CN115317401A - Skin care composition and preparation method and application thereof - Google Patents

Abstract

The present invention relates to a composition for protecting exosome activity, comprising a polysaccharide-polypeptide complex; also relates to a skin care composition stable at normal temperature and a preparation method and application thereof. The composition provided by the invention can preserve exosomes at normal temperature and maintain high activity of the exosomes. The composition has good cell repairing promoting effect, relieving effect, anti-wrinkle and firming effect and capability of remarkably solving various skin problems.

Description

Skin care composition and preparation method and application thereof

Technical Field

The invention belongs to the field of biology, and particularly relates to a composition for protecting the activity of an exosome; also relates to a skin care composition stable at normal temperature and a preparation method and application thereof.

Background

Skin problems are classified into various types, such as skin peeling, color spots, eye bags, dark circles, acne, blackheads, allergy, wrinkles, and the like, which are skin problems. If the skin needs to be well cared, the skin becomes well by paying attention to diet and rest from the aspects of cleaning face, protecting skin and the like, so many functional products such as vitamins, peptides, A alcohols and the like exist on the market at present, the functional raw materials can only stay on the outermost layer of the skin and have very small effect, so a functional raw material which can play a role fundamentally is needed, all the problems of the skin can be attributed to the problems of cells, and the abnormal melanocyte in the skin can occur such as: dull skin, accumulation of pigment; the skin problems are solved by solving the problems of skin cells, such as wrinkles caused by the abnormality of fibroblasts in the skin and skin problems, such as the damage of the skin barrier caused by the abnormality of the horny layer.

Exosomes (exosomes) are small goblet vesicles of bilayer membrane structure with a diameter of about 30-100nm that can be secreted by a variety of cells. The exosome can transfer active substances such as protein, mRNA, microRNA and the like among cells, participate in a plurality of important physiological and pathological processes, and the unique action of the exosome is more and more concerned. Under normal physiological conditions, cells secrete exosomes, which are secreted into the extracellular space by exocytosis as a result of fusion between the plasma membrane and the multivesicular bodies. Exosome-like vesicles are divided into inner and outer portions by a lipid bilayer, which indirectly reflect the properties and state of the cell as they contain membrane lipids, membrane proteins, genetic material and cytosolic components. Meanwhile, the exosome-like vesicles function as extracellular transport proteins mediating cell-cell communication by binding to other cells and tissues and transferring membrane components, mRNA, miRNA, proteins (growth hormone, cytokine, etc.), and the like to recipient cells. Current research shows that exosomes can exert corresponding skin care efficacy by repairing damaged cells.

Skin care products with exosomes added already exist on the market at present, but the difficulty of exosome addition lies in the preservation of activity. It is known to those skilled in the art that exosomes have difficulty ensuring their activity at normal temperature. Therefore, there is a need in the art to develop a functional skin care composition capable of maintaining the activity of exosomes at normal temperature and exerting its efficacy sufficiently.

Disclosure of Invention

Aiming at the defects and problems in the prior art, the invention provides a composition for protecting the activity of exosomes and a preparation method thereof, solves the problem of normal-temperature storage of exosomes in the prior art, and has the advantages of simple production process, strong operability and easy realization.

In a first aspect, the present invention provides a composition for use in preserving exosome activity, characterised in that the composition comprises a polysaccharide-polypeptide complex.

Optionally, the exosome is a mesenchymal stem cell exosome; preferably, the mesenchymal stem cell exosomes are selected from bone marrow mesenchymal stem cell exosomes, umbilical cord blood mesenchymal stem cell exosomes, umbilical cord tissue mesenchymal stem cell exosomes, placenta tissue mesenchymal stem cell exosomes or adipose tissue mesenchymal stem cell exosomes.

Optionally, the polysaccharide-polypeptide complex is a polypeptide-modified polysaccharide or a polypeptide-modified polysaccharide derivative; preferably, the polysaccharide or polysaccharide derivative is at least one of hyaluronic acid or a salt thereof, alginic acid or a salt thereof, hydroxymethyl cellulose or a salt thereof, hydroxypropyl cellulose or a salt thereof, methyl cellulose or a salt thereof, and chitosan or a salt thereof; preferably, the polypeptide is an RGD-containing 3-10 peptide; more preferably, the polypeptide is an RGD-containing octapeptide.

Optionally, the polysaccharide is hyaluronic acid or sodium salt or potassium salt thereof, and preferably, the sequence of the polypeptide is Gly-Gly-Gly-Gly-Arg-Gly-Asp-Tyr.

Bioactive molecules RGD peptide (arginine-glycine-aspartic acid) is used as an important component of natural ECM, and is the most widely applied polypeptide which can promote cell adhesion most effectively at present. The RGD-containing sequence is used for modifying the surface of the polysaccharide material, so that a cell recognition site can be introduced to simulate the extracellular matrix environment of an organism, and the cell affinity of the material is better improved. The polysaccharide-polypeptide compound used in the invention is named as HA-RGD (hydrolyzed sodium hyaluronate-Gly-Gly-Gly-Gly-Arg-Gly-Asp-Tyr). The inventors of the present invention unexpectedly found that such RGD peptide-modified polysaccharide material also has a function of protecting the activity of exosomes at normal temperature, which exerts a cell repair function in a synergistic manner with the composition of exosomes, and verified significant technical advantages of this novel and effective composition in skin care product applications through a plurality of effect verification experiments.

Optionally, the modification percentage of the modified polysaccharide is 35% to 90%; preferably, the percentage modification is from 55% to 90%.

Optionally, the composition further comprises a humectant, a preservative, and water; preferably, the humectant is selected from one or more of propylene glycol, butylene glycol, 1, 2-hexanediol, pentanediol, beta-glucan, glycerol polyether, glycerol, hyaluronic acid or derivatives thereof; the preservative is selected from one or more of phenoxyethanol, p-hydroxyacetophenone and caprylyl glycol.

In another aspect, the present invention provides a skin care composition, wherein the composition comprises the above polysaccharide-polypeptide complex, exosome, humectant, preservative and water; preferably, the exosomes are mesenchymal stem cell exosomes; preferably, the humectant is selected from one or more of propylene glycol, butylene glycol, 1, 2-hexanediol, pentanediol, beta-glucan, glycerol polyether, glycerol, hyaluronic acid or derivatives thereof; the preservative is selected from one or more of phenoxyethanol, p-hydroxyacetophenone and caprylyl glycol.

Optionally, the composition is prepared from the following components in parts by weight:

humectant: 1.0-10.0 parts; polysaccharide-polypeptide complex: 0.001-0.5 parts; exosomes: 10-50.0 parts; preservative: 0.1-0.3 part; 80-120 parts of purified water;

preferably, the composition is prepared from the following components in parts by weight:

humectant: 1.5-5.0 parts; polysaccharide-polypeptide complex: 0.005-0.1 part; exosomes: 10-35.0 parts; preservative: 0.1-0.3 part; 90-100 parts of purified water.

In another aspect, the present invention further provides a preparation method of the above composition, wherein the preparation method comprises the following steps:

step 1, weighing the following raw materials in parts by weight:

humectant: 1.0-10.0 parts; polysaccharide-polypeptide complex: 0.001-0.5 parts; an exosome: 10-50.0 parts; preservative: 0.1-0.3 part; 80-120 parts of purified water;

step 2, adding the purified water and the humectant weighed in the step 1 into a reaction vessel, mixing, heating to 75-95 ℃, and uniformly stirring to obtain a phase A;

and 3, cooling the phase A to 30-35 ℃, adding the polysaccharide-polypeptide compound, the exosome and the preservative weighed in the step 1 into the phase A obtained in the step 2, and stirring for 20-40 minutes to obtain the polypeptide.

In another aspect, the invention also provides the use of the skin care composition of the invention in skin care products or cosmetics. The skin care product or the cosmetic is a common product in the field, and comprises but is not limited to freeze-dried powder, facial mask, smoothing toner, essence, emulsion, cream, cleansing milk and shower gel.

Has the beneficial effects that:

the skin care composition provided by the invention can store exosomes at normal temperature and maintain the high activity of the exosomes. Experiments prove that the composition has good cell repairing promoting effect, relieving effect, anti-wrinkle and firming effect and the capability of remarkably solving various skin problems. The preparation method has the advantages of simple preparation process and low cost, and is suitable for large-scale industrial production. The invention has wide application prospect in the field of cosmetics.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below.

FIG. 1 is a graph comparing the protein content before and after 72 hours for compositions 1-5 of the present invention.

FIG. 2 is a graph showing data on cell proliferation potency of the compositions 1 to 5 of the present invention.

FIG. 3 is a graph of data on the cell migration ability of compositions 1 to 5 of the present invention.

Figures 4 and 5 are graphs of the soothing effect test data for compositions 1-5 of the present invention.

Figure 6 is a graph of wrinkle resistance tightening test data for compositions 1-5 of the present invention.

Detailed Description

The present invention is further described below in conjunction with the following examples, which are intended to be illustrative and explanatory only and are not restrictive of the invention.

Unless defined otherwise, technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. Although methods and materials similar or equivalent to those described herein can be used in the experimental or practical applications, the materials and methods are described below. In case of conflict, the present specification, including definitions, will control. In addition, the materials, methods, and examples are illustrative only and not intended to be limiting.

Example 1

This example provides a method for preparing a skin care composition of the present invention, which comprises raw materials including purified water, glycerin, propylene glycol, exosome, HA-RGD (hydrolyzed sodium hyaluronate-Gly-Arg-Gly-Asp-Tyr), hydrolyzed sodium hyaluronate, and phenoxyethanol.

HA-RGD: purchased from shanxi future polypeptide biotechnology limited, CAS number: 120022-92-6, batch number: 220401. the modification percentage is 55 to 90 percent.

An exosome: is an animal umbilical cord extract, purchased from Guangdong Fujin Stem cell regeneration medicine, inc., and has the following batch number: 220203.

hydrolysis of sodium hyaluronate: purchased from Shandong Anhua, lot No. 211001.

Phenoxyethanol: from sumei, germany, with batch numbers: 210501.

both the glycerol and the propylene glycol are analytically pure.

Preparation of compositions 1-5:

step 1, weighing raw materials (unit: g) according to the following table:

step 2, adding the purified water, the glycerol and the propylene glycol weighed in the step 1 into a pot, mixing, heating to 85 ℃, and uniformly stirring to obtain a phase A;

and 3, cooling to 30-35 ℃, adding the HA-RGD, the exosome and the phenoxyethanol weighed in the step 1 into the phase A obtained in the step 2, and stirring for 30min to obtain the composition 1-5.

Example 2

Protein content testing:

the compositions 1 to 5 prepared in example 1 were left at normal temperature for 72 hours, and the protein contents of the compositions 1 to 5 were measured. The method comprises the following steps: and detecting the concentration of the BCA protein, wherein the adopted kit is purchased for Saimei Fei. If the protein content is basically consistent with the protein content in preparation, which indicates that the loss rate of the protein content is minimum, the activity preservation degree of the exosome is proved to be good; if the loss rate of the protein content is the maximum, the activity of the exosome is not well preserved, so that the corresponding effect is greatly reduced. The results of the experiment are shown in FIG. 1.

As can be seen from fig. 1, in compositions 1 to 5, the loss rate of protein content in composition 2 is the highest after 72 hours, because there is no protective agent, and the loss rate of protein content is sequentially: composition 3, composition 4 and composition 5 show that the protein content loss rate is reduced after the HA-RGD is increased, the capacity of protecting the activity of an exosome is shown, the protein content loss rate of the composition 3 and the composition 4 is obviously reduced along with the increase of the HA-RGD, the protein content loss rate is obviously reduced along with the increase of the HA-RGD in the exosome composition, and the HA-RGD can retain more proteins of the exosome and HAs the function of protecting the integrity and the activity of the exosome.

Example 3

Cell proliferation capacity test:

the growth and development and metabolic cycle of the skin have certain correlation with the activity of skin cells, and the activity of promoting keratinocytes can achieve the purposes of stabilizing skin barriers and promoting skin renewal. The cell activity of the fibroblast is the proportion of healthy cells in a sample group, enhances the cell activity of keratinocytes, and can achieve the purposes of stabilizing skin barriers and promoting skin renewal. Therefore, the cell proliferation capacity can be represented through the cell activity of the fibroblast, and the strong cell proliferation capacity can indicate that the fibroblast has good cell repair effect.

The experimental groups were as follows: the detection model of Shaanxi Boxi general detection technology company Limited is utilized: the fibroblast model is used for detection:

the test results are shown in fig. 2.

As can be seen from the above data, in compositions 1 to 5, the fibroblast viability is, in order: compositions 1 Once 2 Once 3 Once 4 Once 5, composition 2>1 demonstrated that composition 2 exosomes had fibroblast viability-increasing effect; the fibroblast activity of the composition 3 added with HA-RGD is stronger than that of the composition 2 without HA-RGD, which shows that the composition of HA-RGD and exosome HAs stronger effect of promoting cell proliferation. Fibroblast activity of composition 3-composition 4 increased significantly with increasing HA-RGD content, indicating that exosome activity increased with increasing HA-RGD, indicating that the amount of HA-RGD affects exosome activity. Composition 5> composition 4, show that with the increase of the content of exosome, the cell activity is obviously increased, and the composition 5 is very close to positive, and considering the cost factor, the addition of exosome reaches 25% at most, thus not only meeting the effect of cell proliferation and leading to the effect of cell repair, but also saving the cost. The experiment proves that the composition has the obvious cell repair promoting function.

Example 4

Cell migration ability test:

cell migration refers to the movement of a cell upon receiving a migration signal or upon sensing a stimulus from some substance. The cell migration capacity is increased, which shows that the medicine has the function of promoting wound repair. After the skin is damaged, various cells in the skin perform repair activities mainly by cell migration to the damaged part, and the migration ability of keratinocytes plays an important role in repairing damaged epidermis.

The experimental groups were as follows: the detection model of Shaanxi Boxi general detection technology company Limited is utilized: the fibroblast model is used for detection:

the test results are shown in fig. 3.

As can be seen from the above data, in compositions 1-5, fibroblast migration was sequentially: compositions 1 Once 2 Once 3 Once 4 Once 5, composition 2>1 demonstrated that compositions 2 exosomes had fibroblast migration-enhancing effect; the fibroblast migration of composition 3 with the added HA-RGD was stronger than that of composition 2 without the added HA-RGD, indicating that the composition of HA-RGD and exosome HAs stronger effect of promoting cell repair. Fibroblast migration was significantly increased with increased HA-RGD content for compositions 3-4, indicating that exosome activity was increasing with increased HA-RGD, indicating that the amount of HA-RGD affected exosome activity. Composition 5> composition 4, which shows that cell migration is significantly increased with the increase of the content of exosomes, and the cell migration is very close to positive in composition 5, and the addition of exosomes is up to 25% by considering the cost factor, so that the effect of promoting cell repair can be met, and the cost is saved. The experiment proves that the composition has the obvious cell repair promoting function.

Example 5

And (3) testing the relieving effect:

lipopolysaccharide (LPS) is a component of the outer wall of the cell wall of gram-negative bacteria and is a substance (glycolipid) formed by lipid and polysaccharide, and the pathogenic mechanism of pathogenic bacteria is mainly that the LPS is combined with a Toll-like receptor to activate a downstream inflammatory signal pathway, so that an inflammatory reaction is activated, and the inflammatory reaction generates erythema, pain and other clinical phenomena. In the experiment, macrophage is taken as a research model, an in vitro inflammation model is established by adopting LPS stimulation, and the relieving effect of the substance to be detected is analyzed by detecting inflammation related indexes (TNF alpha/IL-1 alpha). TNF alpha/IL 6 is two proinflammatory factors, is mainly mediated and synthesized by a typical signal pathway NF-kB signal pathway, induces inflammatory cascade reaction, and reduces the content of the inflammatory cascade factor, thereby indicating that the sample has a relieving effect. Interleukin 1 α (IL-1 α) is also known as leukocyte pyrogen, leukocyte endogenous mediator, monocyte factor, lymphocyte activator, IL1 α is mature mediated by inflammasome and secreted extracellularly after maturation to further mediate the cascade of inflammatory responses by binding to receptors. The content of the sample is reduced after the action, which shows that the sample has a relieving effect.

The experimental groups were as follows: the detection model of Shaanxi Boxi general detection technology company Limited is utilized:

and (3) detection:

the results of the detection are shown in FIGS. 4 and 5.

As can be seen from the above data, the contents of TNF alpha and IL-1 alpha in the compositions 1-5 are as follows: composition 1> -2 > -3 > -4 > -5, composition 2< 1, indicates that composition 2 exosomes have a reducing effect on both TNF α and IL-1 α, with a soothing effect; the soothing effect of the composition 3 added with HA-RGD is stronger than that of the composition 2 without HA-RGD, which shows that the soothing effect of the composition of HA-RGD and exosome is stronger. The soothing effect of the composition 3-4 is remarkably increased along with the increase of the content of HA-RGD, which shows that the activity of exosomes is increased along with the increase of HA-RGD, and shows that the amount of HA-RGD influences the activity of exosomes. Composition 5 is less than composition 4, which shows that the soothing effect is increased along with the increase of the content of the exosome, the exosome is very close to positive in composition 5, the addition amount of the exosome is up to 25 percent in consideration of the cost, the soothing effect can be met, and the cost is saved. The experiment proves that the composition has obvious relieving function.

Example 6

Anti-wrinkle tightening test:

the experiment takes fibroblasts as a research object, an in-vitro photoaging model is established by UVA irradiation, and the effects of tightening and anti-wrinkle of an object to be tested are analyzed through the change of Collagen I content after the object to be tested is treated. The Collagen I is Collagen I, and the Collagen accounts for about 80% of the proportion of the dermis layer of the skin, so that the skin is full and full, the increase of the Collagen I content is promoted, and the effect of resisting wrinkles can be achieved to a certain extent.

The experimental groups were as follows: the detection model of Shaanxi Boxi general detection technology company Limited is utilized: detection is carried out on Fibroplast:

the results of the detection are shown in FIG. 6.

As can be seen from the above data, the Collagen I contents in the compositions 1-5 are as follows: composition 1-cloth 2-cloth 3-cloth 4-cloth 5, composition 2 >; the composition 3 added with the HA-RGD HAs stronger anti-wrinkle and tightening effects than the composition 2 without the HA-RGD, which shows that the composition of the HA-RGD and exosomes HAs stronger anti-wrinkle and tightening effects. The anti-wrinkle firming effect of the composition 3-composition 4 is remarkably increased along with the increase of the content of HA-RGD, which shows that the activity of exosome is increased along with the increase of HA-RGD, and the amount of HA-RGD influences the activity of exosome. Composition 5 is greater than composition 4, which shows that the anti-wrinkle firming effect is remarkably increased along with the increase of the content of exosomes, the exosomes are close to positive exosomes in composition 5, the addition amount of the exosomes is up to 25% in consideration of the cost, the anti-wrinkle firming effect can be met, and the cost is saved. The experiment proves that the composition has remarkable anti-wrinkle and tightening functions.

Example 7

Testing human body efficacy:

75 volunteers, aged between 28-55 years, were summoned and tested for safety and efficacy in a group of 15 individuals using compositions 1-5, respectively. The safety is mainly completed by testing the human body, and if the volunteer tries out and has no bad feedback, the safety is passed; the effectiveness is tested by an instrument, and main indexes comprise: moisture content, elasticity, wrinkles, melanin content, redness content, TEWL (percutaneous water loss). And testing by a German CK instrument, verifying long-acting effect, carrying out average value processing on effect data after 4 weeks of use, and calculating the improvement degree after nursing, thereby obtaining the proportion of the improved people and the proportion of the non-improved people. The results are as follows: the safety test has no bad feedback proportion of more than 90 percent, and is safe. The judgment basis of the specific indexes of the efficacy verification is as follows: the instrument value is more than 3 after use than before use, the effect is obvious, and the proportion of the number of effective people is more than 50 percent.

As can be seen from the above results, after the four-week long-term test, it was found that by detecting the indicators of moisture content, elasticity, wrinkles, melanin content, rubrin content, TEWL of the subjects after using the composition 3 and the composition 2, it was found that there were long-term effects of anti-wrinkle tightening, skin color lightening, and skin barrier repair, and the long-term effects of anti-wrinkle tightening, skin color lightening, and skin barrier repair of the composition 3 were more significant, indicating that the composition was significantly improved in skin problem solution after adding HA-RGD; the ability of compositions 3 and 4 to solve skin problems increased with increasing HA-RGD content, indicating that the ability to solve skin problems increased with increasing HA-RGD in the compositions, indicating that HA-RGD HAs exosome-protecting activity, making the ability to solve skin problems stronger. Composition 5> composition 4 the ability to solve skin problems was significantly increased with increasing exosome content, and the significant effect ratio was close to 100% in the efficacy test at composition 5, indicating that the compositions of the present invention could significantly solve skin problems.

It is to be understood that the invention disclosed is not limited to the particular methodology, protocols, and materials described, as these may vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the present invention which will be limited only by the appended claims.

Those skilled in the art will also recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are also encompassed by the appended claims.

Claims (10)

1. A composition for use in protecting exosome activity, characterized in that it comprises polysaccharide-polypeptide complexes.

2. The composition of claim 1, wherein the exosomes are mesenchymal stem cell exosomes; preferably, the mesenchymal stem cell exosomes are selected from bone marrow mesenchymal stem cell exosomes, umbilical cord blood mesenchymal stem cell exosomes, umbilical cord tissue mesenchymal stem cell exosomes, placenta tissue mesenchymal stem cell exosomes or adipose tissue mesenchymal stem cell exosomes.

3. The composition of claim 1 or 2, wherein the polysaccharide-polypeptide complex is a polypeptide-modified polysaccharide or a polypeptide-modified polysaccharide derivative; preferably, the polysaccharide or polysaccharide derivative is at least one of hyaluronic acid or a salt thereof, alginic acid or a salt thereof, hydroxymethyl cellulose or a salt thereof, hydroxypropyl cellulose or a salt thereof, methyl cellulose or a salt thereof, and chitosan or a salt thereof; preferably, the polypeptide is an RGD-containing 3-10 peptide; more preferably, the polypeptide is an RGD-containing octapeptide.

4. The composition of claim 3, wherein the polysaccharide is hyaluronic acid or a sodium or potassium salt thereof, preferably wherein the sequence of the polypeptide is Gly-Gly-Gly-Gly-Arg-Gly-Asp-Tyr.

5. The composition of claim 3, wherein the modified polysaccharide has a percent modification of from 35% to 90%; preferably, the modification percentage is 55% to 90%.

6. The composition of claim 1, wherein the composition further comprises a humectant, a preservative, and water; preferably, the humectant is selected from one or more of propylene glycol, butylene glycol, 1, 2-hexanediol, pentanediol, beta-glucan, glycerol polyether, glycerol, hyaluronic acid or derivatives thereof; the preservative is selected from one or more of phenoxyethanol, p-hydroxyacetophenone and caprylyl glycol.

7. A skin care composition comprising the polysaccharide-polypeptide complex of any one of claims 1-5, exosomes, moisturizers, preservatives and water; preferably, the exosomes are mesenchymal stem cell exosomes; preferably, the humectant is selected from one or more of propylene glycol, butylene glycol, 1, 2-hexanediol, pentanediol, beta-glucan, glycerol polyether, glycerol, hyaluronic acid or derivatives thereof; the preservative is selected from one or more of phenoxyethanol, p-hydroxyacetophenone and caprylyl glycol.

8. The composition of claim 7, wherein the composition is prepared from the following components in parts by weight:

humectant: 1.0-10.0 parts; polysaccharide-polypeptide complex: 0.001-0.5 part; exosomes: 10-50.0 parts; preservative: 0.1-0.3 part; 80-120 parts of purified water;

preferably, the composition is prepared from the following components in parts by weight:

humectant: 1.5-5.0 parts; polysaccharide-polypeptide complexes: 0.005-0.1 part; an exosome: 10-35.0 parts; preservative: 0.1-0.3 part; 90-100 parts of purified water.

9. A process for the preparation of a composition according to any one of claims 7 to 8, characterized in that it comprises the following steps:

step 1, weighing the following raw materials in parts by weight:

humectant: 1.0-10.0 parts; polysaccharide-polypeptide complex: 0.001-0.5 parts; exosomes: 10-50.0 parts; preservative: 0.1-0.3 part; 80-120 parts of purified water;

step 2, adding the purified water and the humectant weighed in the step 1 into a reaction vessel, mixing, heating to 75-95 ℃, and uniformly stirring to obtain a phase A;

and 3, cooling the phase A to 30-35 ℃, adding the polysaccharide-polypeptide compound, the exosome and the preservative weighed in the step 1 into the phase A obtained in the step 2, and stirring for 20-40 minutes to obtain the polypeptide.

10. Use of a composition according to any one of claims 7 to 9 in the preparation of a skin care or cosmetic product.

Images