CN110387368A - A kind of formula and preparation method thereof of landscape water body remediation microbial inoculum - Google Patents
A kind of formula and preparation method thereof of landscape water body remediation microbial inoculum Download PDFInfo
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- CN110387368A CN110387368A CN201910686590.0A CN201910686590A CN110387368A CN 110387368 A CN110387368 A CN 110387368A CN 201910686590 A CN201910686590 A CN 201910686590A CN 110387368 A CN110387368 A CN 110387368A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
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- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
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Abstract
The invention discloses a kind of formulas and preparation method thereof of landscape water body remediation microbial inoculum, more particularly to treating landscape water body technical field, including carrier, the major ingredient that the carrier uses includes: 10-30 parts of wheat bran, 6-10 parts of zein and 40-60 parts of diatomite by weight, further includes 8-10 parts of compound bacteria.The present invention in water body by adding composite bacteria agent, and provide carrier, live and reproduce composite bacteria agent easily in water environment, form beneficial advantage group, regulate and control microbial ecological structure in water body, while effective bacterium degrades and converts harmful substance in water in microbial inoculum, promote the growth of indigenous flora, inhibit the excessive multiplication of algae, prevents and treats aquatic animal disease, improve the survival rate of effective bacterium in water, preferably keep activity, harmful substance is adsorbed, has the function that the purpose of efficient degradation plays and purifies water, achievees the effect that restoring landscape water body ecology.
Description
Technical field
The present invention relates to treating landscape water body technical fields, it is more particularly related to a kind of landscape water body reparation
Formula of microbial inoculum and preparation method thereof.
Background technique
China is in urbanization process at present, urban construction, old town transformation and plot planning in recent years
In, park, the residential quarter being the theme with landscape water emerge in multitude.Landscape water body refer to naturally occur or manually build, to people
With the water body of the city of aesthetic feeling, rural area and tourist attractions.Most landscape water body belongs to closing, semi-closed state, water body sheet
Poor fluidity, the flow of body are smaller.These factors cause the detergent power of landscape water itself and environmental carrying capacity extremely limited, when interior
When source contact scar or the influence of exogenous pollution are more than the environmental carrying capacity of water body, it is easy to that water pollution is caused to deteriorate.In
Under the action of pollution, when the conditions such as temperature, illumination are suitable for, the mass propagations such as Measures of Algae in Water Body, microorganism cause water body to lack
Oxygen, transparency reduce, and water body generates peculiar smell, and aquatic animal is dead, cause the vicious circle state for being difficult to restore.
Currently, artificial intervention pollution administration water body is highly efficient, specific method has: being oxygenated, launches in serike, water
All kinds of medicaments etc..Usually there is treatment cost height, produces effects undesirable, cause in pollution administration water body in common traditional medicament
The problem of secondary pollution etc..Administering water body using microorganism formulation is the new technology risen in recent years, mainly in polluted-water
In artificially add microorganism, or the method by manually cultivating indigenous microorganism in water, utilize microorganism absorb, conversion or drop
Water pollutant is solved, elimination pollution, purifying water body, the biological control measure for restoring Ecology function are reached, the advantage of the method is
Cost of investment is lower, and effect is good, does not easily cause pollution administration.Existing microbial inoculum Shortcomings during pollution administration water body,
On the one hand, it needs to optimize various floras proportion in composite bacteria agent, is acted synergistically between each flora with improving, on the other hand, need to adjust
Carrier ratio reduces flora and survives in water environment the influence of resistance, improves effective bacterium survival rate.
Therefore, inventing formula of a kind of landscape water body remediation microbial inoculum and preparation method thereof, have very much must to solve the above problems
It wants.
Summary of the invention
In order to overcome the drawbacks described above of the prior art, the embodiment of the present invention provides a kind of matching for landscape water body remediation microbial inoculum
Side and preparation method thereof, by adding composite bacteria agent in water body, and provides carrier, makes composite bacteria agent Yi Shengcun in water environment
Breeding forms beneficial advantage group, regulates and controls microbial ecological structure in water body, has in effective bacterium degradation and conversion water in microbial inoculum
While evil substance, promote the growth of indigenous flora, inhibit the excessive multiplication of algae, prevent and treat aquatic animal disease, improves effective
The survival rate of bacterium in water preferably keeps activity, adsorbs harmful substance, achievees the purpose that efficient degradation plays and purifies water
Effect achievees the effect that restoring landscape water body ecology, to solve the problems, such as proposed in background technique.
To achieve the above object, the invention provides the following technical scheme: a kind of formula of landscape water body remediation microbial inoculum, including
Carrier, the major ingredient that the carrier uses include: 10-30 parts of wheat bran, 6-10 parts of zein and diatomite 40-60 by weight
Part, it further include 8-10 parts of compound bacteria.
Preferably, the major ingredient that the carrier uses includes: wheat bran 3-10kg, zein 2-3kg and diatomite by weight
6-20kg further includes compound bacteria 1-4kg.
Preferably, the compound bacteria by Rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, fibrosis Nocard's bacillus,
Lactobacillus plantarum and bacillus subtilis composition, the Rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, fibrosis promise
The mixing mass ratio of Cattell bacterium, lactobacillus plantarum and bacillus subtilis is arranged are as follows: 2-4:2-4:1-3:2-6:4-6:1-4.
Preferably, the Rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, fibrosis Nocard's bacillus, plant cream bar
Bacterium and bacillus subtilis are used to prepare bacteria suspension.
Preferably, the viable count of a variety of bacteria suspensions is disposed as 1.0 × 108-1.0×109cfu/mL。
A kind of preparation method of landscape water body remediation microbial inoculum, concrete operation step are as follows:
Step 1: culture bacterium is taken from the bacterium colony in culture medium with oese, is placed in the test tube equipped with sterile water, with strength
Mix, prepare bacteria suspension, and in proportion by Rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, fibrosis Nocard's bacillus,
Bacteria suspension is respectively prepared in lactobacillus plantarum and bacillus subtilis；
Step 2: a variety of bacteria suspensions made of step 1 are poured into graduated cylinder, and are uniformly mixed, compound bacteria is obtained；
Step 3: taking wheat bran, zein and diatomite spare in proportion, and the wheat bran, zein and diatomite of preparation are stirred
Mixing is mixed, powder carrier is obtained；
Step 4: the composite bacteria liquid that step 2 obtains is admixed and is uniformly mixed with the powder carrier that step 3 obtains, i.e.,
Water remediation microbial inoculum semi-finished product can be obtained；
Step 5: stabilizer is added in the water remediation microbial inoculum semi-finished product prepared in step 4, again after mixing
Obtain water remediation microbial inoculum finished product.
Preferably, stabilizer is set as one of potassium sorbate or sodium benzoate, the stabilizer in the step 5
1:55 is set as with the adding proportion of water remediation microbial inoculum semi-finished product.
Preferably, further include the application method of landscape water body remediation microbial inoculum, specifically used step are as follows: water remediation microbial inoculum at
Product are activating 4-6h using molasses water using preceding at room temperature.
Technical effect and advantage of the invention:
1, by adding composite bacteria agent in water body, and carrier is provided, lives and reproduces composite bacteria agent easily in water environment,
Beneficial advantage group is formed, microbial ecological structure in water body is regulated and controled, nuisance in water is degraded and converted to effectively bacterium in microbial inoculum
While matter, promote the growth of indigenous flora, inhibit the excessive multiplication of algae, prevents and treats aquatic animal disease, improve effective bacterium and exist
Survival rate in water preferably keeps activity, adsorbs harmful substance, achievees the purpose that efficient degradation plays the work to purify water
With, achieve the effect that restoring landscape water body ecology；
2, it is limited by compound bacteria in water remediation microbial inoculum and the proportionate relationship of carrier, maintains water remediation bacterium
The balanced relation of compound bacteria and microbe carrier in agent, comprehensively consider interact between the special role and flora of each flora it is same
When, so that each flora is maintained relatively steady state, improves water body purification effect；
3, nontoxic by the way that diatomite ingredient is arranged in the carrier, have that voidage is big, absorbability is strong and chemical property
Stable feature, effectively absorption microbial cells, provide good living environment for it, improve the survival rate of microorganism, absorption collection
Middle harmful substance improves the efficiency of microbial degradation conversion harmful substance, also added stabilizer in water remediation microbial inoculum, just
In the marketing of water remediation microbial inoculum.
Specific embodiment
It is clearly and completely described below in conjunction with the technical solution in the embodiment of the present invention, it is clear that described reality
Applying example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is general
Logical technical staff every other embodiment obtained without making creative work belongs to what the present invention protected
Range.
Embodiment 1:
The present invention provides a kind of formula of landscape water body remediation microbial inoculum, including carrier, the major ingredient that the carrier uses is pressed
Poidometer includes: wheat bran 5kg, zein 3kg and diatomite 20kg, further includes compound bacteria 4kg；
Further, the compound bacteria is by Rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, fibrosis promise Cattell
Bacterium, lactobacillus plantarum and bacillus subtilis composition, the Rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, fibrosis
Nocard's bacillus, lactobacillus plantarum and the setting of the mixing mass ratio of bacillus subtilis are as follows: 2:2:1:2:4:1；
Further, the Rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, fibrosis Nocard's bacillus, plant cream
Bacillus and bacillus subtilis are used to prepare bacteria suspension；
Further, the viable count of a variety of bacteria suspensions is disposed as 1.0 × 108cfu/mL。
A kind of preparation method of landscape water body remediation microbial inoculum, concrete operation step are as follows:
Step 1: culture bacterium is taken from the bacterium colony in culture medium with oese, is placed in the test tube equipped with sterile water, with strength
Mix, prepare bacteria suspension, and in proportion by Rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, fibrosis Nocard's bacillus,
Bacteria suspension is respectively prepared in lactobacillus plantarum and bacillus subtilis；
Step 2: a variety of bacteria suspensions made of step 1 are poured into graduated cylinder, and are uniformly mixed, compound bacteria is obtained；
Step 3: taking wheat bran, zein and diatomite spare in proportion, and the wheat bran, zein and diatomite of preparation are stirred
Mixing is mixed, powder carrier is obtained；
Step 4: the composite bacteria liquid that step 2 obtains is admixed and is uniformly mixed with the powder carrier that step 3 obtains, i.e.,
Water remediation microbial inoculum semi-finished product can be obtained；
Step 5: stabilizer is added in the water remediation microbial inoculum semi-finished product prepared in step 4, stabilizer is set as benzene
Sodium formate, and the dosage of stabilizer is set as 0.01kg, obtains water remediation microbial inoculum finished product after mixing again.
It further include the application method of landscape water body remediation microbial inoculum, specifically used step are as follows: water remediation microbial inoculum finished product makes
4h is activated at room temperature using molasses water with preceding；
Embodiment 2:
The present invention provides a kind of formula of landscape water body remediation microbial inoculum, including carrier, the major ingredient that the carrier uses is pressed
Poidometer includes: wheat bran 3kg, zein 1kg and diatomite 6kg, further includes compound bacteria 1kg；
Further, the compound bacteria is by Rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, fibrosis promise Cattell
Bacterium, lactobacillus plantarum and bacillus subtilis composition, the Rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, fibrosis
Nocard's bacillus, lactobacillus plantarum and the setting of the mixing mass ratio of bacillus subtilis are as follows: 2:2:1:2:4:1；
Further, the Rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, fibrosis Nocard's bacillus, plant cream
Bacillus and bacillus subtilis are used to prepare bacteria suspension；
Further, the viable count of a variety of bacteria suspensions is disposed as 1.0 × 109cfu/mL。
A kind of preparation method of landscape water body remediation microbial inoculum, concrete operation step are as follows:
Step 1: culture bacterium is taken from the bacterium colony in culture medium with oese, is placed in the test tube equipped with sterile water, with strength
Mix, prepare bacteria suspension, and in proportion by Rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, fibrosis Nocard's bacillus,
Bacteria suspension is respectively prepared in lactobacillus plantarum and bacillus subtilis；
Step 2: a variety of bacteria suspensions made of step 1 are poured into graduated cylinder, and are uniformly mixed, compound bacteria is obtained；
Step 3: taking wheat bran, zein and diatomite spare in proportion, and the wheat bran, zein and diatomite of preparation are stirred
Mixing is mixed, powder carrier is obtained；
Step 4: the composite bacteria liquid that step 2 obtains is admixed and is uniformly mixed with the powder carrier that step 3 obtains, i.e.,
Water remediation microbial inoculum semi-finished product can be obtained；
Step 5: stabilizer is added in the water remediation microbial inoculum semi-finished product prepared in step 4, stabilizer is set as mountain
Potassium sorbate, and the dosage of stabilizer is set as 0.01kg, obtains water remediation microbial inoculum finished product after mixing again.
It further include the application method of landscape water body remediation microbial inoculum, specifically used step are as follows: water remediation microbial inoculum finished product makes
5h is activated at room temperature using molasses water with preceding.
Embodiment 3:
The present invention provides a kind of formula of landscape water body remediation microbial inoculum, including carrier, the major ingredient that the carrier uses is pressed
Poidometer includes: wheat bran 5kg, zein 3kg and diatomite 20kg, further includes compound bacteria 4kg；
Further, the compound bacteria is by Rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, fibrosis promise Cattell
Bacterium, lactobacillus plantarum and bacillus subtilis composition, the Rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, fibrosis
Nocard's bacillus, lactobacillus plantarum and the setting of the mixing mass ratio of bacillus subtilis are as follows: 4:4:3:6:6:4；
Further, the Rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, fibrosis Nocard's bacillus, plant cream
Bacillus and bacillus subtilis are used to prepare bacteria suspension；
Further, the viable count of a variety of bacteria suspensions is disposed as 1.0 × 109cfu/mL。
A kind of preparation method of landscape water body remediation microbial inoculum, concrete operation step are as follows:
Step 1: culture bacterium is taken from the bacterium colony in culture medium with oese, is placed in the test tube equipped with sterile water, with strength
Mix, prepare bacteria suspension, and in proportion by Rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, fibrosis Nocard's bacillus,
Bacteria suspension is respectively prepared in lactobacillus plantarum and bacillus subtilis；
Step 2: a variety of bacteria suspensions made of step 1 are poured into graduated cylinder, and are uniformly mixed, compound bacteria is obtained；
Step 3: taking wheat bran, zein and diatomite spare in proportion, and the wheat bran, zein and diatomite of preparation are stirred
Mixing is mixed, powder carrier is obtained；
Step 4: the composite bacteria liquid that step 2 obtains is admixed and is uniformly mixed with the powder carrier that step 3 obtains, i.e.,
Water remediation microbial inoculum semi-finished product can be obtained；
Step 5: stabilizer is added in the water remediation microbial inoculum semi-finished product prepared in step 4, stabilizer is set as benzene
Sodium formate, and the dosage of stabilizer is set as 0.05kg, obtains water remediation microbial inoculum finished product after mixing again.
It further include the application method of landscape water body remediation microbial inoculum, specifically used step are as follows: water remediation microbial inoculum finished product makes
6h is activated at room temperature using molasses water with preceding.
Embodiment 4:
The present invention provides a kind of formula of landscape water body remediation microbial inoculum, including carrier, the major ingredient that the carrier uses is pressed
Poidometer includes: wheat bran 3kg, zein 1kg and diatomite 6kg, further includes compound bacteria 1kg；
Further, the compound bacteria is by Rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, fibrosis promise Cattell
Bacterium, lactobacillus plantarum and bacillus subtilis composition, the Rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, fibrosis
Nocard's bacillus, lactobacillus plantarum and the setting of the mixing mass ratio of bacillus subtilis are as follows: 4:4:3:6:6:4；
Further, the Rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, fibrosis Nocard's bacillus, plant cream
Bacillus and bacillus subtilis are used to prepare bacteria suspension；
Further, the viable count of a variety of bacteria suspensions is disposed as 1.0 × 109cfu/mL。
A kind of preparation method of landscape water body remediation microbial inoculum, concrete operation step are as follows:
Step 1: culture bacterium is taken from the bacterium colony in culture medium with oese, is placed in the test tube equipped with sterile water, with strength
Mix, prepare bacteria suspension, and in proportion by Rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, fibrosis Nocard's bacillus,
Bacteria suspension is respectively prepared in lactobacillus plantarum and bacillus subtilis；
Step 2: a variety of bacteria suspensions made of step 1 are poured into graduated cylinder, and are uniformly mixed, compound bacteria is obtained；
Step 3: taking wheat bran, zein and diatomite spare in proportion, and the wheat bran, zein and diatomite of preparation are stirred
Mixing is mixed, powder carrier is obtained；
Step 4: the composite bacteria liquid that step 2 obtains is admixed and is uniformly mixed with the powder carrier that step 3 obtains, i.e.,
Water remediation microbial inoculum semi-finished product can be obtained；
Step 5: stabilizer is added in the water remediation microbial inoculum semi-finished product prepared in step 4, stabilizer is set as mountain
Potassium sorbate, and the dosage of stabilizer is set as 0.05kg, obtains water remediation microbial inoculum finished product after mixing again.
It further include the application method of landscape water body remediation microbial inoculum, specifically used step are as follows: water remediation microbial inoculum finished product makes
6h is activated at room temperature using molasses water with preceding.
Embodiment 5:
The present invention provides a kind of formula of landscape water body remediation microbial inoculum, including carrier, the major ingredient that the carrier uses is pressed
Poidometer includes: wheat bran 3kg, zein 1kg and diatomite 6kg, further includes compound bacteria 1kg；
Further, the compound bacteria is by Rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, fibrosis promise Cattell
Bacterium, lactobacillus plantarum and bacillus subtilis composition, the Rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, fibrosis
Nocard's bacillus, lactobacillus plantarum and the setting of the mixing mass ratio of bacillus subtilis are as follows: 2:2:1:2:4:1；
Further, the Rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, fibrosis Nocard's bacillus, plant cream
Bacillus and bacillus subtilis are used to prepare bacteria suspension；
Further, the viable count of a variety of bacteria suspensions is disposed as 1.0 × 109cfu/mL。
A kind of preparation method of landscape water body remediation microbial inoculum, concrete operation step are as follows:
Step 1: culture bacterium is taken from the bacterium colony in culture medium with oese, is placed in the test tube equipped with sterile water, with strength
Mix, prepare bacteria suspension, and in proportion by Rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, fibrosis Nocard's bacillus,
Bacteria suspension is respectively prepared in lactobacillus plantarum and bacillus subtilis；
Step 2: a variety of bacteria suspensions made of step 1 are poured into graduated cylinder, and are uniformly mixed, compound bacteria is obtained；
Step 3: taking wheat bran, zein and diatomite spare in proportion, and the wheat bran, zein and diatomite of preparation are stirred
Mixing is mixed, powder carrier is obtained；
Step 4: the composite bacteria liquid that step 2 obtains is admixed and is uniformly mixed with the powder carrier that step 3 obtains, i.e.,
Water remediation microbial inoculum semi-finished product can be obtained；
Step 5: stabilizer is added in the water remediation microbial inoculum semi-finished product prepared in step 4, stabilizer is set as benzene
Sodium formate, and the dosage of stabilizer is set as 0.2kg, obtains water remediation microbial inoculum finished product after mixing again.
It further include the application method of landscape water body remediation microbial inoculum, specifically used step are as follows: water remediation microbial inoculum finished product makes
6h is activated at room temperature using molasses water with preceding.
Embodiment 6:
The present invention provides a kind of formula of landscape water body remediation microbial inoculum, including carrier, the major ingredient that the carrier uses is pressed
Poidometer includes: wheat bran 3kg, zein 1kg and diatomite 6kg, further includes compound bacteria 1kg；
Further, the compound bacteria is by Rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, fibrosis promise Cattell
Bacterium, lactobacillus plantarum and bacillus subtilis composition, the Rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, fibrosis
Nocard's bacillus, lactobacillus plantarum and the setting of the mixing mass ratio of bacillus subtilis are as follows: 4:4:3:6:6:4；
Further, the Rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, fibrosis Nocard's bacillus, plant cream
Bacillus and bacillus subtilis are used to prepare bacteria suspension；
Further, the viable count of a variety of bacteria suspensions is disposed as 1.0 × 108cfu/mL。
A kind of preparation method of landscape water body remediation microbial inoculum, concrete operation step are as follows:
Step 1: culture bacterium is taken from the bacterium colony in culture medium with oese, is placed in the test tube equipped with sterile water, with strength
Mix, prepare bacteria suspension, and in proportion by Rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, fibrosis Nocard's bacillus,
Bacteria suspension is respectively prepared in lactobacillus plantarum and bacillus subtilis；
Step 2: a variety of bacteria suspensions made of step 1 are poured into graduated cylinder, and are uniformly mixed, compound bacteria is obtained；
Step 3: taking wheat bran, zein and diatomite spare in proportion, and the wheat bran, zein and diatomite of preparation are stirred
Mixing is mixed, powder carrier is obtained；
Step 4: the composite bacteria liquid that step 2 obtains is admixed and is uniformly mixed with the powder carrier that step 3 obtains, i.e.,
Water remediation microbial inoculum semi-finished product can be obtained；
Step 5: stabilizer is added in the water remediation microbial inoculum semi-finished product prepared in step 4, stabilizer is set as mountain
Potassium sorbate, and the dosage of stabilizer is set as 0.2kg, obtains water remediation microbial inoculum finished product after mixing again.
It further include the application method of landscape water body remediation microbial inoculum, specifically used step are as follows: water remediation microbial inoculum finished product makes
6h is activated at room temperature using molasses water with preceding.
Embodiment 7:
Item as a comparison, in order to prove that the water remediation microbial inoculum of the above embodiments 1-6 offer repairs the effect of polluted-water
Fruit, the present embodiment choose embodiment 1-6 provide landscape water body remediation microbial inoculum tested as sample, and comparison of design example into
Row check experiment.It should be noted that any microbial inoculum and medicament are not added in comparative example, other experimental conditions and embodiment phase
Together, the test data obtained is as follows:
Ammonia-nitrogen content situation of change in 1 water of table
| Test group | Before test (mg/L) | After test (mg/L) | Removal rate (%) |
| Embodiment 7 | 5.64 | 5.31 | 5.9 |
| Embodiment 1 | 5.64 | 2.73 | 51.6 |
| Embodiment 2 | 5.64 | 2.62 | 53.5 |
| Embodiment 3 | 5.64 | 2.78 | 50.7 |
| Embodiment 4 | 5.64 | 2.81 | 50.2 |
| Embodiment 5 | 5.64 | 2.58 | 54.3 |
| Embodiment 6 | 5.64 | 2.64 | 53.2 |
Total nitrogen content situation of change in 2 water of table
| Embodiment 7 | Before test (mg/L) | After test (mg/L) | Removal rate (%) |
| Comparative example | 2.73 | 2.63 | 3.7 |
| Embodiment 1 | 2.73 | 1.91 | 30.0 |
| Embodiment 2 | 2.73 | 1.88 | 31.1 |
| Embodiment 3 | 2.73 | 1.79 | 34.4 |
| Embodiment 4 | 2.73 | 1.75 | 35.9 |
| Embodiment 5 | 2.73 | 1.68 | 38.5 |
| Embodiment 6 | 2.73 | 1.72 | 37.0 |
Total phosphorus content situation of change in 3 water of table
| Embodiment 7 | Before test (mg/L) | After test (mg/L) | Removal rate (%) |
| Comparative example | 0.65 | 0.59 | 9.2 |
| Embodiment 1 | 0.65 | 0.36 | 44.6 |
| Embodiment 2 | 0.65 | 0.35 | 46.2 |
| Embodiment 3 | 0.65 | 0.34 | 47.7 |
| Embodiment 4 | 0.65 | 0.32 | 50.8 |
| Embodiment 5 | 0.65 | 0.31 | 52.3 |
| Embodiment 6 | 0.65 | 0.28 | 56.9 |
Permanganate index changes of contents in 4 water of table
As seen from the above table, the harmful substance in contaminant water can be degraded and be converted to composite bacteria agent of the present invention, promote indigenous flora
Growth, play the role of purifying water, and harmful substance removal rate highest in the water in embodiment 5, water body purification effect is most
It is good, Rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, fibrosis Nocard's bacillus, lactobacillus plantarum and bacillus subtilis
Preferably proportionate relationship is 2:2:1:2:4:1, realizes microbial inoculum to the optimization of water remediation effect, Rhodopseudomonas palustris utilizes
Organic matter carries out photosynthesis, improves Dissolved Oxygen in Water content, to improve water body self-purification ability, realize fixed nitrogen, carbon sequestration and take off
Hydrogen effect, degrade sewage in multiple harmful substances, nitrobacteria by Nitrite transformation in water be nitrate, promote water body in have
The growth of beneficial algae reduces the alkaline matter in water body, reduces water pH value, denitrifying bacteria consumes the nitrogen of water body, to cut
The source of disconnected algae nitrogen nutrition, inhibits Measures of Algae in Water Body excessive multiplication, achievees the purpose that purifying water body, fibrosis Nocard's bacillus
Enhance bed mud fertility, promote the growth of plant itself, inhibits harmful bacteria growth in water, lactobacillus plantarum during the growth process can be big
Volume production acid, stablizes the pH value in water, heavy metal of degrading, and reduces the content of poisonous metal in water, and bacillus subtilis inhibits fungi
Disease inhibits the harmful microorganisms such as vibrios, Escherichia coli and baculoviral in water body, decomposes the organic matter and nuisance in water body
Matter purifies water, and provides carrier and is limited compound bacteria in water remediation microbial inoculum and the proportionate relationship of carrier, maintains water
The balanced relation of compound bacteria and microbe carrier considers to interact between the special role and flora of each flora in body remediation microbial inoculum
While, so that each flora is maintained relatively steady state.
Finally, it should be noted that the foregoing is only a preferred embodiment of the present invention, it is not intended to restrict the invention,
All within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in of the invention
Within protection scope.
Claims (8)
1. a kind of formula of landscape water body remediation microbial inoculum, including carrier, it is characterised in that: the major ingredient that the carrier uses is by weight
Part meter includes: 10-30 parts of wheat bran, 6-10 parts of zein and 40-60 parts of diatomite, further includes 8-10 parts of compound bacteria.
2. a kind of formula of landscape water body remediation microbial inoculum according to claim 1, it is characterised in that: what the carrier used
Major ingredient includes: wheat bran 3-10kg, zein 2-3kg and diatomite 6-20kg by weight, further includes compound bacteria 1-4kg.
3. a kind of formula of landscape water body remediation microbial inoculum according to claim 1, it is characterised in that: the compound bacteria is by natural pond
Damp red pseudomonas, nitrobacteria, denitrifying bacteria, fibrosis Nocard's bacillus, lactobacillus plantarum and bacillus subtilis composition,
The Rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, fibrosis Nocard's bacillus, lactobacillus plantarum and bacillus subtilis
The mixing mass ratio of bacterium is arranged are as follows: 2-4:2-4:1-3:2-6:4-6:1-4.
4. a kind of formula of landscape water body remediation microbial inoculum according to claim 3, it is characterised in that: the marsh is red false single
Born of the same parents bacterium, nitrobacteria, denitrifying bacteria, fibrosis Nocard's bacillus, lactobacillus plantarum and bacillus subtilis are used to preparation bacterium
Suspension.
5. a kind of formula of landscape water body remediation microbial inoculum according to claim 4, it is characterised in that: a variety of bacteria suspensions
Viable count be disposed as 1.0 × 108-1.0×109cfu/mL。
6. a kind of preparation method of landscape water body remediation microbial inoculum, it is characterised in that: concrete operation step are as follows:
Step 1: culture bacterium is taken from the bacterium colony in culture medium with oese, is placed in the test tube equipped with sterile water, stirs with strength
With, prepare bacteria suspension, and in proportion by Rhodopseudomonas palustris, nitrobacteria, denitrifying bacteria, fibrosis Nocard's bacillus, plant
Bacteria suspension is respectively prepared in object lactobacillus and bacillus subtilis；
Step 2: a variety of bacteria suspensions made of step 1 are poured into graduated cylinder, and are uniformly mixed, compound bacteria is obtained；
Step 3: taking wheat bran, zein and diatomite spare in proportion, the wheat bran, zein and diatomite of preparation is stirred mixed
It closes, obtains powder carrier；
Step 4: the composite bacteria liquid that step 2 obtains is admixed and is uniformly mixed with the powder carrier that step 3 obtains, can be obtained
To water remediation microbial inoculum semi-finished product；
Step 5: stabilizer is added in the water remediation microbial inoculum semi-finished product prepared in step 4, obtains after mixing again
Water remediation microbial inoculum finished product.
7. a kind of preparation method of landscape water body remediation microbial inoculum according to claim 6, it is characterised in that: the step 5
Middle stabilizer is set as one of potassium sorbate or sodium benzoate, the addition of the stabilizer and water remediation microbial inoculum semi-finished product
Ratio setting is 1:55.
8. a kind of preparation method of landscape water body remediation microbial inoculum according to claim 6, it is characterised in that: further include landscape
The application method of water remediation microbial inoculum, specifically used step are as follows: water remediation microbial inoculum finished product is utilizing molasses water in room using preceding
4-6h is activated under the conditions of temperature.
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Application publication date: 20191029 |