CN115141724B - Stamp type strain inoculation bag for substance bacteriostasis experiment - Google Patents
Stamp type strain inoculation bag for substance bacteriostasis experiment Download PDFInfo
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- CN115141724B CN115141724B CN202210690569.XA CN202210690569A CN115141724B CN 115141724 B CN115141724 B CN 115141724B CN 202210690569 A CN202210690569 A CN 202210690569A CN 115141724 B CN115141724 B CN 115141724B
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- 238000011081 inoculation Methods 0.000 title claims abstract description 75
- 238000002474 experimental method Methods 0.000 title claims abstract description 17
- 239000000126 substance Substances 0.000 title claims description 8
- 239000007788 liquid Substances 0.000 claims abstract description 214
- 241000233866 Fungi Species 0.000 claims abstract description 205
- 238000007598 dipping method Methods 0.000 claims abstract description 173
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 45
- 241000894006 Bacteria Species 0.000 claims abstract description 27
- 239000000463 material Substances 0.000 claims abstract description 7
- 239000002775 capsule Substances 0.000 claims description 50
- 239000012528 membrane Substances 0.000 claims description 27
- 239000007787 solid Substances 0.000 claims description 9
- 229910000838 Al alloy Inorganic materials 0.000 claims description 8
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 8
- 229910000881 Cu alloy Inorganic materials 0.000 claims description 8
- 239000010949 copper Substances 0.000 claims description 8
- 239000010935 stainless steel Substances 0.000 claims description 8
- 229910001256 stainless steel alloy Inorganic materials 0.000 claims description 8
- 230000005484 gravity Effects 0.000 claims description 6
- 239000004033 plastic Substances 0.000 claims description 5
- 239000005060 rubber Substances 0.000 claims description 5
- 230000009471 action Effects 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- 239000000919 ceramic Substances 0.000 claims description 3
- 239000000741 silica gel Substances 0.000 claims description 3
- 229910002027 silica gel Inorganic materials 0.000 claims description 3
- -1 vitreous Substances 0.000 claims description 3
- 238000009987 spinning Methods 0.000 claims 2
- 239000001963 growth medium Substances 0.000 abstract description 23
- 230000001580 bacterial effect Effects 0.000 abstract description 19
- 238000000576 coating method Methods 0.000 abstract description 11
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- 238000012258 culturing Methods 0.000 description 5
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- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 230000000844 anti-bacterial effect Effects 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 3
- 235000017491 Bambusa tulda Nutrition 0.000 description 3
- 241001330002 Bambuseae Species 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 3
- 239000000853 adhesive Substances 0.000 description 3
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- 238000010586 diagram Methods 0.000 description 3
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- 238000004659 sterilization and disinfection Methods 0.000 description 3
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- 229920001817 Agar Polymers 0.000 description 2
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- 230000005764 inhibitory process Effects 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000005096 rolling process Methods 0.000 description 2
- 238000007790 scraping Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 210000003813 thumb Anatomy 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
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- 229920000742 Cotton Polymers 0.000 description 1
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- 238000010438 heat treatment Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 210000004932 little finger Anatomy 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 238000013316 zoning Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
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Abstract
Stamp type bacterial inoculation bag for material bacteriostasis experiments belongs to the technical field of biology, and comprises a bacterium dipping liquid bag (1) and a bacterium dipping liquid disc (8), and is characterized in that: the fungus liquid dipping device is characterized in that the fungus liquid dipping bag (1) is an inoculation bag which is used for dipping fungus liquid in the fungus liquid dipping tray (8) and then inoculating the fungus liquid to a culture plate, the fungus liquid dipping tray (8) is a concave tray which is used for containing fungus liquid to be inoculated and is used for dipping the fungus liquid, the fungus liquid dipping bag (1) is dipped by utilizing thin-wall silicone with fungus liquid film protruding on the outer wall of water, micro fungus liquid to be inoculated is dipped in the fungus liquid dipping tray (8), then the fungus liquid to be inoculated is inoculated to the culture plate of the culture fungus like stamping, and finally the purposes of evenly coating the fungus liquid on the surface of a culture medium of the culture plate and preventing the fungus liquid from producing a stamp point or scratches during inoculation from affecting the appearance of the culture plate are achieved. The invention has simple manufacture, strong operability, low cost and obvious effect.
Description
Technical Field
The invention relates to a seal type strain inoculation bag for a substance bacteriostasis experiment, and belongs to the technical field of biology.
Background
The bacterial inoculation methods commonly used in microbial detection mainly have five kinds: plate streaking, slant inoculation, puncture inoculation, liquid and semisolid inoculation, and spread inoculation. (1) plate scribing separation method: the method is characterized in that mixed microorganisms or different cells in the same microorganism group are inoculated on the surface of a flat culture medium by an inoculating loop, more independent single cells are obtained through zoning streak dilution, and are grown into single colonies after culture, and the single colonies are generally used as pure varieties of microorganisms to be separated. Sometimes, such single colonies are not all propagated from a single cell, and therefore, it is necessary to repeatedly isolate the single colony a plurality of times to obtain a pure strain. The principle is that the microorganism sample is diluted from point to line on the surface of the solid culture medium for a plurality of times to achieve the purpose of separation. For convenient streaking, the medium should not be too thin, and about 20m1 of medium should be poured per dish, the medium should be uniform in thickness, and the surface of the plate should be smooth. The scribing separation mainly comprises a partition scribing method and a continuous scribing method. The dividing and scribing method is also called as a four-division scribing method, in which a flat plate is divided into four areas. When the scribing is carried out, the flat plate is rotated for 60-70 degrees for scribing each time, and after the bacteria on the inoculating loop are burnt out each time when the angle is changed, the scribing is carried out at the place of the last scribing; another continuous streaking method is to continuously carry out wave streaking from one point at the edge of the plate until the other end of the plate, wherein the bacteria on the inoculating loop are not required to be burnt. The continuous scribing method is to burn the inoculating loop on the outer flame of the alcohol lamp until the inoculating loop is red, then to burn the inoculating rod fully, and finally to burn the inoculating loop intensively until the inoculating loop is red. The method comprises the steps of lightly shaking a test tube to be inoculated, holding the bottom side part of the test tube to be inoculated by a left-hand palm, holding an inoculating loop by a right-hand palm, pulling out a test tube plug by a right-hand little finger, extending the inoculating loop into the test tube near an alcohol lamp, later, inserting the inoculating loop into an inoculating liquid to be inoculated, dipping the inoculating liquid, taking the inoculating loop full of the inoculating loop, and withdrawing, burning the inoculating loop, covering and putting the inoculating loop back into a test tube rack. Or the inoculating loop is stretched into the flat plate through a gap with a dish cover which is slightly opened, the inoculating loop is cooled by contacting with a blank part at the edge of the flat plate, and then the bacterial colony to be separated and purified on the flat plate is directly taken out by the inoculating loop in a sterile operation. Opening the dish cover at a position close to the alcohol lamp by about 30 degrees, stretching the ring into the dish by the right hand, dibbling the strain at one position of the edge of the dish, lightly coating the strain on the edge of the agar culture medium, extracting the inoculating loop, covering the dish cover, burning redundant culture solution on the inoculating loop in flame, opening the dish cover by about 30 degrees to stretch into the inoculating loop, lightly contacting the inoculating solution after the inoculating loop is cooled, starting lightly sliding scribing on the surface of the dish, not embedding the inoculating loop into the culture medium to scratch the culture medium, enabling the lines to be parallel and dense, fully utilizing the surface area of the dish, taking care of not overlapping the front line and the rear line, and closing the dish cover after the scribing is completed. Firing the inoculating loop, and placing the inoculating loop on an inoculating rack after cooling. The culture dish is inverted and cultured in a temperature-adaptive incubator to prevent condensed water from dripping from the dish cover during the culture process, and the separated bacterial colony is dispersed. After culturing, the morphology of colonies growing along the streaks was observed on streak plates, and after microscopic examination, the slant was inoculated. The method of taking, inoculating and culturing by partition streaking is similar to the continuous streaking method. The plate is divided into four areas when the dividing and scribing method is used for separation, so the plate is also called a four-dividing and scribing method. The 4 th area is the main distribution area of single colony, so the streaking area should be maximum. In order to prevent the scribing line in the 4 th zone from contacting with the lines in the 1, 2 and 3 zones, the lines in the 4 th zone are parallel to the lines in the 1 zone, and the included angle between the lines in the zone and the lines in the zone is preferably kept about 120 degrees. When in inoculation, firstly, a small amount of bacteria are dipped in an inoculating loop to divide 3-5 parallel lines in a zone 1 of a flat plate, the inoculating loop is taken out, a dish cover is closed by the left hand, the flat plate is rotated for 60-70 degrees, redundant bacteria on the inoculating loop are burned by the right hand, the burned inoculating loop is cooled at the edge of the flat plate, and then the bacteria marked in the zone 1 are used as bacterial sources, and the zone 1 is used for carrying out parallel marking for the 2 nd time from the zone 2. After the 2 nd scribing, the plate is rotated for about 60-70 degrees, and the scribing is performed in the 3 rd and 4 th areas in turn. After the streaking is finished, the inoculating loop is burnt, the dish cover is closed, the culture is carried out in the same way, and single bacterial colony is observed in the streaking area. (2) The slant inoculation method is mainly used for pure culture of single colonies, preservation of strains or observation of certain characteristics of bacteria. The inclined plane inoculation method comprises the following operation steps: ① The left hand holds two test tubes flat, and the thumb presses the bottom of the test tube. The outer test tube is a strain test tube with fungus coating on the inclined surface, the inner test tube is a blank inclined surface to be connected, and the inclined surfaces of the two test tubes are upwards simultaneously. The tube plug was unscrewed with the right hand to allow easy withdrawal during inoculation. ② The inoculating loop is held by the right hand, as the writing brush is held, the end of the loop is firstly burnt to be red and sterilized on flame, and then the rest parts possibly extending into the test tube are sterilized by fire. ③ The upper ends of the two test tubes are aligned and close to the flame, the test tube plugs of the two test tubes are clamped and pulled out by the small finger and the palm center of the right hand, the test tube plugs are still clamped in the hands, and then the test tube ports slowly pass through the flame. Note that the test tube stopper should not be randomly placed on a table to be stained, and the test tube mouth should not burn too much to avoid burst. ④ The burnt inoculating loop is stretched into an outer strain test tube. The leading end of the loop is first brought into contact with the sterile moss medium to cool it if the operation is rapid, at which time the loop has not yet cooled completely. Then a certain amount of lawn is dipped by an inoculating loop according to the requirement, and the culture medium is not scraped. The inoculating loop stained with lawn is rapidly withdrawn from the test tube, and the inoculating loop is not made to touch the tube wall or tube orifice. ⑤ The inoculating loop with strain is quickly extended into the bottom of another inclined test tube to be connected, and the straight line or curve is gently drawn upwards, and the inoculating loop is determined according to the requirement, so that the surface of the culture medium is not scratched. ⑥ The inoculated inclined surface test tube port is subjected to flame again, and the bottom of the test tube plug is immediately plugged into the test tube after being subjected to flame. ⑦ The inoculating loop stained with lawn is burnt to red on flame for sterilization. Burning in inner flame to dry, and then burning in outer flame to avoid the flash heating of lawn, which can splash the thallus and cause pollution. ⑧ After the ring is put down, the test tube plug is screwed, and a label is attached to the position 2-3cm away from the test tube port above the outside of the test tube. (3) The puncture inoculation method is to sterilize with flame by using an inoculating needle, dip a small amount of strain, vertically penetrate into the center of a test tube solid culture medium to the bottom, then insert the inoculating needle for picking up bacterial colonies into the bottom of an agar inclined plane parallel to the tube wall, pull out the needle along the original inoculating line, burn a test tube plug, a test tube orifice and a test tube plug, and burn the residual bacterial on the inoculating needle on flame. The hand is stable, and the action is light and rapid. (4) Liquid inoculation methods in liquid and semisolid inoculation methods include inoculating the culture from an outside seed or from a liquid seed, both of which can be inoculated with an inoculating loop. However, in the case of a relatively large culture volume, liquid inoculation is preferably performed by pipetting, and aseptic manipulation is required. The test tube is held by the left hand, the cauterized inoculating loop is stretched into the strain tube by the right hand, after cooling, the strain liquid is taken as a loop and immediately transferred into the culture medium tube, the tube wall close to the liquid level is lightly ground, then the test tube is slightly inclined, and a small amount of liquid is dipped for blending, so that the strain liquid is mixed in the culture medium. The semi-solid culture medium inoculation method of the liquid and semi-solid inoculation method is to cool the burnt inoculating needle inserted into the strain tube, then to pick up a little bacterial liquid, immediately vertically insert the center of the semi-solid culture medium to the position close to the tube bottom, but not directly pierce the tube bottom, and then withdraw along the original path. The nozzle passes through flame, a cotton plug is plugged, and the inoculating needle is put down after being burnt and sterilized. The inoculated culture was cultured in an incubator at 37℃for 24 hours, and the observation result was taken out. (5) The coating inoculation method is to suck 0.1mL of bacterial suspension by using an aseptic pipette, drop the bacterial suspension onto a culture medium flat plate, hold an aseptic coating rod on the right hand, hold a culture dish on the left hand, open a gap on a dish cover by using a thumb, uniformly coat and spread bacterial liquid from the center of the flat plate to the periphery by holding the coating rod on the right hand beside flame and the surface of the culture dish flat plate, and avoid the situation that the bacterial liquid is directly pushed to the edge of the flat plate or the culture medium is scratched by excessive force. After inoculation, the plate was inverted into an incubator and observed in culture. From the above, each inoculation method requires the use of corresponding tools. Regarding the inoculation tool, yang Junyu et al invent a self-adhesive plant disease inoculation patch, the application number is CN200910074173.7, the utility model relates to a self-adhesive plant disease inoculation patch, belongs to an inoculation tool in the field of plant pathology research, and is widely used for inoculation experiments of plant pathogenic fungi and bacteria. The fixed-point quantitative inoculation device is simple in structure and convenient to manufacture, and solves the fixed-point quantitative inoculation problem of plant disease research. It is composed of two functional areas of sticking area and inoculating area. The sticking area is used for sticking to plant body to achieve fixed point, and has the functions of moisturizing and preventing rain wash. The inoculation area is provided with a fungus disk pot for placing fungus disks, so that the size of the fungus disks can be controlled, and quantitative inoculation is realized. The adhesive sticker plant disease inoculation paste can be manufactured into different shapes and different sizes according to different plant inoculation positions and the size of inoculation areas. Hua et al disclose a method of fungal inoculation, application number CN201610326970.X, and inoculation apparatus and culture medium for use in such a method. The fungus inoculation method of the utility model is to sterilize an inoculation tool with a tip end and a wooden or bamboo part at one end, transfer the inoculation tool to a culture medium with target fungus in the culture medium under aseptic environment, then transfer the inoculation tool to the culture medium with target fungus in the aseptic environment, then culture the culture medium, make the inoculation tool dye fungus with an inoculum composed of wooden or bamboo part, then take out the inoculation tool and pierce the tip end into an inoculation part of a host, so that the inoculation tool is infected with the wooden or bamboo inoculum fungus, thus realizing inoculation operation. The utility model has the characteristics of accuracy, convenience, time and labor saving and the like, and can be used for inoculating harder parts such as forests and the like. Huang Xun et al invent a novel bacterial inoculator with the application number of CN202121194148.5, and the utility model relates to a novel bacterial inoculator, belonging to the technical field of experimental instruments. The inoculating gun head is a cone, a spherical contact head for scribing culture is arranged at the tip end of the inoculating gun head, a groove for scraping thalli is formed in the middle lower part of the inoculating gun head, a jack is formed in the big head end of the cone, and one end of the operating handle is detachably inserted into the jack; the utility model has simple operation, and can ensure that the damage of the culture medium is avoided when bacteria are streaked for culture; the whole structure is single, the volume is small, the sterilization work of a large number of inoculation tools can be finished in advance, the enough sterile inoculation gun heads can be replaced when an inoculation test is carried out, and the improvement of inoculation efficiency is facilitated. The groove design is convenient for scraping a large amount of bacterial thalli at one time, and is beneficial to the rapid preparation of high-concentration bacterial suspension; the utility model can be repeatedly sterilized and used after cleaning, and can reduce the inoculation test cost. Currently, for whether certain substances have antibacterial activity, a circular paper sheet method is generally adopted for verification. The circular paper sheet method is to dip the water solution of the matter to be verified with circular filter paper sheet, culture the matter on the overgrown culture plate, and if the matter has antibacterial activity, after a period of time, no long bacterial transparent spots in circular ring form appear around the circular paper sheet on the culture plate, and the width of the circular ring indicates the antibacterial activity. During verification, antibacterial activity of different concentrations is often verified, a plurality of culture plates are required to be operated for each experiment, and microorganisms such as the same bacteria are cultured on the plurality of culture plates. According to the traditional method and using the traditional inoculation tool, a plurality of culture plates usually need a long time from preparation, sterilization and inoculation culture, so that in the experimental process of many people, the experiment is very tired, and is very tired, especially in the inoculation process, most time is spent, operators need a series of operations such as opening, coating, scribing, covering, sealing and the like, especially in the coating process, special care and high concentration are required, otherwise, the surface of the culture plates is marked with stamping points or scratches, therefore, how to complete the inoculation work rapidly and efficiently perfectly without uneven coating, the appearance of the culture plates is a great problem which is urgently needed to be solved when the surface of the culture plates is not marked with stamping points or scratches and influences on the observation, therefore, the thin-wall silicone capsule with the fungus liquid capsule membrane process is used for dipping trace fungus liquid to be inoculated in a fungus liquid dipping disc, then the fungus liquid to be inoculated is inoculated on a culture plate for culturing fungus like stamping, and finally the purposes of uniformly coating the fungus liquid on the surface of a culture medium of the culture plate and preventing the appearance of a stamping point or scratch from affecting the appearance of the culture plate during observation are achieved.
Disclosure of Invention
In order to overcome the problem that the surface of a culture plate is free from stamping points or scratches and influences the appearance of the culture plate when the inoculation work is completed rapidly and efficiently and perfectly, the invention provides a seal type strain inoculation bag for a substance bacteriostasis experiment.
The technical scheme adopted for solving the technical problems is as follows:
The invention relates to a seal type strain inoculation bag for a substance bacteriostasis experiment, which consists of a fungus dipping liquid bag 1 and a fungus dipping liquid disc 8, and is characterized in that: the fungus dipping liquid bag 1 is an inoculation bag which is used for dipping fungus liquid in a fungus dipping liquid tray 8 and then inoculating the fungus liquid to a culture plate, and consists of a bag fixing plate 2, a handle 3, a water adding pipe 4, a handle cover 5, a handle cap 6, a connecting bolt 7, a fungus dipping liquid bag film 13 and water 14, wherein the fungus dipping liquid bag film 13 is a film which is used for holding water at the lowest end of the fungus dipping liquid bag 1 and is silica gel, the thickness is 0.1-0.2 mm, the periphery of the fungus dipping liquid bag film 13 is sealed and fixed on the periphery of the bag fixing plate 2, and the fungus dipping liquid bag film 13 consists of a fungus dipping liquid bag film body 15 and a fungus dipping liquid bag film protrusion 16; the fungus dipping liquid capsule film body 15 is a main body of the fungus dipping liquid capsule film 13, and in a natural state, the thickness of the fungus dipping liquid capsule film body 15 is 0.09-0.18 mm, and when the fungus dipping liquid capsule film body 15 is acted by the gravity of water 14, the thickness of the fungus dipping liquid capsule film body 15 is thinned; the fungus dipping liquid capsule membrane process 16 is a hexagonal bulge distributed on the outer surface of the fungus dipping liquid capsule membrane body 15, the side length of the hexagonal fungus dipping liquid capsule membrane process 16 is 1-5 microns, the thickness of the fungus dipping liquid capsule membrane process 16 is 0.01-0.02 mm, and the distance between two adjacent fungus dipping liquid capsule membrane processes 16 is 1-5 microns; after the water 14 is poured into the fungus dipping liquid bag 1, the fungus dipping liquid bag film 13 sags by 1-2 cm under the action of gravity; the bag fixing plate 2 is a circular plate for fixing the fungus dipping liquid bag film 13 and is made of rubber, plastic, copper, stainless steel or aluminum alloy, the thickness of the bag fixing plate 2 is 2-5 mm, the diameter of the bag fixing plate is 2-10 cm, and the upper surface of the bag fixing plate is fixedly arranged at the lower end of the handle 3 and is connected with the handle 3 into a whole; the handle 3 is a holding structure above the bag fixing plate 2, the lower end is connected with the bag fixing plate 2, the upper end is free, the outer surface of the upper end is provided with a rotary wire, the inner surface of the handle cap 6 is also provided with a rotary wire, and the rotary wire on the outer surface of the upper end of the handle 3 and the rotary wire on the inner surface of the handle cap 6 form a connecting bolt 7 together; the water adding pipe 4 is a central pipeline of the handle 3, the pipe diameter of the upper end of the water adding pipe 4 is thick and is in a funnel shape, the diameter of a cross section circle of the upper end of the funnel is 0.5-1 cm, the diameter of a cross section circle of the lower end of the funnel is 0.2-0.5 cm, the height of the funnel is 1-2 cm, the pipe diameter of the lower part of the water adding pipe 4 is thin and is in a cylinder shape, the diameter of a cross section circle of the cylinder is 0.2-0.5 cm, and the lower end of the water adding pipe 4 is communicated with an inner cavity which is formed by the bacteria dipping liquid capsule 13 and the solid capsule plate 2 and used for containing water 14; the handle cover 5 is made of rubber, plastic, copper, stainless steel or aluminum alloy, the upper half part of the handle cover 5 is cylindrical, the diameter of the cylinder is 1-3 cm, and the height of the cylinder is 0.5-1 cm; the lower half part of the handle cover 5 is a handle cap 6, the handle cap 6 is cylindrical, and the inner surface is provided with a rotary wire meshed with the rotary wire on the outer surface of the upper end of the handle 3.
The fungus dipping liquid tray 8 is a concave tray for containing fungus liquid to be inoculated for dipping the fungus liquid bag 1 to dip the fungus liquid, is ceramic, vitreous, stainless steel, copper or aluminum alloy, and consists of a fungus dipping liquid tray cavity 9, a fungus dipping liquid tray wall 10, a tray handle 11 and a base 12; the fungus dipping liquid tray cavity 9 is a downward concave cavity surrounded by a fungus dipping liquid tray wall 10 at the top of the fungus dipping liquid tray 8; the fungus dipping liquid tray wall 10 is positioned at the top of the fungus dipping liquid tray 8 and is in a spherical shape with the thickness of 1-5 mm; the diameter of the circular opening at the upper edge of the fungus dipping liquid tray wall 10 is 15-20 cm, the maximum depth of the fungus dipping liquid tray wall 10 is 1 cm, and the inner surface of the fungus dipping liquid tray wall 10 is smooth; the tray handle 11 is a connecting part with the upper end connected to the center of the outer surface of the bottom of the fungus dipping tray wall 10 and the lower end connected to the center of the upper surface of the base 12, the height of the tray handle 11 is 3-5 cm, the cross section is round, the diameter of the cross section of the upper end of the tray handle 11 is 0.5-1 cm, and the diameter of the cross section of the lower end is 10-15 cm; the base 12 is a structural part at the lower part of the bacteria dipping liquid tray 8, and is cylindrical, and the diameter of the cylindrical base 12 is 10-15 cm, and the height is 1-2 cm.
The stamp type strain inoculation bag for the material bacteriostasis experiment has the beneficial effects that the stamp type strain inoculation bag for the material bacteriostasis experiment utilizes the thin-wall silicone bag with the fungus liquid bag film dipping process on the outer wall of the water, dips trace fungus liquid to be inoculated in the fungus liquid dipping tray, and inoculates the fungus liquid to be inoculated on a culture plate for culturing fungus like stamping, so that the purposes of uniformly coating the fungus liquid on the surface of a culture medium of the culture plate and preventing the appearance of the culture plate from being influenced by stamping points or scratches during inoculation are finally achieved. The invention has simple manufacture, strong operability, low cost and obvious effect.
Drawings
The invention is further described below with reference to the accompanying drawings.
Fig. 1 is a schematic diagram of the whole structure of a fungus dipping liquid bag of a seal type fungus inoculation bag for a material bacteriostasis experiment.
FIG. 2 is a schematic diagram of the whole structure of a fungus dipping liquid tray of a seal type fungus inoculation bag for a material bacteriostasis experiment.
FIG. 3 is a schematic diagram of the cross-sectional structure of the fungus dipping liquid capsule membrane of the stamp type fungus inoculation capsule for the material bacteriostasis experiment.
In the figure, 1 part of fungus dipping liquid bag, 2 parts of bag fixing plate, 3 parts of handle, 4 parts of water adding pipe, 5 parts of handle cover, 6 parts of handle cap, 7 parts of connecting bolt, 8 parts of fungus dipping liquid tray, 9 parts of fungus dipping liquid tray cavity, 10 parts of fungus dipping liquid tray wall, 11 parts of tray handle, 12 parts of base, 13 parts of fungus dipping liquid bag film, 14 parts of water, 15 parts of fungus dipping liquid bag film body and 16 parts of fungus dipping liquid bag film.
Detailed Description
Embodiment one:
As shown in the figure, the stamp type strain inoculation bag for the substance bacteriostasis experiment consists of a fungus dipping liquid bag 1, a bag fixing plate 2, a handle 3, a water adding pipe 4, a handle cover 5, a handle cap 6, a connecting bolt 7, a fungus dipping liquid tray 8, a fungus dipping liquid tray cavity 9, a fungus dipping liquid tray wall 10, a tray handle 11, a base 12, a fungus dipping liquid bag film 13, water 14, a fungus dipping liquid bag film body 15 and a fungus dipping liquid bag film protrusion 16. The fungus dipping liquid bag 1 is an inoculation bag which is used for dipping fungus liquid in a fungus dipping liquid tray 8 and then inoculating the fungus liquid to a culture plate, and consists of a bag fixing plate 2, a handle 3, a water adding pipe 4, a handle cover 5, a handle cap 6, a connecting bolt 7, a fungus dipping liquid bag film 13 and water 14, when the fungus dipping liquid bag 1 is used, an operator holds the handle, the fungus dipping liquid bag film 13 of the fungus dipping liquid bag 1 is placed under a pier 2-5 of a fungus dipping liquid tray cavity 9 which is dripped with fungus liquid to be inoculated, then the fungus dipping liquid to be inoculated is picked up, a small amount of fungus liquid to be inoculated can be adhered to the outer surface of the fungus dipping liquid bag film 13, then the fungus dipping liquid is gently placed like a stamp seal on the culture plate to be cultivated, after the fungus liquid to be inoculated is picked up, the fungus liquid to be inoculated can be coated on the surface of the culture plate relatively uniformly, thereby avoiding the repeated rolling of the ball and other inoculation modes or the scratch possibly caused to the surface of the culture plate in the inoculation process of the inoculation loop, leading the bacteria on the surface of the inoculated culture plate to uniformly grow, providing uniform bacterial colonies for the bacteria inhibition paper sheets, and improving the display definition of the bacteria inhibition effect; the fungus dipping liquid capsule membrane 13 is a thin capsule membrane for containing water at the lowest end of the fungus dipping liquid capsule 1, is a silica gel, and has a thickness of 0.1-0.2 mm, the periphery of the fungus dipping liquid capsule membrane 13 is sealed and fixed at the periphery of the capsule fixing plate 2, and the fungus dipping liquid capsule membrane 13 consists of a fungus dipping liquid capsule membrane body 15 and fungus dipping liquid capsule membrane protrusions 16; the fungus dipping liquid capsule film body 15 is a main body of the fungus dipping liquid capsule film 13, and the fungus dipping liquid capsule film body 15 has a thickness of 0.09-0.18 mm in a natural state, and becomes thinner when being acted by the gravity of water 14; the fungus dipping liquid capsule membrane process 16 is a hexagonal bulge distributed on the outer surface of the fungus dipping liquid capsule membrane body 15, the side length of the hexagonal fungus dipping liquid capsule membrane process 16 is 1-5 micrometers, the thickness of the fungus dipping liquid capsule membrane process 16 is 0.01-0.02 millimeter, the distance between two adjacent fungus dipping liquid capsule membrane processes 16 is 1-5 micrometers, and the fungus dipping liquid capsule membrane process 16 is arranged to enable the outer surface of the fungus dipping liquid capsule membrane 13 to be rough so as to better dip fungus liquid to be inoculated; after the water 14 is poured into the fungus dipping liquid bag 1, the fungus dipping liquid bag film 13 sags by 1-2 cm under the action of gravity, so that when fungus dipping liquid is performed, the fungus dipping liquid bag film 13 can be tightly pressed with the fungus dipping liquid tray wall 10, fungus liquid to be inoculated is uniformly filled in a gap between the outer surface of the fungus dipping liquid bag film body 15 and the inner surface of the fungus dipping liquid tray wall 10, the consumption of the fungus liquid is reduced, and a very small drop of fungus liquid can be inoculated to a plurality of culture plates; the bag fixing plate 2 is a circular plate for fixing the fungus dipping liquid bag film 13, is made of rubber, plastic, copper, stainless steel or aluminum alloy, the thickness of the bag fixing plate 2 is 2-5 mm, the diameter of the bag fixing plate is 2-10 cm, the upper surface of the bag fixing plate is fixedly arranged at the lower end of the handle 3 and is connected with the handle 3 into a whole, when the fungus dipping liquid bag film 13 is fixed, the periphery of the fungus dipping liquid bag film 13 is adhered to the periphery of the bag fixing plate 2 by glue, and then the fungus dipping liquid bag film 13 can be fixed on the bag fixing plate 2 in a sealing manner by using iron wires or iron sheets; the handle 3 is a holding structure above the bag fixing plate 2, the lower end is connected with the bag fixing plate 2, the upper end is free, the outer surface of the upper end is provided with a rotary wire, the inner surface of the handle cap 6 is also provided with a rotary wire, the rotary wire on the outer surface of the upper end of the handle 3 and the rotary wire on the inner surface of the handle cap 6 form a connecting bolt 7 together, and the connecting bolt 7 is used for being connected with the rotary wire on the inner surface of the handle cap 6 in a rotary way to prevent the poured water 14 from overflowing; the water adding pipe 4 is a central pipeline of the handle 3, the pipe diameter of the upper end of the water adding pipe 4 is thick and is in a funnel shape, the diameter of the cross section circle of the upper end of the funnel is 0.5-1 cm, the diameter of the cross section circle of the lower end of the funnel is 0.2-0.5 cm, the height of the funnel is 1-2 cm, the pipe diameter of the lower part of the water adding pipe 4 is thin and is in a cylinder shape, the diameter of the cross section circle of the cylinder is 0.2-0.5 cm, and the lower end of the water adding pipe 4 is communicated with the inner cavity of the water 14 formed by the fungus dipping liquid capsule 13 and the solid capsule plate 2 and is used for adding the water 14 into the inner cavity of the water 14 formed by the fungus dipping liquid capsule 13 and the solid capsule plate 2; the handle cover 5 is a structure in which the upper end of the handle 3 is sealed after water 14 is added into an inner cavity formed by the bacteria dipping liquid capsule membrane 13 and the capsule fixing plate 2 and used for containing water 14, the upper half part of the handle cover 5 is cylindrical, the diameter of the cylinder is 1-3 cm, and the height is 0.5-1 cm; the lower half part of the handle cover 5 is a handle cap 6, the handle cap 6 is cylindrical, the inner surface is provided with a rotary wire meshed with the rotary wire at the outer surface of the upper end of the handle 3, and after the handle cover 5 is tightly screwed at the upper end of the handle 3, the water adding pipe 4 can be closed to prevent water 14 from overflowing. The fungus dipping liquid tray 8 is a concave tray for containing fungus liquid to be inoculated for dipping the fungus liquid bag 1 to dip the fungus liquid, is ceramic, vitreous, stainless steel, copper or aluminum alloy, and consists of a fungus dipping liquid tray cavity 9, a fungus dipping liquid tray wall 10, a tray handle 11 and a base 12; the fungus dipping liquid tray cavity 9 is a downward concave cavity surrounded by a fungus dipping liquid tray wall 10 at the top of the fungus dipping liquid tray 8; the fungus dipping liquid tray wall 10 is positioned at the top of the fungus dipping liquid tray 8 and is in a spherical shape with the thickness of 1-5 mm; the diameter of the circular opening at the upper edge of the fungus dipping liquid tray wall 10 is 15-20 cm, the maximum depth of the fungus dipping liquid tray wall 10 is 1 cm, and the inner surface of the fungus dipping liquid tray wall 10 is smooth; the tray handle 11 is a connecting part with the upper end connected to the center of the outer surface of the bottom of the fungus dipping tray wall 10 and the lower end connected to the center of the upper surface of the base 12, and is used for picking up or moving the fungus dipping tray 8, the height of the tray handle 11 is 3-5 cm, the cross section is circular, the diameter of the cross section of the upper end of the tray handle 11 is 0.5-1 cm, and the diameter of the cross section of the lower end is 10-15 cm; the base 12 is a structural part at the lower part of the bacteria dipping liquid tray 8 and is cylindrical, the diameter of the cylindrical base 12 is 10-15 cm, and the height is 1-2 cm; when the device is used, the bacterium dipping liquid tray 8 is sterilized in advance, then the bacterium dipping liquid tray is placed in an ultra-clean workbench, the bacterium to be inoculated is sucked by a pipette, the bacterium dipping liquid tray is blown into the bacterium dipping liquid tray cavity 9, if a plurality of inoculated culture plates are used, the bacterium dipping liquid tray 8 can be held by one hand, the bacterium dipping liquid bag 1 can be quickly inoculated onto the culture plates like stamping, and the inoculation work can be quickly and efficiently completed when a plurality of culture plates are needed for culturing the bacteria due to no need of rolling balls or line drawing.
The foregoing has shown and described the basic principles and main features of the present invention and the advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the foregoing embodiments, which have been described in the foregoing embodiments and description merely illustrates the principles of the invention, and that various changes and modifications may be effected therein without departing from the spirit and scope of the invention as defined in the appended claims and their equivalents.
Claims (2)
1. The seal type strain inoculation bag for the material bacteriostasis experiment consists of a fungus dipping liquid bag (1) and a fungus dipping liquid disc (8), and is characterized in that: the fungus dipping liquid bag (1) is an inoculation bag which is used for dipping fungus liquid in a fungus dipping liquid tray (8) and then inoculating the fungus liquid to a culture plate, and consists of a bag fixing plate (2), a handle (3), a water adding pipe (4), a handle cover (5), a handle cap (6), a connecting bolt (7), a fungus dipping liquid bag film (13) and water (14), wherein the fungus dipping liquid bag film (13) is a film of the lowest end of the fungus dipping liquid bag (1) and is silica gel, the thickness is 0.1-0.2 mm, the periphery of the fungus dipping liquid bag film (13) is sealed and fixed on the periphery of the bag fixing plate (2), and the fungus dipping liquid bag film (13) consists of a fungus dipping liquid bag film body (15) and a fungus dipping liquid bag film protrusion (16); the fungus dipping liquid capsule film body (15) is a main body of the fungus dipping liquid capsule film (13), the thickness of the fungus dipping liquid capsule film body (15) is 0.09-0.18 mm in a natural state, and the thickness of the fungus dipping liquid capsule film body (15) is thinned when the fungus dipping liquid capsule film body is acted by the gravity of water (14); the fungus dipping liquid capsule membrane protrusions (16) are hexagonal bulges distributed on the outer surface of the fungus dipping liquid capsule membrane body (15), the side length of each hexagonal fungus dipping liquid capsule membrane protrusion (16) is 1-5 microns, the thickness of each fungus dipping liquid capsule membrane protrusion (16) is 0.01-0.02 mm, and the distance between two adjacent fungus dipping liquid capsule membrane protrusions (16) is 1-5 microns; after the water (14) is poured into the fungus dipping liquid bag (1), the fungus dipping liquid bag film (13) sags by 1-2 cm under the action of gravity; the bag fixing plate (2) is a circular plate for fixing the fungus dipping liquid bag film (13) and is made of rubber, plastic, copper, stainless steel or aluminum alloy, the thickness of the bag fixing plate (2) is 2-5 mm, the diameter of the bag fixing plate is 2-10 cm, and the upper surface of the bag fixing plate is fixedly arranged at the lower end of the handle (3) and is connected with the handle (3) into a whole; the handle (3) is a holding structure above the bag fixing plate (2), the lower end of the handle is connected with the bag fixing plate (2), the upper end of the handle is free, the outer surface of the upper end of the handle is provided with a rotary wire, the inner surface of the handle cap (6) is also provided with a rotary wire, and the rotary wire on the outer surface of the upper end of the handle (3) and the rotary wire on the inner surface of the handle cap (6) form a connecting bolt (7) together; the water adding pipe (4) is a central pipeline of the handle (3), the pipe diameter of the upper end of the water adding pipe (4) is thick, the upper end of the water adding pipe (4) is in a funnel shape, the diameter of a cross section circle of the upper end of the funnel is 0.5-1 cm, the diameter of a cross section circle of the lower end of the funnel is 0.2-0.5 cm, the height of the funnel is 1-2 cm, the pipe diameter of the lower part of the water adding pipe (4) is thin, the diameter of the cross section circle of the cylinder is in a cylinder shape, and the lower end of the water adding pipe (4) is communicated with an inner cavity for containing water (14) formed by the fungus liquid sac membrane (13) and the solid sac plate (2); the handle cover (5) is made of rubber, plastic, copper, stainless steel or aluminum alloy, the upper half part of the handle cover (5) is cylindrical, the diameter of the cylinder is 1-3 cm, and the height of the cylinder is 0.5-1 cm; the lower half part of the handle cover (5) is a handle cap (6), the handle cap (6) is cylindrical, and the inner surface is provided with a spinning thread meshed with the spinning thread on the outer surface of the upper end of the handle (3).
2. The stamp-type strain inoculation bag for substance bacteriostasis experiments according to claim 1, wherein: the fungus dipping liquid tray (8) is a concave tray for containing fungus liquid to be inoculated for dipping fungus liquid bags (1) to dip fungus liquid, is ceramic, vitreous, stainless steel, copper or aluminum alloy, and consists of a fungus dipping liquid tray cavity (9), fungus dipping liquid tray walls (10), tray handles (11) and a base (12); the fungus dipping liquid tray cavity (9) is a downward concave cavity surrounded by a fungus dipping liquid tray wall (10) at the top of the fungus dipping liquid tray (8); the wall (10) of the fungus dipping liquid tray is positioned at the top of the fungus dipping liquid tray (8) and is in a spherical shape with the thickness of 1-5 mm; the diameter of the circular opening at the upper edge of the fungus dipping liquid tray wall (10) is 15-20 cm, the maximum depth of the fungus dipping liquid tray wall (10) is 1 cm, and the inner surface of the fungus dipping liquid tray wall (10) is smooth; the tray handle (11) is a connecting part with the upper end connected to the center of the outer surface of the bottom of the fungus dipping tray wall (10) and the lower end connected to the center of the upper surface of the base (12), the height of the tray handle (11) is 3-5 cm, the cross section is round, the diameter of the cross section of the upper end of the tray handle (11) is 0.5-1 cm, and the diameter of the cross section of the lower end is 10-15 cm; the base (12) is a structural part at the lower part of the bacteria dipping liquid tray (8) and is cylindrical, the diameter of the cylindrical base (12) is 10-15 cm, and the height is 1-2 cm.
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