CN115413612A - Non-irritant gathering and spawning method for holothuria leucospilota - Google Patents
Non-irritant gathering and spawning method for holothuria leucospilota Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 57
- 241001213462 Holothuria leucospilota Species 0.000 title claims abstract description 38
- 239000002085 irritant Substances 0.000 title abstract description 5
- 241000208340 Araliaceae Species 0.000 claims abstract description 120
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims abstract description 120
- 235000003140 Panax quinquefolius Nutrition 0.000 claims abstract description 120
- 235000008434 ginseng Nutrition 0.000 claims abstract description 120
- 238000009395 breeding Methods 0.000 claims abstract description 45
- 230000001488 breeding effect Effects 0.000 claims abstract description 45
- 235000013601 eggs Nutrition 0.000 claims abstract description 36
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 26
- 238000003860 storage Methods 0.000 claims abstract description 4
- 241000251511 Holothuroidea Species 0.000 claims description 85
- 230000000384 rearing effect Effects 0.000 claims description 53
- 239000013535 sea water Substances 0.000 claims description 44
- 230000008569 process Effects 0.000 claims description 22
- 238000012258 culturing Methods 0.000 claims description 21
- 230000001150 spermicidal effect Effects 0.000 claims description 20
- 231100000344 non-irritating Toxicity 0.000 claims description 13
- 230000017448 oviposition Effects 0.000 claims description 9
- 230000002776 aggregation Effects 0.000 claims description 7
- 238000004220 aggregation Methods 0.000 claims description 7
- 238000004519 manufacturing process Methods 0.000 claims description 6
- 238000004140 cleaning Methods 0.000 claims description 5
- 230000008859 change Effects 0.000 claims description 4
- 150000001875 compounds Chemical class 0.000 claims description 4
- 238000009400 out breeding Methods 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 2
- 241000258195 Holothuria Species 0.000 claims 1
- 210000003608 fece Anatomy 0.000 claims 1
- 239000002245 particle Substances 0.000 claims 1
- 230000000638 stimulation Effects 0.000 abstract description 11
- 238000001035 drying Methods 0.000 abstract description 7
- 238000007599 discharging Methods 0.000 abstract description 3
- 231100000021 irritant Toxicity 0.000 abstract description 3
- 238000002347 injection Methods 0.000 abstract description 2
- 239000007924 injection Substances 0.000 abstract description 2
- 238000010438 heat treatment Methods 0.000 abstract 1
- 241000951471 Citrus junos Species 0.000 description 6
- 230000006698 induction Effects 0.000 description 5
- 230000004720 fertilization Effects 0.000 description 3
- 230000006978 adaptation Effects 0.000 description 2
- 238000005273 aeration Methods 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 208000035404 Autolysis Diseases 0.000 description 1
- 206010057248 Cell death Diseases 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000004931 aggregating effect Effects 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000016087 ovulation Effects 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000028043 self proteolysis Effects 0.000 description 1
- 230000000920 spermatogeneic effect Effects 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a non-irritant gathering and spawning method of holothuria leucospilota. The method comprises the following steps: selecting parent ginseng, breeding the parent ginseng in a storage way, breeding the parent ginseng in a temporary breeding barrel in a temporary way, managing the parent ginseng in the temporary breeding way, gathering the parent ginseng to carry out first spermiation and spawning, collecting fertilized eggs for the first time, managing the parent ginseng after the first spawning, gathering the parent ginseng to carry out second spermiation and spawning, collecting the fertilized eggs for the second time, and recovering the parent ginseng. The method does not need stimulation measures such as drying in the shade, flowing water, heating and the like, does not need injection induced spawning, has high spawning female reference example, can effectively control the proportion of the male ginseng and the female ginseng for discharging sperm, and has the characteristics of simple operation, high efficiency, small damage to the parent ginseng and the like.
Description
Technical Field
The invention belongs to the technical field of aquaculture, particularly relates to the cosmetic raw material industry, and particularly relates to a non-irritating grouping spawning method of holothuria leucospilota.
Background
Sea cucumber has good nutritional and medicinal values, is known as ginseng in the sea and is one of the eight delicacies of sea. The history of eating sea cucumbers in China, east Asia and southeast Asia is long, the sea cucumber eating method is also the largest sea cucumber consuming area in the world, and the huge consumption demand directly stimulates the expansion of the wild sea cucumber fishing industry and becomes the main reason for the increasingly deficient sea cucumber resources in the world. Sea cucumbers are also well-known marine clarifiers, and have a very important role in maintaining the health of a marine ecosystem. The tropical sea cucumbers in the south China sea area are various in variety, and due to the influence of artificial fishing and other human economic activities on the marine environment for more than twenty years, wild sea cucumber resources in the south China sea area are seriously degraded, so that the sustainable utilization of the south China sea cucumber resources is influenced, the marine ecological environment is also adversely affected, and therefore resource maintenance and restoration are urgently needed.
Holothuria leucospilota (Holthus leucospilota) is a common holothurian in the south sea area of China, has the advantages of wide temperature application range, low water quality and substrate requirement conditions, transportation resistance, difficult spitting, difficult autolysis and the like, and is particularly suitable for being used as an object for artificial propagation and culture. In recent years, due to the exhaustion of other sea cucumber resources, the holothuria leucospilota is also developed into food materials in large quantity, and the exhaustion of holothuria leucospilota population resources is aggravated. Therefore, the population resources of the holothuria leucospilota are urgently needed to be restored by an artificial ecological breeding mode.
Artificial breeding is an important way for restoring population resources of sea cucumbers, and a method (ZL 201710964693. X) for stimulating spawning induction by long-term transportation of parent sea cucumbers of Holothuria leucospilota is developed in the early stage, wherein the method mainly adopts stimulation of shade drying and temperature change for induced spawning induction. Afterwards, the induced spawning method of drying in the shade and water stimulation (Huang et al, 2018) and the induced spawning method of wall-climbing and continuous flushing stimulation (ZL 20181179112.2) are adopted. In order to further increase the proportion of the egg-laying female ginseng, an induced spawning method of adding an injection induced spawning agent to the stimulation of the dried in the shade and flowing water (ZL 202111171330.3) is developed. Patent ZL201110022885.1 discloses an induced spawning method for tropical sea cucumbers, and the induced spawning method mainly adopts a water jet stimulation and dark induction stimulation. Therefore, the methods all adopt long-time in vitro stimulation to induce the spermiation and the spawning of the tropical sea cucumbers, and the female sea cucumbers are immediately taken out when spawning and placed in spawning buckets to independently spawn. In the long-term practice of induction of spawning of holothuria leucospilota, the method is not always effective, the operation process is complicated, the labor intensity is high, a plurality of spawning facilities are used, and the parent sea cucumber is greatly damaged after the induction of spawning is finished, so that a spawning method which is simpler and more efficient and has less damage to the parent sea cucumber needs to be further explored.
Disclosure of Invention
In the practical process of artificial breeding of holothuria leucospilota for a long time, the key law of holothuria leucospilota breeding is discovered, and on the basis of mastering the laws, the non-irritating grouping spawning method of holothuria leucospilota is invented, and the breeding efficiency of holothuria leucospilota can be improved by the method.
The technical scheme adopted by the invention is as follows:
a nonirritating clustering spawning method of holothuria leucospilota comprises the following steps:
(1) Parent ginseng is cultivated: transporting wild caught parent sea cucumber to a breeding base, and carrying out breeding in a breeding pond for 3-5 days;
(2) Temporary rearing in a temporary rearing barrel: fishing out holothuria leucospilota from the culture pond, quickly washing the body surface with clean seawater, and temporarily culturing in a temporary culture bucket filled with sterilized clean seawater for 2-6 hours;
(3) Temporary breeding and parent ginseng management: after the parent ginseng is temporarily raised, draining the seawater in the temporary raising barrel at 1-3 pm, cleaning the excrement at the bottom of the barrel, adding clean seawater to the original water volume again, inflating normally, continuously patrolling the temporary raising barrel half an hour later, and removing the excrement at the bottom of the barrel in time through siphoning;
(4) Gathering parent ginseng for the first spermiation and spawning: observing whether the sea cucumbers discharge sperms and lay eggs, and when the male ginseng sperm discharge individuals reach a certain number, if more male ginseng sperm discharge is found, immediately taking out the male ginseng in the sperm discharge state, and placing the male ginseng in a sperm production barrel filled with clean seawater; if the female ginseng is found to lay eggs, no intervention is carried out;
(5) Collecting fertilized eggs for the first time: after the male ginseng at the first end is spermidated, the time is calculated, and after 4-8 hours, the parent ginseng breeding process is stopped; the seawater in the temporary rearing barrel is mixed with the fertilized eggs
Pouring the sea cucumber larvae into a sea cucumber larva culturing pool, and entering a normal sea cucumber larva culturing process;
(6) Managing parent sea cucumber after first spawning: after all the fertilized eggs are collected, adding clean seawater into the temporary rearing barrel again until the volume of the original water body is reached, inflating the temporary rearing barrel normally, continuously patrolling the temporary rearing barrel after half an hour, and removing excrement at the bottom of the temporary rearing barrel in time through siphoning;
(7) Gathering parent ginseng for the second spermiation and spawning: observing whether the sea cucumbers discharge sperms and lay eggs, and when the male ginseng sperm discharge individuals reach a certain number, if more male ginseng sperm discharge is found, immediately taking out the male ginseng in the sperm discharge state, and placing the male ginseng in a sperm production barrel filled with clean seawater; if the female ginseng is found to lay eggs, no intervention is carried out;
(8) And (3) collecting fertilized eggs for the second time: after the first male ginseng head discharges the sperms, the time is calculated, and after 4-8 hours, the parent ginseng breeding process is stopped; the seawater in the temporary rearing barrel is mixed with the fertilized eggs
Pouring the sea cucumber larvae into a sea cucumber larva culturing pool, and entering a normal sea cucumber larva culturing process;
(9) Recovering parent ginseng: and after the collection of the fertilized eggs for the second time is finished, the parent ginseng is moved out of the temporary rearing barrel and is placed into the rearing pond for continuous rearing.
Preferably, in the step (1), the adult of the wild harvested holothuria leucospilota reaches more than 200g, and the individual is plump without damage.
Preferably, in the step (1), during the storage period, the sea cucumber pellet compound feed is fed, and water is changed once a day, wherein the water change amount is 20%.
Preferably, in the step (1), the density of the culture is 10-20 heads/m 2 。
Preferably, in the step (2), each temporary rearing bucket is filled with the sterilized clean seawater with the volume of 600-1200 liters.
Preferably, in the step (2), the number of the temporary rearing parent ginseng in each temporary rearing barrel is 100-150.
Preferably, in the step (2), the Vickers tubes and the discharged excrement are removed by siphoning during the temporary rearing, and the frequency of the removal is 1-2 hours.
Preferably, in steps (4) and (7), the certain number of heads is 2 heads.
Preferably, in the steps (4) and (7), the volume of the clean seawater in the sperm producing barrel is 100-200 liters.
The invention has the beneficial effects that:
compared with the prior art, the method has the advantages that the proportion of the spermatogenic male ginseng is greatly reduced because strong stimulation is not needed, the phenomenon of polyspermia fertilization is effectively reduced, and the pollution of excessive sperms to a water body is effectively reduced. The number of the female holothurians capable of laying eggs greatly exceeds the number of the male holothurians capable of discharging sperm in the same barrel, so that the breeding efficiency of the holothuria leucospilota is improved.
Detailed Description
The following examples are further illustrative of the present invention and are not intended to be limiting thereof.
Example 1
And (4) taking 100 heads of the holothurian paternity sea cucumber from the sea cucumber culture pond at 11/8 in 2022, and hastening parturition by adopting a conventional shade-drying running water stimulation method (Huang et al, 2018). The drying time in the shade is 3 hours, and the sea cucumber is drenched by clean seawater every 20 minutes during the drying time, so that the body surface of the sea cucumber is prevented from being dried. After drying in the shade, the sea cucumbers are collected in a silk net with a 100-mesh sieve and stimulated by flowing water for 1 hour. Then put into 4 spawning barrels filled with 150L of seawater, and 25 heads of parent ginseng are put into each barrel. After half an hour, the sea cucumber is found to be discharged with 10 male ginseng and 3 female ginseng in total, but after the female ginseng is removed, the ovulation stops, so that the sperm production ratio of the male ginseng is 10%, and the egg production ratio of the female ginseng is 3%. Therefore, the male sea cucumber of the holothuria leucospilota is sensitive to the stimulation of the dried in the shade and running water, while the female sea cucumber is not sensitive.
Example 2
The method for non-irritating aggregation spawning of holothuria leucospilota comprises the following specific steps:
(1) Selecting parent parameters: in 2022, 8 and 6 days, wild Yuzu sea cucumber parent sea cucumber is caught in Shenzhen great Yawan, the adult is more than 200g, the individual is full and has no damage, and 360 heads of the wild Yuzu sea cucumber parent sea cucumber are caught in total.
(2) And (3) storing and culturing parent ginseng: 8, 8 months and 8 days in 2022, transporting wild harvested holothuria leucospilota to a famous breeding base, and carrying out breeding in a breeding pond with the breeding density of 15 heads/m 2 When parent sea cucumbers are kept, sea cucumber granular compound feed (Qingdao Yu high-yield feed Co., ltd.) is fed, and water is changed once a day by 20 percent. The cultivation time of the parent ginseng is 4 days.
(3) Temporary rearing in a temporary rearing barrel: in 8/12 am in 2022, 240-head Holothuria leucospilota is fished out from the storage pond by using a fishing net, the body surface is quickly washed by clean seawater, and the sea cucumbers are moved into a temporary culture barrel filled with sterilized clean seawater. The temporary rearing barrel is cylindrical, the diameter of the temporary rearing barrel is 1.3 meters, the depth of the temporary rearing barrel is 0.7 meter, the volume of the seawater filled in the temporary rearing barrel is 900 liters, and the color of the temporary rearing barrel is black. The number of the temporary breeding parent ginseng in each temporary breeding barrel is 120, and the temporary breeding time is 4 hours. In the process, the Vickers tube and the discharged excrement are removed in a siphon mode, and the cleaning frequency is once every 1.5 hours.
(4) Temporary breeding and parent ginseng management: after the parent ginseng is temporarily cultured in an gathering way, at 2 pm on 12 pm of 8 months in 2022 years, the seawater in the temporary culture barrel is drained, the excrement at the bottom of the barrel is cleaned, the clean seawater is added again to the original water volume, and the normal aeration is carried out. After half an hour, continuously patrolling the temporary rearing barrel and timely removing the excrement at the bottom of the barrel through siphoning.
(5) Gathering parent ginseng for the first spermiation and spawning: observing whether the sea cucumber is spermicidal and oviposits, and when the spermicidal individuals of the male ginseng reach 2 heads, if more male ginseng are found to be spermicidal, immediately taking out the male ginseng which is spermicidal, and placing the male ginseng in a reserve spermicidal bucket filled with 150 liters of clean seawater. If the female ginseng is found to lay eggs, no intervention is carried out.
(6) Collecting fertilized eggs for the first time: after the male ginseng is discharged from the first head,and (5) starting to calculate time, and stopping the parent-participated breeding process after 5 hours. The seawater in the temporary rearing barrel is mixed with the fertilized eggs
Pouring the sea cucumber larvae into a sea cucumber larva culturing pond, and entering a normal sea cucumber larva culturing process.
(7) Managing parent sea cucumber after first spawning: and after all the fertilized eggs are collected, adding clean seawater into the temporary rearing barrel again to the original water volume, and normally aerating. After half an hour, continuously patrolling the temporary rearing barrel and timely removing the excrement at the bottom of the barrel through siphoning.
(8) Gathering parent ginseng for the second spermiation and spawning: observing whether the sea cucumbers discharge sperms and lay eggs, when the male ginseng sperm discharge individuals reach 2 heads, if more male ginseng sperm discharge is found, immediately taking out the male ginseng which is discharging sperms, and placing the male ginseng in a reserve sperm production barrel filled with 180 liters of clean seawater. If the female ginseng is found to lay eggs, no intervention is carried out.
(9) And (3) collecting fertilized eggs for the second time: after the first male ginseng is spermidated, the time is calculated, and after 6 hours, the process of breeding the parent ginseng is stopped. The seawater in the temporary rearing barrel is mixed with the fertilized eggs
And pouring the sea cucumber larvae into a sea cucumber larva culturing pond, and entering a normal sea cucumber larva culturing process.
(10) Recovering parent ginseng: and after the collection of the fertilized eggs for the second time is finished, the parent ginseng is moved out of the temporary rearing barrel and is placed into the rearing pond for continuous rearing.
In the operation process of the non-irritant aggregation spawning, 8 male ginseng producing sperms are co-found, the proportion of the produced sperms is 3.3%, 16 female ginseng producing eggs are co-found, and the proportion of the spawning eggs is 6.7%. Therefore, by adopting the method, the number of the spermiation male ginseng is effectively reduced, the proportion of the oviposition female ginseng is greatly improved, and the number of the oviposition female ginseng greatly exceeds the number of the spermiation male ginseng in the same barrel, so that the phenomenon of polyspermia fertilization is effectively reduced, the pollution of excessive sperms to a water body is effectively reduced, and the breeding efficiency of the holothuria leucospilota is improved.
Example 3
The method for non-irritating aggregation spawning of holothuria leucospilota comprises the following specific steps:
(1) Selecting parent parameters: in 2022, 8 and 30 days, wild Yuzu sea cucumber parent sea cucumber is caught in Shenzhen great Yawan, the adult is more than 200g, the individual is full and has no damage, and 200 heads of the wild Yuzu sea cucumber parent sea cucumber are caught in total.
(2) And (3) storing and culturing parent ginseng: transporting 120 head of wild harvested holothuria leucospilota parent sea cucumber to the famous breeding base at 2022, 9 months and 3 days, and breeding in a breeding pond with a breeding density of 10 head/m 2 When parent sea cucumbers are bred, sea cucumber granular compound feed (Qingdao Yufeng aquatic feed Co., ltd.) is fed, and water is changed once a day, wherein the water change amount is 20%. The cultivation time of the parent ginseng is 4 days.
(3) Temporarily breeding in a temporary breeding barrel: in 2022, 9 months and 8 am, 120 head holothurian yuzu is fished out from the breeding pool by using a fishing net, the body surface is quickly washed by clean seawater, and the sea cucumber yuzu is moved into a temporary breeding barrel filled with sterilized clean seawater. The temporary rearing barrel is cylindrical, the diameter is 1.3 m, the depth is 0.7 m, the volume of the seawater filled in the temporary rearing barrel is 850 liters, the color of the temporary rearing barrel is black, and the temporary rearing time is 4 hours. In the process, the Vickers tube and the discharged excrement are removed in a siphoning mode, and the cleaning frequency is once every 1.5 hours.
(4) Temporary breeding and parent ginseng management: after the parent ginseng is temporarily cultured in an aggregating way, at 3 pm of 9/8/2022, the seawater in the temporary culture barrel is drained, the excrement at the bottom of the barrel is cleaned, the clean seawater is added again to the original water volume, and the normal aeration is carried out. After half an hour, continuously patrolling the temporary rearing barrel and timely removing the excrement at the bottom of the barrel through siphoning.
(5) Gathering parent ginseng for the first spermiation and spawning: observing whether the sea cucumber is spermicidal and oviposits, and when the spermicidal individuals of the male ginseng reach 2 heads, if more male ginseng are found to be spermicidal, immediately taking out the male ginseng which is spermicidal, and placing the male ginseng in a reserve spermicidal bucket filled with 150 liters of clean seawater. If the female ginseng is found to lay eggs, no intervention is carried out.
(6) Collecting fertilized eggs for the first time: after the first male ginseng is spermidated, the time is calculated, and after 5 hours, the process of breeding the parent ginseng is stopped. The seawater in the temporary rearing barrel is mixed with the fertilized eggs
Pouring the sea cucumber larvae into a sea cucumber larva culturing pond, and entering a normal sea cucumber larva culturing process.
(7) Managing parent sea cucumber after first spawning: and after all the fertilized eggs are collected, adding clean seawater into the temporary rearing barrel again until the volume of the original water body is reached, and normally aerating. After half an hour, continuously patrolling the temporary rearing barrel and timely removing the excrement at the bottom of the barrel through siphoning.
(8) Gathering parent ginseng for the second spermiation and spawning: observing whether the sea cucumber is spermicidal and oviposits, and when the spermicidal individuals of the male ginseng reach 2 heads, if more male ginseng are found to be spermicidal, immediately taking out the male ginseng which is spermicidal, and placing the male ginseng in a reserve spermicidal bucket filled with 180 liters of clean seawater. If the female ginseng is found to lay eggs, no intervention is carried out.
(9) And (3) collecting fertilized eggs for the second time: after the first male ginseng is subjected to sperm elimination, the time is calculated, and after 6 hours, the process of breeding the parent ginseng is stopped. The seawater in the temporary rearing barrel is mixed with the fertilized eggs
Pouring the sea cucumber larvae into a sea cucumber larva culturing pond, and entering a normal sea cucumber larva culturing process.
(10) Parent ginseng recovery: and after the collection of the fertilized eggs for the second time is finished, the parent ginseng is moved out of the temporary rearing barrel and is placed into the rearing pond for continuous rearing.
In the operation process of the non-irritant aggregation spawning, 5 male ginseng producing sperms are co-found, the proportion of the produced sperms is 4.2%, 8 female ginseng producing eggs are co-found, and the proportion of the spawning eggs is 6.7%. Therefore, by adopting the method, the number of the spermiation male ginseng is effectively reduced, the proportion of the oviposition female ginseng is greatly improved, and the number of the oviposition female ginseng greatly exceeds the number of the spermiation male ginseng in the same barrel, so that the phenomenon of polyspermia fertilization is effectively reduced, the pollution of excessive sperms to a water body is effectively reduced, and the breeding efficiency of the holothuria leucospilota is improved.
The above is only a preferred embodiment of the present invention, and it should be noted that the above preferred embodiment should not be considered as limiting the present invention, and the protection scope of the present invention should be subject to the scope defined by the claims. It will be apparent to those skilled in the art that various modifications and adaptations can be made without departing from the spirit and scope of the invention, and these modifications and adaptations should be considered within the scope of the invention.
Claims (9)
1. A nonirritant clustering spawning method of holothuria leucospilota is characterized by comprising the following steps:
(1) And (3) storing and culturing parent ginseng: transporting wild caught parent sea cucumber to a breeding base, and carrying out breeding in a breeding pond for 3-5 days;
(2) Temporary rearing in a temporary rearing barrel: fishing out holothuria leucospilota from the culture pond, quickly washing the body surface with clean seawater, and temporarily culturing in a temporary culture bucket filled with sterilized clean seawater for 2-6 hours;
(3) Temporary breeding and parent ginseng management: draining seawater in the temporary breeding barrel at 1-3 pm after temporary breeding of parent ginseng, cleaning feces at the bottom of the barrel, adding clean seawater to original water volume, inflating normally, and continuously patrolling the temporary breeding barrel and the parent ginseng after half an hour
The excrement at the bottom of the barrel is removed through siphoning;
(4) Gathering parent ginseng for the first spermiation and spawning: observing whether the sea cucumbers discharge sperms and lay eggs, and when the male ginseng sperm discharge individuals reach a certain number, if more male ginseng sperm discharge is found, immediately taking out the male ginseng in the sperm discharge state, and placing the male ginseng in a sperm production barrel filled with clean seawater;
if the female ginseng is found to lay eggs, no intervention is carried out;
(5) Collecting fertilized eggs for the first time: after the male ginseng at the first end is spermidated, the time is calculated, and after 4-8 hours, the parent ginseng breeding process is stopped; the seawater in the temporary rearing barrel is mixed with the fertilized eggs
Pouring the sea cucumber larvae into a sea cucumber larva culturing pond, and entering a normal sea cucumber larva culturing process;
(6) Managing parent sea cucumber after first spawning: after all the fertilized eggs are collected, adding clean seawater into the temporary rearing barrel again to the original water volume, inflating normally, continuously patrolling the temporary rearing barrel half an hour later, and removing excrement at the barrel bottom in time through siphoning;
(7) Gathering parent ginseng for the second spermiation and spawning: observing whether the sea cucumber is spermicidal and oviposits, and when the spermicidal individuals of the male ginseng reach a certain number, if more male ginseng are found to be spermicidal, immediately taking out the male ginseng which is spermicidal, and placing the male ginseng in a spermicidal bucket filled with clean seawater;
if the female ginseng is found to lay eggs, no intervention is carried out;
(8) Collecting fertilized eggs for the second time: after the first male ginseng head discharges the sperms, the time is calculated, and after 4-8 hours, the parent ginseng breeding process is stopped; the seawater in the temporary rearing barrel is mixed with the fertilized eggs
Pouring the sea cucumber larvae into a sea cucumber larva culturing pool, and entering a normal sea cucumber larva culturing process;
(9) Recovering parent ginseng: and after the collection of the fertilized eggs for the second time is finished, the parent ginseng is moved out of the temporary rearing barrel and is placed into the rearing pond for continuous rearing.
2. The non-irritating holothuria leucospilota gathering and spawning method according to claim 1, wherein in the step (1), the adult of the wild-caught holothuria leucospilota reaches more than 200g, and the individual is plump without damage.
3. The non-irritating egg-laying method for holothuria leucospilota gathering and gathering as claimed in claim 1, wherein in step (1), during the period of storage, the holothuria particle compound feed is fed, and water is changed once a day, wherein the water change amount is 20%.
4. The non-irritating egg-laying method of holothuria leucospilota according to claim 1, wherein in step (1), the stocking density is 10-20 heads/m 2 。
5. The non-irritating egg-laying method of holothuria leucospilota according to claim 1, wherein in step (2), said temporary rearing pots each contain sterilized clean seawater in a volume of 600-1200 liters.
6. The non-irritating aggregation spawning method of holothuria leucospilota according to claim 1, wherein in the step (2), the number of the holothuria leucospilota temporarily cultured in each temporary culture barrel is 100-150.
7. The non-irritating aggregation and spawning method of holothuria leucospilota according to claim 1, wherein in the step (2), the Vickers and the discharged excrement are removed by siphoning during the temporary rearing, and the cleaning frequency is once every 1-2 hours.
8. The non-irritating egg-laying method of holothuria leucospilota according to claim 1, wherein said certain number of heads in steps (4) and (7) is 2 heads.
9. The non-irritating aggregation spawning method of holothuria leucospilota according to claim 1, wherein the volume of clean seawater in the spawning tank is 100-200 liters in steps (4) and (7).
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