CN115946387B - High-activity stevioside extraction method and application thereof - Google Patents

High-activity stevioside extraction method and application thereof Download PDF

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Publication number
CN115946387B
CN115946387B CN202211053297.9A CN202211053297A CN115946387B CN 115946387 B CN115946387 B CN 115946387B CN 202211053297 A CN202211053297 A CN 202211053297A CN 115946387 B CN115946387 B CN 115946387B
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stevioside
extrusion
stevia rebaudiana
squeezing
solution
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CN115946387A (en
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赵新峰
姚五洲
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Shandong Shengxiangyuan Biotechnology Co ltd
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Shandong Shengxiangyuan Biotechnology Co ltd
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Abstract

The application relates to the technical field of stevioside, and particularly discloses a high-activity stevioside extraction method and application thereof; the extraction method comprises 1) drying stevia leaf, pulverizing, and sieving; 2) Soaking sieved stevia rebaudiana leaves in the extracting solution, taking out the stevia rebaudiana leaves after soaking, squeezing twice, and mixing the squeezing liquid obtained by squeezing twice with the soaking solution to obtain the extracting solution; 3) Centrifuging the obtained extract to remove impurities, and decolorizing and deodorizing the centrifugate after removing the impurities; 4) Sequentially washing and analyzing the extracting solution, and concentrating the analyzing solution in vacuum; 5) Spraying the concentrated solution into spray drying tower to obtain high-activity stevioside; the extraction method can fully extract the stevioside of the stevia rebaudiana leaves, so that the extraction rate of the stevioside is greatly improved, and the extraction process of the stevioside is simple and does not have the condition of high temperature and high pressure, thereby effectively ensuring the activity of the stevioside obtained by extraction.

Description

High-activity stevioside extraction method and application thereof
Technical Field
The application relates to the technical field of stevioside, and particularly discloses a high-activity stevioside extraction method and application thereof.
Background
Stevioside is a natural sweetener extracted from stevia rebaudiana plant with high sweetness and low calorie and no side effect on human body, so that the stevioside is widely applied to various products such as food and beverage, oral liquid, chewing gum, lozenge and the like to replace chemical synthesis sweetener products such as aspartame, saccharin sodium, sodium cyclamate and the like. Medical researchers believe that stevioside from stevia is safer to humans than artificial sweeteners.
The traditional stevioside extraction method adopts an extraction method that stevia rebaudiana leaves are soaked in a tank, the water consumption is high, and salt is added into the leaching solution to remove impurities.
The application patent with application number 201610402354.8 discloses a process for extracting stevioside, which comprises the following steps: (1) Placing stevia rebaudiana leaves in a leaching column, adding water, pressurizing and countercurrent extracting; (2) decolorizing and deodorizing the leaching solution; (3) Industrial preparative chromatography separation: a. placing the sugar solution obtained in the step (2) on an industrial chromatographic column for sample loading; b. eluting with 55-60% high-concentration ethanol, filtering with membrane, diluting the non-permeable sugar-containing eluent with 1-2 times of water, and refining by the method of step (4); c. eluting with 25-35% low concentration ethanol solution, collecting sugar-containing eluent, filtering with membrane, recovering ethanol from the eluent, loading on chromatographic column, and eluting according to the method of step (3); (4) Decolorizing, desalting, deodorizing and concentrating the liquid obtained in the step (3); and (5) filtering and drying. The purity of the product prepared by the extraction method disclosed by the application is more than 90%, the process is simplified, the use amount of solvents such as water and ethanol is reduced, and the production cost is low, but the stevia rebaudiana is only directly placed in a leaching column for extraction, so that the effective components in the stevia rebaudiana cannot be fully extracted, and the extraction rate of the stevia rebaudiana is low.
The existing high-activity stevioside extraction device cannot balance the need of fully squeezing stevia rebaudiana leaves and the fact that the stevia rebaudiana leaves cannot be excessively squeezed, so that the extraction efficiency of stevioside is low, and the fact that the squeezing ratio is too large can lead to too fast heating of materials and cause deterioration of effective components in juice due to too high temperature.
Therefore, the application provides a high-activity stevioside extraction method and application thereof, aiming at the defects of the traditional stevioside extraction method, the existing stevioside extraction process and device.
Disclosure of Invention
The application aims at solving the problems of the traditional stevioside extraction method and the existing stevioside extraction process, and provides a high-activity stevioside extraction method and application thereof.
The application is realized by the following technical scheme:
a device for extracting high-activity stevioside is a spiral squeezing device and comprises a frame and a material receiving box.
Further, a first screw press device and a second screw press device are arranged in parallel in front of and behind the upper end of the frame.
Further, the same side end parts of the first spiral pressing device and the second spiral pressing device are connected through a U-shaped conveying pipe.
Still further, the first screw press device includes a first press housing fixedly mounted in the frame.
Further, the lower end of the first press case is connected with a first pulp guiding hopper which is arranged in a left-low and right-high mode, and the lower end of the first pulp guiding hopper is connected with a first discharging pipe.
The application also provides an extraction method adopting the device for extracting the high-activity stevioside, which comprises the following steps:
1) Weighing a certain amount of stevia rebaudiana leaves, drying the stevia rebaudiana leaves, crushing and sieving the dried stevia rebaudiana leaves for later use;
2) Soaking sieved stevia leaves in the extracting solution, taking out the stevia leaves after soaking, sequentially performing primary pressing and secondary pressing in the spiral pressing equipment of any one of claims 1-5, and mixing the pressing solution obtained by the two pressing with the soaking solution to obtain the extracting solution;
3) Centrifuging the obtained extract to remove impurities, and decolorizing and deodorizing the centrifugate after removing the impurities;
4) Sequentially washing columns and analyzing the decolorized and deodorized extracting solution, and concentrating the analyzing solution in vacuum;
5) And finally, spraying powder on the concentrated solution in a spray drying tower, and converting sugar solution into powdery solid to obtain the high-activity stevioside.
Further, the water content of the stevia rebaudiana leaves after drying in the step 1) is controlled to be 18-32%, and the mesh number of the dried stevia rebaudiana leaves She Guoshai is 100-200.
Further, the temperature of the vacuum concentration of the resolving liquid in the step 4) is controlled between 56 ℃ and 64 ℃.
Furthermore, the application also provides an application of the high-activity stevioside prepared by the method in food.
The beneficial effects are that:
1) According to the high-activity stevioside extraction method adopted by the application, firstly, the stevioside of the stevioside is dried to remove water, then the stevioside is crushed and then soaked in the extracting solution, after soaking for a period of time, the squeezing solution is squeezed twice by using the screw squeezing equipment, the squeezing solution is mixed with the extracting solution, and the mixed extracting solution is subjected to a series of impurity removal and purification operations.
2) According to the preparation device disclosed by the application, when stevia rebaudiana leaves are squeezed, raw materials are conveyed to the squeezing die head through the feeding spiral leaves, as the circular table holes matched with the tower-type variable-diameter squeezing spiral leaves are formed in the squeezing die head, stevia She Jin is squeezed once under the squeezing action of the two spiral leaves, and the squeezing die head is connected with the sealing ring block through the guide post and the regulating spring in the primary squeezing process, so that the squeezing die head has a certain retraction space, when the squeezing ratio is overlarge, the regulating spring can be compressed and moved outwards, and the deterioration of active ingredients in juice due to overhigh temperature caused by too high material heating due to overlarge squeezing ratio is effectively prevented; in addition, the materials are extruded by the diameter-variable and distance-variable extrusion spiral blades in the second extrusion barrel in the whole process, and the effective components in the stevia rebaudiana leaves are fully extruded in advance under the condition of effectively controlling the extrusion temperature, so that the extraction rate of the stevia rebaudiana is greatly improved.
3) The preparation device with the size design disclosed by the application can balance the need of fully squeezing the stevia rebaudiana leaves and the inability of excessively squeezing the stevia rebaudiana leaves when squeezing the stevia rebaudiana leaves, improves the extraction rate of the stevioside, and simultaneously avoids the deterioration of active ingredients in juice caused by too high temperature due to too high material heating caused by too high squeezing ratio.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed for the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a flowchart of the high activity stevioside extraction process of the present application;
FIG. 2 is a schematic view of a first angular perspective structure of the manufacturing apparatus according to the present application;
FIG. 3 is a schematic view of a second perspective view of the apparatus according to the present application;
FIG. 4 is a schematic perspective view of a first screw press according to the present application;
FIG. 5 is a schematic view showing the internal planar structure of a first screw press according to the present application;
FIG. 6 is a perspective exploded view of a first extruder barrel, a sealing ring block, a pulp extrusion die head, etc. according to the present application;
FIG. 7 is a schematic view of the internal plan structure of the first extrusion barrel of the present application;
FIG. 8 is a schematic diagram showing the connection of the first extruder barrel, the second extruder barrel and the U-shaped conveying pipe;
FIG. 9 is a schematic perspective view of a U-shaped conveying pipe according to the present application;
FIG. 10 is a schematic view of the internal plan structure of a second extrusion barrel according to the present application;
FIG. 11 is a schematic perspective view of an arc-shaped brush plate, brush filaments and protrusions according to the present application;
FIG. 12 is a schematic illustration of the internal planar structure of the first extruder barrel of the present application.
Wherein:
100-frames, 101-supporting frames and 102-bearing plates; 200-receiving box;
300-first screw press device, 301-first press case, 302-first pulp guiding hopper, 303-first discharge pipe, 304-first extrusion cylinder, 305-feeding hopper, 306-first driving device, 3071-feeding screw blade, 3072-tower variable diameter extrusion screw blade, 308-sealing ring block, 3081-round hole, 309-pulp extrusion die head, 310-insertion pipe, 311-guide column and 312-adjusting spring;
400-second screw press device, 401-second press case, 402-second extrusion cylinder, 4021-whole extrusion cavity, 403-shaft rod insertion tube, 404-second driving device, 405-rotating rod, 406-variable diameter variable distance extrusion screw blade, 407-joint tube, 408-slag discharge tube;
500-U-shaped material conveying pipes, 601-arc-shaped brush plates, 602-brush wires, 603-convex blocks, 604-transmission screw rods and 605-cleaning motors.
Detailed Description
In order that those skilled in the art will better understand the present application, a technical solution in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in which it is apparent that the described embodiments are only some embodiments of the present application, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present application without making any inventive effort, shall fall within the scope of the present application.
It should be noted that, without conflict, the embodiments of the present application and features of the embodiments may be combined with each other. The present application will be described in detail with reference to fig. 1 to 11 in conjunction with examples.
Example 1
The embodiment 1 discloses a method for extracting high-activity stevioside, which can refer to fig. 1, and comprises the following steps:
s1, weighing a certain amount of stevia rebaudiana leaves, drying the stevia rebaudiana leaves, crushing and sieving the dried stevia rebaudiana leaves for later use; wherein the water content of the dried stevia rebaudiana leaves is controlled to be 20%, and the mesh number of the dried stevia rebaudiana leaves She Guoshai is 120 mesh;
s2, soaking the sieved stevia rebaudiana leaves in the extracting solution, fishing out the stevia rebaudiana leaves after soaking, putting the stevia rebaudiana leaves into spiral squeezing equipment, sequentially carrying out primary squeezing and secondary squeezing, and mixing the squeezing solution obtained by the two squeezing with the soaking solution to obtain the extracting solution;
s3, centrifugally separating the obtained extracting solution to remove impurities, and decoloring and deodorizing the centrifugal solution after removing the impurities;
and S4, sequentially washing and analyzing the decolorized and deodorized extracting solution, vacuum concentrating the analyzed solution, and controlling the vacuum concentration temperature of the analyzed solution to be 58 ℃.
And S5, finally, spraying powder on the concentrated solution in a spray drying tower, and converting sugar solution into powdery solid to obtain the high-activity stevioside.
Example 2
This example 2 discloses a method for extracting high-activity stevioside, which can refer to fig. 1, and comprises the following steps:
s1, weighing a certain amount of stevia rebaudiana leaves, drying the stevia rebaudiana leaves, crushing and sieving the dried stevia rebaudiana leaves for later use; wherein the water content of the dried stevia rebaudiana leaves is controlled to be 26%, and the mesh number of the dried stevia rebaudiana leaves She Guoshai is 160 mesh;
s2, soaking the sieved stevia rebaudiana leaves in the extracting solution, fishing out the stevia rebaudiana leaves after soaking, putting the stevia rebaudiana leaves into spiral squeezing equipment, sequentially carrying out primary squeezing and secondary squeezing, and mixing the squeezing solution obtained by the two squeezing with the soaking solution to obtain the extracting solution;
s3, centrifugally separating the obtained extracting solution to remove impurities, and decoloring and deodorizing the centrifugal solution after removing the impurities;
and S4, sequentially washing and analyzing the decolorized and deodorized extracting solution, vacuum concentrating the analyzed solution, and controlling the vacuum concentration temperature of the analyzed solution to be 62 ℃.
And S5, finally, spraying powder on the concentrated solution in a spray drying tower, and converting sugar solution into powdery solid to obtain the high-activity stevioside.
In summary, the high-activity stevioside extraction method adopted in the embodiment 1 and the embodiment 2 is that the stevioside of the stevioside is firstly dried to remove moisture, then the stevioside is crushed and then soaked in the extracting solution, the soaking is carried out for a period of time, then the squeezing solution is squeezed twice by using a screw squeezing device, the squeezing solution and the extracting solution are mixed, and the mixed extracting solution is subjected to a series of impurity removal and purification operations.
Example 3
This example 3 discloses a production apparatus which is the screw press apparatus in step 2, and is described in detail below with reference to fig. 2 to 11.
Referring to fig. 2 and 3, the main body of the apparatus includes a frame 100 and a receiving box 200, a first screw press 300 and a second screw press 400 are provided side by side in front of and behind the upper end of the frame 100, and the same side ends of the first screw press 300 and the second screw press 400 are connected by a U-shaped feed pipe 500.
Referring to fig. 4, 5, 6 and 7, the first screw press apparatus 300 includes a first press housing 301, the frame 100 includes supporting frames 101 at left and right ends and a supporting plate 102 fixed to the two supporting frames 101, a mounting hole for mounting the first press housing 301 is formed in the supporting plate 102, and the first press housing 301 is fixedly mounted on the supporting plate 102 through the mounting hole.
A first pulp hopper 302 is connected to the lower end of the first press housing 301, and a first discharge pipe 303 is connected to the lower end of the first pulp hopper 302, and the material receiving box 200 is disposed directly below the first discharge pipe 303 to receive the press liquid. A first extrusion cylinder 304 is fixedly installed in the first press housing 301, a large number of liquid outlet holes are formed in the lower half portion of the first extrusion cylinder 304, a feeding hopper 305 is connected to the upper surface of the left end of the first extrusion cylinder 304, and the feeding hopper 305 extends out of the upper surface of the first press housing 301.
A sealing bearing is arranged on the left end face of the first extrusion barrel 304, a first driving device 306 is arranged on the left end face of the first press case 301, and a feeding screw blade 3071 is connected to the end part of an output shaft of the first driving device 306 extending into the inner cavity of the first extrusion barrel 304 through the sealing bearing. When the device is specifically arranged, the first driving device 306 comprises a reduction gearbox and a driving motor, the driving motor is connected with an input shaft of the reduction gearbox, and an output shaft of the reduction gearbox is connected with the feeding spiral blade 3071. Then, a tower-type reducing extrusion screw 3072 is connected to the right end of the feeding screw 3071.
The right end of the first extrusion cylinder 304 is fixedly connected with a sealing ring block 308, a plurality of round holes 3081 are formed in the inner side surface of the sealing ring block 308 in an annular array, a pulp extrusion die head 309 is arranged at the right end of the inner cavity of the first extrusion cylinder 304, the outer wall of the pulp extrusion die head 309 is attached to the inner cavity of the first extrusion cylinder 304, a round table hole 3091 with wide left and narrow right is formed in the pulp extrusion die head 309, the right end of the pulp extrusion die head 309 is connected with a splicing pipe 310 extending out of the sealing ring block 308, the right end surface of the pulp extrusion die head 309 is connected with guide columns 311 inserted into each corresponding round hole 3081, each guide column 311 is sleeved with an adjusting spring 312, and two ends of the adjusting spring 312 are respectively connected with the inner walls of the pulp extrusion die head 309 and the round holes 3081.
The second screw press 400 is similar to the first screw press 300 in construction and includes a second press housing 401 fixedly mounted in the frame 100, the second press housing 401 being also fixedly mounted to the carrier plate 102 through a mounting opening. A second pulp hopper arranged at the left lower side and the right higher side is connected to the lower end of the second press case 401, and a second discharge pipe is connected to the lower end of the second pulp hopper and is also arranged right above the material receiving box 200.
Referring to fig. 10, a second extrusion cylinder 402 is fixedly installed in a first press housing 401, a large number of liquid outlet holes are formed in the lower half of the second extrusion cylinder 402, and a full-range extrusion chamber 4021 with a narrow left and a wide right is formed in the second extrusion cylinder 402.
Referring to fig. 8 and 9, one end of the U-shaped feed pipe 500 is connected to an end of the insertion tube 310, and the other end is connected to a right end of the second squeeze tube 402. The right end of the U-shaped conveying pipe 500 is connected with a shaft rod inserting pipe 403 which is positioned on the same central axis as the second extrusion barrel 402, and a second driving device 404 is arranged at the outer end of the shaft rod inserting pipe 403, and the structural design of the second driving device 404 is the same as that of the first driving device. The output shaft of the second driving device 404 is connected with a rotating rod 405, the rotating rod 405 passes through the shaft rod inserting tube 403 and extends into the whole-process extrusion cavity 4021, the end part of the rotating rod 405 is connected with a variable-diameter variable-pitch extrusion spiral blade 406, the diameter and the pitch of the variable-diameter variable-pitch extrusion spiral blade 406 are gradually reduced from right to left, the left end of the second extrusion barrel 402 extending out of the second extrusion machine case 401 is connected with a joint tube 407, and the joint tube 407 is connected with a slag discharging tube 408.
Finally, the preparation device is also provided with cleaning devices for dredging the liquid outlet holes in the first press case 301 and the second press case 401. Referring to fig. 5 and 11, the cleaning device comprises an arc-shaped brush plate 601 arranged below a first extrusion cylinder 304 or a second extrusion cylinder 402, a large number of brush wires 602 are arranged on the upper surface of the arc-shaped brush plate 601, two protruding blocks 603 are connected to the lower surface of the arc-shaped brush plate 601, guide sliding holes and threaded holes are respectively formed in the two protruding blocks 603, guide sliding rods matched with the guide sliding holes and the threaded holes and a transmission screw rod 604 are arranged in the first press case 301 and the second press case 401, the end part of the transmission screw rod 604 is connected with a cleaning motor 605, the transmission screw rod 604 is driven to rotate forwards and backwards through the cleaning motor 605, then the arc-shaped brush plate 601 moves left and right through the cooperation of the transmission screw rod 604 and the threaded holes, and liquid outlet holes in the first extrusion cylinder 304 or the second extrusion cylinder 402 are combed through the brush wires 602 in the moving process, so that blockage is prevented.
In the screw press disclosed in this embodiment 3, when squeezing stevia rebaudiana leaves, an operator firstly inputs raw materials into the screw press through the feeding hopper 305, and synchronously drives the feeding screw 3071 and the tower-type variable diameter extrusion screw 3072 through the first driving device, wherein the feeding screw 3071 conveys the raw materials to the extrusion die 309, at this time, the extrusion die 309 is provided with the round table hole 3091 matched with the tower-type variable diameter extrusion screw 3072, effective juice in the stevia rebaudiana leaves is extruded and discharged once under the extrusion action of the two materials, the discharged juice is discharged through the liquid outlet, and the extrusion die 309 is connected with the sealing ring block 308 through the guide post and the adjusting spring 312, so that the extrusion die 309 has a certain retraction space, when the extrusion ratio is too large, the adjusting spring 312 can be compressed and moved outwards, and the material is prevented from heating too fast due to the too high extrusion ratio, and the effective components in the juice are prevented from deteriorating due to too high temperature.
Then, the materials discharged from the pulp extrusion die 309 enter the second extrusion barrel 402 through the U-shaped conveying pipe 500, and the materials are extruded by the variable-diameter variable-pitch extrusion spiral blades 406 in the second extrusion barrel 402 in the whole process, and the effective components in the stevia rebaudiana leaves are fully extruded in advance under the effective control of the extrusion temperature, so that the extraction rate of the stevia rebaudiana is greatly improved.
Example 4
Example 4 discloses the dimensioning of the screw press according to example 3, which is described in detail below with reference to fig. 7, 12.
The lowest end of the hopper 305 has an axial length A, the nominal radius of the feed screw 3071 is B, the pitch of the feed screw 3071 is C, the height of the hopper 305 is D, and the speed of the raw material reaching the lowest end of the hopper 305 is V 1 The rotational speed of the first driving device 306 is V n The axial feeding speed of the feeding screw 3071 is V 2
When meeting the requirementsDuring the process, the feeding screw blade 3071 is full of materials, so that the materials can be timely conveyed to the tower-type variable-diameter extrusion screw blade 3072, the materials can be fully extruded between the variable-diameter extrusion screw blade 3072 and the pulp extrusion die 309, the effective components in the materials can be fully extruded, and the extraction rate is improved.
Known to those skilled in the artV 2 =V n X C (3), which is obtainable by substituting into the formula 1, when satisfyingWhen the material is extruded fully between the variable diameter extrusion screw blade 3072 and the pulp extrusion die 309, the effective components in the material are extruded fully, and the extraction rate is improved.
Furthermore, as is clear from example 3, too large a squeeze ratio causes too fast a temperature rise of the material and causes deterioration of the active ingredients in the juice due to too high a temperature, and therefore, is preferableAt this time, the material can be fully extruded between the variable-diameter extrusion screw blade 3072 and the pulp extrusion die 309, so that the effective components in the material can be fully extruded, the extraction rate is improved, and meanwhile, the deterioration of the effective components in juice due to the over-high temperature caused by the over-high temperature of the material due to the over-high extrusion ratio is avoided.
The foregoing description of the preferred embodiments of the application is not intended to be limiting, but rather is intended to cover all modifications, equivalents, and alternatives falling within the spirit and principles of the application.

Claims (2)

1. A device for extracting high-activity stevioside, characterized in that the device is a screw press device, comprising a frame (100) and a receiving box (200);
a first screw press device (300) and a second screw press device (400) are arranged in parallel in front of and behind the upper end of the frame (100);
the ends of the same side of the first spiral pressing device (300) and the second spiral pressing device (400) are connected through a U-shaped conveying pipe (500);
the first screw press device (300) comprises a first press housing (301) fixedly mounted in a frame (100);
the lower end of the first press case (301) is connected with a first pulp guiding hopper (302) which is arranged at the left lower side and the right higher side, and the lower end of the first pulp guiding hopper (302) is connected with a first discharging pipe (303);
the material receiving box (200) is arranged right below the first material discharging pipe (303);
a first extrusion cylinder (304) is fixedly arranged in the first press case (301), a large number of liquid outlet holes are formed in the lower half part of the first extrusion cylinder (304), meanwhile, a feeding hopper (305) is connected to the upper surface of the left end of the first extrusion cylinder (304), and the feeding hopper (305) extends out of the upper surface of the first press case (301);
a sealing bearing is arranged on the left end face of the first extrusion barrel (304), a first driving device (306) is arranged on the left end face of the first extrusion machine box (301), and an output shaft of the first driving device (306) extends into the end part of the inner cavity of the first extrusion barrel (304) through the sealing bearing to be connected with a feeding spiral blade (3071);
the right end of the feeding spiral blade (3071) is connected with a tower-type variable-diameter extrusion spiral blade (3072);
a sealing ring block (308) is fixedly connected to the right end of the first extrusion barrel (304), a plurality of round holes (3081) are formed in the inner side surface of the sealing ring block (308) in an annular array shape, a pulp extrusion die head (309) is arranged at the right end of the inner cavity of the first extrusion barrel (304), the outer wall of the pulp extrusion die head (309) is attached to the inner cavity of the first extrusion barrel (304), round table holes (3091) with wide left and narrow right are formed in the pulp extrusion die head (309), a splicing pipe (310) extending out of the sealing ring block (308) is connected to the right end of the pulp extrusion die head (309), guide posts (311) inserted into the round holes (3081) are connected to the right end surface of the pulp extrusion die head (309), an adjusting spring (312) is sleeved on each guide post (311), and two ends of the adjusting spring (312) are respectively connected with the pulp extrusion die head (309) and the inner wall of the round holes (3081);
the round platform hole (3091) formed in the pulp extrusion die head (309) is matched with the tower-type variable-diameter extrusion spiral blade (3072);
the first screw press (300) is dimensioned to meetWherein the lowest axial length of the feeding hopper (305) is A, the nominal radius of the feeding spiral blade (3071) is B, the pitch of the feeding spiral blade (3071) is C, and the feeding hopper (305) is provided with a plurality of feeding groovesThe height is D, the rotating speed of the first driving device (306) is Vn, and the gravity acceleration is g;
the extraction method adopting the device comprises the following steps:
1) Weighing a certain amount of stevia rebaudiana leaves, drying the stevia rebaudiana leaves, crushing and sieving the dried stevia rebaudiana leaves for later use;
2) Soaking sieved stevia rebaudiana leaves in the extracting solution, fishing out the stevia rebaudiana leaves after soaking, putting the stevia rebaudiana leaves into the first spiral squeezing device (300) and the second spiral squeezing device (400) to sequentially perform primary squeezing and secondary squeezing, and mixing the squeezing solution obtained by the two squeezing steps with the soaking solution to obtain the extracting solution;
3) Centrifuging the obtained extract to remove impurities, and decolorizing and deodorizing the centrifugate after removing the impurities;
4) Sequentially washing columns and analyzing the decolorized and deodorized extracting solution, and concentrating the analyzing solution in vacuum;
5) Finally, spraying powder on the concentrated solution in a spray drying tower, and converting sugar solution into powdery solid to obtain high-activity stevioside; the water content of the dried stevia rebaudiana leaves in the step 1) is controlled to be 18-32%, and the mesh number of the dried stevia rebaudiana leaves She Guoshai is 100-200 meshes; the temperature of the vacuum concentration of the analytic liquid in the step 4) is controlled between 56 ℃ and 64 ℃.
2. Use of high-activity stevioside prepared by the extraction method using the device for high-activity stevioside extraction of claim 1 in food.
CN202211053297.9A 2022-08-31 2022-08-31 High-activity stevioside extraction method and application thereof Active CN115946387B (en)

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