CN115804384B

CN115804384B - Sterilization composition containing boscalid

Info

Publication number: CN115804384B
Application number: CN202211576881.2A
Authority: CN
Inventors: 郑敬敏; 周大伟; 刘欢
Original assignee: Agricultural Core Crop Technology Co ltd
Current assignee: Agricultural Core Crop Technology Co ltd
Priority date: 2022-12-09
Filing date: 2022-12-09
Publication date: 2024-05-31
Anticipated expiration: 2042-12-09
Also published as: CN115804384A

Abstract

The invention relates to a sterilization composition containing boscalid, which comprises boscalid and trioctyl benzene sulfonate as effective active components, wherein the mass ratio of the boscalid to the trioctyl benzene sulfonate in the composition is 20:1-1:20, preferably 10:1-1:10. The composition can be prepared into a plurality of agricultural allowable pesticide formulations, and the control effect is obviously improved compared with a single dose by binary compounding of the boscalid and the trioctyl benzene sulfonate, so that the pesticide dosage can be effectively reduced, the pesticide use times can be reduced, the cost can be reduced, and the generation of pathogenic bacteria drug resistance can be delayed.

Description

Sterilization composition containing boscalid

Technical Field

The invention relates to a sterilization composition, which consists of a first active ingredient of boscalid, a second active ingredient of biguanide trioctyl benzene sulfonate and an auxiliary agent, and belongs to the technical field of compound pesticides.

Background

The boscalid is an isoxazoline compound developed by Shenyang chemical industry institute, belongs to sterol synthesis inhibitor bactericides, has broad-spectrum bactericidal activity, has protection and treatment effects, has good systemic property, and can effectively control leaf diseases through root and leaf stem absorption. Can effectively prevent and treat gray mold, tomato leaf mold, wheat and cucumber powdery mildew, cucumber and pear scab, apple alternaria leaf spot, peanut brown spot, rice blast and the like of vegetables and fruits such as cucumber, tomato, leek, strawberry and the like.

The trioctyl benzene sulfonate, which is mainly used for the synthesis of lipid compounds of pathogenic bacteria and the cell membrane function, can inhibit spore germination, bud tube elongation, attached spore and hypha formation. The bactericidal spectrum is wider, and is used for preventing and treating diseases caused by ascomycetes and deuteromycetes. Can be widely used for preventing and treating diseases such as gray mold, powdery mildew, sclerotinia sclerotiorum, gummy stem blight, anthracnose, leaf spot, soft rot of fruits and vegetables of various crops caused by most ascomycetes and fungi imperfecti.

The long-term continuous high-dose application of single chemical bactericide is easy to cause the problems of medicament residue, environmental pollution, development of drug-resistant fungi and the like. The development of new bactericides has the positive characteristics of large investment, long time consumption, large development difficulty, and reasonable chemical bactericide compounding or compounding can improve the control effect, expand the bactericidal spectrum, reduce the dosage, reduce the cost, reduce the phytotoxicity, reduce the residue, delay the occurrence and development of fungus drug resistance and the like, so the reasonable compounding of bactericides is one of the most effective methods for solving the problems.

Disclosure of Invention

The invention aims to provide a reasonable compound sterilization composition, which can achieve synergistic effect through reasonable compounding, has good sterilization effect and low drug cost, and is safe to crops.

The technical scheme of the invention is as follows:

a) A first active ingredient: pyricularia oxazole

B) A second active ingredient: bis-trioctyl benzene sulfonate

The weight ratio of the first active ingredient to the second active ingredient is 20:1-1:20, preferably 10:1-1:10.

The sum of the contents of the first active ingredient and the second active ingredient is 1 to 90 percent of the total weight of the composition.

The bactericidal composition containing the boscalid can be prepared into various formulations which are allowed to be used in agriculture according to methods known by a person skilled in the art.

The pesticide composition of the invention is prepared into various application forms, and besides effective active ingredients, the pesticide composition also comprises various auxiliary ingredients which are allowed to be used and can be accepted in different pesticide forms.

The auxiliary components include, but are not limited to, emulsifying agents, dispersing agents, wetting agents, solvents, antifreezes, defoamers, thickeners, disintegrants, synergists, preservatives, fillers and the like, are components commonly used or allowed to be used in pesticide preparations, are not particularly limited, and specific components and the dosage are determined through experiments according to the requirements of the formula.

The emulsifier can be various emulsifiers known in the pesticide formulation field, and the emulsifier can be one or more of phenethyl phenol polyoxyethylene polyoxypropylene ether, alkylaryl polyoxyethylene polyoxypropylene ether, fatty acid polyoxyethylene ether, fatty alcohol polyoxyethylene ether, glycerol laurate polyoxyethylene ether, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol polyoxypropylene polyoxyethylene ether, styrylphenyl polyoxyethylene ether, alkylphenol polyoxyethylene ether, sorbitan fatty acid ester polyoxyethylene ether, fatty alcohol polyoxyethylene ether sulfonate, alkylphenol polyoxyethylene formaldehyde condensate, calcium dodecyl sulfonate, alkylphenol polyoxyethylene ether succinic monoester sulfonate, alkylphenol polyoxyethylene phosphate and alkylphenol polyoxyethylene ether sulfonated succinic acid.

The dispersant may be various dispersants known in the pesticide formulation field, and the dispersant may be one or more of alkylphenol ethoxylate phosphate, alkylphenol ethoxylate sulfate, phenethyl phenol ethoxylate phosphate, polyoxyethylene polyoxypropylene ether block copolymer, sodium lignin sulfonate, sodium methylenedinaphthyl sulfonate (dispersant NNO), calcium lignin sulfonate, polycarboxylate, sodium methylenedinaphthyl sulfonate, sodium methylenebis-methylnaphthalene sulfonate, polyvinylpyrrolidone, alkylamidyl taurate, sodium alkyl naphthalene sulfonate polycondensate, sodium methylenebis-naphthalene sulfonate formaldehyde condensate.

The wetting agent can be various wetting agents known in the pesticide preparation field, and the wetting agent can be one or more of sodium alkyl naphthalene sulfonate, sodium dodecyl sulfate, secondary sodium alkyl sulfate, sodium dodecyl benzene sulfonate, fatty alcohol polyglycol ether sulfate, fatty alcohol polyoxyethylene ether, nonylphenol polyoxyethylene ether, sorbitan fatty acid ester polyoxyethylene ether, alkyl naphthalene formaldehyde condensate, alkyl naphthalene sulfonate and other wetting agents.

The solvent may be any of various solvents known in the pesticide formulation field, and the solvent may be one or more of water, methanol, ethanol, N-methylpyrrolidone, N-dimethylformamide, N-dimethylacetamide, tetrahydrofurfuryl alcohol, toluene, xylene, dimethyl sulfoxide, tetrahydrofuran, cyclohexanone, N-butanol, isopropanol, solvent oil No. 100, no. 150, no. 180, and No. 200.

The antifreezing agent can be various antifreezing agents known in the pesticide preparation field, and can be one or more of ethylene glycol, propylene glycol glycerol, diethylene glycol, urea and inorganic salts.

The defoamer can be various defoamers known in the pesticide preparation field, and can be one or more of silicone compounds, epoxidized soybean oil, emulsified silicone oil and fatty alcohol.

The thickener may be various thickeners known in the pesticide formulation field, and the thickener may be one or more of xanthan gum, polyvinyl alcohol, sodium alginate, gum arabic, and magnesium aluminum silicate.

The disintegrating agent can be various disintegrating agents known in the pesticide preparation field, and the disintegrating agent can be one or more of ammonium sulfate, sodium carboxymethyl starch, urea, low-substituted hydroxypropyl cellulose, croscarmellose sodium, crospovidone, sodium alginate, sodium bicarbonate and sodium chloride.

The carrier can be various carriers known in the pesticide preparation field, and the carrier can be one or more of glucose, white carbon black, kaolin, diatomite, light calcium carbonate, heavy calcium carbonate, attapulgite, soluble starch, urea, maltose, sucrose, citric acid, potassium carbonate, sodium carbonate and anhydrous sodium sulfate.

The preservative may be any of a variety of preservatives known in the art of pesticide formulation, and may be one or more of kathon, sodium benzoate, benzoic acid, potassium sorbate, formaldehyde.

The deionized water is industrial deionized water.

The production process of various application forms of the pesticide composition belongs to the prior known technology and is not repeated here.

The invention relates to application of a bactericidal composition containing boscalid in preventing and treating plant disease fungi, wherein diseases caused by the plant disease fungi mainly comprise crop gray mold, powdery mildew, leaf mold, scab, early blight, glume blight, net blotch, rust disease, anthracnose, scab, gray star, black spot, ring spot, fallen leaf, gummy stem blight and the like.

The invention has the beneficial effects that:

1. according to the invention, the boscalid and the biguanide trioctyl benzene sulfonate are compounded, and the synergistic effect is obvious under a certain mixing proportion;

2. Compared with a single dose, the field control effect of the composition mixed by the boscalid and the trioctyl benzene sulfonate is obviously improved, the dosage of the medicament can be effectively reduced, and the cost is reduced;

3. the active ingredients of the invention, namely the boscalid and the trioctyl benzene sulfonate, have different action mechanisms, and after being mixed, the invention can effectively delay the generation of germ resistance, prolong the service life of the medicament and protect the environment.

Detailed Description

The technical scheme of the present invention is described below in connection with the specific embodiments, but the specific protection scope of the present invention is not limited to the following specific embodiments.

1. Indoor toxicity determination of Botrytis cinerea by compounding boscalid oxazole and biguanide trioctyl benzene sulfonate

The test method comprises the following steps: the test method is a hypha growth rate method.

First, a medicated plate PDA medium was prepared. Preparing the reagent to be tested into 5 concentration gradients, sucking the liquid medicine to be tested with different concentration gradients by a liquid-transferring gun, injecting the liquid medicine into a sterile culture dish, mixing the liquid medicine and the culture medium uniformly according to the volume ratio of 1:9, and preparing the tablet PDA culture medium containing the reagent, wherein each treatment is repeated for 3 times. Then, a puncher is used for punching bacterial cakes at the edge of a bacterial colony after 3d culture, the bacterial cakes are inversely placed in the center of a medicine-containing flat plate, and the flat plate bacterial cakes without adding medicines are used as a control for culture in a constant temperature cabinet at 25 ℃. After 3d, the colony diameter is measured by a crisscross method, and the inhibition rate of different medicaments and medicaments with different concentrations on the growth of germ hyphae is calculated.

The EC ₅₀ value of each drug was obtained by linear regression analysis between the several values of the inhibition rate and the logarithmic values of the series concentration, and the combined effect of the two drugs was evaluated by the grand-cloud-Pei co-toxicity coefficient method (CTC). The co-toxicity coefficient CTC of the compound preparation is more than or equal to 120 and shows synergistic effect; ctc.ltoreq.80 shows antagonism; 80 < CTC < 120 shows additive effect.

The calculation formula is as follows: antibacterial ratio = (control colony diameter-treated colony diameter)/control colony diameter x 100%

TABLE 1 determination of the Combined action of boscalid oxazole and Dizatrioctyl benzene sulfonate complex on Botrytis cinerea

As can be obtained from Table 1, the boscalid and the trioctyl benzene sulfonate are mixed according to the proportion of 20:1-1:20, a certain synergistic effect is shown on botrytis cinerea, and when the proportion of the boscalid and the trioctyl benzene sulfonate is 2:1, the co-toxicity coefficient is maximum, and the synergistic effect is most obvious.

For a better illustration of the invention, the following description of the invention will be given in connection with formulation examples, wherein the percentages in the formulation are by weight.

Formulation example 1

20% Of boscalid, 10% of biguanide trioctyl benzene sulfonate, 2.5% of sodium alkyl sulfate, 3% of sodium alkyl naphthalene sulfonate, 2% of bentonite, 3% of propylene glycol, 1.5% of isothiazolinone, 1% of butanol and deionized water to 100% by weight. The raw materials are mixed, sheared and dispersed for 30min at high speed, and sand ground by a sand mill to prepare the 30% boscalid-biguanide suspending agent.

Formulation example 2

15% Of boscalid, 5% of biguanide trioctyl benzene sulfonate, 3.6% of triphenyl ethylphenol polyoxypropylene polyoxyethylene block polymer, 3% of alkyl polyoxyethylene ether sodium sulfonate, 3% of magnesium aluminum silicate, 1.5% of carboxyethyl cellulose, 3% of polyethylene glycol, 1.5% of sodium benzoate, 1% of silicone oil and deionized water to 100% by weight. The raw materials are mixed, sheared and dispersed for 30min at high speed, and sand ground by a sand mill to prepare the 20% boscalid-biguanide suspending agent.

Biological example 1: and (5) field efficacy test for preventing and treating grape gray mold.

In 2022, field efficacy tests for preventing and controlling grape gray mold of preparation example 1 and preparation example 2 were carried out, and the field efficacy tests were experimentally set in a Lintong region of western An city of Shaanxi, so as to verify the prevention and control effect of each preparation on grape gray mold and the safety of each preparation on grape.

The test method comprises the following steps: 2 vines per cell are treated for 4 times, the cell groups are arranged randomly, and the application method adopts a conventional spraying method, so that the liquid medicine is uniformly sprayed on all leaves and fruits. The first application time is 2022, 6 months and 10 days, the second application is carried out after 7 days interval, the total application time is 2 times, and the control is treated by clear water.

The investigation method comprises the following steps: investigation was performed 7 days after the second administration and 14 days after the administration, respectively. All clusters of all 2 grapes are investigated in each district, and the total cluster number and the cluster number of each stage of diseases are recorded.

Disease ear grading standard:

level 0: the whole spike has no disease fruit;

stage 1: the number of the diseased fruits accounts for less than 5% of the whole cluster;

3 stages: the number of the diseased fruits accounts for 6% -15% of the whole cluster;

5 stages: the number of the diseased fruits accounts for 16% -25% of the whole cluster;

7 stages: the number of the diseased fruits accounts for 26-50% of the whole cluster;

Stage 9: the number of the diseased fruits accounts for more than 51% of the whole fruit cluster.

Disease index = [ Σ (number of ears at each stage×relative index) ]/(investigation of total number of ears×9) ×100

Control effect (%) = [ (control area disease index-treatment area disease index)/control area disease index ] ×100

TABLE 2 field test results for controlling grape gray mold

From Table 2, after the boscalid and the trioctyl benzene sulfonate are compounded, the grape gray mold can be effectively prevented and treated, the prevention effect after the medicine is over 88 percent in 7 days, and the prevention effect after the medicine is over 89 percent in 14 days, which indicates that the compounded medicine has good quick acting property, long lasting period and a certain synergistic effect, and the prevention effect is better than that of a single medicine.

Safety investigation shows that the target is not affected adversely in the range of the test drug.

Biological example 2: and (5) field efficacy test for preventing and treating tomato gray mold.

In 2022, field experiments for preventing and treating the gray mold of tomatoes in preparation example 1 and preparation example 2 are carried out in Wugong county of Shanxi province, and the prevention and treatment effect of the pesticide on the gray mold of tomatoes and the safety of tomatoes are verified.

The test method comprises the following steps: the test cells 15m ², each time 4 replicates are processed, the cell groups are randomly arranged. The application method adopts a conventional spraying method. The drug administration is started at the initial stage of the gray mold attack of the tomatoes, and is carried out once again at intervals of 7 days, and the drug administration is carried out three times.

The investigation method comprises the following steps: every 7 days and 14 days after the last medicine is investigated, 5 points are randomly taken for each cell, 2 plants are investigated for each point, all fruits are investigated for each plant, and the number of the fruits at each level is recorded.

Disease fruit classification standard:

level 0: no disease spots;

stage 1: onset of residual petals or onset of stigma;

3 stages: sepal rot or stigma disease spreads to the fruit umbilical region;

5 stages: the navel part is provided with infiltration spots but no mildew layer;

7 stages: the umbilicus has a mould layer but does not spread to other parts;

Stage 9: the umbilicus mould layer extends to other parts.

Disease index = [ Σ (number of disease fruits at each stage×number of relative stages)/(total fruits in investigation×9) ]×100

TABLE 3 field test results for controlling Botrytis cinerea

From Table 3, after the boscalid and the trioctyl benzene sulfonate are compounded, the tomato gray mold can be effectively prevented and treated, the prevention effect after the medicine is over 87 percent in 7 days, and the prevention effect after the medicine is over 89 percent in 14 days, which indicates that the compounded medicine has good quick acting property, long lasting period, better prevention effect than that of a single medicine and remarkable synergistic effect.

In conclusion, the bactericidal composition containing the boscalid and the trioctyl benzene sulfonate as effective active ingredients has remarkable synergism after being compounded, has good control effect on gray mold of crops, and has the advantages of good quick acting property, long lasting period, safety to target crops and no phytotoxicity in a use dosage range. The invention can effectively reduce cost and environmental pollution, and the invention and popularization of the compound preparation have very important significance to society.

Claims

1. The sterilizing composition containing the boscalid is characterized in that: the bactericidal composition comprises the active ingredients of the boscalid and the biguanide trioctyl benzene sulfonate, wherein the mass ratio of the boscalid to the biguanide trioctyl benzene sulfonate is 20:1-1:20, and the boscalid and the biguanide trioctyl benzene sulfonate account for 1-90% of the composition by weight percent.

2. The bactericidal composition containing boscalid according to claim 1, characterized in that: the mass ratio of the active ingredients of the boscalid to the biguanide trioctyl benzene sulfonate is 10:1-1:10.

3. The bactericidal composition containing boscalid according to claim 1, characterized in that: the bactericidal composition is in the form of suspending agent and wettable powder.

4. Use of a bactericidal composition containing boscalid as claimed in claim 1 for controlling phytopathogenic fungi.

5. Use of a bactericidal composition containing boscalid as claimed in claim 1 for controlling pathogenic fungi on cereals, fruit trees, vegetables, cash crops.

6. Use according to claim 4, characterized in that: the pathogenic fungi are fungi of the phylum Deuteromycotina.

7. Use according to claim 4, characterized in that: the application is to prevent and treat gray mold of crops.