CN114052032A - Bactericidal composition containing Dichlorobentazox and coumoxystrobin and application thereof - Google Patents
Bactericidal composition containing Dichlorobentazox and coumoxystrobin and application thereof Download PDFInfo
- Publication number
- CN114052032A CN114052032A CN202111449524.5A CN202111449524A CN114052032A CN 114052032 A CN114052032 A CN 114052032A CN 202111449524 A CN202111449524 A CN 202111449524A CN 114052032 A CN114052032 A CN 114052032A
- Authority
- CN
- China
- Prior art keywords
- coumoxystrobin
- bactericidal composition
- agent
- rice
- dichlobenizox
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a bactericidal composition containing dichlobenizox and coumoxystrobin and an application thereof, wherein the mass ratio of dichlobenizox to coumoxystrobin is 1: 50-50: 1. The composition has reasonable components, can effectively prevent and treat fungal diseases of rice, and has low dosage, wide bactericidal spectrum and safety to crops compared with the prior single preparation.
Description
Technical Field
The invention relates to the field of pesticide compounding, in particular to a bactericidal composition containing dichlobeniazox and coumoxystrobin and application thereof.
Background
Rice blast caused by ascomycete m.oryzae and rice sheath blight caused by r.solani are 2 important fungal diseases seriously harmful to rice production. The rice blast mainly damages leaves, stalks and ears of rice, and occurs in all rice areas in the world, thus seriously threatening the production and yield of the rice. The yield loss caused by global rice blast is 10-20% every year, and 40-50% of the yield loss can be caused in severe cases, even the yield loss is no more than zero. The rice sheath blight disease is one of the common diseases of rice, is widely distributed in various rice production areas in the world, and in recent years, due to the popularization of high-yield varieties, the use of a large amount of chemical fertilizers, the increase of rice planting area and the like, the occurrence of the rice sheath blight disease is increased year by year, so that 10-30% of yield loss can be generally caused, and the yield is reduced by 50% in case of high disease.
Dichlorobentazox having CAS number of 957144-77-3 and Chinese chemical name of 3- [ (3, 4-dichloro-1, 2-thiazol-5-yl) methoxy]-1, 2-benzothiazole 1, 1-dioxide with the molecular formula C11H6Cl2N2 O3 S2. Dichlobentiazox is a benzothiazole fungicide developed by Nippon combinatorial chemistry industries, and is mainly used for controlling rice diseases. Dichlobentizox structural formula is as follows:
coumoxystrobin with CAS accession number of 850881-70-8 and Chinese cultural name of (E) -2- [2- [ (3-butyl-4-methyl-2-oxobenzopyran-7-yl) oxymethyl]Phenyl radical]-3-methoxyprop-2-enoic acid methyl ester with molecular formula C26H28O6. Coumoxystrobin is a high-end bactericide developed by Shenyang chemical research institute, and Bada pesticide Limited company in Jilin province, belongs to methoxy acrylate, is a protective bactericide, and simultaneously has a certain therapeutic effect. Coumoxystrobin can block electron transfer between cytochrome b and cytochrome c, so that mitochondrial respiration is inhibited, ATP synthesis is hindered, fungi lack energy supply and gradually lose infection capacity. It has the features of broad spectrum, low toxicity, high efficiency and high safety, and has the functions of immunity, preventing and treating, and increasing yield and income. The special effect on the apple tree canker is the most authoritative medicament for preventing and treating the canker in China. The bactericidal spectrum is wide, and the bactericidal composition is effective on downy mildew, late blight, scab, anthracnose and leaf mold of melons, fruits, vegetables and fruit trees; meanwhile, the composition is also effective to ring spot, anthracnose, cotton wilt, rice plague, sheath blight, wheat root rot and corn small spot. The coumoxystrobin has the following structural formula:
in the agricultural production process, the application of chemical agents is the most effective means for preventing and treating plant diseases, but the problems of agent residue, environmental pollution, drug-resistant fungus development and the like are easily caused by continuously using a single chemical bactericide with high dose for a long time. The reasonable chemical bactericide mixing has the characteristics of reducing phytotoxicity, reducing pesticide residue, improving the control effect, delaying the occurrence of fungal drug resistance and the like. However, the research cost for developing new bactericides is high, and compared with the research cost, the compound bactericides have less investment. The invention provides a bactericidal composition containing Dichlorobentazox and coumoxystrobin on the basis of indoor screening and field tests, and the bactericidal composition has an obvious synergistic effect.
Disclosure of Invention
Based on the above situation, the invention provides a novel efficient bactericidal composition, which has good control effects on rice sheath blight and rice blast, is low in use cost, and can effectively delay the drug resistance of pathogenic fungi.
In order to achieve the purpose, the technical scheme of the invention is as follows: the bactericidal composition comprises the active ingredients of dichlobbentazox and coumoxystrobin, wherein the mass ratio of dichlobbentazox to coumoxystrobin is 1: 50-50: 1;
further, the mass ratio of dichlobeniazox to coumoxystrobin is 1: 10-10: 1;
furthermore, the mass ratio of dichlobeniazox to coumoxystrobin is 1:10, 1:5, 1:3, 1:1, 3:1, 5:1 and 10: 1;
further, the mass ratio of dichlobeniazox to coumoxystrobin is 1: 5-5: 1;
furthermore, the mass ratio of dichlobeniazox to coumoxystrobin is 1:5, 1:3, 1:1, 3:1, 5: 1;
further, the mass ratio of dichlobeniazox to coumoxystrobin is 1: 3-3: 1;
furthermore, the mass ratio of dichlobeniazox to coumoxystrobin is 1:3, 1:1, 3: 1;
further, the active ingredients dichlobenizox and coumoxystrobin account for 1-85% of the total mass of the bactericidal composition;
further, the bactericidal composition can be prepared into any preparation form acceptable in agriculture;
further, the dosage form includes any one of powder, granule, soluble powder, soluble granule, soluble tablet, water dispersible granule, wettable powder, microcapsule granule, water dispersible tablet, microcapsule suspension, dispersible liquid, emulsifiable concentrate, water aqua, emulsion in water, microemulsion, suspension, suspoemulsion, soluble liquid, and ultra-low volume liquid;
furthermore, the preparation form is any one of suspending agent, aqueous emulsion, microemulsion and missible oil;
furthermore, the bactericidal composition comprises other auxiliary components besides the effective components of dichlobeniazox and coumoxystrobin;
still further, the auxiliary ingredients include wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, antifoaming agents, solvents, preservatives, stabilizers, synergists, and carriers;
the wetting agent is selected from one or more of alkyl benzene sulfonate, alkyl naphthalene sulfonate, lignosulfonate, sodium dodecyl sulfate, dioctyl sodium sulfosuccinate, alpha-olefin sulfonate, alkylphenol polyoxyethylene, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm excrement, Chinese honeylocust fruit powder, soapberry powder, SOPA, detergent, emulsifier 2000 series or wetting penetrating agent F;
the dispersing agent is selected from one or more of lignosulfonate, alkyl naphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate ester, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylate, polyacrylic acid, phosphate, EO-PO block copolymer or EO-PO graft copolymer;
the emulsifier is selected from one or more of calcium dodecyl benzene sulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenethyl phenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styryl phenol polyoxyethylene ether, castor oil polyoxyethylene ether or alkylphenol ether phosphate;
the thickening agent is selected from one or more of xanthan gum, organic bentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose or white carbon black;
the disintegrating agent is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid or tartaric acid;
the antifreezing agent is selected from one or more of alcohols, alcohol ethers, chlorohydrocarbons or inorganic salts;
the defoaming agent is selected from C10-C20Saturated fatty acid compounds, silicone compounds or C8-C10One or more of fatty alcohols;
the solvent is selected from one or more of benzene, toluene, xylene, methanol, ethanol, isopropanol, N-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, N-methylpyrrolidone, alkylene carbonate, diesel oil, solvent oil, vegetable oil derivatives or deionized water;
the preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate or carbazone;
the stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2, 6-di-tert-butyl-p-cresol, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talcum powder, montmorillonite or starch;
the synergist is selected from synergistic phosphorus or synergistic ether;
the carrier is selected from one or more of ammonium salt, ground natural mineral, ground artificial mineral, silicate, resin, wax, solid fertilizer, water, organic solvent, mineral oil, vegetable oil or vegetable oil derivative.
An application of a bactericidal composition containing Dichlorobentazox and coumoxystrobin in preventing and treating fungal diseases of crops.
Further, the crop is rice.
Furthermore, the fungal diseases of the crops are rice blast and rice sheath blight.
A method for controlling fungal diseases of crops comprises the step of applying the bactericidal composition to a fungal disease occurrence place and/or a medium for pathogenic bacteria growth.
The sterilization composition has the beneficial effects that:
1. the bactericidal composition has obvious synergistic effect on the prevention and treatment of fungal diseases such as rice blast, rice sheath blight and the like, and the prevention and treatment effect is obviously improved;
2. compared with a single agent, the pesticide composition reduces the using amount and the using times of pesticides, reduces the prevention and treatment cost, and is environment-friendly;
3. the two active components are mixed to effectively control pathogenic microorganisms to generate drug resistance, and the service life of the medicament can be prolonged.
Detailed Description
To make the technical solutions, objects, and advantages of the present invention more apparent, the present invention is described with the following specific examples, but the present invention may be implemented in various forms and should not be limited by the embodiments set forth herein. The invention has been described in detail with respect to the specific embodiments and general description, but it will be apparent to those skilled in the art that various changes and modifications can be made without departing from the spirit and scope of the invention.
Preparation example 1: 10% dichlobenizox syringomycins emulsion in water
According to the weight percentage, 7.5 percent of Dichlorobentazox, 2.5 percent of coumoxystrobin, 6 percent of EO-PO block copolymer, 25 percent of cyclohexanone, 0.15 percent of xanthan gum, 3 percent of glycol, 3 percent of urea, 0.5 percent of sodium benzoate, 0.1 percent of organosilicon defoamer and deionized water to make up the balance.
The preparation method comprises the following steps: according to the formula design, a surfactant, an antifreezing agent, a density regulator and water are mixed into a water phase, then the raw medicines are dissolved by a solvent and added into the water phase under stirring, after uniform stirring, a thickening agent and a preservative are added and continuously sheared for 10min, and then a defoaming agent is added and uniformly stirred to form the O/W type aqueous emulsion.
Preparation example 2: 12.5% dichlobenizox syringomycinia microemulsion
According to the weight percentage, 7.5 percent of Dichlorobentazox, 5 percent of coumoxystrobin, 10 percent of styryl benzene polyoxyethylene ether, 5 percent of EO-PO block copolymer, 1 percent of fatty alcohol polyoxyethylene ether sodium sulfate, 25 percent of cyclohexanone, 5 percent of propylene glycol and 0.1 percent of organic silicon defoamer deionized water are added for the rest.
The preparation method comprises the following steps: according to the formula design, the effective components, the solvent, the emulsifier and the like are uniformly mixed to prepare an oil phase, the antifreezing agent and the water are uniformly mixed to prepare a water phase, the oil phase is added into the water phase under the stirring state and is uniformly stirred, the shearing is continued for 10min, the antifoaming agent is added and is uniformly stirred, and small liquid drops with the oil phase particles of 0.01-0.1 micron are obtained, so that the microemulsion is prepared.
Preparation example 3: 30% dichlobenizox syringyl ester suspension
According to the weight percentage, 15 percent of dichlobeniazox, 15 percent of coumoxystrobin, 1 percent of lignosulfonate, 1.5 percent of sodium dodecyl sulfate, 2 percent of EO-PO block copolymer, 3 percent of styryl phenol polyoxyethylene ether phosphate ester salt, 1 percent of magnesium aluminum silicate, 0.2 percent of xanthan gum, 0.5 percent of sodium sorbate, 6 percent of glycerol, 0.5 percent of organosilicon defoamer and deionized water are added for the rest.
The preparation method comprises the following steps: according to the formula design, deionized water, a dispersing agent and a wetting agent are uniformly mixed, a defoaming agent and an anti-freezing agent are sequentially added under the stirring state, the materials are uniformly sheared and mixed at a high speed, Dichlorobentazox and coumoxystrobin are added, the materials are continuously sheared and uniformly mixed, then the materials are ground in a horizontal sand mill, the particle size of the materials is reduced to below 5 mu m, a thickening agent is added, and the materials are sheared uniformly to obtain the suspending agent.
Preparation example 4: 30% Dichlobeniazox syringyl ester missible oil
According to the weight percentage, 7.5 percent of Dichlorobentazox, 22.5 percent of coumoxystrobin, 20 percent of cyclohexanone, 10 percent of N, N-dimethylformamide, 2 percent of calcium dodecyl benzene sulfonate, 8 percent of styrylphenol polyoxyethylene ether, 3 percent of castor oil polyoxyethylene ether and xylene are added for the rest.
The preparation method comprises the following steps: according to the formula design, the raw medicines are dissolved in an organic solvent, then an emulsifier is added, and the mixture is uniformly mixed under the condition of shearing and stirring to prepare uniform and transparent missible oil.
Indoor activity test
Example 1: combined toxicity test of Dichlobeniazox and coumoxystrobin mixture on rice blast
The test basis is as follows: reference is made to NY/T1156.2-2006 "indoor bioassay Standard for pesticides test guidelines section 2 for fungicides: the plate method of pathogenic fungus hypha growth inhibition test; NY/T1156.6-2006 agricultural chemical indoor bioassay test guidelines part 6 of fungicides: combined action assay of compounding.
Pathogenic bacteria to be tested: the rice blast (m.oryzae) was isolated, stored and purified by the group research and development center.
Reagent to be tested: 96% of coumoxystrobin raw drug and 92% of Dichlobentiazox raw drug, which are provided by the research and development center of the group.
Preparing a medicament: dissolving the raw materials with acetone, and diluting with 0.1% Tween-80 water solution, wherein each preparation has 5 mass concentration gradients.
Medicament treatment: under aseptic operation conditions, quantitatively adding the sterilized PDA culture medium which is melted in advance into an aseptic conical flask according to test treatment, quantitatively sucking liquid medicines from low concentration to high concentration in sequence, respectively adding into the conical flasks, and fully shaking up. Then, the mixture was poured into a petri dish with a diameter of 9cm in equal amount to prepare a drug-containing plate with a corresponding concentration.
The test was run with no agent treatment as a blank, with 4 replicates per treatment.
Inoculation: and (3) cutting off fungus cakes from the edges of bacterial colonies by using a 5mm sterilization puncher under aseptic conditions for about 5-7 days, inoculating the fungus cakes to the center of a drug-containing flat plate by using an inoculator, covering a dish cover, and placing the medicine-containing flat plate in an incubator at 25 ℃ for culturing for 5 days.
And (3) investigation: the diameter of the colonies was measured by a cross method with calipers, each colony was measured perpendicularly once, and the average value was taken for each treatment.
The data statistics and calculation method comprises the following steps: according to the investigation result, the hypha growth inhibition rate of each treatment concentration on the test target bacteria is calculated according to the following formula. The test adopts DPS data processing system to process the data, respectively establishes toxicity regression equation, and calculates the drug EC50Value and co-toxicity coefficient.
In the formula:
i, colony growth inhibition rate;
D0-blank control colony growth diameter;
dt-agent treatment colony growth diameter.
The synergistic effect of the mixed medicines is determined according to a Sun Yunpei co-toxicity coefficient (CTC) method, namely that the CTC is less than or equal to 80 and is antagonistic, the CTC is more than 80 and less than 120 and is additive, and the CTC is more than or equal to 120 and is synergistic. The co-toxicity coefficient was calculated according to the following formula:
in the formula:
ATI-actually measured virulence index of the combination;
S-EC of Standard drug50In milligrams per liter (mg/L);
M-EC of the test mixtures50In milligrams per liter (mg/L).
TTI＝TIA×PA+TIB×PB
In the formula:
TTI-theoretical virulence index of the mixture;
TIA-a agent virulence index;
PA-the percentage of agent a in the mixture in percent (%);
TIB-agent virulence index;
PBthe percentage of the agent B in the mixture is given in percentage (%).
In the formula:
CTC-co-toxicity coefficient;
ATI-actually measured toxicity index of mixed agent;
TTI-mixture theory virulence index.
The test results are shown in Table 1.
Table 1 shows the combined toxicity test results of blending Dichlobeniazox and coumoxystrobin with different ratios on rice blast germs
| Test reagent and proportion | Regression equation (Y ═) | Coefficient of correlation R | EC50(mg/L) | EC50Confidence interval | Co-toxicity coefficient |
| DichlobentiazoxA | y＝5.3779+1.3048x | 0.9924 | 0.5133 | 0.4454～0.5916 | - |
| Eugenia caryophyllata ester B | y＝4.7279+1.5776x | 0.9975 | 1.4875 | 1.3758～1.6083 | - |
| A:B＝1:50 | y＝4.7505+1.2660x | 0.9891 | 1.5741 | 1.1858～2.0896 | 91.1079 |
| A:B＝1:30 | y＝4.8502+1.0572x | 0.9933 | 1.3856 | 1.1243～1.7077 | 101.1608 |
| A:B＝1:10 | y＝4.9957+0.9697x | 0.9865 | 1.0104 | 0.7842～1.3018 | 125.5558 |
| A:B＝1:5 | y＝5.0755+0.9719x | 0.9885 | 0.8361 | 0.6759～1.0344 | 135.1567 |
| A:B＝1:3 | y＝5.1372+0.9048x | 0.9899 | 0.7053 | 0.5873～0.8471 | 143.0357 |
| A:B＝1:1 | y＝5.2642+0.8614x | 0.9965 | 0.4935 | 0.4489～0.5426 | 154.6562 |
| A:B＝3:1 | y＝5.3644+0.9653x | 0.9843 | 0.4192 | 0.3436～0.5116 | 146.4212 |
| A:B＝5:1 | y＝5.3955+1.0576x | 0.9973 | 0.4227 | 0.3895～0.4587 | 136.3127 |
| A:B＝10:1 | y＝5.3362+0.9408x | 0.9913 | 0.4392 | 0.3787～0.5093 | 124.2705 |
| A:B＝30:1 | y＝5.3276+1.0605x | 0.9882 | 0.4910 | 0.4120～0.5852 | 106.7980 |
| A:B＝50:1 | y＝5.2793+1.0471x | 0.9941 | 0.5410 | 0.4768～0.6140 | 96.1141 |
As can be seen from Table 1, Dichlobeniazox has high toxicity to Pyricularia oryzae, EC500.5133mg/L, the weight ratio of Dichlorobentazox to coumoxystrobin is in the range of 1: 50-50: 1, the co-toxicity coefficients are both more than 80, and the blending of Dichlorobentazox and coumoxystrobin in the range shows additive or synergistic effect, and no antagonistic effect occurs.
When the weight ratio of Dichlobeniazox to coumoxystrobin is in the range of 1: 10-10: 1, the co-toxicity coefficients are both greater than 120, which indicates that the Dichlobeniazox and coumoxystrobin have synergistic action in the weight ratio range; when the weight ratio of dichlobenizox to coumoxystrobin is 1: 5-5: 1, the co-toxicity coefficients are all larger than 130, which indicates that the synergism of dichlobenizox and coumoxystrobin is prominent in the weight range; wherein, when the weight ratio of Dichlorobentazox to coumoxystrobin is 1:1, the co-toxicity coefficient is 154.6562, and the synergistic effect is most remarkable.
Example 2: combined toxicity test of Dichlobeniazox and coumoxystrobin mixture on rice sheath blight disease
The test basis is as follows: reference is made to NY/T1156.2-2006 "indoor bioassay Standard for pesticides test guidelines section 2 for fungicides: the plate method of pathogenic fungus hypha growth inhibition test; NY/T1156.6-2006 agricultural chemical indoor bioassay test guidelines part 6 of fungicides: combined action assay of compounding.
Pathogenic bacteria to be tested: rice sheath blight bacterium (r. solani), isolated, stored and purified by group research and development centers.
Reagent to be tested: 96% of coumoxystrobin raw drug and 92% of Dichlobentiazox raw drug, which are provided by the research and development center of the group.
Preparing a medicament: dissolving the raw materials with acetone, and diluting with 0.1% Tween-80 water solution, wherein each preparation has 5 mass concentration gradients.
Medicament treatment: under aseptic operation conditions, quantitatively adding the sterilized PDA culture medium which is melted in advance into an aseptic conical flask according to test treatment, quantitatively sucking liquid medicines from low concentration to high concentration in sequence, respectively adding into the conical flasks, and fully shaking up. Pouring the mixture into a culture dish with the diameter of 9cm in equal amount to prepare a drug-containing plate with the corresponding concentration.
The test was run with no agent treatment as a blank, with 4 replicates per treatment.
Inoculation: cutting off bacterial cake from the edge of bacterial colony by using a 5mm sterilization puncher under aseptic condition, inoculating the bacterial cake to the center of a drug-containing flat plate by using an inoculator, covering a dish cover, and placing the flat plate in an incubator at 22 ℃ for culturing for 3 d.
And (3) investigation: the diameter of the colonies was measured with calipers by the cross method, each colony was measured perpendicularly once, and the average was taken for each treatment.
The data statistics and calculation method comprises the following steps: the same as in example 1.
The test results are shown in Table 2.
Table 2 results of the combined toxicity test of the mixture of Dichlorobentazox and coumoxystrobin with different ratios on Rhizoctonia solani
As can be seen from Table 2, Dichlobeniazox has high toxicity to Rhizoctonia solani, EC500.9471mg/L, EC of coumoxystrobin on Rhizoctonia solani50It was 1.2757 mg/L. When the weight ratio of Dichlorobentazox to coumoxystrobin is in the range of 1: 10-10: 1, the co-toxicity coefficient is larger than 120, which indicates that the Dichlorobentazox and coumoxystrobin have synergistic effect in the weight ratio range, wherein when the weight ratio of Dichlorobentazox to coumoxystrobin is 1:1, the co-toxicity coefficient is 186.6285, and the synergistic effect is most remarkable.
Test of field drug effect
Example 3: dichlobeniazox and coumoxystrobin mixed field pesticide effect test for preventing and treating rice blast
The test basis is as follows: refer to GB/T17980.19-2000 'pesticide field efficacy test criterion (I) that the bactericide prevents and treats the rice leaf diseases'.
Test subjects: rice blast of rice.
And (3) test crops: rice (hubei 17).
Test site: the paddy fields in Chongyang county of Hubei province have moderate fertility in test fields, and the conditions of crop varieties, planting periods, growth vigor, fertilizer and water management and the like in each test cell are basically consistent.
The test method comprises the following steps: the experiment starts to apply the pesticide at the early stage of rice blast attack, the pesticide application time is arranged at the tillering stage of the rice (1 time in 7-month middle 2020), and the pesticide application is carried out by uniformly spraying by using a Singapore Linong brand HD400 type sprayer in clear weather. The districts are arranged according to random block groups, and ridges are built among every two districts, every two districts are arranged in a random block modeEach treatment was repeated 4 times, 30m per cell2. Sampling is carried out at 5 points per cell, 50 plants are taken at each point, 250 plants are investigated in each cell, 2 leaves under the flag leaf and the flag leaf are investigated in each cell, and 3 leaves are investigated in each cell.
The diseases are classified according to the following classification method:
level 0: no disease;
level 1: the number of the leaf spots is less than 5, and the length is less than 1 cm;
and 3, level: 6-10 leaf spots, wherein the length of part of the spots is more than 1 cm;
and 5, stage: 11-25 leaf spots, wherein part of the spots are connected into pieces and account for 10-25% of the leaf area;
and 7, stage: the number of the disease spots on the leaves is more than 26, and the disease spots are connected into tablets and account for 26 to 50 percent of the area of the leaves;
and 9, stage: the scabs of the leaves are connected into pieces, which account for more than 50 percent of the area of the leaves or are dead completely.
Investigation time and number of times: and 7d and 15d after the medicine is applied, investigating the number of the diseased leaves in each cell, and calculating the disease index and the prevention and treatment effect.
Calculating disease index and prevention and treatment effect according to the following formula:
in the formula:
CK-index of disease after drug administration in placebo zone;
PT-index of disease after administration to the agent treatment area.
The test results are shown in Table 3.
Table 3 results of field pesticide effect test of Dichlorobentiazox and coumoxystrobin for preventing and treating rice blast
As can be seen from the results of the field efficacy test in Table 3, the control effects of different mixed agents of Dichlorobentazox and coumoxystrobin on rice blast are better than those of the control agent. 7 days after the pesticide is applied, the control effects of 10% dichlobenizox coumoxystrobin aqueous emulsion, 12.5% dichlobenizox coumoxystrobin microemulsion, 30% dichlobenizox coumoxystrobin suspending agent and 30% dichlobenizox coumoxystrobin missible oil are 80.83%, 81.64%, 82.54% and 78.81% respectively. 15 days after application, the compound combination of the 30 percent dichlobeniazox and coumoxystrobin suspending agent has the most prominent effect of preventing and treating rice blast, the prevention effect is 86.16 percent, and good persistence is shown.
Example 4: dichlobeniazox and coumoxystrobin mixed field pesticide effect test for preventing and treating rice sheath blight disease
The test basis is as follows: refer to GB/T17980.20-2000 'pesticide field efficacy test criterion (I) bactericide for preventing and treating rice sheath blight disease'.
Test subjects: rice sheath blight disease.
And (3) test crops: rice (indica you 016).
Test site: the Guangting township village in the Suzhou city facies city has moderate fertility of the test land, and the conditions of crop varieties, planting period, growth vigor, fertilizer and water management and the like of each test cell are basically consistent.
The test method comprises the following steps: the test was carried out in the test field in 8 months of 2020, using a Singapore Linong brand HD400 sprayer during application, in clear weather. The application is carried out 2 times (1 st spray in 8 middle-month day, 2 nd spray 8 days after the first application) with emphasis on the base of the stem. The cells are arranged in random blocks, ridges are built between every two cells, each treatment is repeated for 4 times, and each cell has a height of 30m2. Random sampling is carried out at 5 points per cell, random 6 clusters are investigated at each point, and 30 clusters are investigated in total per cell.
The disease condition was graded according to the following grading method:
level 0: the whole plant is disease-free;
level 1: the fourth leaf and the leaf sheaths and leaves below the fourth leaf (sword leaf is taken as the first leaf);
and 3, level: the third leaf and the leaf sheaths and leaves below the third leaf are attacked;
and 5, stage: the second leaf and the leaf sheaths and leaves below the second leaf are attacked;
and 7, stage: attack of Jianye leaf and the leaf sheaths and leaves below the Jianye leaf;
and 9, stage: the whole plant is attacked and withered early in advance.
The investigation method comprises the following steps: the test is carried out twice, the total plant number, the disease plant number and the disease stage number are respectively investigated and recorded 7 days after the first medicine and 10 days after the second medicine, and the disease index and the prevention and treatment effect are calculated.
Calculating disease index and prevention and treatment effect according to the following formula:
in the formula:
CK-index of disease after drug administration in placebo zone;
PT-index of disease after administration to the agent treatment area.
The test results are shown in Table 4.
TABLE 4 field efficacy test results of Dichlobeniazox and coumoxystrobin mixture for preventing and treating rice sheath blight disease
As can be seen from Table 4, the 30% Dichlobeniazox syringomycinum suspension has good control effect on rice sheath blight, and the dosage is 60g/hm at 7 days after the first application2、75g/hm2、90g/hm2The control effect of the 30 percent Dichlorobenthiazox syringomycins suspending agent on rice sheath blight disease is 78.70 percent respectively,80.37 percent and 83.96 percent, which are higher than the control effect of a single agent; the dosage is 60g/hm 10 days after the second time of administration2、75g/hm2、90g/hm2The control effects of the Dichlorobentazox and coumoxystrobin suspending agent on rice sheath blight are 83.82%, 85.68% and 89.07% respectively.
In conclusion, through indoor toxicity measurement and field efficacy tests, the bactericidal composition containing dichlobeniazox and coumoxystrobin has good control effects on rice blast and sheath blight of rice, is safe to target crops, has obvious control effects, is superior to a single agent in the aspects of delaying the generation of drug resistance and prolonging the persistence, and can effectively reduce the production cost and the use cost.
Claims (10)
1. The bactericidal composition containing dichlobenizox and coumoxystrobin is characterized in that the active ingredients of the bactericidal composition are dichlobenizox and coumoxystrobin, and the mass ratio of dichlobenizox to coumoxystrobin is 1: 50-50: 1.
2. The bactericidal composition according to claim 1, wherein the mass ratio of dichlobeniazox to coumoxystrobin is 1: 10-10: 1.
3. The bactericidal composition according to claim 1, wherein the mass ratio of dichlobeniazox to coumoxystrobin is 1: 5-5: 1.
4. The bactericidal composition of claim 1, wherein the active ingredients dichlobenizox and coumoxystrobin comprise 1-85% of the total weight of the bactericidal composition.
5. The bactericidal composition of claim 1, wherein the bactericidal composition is formulated into any agriculturally acceptable formulation.
6. The bactericidal composition of claim 1, wherein the bactericidal composition further comprises other auxiliary ingredients in addition to the active ingredients dichlobeniazox and coumoxystrobin;
the auxiliary components comprise a wetting agent, a dispersing agent, an emulsifying agent, a thickening agent, a disintegrating agent, an antifreezing agent, an antifoaming agent, a solvent, a preservative, a stabilizing agent, a synergist and a carrier.
7. An application of a bactericidal composition containing Dichlorobentazox and coumoxystrobin in preventing and treating fungal diseases of crops.
8. The use according to claim 7, wherein the crop is rice.
9. The use of claim 7, wherein the fungal diseases of crops are rice blast and rice sheath blight.
10. A method for controlling fungal diseases in crops, which comprises applying the composition of any one of claims 1 to 6 to the locus where the fungal disease occurs and/or to the medium in which the fungal pathogen grows.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111449524.5A CN114052032A (en) | 2021-11-30 | 2021-11-30 | Bactericidal composition containing Dichlorobentazox and coumoxystrobin and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111449524.5A CN114052032A (en) | 2021-11-30 | 2021-11-30 | Bactericidal composition containing Dichlorobentazox and coumoxystrobin and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114052032A true CN114052032A (en) | 2022-02-18 |
Family
ID=80228130
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111449524.5A Withdrawn CN114052032A (en) | 2021-11-30 | 2021-11-30 | Bactericidal composition containing Dichlorobentazox and coumoxystrobin and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114052032A (en) |
-
2021
- 2021-11-30 CN CN202111449524.5A patent/CN114052032A/en not_active Withdrawn
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104839168B (en) | Bactericidal composition and its application | |
| CN114617129B (en) | Sterilization composition and application thereof | |
| CN116076516A (en) | Mite-killing composition and application thereof | |
| CN114052032A (en) | Bactericidal composition containing Dichlorobentazox and coumoxystrobin and application thereof | |
| CN113966743A (en) | Bactericidal mixture containing succinate dehydrogenase inhibitor and application thereof | |
| CN114391545B (en) | Pesticide composition containing bixafen and copper quinolinate and application thereof | |
| CN114451416B (en) | Pesticide composition containing benzovindiflupyr and application thereof | |
| CN114766492B (en) | Pesticide composition containing bixafen and application thereof | |
| CN114766493B (en) | Sterilization composition containing agricultural antibiotics and application thereof | |
| CN114617128B (en) | Pesticide composition containing copper quinolinate and application thereof | |
| CN114747582B (en) | Pesticide composition containing Isoflucypram and copper quinolinate and application thereof | |
| CN112136819B (en) | Sterilization composition containing fenpicoxamid and penflufen | |
| CN112471162B (en) | Sterilization composition containing tetramycin and pyraclostrobin | |
| CN115251066B (en) | Sterilization composition containing cyprodinil | |
| CN114586789B (en) | Mite-killing composition and application thereof | |
| CN114586791B (en) | Sterilization composition containing fluxapyroxad and application thereof | |
| CN116349689B (en) | Acynonapyr-containing pesticide composition | |
| CN117322427A (en) | Use of benzovindiflupyr-containing bactericidal composition for preventing and controlling citrus anthracnose | |
| CN117958269A (en) | Sterilization composition containing prothioconazole and application thereof | |
| CN114158565A (en) | Bactericidal composition containing fenpyrazamine and chlorophenoxyacetic acid amide and application thereof | |
| CN117598312A (en) | Sterilization composition containing cyclopurifiuram and application thereof | |
| CN116391719A (en) | Sterilization composition and application thereof | |
| CN117981759A (en) | Cyclobutrifluram-containing sterilization composition and application thereof | |
| CN115804384A (en) | Bactericidal composition containing pyrisoxazole | |
| CN115474607A (en) | Fluoxaprirolin-containing bactericidal composition and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20220218 |
|
| WW01 | Invention patent application withdrawn after publication |