CN115317401B - Skin care composition and preparation method and application thereof - Google Patents
Skin care composition and preparation method and application thereof Download PDFInfo
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- CN115317401B CN115317401B CN202210584686.8A CN202210584686A CN115317401B CN 115317401 B CN115317401 B CN 115317401B CN 202210584686 A CN202210584686 A CN 202210584686A CN 115317401 B CN115317401 B CN 115317401B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups
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Abstract
The present invention relates to a composition for protecting exosome activity, said composition comprising a polysaccharide-polypeptide complex; also relates to a skin care composition stable at normal temperature, a preparation method and application thereof. The composition provided by the invention can preserve exosomes at normal temperature and maintain high activity of exosomes. The composition provided by the invention has good cell repair promoting effect, relieving effect, anti-wrinkle tightening effect and capability of remarkably solving various skin problems.
Description
Technical Field
The invention belongs to the field of biology, and in particular relates to a composition for protecting exosome activity; also relates to a skin care composition stable at normal temperature, a preparation method and application thereof.
Background
Skin problems are classified into various kinds, such as skin peeling, color spots, eye bags, dark circles, acne, blackheads, allergies, wrinkles, and the like, which are skin problems. If the skin is desired to be treated with daily adherence, from the viewpoints of cleansing, skin care and the like, the skin is better by taking care of diet and rest, so that various functional products such as functional raw materials of vitamins, peptides, A alcohols and the like are present on the market, the functional raw materials can only stay on the outermost layer of the skin and play a very small role, so that a functional raw material capable of playing a role at all is needed, all problems of the skin can be attributed to the occurrence of problems of cells, and abnormal melanocytes in the skin can occur such as: skin dullness, accumulation of pigments; the problems of wrinkles and the like can occur when fibroblasts in the skin are abnormal, and the skin problems of damaged skin barrier and the like can occur when the horny layer is abnormal, so the problem of the skin is solved, and the problem of the cells is solved.
Exosomes (exosomes) are cup-shaped vesicles of bilayer membrane structure with a diameter of about 30-100nm that can be secreted by a variety of cells. Exosomes can transfer proteins, mRNA, microRNA and other active substances among cells, participate in a plurality of important physiological and pathological processes, and the unique effects of the exosomes are increasingly concerned. Cells secrete exosomes under normal physiological conditions, and exosome-like vesicles are secreted into the extracellular space by exocytosis as a result of fusion between the plasma membrane and the multivesicular body. Exosome-like vesicles are divided into an interior and an exterior by lipid bilayers, which indirectly reflect the nature and status of the cell as they contain membrane lipids, membrane proteins, genetic material and cytoplasmic components. Meanwhile, exosome-like vesicles act as extracellular transport proteins that mediate cell-cell communication by binding to other cells and tissues and transferring membrane components, mRNA, miRNA, proteins (growth hormone, cytokines, etc.), etc. to recipient cells. Current studies indicate that exosomes can exert corresponding skin care effects by repairing damaged cells.
Skin care products with exosomes added already exist on the market at present, but the difficulty of exosomes addition is in preserving activity. It is known to those skilled in the art that exosomes are difficult to ensure their activity at normal temperature. Therefore, there is a need in the art to develop a functional skin care composition that can maintain exosome activity at normal temperature and fully exert its efficacy.
Disclosure of Invention
Aiming at the defects and problems in the prior art, the invention provides a composition for protecting the activity of exosomes and a preparation method thereof, solves the problem of normal-temperature preservation of exosomes in the prior art, and has the advantages of simple production process, strong operability and easy realization.
In a first aspect the invention provides a composition for protecting exosome activity, characterised in that the composition comprises a polysaccharide-polypeptide complex.
Optionally, the exosomes are mesenchymal stem cell exosomes; preferably, the mesenchymal stem cell exosomes are selected from bone marrow mesenchymal stem cell exosomes, umbilical cord blood mesenchymal stem cell exosomes, umbilical cord tissue mesenchymal stem cell exosomes, placenta tissue mesenchymal stem cell exosomes or adipose tissue mesenchymal stem cell exosomes.
Optionally, the polysaccharide-polypeptide complex is a modified polysaccharide modified by a polypeptide or a modified polysaccharide derivative modified by a polypeptide; preferably, the polysaccharide or polysaccharide derivative is at least one of hyaluronic acid or a salt thereof, alginic acid or a salt thereof, hydroxymethyl cellulose or a salt thereof, hydroxypropyl cellulose or a salt thereof, methyl cellulose or a salt thereof, and chitosan or a salt thereof; preferably, the polypeptide is an RGD-containing 3-10 peptide; more preferably, the polypeptide is an RGD-containing octapeptide.
Optionally, the polysaccharide is hyaluronic acid or a sodium salt or potassium salt thereof, preferably the sequence of the polypeptide is Gly-Gly-Gly-Gly-Arg-Gly-Asp-Tyr.
The bioactive molecule RGD peptide (arginine-glycine-aspartic acid) is the polypeptide which has the most wide application and is most effective in promoting cell adhesion at present as an important component of natural ECM. The RGD-containing sequence is used for modifying the surface of the polysaccharide material, so that a cell recognition site can be introduced to simulate the extracellular matrix environment of organisms, thereby better improving the cell affinity of the material. The polysaccharide-polypeptide complex used in the invention is named as HA-RGD (hydrolyzed sodium hyaluronate-Gly-Gly-Gly-Gly-Arg-Gly-Asp-Tyr). The inventors of the present invention have unexpectedly found that such RGD peptide modified polysaccharide material also has the function of protecting exosome activity at normal temperature, which performs cell repair function in a synergistic manner with exosome composition, and verified the remarkable technical advantages of such novel and effective composition in skin care product application through a plurality of effect verification experiments.
Optionally, the modified polysaccharide has a modification percentage of 35% -90%; preferably, the modification percentage is 55% -90%.
Optionally, the composition further comprises a humectant, a preservative, and water; preferably, the humectant is selected from one or more of propylene glycol, butylene glycol, 1, 2-hexanediol, pentylene glycol, β -glucan, glycerol polyether, glycerol, hyaluronic acid or derivatives thereof; the preservative is one or more selected from phenoxyethanol, p-hydroxyphenylethanone and octanediol.
In another aspect, the present invention also provides a skin care composition characterized in that the composition comprises the above polysaccharide-polypeptide complex, exosomes, moisturizers, preservatives and water; preferably, the exosomes are mesenchymal stem cell exosomes; preferably, the humectant is selected from one or more of propylene glycol, butylene glycol, 1, 2-hexanediol, pentylene glycol, β -glucan, glycerol polyether, glycerol, hyaluronic acid or derivatives thereof; the preservative is one or more selected from phenoxyethanol, p-hydroxyacetophenone and octanediol.
Optionally, the composition is prepared from the following components in parts by weight:
humectant: 1.0-10.0 parts; polysaccharide-polypeptide complex: 0.001-0.5 part; exosomes: 10-50.0 parts; preservative: 0.1-0.3 part; 80-120 parts of purified water;
preferably, the composition is prepared from the following components in parts by weight:
humectant: 1.5-5.0 parts; polysaccharide-polypeptide complex: 0.005-0.1 part; exosomes: 10-35.0 parts; preservative: 0.1-0.3 part; 90-100 parts of purified water.
In another aspect, the invention also provides a preparation method of the composition, which is characterized by comprising the following steps:
step 1, respectively weighing the following raw materials in parts by weight:
humectant: 1.0-10.0 parts; polysaccharide-polypeptide complex: 0.001-0.5 part; exosomes: 10-50.0 parts; preservative: 0.1-0.3 part; 80-120 parts of purified water;
step 2, adding the purified water and the humectant weighed in the step 1 into a reaction vessel, mixing, heating to 75-95 ℃, and uniformly stirring to obtain a phase A;
and 3, cooling the phase A to 30-35 ℃, adding the polysaccharide-polypeptide complex, exosomes and preservatives weighed in the step 1 into the phase A obtained in the step 2, and stirring for 20-40 minutes to obtain the polysaccharide-polypeptide complex.
In another aspect, the present invention also provides the use of the skin care composition of the present invention in skin care products or cosmetics. The skin care product or cosmetic is a product commonly used in the field, and comprises but is not limited to freeze-dried powder, a facial mask, skin softening water, essence, emulsion, facial cream, facial cleanser and bath lotion.
The beneficial effects are that:
the skin care composition provided by the invention can preserve exosomes and maintain the high activity of exosomes at normal temperature. Proved by experimental verification, the composition provided by the invention has good cell repair promoting effect, relieving effect, anti-wrinkle tightening effect and capability of remarkably solving various skin problems. The preparation process is simple, has low cost and is suitable for large-scale industrial production. The invention has wide application prospect in the field of cosmetics.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below.
FIG. 1 is a graph showing the comparison of protein content before and after 72 hours for compositions 1-5 of the present invention.
FIG. 2 is a graph showing the cell proliferation potency data of compositions 1-5 of the present invention.
FIG. 3 is a graph of cell migration capacity data for compositions 1-5 of the present invention.
FIGS. 4 and 5 are graphs of the data from the soothing effect test of compositions 1-5 of the present invention.
FIG. 6 is a graph of anti-wrinkle tightening test data for compositions 1-5 of the present invention.
Detailed Description
The invention will be further illustrated with reference to the following examples, which are to be understood as merely further illustrating and explaining the invention and are not to be construed as limiting the invention.
Unless defined otherwise, technical and scientific terms used in this specification have the same meaning as commonly understood by one of ordinary skill in the art. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, the materials and methods are described herein below. In case of conflict, the present specification, including definitions, will control. In addition, the materials, methods, and examples are illustrative only and not intended to be limiting.
Example 1
The embodiment provides a preparation method of the skin care composition, which comprises the following raw materials of purified water, glycerol, propylene glycol, exosomes, HA-RGD (hydrolyzed sodium hyaluronate-Gly-Gly-Gly-Arg-Gly-Asp-Tyr), hydrolyzed sodium hyaluronate and phenoxyethanol.
HA-RGD: available from Shaanxi future polypeptide biotechnology Co., ltd., CAS number: 120022-92-6, lot number: 220401. the modification percentage is 55-90%.
Exosomes: the umbilical cord extract was purchased from guangdong fu Jin Gan cell regeneration medicine limited, lot number: 220203.
hydrolysis of sodium hyaluronate: purchased from Shandong An Hua, lot 211001.
Phenoxyethanol: purchased from sumei, germany, lot number: 210501.
the glycerol and propylene glycol are all analytically pure.
Preparation of compositions 1-5:
step 1, respectively weighing raw materials (unit: g) according to the following table:
step 2, adding the purified water, the glycerol and the propylene glycol weighed in the step 1 into a pot, mixing, heating to 85 ℃, and uniformly stirring to obtain a phase A;
and 3, cooling to 30-35 ℃, adding the HA-RGD, exosomes and phenoxyethanol weighed in the step 1 into the phase A obtained in the step 2, and stirring for 30min to obtain the composition 1-5.
Example 2
Protein content testing:
the compositions 1 to 5 prepared in example 1 were left at room temperature for 72 hours, and the protein content of the compositions 1 to 5 was measured. The method comprises the following steps: and detecting the concentration of BCA protein, wherein the adopted kit is the Sieimer purchase. If the protein content is basically consistent with the protein content in preparation, the protein content loss rate is the smallest, and the activity preservation degree of exosomes is proved to be good; if the protein content loss rate is maximum, the exosome activity is not well preserved, so that the corresponding efficacy is greatly reduced. The experimental results are shown in FIG. 1.
As can be seen from fig. 1, in the compositions 1 to 5, the protein content loss rate in the composition 2 was highest after 72 hours, because there was no protectant, and the protein content loss rates were in this order: composition 3> composition 4> composition 5, which shows that after increasing HA-RGD, the protein content loss rate is reduced, which shows that the substance HAs the ability to protect exosome activity, and as HA-RGD increases, the protein content loss rate of composition 3 and composition 4 is significantly reduced, which shows that as HA-RGD increases in exosome compositions, the protein content loss rate is significantly reduced, which shows that HA-RGD can retain more exosome proteins, and HAs the function of protecting exosome integrity and activity.
Example 3
Cell proliferation potency test:
the growth and development of skin, metabolic cycle and skin cell activity have a certain correlation, and the purpose of stabilizing skin barrier and promoting skin renewal can be achieved by promoting the activity of keratinocytes. The cell activity of the fibroblast refers to the proportion of healthy cells in a sample population, and can strengthen the cell activity of keratinocytes, thereby achieving the purposes of stabilizing skin barrier and promoting skin renewal. Therefore, the cell proliferation capacity can be characterized by the cell activity of the fibroblast, and the strong cell proliferation capacity can indicate that the cell repair effect is very good.
The experimental groupings were as follows: using a detection model of Shaanxi Boxi general detection technology Co., ltd: the fibroblast model is detected:
the test results are shown in fig. 2.
As can be seen from the above data, in compositions 1-5, the fibroblast viability was in turn: composition 1<2<3<4<5, composition 2>1 demonstrates that composition 2 exosomes have an effect of increasing fibroblast viability; the composition 3 added with HA-RGD HAs stronger fibroblast activity than the composition 2 without HA-RGD, which shows that the composition of HA-RGD and exosomes HAs stronger effect of promoting cell proliferation. As the HA-RGD content increased, the fibroblast activity of composition 3-composition 4 increased significantly, indicating that as HA-RGD increased, the exosome activity increased, indicating that the amount of HA-RGD affected the exosome activity. Composition 5> composition 4, it is shown that with the increase of the exosome content, the cell activity is significantly increased, and the exosome content is as high as 25% in consideration of the cost factor when the exosome content is in close proximity to the positive value in composition 5, so that the effect of cell proliferation can be satisfied, the effect of cell repair is caused, and the cost is saved. The experiment proves that the composition has obvious cell repair promoting function.
Example 4
Cell migration ability test:
cell migration refers to movement of cells upon receiving a migration signal or upon sensing the stimulation of certain substances. The increase in cell migration capacity indicates an effect of promoting wound repair. After the skin is damaged, various cells in the skin are mainly restored and activated through cell migration to the damaged part, and the migration capacity of keratinocytes plays an important role in repairing the skin damage.
The experimental groupings were as follows: using a detection model of Shaanxi Boxi general detection technology Co., ltd: the fibroblast model is detected:
the test results are shown in FIG. 3.
As can be seen from the above data, in compositions 1-5, fibroblast migration was in turn: composition 1<2<3<4<5, composition 2>1 demonstrates that composition 2 exosomes have an effect of increasing fibroblast migration; composition 3 with added HA-RGD had stronger fibroblast migration than composition 2 without added HA-RGD, indicating that the combination of HA-RGD and exosomes had stronger cell repair promoting effects. As the HA-RGD content increased, the fibroblast migration of composition 3-composition 4 increased significantly, indicating that as HA-RGD increased, the exosome activity increased, indicating that the amount of HA-RGD affected the exosome activity. Composition 5> composition 4, it is shown that with increasing exosome content, cell migration increases significantly, and when composition 5 is in close proximity to positive, the exosome addition is up to 25% in consideration of cost factors, which not only can satisfy the effect of promoting cell repair, but also saves cost. The experiment proves that the composition has obvious cell repair promoting function.
Example 5
Relief test:
lipopolysaccharide LPS is a component of the outer wall of the cell wall of gram-negative bacteria, is a substance (glycolipid) composed of lipid and polysaccharide, and the pathogenic mechanism of pathogenic bacteria is mainly that LPS is combined with Toll-like receptors to activate downstream inflammatory signal paths, so that inflammatory reactions are activated, and erythema, pain and other clinical phenomena are generated by the inflammatory reactions. In the experiment, macrophages are used as a research model, an in-vitro inflammation model is established by adopting LPS stimulation, and the relief effect of an analyte is analyzed by detecting an inflammation related index (TNF alpha/IL-1 alpha). TNFa/IL 6 is two pro-inflammatory factors, and is mainly synthesized by the typical signal path NF- κB signal path to induce inflammatory cascade reaction, and the content of the TNFa/IL 6 is reduced, so that the sample has a relieving effect. Interleukin-1 alpha (IL-1 alpha) is also known as a source of leukocyte heat, endogenous mediators of leukocytes, monocyte factors, lymphocyte activating factors, IL-1 alpha is mature mediated by the inflammatory body, and is secreted outside the cell after maturation to further bind to the receptor to mediate the cascade amplification of inflammatory responses. The content of the sample is reduced after the sample is acted, which shows that the sample has a relieving effect.
The experimental groupings were as follows: using a detection model of Shaanxi Boxi general detection technology Co., ltd:and (3) detecting:
the detection results are shown in fig. 4 and 5.
The above data shows that the compositions 1-5 contain TNFα and IL-1α in the following order: composition 1>2>3>4>5, composition 2< 1 illustrates that composition 2 exosomes have a reducing effect on both TNFα and IL-1α, and have a soothing effect; the soothing effect of the composition 3 with added HA-RGD is stronger than that of the composition 2 without added HA-RGD, which shows that the composition of HA-RGD and exosomes HAs stronger soothing effect. The soothing effect of composition 3-composition 4 increased significantly with increasing HA-RGD content, indicating that the exosome activity increased with increasing HA-RGD, indicating that the amount of HA-RGD affects exosome activity. Composition 5 < composition 4, it shows that as the content of exosomes increases, the soothing effect increases, and when composition 5 is very close to positive, the exosomes are added up to 25% in maximum in consideration of cost factors, so that the soothing effect can be met, and the cost is saved. This experiment demonstrates that the composition of the present invention has a significant soothing function.
Example 6
Anti-wrinkle tightening test:
in the test, fibroblasts are taken as a research object, an in-vitro photo-aging model is established by adopting UVA irradiation, and the tightening and anti-wrinkle efficacy of an object to be tested is analyzed through the change of the content of Collagen I after the object to be tested is treated. The Collagen I is Collagen I, and the Collagen accounts for about 80% of the dermis layer of the skin, so that the skin is full and full, the increase of the Collagen I content is promoted, and a certain effect of resisting the generation of wrinkles can be achieved.
The experimental groupings were as follows: using a detection model of Shaanxi Boxi general detection technology Co., ltd: the fibribelast is detected:
the detection results are shown in FIG. 6.
As can be seen from the above data, in compositions 1-5, the Collagen I content was, in order: composition 1<2<3<4<5, composition 2>1 demonstrates that composition 2 exosomes have an increasing effect on Collagen I, have an anti-wrinkle tightening effect; the composition 3 added with HA-RGD HAs stronger anti-wrinkle tightening effect than the composition 2 without HA-RGD, which indicates that the composition of HA-RGD and exosomes HAs stronger anti-wrinkle tightening effect. As the HA-RGD content increases, the anti-wrinkle tightening effect of composition 3-composition 4 increases significantly, indicating that as HA-RGD increases, the exosome activity increases, indicating that the amount of HA-RGD affects exosome activity. Composition 5> composition 4, it is shown that the anti-wrinkle tightening effect is remarkably increased with the increase of the content of exosomes, and the dosage of exosomes is maximally 25% considering the cost factor when the composition 5 is very close to the positive, so that the anti-wrinkle tightening effect is satisfied, and the cost is saved. The experiment proves that the composition has obvious anti-wrinkle tightening function.
Example 7
Human body efficacy test:
75 volunteers were summoned, aged between 28-55 years, and 15 persons in one group, were tested for safety and efficacy using compositions 1-5, respectively. The safety is mainly finished through human body issuing test, and if the volunteer is tried out, no bad feedback exists, the safety is passed; the effectiveness passes the instrument test, and the main indexes mainly comprise: moisture content, elasticity, wrinkles, melanin content, red pigment content, TEWL (percutaneous moisture loss). The German CK instrument is used for testing, long-acting effect verification is carried out, the effect data after 4 weeks are used for average value processing, and the improvement degree after nursing is calculated, so that the ratio of the number of people to be improved and the ratio of the number of people not to be improved are obtained. The results were as follows: the safety test has no bad feedback ratio of >90%, and is safe. The specific index judgment basis of efficacy verification is as follows: the numerical value of the instrument after use is more than 3 before use, the instrument is effective, and the proportion of the number of effective people is more than 50 percent.
From the above results, it can be seen that long-term tests for four weeks show that the composition 3 HAs long-term effects of anti-wrinkle tightening, skin brightening and skin barrier repairing by detecting the indexes of moisture content, elasticity, wrinkles, melanin content, red pigment content and TEWL of the subjects after using the composition 3 and the composition 2, and the composition 3 HAs more remarkable effects of anti-wrinkle tightening, skin brightening and skin barrier repairing after adding HA-RGD, so that the composition HAs very remarkable improvement on skin problem solving; with increasing HA-RGD content, the ability of composition 3 and composition 4 to solve skin problems increased, indicating that with increasing HA-RGD in the composition, the ability to solve skin problems increased, indicating that HA-RGD HAs the activity of protecting exosomes, making the ability to solve skin problems stronger. Composition 5> composition 4 has significantly increased ability to solve skin problems with increased exosome content, and efficacy verification efficacy ratio at composition 5 is close to 100%, demonstrating that the composition of the present invention can significantly solve skin problems.
It is to be understood that this invention is not limited to the particular methodology, protocols, and materials described, as these may vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the present invention which will be limited only by the appended claims.
Those skilled in the art will also recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are encompassed by the appended claims.
Claims (10)
1. Use of a composition comprising a polysaccharide-polypeptide complex for protecting exosome activity; the polysaccharide-polypeptide complex is modified polysaccharide modified by polypeptide; the polysaccharide is hyaluronic acid or sodium salt or potassium salt thereof; the exosomes are mesenchymal stem cell exosomes; the sequence of the polypeptide is Gly-Gly-Gly-Gly-Arg-Gly-Asp-Tyr; the modification percentage of the modified polysaccharide is 35-90%.
2. The use according to claim 1, wherein the mesenchymal stem cell exosomes are selected from bone marrow mesenchymal stem cell exosomes, umbilical cord tissue mesenchymal stem cell exosomes, placenta tissue mesenchymal stem cell exosomes or adipose tissue mesenchymal stem cell exosomes.
3. The use according to claim 1, characterized in that the percentage of modification of the modified polysaccharide is between 55% and 90%.
4. The use of claim 1, wherein the composition further comprises a humectant, a preservative, and water.
5. The use according to claim 4, wherein the humectant is selected from one or more of propylene glycol, butylene glycol, 1, 2-hexanediol, pentylene glycol, β -glucan, glycereth, glycerol, hyaluronic acid or derivatives thereof; the preservative is one or more selected from phenoxyethanol, p-hydroxyacetophenone and octanediol.
6. A skin care composition comprising the polysaccharide-polypeptide complex of any one of claims 1-3, an exosome, a humectant, a preservative, and water; the composition is prepared from the following components in parts by weight: humectant: 1.0-10.0 parts; polysaccharide-polypeptide complex: 0.05-0.5 part; exosomes: 10-50.0 parts; preservative: 0.1-0.3 part; 80-120 parts of purified water; the exosomes are mesenchymal stem cell exosomes.
7. The composition of claim 6, wherein the humectant is selected from one or more of propylene glycol, butylene glycol, 1, 2-hexanediol, pentylene glycol, β -glucan, glycereth, glycerol, hyaluronic acid, or derivatives thereof; the preservative is one or more selected from phenoxyethanol, p-hydroxyacetophenone and octanediol.
8. The composition of claim 6, wherein the composition is prepared from the following components in parts by weight: humectant: 1.5-5.0 parts; polysaccharide-polypeptide complex: 0.05-0.1 part; exosomes: 10-35.0 parts; preservative: 0.1-0.3 part; 90-100 parts of purified water.
9. A process for preparing the composition of claim 6, comprising the steps of:
step 1, respectively weighing the following raw materials in parts by weight: humectant: 1.0-10.0 parts; polysaccharide-polypeptide complex: 0.05-0.5 part; exosomes: 10-50.0 parts; preservative: 0.1-0.3 part; 80-120 parts of purified water; step 2, adding the purified water and the humectant weighed in the step 1 into a reaction vessel, mixing, heating to 75-95 ℃, and uniformly stirring to obtain a phase A; and 3, cooling the phase A to 30-35 ℃, adding the polysaccharide-polypeptide complex, exosomes and preservatives weighed in the step 1 into the phase A obtained in the step 2, and stirring for 20-40 minutes to obtain the polysaccharide-polypeptide complex.
10. Use of the composition of claim 6 in the preparation of a skin care product or cosmetic.
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