CN1154802A - Enayme-decomposed whole egg product, preparation method and use thereof - Google Patents
Enayme-decomposed whole egg product, preparation method and use thereof Download PDFInfo
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Abstract
The present invention relates to an enzymolytic whole egg product, including solution and powder. It is made up by using egg white and egg yellow, and making them pass through the process of enzymolysis treatment by using complex enzyme. Said invented product not only contains various small-molecular peptides and amino acids which are easilyabosrbed by human body, but also remains several vitamins and nutrients contained in whole egg, so that it can be used as a health-care product. Its preparation process is simple and easy, and its cost is low.
Description
The present invention relates to a kind of enayme-decomposed whole egg product, be to adopt the mixed enzyme hydrolysis to comprise the technology of the shell egg of egg white and yolk, this whole egg product can further be mixed with oral liquid, capsule, beverage and other nutritional health food.
In recent years, research to the natural activity peptide has obtained great progress, its quantity has reached thousands of kinds, because it has biological specificity, most of peptides all have important physical effect (Peter, Karlson, 1984, Bichemie III) glutathione can be treated hepatopathy and medicine and gestosis as is known, and can be anti-aging in advance; Five enkephalins have analgesic activity, and thymopeptide-5 can be treated children's primary immunodeficiency and some cancer etc.; Oxytocins can promote uterine contractile and galactogogue action when childbirth; Nonapeptide and sleep inducing peptide can make because of the anxiety insomniac and recover normal; The decapeptide gonadotropin releasing hormone can be used to treat gynaecological disease and sterility etc.According to reports, extracting about amino acid and multiple peptide mixer from soybean, is the nutrient protein source of weight, is again the important function raw-food material, and it is digested and assimilated, and can promote the physiologically active of the useful bacterium of human body and grows.The Tian Jizheng of Kyoto Univ Japan is bright to use enzyme incision technology to rice, and the pentapeptide of extraction can promote secretion of insulin, also has to extract to bring high blood pressure down or strengthen immune peptide (Chinese food, on October 4th, 1,996 three editions).Amino acid as component peptide also has various important physical functions, and amino acid can and be transported to liver by the absorption of gastrodermis cell, may synthesize oneself protein matter in liver, also may carry out catabolism.And for example, aspartic acid can be treated liver diseases, and its sylvite has step-down and diuresis; Threonine can be controlled hypoferric anemia; Histidine can be controlled gastroenteritic ulcer, anaemia and arthritis; Lysine is except that promoting to grow, but also eliminate-poverty blood and blood ammonia disease; Proline energy accelerating wound and treatment chronic hepatitis; Leucine can promote growth; Glycine can be treated muscle weakness etc.Small-molecular peptides and amino acid whose mixture, its absorptivity and infiltration rate in human body is good than simple amino acid all; Low molecular peptide can also be protected easy ruined amino acid in enteron aisle.In addition, simple amino acid is reformulated peptide and protein in vivo as required, and small-molecular peptides and amino acid whose mixture can save the process that a part reconfigures, so its biological value is higher.
Usually can produce required peptide with artificial synthesis or by genetic engineering, but difficulty height, expense are big, and prepare various peptide matters with enzymolysis protein matter, it is a kind of ideal method, start from the forties abroad, domesticly begin to report to some extent in the sixties, but used be fibrin, casein and vegetable protein etc.Yellow Yongchang etc. had once been reported with the method for papain enzymolysis ovalbumin (ovalbumin) and products thereof (CN93112571.5), but can't carry out enzymolysis to yolk.The nutrition of birds, beasts and eggs is quite abundant, and it contains rich in protein, fat, vitamin, mineral matter and trace element.These nutriments all are absolutely necessary to growing and having good health and a long life of human body, and the nutritional drugs egg also has the effect of disease preventing and treating.With the egg is example, and its protein is that quality, kind and composition are optimum in all food, and its AAS is 100.The contained protein of egg accounts for 10～12% in egg white, yellowish-white based on the white of an egg; In yolk, account for 15～17%, based on phosphoprotein, in egg white, fat and mineral matter trace.And fat accounts for 30% in yolk, and containing in the fat must aliphatic acid, lecithin and cholesterol etc., these all be human body grow and metabolism institute requisite; And mineral matter, trace element and the various vitamins except that vitamin C nearly all concentrate in the yolk.Taking a broad view of the contained various nutriments of egg white and yolk relatively, be not difficult to find out, the utilization of birds, beasts and eggs is not utilized the higher yolk of nutritive value if only be conceived to egg white, then is very unfortunate and unscientific.So people are still constantly seeking various easy methods, figure for and have amino acid and peptide and health food thereof active, that be easy to absorption of human body.
The purpose of this invention is to provide a kind of enayme-decomposed whole egg product, this goods are a kind of enayme-decomposed whole egg solution or dry powder, contain amino acid and peptide that human body easily absorbs, also can contain the trace element of needed by human body.
Another object of the present invention provides a kind of method for preparing above-mentioned enayme-decomposed whole egg product, promptly uses the method for mixed enzyme enayme-decomposed whole egg.
The present invention also has a purpose to provide the purposes of this enayme-decomposed whole egg product.
Enayme-decomposed whole egg product provided by the invention contains small-molecular peptides and comprises the pure-natural biological material of the several amino acids of taurine, and the weight ratio of amino acid and water is 0.014～0.8: 1～0.Can be enayme-decomposed whole egg solution and enayme-decomposed whole egg dry powder, its weight ratio can be respectively 0.014～0.8: 1 and 0.014～0.8: 0～0.05.Water content is decided by drying means in the enayme-decomposed whole egg dry powder.
Contain organic nitrogen 0.2～15% in the enayme-decomposed whole egg product of the present invention.Containing organic nitrogen in the enayme-decomposed whole egg solution is 0.2～1.5%, and containing organic nitrogen in the enayme-decomposed whole egg dry powder is 4～15%.
Contain organic matter molecular mass in the enayme-decomposed whole egg product of the present invention and be distributed as 50000～30, recommending molecular weight is 10000～40, and wherein molecular weight＞1000 is 20～50%, molecular weight＜1000 be 80～50%.
Typical organic matter molecular mass distributes as shown in Figure 1 in the enayme-decomposed whole egg product of the present invention.
It is as shown in table 1 to contain amino acid composition in the enayme-decomposed whole egg product of the present invention, and amino acid content is 14～80mg/ml in the enayme-decomposed whole egg solution.
Also can contain microelements of calcium, phosphorus, iron, zinc and iodine in the enayme-decomposed whole egg product of the present invention.In enayme-decomposed whole egg solution, calcium content is 180～720mg/l, and phosphorus content is 160～980mg/l, and iron content is 10～100mg/l, and zinc content is 4～20mg/l, and content of iodine is 1～50mg/l.
Amino acid content in the table 1. enayme-decomposed whole egg solution
| Amino acid | Content (mg/ml) | Amino acid | Content (mg/ml) |
| Taurine | ????0.05～0.20 | Methionine | ????0.82～3.58 |
| Aspartic acid | ????1.15～5.55 | Isoleucine | ????0.66～3.96 |
| Threonine | ????0.94～3.94 | Leucine | ????0.49～8.26 |
| Serine | ????1.00～5.35 | Tyrosine | ????0.13～2.43 |
| Glutamic acid | ????1.77～8.9 | Phenylalanine | ????1.23～4.42 |
| Proline | ????0.62～2.44 | Ornithine | ????1.13～3.43 |
| Glycine | ????0.62～3.86 | Lysine | ????1.02～6.47 |
| Alanine | ????0.76～3.69 | Histidine | ????0.42～2.20 |
| Cystine | ????0.65～2.54 | Tryptophan | ????0.11～2.52 |
| Valine | ????1.10～5.94 | Arginine | ????0.67～3.43 |
Enayme-decomposed whole egg solution, is analyzed with the 835-50 of Hitachi type amino-acid analyzer 110 ℃ of hydrolysis 20 hours through 12N HCl again.
Fig. 1 is the analysis collection of illustrative plates of enayme-decomposed whole egg solution at high-performance liquid chromatograph, and chromatographic column adopts G-25 (Sephadex) sephadex; Buffer solution: Tris-Hcl0.1MPH7.4; Flow: 1ml/min, rotating speed: 0.1cm/min; AUFS:1.0.
204,1347,5000 is the standard molecular weight reference among the figure.Abscissa is the time, and ordinate is a labelled amount.
The preparation method of enayme-decomposed whole egg product of the present invention is that the birds, beasts and eggs such as employing egg, duck's egg, goose egg, pigeon eggs, sparrow egg, quail egg are raw material, and the shell egg described in the present invention means egg white whole in the birds, beasts and eggs and yolk. Get yolk and the egg white of birds, beasts and eggs, add the water of 1～4 times of weight, reacted 6～48 hours under 30～70 ℃ with mixed enzyme. Stirring will be conducive to enzymolysis. Described mixed enzyme is two or more mixed enzyme of bromelain, mold protease, bacterialprotease, papain, reagent protease and lipoprotein enzyme. Usually adopt the mixture of reagent protease, bacterialprotease or papain and bromelain, lipoprotein enzyme or two groups of enzymes of mold protease, wherein the two weight ratio of reagent protease, papain or bacterialprotease and bromelain, lipoprotein enzyme or mold protease is 1: 10～1.
Can be in PH5～9 during enzymolysis o'clock carry out. After enzymolysis is finished, adopt the inactivation treatment of heating, usually add thermic and boil and get final product, cooled and filtered, getting clear liquid is enayme-decomposed whole egg solution, also can carry out sterilization treatment in case of necessity. This solution is through concentrated, spray-drying or freeze drying enayme-decomposed whole egg dry powder.
Through animal experiment, being conducive to the weight of animals increases with enayme-decomposed whole egg product of the present invention, well-grown, and without any unusual, maximum tolerated dose is greater than 40ml/Kg. And the proof antagonism width of cloth is penetrated, is improved immunologic function etc. better effects is all arranged; Carry out crowd test through being made into oral liquid, finding has the jaundice of going down to hepatitis patient, falls ascites, falls GPT, increases albumin, reduces globulin, improves a poor appetite, improves the effect of sleep; But in addition to the synthetic of intestines and stomach and operating patient's acceleration bodies internal protein and early rehabilitation; In addition to the anaemia patient, constipation patient, elderly and infirm etc. all have obvious rehabilitation efficacy. The present invention does not just replenish the needs of human nutrition, but various bioactivators in the human body (are activated such as the enzyme source, biological oxidation etc.) play the effect that activates and promote, this also is different from directly replenishing of albumin transfusion, but total essential amino acid of the present invention accounts for the ratio of whole amino acid whose percentages and branched-chain amino acid and aromatic radical acid all is higher than seralbumin and oralbumin, and this is very important progress. So the present invention can be used for various health foods, comprise health drink, health-caring capsule, health instant powder, health care tablet etc.
Adopt method of the present invention, select multiple mixed enzyme that shell egg is carried out enzymolysis, has more narrow spectrum popularity than single enzyme hydrolysis method of planting, protease is cut more thorough, especially make the protein of shell egg resolve into various small-molecular peptides and amino acid, and can preserve the contained and various pure natural vitamins of shell egg and mineral matter etc., have plurality of health care functions, goods have no side effect, and are a kind of easy and lower-cost enzymolysis production process. The enzyme preparation that adopts also all has no side effect, and ADI does not need particular provisions (food additives, revised edition in 1985, light already publishing house). It is a kind of industrial method that is suitable for.
To help to understand the present invention by following embodiment, but be not limited to content of the present invention.
Embodiment 1
Take by weighing the egg white and the protein 10 00g of the shell egg of egg, egg or sparrow egg, add distilled water 1500g, bromelain 2～5g, bacterialprotease 10g, stirring reaction was heated to and boils after 10～15 hours in the time of 20～50 ℃, cooling is filtered, and promptly gets enayme-decomposed whole egg solution 1 behind pressure sterilizing.This solution appearance is a light brown yellow, bitter taste slightly, and recording organic nitrogen content by China's pharmacopeia method is 1～0.6%, pH value is 5.8 ± 0.5.
This enayme-decomposed whole egg solution is surveyed molecular weight distribution with G-25 (Sephadex) column chromatography method, and with the standard molecular weight reference that is respectively 204,1347 and 5000, the result is plotted among Fig. 1.Wherein molecular weight＜1000 account for 70%, molecular weight＞1000 account for 30%, show be one based on the amino acid of small-molecular peptides and the mixture of polypeptide.
Embodiment 2
Change the bromelain of embodiment 1 into the 10g mold protease, all the other conditions are identical, obtain enayme-decomposed whole egg solution.
With the enayme-decomposed whole egg solution of embodiment 1 and 2, after 20 hours, analysis of amino acid is as shown in table 2 through 12NHCl, 110 ℃ of hydrolysis.
| Amino acid | Implement 2 content mg/ml | Implement 1 content mg/ml | Amino acid | Implement 2 content mg/ml | Implement 1 content mg/ml |
| Taurine | ????0.05 | ????0.11 | Methionine | ????0.82 | ????1.58 |
| Aspartic acid | ????2.94 | ????4.69 | Isoleucine | ????1.66 | ????2.76 |
| Threonine | ????1.24 | ????2.10 | Leucine | ????2.49 | ????4.26 |
| Serine | ????1.40 | ????2.35 | Tyrosine | ????0.13 | ????0.43 |
| Glutamic acid | ????3.77 | ????5.91 | Phenylalanine | ????1.23 | ????1.42 |
| Proline | ????0.62 | ????1.14 | Ornithine | ????1.43 | ????2.43 |
| Glycine | ????1.02 | ????1.66 | Lysine | ????2.02 | ????3.47 |
| Alanine | ????1.76 | ????2.81 | Histidine | ????0.62 | ????1.20 |
| Cystine | ????2.05 | ????0.54 | Tryptophan | ????0.31 | ????0.52 |
| Valine | ????3.21 | Arginine | ????0.67 | ????0.43 |
Implementing 1 total amino acid content is 43mg/ml, and implementing 2 total amino acid contents is 26mg/ml, and the enayme-decomposed whole egg freeze-dried powder that makes is a pale yellow powder.
Embodiment 3
With the egg white and the yolk of 1000g shell egg, add 1000g distilled water, reagent protease 5～10g, mold protease 8～12g, after fully stirring, at 40 ℃, PH6～7 o'clock reaction was heated to and boils after 12 hours, and cold filtration is bottled after getting the clear liquid sterilization treatment.Its amino acid percentage component and the white egg of serum and oralbumin amino acid percentage composition (practical biochemistry, the first volume, 1961,81, People's Health Publisher) are if comparative result is as shown in table 3.
With the enayme-decomposed whole egg solution of embodiment 1,2 and 3, carry out calcium, phosphorus, iron, zinc and content of iodine analysis, the result is followed successively by 180～720,160～980,10～100,4～20 and 1～50mg/l respectively.
Table 3 enayme-decomposed whole egg and seralbumin and the percentage composition of oralbumin amino acid are relatively
| Amino acid | Enayme-decomposed whole egg | Seralbumin | Oralbumin |
| Taurine | ????0.26 | ?????～ | ?????～ |
| Aspartic acid | ????10.9 | ????8.5 | ????8.2 |
| Threonine | ????4.9 | ????5.9 | ????3.5 |
| Serine | ????5.5 | ????5.8 | ????7.3 |
| Glutamic acid | ????13.7 | ????14.1 | ????14.6 |
| Proline | ????2.7 | ????4.2 | ????2.9 |
| Glycine | ????3.8 | ????1.7 | ????2.7 |
| Alanine | ????6.5 | ????5.1 | ????5.9 |
| Cystine | ????1.3 | ????5.4 | ????2.5 |
| Valine | ????7.5 | ????5.0 | ????7.8 |
| Methionine | ????3.7 | ????1.1 | ????4.7 |
| Isoleucine | ????6.4 | ????2.5 | ????6.2 |
| Leucine | ????9.9 | ????10.0 | ????8.8 |
| Tyrosine | ????1.0 | ????4.6 | ????3.3 |
| Phenylalanine | ????3.3 | ????5.8 | ????6.8 |
| Ornithine | ????5.6 | ?????～ | ?????～ |
| Lysine | ????8.0 | ????10.5 | ????5.8 |
| Histidine | ????2.8 | ????3.9 | ????2.3 |
| Tryptophan | ????1.2 | ????0.8 | ????1.1 |
| Arginine | ????1.0 | ????4.9 | ????5.2 |
| Amount to | ????99.96％ | ????99.8％ | ????99.6％ |
| Must amino acid | ????44.9％ | ????41.6％ | ????44.6％ |
| Branched-chain amino acid/ArAA | ????5.53 | ????1.68 | ????2.26 |
Embodiment 4
With the enayme-decomposed whole egg solution of embodiment 1,2 and 3, through concentrating back spray-drying or freeze drying, must enayme-decomposed whole egg dry powder, be the pale yellow powder shape, record that wherein to contain organic nitrogen content be 4.5～15%, moisture content is 0～2%.
Claims (7)
1. one kind contains peptide and amino acid whose enayme-decomposed whole egg product, it is characterized in that containing 0.2～15% organic nitrogen, organic matter molecular mass is distributed as 50000～30, wherein molecular weight 〉=1000 accounts for 20～50%, molecular weight＜1000 account for 80～50%, the weight ratio of amino acid and water is 0.014～0.8: 1～0.
2. enayme-decomposed whole egg product as claimed in claim 1; it is characterized in that containing following amino acid: taurine 0.05～0.20mg/ml; aspartic acid 1.15～5.55mg/ml; threonine 0.94～3.94mg/ml; serine 1.00～5.35mg/ml; glutamic acid 1.77～8.9mg/ml; proline 0.62～2.44mg/ml; glycine 0.62～3.86mg/ml; alanine 0.76～3.09mg/ml, cystine 0.65～0.54mg/ml, valine 1.10～5.34mg/ml; methionine 0.82～3.58mg/ml; isoleucine 0.66～3.96mg/ml, leucine 0.49～8.26mg/ml, tyrosine 0.13～2.43mg/ml; phenylalanine 1.23～4.42mg/ml; ornithine 1.13～3.43mg/ml, lysine 1.02～6.47mg/ml, histidine 0.42～2.20mg/ml; tryptophan 0.11～2.52mg/ml, arginine 0.67～3.43mg/ml.
3. an enayme-decomposed whole egg product as claimed in claim 2 is characterized in that containing calcium 180～720mg/l, phosphorus 160～980mg/l, iron 10～100mg/l, zinc 4～20 and iodine 1～50mg/l.
4. the preparation method of an enayme-decomposed whole egg product as claimed in claim 1, it is characterized in that egg white of eggs and albumen are added 1～4 times of weight water, with mixed enzyme 30～70 ℃ and PH5～9 o'clock, stirring 6～48 hours mixed enzyme of enzymolysis is that reagent protease, bacterialprotease or papain and bromelain, lipoprotein enzyme and bacterialprotease, lipoprotein enzyme or the two weight ratio of mold protease are 1: 0.1-1, the required enzyme unit of every gram shell egg is 1000-10000.
5. the preparation method of an enayme-decomposed whole egg product as claimed in claim 4, it is characterized in that solution ebuillition of heated with enzymolysis after, cooling, filter.
6. the preparation method of an enayme-decomposed whole egg product as claimed in claim 5 is characterized in that through freeze drying or spray-drying.
7. the purposes as claim 1,2,3,4,5 or 6 described enayme-decomposed whole egg products is characterized in that being used for health food.
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| CN96116615A CN1154802A (en) | 1996-12-12 | 1996-12-12 | Enayme-decomposed whole egg product, preparation method and use thereof |
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| CN96116615A CN1154802A (en) | 1996-12-12 | 1996-12-12 | Enayme-decomposed whole egg product, preparation method and use thereof |
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| CN1321192C (en) * | 2003-07-18 | 2007-06-13 | 上海复旦新杨生物科技有限责任公司 | Method for preparing egg white peptide by enzyme method hydrolyzing egg white |
| CN102845707A (en) * | 2011-06-30 | 2013-01-02 | 东莞市美拉德食品有限公司 | Production technology for producing nutrient seasoning powder by enzymatic method with comprehensive utilization of avian skeleton |
| CN104187837A (en) * | 2014-07-15 | 2014-12-10 | 东北农业大学 | Instant yolk protein powder containing biologically active substances, and preparation method thereof |
| CN105581274A (en) * | 2015-12-18 | 2016-05-18 | 湖南尔康制药股份有限公司 | Starch capsule filled with freeze-dried egg product |
| CN106333276A (en) * | 2016-08-30 | 2017-01-18 | 滁州学院 | Novel dried egg and processing technology thereof |
| CN107467647A (en) * | 2016-06-07 | 2017-12-15 | 内蒙古伊利实业集团股份有限公司 | It is a kind of that there is the functional composition for improving sleep and its application |
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- 1996-12-12 CN CN96116615A patent/CN1154802A/en active Pending
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| CN1321192C (en) * | 2003-07-18 | 2007-06-13 | 上海复旦新杨生物科技有限责任公司 | Method for preparing egg white peptide by enzyme method hydrolyzing egg white |
| CN102845707A (en) * | 2011-06-30 | 2013-01-02 | 东莞市美拉德食品有限公司 | Production technology for producing nutrient seasoning powder by enzymatic method with comprehensive utilization of avian skeleton |
| CN102845707B (en) * | 2011-06-30 | 2013-10-30 | 东莞市美拉德食品有限公司 | Production technology for producing nutrient seasoning powder by enzymatic method with comprehensive utilization of avian skeleton |
| CN104187837A (en) * | 2014-07-15 | 2014-12-10 | 东北农业大学 | Instant yolk protein powder containing biologically active substances, and preparation method thereof |
| CN105581274A (en) * | 2015-12-18 | 2016-05-18 | 湖南尔康制药股份有限公司 | Starch capsule filled with freeze-dried egg product |
| CN107467647A (en) * | 2016-06-07 | 2017-12-15 | 内蒙古伊利实业集团股份有限公司 | It is a kind of that there is the functional composition for improving sleep and its application |
| CN107467647B (en) * | 2016-06-07 | 2022-07-15 | 内蒙古伊利实业集团股份有限公司 | Functional composition capable of improving sleep and application thereof |
| CN106333276A (en) * | 2016-08-30 | 2017-01-18 | 滁州学院 | Novel dried egg and processing technology thereof |
| CN106333276B (en) * | 2016-08-30 | 2019-07-30 | 滁州学院 | A kind of dried eggs and its processing technology |
| CN109803542A (en) * | 2016-10-20 | 2019-05-24 | 日清食品控股株式会社 | Dried whole-egg and the extruding egg products for using it |
| EP3673749A4 (en) * | 2016-10-20 | 2020-07-01 | Nissin Foods Holdings Co., Ltd. | Dried whole egg and puffed egg product in which same is used |
| CN109803542B (en) * | 2016-10-20 | 2023-06-09 | 日清食品控股株式会社 | Whole egg powder and puffed egg product using same |
| CN109385458A (en) * | 2018-12-18 | 2019-02-26 | 长春大学 | A kind of method that activated protein exempts from digesting protein for raw material preparation |
| CN109601695A (en) * | 2018-12-18 | 2019-04-12 | 长春大学 | A kind of method that activated protein exempts from digesting protein with soybean protein for raw material preparation |
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