CN1071119C - Enzymolytic pearl liquor - Google Patents
Enzymolytic pearl liquor Download PDFInfo
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- CN1071119C CN1071119C CN94112053A CN94112053A CN1071119C CN 1071119 C CN1071119 C CN 1071119C CN 94112053 A CN94112053 A CN 94112053A CN 94112053 A CN94112053 A CN 94112053A CN 1071119 C CN1071119 C CN 1071119C
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- enzymolysis
- margarita
- aminoacid
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- margarita liquid
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Abstract
The present invention relates to an enzymolysis pearl powder product and a preparing method thereof. The enzymolysis of keratin is carried out by papaya protease under a certain condition, the keratin which can not be easily absorbed by a person in pearl powder is changed into 20 kinds of amino acid capable of being easily absorbed by a human body by enzymolysis, and the 20 kinds of amino acid comprise 8 kinds of essential amino acid and 8 kinds of polypeptide substances. The enzymolysis pearl powder product can be used as nutrient solution and the raw materials of cosmetics.
Description
The present invention relates to product and method with zymyhydrolyzed protein matter.
Margarita is a rarity in motherland's medicine treasure-house, and the curative effect to Margarita in " Mingyi Bielu " to " Compendium of Materia Medica " of the Ming Dynasty of Han dynasty all has definite record.People adopt the method for taking Margarita powder for a long time to make skin keep lubricated fine and smooth always for a long time, but because major ingredients calcium carbonate and some other compositions are difficult for being absorbed by the body in the Margarita powder, particularly the shell keratin protein in the Margarita is difficult for decomposing absorbing in vivo, has therefore hindered the positive role of Margarita powder and its specific function greatly.
Continuous development along with science and technology, improving constantly of people's living standard, utilization exploitation to Margarita powder also has new imagination, the Margarita hydrolyzed solution of domestic report, efficient Margarita powder etc., only be that the calcium carbonate of indissoluble is made soluble organic calcium, it is calcium carbonate and part trace element in fact, and the shell keratin protein does not also utilize.And denomination of invention is the carbonate of the patent application of " water soluble full component Margarita powder series of products new preparation process " CN1051394A with edibility carboxylic acid or amino acid replacement Margarita mineral, conchiolin is separated, make several water soluble full component pearl products, but all have certain weak point with acid, the double Hydrolyze method of enzyme then.
Because Margarita powder fails therefore at present also to be had certain shortcoming by the easy composition that absorbs of complete hydrolysis adult physical ability, the shell keratin protein of one of its main component is not also absorbed fully by human body and makes full use of especially.The object of the invention is to find that a kind of natural active matter can make the Margarita powder enzymolysis become aminoacid that human body very easily absorbs and human body beneficial's little peptide molecule, and Margarita powder is utilized fully, gives full play to its specific function.
The present invention handles Margarita powder earlier with lactic acid, make it become the calcium lactate liquid of solubility, its main component is pearl active calcium and trace element, be not the pearl shell keratin protein and do not dissolve part, the pearl shell keratin protein is hydrolyzed with natural plants active substance papain, makes its aminoacid that obtains 20 kinds, the aminoacid of eight kinds of needed by human is wherein arranged, also have peptide material, improved the function of Margarita powder greatly.
The present invention implements by following steps:
After the lactic acid adding distil water diluted abundant mixing, become lactic acid solution, the Margarita powder gradation is joined in the lactic acid solution, the Margarita powder limit adds, and stir on the limit, after question response is complete, centrifugal, the gained supernatant is Margarita powder activated calcium and trace element, and the precipitation part is the pearl shell keratin protein.
Distilled water is added in the pearl shell protein, heat slightly, yet add carase, cysteine, disodium EDTA, carry out enzymolysis to 35-39 ℃.Behind the enzymolysis, centrifugal, the enzymolysis Margarita liquid, record its total nitrogen content and aminoacid ingredient after, be divided in container mesohigh sterilization after, promptly get product enzymolysis Margarita liquid.
Get enzymolysis Margarita liquid 0.2ml and add 0.2ml 6% sulfosalicylic acid, 10 times of clear liquid dilutions are got 0.05ml and are measured its aminoacid and content of peptides, and the aminoacid total content is 17.58mg/ml.Table 1 is enzymolysis Margarita liquid amino acid analysis result.
Table 1 enzymolysis Margarita liquid amino acid analysis result
Each seed amino acid total content is 17.58mg/ml.
| Aminoacid | mg/ml | Aminoacid | mg/ml |
| Tryptophan | 0.05 | Proline | 0.14 |
| Lysine | 0.79 | Glycine | 3.11 |
| Histidine | 0.03 | Alanine | 2.07 |
| Arginine | 1.47 | Cystine | 0.07 |
| Ornithine | 0.26 | Valine | 0.73 |
| Aspartic acid | 0.49 | Methionine | 2.53 |
| Threonine | 0.18 | Isoleucine | 0.07 |
| Serine | 0.94 | Leucine | 1.17 |
| Glutamic acid | 0.28 | Tyrosine | 0.45 |
| Taurine | 0.25 | Phenylalanine | 2.75 |
Contain the 12-13 peptide material in the enzymolysis Margarita liquid, its molecular weight is about 1500, also contains a submember, its molecular weight 〉=5000.Little peptide is all good than simple aminoacid at intravital absorbance of machine and speed with amino acid whose mixture, and their biological value is higher than pure aminoacid.
The enzymolysis Margarita liquid carries out mice lavage acute toxicity LD by the institute for drug control, Shanghai to medicine toxicity test method
50Test, the result is as follows:
Adopt Kunming hybrid white mice, body weight is 20 grams, is divided into female, male two groups, 10 every group, and same dosed administration, laboratory observation seven days.The enzymolysis Margarita liquid is with every 1ml of maximum tolerated dose gastric infusion, irritate the stomach secondary every day, the hair color of irritating back observation white mice in continuous three days is smooth, active state is good, other all belongs to normally, draw acute toxicity mice lavage 1ml/20 gram, the white mice body weight is equivalent to the 50ml/kg liquid measure and is circulated into the animal gastric, does not all occur dead.This product belongs to nontoxic level.
The primary raw material of enzymolysis Margarita liquid is rare Chinese medicine Margarita powder, papain, Margarita powder is that people take for a long time, do not see that any side effect takes place, and papain has been produced as the beer clarification agent as far back as U.S. Hua Lesitan company in 1911, and its ADI need not particular provisions, and the reaction post-heating makes remaining enzyme deactivation remove by centrifugal.Papain is also as medicinal, its LD
50For 12.5g/kg belongs to nontoxic level product, China's national standard is as food additive.
Raw material of the present invention itself all is fool proof, there is not any toxicity, that gets back after enzymolysis processing is aminoacid and little peptide to the human body beneficial, for example: but Aspartic Acid can be treated hepatopathy, threonine and had its iron salt of lipotropy can treat anemia, histidine antiulcer and promote cell regeneration effect, arginine to be used for the treatment of hepatic coma and male infertility, cystine that azoospermia causes can promote hair growth, prevention senilism and Detoxication or the like.Polypeptide has the physiological function of certain human body equally, for example: have the morphine pentapeptide and the tridecanoic peptide P material of analgesic activity, have the δ sleep inducing peptide of sleep facilitation, can promote the physiological function of the oxytocin etc. of uterine contraction and lactogenic function.
The present invention adopts papain that the angle glutelin matter in the Margarita is carried out enzymolysis, and its enzymatic hydrolysis condition is extremely gentle, and the enzymolysis post-processing approach is easy, and the products obtained therefrom component is stable behind the enzymolysis, and the papain low price can reduce production costs.The method that the present invention adopts has overcome existing traditional mode and has taken Margarita powder, is difficult for absorbed shell keratin originally and becomes aminoacid and the polypeptide that is absorbed by the body easily and make, and has improved the physiological function of Margarita powder to human body greatly.
Illustrate in greater detail the present invention with embodiment below, being interpreted as embodiment, in office where face does not limit the scope of the invention.
Embodiment:
Extracting lactic acid 3.2 liters, add distilled water 42 liters, fully become lactic acid solution behind the mixing, 1 kilogram of Margarita powder gradation is added in the lactic acid solution, add while stirring, after question response is finished, centrifugal, get supernatant and insoluble part, supernatant is pearl active calcium and trace element part, and insoluble part is the pearl shell keratin protein, gets 1 kilogram of wet shell keratin protein again, add distilled water 10 liters under 35-38 ℃, add 15 gram papains, 1 gram cysteine hydrochloride, 1 gram disodium EDTA, finish 35-38 ℃ of back after following enzymolysis 3-5 hour, centrifugal, the enzymolysis Margarita liquid, survey its total nitrogen content after, divide to install in the container, the autoclave sterilization sterilization is product.
Get enzymolysis Margarita liquid 0.2ml and add 6% sulfosalicylic acid, 10 times of clear liquid dilutions, it is as follows to carry out the amino acid analysis result:
Each seed amino acid total content is every milliliter and contains 21.09 milligrams.
| Aspartic acid | 0.57mg | Leucine | 1.40mg |
| Threonine | 0.23mg | Tyrosine | 0.54mg |
| Serine | 1.16mg | Phenylalanine | 2.87mg |
| Glutamic acid | 0.31mg | Ornithine | 0.47mg |
| Proline | 0.08mg | Histidine | 0.11mg |
| Glycine | 3.63mg | Lysine | 0.89mg |
| Alanine | 2.51mg | Tryptophan | 0.05mg |
| Valine | 0.55mg | Arginine | 2.03mg |
| Methionine | 2.89mg | Taurine | 0.68mg |
| Isoleucine | 0.08mg | Cystine | 0.04mg |
Claims (4)
1. the preparation method of an enzymolysis Margarita liquid, by:
The A Margarita powder is stirring reaction, centrifugal in rare lactic acid solution, makes pearl active calcium;
The centrifugal insoluble part shell keratin protein of B, distilled water are heated and are added papain, cysteine hydrochloride, ethylenediaminetetraacetic acid two under 35-38 ℃ respectively and receive that salt stirs evenly, enzymolysis;
The sterilization of C enzymolysis Margarita liquid is formed.
2. the enzymolysis Margarita liquid of method preparation according to claim 1 is characterized in that: amino acid whose content is (mg/ml)
Aminoacid The every ml of content Aminoacid The every ml of content
Tryptophan 0.30-0.60 Proline 0.08-0.14
Lysine 0.79-0.90 Glycine 3.10-3.65
Histidine 0.03-0.11 Alanine 2.05-2.50
Arginine 1.45-2.00 Cystine 0.04-0.07
Ornithine 0.25-0.45 Valine 0.55-0.73
Aspartic acid 0.50-0.60 Methionine 2.50-2.90
Threonine 0.18-0.23 Isoleucine 0.07-0.08
Serine 0.90-1.15 Leucine 1.10-1.40
Glutamic acid 0.28-0.31 Tyrosine 0.45-0.55
Taurine 0.25-0.68 Phenylalanine 2.00-2.75
The aminoacid total content is 17.58mg/ml-21.10mg/ml, contain molecular weight 1500 12, tridecanoic peptide and a part amount be 5000 polypeptide.
3. enzymolysis Margarita liquid preparation method according to claim 1 is characterized in that with papain hydrolysis shell keratin protein.
4. enzymolysis Margarita liquid preparation method according to claim 1 is characterized in that hydrolysis temperature is 35-38 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN94112053A CN1071119C (en) | 1994-02-25 | 1994-02-25 | Enzymolytic pearl liquor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN94112053A CN1071119C (en) | 1994-02-25 | 1994-02-25 | Enzymolytic pearl liquor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1107335A CN1107335A (en) | 1995-08-30 |
| CN1071119C true CN1071119C (en) | 2001-09-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN94112053A Expired - Fee Related CN1071119C (en) | 1994-02-25 | 1994-02-25 | Enzymolytic pearl liquor |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104257583A (en) * | 2014-09-19 | 2015-01-07 | 上海荣神生物化学有限公司 | Pearl enzymolysis solution and preparation method thereof |
| CN104905242A (en) * | 2014-03-14 | 2015-09-16 | 浙江欧诗漫生物股份有限公司 | A method for preparing pearl separated pearl soluble edible calcium and pearl compound whitening factor solution |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2742661B1 (en) * | 1995-12-21 | 1998-07-10 | Texinfine Sa | NEW USE OF NACRE-CONTAINING COMPOSITIONS |
| CN1101700C (en) * | 1997-12-05 | 2003-02-19 | 上海众联生化技术开发有限公司 | Polypeptide oral liquor |
| CN1325063C (en) * | 2005-06-15 | 2007-07-11 | 宋廷生 | L-amino acid water-soluble pearl powder and its preparing method |
| CN101100689B (en) * | 2007-08-07 | 2010-06-23 | 海南京润珍珠生物技术股份有限公司 | Method for preparing pearl active peptide |
| JP5826032B2 (en) * | 2009-09-29 | 2015-12-02 | 株式会社 資生堂 | Collagen production promoting composition |
| CN105640868B (en) * | 2016-01-30 | 2018-09-18 | 海南京润珍珠生物技术股份有限公司 | Pearl amino acid is stayed up late jade-like stone profit facial mask and preparation method thereof |
| CN106075385A (en) * | 2016-08-17 | 2016-11-09 | 贵州佰世合意股份有限公司 | A kind of polypeptide oral liquor and preparation method thereof |
| CN110893201A (en) * | 2018-09-13 | 2020-03-20 | 上海雷允上药业有限公司 | Traditional Chinese medicine composition for improving sleep |
| CN110638836A (en) * | 2019-08-30 | 2020-01-03 | 广东海洋大学 | Application of water-soluble pearl powder in promoting wound healing |
| CN110547458A (en) * | 2019-09-16 | 2019-12-10 | 北海黑珍珠海洋生物科技有限公司 | Preparation method of enzymolysis pearl powder |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1051394A (en) * | 1990-12-06 | 1991-05-15 | 王四明 | Water soluble full component pearl powder series product new preparation process |
| CN1063896A (en) * | 1991-02-04 | 1992-08-26 | 李新建 | The method for preparing liquid pearl by the solid pearl |
-
1994
- 1994-02-25 CN CN94112053A patent/CN1071119C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1051394A (en) * | 1990-12-06 | 1991-05-15 | 王四明 | Water soluble full component pearl powder series product new preparation process |
| CN1063896A (en) * | 1991-02-04 | 1992-08-26 | 李新建 | The method for preparing liquid pearl by the solid pearl |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104905242A (en) * | 2014-03-14 | 2015-09-16 | 浙江欧诗漫生物股份有限公司 | A method for preparing pearl separated pearl soluble edible calcium and pearl compound whitening factor solution |
| CN104257583A (en) * | 2014-09-19 | 2015-01-07 | 上海荣神生物化学有限公司 | Pearl enzymolysis solution and preparation method thereof |
| CN104257583B (en) * | 2014-09-19 | 2017-04-12 | 上海荣神生物化学有限公司 | Pearl enzymolysis solution and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1107335A (en) | 1995-08-30 |
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