CN109952981A - A method of improving stichopus japonicus parent egg laying amount - Google Patents
A method of improving stichopus japonicus parent egg laying amount Download PDFInfo
- Publication number
- CN109952981A CN109952981A CN201910382873.6A CN201910382873A CN109952981A CN 109952981 A CN109952981 A CN 109952981A CN 201910382873 A CN201910382873 A CN 201910382873A CN 109952981 A CN109952981 A CN 109952981A
- Authority
- CN
- China
- Prior art keywords
- stichopus japonicus
- parent
- oviposition
- seawater
- added
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000017448 oviposition Effects 0.000 title claims abstract description 52
- 241000965254 Apostichopus japonicus Species 0.000 title claims abstract description 38
- 238000000034 method Methods 0.000 title claims abstract description 28
- 239000013535 sea water Substances 0.000 claims abstract description 26
- 235000013601 eggs Nutrition 0.000 claims abstract description 23
- 238000010438 heat treatment Methods 0.000 claims abstract description 23
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 11
- 239000001301 oxygen Substances 0.000 claims abstract description 11
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 11
- 230000002710 gonadal effect Effects 0.000 claims abstract description 5
- 230000035800 maturation Effects 0.000 claims abstract description 5
- 230000000638 stimulation Effects 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- 238000004519 manufacturing process Methods 0.000 claims description 9
- 210000004392 genitalia Anatomy 0.000 claims description 3
- 210000004907 gland Anatomy 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims description 3
- 238000009360 aquaculture Methods 0.000 abstract description 4
- 244000144974 aquaculture Species 0.000 abstract description 4
- 241000251511 Holothuroidea Species 0.000 abstract description 3
- 102000002322 Egg Proteins Human genes 0.000 description 9
- 108010000912 Egg Proteins Proteins 0.000 description 9
- 210000004681 ovum Anatomy 0.000 description 9
- 241000251468 Actinopterygii Species 0.000 description 3
- 230000012447 hatching Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 244000144987 brood Species 0.000 description 2
- 238000002224 dissection Methods 0.000 description 2
- 241000208340 Araliaceae Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 230000026109 gonad development Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 230000004938 stress stimulation Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/30—Culture of aquatic animals of sponges, sea urchins or sea cucumbers
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The present invention relates to a kind of methods for improving stichopus japonicus parent egg laying amount, belong to aquaculture field, the method is to grab the stichopus japonicus parent of gonadal maturation, the spawning pond not being oxygenated is put into after rinsing well, heating seawater is added portionwise according to stichopus japonicus parent oviposition situation, until all oviposition finishes stichopus japonicus parent.The method of the present invention is added portionwise fresh seawater by control seawater addition opportunity and dissolved oxygen is caused to stimulate, and lures sea cucumber single as far as possible that whole mature eggs are discharged, improves egg laying amount and spawning efficiency, reduces parent participation and cultivates again and the cost of secondary oviposition.
Description
Technical field
The invention belongs to aquaculture fields, more particularly to a kind of method for improving stichopus japonicus parent egg laying amount.
Background technique
Stichopus japonicus has high nutritive value and drug value, since seedling-raising technique the 1980s is broken through, China
Apostichopus japonicus culture is able to tremendous development, and scale is expanded rapidly, and cofferdam, pond culture and shallow seabed sowing aquaculture stichopus japonicus have become heat
Point, economic benefit are huge.Statistical data shows that national apostichopus japonicus culture area breaks through 200,000 hectares, 210,000 tons of cultured output, directly
30,000,000,000 yuan of production value, apostichopus japonicus culture had become the another crucial pillar aquaculture industry after shellfish, algae, fish already.
Parent participation oviposition is the key technique of stichopus japonicus artificial breeding, and the mode of Stichopus Japonicus Selenka to lay eggs mainly includes two kinds at present,
One is natural spawnings, another is artificial stimulation oviposition.Natural spawning is due to cannot artificially control egg-laying time and oviposition
Amount, thus be easy to happen adopt ovum cause not in time ovum amount lose.Later period develops artificial with the progress of artificial breeding technology
Oviposition technology is stimulated, currently used artificial stimulation oviposition method includes heating stimulus method, dark dry technique and stimulation by running water method,
The foundation of the technology can arrange production taking human as parent participation egg-laying time is grasped convenient for planned.But artificial stimulation at present produces
Ovum still faces parent participation and lays eggs halfway problem, dissection discovery artificial stimulation parent participation single egg laying amount only account for fish brood amount 50~
60%, it needs to carry out the parent participation after oviposition cultivation and secondary oviposition again, causes the increase of production cost and labor cost.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of methods for improving stichopus japonicus parent egg laying amount, carry out to parent participation
On the basis of heating stimulation, changes the inflation strategy of parent participation spawning pond and add water strategy, more as far as possible using dissolved oxygen stimulation parent participation
Ovum is discharged, improves the single egg laying amount of parent participation, realizes the efficient utilization of parent participation, improves production efficiency, reduces production cost.
The present invention is realized by following technical solution:
A method of stichopus japonicus parent egg laying amount is improved, the stichopus japonicus parent of gonadal maturation is grabbed, is put after rinsing well
Enter the spawning pond not being oxygenated, heating seawater is added portionwise according to stichopus japonicus parent oviposition situation, until stichopus japonicus parent has all been laid eggs
Finish.
Further, the stichopus japonicus parent of the gonadal maturation is that genital gland indices are greater than 10% or more.
Further, the heating seawater being added, temperature are 21~22 DEG C, and the temperature than stichopus japonicus parent culture water is high by 3~5
DEG C, achieve the purpose that heating stimulation oviposition.
Further, after stichopus japonicus parent is put into spawning pond, heating seawater is added portionwise, i.e., oviposition tank depth one is added for the first time
Half (about 50cm) heating seawater, a large amount of stichopus japonicus parents climb to oviposition pool wall in the case where dissolved oxygen stimulates and heat up stimulation and produce for the first time
Ovum；At the end of first time egg production peak time, the fresh heating seawater of depth of water 10cm is added into spawning pond, due to the sea being newly added
Water dissolved oxygen is higher, and stichopus japonicus parent can be stimulated to continue to climb to second of oviposition of water surface progress；Knot is closed in second of spawning peak
Shu Shi, is added the fresh heating seawater of depth of water 10cm into spawning pond again, and stimulation stichopus japonicus parent third time is laid eggs；And so on,
Stichopus japonicus parent is stimulated to lay eggs by the method that fresh seawater is repeatedly added portionwise, until oviposition all terminates.
Further, it after oviposition, is inflated into spawning pond, ensures fertilized eggs normal incubation.
The present invention compared with prior art the utility model has the advantages that
Artificial stimulation Stichopus Japonicus Selenka to lay eggs technology (heating stimulus method, dark dry technique and stimulation by running water method) single traditional at present
Egg laying amount only accounts for the 50~60% of fish brood amount, and there are parent participation egg laying amount is low, halfway problem of laying eggs is needed to the parent after oviposition
Ginseng carries out cultivation and secondary oviposition again, causes the increase of production cost and labor cost.The present invention is by building low dissolved oxygen
Pool wall discharge ovum (sperm) is climbed in stress, stimulation parent participation as early as possible, in first round oviposition coda, by the way that fresh seawater is added batch-wise
(dissolved oxygen amount is higher) lures parent participation to continue to swash and carry out new round oviposition.In this way, opportunity point is added by control seawater
Batch fresh seawater is added causes dissolved oxygen to stimulate, and lures sea cucumber single as far as possible that whole mature eggs are discharged, improve egg laying amount and
Spawning efficiency reduces parent participation and cultivates again and the cost of secondary oviposition.
Specific embodiment
Describe technology contents of the invention in detail below by embodiment, but protection scope of the present invention is not appointed by embodiment
What formal limitation.
Embodiment 1
In April, 2019, using the parent participation that artificial ecology accelerating is cultivated as parent, parent culture water temperature is 15~17 DEG C, is led to
The mode of oversampling dissection differentiates gonad development of parents situation, when genital gland indices are greater than 10% or more, produces as parent
Ovum.
Grab 500 parents, with clean seawater flushing it is clean after be put into spawning pond, withdraw from the gas stone etc. in spawning pond
Facility is inflated, the heating seawater that temperature is 21~22 DEG C is added into spawning pond, adds the depth of water about 50cm.
Parent participation climbs to the water surface along pool wall and carries out first time oviposition (or sperm drainage) under heating stimulation and dissolved oxygen stress stimulation,
At the end of first time, egg production peak time closed on, fresh 21~22 DEG C of heatings seawater of depth of water 10cm is added into spawning pond, by
It is higher in the seawater dissolved oxygen being newly added, can stimulate parent participation continue on climb to the water surface and carry out second and lay eggs；It lays eggs at second
At the end of peak is closed on, fresh 21~22 DEG C of heatings seawater of depth of water 10cm, stimulation parent participation third time is added into spawning pond again
Oviposition；And so on, stimulate sea cucumber to lay eggs by the method that fresh seawater is repeatedly added portionwise, until oviposition all terminates.
It after oviposition, is inflated into spawning pond, ensures fertilized eggs normal incubation.
Egg laying amount is counted with sampling, 500 stichopus japonicus parent's single oviposition total amounts are 2,100,000,000, and in parallel only
With heating stimulation and normally in the spawning pond that is oxygenated, 50 stichopus japonicus parent's singles oviposition total amounts are 1,600,000,000, and egg laying amount improves
31.25%.Be 95.3% using the hatching rate of fertilized eggs that the present invention obtains, it is parallel only with heating stimulation be normally oxygenated
The hatching rate of fertilized eggs is 95.1% in spawning pond, it was demonstrated that the sperm or ovum of the method for the present invention stimulation output will not influence fertilization
The hatching rate of ovum.
Claims (5)
1. a kind of method for improving stichopus japonicus parent egg laying amount, it is characterised in that the method is to grab the thorn of gonadal maturation
Join parent, the spawning pond not being oxygenated is put into after rinsing well, heating seawater is added portionwise according to stichopus japonicus parent oviposition situation, until
All oviposition finishes stichopus japonicus parent.
2. according to the method described in claim 1, it is characterized in that the stichopus japonicus parent of the gonadal maturation is genital gland indices
Greater than 10% or more.
3. according to the method described in claim 1, it is characterized in that the heating seawater being added, temperature is 21~22 DEG C, than thorn
The temperature for joining parent culture water is 3~5 DEG C high.
4. according to the method described in claim 1, it is characterized in that heating sea is added portionwise after stichopus japonicus parent is put into spawning pond
Water, i.e., be added the heating seawater of oviposition tank depth half for the first time, and a large amount of stichopus japonicus parents climb in the case where dissolved oxygen stimulates and heat up stimulation
It lays eggs for the first time to oviposition pool wall；At the end of first time egg production peak time, the fresh heating of depth of water 10cm is added into spawning pond
Seawater can stimulate stichopus japonicus parent to continue to climb to second of oviposition of water surface progress since the seawater dissolved oxygen being newly added is higher；?
At the end of secondary spawning peak closes on, the fresh heating seawater of depth of water 10cm, stimulation stichopus japonicus parent the is added into spawning pond again
It lays eggs three times；And so on, stimulate stichopus japonicus parent to lay eggs by the method that fresh seawater is repeatedly added portionwise, until oviposition is all
Terminate.
5. according to the method described in claim 1, it is characterized in that being inflated after oviposition into spawning pond, guarantee fertilized eggs
Normal incubation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910382873.6A CN109952981B (en) | 2019-05-09 | 2019-05-09 | Method for improving egg laying amount of stichopus japonicus parent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910382873.6A CN109952981B (en) | 2019-05-09 | 2019-05-09 | Method for improving egg laying amount of stichopus japonicus parent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109952981A true CN109952981A (en) | 2019-07-02 |
| CN109952981B CN109952981B (en) | 2020-08-28 |
Family
ID=67027094
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910382873.6A Active CN109952981B (en) | 2019-05-09 | 2019-05-09 | Method for improving egg laying amount of stichopus japonicus parent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109952981B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112655637A (en) * | 2020-12-18 | 2021-04-16 | 广东省农业科学院动物科学研究所 | Method for improving tropical sea cucumber culture pond and utilization rate of parent sea cucumber through multiple induced spawning and multiple attachment |
| CN115413612A (en) * | 2022-09-15 | 2022-12-02 | 中国科学院南海海洋研究所 | Non-irritant gathering and spawning method for holothuria leucospilota |
| CN116019036A (en) * | 2022-10-13 | 2023-04-28 | 广东省农业科学院动物科学研究所 | Method for stimulating tropical sea cucumber to spawn and discharge sperms through continuous aeration of water body |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101897305A (en) * | 2010-07-09 | 2010-12-01 | 山东海益宝水产股份有限公司 | Method of artificial feeding of red stichopus japonicus |
| CN102100206A (en) * | 2011-01-04 | 2011-06-22 | 陈水春 | Artificial breeding method for tropical sea cucumbers |
| KR101544706B1 (en) * | 2014-09-01 | 2015-08-18 | 임용택 | Temperature adjustable sea cucumber cultivation system on land |
| CN109220913A (en) * | 2018-10-10 | 2019-01-18 | 中国科学院南海海洋研究所 | A method of hojothuria leucospilota spawning efficiency is improved by lasting stimulation |
-
2019
- 2019-05-09 CN CN201910382873.6A patent/CN109952981B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101897305A (en) * | 2010-07-09 | 2010-12-01 | 山东海益宝水产股份有限公司 | Method of artificial feeding of red stichopus japonicus |
| CN102100206A (en) * | 2011-01-04 | 2011-06-22 | 陈水春 | Artificial breeding method for tropical sea cucumbers |
| KR101544706B1 (en) * | 2014-09-01 | 2015-08-18 | 임용택 | Temperature adjustable sea cucumber cultivation system on land |
| CN109220913A (en) * | 2018-10-10 | 2019-01-18 | 中国科学院南海海洋研究所 | A method of hojothuria leucospilota spawning efficiency is improved by lasting stimulation |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112655637A (en) * | 2020-12-18 | 2021-04-16 | 广东省农业科学院动物科学研究所 | Method for improving tropical sea cucumber culture pond and utilization rate of parent sea cucumber through multiple induced spawning and multiple attachment |
| CN115413612A (en) * | 2022-09-15 | 2022-12-02 | 中国科学院南海海洋研究所 | Non-irritant gathering and spawning method for holothuria leucospilota |
| CN116019036A (en) * | 2022-10-13 | 2023-04-28 | 广东省农业科学院动物科学研究所 | Method for stimulating tropical sea cucumber to spawn and discharge sperms through continuous aeration of water body |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109952981B (en) | 2020-08-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104273091B (en) | A kind of giant salamander Ecology propagation method | |
| WO2017107032A1 (en) | Simulated ecological breeding method for odontobutis obscura | |
| CN102124974B (en) | Method for artificially breeding seed of spotted maigre | |
| CN104273074B (en) | A kind of method for cultivating cuttlefish seedling | |
| CN109952981A (en) | A method of improving stichopus japonicus parent egg laying amount | |
| CN1846480A (en) | Artificial breeding method and incubator for shatang carp | |
| CN104041449B (en) | The Sungkiang weever of simulate natural ecological environment breeds cultivating workshop system | |
| CN105052782B (en) | The artificial fecundation method of Xichang Triplophysa | |
| CN104221958B (en) | A kind of C. guichenoti induced spawning method | |
| CN103125416A (en) | Ecological factorial breeding method of coilia ectenes parent fish | |
| CN101606502A (en) | A kind of method of breeding low temperature-resistant large yellow croakers | |
| CN103548720B (en) | A kind of method of Trachyostracous mussel artificial induced spawning and insemination | |
| CN101112188B (en) | Oogenesis technique for artificial culture of mud crab parent | |
| CN105409755A (en) | Trans-regional relay seedling method for porphyra haitanensis | |
| CN107549064A (en) | Cement pit artificial fecundation method in a kind of Scatophagus argus (Linnaeus) room | |
| CN108235965A (en) | It controllably accelerates the ripening in a kind of egg-shaped pompano parent population room oviposition method | |
| CN103493767B (en) | Odontobulis mpotamophila and Odontobutis yaluensis cross breeding method | |
| CN106942105A (en) | A kind of method for carrying out Hong Kong oyster artificial breeding using the northern megathermal period | |
| CN102524134B (en) | Artificial spawning and insemination method for hyriopsis cumingii | |
| CN105210949A (en) | A kind of method that loach fry maintenance is cultivated | |
| CN101664005A (en) | Industrial and artificial breeding method of Sarotherodon sp | |
| CN102599094B (en) | Method for artificial incubation of Percocypris pingi pingi | |
| CN106359222A (en) | Method for cultivating eriocheir sinensis in small pool | |
| CN102007875A (en) | Leiocassis longirostris intensive artificial propagation method | |
| CN101965816B (en) | Burbot full-artificial ecological control natural breeding technology |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |