CN115215432B - Granular sludge suitable for town sewage treatment and rapid generation method thereof - Google Patents
Granular sludge suitable for town sewage treatment and rapid generation method thereof Download PDFInfo
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
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- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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Abstract
The invention discloses granular sludge suitable for town sewage treatment and a rapid generation method thereof. The method comprises the following steps: adding candida utilis and lactobacillus fermentum into dehydrated sludge of town sewage plants, uniformly stirring, and standing for culturing; continuously adding the compound binder and the mussel bionic powder active carbon, and uniformly stirring to form granular sludge; immersing the granular sludge in a modified solution of ammonium molybdate and boric acid, stirring uniformly, and repeatedly cleaning with tap water to obtain modified granular sludge; adding the modified granular sludge into a disc granulator, spraying corn flour, rolling and sieving to obtain pre-granulated aerobic granular sludge. The invention can effectively reduce the preparation period of the aerobic granular sludge, greatly shortens the culture and domestication period compared with the traditional granular sludge, can realize excellent denitrification and dephosphorization effects, obviously improves the sedimentation performance of the granular sludge and reduces the particle roughness. The technology has the characteristics of simplicity, high efficiency, easy popularization and the like.
Description
Technical Field
The invention belongs to the field of water treatment, and particularly relates to granular sludge suitable for town sewage treatment and a rapid generation method thereof.
Background
Aerobic granular sludge is granular biological aggregate formed by spontaneous agglomeration of microorganisms in a specific environment. The sewage treatment device has a relatively compact microstructure, excellent sedimentation performance, high-concentration sludge retention and various microorganism populations, can bear large impact load, has high unit volume treatment capacity, and can reduce the occupied area of a sewage plant. In 2000, aerobic granular sludge gradually becomes a research hot spot in the field of sewage treatment, and researches are conducted on the basic conditions, influencing factors, denitrification and dephosphorization effects and the like of the formation of the aerobic granular sludge, so that the formation and action mechanisms of the aerobic granular sludge are deeply analyzed. In the operation process, the aerobic granular sludge has the problems of long formation time, unstable operation and the like, and researchers aim at the problems to provide solutions of optimizing operation parameters, improving a reactor, strengthening granulation and the like, but still one to three months are required to form the aerobic granular sludge in the reactor, and part of the methods are not suitable for practical sewage plants and are difficult to use on a large scale.
The research shows that the addition of some binders in the sludge can effectively promote the sludge to be agglomerated into particles, the particles are assisted to form the granular sludge, and the particles have better sedimentation performance and mechanical strength, strong impact load resistance and good operation stability. The carrageenan, alginate, locust bean gum, guar gum and other hydrophilic colloids have good gel synergistic effect, and can be compounded into a new binder, so that the dosage of the hydrophilic colloids is reduced, the product quality is improved, and the production cost is reduced.
The sewage contains various harmful substances including various cationic pollutants such as methylene blue, methyl violet and the like. These toxin contaminants are stably present in the wastewater and increase the difficulty of removal. Researchers found that mussels possess the ability to adhere with high strength in strong ocean currents, and their secreted podites consist of a variety of adhesion proteins, all of which contain dopa, with good adhesion in wet environments. Based on the porous structure of the sponge and the bionic chemical principle of mussels, the functional polyurethane sponge capable of efficiently removing cationic pollutants in sewage can be prepared.
In the sewage treatment process, saccharomycetes can adsorb and remove phosphorus, wherein the candida albovinosa plays an important role in the biological phosphorus removal process. During the phosphorus metabolism of candida utilis, phosphorus is mainly stored in both extracellular polymer (Extracellular Polymeric Substances, EPS) and intracellular sites. The signal molecules play an important role in promoting sludge granulation and maintaining particle stability. Experiments show that strains such as lactobacillus fermentum and the like can generate a large number of signal molecules AI-2, regulate genes, promote EPS formation, increase cell viscosity, and accelerate microorganism aggregation to form aerobic granular sludge (Aerobic Granular Sludge, AGS).
Disclosure of Invention
Aiming at the problems of longer sludge granulation time and poor removal effect in the initial operation stage, the invention provides granular sludge suitable for town sewage treatment and a rapid generation method thereof. According to the method, candida utilis, lactobacillus fermentum and dehydrated sludge are mixed for the first time, and the sludge is prepared into particles by compounding a binder and mussel bionic powder active carbon. Compared with the conventionally formed granular sludge, the granular sludge prepared by the method has higher mechanical strength and adsorption performance and lower particle roughness. In addition, the modification of ammonium molybdate and boric acid promotes microorganisms on the granular sludge to secrete more EPS, so that the dephosphorization effect is enhanced, and the process operation stability is improved. Finally, the grain strength of the sludge is further consolidated and enhanced through the corn flour wrapping, and meanwhile, a carbon source is provided for microorganisms in the granular sludge, so that the long-term efficient operation of the granular sludge in the sewage treatment process is realized, and the sewage treatment efficiency of the granular sludge technology is remarkably improved.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
the granular sludge suitable for town sewage treatment and the rapid generation method thereof comprise the following steps:
(1) Taking dewatered sludge of town sewage plants as a basic material;
(2) Adding candida utilis and lactobacillus fermentum into the dehydrated sludge, uniformly stirring, and then standing, culturing and fermenting;
(3) After fermentation, adding a compound binder and mussel bionic powder active carbon, uniformly stirring, and cutting by a screen and rolling by a disc to form granular sludge;
(4) Immersing the granular sludge obtained in the step (3) in a modified solution of ammonium molybdate and boric acid, uniformly stirring, and repeatedly cleaning with water to obtain modified granular sludge;
(5) Adding the modified granular sludge into corn flour, rolling to uniformly coat the surface of the granular sludge, and sieving to obtain pre-granulated aerobic granular sludge;
the mussel bionic powder active carbon is prepared by introducing double bond functional groups on the surface of the powder active carbon through a mussel bionic technology and then modifying sulfonic groups on the surface of the active carbon through an in-situ polymerization method.
Preferably, in the step (1), the water content of the dewatered sludge of the town sewage plant is 65-85%.
Preferably, in the step (2), the arkins usedThe spore yeast is yeast separated and preserved in the sewage treatment system, and the method for preserving the yeast is disclosed in patent publication No. CN104928182A. Activating and enriching the candida utilis in YPD culture medium to obtain a yeast suspension for expanded culture, wherein the adding amount is 0.5% -10% (v/v), and the adding amount represents that 0.5-10mL of the yeast suspension is inoculated into every 100mL of dehydrated sludge, and the OD of the yeast in the yeast suspension 600 The value is 0.4-2.3.
Preferably, the specific operation steps of activating, enriching and expanding the candida utilis in the YPD culture medium comprise: inoculating the candida utilis into YPD solid culture medium, and culturing at 20-30 ℃ for 48-72h; inoculating activated candida utilis into YPD liquid culture medium, and culturing at 20-30 ℃ for 24-48h; after the culture is finished, the test tube culture solution is gently and uniformly shaken, all the test tube culture solution is connected into a triangular flask, and then is cultured for 24 to 36 hours at the temperature of 20 to 25 ℃; and slightly and uniformly shaking the triangular flask after the culture is finished, and then, completely inoculating the triangular flask into the large triangular flask, and culturing for 24-36 hours at 20-25 ℃.
Preferably, in the step (2), the lactobacillus fermentum is purchased from Shanghai biological technology Co., ltd, inoculated into MRS culture medium for activation culture to obtain fermentation liquor with growth log phase, the dosage is 0.5% -10% (v/v), which means that 0.5-10mL of bacterial suspension is inoculated in every 100mL of dehydrated sludge, and OD of the lactobacillus fermentum in the bacterial suspension 600 The value is 0.6-3.6.
Preferably, the lactobacillus fermentum is inoculated in a sterilized MRS culture medium, and is subjected to activation culture for three generations at 37 ℃, each generation is cultured for 24-36 hours, and fermentation liquor growing to a logarithmic phase in the third generation is used for inoculation.
Preferably, the stationary culture fermentation in the step (2) is to ferment the obtained mixed sludge at the temperature of 10-35 ℃ for 2-24 hours.
Preferably, in the step (3), the compound binder is selected from two or more of I-carrageenan, alginate, locust bean gum and guar gum, and the addition amount is 1% -25% of the mass of the dehydrated sludge.
Preferably, in the step (3), the mussel bionic powder activated carbon is prepared by introducing double bond functional groups on the surface of the powder activated carbon and then modifying sulfonic groups on the surface of the activated carbon by an in-situ polymerization method. The preparation method specifically comprises the following steps: adding a proper amount of 3, 4-dihydroxyphenethyl-acrylamide and dopamine hydrochloride into methanol, uniformly stirring, regulating the pH to 8-9 by using trihydroxy aminomethane, completely immersing the powdered activated carbon in the solution, oscillating for 12-24 hours at 25-30 ℃, then taking out the activated carbon and flushing with deionized water for 3 times to obtain the powdered activated carbon with double bond function, immersing the powdered activated carbon with double bond function in an aqueous solution of sodium p-vinylsulfonate (SPS) and Ammonium Persulfate (APS), oscillating and immersing for 0.5-3 hours, polymerizing for 12-18 hours at 80-100 ℃, taking out the activated carbon, cleaning for several times by using deionized water, oscillating and immersing for 0.5-3 hours, and cleaning for several times to obtain the mussel bionic powdered activated carbon.
Preferably, the particle size of the powdered activated carbon is 10-200 mu m, and the specific surface area is 800-1300m 2 /g。
Preferably, the adding amount of the mussel bionic powder active carbon is 1-20% by volume of dehydrated sludge, and the stirring time is 0.1-20h.
Preferably, in the step (3), the sludge is extruded by a granulator and passes through a sludge sieve with the aperture of 1-2mm, cut into cylindrical sludge by a slicer, and then rolled into spherical granular sludge by a disc granulator, wherein the rotating speed of the disc granulator is 30-60r/min, and the inclination angle is 30-60 degrees.
Preferably, in the step (4), the concentration of the ammonium molybdate solution is 15-50mg/L, the concentration of the boric acid solution is 10-40mg/L, and the mixture is stirred for 10-30 hours at 20-35 ℃.
Preferably, in the step (5), the grain size of the corn flour is 1-200 μm.
Preferably, the specific operation of step (5) comprises: adding the granular sludge into a disc granulator, uniformly spraying corn flour, enabling the rotation speed of the granulator to be 30-60r/min, enabling the inclination angle to be 30-60 degrees, enabling the granular sludge to pass through a screen with the aperture of 0.2-0.9mm after rolling for 10-100 min.
Preferably, in the step (5), the roughness is obtained by analyzing the mean square error of the radius of the particle projection equivalent circle and the particle actual boundary contour projection by using an imager, and is generally less than 0.15Ra.
The invention also provides the granular sludge which is prepared by the preparation method and is suitable for town sewage treatment.
The invention also provides a sewage treatment method, which adopts the granular sludge suitable for town sewage treatment to treat.
The invention also provides application of the granular sludge suitable for town sewage treatment in the environmental field.
The beneficial effects of the invention are mainly reflected in that:
(1) The granular sludge prepared by the invention is directly prepared by mixing the compound binder and the sludge, and a proper amount of mussel bionic powder activated carbon is added to improve the adsorption performance of the granular sludge. The compound binder consists of various hydrophilic colloids, is simple to prepare, has the functions of agglomerating sludge, providing carbon sources, promoting EPS secretion and the like, is beneficial to improving the denitrification and dephosphorization effects of a granular sludge system, and enhances the long-term operation stability. The powder activated carbon is modified by sulfonic groups by referring to key components in liquid protein secreted when mussel foot glands are adhered to solids, so that the adsorption of harmful substances in sewage is obviously enhanced, the interaction of granular sludge and pollutants is effectively promoted, and the sewage treatment efficiency is improved.
(2) The granular sludge prepared by the invention contains the candida utilis, has a strong absorption and conversion effect on phosphorus, the main phosphorus storage part of the candida utilis is EPS and intracellular, and the addition of lactobacillus fermentum is beneficial to secreting more AI-2, so that the stability of the granular sludge is enhanced; the ammonium molybdate solution is added for modification, so that the ammonium molybdate solution can promote the ammonium molybdate solution to secrete more EPS, the adsorption and storage capacity of phosphorus is improved, the ammonium molybdate solution is soaked in the boric acid solution, the formation of an AI-2 precursor can be promoted, the activity of AI-2 is obviously enhanced, the EPS is promoted to be formed, and the viscosity of the granular sludge is enhanced.
(3) The particles can be directly operated when being put into a sewage treatment system, and the method has the advantages of convenient operation, low roughness, good sedimentation and mud-water separation performance, and good pollutant removal effect at the initial stage of operation.
Detailed Description
The following describes the content of the present invention in further detail by way of examples, but the embodiments of the present invention are not limited thereto.
Example 1:
(1) 100mL of dewatered sludge of town sewage plants with the water content of about 80% is taken.
(2) Activating and enriching the candida utilis in a YPD culture medium, taking a saccharomycete aggregate in a logarithmic phase during activation, filtering the saccharomycete aggregate by using sterile gauze, and cleaning the saccharomycete aggregate by using sterile normal saline for 3 times to obtain saccharomycete suspension. Lactobacillus fermentum was purchased from Shanghai, highway Biotechnology, inc., inoculated into sterilized MRS medium, and subjected to activation culture at 37deg.C for three generations, each generation being cultured for 24 hr, and the fermentation broth grown from the third generation to logarithmic phase was used for inoculation.
(3) 5mL of the bacterial suspension and 2mL of the fermentation broth are added into 100mL of dehydrated sludge, the mixture is stirred for 15min to be fully mixed, and the mixture is subjected to stationary culture at 20 ℃ for 2h.
(4) Respectively weighing 8g I-carrageenan, 8g sodium alginate and 4g guar gum to compound into a binder; weighing 5g of powdered activated carbon, adding 1g of 3, 4-dihydroxyphenethyl-acrylamide and 0.2g of dopamine hydrochloride into 500mL of methanol, stirring uniformly, regulating the pH to 8-9 by using trihydroxy aminomethane, completely immersing the powdered activated carbon in the solution, oscillating for 12 hours at 25 ℃, then taking out the activated carbon, and flushing with deionized water for 3 times to obtain the powdered activated carbon with double bond function. Immersing double bond functional powder active carbon in a mixed aqueous solution of sodium p-vinyl sulfonate (SPS) with the mass fraction of 10% and Ammonium Persulfate (APS) with the mass fraction of 0.1%, carrying out oscillation soaking for 0.5h, then polymerizing at 80 ℃ for 12 h, taking out the active carbon, washing with deionized water for 3 times, carrying out oscillation soaking for 0.5h, and washing for 3 times to obtain the mussel bionic powder active carbon.
(5) Adding the compound binder and the mussel bionic powder active carbon into the dehydrated sludge, and uniformly stirring for 20min to fully mix the materials. And extruding the obtained sludge to pass through a screen with the aperture of 1.3mm, sieving and cutting the sludge at a constant speed to obtain cylindrical sludge with the bottom surface diameter of 1.3mm and the height of 1.3 mm. Setting the rotating speed of the disc granulator to 45r/min and the inclination angle to 55 degrees, adding the columnar sludge into the disc granulator, rotating for 15min, and enabling the granular sludge to become spherical.
(6) Preparing a mixed solution of 20mg/L ammonium molybdate and 15mg/L boric acid, immersing the granular sludge in the solution, stirring for 20 hours at 25 ℃, taking out, cleaning for 3 times by using tap water, and immersing for 12 hours to obtain the modified granular sludge.
(7) Setting the rotating speed of the disc granulator to 45r/min, setting the inclination angle to 55 degrees, putting the modified granular sludge into the disc granulator, starting to spray corn flour after rotating for 3min, continuing to roll to uniformly coat the corn flour on the surface of the granular sludge, stopping after running for 15min, and passing the sludge in the disc through a screen with the aperture of 0.5mm to obtain the pre-granulated granular sludge. Analyzing the radius mean square error of the particle projection equivalent circle and the particle actual boundary contour projection by adopting an image instrument to obtain roughness C R ＝0.007Ra。
Example 2:
(1) Taking 100L of dewatered sludge of town sewage plants with the water content of about 80 percent.
(2) Activating and enriching the candida utilis in a YPD culture medium, taking a saccharomycete aggregate in a logarithmic phase during activation, filtering the saccharomycete aggregate by using sterile gauze, and cleaning the saccharomycete aggregate by using sterile normal saline for 3 times to obtain saccharomycete suspension. Lactobacillus fermentum was purchased from Shanghai, highway Biotechnology, inc., inoculated into sterilized MRS medium, and subjected to activation culture at 37deg.C for three generations, each generation being cultured for 24 hr, and the fermentation broth grown from the third generation to logarithmic phase was used for inoculation.
(3) 0.5L of bacterial suspension and 0.5L of fermentation liquor are added into 100L of dehydrated sludge, stirred for 30min to be fully mixed, and the mixture is subjected to static culture for 12h at 20 ℃.
(4) Respectively weighing 0.3kg of I-type carrageenan, 0.3kg of sodium alginate, 0.2kg of locust bean gum and 0.2kg of guar gum to compound into a binder, weighing 5kg of powdered activated carbon, adding 10g of 3, 4-dihydroxyphenethyl-acrylamide and 2g of dopamine hydrochloride into 20L of methanol, uniformly stirring, regulating the pH to 8-9 by using trihydroxy aminomethane, completely immersing the powdered activated carbon in the solution, oscillating for 12 hours at 25 ℃, then taking out the activated carbon and flushing with deionized water for 3 times to obtain the powdered activated carbon with double bond function. Immersing double bond functional powder active carbon in a mixed aqueous solution of sodium p-vinyl sulfonate (SPS) with the mass fraction of 10% and Ammonium Persulfate (APS) with the mass fraction of 0.1%, carrying out oscillation soaking for 0.5h, then polymerizing at 80 ℃ for 12h, taking out the active carbon, washing with deionized water for 3 times, carrying out oscillation soaking for 0.5h, and washing for 3 times to obtain the mussel bionic powder active carbon.
(5) Adding the compound binder and the mussel bionic powder active carbon into the dehydrated sludge, and uniformly stirring for 3 hours to fully mix the dehydrated sludge. And extruding the obtained sludge to pass through a screen with the aperture of 1.3mm, sieving and cutting the sludge at a constant speed to obtain cylindrical sludge with the bottom surface diameter of 1.3mm and the height of 1.3 mm. Setting the rotating speed of the disc granulator to 45r/min, setting the inclination angle to 55 degrees, adding the columnar sludge into the disc granulator, rotating for 1.5h, and enabling the granular sludge to be spherical.
(6) Preparing a mixed solution of 15mg/L ammonium molybdate and 10mg/L boric acid, immersing the granular sludge in the solution, stirring for 24 hours at 25 ℃, taking out, cleaning for 3 times by using tap water, and immersing for 12 hours to obtain the modified granular sludge.
(7) Setting the rotating speed of the disc granulator to 45r/min, setting the inclination angle to 55 degrees, putting the modified granular sludge into the disc granulator, starting to spray corn flour after rotating for 10min, continuing to roll to uniformly coat the corn flour on the surface of the granular sludge, stopping after running for 1h, and passing the sludge in the disc through a screen with the aperture of 0.5mm to obtain the pre-granulated granular sludge. Analyzing the radius mean square error of the particle projection equivalent circle and the particle actual boundary contour projection by adopting an image instrument to obtain roughness C R ＝0.008Ra。
Example 3
(1) Taking 100L of dewatered sludge of town sewage plants with the water content of about 80 percent.
(2) Activating and enriching the candida utilis in a YPD culture medium, taking a saccharomycete aggregate in a logarithmic phase during activation, filtering the saccharomycete aggregate by using sterile gauze, and cleaning the saccharomycete aggregate by using sterile normal saline for 3 times to obtain saccharomycete suspension. Lactobacillus fermentum was purchased from Shanghai, highway Biotechnology, inc., inoculated into sterilized MRS medium, and subjected to activation culture at 37deg.C for three generations, each generation being cultured for 24 hr, and the fermentation broth grown from the third generation to logarithmic phase was used for inoculation.
(3) 10L of bacterial suspension and 10L of fermentation liquor are added into 100L of dehydrated sludge, stirred for 30min to be fully mixed, and the mixture is subjected to static culture for 12h at 20 ℃.
(4) 6kg of I-carrageenan, 6kg of sodium alginate, 6kg of locust bean gum and 7kg of guar gum are respectively weighed to be compounded into a binder, 10kg of powdered activated carbon is weighed, 10g of 3, 4-dihydroxyphenethyl-acrylamide and 2g of dopamine hydrochloride are added into 20L of methanol and are uniformly stirred, the pH is regulated to 8-9 by using trihydroxy aminomethane, the powdered activated carbon is completely immersed in the solution, the solution is oscillated for 24 hours at 30 ℃, and then the powdered activated carbon with double bond function is obtained by taking out the activated carbon and flushing the activated carbon with deionized water for 3 times. Immersing double bond functional powder active carbon in a mixed aqueous solution of sodium p-vinyl sulfonate (SPS) with the mass fraction of 10% and Ammonium Persulfate (APS) with the mass fraction of 0.1%, oscillating and soaking for 3 hours, polymerizing at 100 ℃ for 18 hours, taking out the active carbon, cleaning with deionized water for 3 times, oscillating and soaking for 3 hours, and cleaning for 3 times to obtain the mussel bionic powder active carbon.
(5) Adding the compound binder and the mussel bionic powder active carbon into the dehydrated sludge, and uniformly stirring for 20 hours to fully mix the materials. And extruding the obtained sludge to pass through a screen with the aperture of 1.3mm, sieving and cutting the sludge at a constant speed to obtain cylindrical sludge with the bottom surface diameter of 1.3mm and the height of 1.3 mm. Setting the rotating speed of the disc granulator to 45r/min, adding columnar sludge into the disc granulator, rotating for 6h, and enabling the granular sludge to become spherical.
(6) Preparing a mixed solution of 50mg/L ammonium molybdate and 40mg/L boric acid, immersing the granular sludge in the solution, stirring for 30 hours at 35 ℃, taking out, cleaning for 3 times by using tap water, and immersing for 12 hours to obtain the modified granular sludge.
(7) Setting the rotating speed of a disc granulator to 45r/min, setting the inclination angle to 55 degrees, putting the modified granular sludge into the disc granulator, starting to spray corn flour after rotating for 0.5h, continuing to roll to uniformly coat the corn flour on the surface of the granular sludge, stopping after running for 1.5h, and passing the sludge in the disc through a screen with the aperture of 0.5mm to obtain the pre-granulated granular sludge. Analyzing the radius mean square error of the particle projection equivalent circle and the particle actual boundary contour projection by adopting an image instrument to obtain roughness C R ＝0.0065Ra。
Example 4
(1) Taking 10L of dewatered sludge of town sewage plants with the water content of about 80 percent.
(2) Activating and enriching the candida utilis in a YPD culture medium, taking a saccharomycete aggregate in a logarithmic phase during activation, filtering the saccharomycete aggregate by using sterile gauze, and cleaning the saccharomycete aggregate by using sterile normal saline for 3 times to obtain saccharomycete suspension. Lactobacillus fermentum was purchased from Shanghai, highway Biotechnology, inc., inoculated into sterilized MRS medium, and subjected to activation culture at 37deg.C for three generations, each generation being cultured for 24 hr, and the fermentation broth grown from the third generation to logarithmic phase was used for inoculation.
(3) 0.3L of bacterial suspension and 0.5L of fermentation liquor are added into 10L of dehydrated sludge, stirred for 30min to be fully mixed, and the mixture is subjected to static culture for 12h at 20 ℃.
(4) Respectively weighing 0.5kg of I-type carrageenan, 0.5kg of sodium alginate, 0.5kg of locust bean gum and 0.5kg of guar gum to compound into a binder, weighing 1kg of powdered activated carbon, adding 1g of 3, 4-dihydroxyphenethyl-acrylamide and 0.2g of dopamine hydrochloride into 2L of methanol, uniformly stirring, regulating pH to 8-9 by using trihydroxy aminomethane, completely immersing the powdered activated carbon in the solution, oscillating for 18 hours at 25 ℃, then taking out the activated carbon and flushing for 3 times by using deionized water to obtain the powdered activated carbon with double bond function. Immersing double bond functional powder active carbon in a mixed aqueous solution of sodium p-vinyl sulfonate (SPS) with the mass fraction of 10% and Ammonium Persulfate (APS) with the mass fraction of 0.1%, carrying out oscillation soaking for 1h, then polymerizing at 80 ℃ for 16 h, taking out the active carbon, washing with deionized water for 3 times, carrying out oscillation soaking for 1h, and washing for 3 times to obtain the mussel bionic powder active carbon.
(5) Adding the compound binder and the mussel bionic powder active carbon into the dehydrated sludge, and uniformly stirring for 15h to fully mix the dehydrated sludge. And extruding the obtained sludge to pass through a screen with the aperture of 1.3mm, sieving and cutting the sludge at a constant speed to obtain cylindrical sludge with the bottom surface diameter of 1.3mm and the height of 1.3 mm. Setting the rotating speed of the disc granulator to 45r/min, adding columnar sludge into the disc granulator, rotating for 2 hours, and enabling the granular sludge to become spherical.
(6) Preparing a mixed solution of 20mg/L ammonium molybdate and 20mg/L boric acid, immersing the granular sludge in the solution, stirring for 20 hours at 30 ℃, taking out, cleaning for 3 times by using tap water, and immersing for 12 hours to obtain the modified granular sludge.
(7) Setting the rotating speed of the disc granulator to 45r/min and the inclination angle to 55 degrees, putting the modified granular sludge into the disc granulator, starting to spray corn flour after rotating for 0.5h, and thenAnd (3) continuously rolling to uniformly coat the sludge on the surface of the granular sludge, stopping after running for 1.5 hours, and enabling the sludge in the disc to pass through a screen with the aperture of 0.5mm to obtain the pre-granulated granular sludge. Analyzing the radius mean square error of the particle projection equivalent circle and the particle actual boundary contour projection by adopting an image instrument to obtain roughness C R ＝0.008Ra。
Comparative example 1
The preparation method of the common granular sludge comprises the following steps:
(1) Taking 100mL of dewatered sludge of the town sewage plant with the water content of about 80%, and carrying out vacuum dehydration until the water content is 60%.
(2) And extruding the obtained sludge to pass through a screen with the aperture of 1.3mm, sieving and cutting the sludge at a constant speed to obtain cylindrical sludge with the bottom surface diameter of 1.3mm and the height of 1.3 mm. Setting the rotating speed of the disc granulator to 45r/min, setting the inclination angle to 55 degrees, adding the columnar sludge into the disc granulator, and rotating for 2 hours to obtain the spherical granular sludge. And analyzing the radius mean square error of the particle projection equivalent circle and the particle actual boundary contour projection by adopting an image instrument to obtain the roughness of about 0.01Ra.
Test case
The pre-granulated granular sludge of example 2 and comparative example 1 was added to an AGS reaction tank, which was operated in water inlet, aeration, precipitation, water outlet and idle mode with a period of 4h: 0.8h of water inflow, 2h of aeration, 0.2h of precipitation, 0.8h of water outflow and 0.2h of idle time, wherein COD (chemical oxygen demand) of the water inflow is 600mg/L, total nitrogen is 40mg/L, ammonia nitrogen is 22mg/L, and total phosphorus is 5mg/L.
After 10 days of operation, the granular sludge added into the pre-granular sludge AGS reaction tank of example 2 has a good running state, and the average grain size of the sludge is 1.43mm. The SVI is calculated by dividing the sludge volume (mL) of 1L of muddy water mixed solution after 5min and 30min precipitation by the dry weight (g) of the sludge 5 And SVI 30 Within a certain range, SVI 5 And SVI 30 Smaller represents better granulation. The invention obtains SVI of granular sludge 5 And SVI 30 25mL/g and 23mL/g, respectively, and SVI 30 /SVI 5 0.92 SVI 30 Compared with the common granular sludge, the sludge is reduced by 41%, has better sedimentation performance and has roughness of about 0.013Ra.
After 40 days of operation, a comparison is addedThe operation state of the granular sludge in the pre-granular sludge AGS reaction tank of example 1 was good, the average particle size of the sludge was 0.9mm, SVI 5 43mL/g SVI 30 39mL/g SVI 30 /SVI 5 The roughness was 0.91, approximately 0.017Ra, 31% improvement over example 2.
As can be seen from the measured water quality, the COD of the effluent obtained by adding the pre-granulated granular sludge of the example 2 is 40mg/L, the total nitrogen is 8.94mg/L, the ammonia nitrogen is 0.8mg/L, the total phosphorus is 0.35mg/L, and the water quality of the effluent reaches the first-level A standard at the 4 d. The effluent of the common granular sludge in comparative example 1 reaches the first grade A standard at 36d, and the standard reaching time is delayed by 32d. After 40 days of operation, compared with the common granular sludge of comparative example 1, the total nitrogen removal rate of effluent of the pre-granulated granular sludge of example 2 is improved by about 10%, the total phosphorus removal rate is improved by about 25%, and the aerobic granular sludge can still keep stable structure after 90 days of operation.
Comparative example 2
The mussel bionic powder active carbon in the step (4) is changed into common active carbon, and the rest of the operations are the same as in the example 2.
(1) Taking 100L of dewatered sludge of town sewage plants with the water content of about 80 percent.
(2) Activating and enriching the candida utilis in a YPD culture medium, taking a saccharomycete aggregate in a logarithmic phase during activation, filtering the saccharomycete aggregate by using sterile gauze, and cleaning the saccharomycete aggregate by using sterile normal saline for 3 times to obtain saccharomycete suspension. Lactobacillus fermentum was purchased from Shanghai, highway Biotechnology, inc., inoculated into sterilized MRS medium, and subjected to activation culture at 37deg.C for three generations, each generation being cultured for 24 hr, and the fermentation broth grown from the third generation to logarithmic phase was used for inoculation.
(3) 0.5L of bacterial suspension and 0.5L of fermentation liquor are added into 100L of dehydrated sludge, stirred for 30min to be fully mixed, and the mixture is subjected to static culture for 12h at 20 ℃.
(4) Respectively weighing 0.3kg of I-carrageenan, 0.3kg of sodium alginate, 0.2kg of locust bean gum and 0.2kg of guar gum to compound into a binder, and weighing 5kg of powdered activated carbon.
(5) Adding the compound binder and the powdered activated carbon into the dehydrated sludge, and uniformly stirring for 3 hours to fully mix the materials. And extruding the obtained sludge to pass through a screen with the aperture of 1.3mm, sieving and cutting the sludge at a constant speed to obtain cylindrical sludge with the bottom surface diameter of 1.3mm and the height of 1.3 mm. Setting the rotating speed of the disc granulator to 45r/min, setting the inclination angle to 55 degrees, adding the columnar sludge into the disc granulator, rotating for 1.5h, and enabling the granular sludge to be spherical.
(6) Preparing a mixed solution of 15mg/L ammonium molybdate and 10mg/L boric acid, immersing the granular sludge in the solution, stirring for 24 hours at 25 ℃, taking out, cleaning for 3 times by using tap water, and immersing for 12 hours to obtain the modified granular sludge.
(7) Setting the rotating speed of the disc granulator to 45r/min, setting the inclination angle to 55 degrees, putting the modified granular sludge into the disc granulator, starting to spray corn flour after rotating for 10min, continuing to roll to uniformly coat the corn flour on the surface of the granular sludge, stopping after running for 1h, and passing the sludge in the disc through a screen with the aperture of 0.5mm to obtain the pre-granulated granular sludge.
The water treatment effect of the granular sludge prepared in comparative example 2 was tested according to the test method of example 2, and found that the granular sludge in the AGS reaction tank for pre-granulated granular sludge added in comparative example 2 had a good running state after 10 days of running, the average particle size of the sludge was 1.2mm, and SVI 5 30mL/g SVI 30 27mL/g SVI 30 /SVI 5 0.92 SVI 30 The sedimentation performance was deteriorated by 15% as compared with the granular sludge of example 2, and the roughness was about 0.014Ra, which was 8% as compared with example 2. The COD of the effluent is 41mg/L, the total nitrogen is 9.23mg/L, the ammonia nitrogen is 0.8mg/L, the total phosphorus is 0.38mg/L, and the quality of the effluent reaches the first grade A standard in the 5 th day. After 40 days of operation, the effluent quality of the granular sludge of comparative example 2 was slightly reduced compared with the pre-granulated granular sludge of example 2, and the aerobic granular sludge remained structurally stable after 90 days of operation.
Comparative example 3
The procedure of example 2 was repeated except that the Candida utilis in step (2) was omitted.
(1) Taking 100L of dewatered sludge of town sewage plants with the water content of about 80 percent.
(2) Lactobacillus fermentum was purchased from Shanghai, highway Biotechnology, inc., inoculated into sterilized MRS medium, and subjected to activation culture at 37deg.C for three generations, each generation being cultured for 24 hr, and the fermentation broth grown from the third generation to logarithmic phase was used for inoculation. Adding 0.5L of fermentation liquor into 100L of dehydrated sludge, stirring for 30min to fully mix, and standing and culturing for 12h at 20 ℃.
(3) Respectively weighing 0.3kg of I-type carrageenan, 0.3kg of sodium alginate, 0.2kg of locust bean gum and 0.2kg of guar gum to compound into a binder, weighing 5kg of powdered activated carbon, adding 10g of 3, 4-dihydroxyphenethyl-acrylamide and 2g of dopamine hydrochloride into 20L of methanol, uniformly stirring, regulating the pH to 8-9 by using trihydroxy aminomethane, completely immersing the powdered activated carbon in the solution, oscillating for 12 hours at 25 ℃, then taking out the activated carbon and flushing with deionized water for 3 times to obtain the powdered activated carbon with double bond function. Immersing double bond functional powder active carbon in a mixed aqueous solution of sodium p-vinyl sulfonate (SPS) with the mass fraction of 10% and Ammonium Persulfate (APS) with the mass fraction of 0.1%, carrying out oscillation soaking for 0.5h, then polymerizing at 80 ℃ for 12h, taking out the active carbon, washing with deionized water for 3 times, carrying out oscillation soaking for 0.5h, and washing for 3 times to obtain the mussel bionic powder active carbon.
(4) Adding the compound binder and the mussel bionic powder active carbon into the dehydrated sludge, and uniformly stirring for 3 hours to fully mix the dehydrated sludge. And extruding the obtained sludge to pass through a screen with the aperture of 1.3mm, sieving and cutting the sludge at a constant speed to obtain cylindrical sludge with the bottom surface diameter of 1.3mm and the height of 1.3 mm. Setting the rotating speed of the disc granulator to 45r/min, setting the inclination angle to 55 degrees, adding the columnar sludge into the disc granulator, rotating for 1.5h, and enabling the granular sludge to be spherical.
(5) Preparing a mixed solution of 15mg/L ammonium molybdate and 10mg/L boric acid, immersing the granular sludge in the solution, stirring for 24 hours at 25 ℃, taking out, cleaning for 3 times by using tap water, and immersing for 12 hours to obtain the modified granular sludge.
(6) Setting the rotating speed of the disc granulator to 45r/min, setting the inclination angle to 55 degrees, putting the modified granular sludge into the disc granulator, starting to spray corn flour after rotating for 10min, continuing to roll to uniformly coat the corn flour on the surface of the granular sludge, stopping after running for 1h, and passing the sludge in the disc through a screen with the aperture of 0.5mm to obtain the pre-granulated granular sludge.
The water treatment effect of the granular sludge prepared in comparative example 3 was tested in the test manner of example 2, and as a result, it was found that the operation was performed for 10 daysAfter that, the granular sludge in the pre-granulation granular sludge AGS reaction tank of the comparative example 3 is added to have a good running state, the average grain diameter of the sludge is 1.3mm, and SVI is carried out 5 26mL/g SVI 30 24mL/g SVI 30 /SVI 5 At 0.92, the roughness was about 0.013Ra, with no significant change from the granular sludge of example 2. The COD of the effluent is 41mg/L, the total nitrogen is 8.58mg/L, the ammonia nitrogen is 0.7mg/L, the total phosphorus is 0.41mg/L, and the quality of the effluent reaches the first-level A standard in the 5 th day. After 40 days of operation, there was no significant change in total nitrogen in the effluent of the granular sludge of comparative example 3, as compared to the pre-granulated granular sludge of example 2, and the total phosphorus concentration was increased by about 17%, indicating a decrease in the phosphorus removal capacity of the granular sludge without the addition of candida arvensis. After 90 days of operation, the aerobic granular sludge can still keep stable structure.
Comparative example 4
The lactobacillus fermentum in step (2) was omitted and the rest was the same as in example 2.
(1) Taking 100L of dewatered sludge of town sewage plants with the water content of about 80 percent.
(2) Activating and enriching the candida utilis in a YPD culture medium, taking a saccharomycete aggregate in a logarithmic phase during activation, filtering the saccharomycete aggregate by using sterile gauze, and cleaning the saccharomycete aggregate by using sterile normal saline for 3 times to obtain saccharomycete suspension.
(3) 0.5L of bacterial suspension is added into 100L of dehydrated sludge, stirred for 30min to be fully mixed, and the mixture is subjected to static culture for 12h at 20 ℃.
(4) Respectively weighing 0.3kg of I-type carrageenan, 0.3kg of sodium alginate, 0.2kg of locust bean gum and 0.2kg of guar gum to compound into a binder, weighing 5kg of powdered activated carbon, adding 10g of 3, 4-dihydroxyphenethyl-acrylamide and 2g of dopamine hydrochloride into 20L of methanol, uniformly stirring, regulating the pH to 8-9 by using trihydroxy aminomethane, completely immersing the powdered activated carbon in the solution, oscillating for 12 hours at 25 ℃, then taking out the activated carbon and flushing with deionized water for 3 times to obtain the powdered activated carbon with double bond function. Immersing double bond functional powder active carbon in a mixed aqueous solution of sodium p-vinyl sulfonate (SPS) with the mass fraction of 10% and Ammonium Persulfate (APS) with the mass fraction of 0.1%, carrying out oscillation soaking for 0.5h, then polymerizing at 80 ℃ for 12h, taking out the active carbon, washing with deionized water for 3 times, carrying out oscillation soaking for 0.5h, and washing for 3 times to obtain the mussel bionic powder active carbon.
(5) Adding the compound binder and the mussel bionic powder active carbon into the dehydrated sludge, and uniformly stirring for 3 hours to fully mix the dehydrated sludge. And extruding the obtained sludge to pass through a screen with the aperture of 1.3mm, sieving and cutting the sludge at a constant speed to obtain cylindrical sludge with the bottom surface diameter of 1.3mm and the height of 1.3 mm. Setting the rotating speed of the disc granulator to 45r/min, setting the inclination angle to 55 degrees, adding the columnar sludge into the disc granulator, rotating for 1.5h, and enabling the granular sludge to be spherical.
(6) Preparing a mixed solution of 15mg/L ammonium molybdate and 10mg/L boric acid, immersing the granular sludge in the solution, stirring for 24 hours at 25 ℃, taking out, cleaning for 3 times by using tap water, and immersing for 12 hours to obtain the modified granular sludge.
(7) Setting the rotating speed of the disc granulator to 45r/min, setting the inclination angle to 55 degrees, putting the modified granular sludge into the disc granulator, starting to spray corn flour after rotating for 10min, continuing to roll to uniformly coat the corn flour on the surface of the granular sludge, stopping after running for 1h, and passing the sludge in the disc through a screen with the aperture of 0.5mm to obtain the pre-granulated granular sludge.
The water treatment effect of the granular sludge prepared in comparative example 4 was tested according to the test method of example 2, and found that the granular sludge in the AGS reaction tank for pre-granulated granular sludge added in comparative example 4 had a good running state after 10 days of running, the average particle size of the sludge was 1.2mm, and SVI 5 26mL/g SVI 30 24mL/g SVI 30 /SVI 5 0.92 SVI 30 Compared with the granular sludge in the example 2, the roughness is about 0.0146Ra, and the roughness is improved by 12% compared with the example 2. The COD of the effluent is 40mg/L, the total nitrogen is 8.84mg/L, the ammonia nitrogen is 0.73mg/L, the total phosphorus is 0.37mg/L, and the quality of the effluent reaches the first-level A standard in the 4d stage. After 40 days of operation, compared with the pre-granulated granular sludge of example 2, the effluent quality of the granular sludge of comparative example 4 is not changed obviously, the particle size is reduced by 16%, and after 90 days of operation, the aerobic granular sludge is crushed in small quantity.
Comparative example 5
The procedure of example 2 was repeated except that the mixed solution of ammonium molybdate and boric acid in step (6) was modified to a mixed solution of potassium chloride and sodium sulfate.
(1) Taking 100L of dewatered sludge of town sewage plants with the water content of about 80 percent.
(2) Activating and enriching the candida utilis in a YPD culture medium, taking a saccharomycete aggregate in a logarithmic phase during activation, filtering the saccharomycete aggregate by using sterile gauze, and cleaning the saccharomycete aggregate by using sterile normal saline for 3 times to obtain saccharomycete suspension. Lactobacillus fermentum was purchased from Shanghai, highway Biotechnology, inc., inoculated into sterilized MRS medium, and subjected to activation culture at 37deg.C for three generations, each generation being cultured for 24 hr, and the fermentation broth grown from the third generation to logarithmic phase was used for inoculation.
(3) 0.5L of bacterial suspension and 0.5L of fermentation liquor are added into 100L of dehydrated sludge, stirred for 30min to be fully mixed, and the mixture is subjected to static culture for 12h at 20 ℃.
(4) Respectively weighing 0.3kg of I-type carrageenan, 0.3kg of sodium alginate, 0.2kg of locust bean gum and 0.2kg of guar gum to compound into a binder, weighing 5kg of powdered activated carbon, adding 10g of 3, 4-dihydroxyphenethyl-acrylamide and 2g of dopamine hydrochloride into 20L of methanol, uniformly stirring, regulating the pH to 8-9 by using trihydroxy aminomethane, completely immersing the powdered activated carbon in the solution, oscillating for 12 hours at 25 ℃, then taking out the activated carbon and flushing with deionized water for 3 times to obtain the powdered activated carbon with double bond function. Immersing double bond functional powder active carbon in a mixed aqueous solution of sodium p-vinyl sulfonate (SPS) with the mass fraction of 10% and Ammonium Persulfate (APS) with the mass fraction of 0.1%, carrying out oscillation soaking for 0.5h, then polymerizing at 80 ℃ for 12h, taking out the active carbon, washing with deionized water for 3 times, carrying out oscillation soaking for 0.5h, and washing for 3 times to obtain the mussel bionic powder active carbon.
(5) Adding the compound binder and the mussel bionic powder active carbon into the dehydrated sludge, and uniformly stirring for 3 hours to fully mix the dehydrated sludge. And extruding the obtained sludge to pass through a screen with the aperture of 1.3mm, sieving and cutting the sludge at a constant speed to obtain cylindrical sludge with the bottom surface diameter of 1.3mm and the height of 1.3 mm. Setting the rotating speed of the disc granulator to 45r/min, setting the inclination angle to 55 degrees, adding the columnar sludge into the disc granulator, rotating for 1.5h, and enabling the granular sludge to be spherical.
(6) Preparing a mixed solution of 15mg/L potassium chloride and 10mg/L sodium sulfate, immersing the granular sludge in the solution, stirring for 24 hours at 25 ℃, taking out, cleaning for 3 times by using tap water, and immersing for 12 hours to obtain the modified granular sludge.
(7) Setting the rotating speed of the disc granulator to 45r/min, setting the inclination angle to 55 degrees, putting the modified granular sludge into the disc granulator, starting to spray corn flour after rotating for 10min, continuing to roll to uniformly coat the corn flour on the surface of the granular sludge, stopping after running for 1h, and passing the sludge in the disc through a screen with the aperture of 0.5mm to obtain the pre-granulated granular sludge.
The water treatment effect of the granular sludge prepared in comparative example 5 was tested according to the test method of example 2, and as a result, it was found that the granular sludge in the AGS reaction tank for pre-granulated granular sludge added in comparative example 5 was operated in a good state after 10 days of operation, the average particle size of the sludge was 1.15mm, and SVI 5 26mL/g SVI 30 24mL/g SVI 30 /SVI 5 0.92 SVI 30 Compared with the granular sludge in the example 2, the roughness is about 0.0153Ra, and the roughness is improved by 18% compared with the example 2. The COD of the effluent is 40mg/L, the total nitrogen is 8.84mg/L, the ammonia nitrogen is 0.73mg/L, the total phosphorus is 0.42mg/L, and the quality of the effluent reaches the first-level A standard in the 5 th day. After 40 days of operation, the total phosphorus concentration of the granular sludge effluent of comparative example 5 was increased by 20% compared with that of the pre-granulated granular sludge of example 2, the particle size was reduced by 19.6%, and after 90 days of operation the aerobic granular sludge was crushed in small amounts.
Comparative example 6
The corn meal of step (7) was omitted and the remainder was the same as in example 2.
(1) Taking 100L of dewatered sludge of town sewage plants with the water content of about 80 percent.
(2) Activating and enriching the candida utilis in a YPD culture medium, taking a saccharomycete aggregate in a logarithmic phase during activation, filtering the saccharomycete aggregate by using sterile gauze, and cleaning the saccharomycete aggregate by using sterile normal saline for 3 times to obtain saccharomycete suspension. Lactobacillus fermentum was purchased from Shanghai, highway Biotechnology, inc., inoculated into sterilized MRS medium, and subjected to activation culture at 37deg.C for three generations, each generation being cultured for 24 hr, and the fermentation broth grown from the third generation to logarithmic phase was used for inoculation.
(3) 0.5L of bacterial suspension and 0.5L of fermentation liquor are added into 100L of dehydrated sludge, stirred for 30min to be fully mixed, and the mixture is subjected to static culture for 12h at 20 ℃.
(4) Respectively weighing 0.3kg of I-type carrageenan, 0.3kg of sodium alginate, 0.2kg of locust bean gum and 0.2kg of guar gum to compound into a binder, weighing 5kg of powdered activated carbon, adding 10g of 3, 4-dihydroxyphenethyl-acrylamide and 2g of dopamine hydrochloride into 20L of methanol, uniformly stirring, regulating the pH to 8-9 by using trihydroxy aminomethane, completely immersing the powdered activated carbon in the solution, oscillating for 12 hours at 25 ℃, then taking out the activated carbon and flushing with deionized water for 3 times to obtain the powdered activated carbon with double bond function. Immersing double bond functional powder active carbon in a mixed aqueous solution of sodium p-vinyl sulfonate (SPS) with the mass fraction of 10% and Ammonium Persulfate (APS) with the mass fraction of 0.1%, carrying out oscillation soaking for 0.5h, then polymerizing at 80 ℃ for 12h, taking out the active carbon, washing with deionized water for 3 times, carrying out oscillation soaking for 0.5h, and washing for 3 times to obtain the mussel bionic powder active carbon.
(5) Adding the compound binder and the mussel bionic powder active carbon into the dehydrated sludge, and uniformly stirring for 3 hours to fully mix the dehydrated sludge. And extruding the obtained sludge to pass through a screen with the aperture of 1.3mm, sieving and cutting the sludge at a constant speed to obtain cylindrical sludge with the bottom surface diameter of 1.3mm and the height of 1.3 mm. Setting the rotating speed of the disc granulator to 45r/min, setting the inclination angle to 55 degrees, adding the columnar sludge into the disc granulator, rotating for 1.5h, and enabling the granular sludge to be spherical.
(6) Preparing a mixed solution of 15mg/L ammonium molybdate and 10mg/L boric acid, immersing the granular sludge in the solution, stirring for 24 hours at 25 ℃, taking out, cleaning for 3 times by using tap water, and immersing for 12 hours to obtain the modified granular sludge.
(7) Setting the rotating speed of the disc granulator to 45r/min, setting the inclination angle to 55 degrees, putting the modified granular sludge into the disc granulator, stopping after rotating for 1h, and enabling the sludge in the disc to pass through a screen with the aperture of 0.5mm to obtain the pre-granulated granular sludge.
The water treatment effect of the granular sludge prepared in comparative example 6 was tested according to the test method of example 2, and as a result, it was found that the granular sludge in the AGS reaction tank for pre-granulated granular sludge added in comparative example 6 was operated in a good state after 10 days of operation, the average particle size of the sludge was 1.37mm, and SVI 5 25mL/g SVI 30 23mL/g SVI 30 /SVI 5 At the level of 0.92 of the total weight of the product,the roughness was about 0.0135Ra, with no significant change in the granular sludge compared to example 2. The COD of the effluent is 43mg/L, the total nitrogen is 9.23mg/L, the ammonia nitrogen is 0.93mg/L, the total phosphorus is 0.35mg/L, and the quality of the effluent reaches the first grade A standard in the 4 d. After 40 days of operation, compared with the pre-granulated granular sludge of the embodiment 2, the ammonia nitrogen concentration of effluent water of the granular sludge of the comparative example 6 is improved by 16%, and after 90 days of operation, the aerobic granular sludge can still keep stable structure.
Comparative example 7
The procedure of example 2 was followed except that the compound binder in step (4) was changed to a single hydrocolloid sodium alginate.
(1) Taking 100L of dewatered sludge of town sewage plants with the water content of about 80 percent.
(2) Activating and enriching the candida utilis in a YPD culture medium, taking a saccharomycete aggregate in a logarithmic phase during activation, filtering the saccharomycete aggregate by using sterile gauze, and cleaning the saccharomycete aggregate by using sterile normal saline for 3 times to obtain saccharomycete suspension. Lactobacillus fermentum was purchased from Shanghai, highway Biotechnology, inc., inoculated into sterilized MRS medium, and subjected to activation culture at 37deg.C for three generations, each generation being cultured for 24 hr, and the fermentation broth grown from the third generation to logarithmic phase was used for inoculation.
(3) 0.5L of bacterial suspension and 0.5L of fermentation liquor are added into 100L of dehydrated sludge, stirred for 30min to be fully mixed, and the mixture is subjected to static culture for 12h at 20 ℃.
(4) Weighing 1.0kg of sodium alginate as a binder; weighing 5kg of powdered activated carbon, adding 10g of 3, 4-dihydroxyphenethyl-acrylamide and 2g of dopamine hydrochloride into 20L of methanol, uniformly stirring, regulating the pH to 8-9 by using trihydroxy aminomethane, completely immersing the powdered activated carbon in the solution, oscillating for 12 hours at 25 ℃, then taking out the activated carbon, and washing 3 times by using deionized water to obtain the powdered activated carbon with double bond function. Immersing double bond functional powder active carbon in a mixed aqueous solution of sodium p-vinyl sulfonate (SPS) with the mass fraction of 10% and Ammonium Persulfate (APS) with the mass fraction of 0.1%, carrying out oscillation soaking for 0.5h, then polymerizing at 80 ℃ for 12 h, taking out the active carbon, washing with deionized water for 3 times, carrying out oscillation soaking for 0.5h, and washing for 3 times to obtain the mussel bionic powder active carbon.
(5) Adding the binder and the mussel bionic powder active carbon into the dehydrated sludge, and uniformly stirring for 3 hours to fully mix the materials. And extruding the obtained sludge to pass through a screen with the aperture of 1.3mm, sieving and cutting the sludge at a constant speed to obtain cylindrical sludge with the bottom surface diameter of 1.3mm and the height of 1.3 mm. Setting the rotating speed of the disc granulator to 45r/min, setting the inclination angle to 55 degrees, adding the columnar sludge into the disc granulator, rotating for 1.5h, and enabling the granular sludge to be spherical.
(6) Preparing a mixed solution of 15mg/L ammonium molybdate and 10mg/L boric acid, immersing the granular sludge in the solution, stirring for 24 hours at 25 ℃, taking out, cleaning for 3 times by using tap water, and immersing for 12 hours to obtain the modified granular sludge.
(7) Setting the rotating speed of the disc granulator to 45r/min, setting the inclination angle to 55 degrees, putting the modified granular sludge into the disc granulator, starting to spray corn flour after rotating for 10min, continuing to roll to uniformly coat the corn flour on the surface of the granular sludge, stopping after running for 1h, and passing the sludge in the disc through a screen with the aperture of 0.5mm to obtain the pre-granulated granular sludge.
The water treatment effect of the granular sludge prepared in comparative example 7 was tested according to the test method of example 2, and as a result, it was found that the granular sludge in the AGS reaction tank for pre-granulated granular sludge added in comparative example 7 was operated for 10 days in a good state, the average particle size of the sludge was 1.17mm, and SVI was obtained 5 30mL/g SVI 30 27mL/g SVI 30 /SVI 5 0.9 SVI 30 The granular sludge of example 2 was not significantly changed, and the roughness was about 0.0142Ra, 9% higher than that of example 2. The COD of the effluent is 42mg/L, the total nitrogen is 9.16mg/L, the ammonia nitrogen is 0.85mg/L, the total phosphorus is 0.35mg/L, and the quality of the effluent reaches the first-level A standard in the 5 th day. After 40 days of operation, compared with the pre-granulated granular sludge of example 2, the effluent quality of the granular sludge of comparative example 7 is not changed significantly, the particle size is reduced by 18%, and after 90 days of operation, the aerobic granular sludge is crushed in small quantity.
Comparative example 8
The procedure of example 2 was repeated except that the steps (3), (5) and (6) were changed.
(1) Taking 100L of dewatered sludge of town sewage plants with the water content of about 80 percent.
(2) Activating and enriching the candida utilis in a YPD culture medium, taking a saccharomycete aggregate in a logarithmic phase during activation, filtering the saccharomycete aggregate by using sterile gauze, and cleaning the saccharomycete aggregate by using sterile normal saline for 3 times to obtain saccharomycete suspension. Lactobacillus fermentum was purchased from Shanghai, highway Biotechnology, inc., inoculated into sterilized MRS medium, and subjected to activation culture at 37deg.C for three generations, each generation being cultured for 24 hr, and the fermentation broth grown from the third generation to logarithmic phase was used for inoculation.
(3) Respectively weighing 0.3kg of I-type carrageenan, 0.3kg of sodium alginate, 0.2kg of locust bean gum and 0.2kg of guar gum to compound into a binder, weighing 5kg of powdered activated carbon, adding 10g of 3, 4-dihydroxyphenethyl-acrylamide and 2g of dopamine hydrochloride into 20L of methanol, uniformly stirring, regulating the pH to 8-9 by using trihydroxy aminomethane, completely immersing the powdered activated carbon in the solution, oscillating for 12 hours at 25 ℃, then taking out the activated carbon and flushing with deionized water for 3 times to obtain the powdered activated carbon with double bond function. Immersing double bond functional powder active carbon in a mixed aqueous solution of sodium p-vinyl sulfonate (SPS) with the mass fraction of 10% and Ammonium Persulfate (APS) with the mass fraction of 0.1%, carrying out oscillation soaking for 0.5h, then polymerizing at 80 ℃ for 12 h, taking out the active carbon, washing with deionized water for 3 times, carrying out oscillation soaking for 0.5h, and washing for 3 times to obtain the mussel bionic powder active carbon.
(4) Adding the binder and the mussel bionic powder active carbon into the dehydrated sludge, and uniformly stirring for 3 hours to fully mix the materials.
(5) Preparing a mixed solution of 15mg/L ammonium molybdate and 10mg/L boric acid, immersing the dehydrated sludge in the solution, stirring for 24 hours at 25 ℃, taking out, cleaning for 3 times by using tap water, and immersing for 12 hours to obtain the modified sludge.
(6) Adding 0.5L of bacterial suspension and 0.5L of fermentation liquor into the modified sludge, stirring for 30min to fully mix, and standing and culturing for 12h at 20 ℃.
(7) And extruding the obtained sludge to pass through a screen with the aperture of 1.3mm, sieving and cutting the sludge at a constant speed to obtain cylindrical sludge with the bottom surface diameter of 1.3mm and the height of 1.3 mm. Setting the rotating speed of the disc granulator to 45r/min, setting the inclination angle to 55 degrees, adding the columnar sludge into the disc granulator, rotating for 1.5h, and enabling the granular sludge to be spherical.
(8) Setting the rotating speed of the disc granulator to 45r/min, setting the inclination angle to 55 degrees, putting the modified granular sludge into the disc granulator, starting to spray corn flour after rotating for 10min, continuing to roll to uniformly coat the corn flour on the surface of the granular sludge, stopping after running for 1h, and passing the sludge in the disc through a screen with the aperture of 0.5mm to obtain the pre-granulated granular sludge.
The water treatment effect of the granular sludge prepared in comparative example 8 was tested according to the test method of example 2, and as a result, it was found that the granular sludge in the AGS reaction tank for pre-granulated granular sludge added in comparative example 8 was operated in a good state after 10 days of operation, the average particle size of the sludge was 1.25mm, and SVI 5 28mL/g SVI 30 26.3mL/g SVI 30 /SVI 5 At 0.94, the roughness was about 0.0138Ra, with no significant change in the granular sludge compared to example 2. The COD of the effluent is 42mg/L, the total nitrogen is 9.49mg/L, the ammonia nitrogen is 1.32mg/L, the total phosphorus is 0.41mg/L, and the quality of the effluent reaches the first-level A standard in the 5 th day. After 40 days of operation, the total nitrogen concentration of the effluent of the granular sludge of comparative example 8 was increased by 6% and the total phosphorus concentration of the effluent was increased by 17% compared with the pre-granulated granular sludge of example 2, the particle size of the granules was reduced by 12.6%, and after 90 days of operation the aerobic granular sludge was crushed in small amounts.
Claims (7)
1. A rapid generation method of granular sludge suitable for town sewage treatment, which is characterized by comprising the following steps:
(1) Taking dewatered sludge of town sewage plants as a basic material;
(2) Adding candida utilis and lactobacillus fermentum into the dehydrated sludge, uniformly stirring, and then standing, culturing and fermenting;
(3) After fermentation, adding a compound binder and mussel bionic powder active carbon, uniformly stirring, and cutting by a screen and rolling by a disc to form granular sludge;
(4) Immersing the granular sludge obtained in the step (3) in a modified solution of ammonium molybdate and boric acid, uniformly stirring, and repeatedly cleaning with water to obtain modified granular sludge;
(5) Adding the modified granular sludge into corn flour, rolling to uniformly coat the surface of the granular sludge, and sieving to obtain pre-granulated aerobic granular sludge;
the mussel bionic powder activated carbon is prepared by introducing double bond functional groups on the surface of the powder activated carbon through a mussel bionic technology, and then modifying sulfonic groups on the surface of the activated carbon through an in-situ polymerization method; specifically, the mussel bionic powder activated carbon is prepared by the following method: adding a proper amount of 3, 4-dihydroxyphenethyl-acrylamide and dopamine hydrochloride into methanol, uniformly stirring, regulating the pH to 8-9 by using trihydroxy aminomethane, completely immersing the powder active carbon in the solution, oscillating for 12-24 hours at 25-30 ℃, then taking out the active carbon and flushing with deionized water for 3 times to obtain the powder active carbon with double bond function, immersing the double bond functional powder active carbon in an aqueous solution of sodium p-styrenesulfonate and ammonium persulfate, oscillating and immersing for 0.5-3 hours, polymerizing for 12-18 hours at 80-100 ℃, taking out the active carbon, cleaning for several times by using deionized water, oscillating and immersing for 0.5-3 hours, and cleaning for several times to obtain the mussel bionic powder active carbon;
in the step (3), the compound binder is selected from the mixture of more than two of I-carrageenan, alginate, locust bean gum and guar gum, and the addition amount is 1-25% of the mass of the dehydrated sludge;
In the step (3), the adding amount of the mussel bionic powder active carbon is 1-20% by volume of dehydrated sludge, and the stirring time is 0.1-20h; in the step (4), the concentration of the ammonium molybdate solution is 15-50 mg/L, the concentration of the boric acid solution is 10-40 mg/L, and the mixture is stirred for 10-30h at 20-35 ℃.
2. The method according to claim 1, wherein in the step (1), the water content of the dewatered sludge of the town sewage plant is 65% -85%.
3. The method according to claim 1, wherein in the step (2), the amount of the Candida arvensis added is 0.5% to 10%, and the OD of the yeasts in the bacterial suspension is the same 600 The value is 0.4-2.3.
4. According to claim 1The method for producing the bacterial strain according to any one of the preceding claims 3, wherein in the step (2), the amount of the lactobacillus fermentum added is 0.5 to 10%, and the OD of the lactobacillus fermentum in the bacterial suspension is the same as that of the lactobacillus fermentum 600 The value is 0.6-3.6.
5. The granular sludge suitable for town sewage treatment obtained by the production method according to any one of claims 1 to 4.
6. A method for treating sewage, characterized in that the method uses the granular sludge suitable for town sewage treatment as claimed in claim 5 for treatment.
7. The use of the granular sludge for town sewage treatment as claimed in claim 5 in the environmental area.
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