the amino group is chlorinated, and the weight ratio of the PEI to the PA is 20-100%.
The weight ratio of PEI to PA is 20-100 percent: 20-100 parts by weight of PEI and 100 parts by weight of PA. The following is the same.
Preferably, the 20-100% range includes any particular point value attributed thereto, such as 20%, 21%, 25%, 27%, 30%, 33%, 35%, 38%, 40%, 42%, 45%, 47%, 50%, 52%, 55%, 57%, 58%, 60%, 62%, 65%, 68%, 70%, 72%, 73%, 75%, 77%, 80%, 82%, 85%, 86%, 88%, 90%, 92%, 95%, 98%, 99%, 100%, and ranges of any two of these particular point values, such as 25-95%, 30-80%, 30-70%, or 40-60%.
In a second aspect, the invention provides a preparation method of the inner wall coating of the waterway pipeline of the dental treatment table, comprising the following steps:
adding PA and PEI into the aqueous solution, and uniformly stirring to obtain a mixed solution;
step two, soaking PU (polyurethane) in the mixed solution, slowly pulling out, naturally airing to obtain a polyelectrolyte complex coating;
and thirdly, immersing the polyelectrolyte complex coating into NaClO solution for chlorination to obtain the inner wall coating.
Preferably, in the second step, the PU is soaked in the mixed solution for 4 hours.
Preferably, in the third step, the specific chlorination process is as follows: immersed in a 0.2% by mass/volume solution of NaClO for 10 minutes.
In a third aspect, the invention also provides an application of the inner wall coating of the waterway pipeline of the dental treatment table in antibiosis, in particular to an application of the waterway pipeline of the dental treatment table in antibiosis.
Compared with the prior art, the invention has the following beneficial effects:
(1) According to the invention, the strong negative charge of PA and the high positive charge density of PEI are utilized in an aqueous solution, an environment-friendly polyelectrolyte complex coating is synthesized on the surface of PU, and then the PU co-deposited by PA and PEI is immersed in sodium hypochlorite (NaClO) bleaching agent solution for chlorination under the mild environment of normal temperature and normal pressure, so that an n-halamine polymer coating is obtained, and the n-halamine polymer coating can be used as an inner wall coating of a waterway pipeline of an stomatology treatment table.
(2) When the inner wall coating is used for culturing staphylococcus aureus and escherichia coli, the proliferation of bacteria can be inhibited, and the antibacterial effect is good.
(3) When the inner wall coating provided by the invention is used for culturing the mouse fibroblast, no obvious cytotoxicity exists, and the biocompatibility is good.
(4) The inner wall coating provided by the invention can realize chlorination in the flushing of a NaClO solution with lower concentration (the mass volume ratio concentration is 0.2%) in a short time, release active chlorine, and recover and promote the antibacterial effect of the coating on staphylococcus aureus and escherichia coli.
(5) The inner wall coating provided by the invention can inhibit the proliferation of bacteria in a waterway pipeline of an oral treatment table, prevent the formation of a biological film, simultaneously can disinfect and flush through a simple and rapid NaClO solution, repeatedly chlorinate nitrogen atom sites in PEI, recover and promote the antibacterial effect of the coating in the chlorination process, realize a long-acting recyclable antibacterial function, and further have important application value in thoroughly solving the waterway pollution problem of the oral treatment table.
Drawings
FIG. 1 shows microscopic images of PU coating, PP coating and chlorinated PP coating (PP-Cl) material prepared in example 1 under a scanning electron microscope, which can smoothly prepare uniform and stable surface coating material.
FIG. 2 is an XPS spectrum of the inner wall coating material prepared in example 1, showing a distinct peak position attributed to chlorine atoms on the PP-Cl surface, confirming successful chlorination of the PP-Cl coating.
FIG. 3 is a PP-Cl inner wall coating material Cl 2p prepared in example 1 1/2 XPS high resolution spectrogram, XPS detects and shows that the PP-Cl surface contains N-Cl bond, which indicates that active chlorine is loaded successfully.
FIG. 4 is an optical photograph of the active chlorine of the PP-Cl inner wall coating material prepared in example 1, showing that the surface of the PP-Cl can release active chlorine.
FIG. 5 shows the effect of PU, PP and PP-Cl inner wall coating materials on Staphylococcus aureus and Escherichia coli proliferation. The cultivation time is 6 hours, and the density of the planted bacteria is 10 6 At CFU/mL, the PP and PP-Cl coating materials can inhibit the proliferation activity of staphylococcus aureus and escherichia coli.
FIG. 6 shows the effect of PU, PP and PP-Cl inner wall coating materials on Staphylococcus aureus and Escherichia coli proliferation. The cultivation time is 6 hours, and the density of the planted bacteria is 10 7 When CFU/mL, the PP and PP-Cl coating materials can inhibit goldThe proliferation activity of staphylococcus aureus and escherichia coli, and the antibacterial performance of the chlorinated PP-Cl coating is obviously better than that of a PP coating material.
FIG. 7 shows the effect of PU, PP and PP-Cl inner wall coating materials on the bacterial morphology of Staphylococcus aureus and Escherichia coli. Compared with the PU coating, the bacterial quantity on the surfaces of the PP and PP-Cl coating materials is obviously reduced, the bacterial quantity on the surfaces of the PP-Cl coating materials is minimum, and the bacterial structure is obviously destroyed, so that the PP and PP-Cl coatings have good antibacterial effects, and the antibacterial effects of the PP-Cl coatings are stronger.
FIG. 8 is the effect of PU, PP and PP-Cl inner wall coating materials on L929 mouse fibroblast activity. After 24 hours of culture, the proliferation activity of L929 cells can be obviously improved by the PU, PP and PP-Cl inner wall coating materials, and after 48 hours of culture, the influence on the cell activity is not obviously different among groups.
Detailed Description
The invention is further illustrated by the following embodiments, which are to be understood as merely illustrative of the invention and not limiting thereof.
In the disclosure, PU is short for polyurethane, PA is short for phytic acid, PEI is short for polyethyleneimine, and PA and PEI are used as raw materials to obtain a PA@PEI polyelectrolyte complex loaded PU waterway pipeline inner wall coating material with antibacterial activity, which is called PP coating.
In the embodiment of the invention, naClO solution is selected as a chloridizing solvent of the PP coating, and the n-halamine polymer coating is obtained through soaking and natural drying, which is called PP-Cl coating.
In the invention, the obtained inner wall coating material has good film forming property, simple synthesis process and lower cost.
In the invention, the obtained inner wall coating material has good antibacterial capability, can inhibit bacterial proliferation, and has good biocompatibility.
In the invention, the obtained inner wall coating material can recover and improve the antibacterial effect of the coating simply and efficiently by being soaked in NaClO solution.
The present invention will be further illustrated by the following examples. It is also to be understood that the following examples are given solely for the purpose of illustration and are not to be construed as limitations upon the scope of the invention, since numerous insubstantial modifications and variations will now occur to those skilled in the art in light of the foregoing disclosure. The specific process parameters and the like described below are also merely examples of suitable ranges, i.e., one skilled in the art can make a suitable selection from the description herein and are not intended to be limited to the specific values described below. Unless defined or otherwise indicated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. In addition, any method and material similar or equivalent to those described may be used in the methods of the present invention.
Example 1 the method for preparing the coating on the inner wall of the waterway pipeline of the dental treatment table provided by the invention
A preparation method of an inner wall coating of a waterway pipeline of an stomatology treatment table comprises the following steps:
firstly, 1mL of 50% PA solution is measured and dissolved in 49mL of pure water to obtain PA solution;
step two, weighing 200mg of PEI, and dissolving the PEI in 40mL of pure water to obtain PEI solution;
step three, uniformly mixing the PA and PEI solutions obtained in the step one and the step two, soaking PU in the mixed solution, keeping for 4 hours, slowly pulling out, naturally airing, and obtaining a sample named PP;
step three, immersing PP in a NaClO solution with the concentration of 0.2%, keeping for 10 minutes, slowly pulling out, naturally airing, and obtaining a sample named PP-Cl.
As shown in FIG. 1, the PU coating, the PP coating and the PP-Cl coating prepared in the embodiment 1 can successfully prepare uniform and stable surface coating materials under microscopic images of a scanning electron microscope.
As shown in FIG. 2, the XPS spectrum of the inner wall coating material prepared in example 1 shows obvious peak positions attributed to chlorine atoms on the surface of PP-Cl, and the successful chlorination of the PP-Cl coating is confirmed.
As shown in FIG. 3, the PP-Cl inner wall coating material Cl 2p prepared in example 1 1/2 XPS high resolution spectrogram shows that the surface of PP-Cl contains N-Cl bond, and active chlorine is loaded successfully.
EXAMPLE 2 evaluation of the active chlorine content Performance of the inner wall coating provided by the invention
The active chlorine content of the samples was quantified by iodometric/thiosulfate titration. The PP-Cl chlorinated coating material was immersed in 30mL of 1% aqueous sodium iodide solution for 10 minutes to form I 2 . Then, 1mL of a 1% starch solution was added to the above solution until the color of the solution changed from transparent to blue. Then, the solution was titrated with a sodium thiosulfate solution (the solution color changed from blue to colorless). Cl in the sample + The content is calculated according to the following formula;
wherein Cl + (μM/cm 2 ) Is the concentration of active chlorine in the sample, N and V are the concentration and volume of titrant sodium thiosulfate, respectively, S is the area of the sample 33.91cm 2 。
As shown in FIG. 4, an optical photograph for detecting active chlorine of the PP-Cl inner wall coating material prepared in example 1 shows that the surface of the PP-Cl can release active chlorine.
The concentration of active chlorine corresponding to the titration volume of sodium thiosulfate in Table 1 was measured and indicated that active chlorine was released from the PP-Cl coating surface.
TABLE 1
| Drop volume | 13.6 | 13.3 | 12.9 |
| Concentration of active chlorine | 0.200 | 0.196 | 0.190 |
Example 3 evaluation of antibacterial Properties of inner wall coating provided by the invention
The antibacterial properties of the inner wall coating material were evaluated using the standard strains gram positive staphylococcus aureus (s.aureus, ATCC 25923) and gram negative escherichia coli (e.coli, ATCC 25922). Diluting bacteria in logarithmic growth phase to 10 with physiological saline 6 CFU/mL. The sample is sterilized by 75% alcohol and placed in a 24-well plate, 500 mu L of bacterial liquid is dripped into each well, and the mixture is placed in a 37 ℃ incubator for culture. After 6 hours of incubation, bacterial fluids were collected and diluted 10, 100 and 1000 fold. 100 mu L of diluted bacterial liquid is evenly coated on the surface of an agar plate, and the agar plate is placed into an incubator for culturing for 18 hours after being inverted. The number of colonies on the upper surface of the agar plates (GB/T4789.2) was counted, and the antibacterial ratio of the samples was calculated: antibacterial ratio (%) = (a-B)/a×100%, where a is the average number of colonies on the PU sample surface, and B is the average number of colonies on the test group sample surface.
As shown in FIG. 5, the effects of PU, PP and PP-Cl inner wall coating materials on Staphylococcus aureus and Escherichia coli proliferation. The cultivation time is 6 hours, and the density of the planted bacteria is 10 6 At CFU/mL, the PP and PP-Cl coating materials can inhibit the proliferation activity of staphylococcus aureus and escherichia coli.
Example 4 evaluation of antibacterial Properties of inner wall coating provided by the invention
The antibacterial properties of the inner wall coating material were evaluated using the standard strains gram positive staphylococcus aureus (s.aureus, ATCC 25923) and gram negative escherichia coli (e.coli, ATCC 25922). Diluting bacteria in logarithmic growth phase to 10 with physiological saline 7 CFU/mL. Sterilizing the sample with 75% alcohol, placing in 24-well plate, and dripping 5 times into each wellmu.L of the bacterial liquid was cultured in an incubator at 37 ℃. After 6 hours of incubation, bacterial fluids were collected and diluted 10, 100 and 1000 fold. 100 mu L of diluted bacterial liquid is evenly coated on the surface of an agar plate, and the agar plate is placed into an incubator for culturing for 18 hours after being inverted. The number of colonies on the upper surface of the agar plates (GB/T4789.2) was counted, and the antibacterial ratio of the samples was calculated: antibacterial ratio (%) = (a-B)/a×100%, where a is the average number of colonies on the PU sample surface, and B is the average number of colonies on the test group sample surface.
As shown in FIG. 6, the effects of PU, PP and PP-Cl inner wall coating materials on Staphylococcus aureus and Escherichia coli proliferation. The cultivation time is 6 hours, and the density of the planted bacteria is 10 7 When CFU/mL is carried out, the PP and the PP-Cl coating materials can inhibit the proliferation activity of staphylococcus aureus and escherichia coli, and the antibacterial performance of the chlorinated PP-Cl coating is obviously better than that of the PP coating material.
EXAMPLE 5 evaluation of antibacterial Properties of inner wall coating provided by the invention
The antibacterial properties of the inner wall coating material were evaluated using the standard strains gram positive staphylococcus aureus (s.aureus, ATCC 25923) and gram negative escherichia coli (e.coli, ATCC 25922). The samples were sterilized with 75% ethanol for 2 hours and then dried and placed in 24 well plates. 100. Mu.L of E.coli and Staphylococcus aureus bacterial suspension (10) 7 CFU/mL) were inoculated onto the surfaces of each group of samples, and incubated in a constant temperature incubator at 37℃for 6 hours. The samples were then washed twice with Phosphate Buffered Saline (PBS) and transferred to a new 24-well plate, and 1mL of 2.5% glutaraldehyde solution was added to each well to fix the bacteria for 4 hours. The samples were then dehydrated with gradient ethanol solutions of 30%, 50%, 75%, 90%, 95% and 100% volume fractions, and then placed in a mixed solution of ethanol and Hexamethyldisilazane (HMDS) (ethanol: hmds=2:1, 1:1,1:2 v/v) and 100% HMDS solution for dehydration, and the dehydrated and dried samples were subjected to surface gold spraying (platinum), and the surface bacterial morphology of each group of samples was observed using a scanning electron microscope.
As shown in FIG. 7, the effects of PU, PP and PP-Cl inner wall coating materials on the bacterial morphology of Staphylococcus aureus and Escherichia coli. Compared with the PU coating, the bacterial quantity on the surfaces of the PP and PP-Cl coating materials is obviously reduced, the bacterial quantity on the surfaces of the PP-Cl coating materials is minimum, and the bacterial structure is obviously destroyed, so that the PP and PP-Cl coatings have good antibacterial effects, and the antibacterial effects of the PP-Cl coatings are stronger.
EXAMPLE 6 evaluation of cell compatibility of inner wall coating provided by the present invention
The cell compatibility of the inner wall coating material was evaluated using L929 mouse fibroblasts. Firstly preparing leaching liquor of the PU inner wall coating, respectively soaking each group of samples in 3mL of basic culture medium, taking supernatant after 48 hours, and filtering by a bacterial filter membrane to obtain leaching liquor. At 1X 10 4 Density of individual/well L929 was inoculated into 96 well cell culture plates, placed in a 37℃incubator at 5% CO 2 Is cultured in a moist environment for 24 hours. Each set of leach liquor concentrations was set up in 6 replicates. After 24 hours of cell attachment, the medium was changed to medium containing different components of inner wall coating extract, 100 μl per well, and after 24 hours the cell activity was measured using AlamarBlue kit. Fluorescence intensity was measured using an enzyme-linked immunosorbent assay. The larger the fluorescence intensity value, the more the number of cells, the better the activity.
As shown in FIG. 8, the effect of PU, PP and PP-Cl inner wall coating materials on L929 mouse fibroblast activity. After 24 hours of culture, the proliferation activity of L929 cells can be obviously improved by the PU, PP and PP-Cl inner wall coating materials, and after 48 hours of culture, the influence on the cell activity is not obviously different among groups.
EXAMPLE 7 evaluation of cell compatibility of inner wall coating provided by the present invention
The cell compatibility of the coating material was further assessed using L929 mouse fibroblast cell live/dead staining. Firstly preparing leaching liquor of the PU inner wall coating, respectively soaking each group of samples in 3mL of basic culture medium, taking supernatant after 48 hours, and filtering by a bacterial filter membrane to obtain leaching liquor. At 1X 10 4 Density of individual/well L929 was inoculated into 96 well cell culture plates, placed in a 37℃incubator at 5% CO 2 Is cultured in a moist environment for 24 hours. After the cells are attached, the culture medium is replaced by the leaching solution, and the cells are cultured for 24 hours and 48 hours with the leaching solution, and the cells are sucked out by a liquid transfer deviceCulture medium, PBS wash 1 time; subsequently, 100. Mu.L of cell live/dead dye was added to each well and incubated at 37℃for 15 minutes in the absence of light. After 15 minutes, the dye was aspirated with a pipettor, washed twice with PBS, and then stored protected from light; the sample was observed under a fluorescence microscope and photographed.
As shown in FIG. 8, the effect of PU, PP and PP-Cl inner wall coating materials on L929 mouse fibroblast activity. After 24 hours and 48 hours of culture, a large amount of green fluorescence of marked living cells appears on the surface cells of each group of samples, the red fluorescence of marked dead cells is less, and the influence of each group on the cell activity is not obviously different.
Claims (10)
1. The inner wall coating of the waterway pipeline of the dental treatment table is characterized by being an n-halamine polymer and comprising PA, PEI and n-halamine with chlorinated amino, wherein the weight ratio of PEI to PA is 20-100%.
2. An inner wall coating of a waterway pipeline of an oral treatment table according to claim 1, wherein the weight ratio of PEI to PA is 25-95%.
3. An inner wall coating of a waterway pipeline of an oral treatment table according to claim 2, wherein the weight ratio of the PEI to the PA is 30-80%.
4. A coating for the inner wall of a waterway conduit of an oral treatment table according to claim 3, wherein the weight ratio of PEI to PA is 30-70%.
5. An inner wall coating for a waterway conduit of an oral treatment table according to claim 4, wherein the weight ratio of PEI to PA is 40-60%.
6. An interior wall coating for a waterway conduit of an oral treatment table of claim 5, wherein the weight ratio of PEI to PA is 50%.
7. The method for preparing the coating on the inner wall of the waterway pipeline of the dental treatment table according to any one of claims 1 to 6, which is characterized by comprising the following steps:
adding PA and PEI into the aqueous solution, and uniformly stirring to obtain a mixed solution;
step two, soaking PU in the mixed solution, slowly pulling out, naturally airing to obtain a polyelectrolyte complex coating;
and thirdly, immersing the polyelectrolyte complex coating into NaClO solution for chlorination to obtain the inner wall coating.
8. The method according to claim 7, wherein in the second step, PU is immersed in the mixed solution for 4 hours.
9. The method according to claim 7, wherein in the third step, the specific chlorination process is as follows: immersed in a 0.2% by mass/volume solution of NaClO for 10 minutes.
10. Use of the inner wall coating of the waterway pipeline of the dental treatment table according to any one of claims 1 to 6 for antibacterial.
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| CN202311461988.7A CN117343635A (en) | 2023-11-06 | 2023-11-06 | Inner wall coating of waterway pipeline of stomatology treatment table, and preparation method and application thereof |
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