CN115569235A - 一种基于脂质润滑的水凝胶的制备方法 - Google Patents
一种基于脂质润滑的水凝胶的制备方法 Download PDFInfo
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- CN115569235A CN115569235A CN202210753436.2A CN202210753436A CN115569235A CN 115569235 A CN115569235 A CN 115569235A CN 202210753436 A CN202210753436 A CN 202210753436A CN 115569235 A CN115569235 A CN 115569235A
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- hydrogel
- lipid
- lubrication
- dimethyl sulfoxide
- monomer
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Abstract
本发明公开了一种基于脂质润滑的水凝胶的制备方法,以高分子聚合物和聚乙烯醇为原料,以脂质为润滑剂,采用溶解‑冷冻‑盐析法制备出具有超强力学性能和低摩擦性能的水凝胶。本发明通过二甲基亚砜/水作为混合溶剂,使聚乙烯醇的分子链聚集效果更佳,并通过盐析效应来大大增强水凝胶的力学性能。所述水凝胶中含有低浓度的脂质体,可以形成一种可自我更新的脂质体边界层,降低水凝胶的摩擦系数,解决了现有聚乙烯醇水凝胶在仿生关节软骨材料上的强度不足以及无法自润滑的问题。
Description
技术领域
本发明涉及生物医学仿生材料制备的技术领域,更具体地,涉及一种基于脂质润滑的水凝胶的制备方法。
背景技术
关节软骨是关节骨末端的***,在有效吸收负载能量、减轻负荷、保护关节骨免受损伤、减少骨关节运动之间的摩擦方面起着重要作用。但由于其几乎无血管的性质和自愈能力有限,关节软骨在损伤后很难修复,如果没有及时治疗,甚至会导致骨关节损伤或骨关节炎,且随着时间的推移,损伤会越来越严重。目前人工关节置换术是临床治疗关节疾病的有效方法之一,常用的人工关节软骨材料有金属材料、陶瓷材料和高分子材料等。但金属材料易腐蚀;陶瓷材料韧性低、脆性大、容易断裂;高分子材料如聚乙烯硬度偏低,抗蠕变性能差,易在体内的滑动产生磨损的碎片,造成不同的生理和组织反应。因此,同时具备超高力学性能,低摩擦性能等优点的仿生关节软骨材料是在生物医学工程中急需的。
水凝胶具有非常优异的力学性能,近年来受到化学、材料、医学、生物、环境等领域的极大重视。水凝胶具有与天然关节软骨组织相似的的三维多孔网络结构,较高的含水量,良好的渗透性,以及较低的摩擦系数。如CN201710389381.0聚乙烯醇水凝胶及其制备方法公开其以聚乙烯醇为基质,以琼脂糖为成孔剂,在将聚乙烯醇完全溶于去离子水后,加入琼脂糖,混合均匀后,对混合液进行脱泡、冷冻、解冻和洗脱工艺后,制备得到兼具高强度和多孔结构的聚乙烯醇水凝胶。所述的聚乙烯醇水凝胶可以用于人工关节软骨材料,但是该方法制备的聚乙烯醇水凝胶在力学性能和稳定性上还是略有不足。
并且,仿生关节软骨材料不仅需要具有一定的力学强度,可以在运动过程中承受着巨大的压力,而且仿生关节软骨材料需要仿照人体关节软骨的自润滑机理调控高强度水凝胶材料的表面的润滑性能,使其润滑的保护下,降低材料表面的摩擦系数,不容易受到损伤。边界润滑是摩擦副的表面没有被液体层隔开的情况下,通过分子级别的边界膜支撑载荷的润滑机制。我们可以通过模拟这种边界润滑在水凝胶中加入脂质来实现自润滑。CN201711162920.3一种具有剪切力响应自润滑仿生关节软骨的制备方法,该方法是在高强度水凝胶材料中掺杂剪切力响应的超分子凝胶体系,一方面高强度水凝胶致密的化学交联网络保证了材料良好的力学强度,可以用来模仿关节软骨抗压缩承重的特性;另一方面剪切力响应的超分子水凝胶可发生凝胶-溶胶转换的相态变化,使材料在保持高强度的同时可以自主调控表面润滑性能,降低界面摩擦系数,进而实现对人关节软骨自润滑功能的模仿。但是,该方法是采用渗出润滑机理,通过施加法向载荷力时关节软骨内润滑基质渗出到软骨表面形成润滑层来达到润滑的效果,其对外界剪切力的依赖程度高,且剪切力响应存在不稳定的缺点,导致润滑效果差。
发明内容
本发明要解决的技术问题是针对现有聚乙烯醇水凝胶的强度不足以及自润滑能力差的不足,提供一种基于脂质润滑的水凝胶的制备方法。
本发明的目的通过以下技术方案予以实现:
一种基于脂质润滑的水凝胶的制备方法,步骤包括:
S1.将脂质分散至去离子水中,配置成脂质体分散液;
S2.在步骤S1中的脂质体分散液中加入二甲基亚砜,并将单体一和单体二溶解至含有二甲基亚砜的脂质体分散液中,搅拌均匀得到混合溶液,反应形成均相溶液;
S3.将反应后的均相溶液低温冷冻,然后将得到的冷冻水凝胶浸泡在盐溶液中后,最后将水凝胶浸泡在去离子水中,通过溶剂交换除去二甲基亚砜,得到基于脂质润滑的仿生关节软骨水凝胶。
进一步地,所述脂质包括磷脂酰乙醇胺、磷脂酰甘油、甘油糖脂、二磷脂酰甘油、磷脂酰肌醇、磷脂酰胆碱、神经鞘磷脂、肉豆蔻酸、鞘糖脂、软脂酸、棕榈油酸、油酸、磷脂酰丝氨酸、胆固醇、维生素、固醇、胆酸、天然色素、香精油、磷脂酰丝氨酸、鞘氨醇的一种或多种,所述脂质作为仿生关节软骨材料的主要润滑物质,通过其头基的高度水合作用提供润滑来减小水凝胶的摩擦系数,且随着水凝胶的磨损,它不断释放新的脂质来重建脂质边界层。优选地,所述脂质为磷脂酰胆碱脂质。
进一步地,所述脂质体分散液中脂质的浓度为30~60mM/L。优选地,所述脂质体分散液中脂质的浓度为45mM/L。
进一步地,所述脂质通过超声分散至去离子水中。优选地,所述分散在30℃下超声15min。
进一步地,所述单体一包括明胶、卡拉胶、壳聚糖、羧甲基壳聚糖、琼脂、琼脂糖、纤维素、羧甲基纤维素钠、海藻酸钠、透明质酸、环糊精、Pluronic F-127、淀粉、结冷胶的一种。单体一通过氢键作用可形成网络,这样大大提高了水凝胶的力学性能。优选地,所述单体一选择琼脂糖。所述琼脂糖在提高聚乙烯醇水凝胶的基础上还能增强水凝胶与细胞的粘附,提高聚乙烯醇水凝胶的生物相容性。
进一步地,所述单体二包括丙烯酰胺、甲基丙烯酸羟乙酯、羟乙基丙烯酰胺、聚乙烯醇、聚乙二醇、聚丙烯酸、甲基丙烯酸十八脂、单宁酸、植酸钠、腐殖酸、N,N-二甲基丙烯酰胺的一种,且单体一和单体二不相同。优选地,所述单体一选择聚乙烯醇。
进一步地,所述脂质体分散液与二甲基亚砜的质量比为1∶2~6,二甲基亚砜氧原子上的孤对电子可以与水形成强的分子间氢键,一个二甲基亚砜分子可以与两个水分子结合,通过控制水和二甲基亚砜的比例,可以使聚乙烯醇分子链聚集效果更好,形成微晶进行物理交联,提高聚乙烯醇的力学强度。
优选地,所述脂质体分散液与二甲基亚砜的质量比为1∶4。
进一步地,所述均相溶液中单体一的质量比浓度为1~10%。优选地,所述均相溶液中单体一的质量比浓度为2.5%。
进一步地,所述均相溶液中单体二的质量比浓度为10~25%。优选地,所述均相溶液中单体二的质量比浓度为18%。
进一步地,步骤S2中所述反应的温度为50~100℃,反应时间为1~5h。优选地,所述反应的温度为95℃,反应时间为3h。
进一步地,步骤S2中所述低温冷冻的温度为-30~-10℃,时间为8~16h。优选地,所述低温冷冻的温度为-20℃,时间为12h。
进一步地,所述盐溶液包括氯化钠、氯化铁、氯化亚铁、柠檬酸钠、柠檬酸铁、硫酸铁、硫酸亚铁、硝酸钠、氯化铵、碳酸钠、碳酸氢钠、高锰酸钾、过硫酸钾的一种或多种。优选地,所述盐溶液为柠檬酸钠。
进一步地,所述盐溶液的质量比浓度为5~50%。优选地,所述盐溶液的质量比浓度为20%。
根据上述基于脂质润滑的水凝胶的制备方法得到的水凝胶在仿生关节软骨中的应用。
与现有技术相比,有益效果是:
本发明所述方法主要以聚乙烯醇为基质,通过琼脂糖的氢键作用形成网络增强水凝胶的强度,同时利用二甲基亚砜氧原子上的孤对电子可以与水形成强的分子间氢键,通过控制水和二甲基亚砜的比例,可以使聚乙烯醇分子链聚集效果更好,形成微晶进行物理交联,进一步提高水凝胶的强度。通过实验发现,当水凝胶压缩至75%时,力学强度可以达到5.5MPa,断裂拉伸应变为1447%,拉伸强度为10MPa,撕裂能高达21104J/m2,远远高于关节软骨的撕裂能(~1000J/m2),能够满足关节软骨的力学性能要求。
本发明所述的水凝胶内部还含有脂质体,在滑动摩擦时环境中的水分子会因为固有的偶极矩而吸附在脂质体的磷脂酰胆碱头基上,形成水化层。而水化层不仅空间稳定性很高,拥有较大的承载能力,而且水化层中的水分子与其他的自由水分子可以很快的交换,从而保持快速的松弛效能,这样在面对载荷时,表现出更好的流体效应和更小的摩擦系数。所以利用脂质体的磷脂酰胆碱头基的高度水合作用提供润滑来减小水凝胶的摩擦系数,且随着水凝胶的磨损,它不断释放新的脂质来重建脂质边界层。通过实验得到水凝胶的摩擦系数为0.027,极大的降低了水凝胶的摩擦,避免了仿生关节软骨材料的表面损伤。
在高强度水凝胶材料中掺杂剪切力响应的超分子凝胶体系,一方面高强度水凝胶致密的化学交联网络保证了材料良好的力学强度,可以用来模仿关节软骨抗压缩承重的特性;另一方面剪切力响应的超分子水凝胶可发生凝胶-溶胶转换的相态变化,使材料在保持高强度的同时可以自主调控表面润滑性能,降低界面摩擦系数,进而实现对人关节软骨自润滑功能的模仿。但是该方法通过渗出润滑机理,依赖于外界力响应,且剪切力响应存在不稳定的缺点。
本发明所述制备方法简单,反应条件温和,无苛刻的实验条件限制,为仿生关节软骨水凝胶在生物医学方面的应用提供条件。
附图说明
图1是水凝胶的制备过程和形成机理示意图;
图2是实施例5制备水凝胶的SEM图;
图3是实施例5制备水凝胶中脂质体的SEM图;
图4是水凝胶的压缩应力-应变图;
图5是水凝胶的撕裂能图;
图6是水凝胶的摩擦系数图。
其中,图4和图5中所示A2.5P10为实施例1采用2.5%(w/w)琼脂糖和10%(w/w)的聚乙烯醇制备的仿生关节软骨水凝胶;A2.5P14为实施例4采用2.5%(w/w)琼脂糖和14%(w/w)的聚乙烯醇制备的仿生关节软骨水凝胶;A2.5P18为实施例5采用2.5%(w/w)琼脂糖和18%(w/w)的聚乙烯醇制备的仿生关节软骨水凝胶。
具体实施方式
下面结合实施例进一步解释和阐明,但具体实施例并不对本发明有任何形式的限定。若未特别指明,实施例中所用的方法和设备为本领常规方法和设备,所用原料均为常规市售原料。
实施例1
本实施例提供一种基于脂质润滑的水凝胶的制备方法,步骤包括:
S1.将二肉豆蔻酰磷脂酰胆碱脂质加入至去离子水中,在30℃下超声15min分散,配置成45mM/L的脂质体分散液;
S2.在步骤S1中的脂质体分散液中加入二甲基亚砜,其中脂质体分散液与二甲基亚砜的质量比为1:4。然后加入2.5%(w/w)琼脂糖和10%(w/w)的聚乙烯醇至含有二甲基亚砜的脂质体分散液中,搅拌均匀得到混合溶液,在95℃下反应形成均相溶液;
S3.将反应后的均相溶液在-20℃的环境中冷冻12h,然后将得到的冷冻水凝胶浸泡在20%(w/w)柠檬酸钠溶液中1.5h后,最后将水凝胶浸泡在去离子水中,通过溶剂交换除去二甲基亚砜,得到基于脂质润滑的仿生关节软骨水凝胶。
实施例2
本实施例提供一种基于脂质润滑的水凝胶的制备方法,步骤包括:
S1.将二肉豆蔻酰磷脂酰胆碱脂质加入至去离子水中,在30℃下超声15min分散,配置成45mM/L的脂质体分散液;
S2.在步骤S1中的脂质体分散液中加入二甲基亚砜,其中脂质体分散液与二甲基亚砜的质量比为1:4。然后加入5%(w/w)琼脂糖和10%(w/w)的聚乙烯醇至含有二甲基亚砜的脂质体分散液中,搅拌均匀得到混合溶液,在95℃下反应形成均相溶液;
S3.将反应后的均相溶液在-20℃的环境中冷冻12h,然后将得到的冷冻水凝胶浸泡在20%(w/w)柠檬酸钠溶液中1.5h后,最后将水凝胶浸泡在去离子水中,通过溶剂交换除去二甲基亚砜,得到基于脂质润滑的仿生关节软骨水凝胶。
实施例3
本实施例提供一种基于脂质润滑的水凝胶的制备方法,步骤包括:
S1.将二肉豆蔻酰磷脂酰胆碱脂质加入至去离子水中,在30℃下超声15min分散,配置成45mM/L的脂质体分散液;
S2.在步骤S1中的脂质体分散液中加入二甲基亚砜,其中脂质体分散液与二甲基亚砜的质量比为1:4。然后加入7.5%(w/w)琼脂糖和10%(w/w)的聚乙烯醇至含有二甲基亚砜的脂质体分散液中,搅拌均匀得到混合溶液,在95℃下反应形成均相溶液;
S3.将反应后的均相溶液在-20℃的环境中冷冻12h,然后将得到的冷冻水凝胶浸泡在20%(w/w)柠檬酸钠溶液中1.5h后,最后将水凝胶浸泡在去离子水中,通过溶剂交换除去二甲基亚砜,得到基于脂质润滑的仿生关节软骨水凝胶。
实施例4
本实施例提供一种基于脂质润滑的水凝胶的制备方法,步骤包括:
S1.将二肉豆蔻酰磷脂酰胆碱脂质加入至去离子水中,在30℃下超声15min分散,配置成45mM的脂质体分散液;
S2.在步骤S1中的脂质体分散液中加入二甲基亚砜,其中脂质体分散液与二甲基亚砜的质量比为1:4。然后加入2.5%(w/w)琼脂糖和14%(w/w)的聚乙烯醇至含有二甲基亚砜的脂质体分散液中,搅拌均匀得到混合溶液,在95℃下反应形成均相溶液;
S3.将反应后的均相溶液在-20℃的环境中冷冻12h,然后将得到的冷冻水凝胶浸泡在20%(w/w)柠檬酸钠溶液中1.5h后,最后将水凝胶浸泡在去离子水中,通过溶剂交换除去二甲基亚砜,得到基于脂质润滑的仿生关节软骨水凝胶。
实施例5
本实施例提供一种基于脂质润滑的水凝胶的制备方法,步骤包括:
S1.将二肉豆蔻酰磷脂酰胆碱脂质加入至去离子水中,在30℃下超声15min分散,配置成45mM/L的脂质体分散液;
S2.在步骤S1中的脂质体分散液中加入二甲基亚砜,其中脂质体分散液与二甲基亚砜的质量比为1:4。然后加入2.5%(w/w)琼脂糖和18%(w/w)的聚乙烯醇至含有二甲基亚砜的脂质体分散液中,搅拌均匀得到混合溶液,在95℃下反应形成均相溶液;
S3.将反应后的均相溶液在-20℃的环境中冷冻12h,然后将得到的冷冻水凝胶浸泡在20%(w/w)柠檬酸钠溶液中1.5h后,最后将水凝胶浸泡在去离子水中,通过溶剂交换除去二甲基亚砜,得到基于脂质润滑的仿生关节软骨水凝胶。
实施例6
本实施例提供一种基于脂质润滑的水凝胶的制备方法,步骤包括:
S1.将二肉豆蔻酰磷脂酰胆碱脂质加入至去离子水中,在30℃下超声15min分散,配置成45mM的脂质体分散液;
S2.在步骤S1中的脂质体分散液中加入二甲基亚砜,其中脂质体分散液与二甲基亚砜的质量比为1:2。然后加入2.5%(w/w)琼脂糖和18%(w/w)的聚乙烯醇至含有二甲基亚砜的脂质体分散液中,搅拌均匀得到混合溶液,在95℃下反应形成均相溶液;
S3.将反应后的均相溶液在-20℃的环境中冷冻12h,然后将得到的冷冻水凝胶浸泡在20%(w/w)柠檬酸钠溶液中1.5h后,最后将水凝胶浸泡在去离子水中,通过溶剂交换除去二甲基亚砜,得到基于脂质润滑的仿生关节软骨水凝胶。
实施例7
本实施例提供一种基于脂质润滑的水凝胶的制备方法,步骤包括:
S1.将二肉豆蔻酰磷脂酰胆碱脂质加入至去离子水中,在30℃下超声15min分散,配置成45mM/L的脂质体分散液;
S2.在步骤S1中的脂质体分散液中加入二甲基亚砜,其中脂质体分散液与二甲基亚砜的质量比为1:6。然后加入2.5%(w/w)琼脂糖和18%(w/w)的聚乙烯醇至含有二甲基亚砜的脂质体分散液中,搅拌均匀得到混合溶液,在95℃下反应形成均相溶液;
S3.将反应后的均相溶液在-20℃的环境中冷冻12h,然后将得到的冷冻水凝胶浸泡在20%(w/w)柠檬酸钠溶液中1.5h后,最后将水凝胶浸泡在去离子水中,通过溶剂交换除去二甲基亚砜,得到基于脂质润滑的仿生关节软骨水凝胶。
对比例1
本实施例提供一种基于脂质润滑的水凝胶的制备方法,步骤包括:
S1.将二肉豆蔻酰磷脂酰胆碱脂质加入至去离子水中,在30℃下超声15min分散,配置成45mM的脂质体分散液;
S2.在步骤S1中的脂质体分散液中加入2.5%(w/w)琼脂糖和18%(w/w)的聚乙烯醇和去离子水,搅拌均匀得到混合溶液,在95℃下反应形成均相溶液;
S3.将反应后的均相溶液在-20℃的环境中冷冻12h,然后将得到的冷冻水凝胶浸泡在20%(w/w)柠檬酸钠溶液中1.5h后,得到基于脂质润滑的仿生关节软骨水凝胶。
对比例2
S1.在去离子水中加入二甲基亚砜,其中去离子水与二甲基亚砜的质量比为1:4。然后加入2.5%(w/w)琼脂糖和18%(w/w)的聚乙烯醇至含有二甲基亚砜的去离子水中,搅拌均匀得到混合溶液,在95℃下反应形成均相溶液;
S2.将反应后的均相溶液在-20℃的环境中冷冻12h,然后将得到的冷冻水凝胶浸泡在20%(w/w)柠檬酸钠溶液中1.5h后,最后将水凝胶浸泡在去离子水中,通过溶剂交换除去二甲基亚砜,得到仿生关节软骨水凝胶。
实验例
1.电镜检测
由图2-图3可知,本发明所述方法制备的基于脂质润滑的仿生关节软骨水凝胶呈现致密的多孔结构,且其孔径较为均匀,其孔洞内分布有脂质体,不断释放新的脂质来重建脂质边界层,从而降低了水凝胶的摩擦,避免了仿生关节软骨材料的表面损伤。
2.力学性能检测
将实施例1~7及对比例1~2制备的基于脂质润滑的水凝胶进行力学性能检测,将水凝胶在装有1000N测压元件的万能试验机上分别进行速率为5mm/min的压缩实验和100mm/min的拉伸实验,实验结果如下表1:
表1
将实施例1~7对比例1~2制备的基于脂质润滑的水凝胶进行撕裂性能检测,将裤型水凝胶样品的一个臂夹紧并固定,而另一臂以100mm/min的速度夹紧并向上拉。实验结果如下表2:
表2
由表1、表2和图4、图5可知,本发明制备的水凝胶当水凝胶压缩至75%时,力学强度可以达到5.5MPa,断裂拉伸应变为1447%,拉伸强度为10MPa,可以满足关节软骨的性能要求,且撕裂能最高达21104J/m2,远远高于关节软骨的撕裂能(~1000J/m2)。
3.摩擦性能检测
将实施例1~7对比例1~2制备的基于脂质润滑的水凝胶进行摩擦性能检测,采用直径为10mm的不锈钢钢球做摩擦副,与水凝胶表面接触,设置相应的摩擦测试参数:摩擦温度T,载荷F,摩擦速率v和摩擦时间t分别为37℃,5N,1mm/s和30min,得到的摩擦系数如表3所示:
表3
摩擦系数 | |
30min | |
实施例1 | 0.021 |
实施例2 | 0.028 |
实施例3 | 0.022 |
实施例4 | 0.021 |
实施例5 | 0.027 |
实施例6 | 0.025 |
实施例7 | 0.027 |
对比例1 | 0.023 |
对比例2 | 0.053 |
将实施例5和对比例2制备的仿生关节软骨水凝胶分别在载荷5N,摩擦速率1mm/s,摩擦时间30min、载荷30N,摩擦速率1mm/s,摩擦时间30min和载荷5N,摩擦速率0.1mm/s,摩擦时间30min的条件下分别检测其摩擦性能,其性能结果如图6所示。
表4
摩擦系数 | |
5N-1mm/s-DMPC | 0.027 |
5N-1mm/s | 0.053 |
30N-1mm/s-DMPC | 0.109 |
30N-1mm/s | 0.126 |
5N-0.1mm/s-DMPC | 0.030 |
5N-0.1mm/s | 0.034 |
由表3、表4和图6可知,本发明所述方法制备的仿生关节软骨水凝胶中的脂质体能够提供润滑来减小水凝胶的摩擦系数。
显然,本发明的上述实施例仅仅是为清楚地说明本发明所作的举例,而并非是对本发明的实施方式的限定。对于所属领域的普通技术人员来说,在上述说明的基础上还可以做出其它不同形式的变化或变动。这里无需也无法对所有的实施方式予以穷举。凡在本发明的精神和原则之内所作的任何修改、等同替换和改进等,均应包含在本发明权利要求的保护范围之内。
Claims (10)
1.一种基于脂质润滑的水凝胶的制备方法,其特征在于,步骤包括:
S1.将脂质分散至去离子水中,配置成脂质体分散液;
S2.在步骤S1中的脂质体分散液中加入二甲基亚砜,并将单体一和单体二溶解至含有二甲基亚砜的脂质体分散液中,搅拌均匀得到混合溶液,反应形成均相溶液;
S3.将反应后的均相溶液低温冷冻,然后将得到的冷冻水凝胶浸泡在盐溶液中,最后将水凝胶浸泡在去离子水中,通过溶剂交换除去二甲基亚砜,得到基于脂质润滑的水凝胶。
2.根据权利要求1所述基于脂质润滑的水凝胶的制备方法,其特征在于,所述脂质包括磷脂酰乙醇胺、磷脂酰甘油、甘油糖脂、二磷脂酰甘油、磷脂酰肌醇、磷脂酰胆碱、神经鞘磷脂、肉豆蔻酸、鞘糖脂、软脂酸、棕榈油酸、油酸、磷脂酰丝氨酸、胆固醇、维生素、固醇、胆酸、天然色素、香精油、磷脂酰丝氨酸、鞘氨醇的一种或多种。
3.根据权利要求1所述基于脂质润滑的水凝胶的制备方法,其特征在于,所述单体一包括明胶、卡拉胶、壳聚糖、羧甲基壳聚糖、琼脂、琼脂糖、纤维素、羧甲基纤维素钠、海藻酸钠、透明质酸、环糊精、Pluronic F-127、淀粉、结冷胶的一种;所述单体二包括丙烯酰胺、甲基丙烯酸羟乙酯、羟乙基丙烯酰胺、聚乙烯醇、聚乙二醇、聚丙烯酸、甲基丙烯酸十八脂、单宁酸、植酸钠、腐殖酸、N,N-二甲基丙烯酰胺的一种,且单体一和单体二不相同。
4.根据权利要求1所述基于脂质润滑的水凝胶的制备方法,其特征在于,所述脂质体分散液中脂质的浓度为30~60mM。
5.根据权利要求1所述基于脂质润滑的水凝胶的制备方法,其特征在于,所述脂质体分散液与二甲基亚砜的质量比为1∶2~6。
6.根据权利要求1所述基于脂质润滑的水凝胶的制备方法,其特征在于,所述均相溶液中单体一的质量比浓度为1~10%;所述均相溶液中单体二的质量比浓度为10~25%。
7.根据权利要求1所述基于脂质润滑的水凝胶的制备方法,其特征在于,步骤S2中所述反应的温度为50~100℃,时间为1~5h。
8.根据权利要求1所述基于脂质润滑的水凝胶的制备方法,其特征在于,步骤S3低温冷冻的温度为-30~-10℃,时间为8~16h。
9.根据权利要求1所述基于脂质润滑的水凝胶的制备方法,其特征在于,所述盐溶液包括氯化钠、氯化铁、氯化亚铁、柠檬酸钠、柠檬酸铁、硫酸铁、硫酸亚铁、硝酸钠、氯化铵、碳酸钠、碳酸氢钠、高锰酸钾、过硫酸钾的一种或多种;所述盐溶液的质量比浓度为5~50%。
10.根据权利要求1所述基于脂质润滑的水凝胶的制备方法得到的水凝胶在仿生关节软骨中的应用。
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