CN115671198A - Antiviral preparation for pigs and preparation method thereof - Google Patents
Antiviral preparation for pigs and preparation method thereof Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses an antiviral preparation for pigs and a preparation method thereof, wherein the antiviral preparation comprises the following components in parts by weight: 8-12 parts of wild chrysanthemum flower, 8-12 parts of fructus forsythiae, 8-10 parts of anise, 8-10 parts of garlic, 8-10 parts of buffalo horn, 5-8 parts of polyporus umbellatus, 5-8 parts of corydalis tuber, 5-8 parts of rhizoma atractylodis, 3-5 parts of salvia officinalis, 3-5 parts of caulis spatholobi, 3-5 parts of lactic acid bacteria, 3-5 parts of bacillus licheniformis, 3-5 parts of chitosan oligosaccharide and 10-20 parts of glucose. The invention has the advantages and beneficial effects that: (1) The application process is simple and is suitable for production; (2) The invention enhances the product effect and improves the bioavailability by applying the traditional Chinese medicine fermentation technology; (3) The mixture can effectively improve the immune function of the organism and prevent and control viral diseases; (4) The mixture of the invention is easy to be absorbed after entering the organism, and the clinical administration is convenient, thus being suitable for group administration and saving manpower and material resources.
Description
Technical Field
The invention belongs to the field of compound preparations, and particularly relates to an antiviral preparation for pigs and a preparation method thereof.
Background
The problem that viral diseases are troubled by farms all the time is that the prevention and treatment of the viral diseases are challenged by the forbidding of human antiviral drugs, and the problem that people pay attention to is how to make the antiviral drug which can well resist viruses, improve the immunity of organisms, reduce the occurrence of the viral diseases in the whole life process, and meanwhile, the antiviral drug does not harm the health of human beings all the time.
Wild chrysanthemum flower, being slightly cold in nature, has the effects of clearing away heat and toxic material, and relieving swelling and pain. Can be used for treating viral diseases such as influenza, epidemic cerebrospinal meningitis, skin sore, carbuncle, and pneumonia.
Lian Qiao, bitter in taste and slightly cold in nature, enters lung, heart and small intestine meridians. Clear heat and remove toxicity, resolve swelling and dissipate nodulation, disperse wind-heat. Can be used for treating wind-heat type common cold, pestilence, fever, sore, yellow and toxic swelling.
Garlic, pungent in flavor and wet in nature. It enters spleen, stomach and lung meridians. Regulate qi, stimulate appetite, remove toxicity, stop dysentery, kill parasites. Can be used for treating anorexia due to slow release of herbs, abdominal distention, dysentery, and abdominal pain due to parasitic infestation.
Buffalo horn is sweet in taste and cold in nature. It enters heart and liver meridians. Clear heat and arrest convulsion, cool blood and stop bleeding, remove toxicity. Can be used for treating hyperpyrexia, coma, mania, rash, hemorrhage, epistaxis, and hematochezia.
Polyporus umbellatus has sweet and light taste and mild property. It enters kidney and bladder meridians. Excrete dampness and induce diuresis. Can be used for treating dysuria, edema, diarrhea, stranguria with turbid urine, and leukorrhagia.
Yan Hu Suo is pungent, bitter and damp in flavor. It enters liver and spleen meridians. Promote blood circulation to dissipate blood stasis, promote qi circulation to relieve pain. Can be used for treating stagnation of qi and blood stasis, traumatic injury, puerperal stagnation, and rheumatalgia.
Cang Zhu is pungent, bitter and damp in flavor. It enters spleen, stomach and liver meridians. Dry dampness and invigorate spleen, dispel wind and cold, improve vision. Can be used for treating diarrhea, edema, arthralgia due to wind-cold-dampness, and common cold due to wind-cold-evil.
Salvia officinalis is bitter and pungent in flavor and mild in nature. Clearing away heat, promoting diuresis, promoting blood circulation, removing toxic substance and relieving swelling. Can be used for treating damp-heat, blood stasis, sore, yellow swelling, toxic materials, and traumatic injury.
Spatholobus stem, bitter and sweet in flavor, damp in nature. It enters liver and kidney meridians. Promoting blood circulation, tonifying blood and dredging collaterals. Can be used for treating flash injury, arthralgia due to wind-cold-dampness, strain, and blood deficiency.
Octagonal, warm in nature, pungent in flavor, enters liver, kidney, spleen and stomach meridians. Warm yang, dispel cold, regulate qi. It is indicated for vomiting due to middle cold, hernia of cold with abdominal pain, lumbago due to kidney deficiency.
The chitosan oligosaccharide has obvious effects of improving the immunity and the disease resistance of livestock, poultry and aquatic animals (fish, shrimp, shellfish and ginseng), promoting growth and the like. The chitosan oligosaccharide also has the function of preventing the growth and the reproduction of pathogenic bacteria, and can promote the synthesis of protein and the activation of cells, thereby improving the production performance of livestock and poultry.
The medicines aim to improve the immunity of the organism, strengthen the body resistance and eliminate evil according to the compatibility principle of monarch, minister, assistant and guide, thereby reducing the occurrence of virus diseases. The pharmacological activity of the traditional Chinese medicine is further activated by adopting a traditional Chinese medicine fermentation process, so that the effect of the medicine is enhanced. The raw materials are all safe, effective and residue-free green products, so that the feed is a good antiviral product for farmers.
Disclosure of Invention
One of the purposes of the invention is to provide an antiviral preparation for pigs.
The second purpose of the invention is to provide a preparation method of the compound preparation.
The invention also aims to provide the application of the compound preparation in viral diseases.
In order to achieve the purpose, the invention adopts the following technical scheme:
an antiviral preparation for pigs comprises the following components in percentage by weight: 8-12 parts of wild chrysanthemum flower, 8-12 parts of fructus forsythiae, 8-10 parts of anise, 8-10 parts of garlic, 8-10 parts of buffalo horn, 5-8 parts of polyporus umbellatus, 5-8 parts of corydalis tuber, 5-8 parts of rhizoma atractylodis, 3-5 parts of salvia officinalis, 3-5 parts of caulis spatholobi, 3-5 parts of lactic acid bacteria, 3-5 parts of bacillus licheniformis, 3-5 parts of chitosan oligosaccharide and 10-20 parts of glucose.
The preparation method comprises the following steps: cleaning wild chrysanthemum flower, forsythia, star anise, garlic, buffalo horn, polyporus, corydalis tuber, atractylodes rhizome, salvia and suberect spatholobus stem, drying, crushing, sieving by a 60-mesh sieve, adding glucose, lactic acid bacteria, bacillus licheniformis and chitosan oligosaccharide, uniformly mixing, adding a proper amount of rice washing water, uniformly kneading, placing in a fermentation tank, fermenting at the constant temperature of 35 +/-2 ℃ for 24-72 hours, taking out, and drying at the low temperature to obtain the product.
The preparation method comprises the following steps: (1) the dosage of the rice washing water is 2 times of that of the traditional Chinese medicine. Adding 1L of water into every 1kg of rice, soaking for two minutes, washing, and washing for three times by the method for every 1kg of rice to obtain the rice washing water used by the invention.
(2) The problem of mildew of the traditional Chinese medicine can be effectively solved by adopting a low-temperature drying method after fermentation.
(3) The low-temperature drying temperature is 50-55 deg.C.
The use method of the feed additive comprises the following steps: mixing and feeding the feed for 1 day 1 time and for 7 days, wherein 300kg of the feed is mixed in each 1kg of preparation.
The invention has the advantages and beneficial effects that: (1) The application process is simple and is suitable for production; (2) The invention enhances the product effect and improves the bioavailability by applying the traditional Chinese medicine fermentation technology; (3) The mixture can effectively improve the immune function of the organism and prevent and control viral diseases; (4) The mixture of the invention is easy to be absorbed after entering the organism, and the clinical administration is convenient, thus being suitable for group administration and saving manpower and material resources.
Detailed Description
The present invention will be further described below by way of examples, but the scope of the present invention is not limited thereto.
EXAMPLE 1 preparation of antiviral preparation for pig
An antiviral preparation for pigs comprises the following components in percentage by weight: 8 parts of wild chrysanthemum flower, 12 parts of weeping forsythia, 8 parts of star anise, 10 parts of garlic, 8 parts of buffalo horn, 8 parts of polyporus, 5 parts of corydalis tuber, 8 parts of atractylodes rhizome, 3 parts of sage, 5 parts of suberect spatholobus stem, 3 parts of lactic acid bacteria (the viable count of the lactic acid bacteria is more than or equal to 200 hundred million CFU/g, the same below), 5 parts of bacillus licheniformis (the viable count of the bacillus licheniformis is more than or equal to 100 hundred million CFU/g, the same below), 3 parts of chitosan oligosaccharide and 14 parts of glucose.
The preparation method comprises the following steps: cleaning wild chrysanthemum flower, forsythia, star anise, garlic, buffalo horn, polyporus, corydalis tuber, atractylodes rhizome, salvia and suberect spatholobus stem, drying, crushing, sieving by a 60-mesh sieve, adding glucose, lactic acid bacteria, bacillus licheniformis and chitosan oligosaccharide, uniformly mixing, adding a proper amount of rice washing water, uniformly kneading, placing in a fermentation tank, fermenting at the constant temperature of 35 +/-2 ℃ for 48 hours, taking out, and drying at the low temperature of 50 ℃ to obtain the product.
The preparation method comprises the following steps: the amount of the rice washing water is 2 times of the mass of the traditional Chinese medicines (the traditional Chinese medicines refer to the sum of the mass of the wild chrysanthemum flower, the fructus forsythiae, the aniseed, the garlic, the buffalo horn, the umbellate pore fungus, the corydalis tuber, the rhizoma atractylodis, the salvia officinalis and the suberect spatholobus stem).
The use method of the invention comprises the following steps: mixing and feeding the feed for 1 day 1 time and for 7 days, wherein 300kg of the feed is mixed with 1kg of the feed.
EXAMPLE 2 preparation of antiviral preparation for pig
An antiviral preparation for pigs comprises the following components in percentage by weight: 12 parts of wild chrysanthemum flower, 8 parts of weeping forsythia, 10 parts of star anise, 8 parts of garlic, 10 parts of buffalo horn, 5 parts of polyporus, 8 parts of corydalis tuber, 5 parts of atractylodes rhizome, 5 parts of sage, 3 parts of suberect spatholobus stem, 5 parts of lactic acid bacteria, 3 parts of bacillus licheniformis, 5 parts of chitosan oligosaccharide and 13 parts of glucose.
The preparation method comprises the following steps: cleaning wild chrysanthemum flower, forsythia, star anise, garlic, buffalo horn, polyporus, corydalis tuber, atractylodes rhizome, salvia and suberect spatholobus stem, drying, crushing, sieving by a 60-mesh sieve, adding glucose, lactic acid bacteria, bacillus licheniformis and chitosan oligosaccharide, uniformly mixing, adding a proper amount of rice washing water, uniformly kneading, placing in a fermentation tank, fermenting at the constant temperature of 35 +/-2 ℃ for 48 hours, taking out, and drying at the low temperature of 55 ℃ to obtain the product.
The use method of the invention comprises the following steps: mixing and feeding the feed for 1 day 1 time and for 7 days, wherein 300kg of the feed is mixed with 1kg of the feed.
EXAMPLE 3 preparation of antiviral preparation for pig
An antiviral preparation for pigs comprises the following components in percentage by weight: 10 parts of wild chrysanthemum flower, 10 parts of weeping forsythia capsule, 9 parts of star anise, 9 parts of garlic, 9 parts of buffalo horn, 6 parts of polyporus umbellatus, 7 parts of corydalis tuber, 6 parts of atractylodes rhizome, 4 parts of sage, 4 parts of spatholobus stem, 4 parts of lactic acid bacteria, 4 parts of bacillus licheniformis, 4 parts of chitosan oligosaccharide and 14 parts of glucose.
The preparation method comprises the following steps: cleaning wild chrysanthemum flower, forsythia, star anise, garlic, buffalo horn, polyporus, corydalis tuber, atractylodes rhizome, salvia and suberect spatholobus stem, drying, crushing, sieving by a 60-mesh sieve, adding glucose, lactic acid bacteria, bacillus licheniformis and chitosan oligosaccharide, uniformly mixing, adding a proper amount of rice washing water, uniformly kneading, placing in a fermentation tank, fermenting at the constant temperature of 35 +/-2 ℃ for 48 hours, taking out, and drying at the low temperature of 50 ℃ to obtain the product.
The preparation method comprises the following steps: the amount of the rice washing water is 2 times of the mass of the traditional Chinese medicine.
The using method of the invention comprises the following steps: mixed feeding, mixing 300kg of feed every 1kg, 1 time for 1 day, and continuously using for 7 days.
EXAMPLE 4 preparation of antiviral preparation for pig
An antiviral preparation for pigs comprises the following components in percentage by weight: 10 parts of wild chrysanthemum flower, 10 parts of weeping forsythia capsule, 9 parts of star anise, 9 parts of garlic, 9 parts of buffalo horn, 6 parts of polyporus umbellatus, 7 parts of corydalis tuber, 6 parts of atractylodes rhizome, 4 parts of sage, 4 parts of spatholobus stem, 4 parts of lactic acid bacteria, 4 parts of bacillus licheniformis, 4 parts of chitosan oligosaccharide and 14 parts of glucose.
The preparation method comprises the following steps: cleaning wild chrysanthemum flower, forsythia, star anise, garlic, buffalo horn, grifola, corydalis tuber, rhizoma atractylodis, salvia and suberect spatholobus stem, drying, crushing, sieving by a 60-mesh sieve, adding glucose, lactic acid bacteria, bacillus licheniformis and chitosan oligosaccharide, uniformly mixing, adding a proper amount of water, uniformly kneading, placing in a fermentation tank, fermenting at the constant temperature of 35 +/-2 ℃ for 48 hours, taking out, drying at the low temperature of 50 ℃ and obtaining the product of the invention.
The preparation method of the product comprises the following steps: the amount of water is 2 times of the mass of the traditional Chinese medicine.
The using method of the product comprises the following steps: mixed feeding, mixing 300kg of feed every 1kg, 1 time for 1 day, and continuously using for 7 days.
EXAMPLE 5 preparation of antiviral preparation for pig
An antiviral preparation for pigs comprises the following components in percentage by weight: 10 parts of wild chrysanthemum flower, 10 parts of weeping forsythia, 9 parts of star anise, 9 parts of garlic, 9 parts of buffalo horn, 6 parts of polyporus, 7 parts of corydalis tuber, 6 parts of atractylodes rhizome, 4 parts of sage, 4 parts of suberect spatholobus stem, 4 parts of lactic acid bacteria, 4 parts of bacillus licheniformis, 4 parts of chitosan oligosaccharide and 14 parts of glucose.
The preparation method comprises the following steps: cleaning flos Chrysanthemi Indici, fructus forsythiae, fructus Anisi Stellati, bulbus Allii, cornu Bubali, polyporus, rhizoma corydalis, rhizoma Atractylodis, herba Salvia officinalis, and caulis Spatholobi, drying, pulverizing, sieving with 60 mesh sieve, adding glucose, lactobacillus, bacillus licheniformis, and chitosan oligosaccharide, and mixing well to obtain the product.
The use method of the product comprises the following steps: mixed feeding, mixing 300kg of feed every 1kg, 1 time for 1 day, and continuously using for 7 days.
Example 6 comparative test of the preparation of the invention against antibodies to hog cholera
The method comprises the following steps:
(I) drugs and reagents
Test groups 1, 2, 3: the compound preparations prepared in examples 1, 2 and 3;
control group 1: the combination prepared in example 4;
control group 2: example 5 the Compound preparation
(II) test method
(1) And (3) experimental design: 100 piglets with similar gestational times and weights and the same blood relationship are selected as test animals and divided into 5 groups of 20 animals according to a random block design, wherein 3 test groups and 2 control groups are provided.
The application methods of the test medicines are as follows: mixed feeding is carried out, 300kg of the feed is stirred every 1kg of the feed, and the feed is mixed 1 time every 1 day.
The measures of free ingestion, epidemic prevention and management are consistent with the daily measures. The specific method is that the administration is started 5 days before the immunization of the pig according to the required dosage, and 5 days of continuous administration is a period. The medicine is continuously applied for 5 days before the second immunization.
The swine fever antibody level is detected by collecting blood at 15 days (before immunization), 30 days (after immunization) and 70 days (before secondary immunization) of piglets by adopting an indirect hemagglutination reaction method.
The blood coagulation titer reaches 1: above 16 are qualified for immunization, 1: the immunization was rejected at 16 or less.
(2) Results and analysis
TABLE 1 detection results of swine fever antibody level
As can be seen from table 1, the level of the maternal antibody in the 5 groups is between 1. The antibody level is detected for the first time after immunization, the antibody levels are in an ascending trend, the test groups 1, 2 and 3 are obviously improved, and the antibody level is higher and reaches more than 1. In the second detection, the antibody level is increased to different degrees. The compound preparation has obvious promotion effect on the improvement of the swine fever antibody.
Example 7 Effect on porcine epidemic diarrhea
The method comprises the following steps:
(I) drugs and reagents
Test groups 1, 2, 3: the compound preparations prepared in examples 1, 2 and 3;
control group 1: the combination prepared in example 4;
control group 2: the combination prepared in example 5;
(II) test method
(1) The affected pigs of 30-50 days old were selected and randomly divided into 5 groups of 20 pigs each, of which 3 test groups and 2 control groups.
The main symptoms of the sick pigs are watery diarrhea, or vomit during diarrhea, the sick pigs have normal or slightly high body temperature, depression, anorexia or abominability after eating, and the sick pigs are diagnosed with epidemic diarrhea.
The adding method comprises the following steps:
test group 1: mixing and feeding, wherein 300kg of mixed feed is added per 1kg of mixed feed;
test group 2: mixing and feeding, wherein 300kg of mixed feed is added per 1kg of mixed feed;
test group 3: mixing and feeding, wherein 300kg of the feed is mixed every 1 kg;
control group 1: mixing and feeding, wherein 300kg of the feed is mixed every 1 kg;
control group 2: mixing and feeding, wherein 300kg of the feed is mixed every 1kg of the feed.
Blood was collected 1 week and 2 weeks after feeding, respectively, and the test for epidemic diarrhea virus was performed, and the results are shown in table 2.
TABLE 2 negative outcome of epidemic diarrhea virus
From the above table it can be seen that: the test groups 1, 2 and 3 have obvious inhibiting effect on the epidemic diarrhea virus, the negative conversion rate in 1 week is averagely 61.7 percent, which is respectively 16.7 percent and 31.7 percent higher than the negative conversion rates of the control groups 1 and 2, the negative conversion rate in 2 weeks is averagely 81.7 percent, and which is respectively 16.7 percent and 36.7 percent higher than the negative conversion rate of the control group 1.
It can be seen from examples 6 and 7 that the effect of the product prepared by the traditional Chinese medicine fermentation process is obviously improved, and the rice washing water fermentation process adopted in the traditional Chinese medicine fermentation process of the invention also has great advantages, so that the curative effect of the medicine can be obviously improved, and the rice washing water contains nutrients such as protein, starch, mineral substances and the like, so that the spleen and stomach can be nurtured.
Claims (6)
1. An antiviral preparation for pigs is characterized by comprising the following components in parts by weight: 8-12 parts of wild chrysanthemum flower, 8-12 parts of fructus forsythiae, 8-10 parts of anise, 8-10 parts of garlic, 8-10 parts of buffalo horn, 5-8 parts of polyporus umbellatus, 5-8 parts of corydalis tuber, 5-8 parts of rhizoma atractylodis, 3-5 parts of salvia officinalis, 3-5 parts of caulis spatholobi, 3-5 parts of lactic acid bacteria, 3-5 parts of bacillus licheniformis, 3-5 parts of chitosan oligosaccharide and 10-20 parts of glucose.
2. The method for preparing the antiviral preparation for swine according to claim 1, wherein the process comprises: taking wild chrysanthemum flower, weeping forsythia, star anise, garlic, buffalo horn, polyporus, corydalis tuber, rhizoma atractylodis, salvia and suberect spatholobus stem, cleaning, drying, crushing, sieving with a 40-80-mesh sieve, adding glucose, lactic acid bacteria, bacillus licheniformis and chitosan oligosaccharide, uniformly mixing, adding a proper amount of rice washing water, uniformly kneading, placing in a fermentation tank, fermenting at the constant temperature of 35 +/-2 ℃ for 24-72 hours, taking out, and drying at a low temperature to obtain the product.
3. The method for preparing the preparation for swine antivirus according to claim 2, wherein the amount of the rice-washing water is 2 times of the mass of the traditional Chinese medicine.
4. The method for preparing the antiviral preparation for swine as claimed in claim 2, wherein the temperature of the low-temperature drying is 50-55 ℃.
5. The method of using the antiviral preparation for swine according to claim 1, wherein the feed is mixed with 300kg of feed per 1kg of the preparation.
6. The use of the antiviral preparation for swine according to claim 1 for swine antiviral.
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