CN112226431A - Preparation method of composite filler loaded with functional microorganisms - Google Patents
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Abstract
The invention relates to the technical field of waste gas treatment, in particular to a preparation method of a composite filler loaded with functional microorganisms. The functional microorganism which is obtained by domestication and can effectively degrade methanol, toluene and xylene is inoculated into sterilized nutrient substances for culture, then is uniformly mixed with agar powder, activated carbon powder and polyvinyl alcohol, and is pressed into composite filler particles by a mould. The filler is used for treating chemical waste gas VOCs, the VOCs degradation efficiency is high, nutrient substances can be slowly released, the restart time after the start and shutdown maintenance of the biological trickling filter can be shortened, and the filler is simple in preparation method, low in cost, stable in operation effect, free of secondary pollution after waste gas treatment and environment-friendly.
Description
Technical Field
The invention relates to the technical field of waste gas treatment, in particular to a preparation method of a composite filler loaded with functional microorganisms.
Background
Volatile organic pollutants (VOCs) are classified as one of the new types of atmospheric pollutants because of their toxicity and potential contribution to global warming. The sources of VOCs are mainly divided into natural sources and artificial sources, and the artificial sources are mainly volatilization and leakage of organic solvents widely used in the synthesis and extraction processes of chemical industry, pharmaceutical industry and the like. The excessive concentration of VOCs can not only stimulate the respiratory system of the human body, but also cause chronic and acute poisoning of the human body and even cause 'three-cause' effect. Therefore, it is urgent to find an economical and efficient means for removing VOCs.
Along with the continuous improvement of environmental protection requirement, especially the exhaust emission standard who produces in the production life continuously improves, brings certain challenge to the processing of VOCs in the chemical waste gas, uses traditional treatment process in the chemical waste gas VOCs treatment now: the process comprises the steps of activated carbon adsorption, acid washing and alkaline washing, RTO, RCO, plasma oxidation, traditional biological deodorization and the like, can solve certain types of waste gas, but has nonideal treatment effect and efficiency on VOCs in chemical waste gas with complex components, and has higher investment and operation cost.
At present, the biological treatment technology is known as the optimal treatment technology under the condition of large-volume exhaust emission of low-concentration VOCs, compared with physical and chemical technologies, the environmental impact and the operation cost of the biological treatment technology are lower, a biological filtration method and a biological trickling filtration method are the most common processes for biological treatment of exhaust gas at present, wherein the biological trickling filtration method is the improvement of the biological filtration process, can accurately control the environmental conditions, has high biological quantity attached to a unit volume filler, is the most widely applied biological treatment process for removing industrial exhaust gas at present, and is paid more attention by more and more students at home and abroad.
However, the bio-trickling filtration is often limited by the ability of VOCs to migrate into the microorganisms or the maximum conversion of contaminants by the microorganisms, and is limited by mass transfer processes or reaction processes during operation, resulting in a reduction in treatment efficiency.
In order to solve the limitation of the mass transfer process, the search for a filler with excellent mass transfer performance and processing capacity is very important. At present, the commonly used industrial bio-trickling filter fillers include ceramsite, perlite, small polypropylene balls, plastic rings, compost, wood chips and the like. They are either smooth in surface, not conducive to biofilm formation by microorganisms, and do not provide any nutrients for microbial growth; or the biological packed tower can be decomposed and compacted after running for a period of time, the natural shape and structure are not favorable for uniform distribution of airflow, and blockage is easy to occur, so that the purification capacity of the biological packed tower is greatly reduced. In addition, the common fillers do not carry microorganisms, a long time is needed for domesticating the biofilm formation process in the initial running stage, the starting time of the biological tower is prolonged, the complexity of system operation is increased, and the restarting time needed by the biological trickling filter tower is also long after production halt or maintenance. Patent CN 110772975 a discloses a module combined biological deodorization device and its manufacturing and using method, which adopts the traditional biological filter bed process, but does not essentially solve the problem of mass transfer of pollutants; patent CN 106914127B discloses a method for treating nitrobenzene waste gas by a coupling bacteria biological filter bed, polyurethane foam is used as a filler for biological film formation, and the singly selected filler is easy to cause filler blockage after long-time operation, so that the mass transfer treatment efficiency is influenced; patent CN 110772971A discloses a modularized microorganism filter bed and a manufacturing method thereof, wherein a filler adopts a combination of PE polyhedral spheres, porous ceramsite and bamboo charcoal, the mass transfer problem is solved to a certain extent, and a longer biofilm formation starting time is still needed.
Based on the above situation, it is important to develop a novel composite filler loaded with highly effective functional microorganisms for specific waste gas VOCs components by combining the prior art. The invention provides a preparation method of a composite filler loaded with functional microorganisms, which is characterized in that a biological source rich in the functional microorganisms is fixed in the filler and on the surface of the filler in the preparation process of the filler by utilizing an embedding immobilization technology, so that the effective amount of the microorganisms on the filler is increased, and the start time of biofilm formation of a biological trickling filter is greatly shortened; in the creation of a micro environment for the growth of microorganisms, an inorganic and organic material composite technology is adopted, so that the biological trickling filter has nutrient slow-release substances required by the growth of the microorganisms, has enough specific surface area for the growth and the propagation of the microorganisms, and greatly shortens the restarting time of the biological trickling filter after production stoppage and maintenance.
Disclosure of Invention
The invention aims to provide a preparation method of a composite filler loaded with functional microorganisms, which comprises the following steps: adding distilled water into beef extract, peptone, sodium chloride, magnesium sulfate, zinc chloride and potassium dihydrogen phosphate for dissolving, stirring uniformly, sterilizing, inoculating domesticated functional microorganisms for efficiently degrading methanol, toluene and xylene, and performing shaking culture to obtain a functional microorganism mixed solution for efficiently degrading methanol, toluene and xylene; and mixing agar powder, activated carbon powder and polyvinyl alcohol with distilled water, stirring, heating and dissolving, then uniformly mixing with the functional microorganism mixed solution, and pressing by using a die to obtain the functional microorganism-loaded composite filler particles, wherein the functional microorganism-loaded composite filler is loaded in a biological trickling filter tower and used for absorbing and treating chemical waste gas VOCs.
Wherein the mass ratio of the beef extract to the peptone to the sodium chloride to the magnesium sulfate to the zinc chloride to the monopotassium phosphate to the agar powder to the activated carbon powder to the polyvinyl alcohol is 0.2-0.3: 0.8-1.0: 0.4-0.5: 0.5-1.0: 0.004-0.0045: 0.5-1.0: 1.5-2.5: 1.0-1.5: 15-35; preferably, the mass ratio of the beef extract to the peptone to the sodium chloride to the magnesium sulfate to the zinc chloride to the monopotassium phosphate to the agar powder to the activated carbon powder to the polyvinyl alcohol is 0.3: 1.0: 0.5: 0.5: 0.0045: 0.8: 2.0: 1.2: 30.
Further, the mass volume ratio (g/L) of the mixture of the beef extract, the peptone, the sodium chloride, the magnesium sulfate, the zinc chloride and the monopotassium phosphate to the distilled water is 2.4-3.8: 0.1, and the mass volume ratio (g/L) of the mixture of the agar powder, the activated carbon powder and the polyvinyl alcohol to the distilled water is 17.5-39.0: 0.3; preferably, the mass volume ratio (g/L) of the mixture of the beef extract, the peptone, the sodium chloride, the magnesium sulfate, the zinc chloride and the monopotassium phosphate to the distilled water is 3.8: 1.0, and the mass volume ratio (g/L) of the mixture of the agar powder, the activated carbon powder and the polyvinyl alcohol to the distilled water is 35.0: 0.3.
Further, the inoculated methanol, toluene and xylene domesticated functional microorganisms for efficiently degrading methanol, toluene and xylene comprise Chryseobacterium, Bacteroides, Acidobacterium, denitrifying phosphorus-accumulating bacteria, Methylophilus, Aphyllophorales, Chitinophaga, alpha-Proteus, Pythium, P,Lacibacterium、Litorilinea、Methyloversatilis、OhtaekwangiaAndAnaerolineaceaethe mass ratio of the 14 bacteria (in the above order) was 1.5: 3.5: 1.3: 7.5: 15.0: 2.5: 2.1: 5.5: 2.0: 3.8: 5.5: 8.6: 3.0: 13.3.
Furthermore, the inoculation amount (by the volume of the bacterial liquid) of the functional microorganism mixed bacterial liquid is 10% of the volume of the mixed liquid of the beef extract, the peptone, the sodium chloride, the magnesium sulfate, the zinc chloride and the monopotassium phosphate.
Further, the rotation speed of the mixed liquid inoculated with the functional microorganisms capable of efficiently degrading methanol, toluene and xylene in a constant-temperature shaking table during shaking culture is 180r/min, the temperature is 28 ℃, and the culture time is 1-3 days.
The invention has the beneficial effects that:
1. the invention provides a preparation method of a functional microorganism-loaded composite filler, which adopts inorganic and organic material composite technology, the prepared filler has enough specific surface area, and any microorganism grows and breeds in large quantity, and waste gas can uniformly pass through the filler by adopting different stacking and filling modes according to actual requirements, so that the mass transfer problem of pollutants between the filler and the microorganism is basically solved, the normal treatment effect of a system can be kept for a long time due to the existence of a large quantity of microorganisms, and the waste gas treatment is ensured to reach the standard and be discharged;
2. according to the preparation method of the composite filler loaded with the functional microorganisms, provided by the invention, by utilizing an embedding immobilization technology, a biological source rich in the functional microorganisms which are domesticated by methanol, toluene and xylene and have higher degradation efficiency is fixed in and on the filler in the preparation process of the filler, the pertinence to the degradation of the methanol, the toluene and the xylene is strong, the treatment effect is good, the domestication film hanging time of a start-up stage of a biotrickling filter can be effectively reduced, and the operation cost is low;
3. according to the preparation method of the composite filler loaded with the functional microorganisms, the prepared composite filler contains slowly-releasing nutrient substances (beef extract, peptone, sodium chloride, magnesium sulfate, zinc chloride and monopotassium phosphate), and the activity of the microorganisms can be maintained by the nutrient substances in the filler under the condition of no external nutrient sources such as production halt, maintenance and the like, so that the time required by restarting the biological trickling filter tower is shortened.
Detailed Description
Example 1
The waste gas VOCs is mainly methanol.
The implementation mode is as follows:
(1) acclimatization of functional microorganisms with methanol:
a. weighing 0.8g of peptone, 0.4g of sodium chloride, 0.5g of magnesium sulfate, 0.004g of zinc chloride and 0.5g of monopotassium phosphate, measuring 2ml of methanol (simulating the main component of waste gas VOCs), adding 100ml of distilled water, stirring uniformly, and sterilizing for later use;
b. taking Flavobacterium, Bacteroides, Acidobacterium, denitrifying phosphorus-accumulating bacterium, Methylophilus, Phytophthora, Chitinophaga, alpha-Proteus, Pythium, etc,Lacibacterium、Litorilinea、Methyloversatilis、OhtaekwangiaAndAnaerolineaceaeuniformly mixing bacterial liquid according to the mass ratio of strains of 1.5: 3.5: 1.3: 7.5: 15.0: 2.5: 2.1: 5.5: 2.0: 3.8: 5.5: 8.6: 3.0: 13.3, inoculating 10ml of the mixed bacterial liquid into the solution a, and carrying out shaking culture at 28 ℃ and 180r/min in a constant-temperature incubator for 3-5 days;
c. measuring the content of methanol by taking the solution cultured in the previous step, when the removal rate of the methanol reaches more than 90%, taking 10ml of the bacterial liquid in the previous step, inoculating the bacterial liquid into the solution a which is prepared freshly, adding 2ml of methanol, and performing shaking culture for 3-5 days at 28 ℃ and 180r/min in a constant-temperature incubator;
d. repeating the step c for one to three times, and obtaining the functional microorganism mixed liquor for efficiently degrading the methanol when the removal rate of the methanol is stable to more than 90 percent.
(2) Weighing 0.2g of beef extract, 0.8g of peptone, 0.4g of sodium chloride, 0.5g of magnesium sulfate, 0.004g of zinc chloride, 0.5g of monopotassium phosphate, 1.5g of agar, 1.0g of activated carbon powder and 15g of polyvinyl alcohol;
(3) adding 100mL of distilled water into the weighed nutrient substances (beef extract, peptone, sodium chloride, magnesium sulfate, zinc chloride and potassium dihydrogen phosphate) to dissolve, stirring uniformly, and sterilizing for later use;
(4) inoculating the mixed solution of the domesticated and cultured functional microorganisms in the step (1) according to 10% of the volume of the solution in the step (3), and carrying out shake culture in a constant-temperature shaking table for 1-3 days at the rotation speed of 180r/min and the temperature of 28 ℃;
(5) adding 300ml of distilled water into the agar powder, the activated carbon powder and the polyvinyl alcohol, heating, stirring and dissolving, and cooling for later use;
(6) uniformly mixing the functional microorganism mixed solution cultured in the step (4) with the solution in the step (5), preparing filler particles by using a mold, and forming for later use after cooling;
(7) the prepared filler is filled into a small-scale biological trickling filter of a waste gas treatment system and is divided into an upper module and a lower module for filling, the porosity of the filler filled in the lower layer of the module 1 is 38%, the porosity of the filler filled in the middle layer is 32%, the porosity of the filler filled in the upper layer is 25%, and the filling mode of the module 2 is the same as that of the module 1.
After the biotrickling filter was operated for a period of time, the biotrickling filter was found to have a methanol removal rate of 99.1%.
Example 2
The waste gas VOCs are mainly methanol and toluene.
The implementation mode is as follows:
(1) acclimatization of functional microorganisms with methanol and toluene:
a. weighing 0.9g of peptone, 0.45g of sodium chloride, 0.75g of magnesium sulfate, 0.0043g of zinc chloride and 0.8g of monopotassium phosphate, weighing 2ml of methanol and toluene (namely the main components of waste gas VOCs), adding 100ml of distilled water, stirring uniformly, and sterilizing for later use;
b. taking Flavobacterium, Bacteroides, Acidobacterium, denitrifying phosphorus-accumulating bacterium, Methylophilus, Phytophthora, Chitinophaga, alpha-Proteus, Pythium, etc,Lacibacterium、Litorilinea、Methyloversatilis、OhtaekwangiaAndAnaerolineaceaeuniformly mixing bacterial liquid according to the mass ratio of strains of 1.5: 3.5: 1.3: 7.5: 15.0: 2.5: 2.1: 5.5: 2.0: 3.8: 5.5: 8.6: 3.0: 13.3, inoculating 10ml of the mixed bacterial liquid into the solution a, and carrying out shaking culture at 28 ℃ and 180r/min in a constant-temperature incubator for 3-5 days;
c. taking the cultured solution in the previous step to respectively measure the content of methanol and toluene, taking 10ml of the bacterial liquid in the previous step to inoculate the bacterial liquid into the freshly prepared solution a when the removal rate of the methanol and the removal rate of the toluene respectively reach more than 90%, adding 2ml of methanol and toluene respectively, and carrying out shaking culture at 28 ℃ and 180r/min in a constant-temperature incubator for 3-5 days;
d. and c, repeating the step c for one to three times, and obtaining the functional microorganism mixed liquor for efficiently degrading the methanol and the toluene when the removal rates of the methanol and the toluene respectively reach more than 90 percent stably.
(2) Weighing 0.25g of beef extract, 0.9g of peptone, 0.45g of sodium chloride, 0.75g of magnesium sulfate, 0.0043g of zinc chloride, 0.8g of monopotassium phosphate, 2.0g of agar, 1.3g of activated carbon powder and 28g of polyvinyl alcohol;
(3) adding 100mL of distilled water into the weighed nutrient substances (beef extract, peptone, sodium chloride, magnesium sulfate, zinc chloride and potassium dihydrogen phosphate) to dissolve, stirring uniformly, and sterilizing for later use;
(4) inoculating the mixed solution of the domesticated and cultured functional microorganisms in the step (1) according to 10% of the volume of the solution in the step (3), and carrying out shake culture in a constant-temperature shaking table for 1-3 days at the rotation speed of 180r/min and the temperature of 28 ℃;
(5) adding 300ml of distilled water into the agar powder, the activated carbon powder and the polyvinyl alcohol, heating, stirring and dissolving, and cooling for later use;
(6) uniformly mixing the functional microorganism mixed solution cultured in the step (4) with the solution in the step (5), preparing filler particles by using a mold, and forming for later use after cooling;
(7) the prepared filler is filled into a small-scale biological trickling filter of a waste gas treatment system and is divided into an upper module and a lower module for filling, the porosity of the filler filled in the lower layer of the module 1 is 38%, the porosity of the filler filled in the middle layer is 32%, the porosity of the filler filled in the upper layer is 25%, and the filling mode of the module 2 is the same as that of the module 1.
After the biotrickling filter operates for a period of time, the removal rates of the biotrickling filter to methanol and toluene are respectively measured to be 98.6 percent and 92.3 percent; the biological trickling filter is restarted after stopping running for one month, low-concentration waste gas containing methanol and toluene is introduced, the removal rates of the methanol and the toluene reach 94.4 percent and 90.1 percent respectively after running for 5 days, and the treatment level before stopping running is basically recovered.
Example 3
The waste gas VOCs are mainly methanol, toluene and xylene.
The implementation mode is as follows:
(1) acclimatization of functional microorganisms with methanol, toluene and xylene:
a. weighing 1.0g of peptone, 0.5g of sodium chloride, 1.0g of magnesium sulfate, 0.0045g of zinc chloride and 1.0g of monopotassium phosphate, measuring 2ml of methanol, toluene and xylene (namely the main components of waste gas VOCs), adding 100ml of distilled water, stirring uniformly, and sterilizing for later use;
b. taking Flavobacterium, Bacteroides, Acidobacterium, denitrifying phosphorus-accumulating bacterium, Methylophilus, Phytophthora, Chitinophaga, alpha-Proteus, Pythium, etc,Lacibacterium、Litorilinea、Methyloversatilis、OhtaekwangiaAndAnaerolineaceaeuniformly mixing bacterial liquid according to the mass ratio of strains of 1.5: 3.5: 1.3: 7.5: 15.0: 2.5: 2.1: 5.5: 2.0: 3.8: 5.5: 8.6: 3.0: 13.3, inoculating 10ml of the mixed bacterial liquid into the solution a, and carrying out shaking culture at 28 ℃ and 180r/min in a constant-temperature incubator for 3-5 days;
c. taking the solution cultured in the previous step to respectively measure the contents of methanol, toluene and xylene, taking 10ml of the bacterial liquid in the previous step to inoculate the solution a which is prepared freshly when the removal rates of the methanol, the toluene and the xylene respectively reach more than 90%, 90% and 80%, adding 2ml of methanol, the toluene and the xylene respectively, and carrying out shaking culture at 28 ℃ and 180r/min in a constant-temperature incubator for 3-5 days;
d. and c, repeating the step c for one to three times, and obtaining the functional microorganism mixed liquor for efficiently degrading the methanol, the toluene and the xylene when the removal rates of the methanol, the toluene and the xylene respectively reach 90%, 90% and more than 80%.
(2) Weighing 0.3g of beef extract, 1.0g of peptone, 0.5g of sodium chloride, 1.0g of magnesium sulfate, 0.0045g of zinc chloride, 1.0g of monopotassium phosphate, 2.5g of agar, 1.5g of activated carbon powder and 35g of polyvinyl alcohol;
(3) adding 100mL of distilled water into the weighed nutrient substances (beef extract, peptone, sodium chloride, magnesium sulfate, zinc chloride and potassium dihydrogen phosphate) to dissolve, stirring uniformly, and sterilizing for later use;
(4) inoculating the mixed solution of the domesticated and cultured functional microorganisms in the step (1) according to 10% of the volume of the solution in the step (3), and carrying out shake culture in a constant-temperature shaking table for 1-3 days at the rotation speed of 180r/min and the temperature of 28 ℃;
(5) adding 300ml of distilled water into the agar powder, the activated carbon powder and the polyvinyl alcohol, heating, stirring and dissolving, and cooling for later use;
(6) uniformly mixing the functional microorganism mixed solution cultured in the step (4) with the solution in the step (5), preparing filler particles by using a mold, and forming for later use after cooling;
(7) the prepared filler is filled into a small-scale biological trickling filter of a waste gas treatment system and is divided into an upper module and a lower module for filling, the porosity of the filler filled in the lower layer of the module 1 is 38%, the porosity of the filler filled in the middle layer is 32%, the porosity of the filler filled in the upper layer is 25%, and the filling mode of the module 2 is the same as that of the module 1.
After the biotrickling filter was operated for a period of time, the biotrickling filter was found to have a removal rate of methanol, toluene and xylene of 98.1%, 93.5% and 82.7%, respectively.
Claims (6)
1. A preparation method of a composite filler loaded with functional microorganisms is characterized in that distilled water is added into beef extract, peptone, sodium chloride, magnesium sulfate, zinc chloride and potassium dihydrogen phosphate for dissolving, the mixture is uniformly stirred, domesticated functional microorganisms for degrading methanol, toluene and xylene are inoculated after sterilization, and a functional microorganism mixed solution is obtained by shaking culture; and mixing agar powder, activated carbon powder and polyvinyl alcohol with distilled water, stirring, heating and dissolving, then uniformly mixing with the functional microorganism mixed solution, and pressing by using a die to obtain composite filler particles loaded with the functional microorganism, wherein the composite filler is loaded in a biological trickling filter to absorb and treat chemical waste gas VOCs.
2. The method for preparing the functional microorganism-loaded composite filler according to claim 1, wherein the mass ratio of the beef extract, the peptone, the sodium chloride, the magnesium sulfate, the zinc chloride, the monopotassium phosphate, the agar powder, the activated carbon powder and the polyvinyl alcohol is 0.2-0.3: 0.8-1.0: 0.4-0.5: 0.5-1.0: 0.004-0.0045: 0.5-1.0: 1.5-2.5: 1.0-1.5: 15-35.
3. The preparation method of the functional microorganism-loaded composite filler, according to claim 1, is characterized in that the mass-to-volume ratio of the mixture of beef extract, peptone, sodium chloride, magnesium sulfate, zinc chloride and monopotassium phosphate to distilled water is 2.4-3.8: 0.1; the mass-volume ratio of the mixture of the agar powder, the activated carbon powder and the polyvinyl alcohol to the distilled water is 17.5-39.0: 0.3.
4. The method for preparing the composite filler loaded with the functional microorganisms according to claim 1, wherein the domesticated functional microorganisms for degrading methanol, toluene and xylene comprise Chryseobacterium, Bacteroides, Acidobacterium, denitrifying polyphosphate bacteria, Methylophilus, Phytophthora, Chitinophaga, Alphaera, Plough, Aureobasidium, Auricularia, and Aureobasidium,Lacibacterium、Litorilinea、Methyloversatilis、OhtaekwangiaAndAnaerolineaceaethe mass ratio of the 14 bacteria is 1.5: 3.5: 1.3: 7.5: 15.0: 2.5: 2.1: 5.5: 2.0: 3.8: 5.5: 8.6: 3.0: 13.3.
5. The method for preparing a composite filler loaded with functional microorganisms according to claim 1, wherein the inoculation volume of the mixed bacterial solution of functional microorganisms is 10% of the volume of the mixed liquid of beef extract, peptone, sodium chloride, magnesium sulfate, zinc chloride and potassium dihydrogen phosphate.
6. The preparation method of the composite filler loaded with the functional microorganisms, according to claim 1, is characterized in that the rotation speed of the mixed solution inoculated with the functional microorganisms capable of degrading methanol, toluene and xylene in a constant temperature shaking table during shaking culture is 180r/min, the temperature is 28 ℃, and the culture time is 1-3 days.
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CN115814584A (en) * | 2022-10-19 | 2023-03-21 | 北京工业大学 | Diatomite-based composite filler for reinforcing performance of biological trickling filter |
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Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105087541A (en) * | 2015-09-07 | 2015-11-25 | 博天环境集团股份有限公司 | Microbe immobilizing method |
CN110699347A (en) * | 2019-08-05 | 2020-01-17 | 中电建生态环境集团有限公司 | Immobilized microbial inoculum and preparation method and application thereof |
CN111333200A (en) * | 2020-03-18 | 2020-06-26 | 运城学院 | Embedded immobilized microorganism particles, preparation method and sewage treatment method |
-
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Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105087541A (en) * | 2015-09-07 | 2015-11-25 | 博天环境集团股份有限公司 | Microbe immobilizing method |
CN110699347A (en) * | 2019-08-05 | 2020-01-17 | 中电建生态环境集团有限公司 | Immobilized microbial inoculum and preparation method and application thereof |
CN111333200A (en) * | 2020-03-18 | 2020-06-26 | 运城学院 | Embedded immobilized microorganism particles, preparation method and sewage treatment method |
Non-Patent Citations (1)
Title |
---|
石若夫: "《微生物学实验技术》", 31 August 2017, 北京航空航天大学出版社 * |
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CN115814584A (en) * | 2022-10-19 | 2023-03-21 | 北京工业大学 | Diatomite-based composite filler for reinforcing performance of biological trickling filter |
CN115814584B (en) * | 2022-10-19 | 2024-06-04 | 北京工业大学 | Diatomite-based composite filler for strengthening performance of biotrickling filter |
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