CN220887531U - Skin microorganism cultivates detection device - Google Patents

Skin microorganism cultivates detection device Download PDF

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Publication number
CN220887531U
CN220887531U CN202322775443.5U CN202322775443U CN220887531U CN 220887531 U CN220887531 U CN 220887531U CN 202322775443 U CN202322775443 U CN 202322775443U CN 220887531 U CN220887531 U CN 220887531U
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liquid injection
sampling
detection
liquid
limiting
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CN202322775443.5U
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王乐阳
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Wuhan University WHU
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Wuhan University WHU
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Abstract

A skin microorganism culture detection device comprises a culture dish, a sampling cover and a pipetting cylinder; the inner side surface of the culture dish is uniformly provided with a plurality of limiting tables at intervals, the inner side of each limiting table is provided with a detection box, and the surface of each detection box is provided with a plurality of detection grooves in an array manner; the top surface of the sampling cover is provided with an operating handle, the bottom surface of the operating handle is detachably provided with a sampling disc, and sampling heads are arranged on the bottom surface of the sampling disc in an array manner; the liquid transferring cylinder is detachably arranged in the operating handle; the middle part of detection box is provided with the notes cistern, be provided with a plurality of runners in the detection box, the inner bottom of pipetting cylinder is provided with annotates the liquid passageway, be provided with the piston push rod in the pipetting cylinder in a supporting way. The utility model can sample, culture and identify the microorganisms on the face of the subject, is convenient for culturing the microorganisms or protecting the microorganisms before gene sequencing, is convenient and quick to use, simplifies the operation process, and can intuitively detect the whole state of skin microorganisms in a region.

Description

Skin microorganism cultivates detection device
Technical Field
The utility model relates to the technical field of microorganism inspection equipment, in particular to a skin microorganism culture detection device.
Background
Human skin possesses 19 and over 1000 microorganisms, including bacteria, fungi and viruses, which are located in the epidermis and the pilosebaceous glands. The skin microflora is closely related to skin health: firstly, the cosmetic composition plays an important role in the skin defense system, secondly, the cosmetic composition is related to various skin diseases including acne, and thirdly, different types of cosmetics have a remarkable influence on the skin microflora.
Microbial detection generally has three steps: sampling, culturing and identification of microorganisms currently there are generally three methods for microbiological detection: after culturing the sampled microorganisms, judging dominant microorganism groups at the sampling part according to the properties (size, shape, color, glossiness, transparency, texture, swelling state, edge characteristics and the like) of the colony; culturing the sampled microorganism and then carrying out gene sequencing; the sampled microorganism is directly subjected to gene sequencing without culturing, and the operation difficulty, the detection range and the accuracy of the detection result are different.
The current microorganism sampling tool mainly comprises an inoculating needle and an inoculating loop, and can pick microorganisms in a target area for inoculation. Picking aerobic microorganisms by an inoculating loop, and inoculating on a culture medium; the anaerobic microorganisms are inoculated by an inoculating needle puncture method. However, the above method has the following drawbacks: firstly, when skin microorganisms are collected, only microorganisms in a micro area can be selected each time, and microorganisms in the whole skin in a certain area cannot be effectively sampled; secondly, when an inoculating loop is used, the inoculating loop is easy to be inadequately inoculated or scratch the culture medium; thirdly, when the anaerobic microorganisms are inoculated by adopting an inoculating needle puncture method, puncture angle and depth deviation are easy to cause.
At present, whether it is scientific research or cosmetic and personal care markets, there is an urgent need for an integrated device that can effectively sample and culture microorganisms in a certain area of skin, and that has sampling tools, culture tools, and identified pretreatment to improve detection efficiency.
Disclosure of utility model
In order to solve the problems, the utility model provides a skin microorganism culture detection device, which comprises the following specific technical scheme:
A skin microbial cultivation detection device, comprising:
The culture dish is characterized in that a plurality of limiting tables are uniformly arranged on the inner side surface of the culture dish at intervals, a detection box attached to the inner side of each limiting table is detachably placed in the culture dish, and a plurality of detection grooves are formed in the surface of each detection box in an array mode;
the sampling cover is provided with an operating handle on the top surface and a sampling disc on the bottom surface in a detachable way, sampling heads which can be placed in the detection groove are arranged on the bottom surface array of the sampling disc, the sampling disc can be covered in the culture dish, and the periphery of the bottom surface of the sampling disc can be placed on the limiting table;
A pipetting cylinder detachably placed in the handle for quantitatively injecting a desired liquid into the culture dish or the detection tank;
The middle part of the detection box is provided with a liquid injection groove matched with the liquid injection barrel, a plurality of flow passages which enable the liquid injection groove to be respectively communicated with the inner top sides of the detection grooves are arranged in the detection box, the inner bottom of the liquid injection barrel is provided with a liquid injection channel which can be communicated with the liquid injection groove, and a piston push rod which enables liquid in the liquid injection barrel to be injected into the liquid injection groove from the liquid injection channel is arranged in the liquid injection barrel in a matched mode.
Further, the sampling head may be configured as a first sampling head immersed in the culture medium or a second sampling head in contact with the surface of the culture medium.
Further, when the target cultured microorganism contains mold, the interval between adjacent sampling heads is 3 to 5cm.
Further, a plurality of limiting pieces used for positioning and placing are correspondingly arranged between the limiting table and the detection box and between the culture dish and the sampling plate respectively, and the plurality of limiting pieces divide the peripheral ring into three equal parts at least.
Further, the locating part includes spacing groove and rather than assorted spacing post, the spacing groove is all seted up to the lateral surface of detection box and sampling dish, the medial surface of spacing platform and culture dish all sets up spacing post.
Further, annotate the liquid passageway and link up in the interior bottom of liquid transfer cylinder, annotate the top of liquid passageway and set up the roof that has the through-hole, annotate the interior movable cover of liquid passageway and be equipped with the sealing rod, the top of sealing rod upwards passes the through-hole and extends to the outside of roof, and the top of sealing rod is provided with the sealing block that can seal the through-hole, and the cover is equipped with the pressure spring on the sealing rod that is located annotating the liquid passageway, the bottom of pressure spring is connected in the bottom of sealing rod, and its top is connected in the bottom surface of roof, the initial position of pressure spring makes the sealing block seal on annotating the liquid passageway.
Further, a push rod which can be inserted into the liquid injection channel is arranged in the liquid injection groove, the push rod is communicated with each flow channel, and a liquid injection hole communicated with the inside of the push rod is formed in the top side surface of the push rod.
Further, a sealing ring matched with the liquid injection channel is arranged on the pushing rod close to the liquid injection hole.
Further, the pipetting cylinder with the piston push rod can be placed in the operating handle, and the rubber sleeve plug is detachably arranged at the top of the operating handle.
The beneficial effects are that:
The utility model can sample, culture and identify the microorganism on the face of the subject, is convenient for culturing the microorganism or protecting the microorganism before gene sequencing, is convenient and quick to use, simplifies the operation process, and can intuitively detect the whole state of skin microorganism in a region; multiple groups of sampling heads can be arranged to adapt to different culture modes of different microorganisms, so that the use is flexible and the applicability is strong; the protective liquid can be injected into the detection groove at one time, so that the operation efficiency is improved.
Drawings
Fig. 1 is an overall schematic of the present utility model.
FIG. 2 is a schematic view of the cassette of the present utility model placed in a petri dish.
FIG. 3 is a schematic view of a pipetting cylinder and piston pusher according to the utility model.
Fig. 4 is an enlarged schematic view of the liquid injection groove at a in fig. 2 according to the present utility model.
In the figure: 1a culture dish and 11a limiting table; 2, a detection box, a liquid injection groove, a push rod, a liquid injection hole, a sealing ring and a detection groove, wherein the liquid injection groove is formed in the detection box, the push rod is arranged in the detection groove, and the detection groove is formed in the detection groove; 3, a sampling cover, a 31 operation handle, a 32 rubber sleeve plug and a 33 sampling head; 4 liquid transferring cylinders, 41 liquid injecting channels, 42 sealing rods, 43 sealing blocks and 44 compression springs; 5 a piston push rod; 6, a limit groove; and 7, limiting the column.
Detailed Description
The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application. It will be apparent that the described embodiments are only some, but not all, embodiments of the application. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to fall within the scope of the application.
In the description of the present application, the meaning of "a plurality" is two or more, unless explicitly defined otherwise. In the description of the present application, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically connected, electrically connected or can be communicated with each other; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art according to the specific circumstances.
As shown in fig. 1 to 4, a skin microorganism culture detection device comprises a culture dish 1, a sampling cover 3 and a pipetting cylinder 4; a culture medium can be injected into the culture dish 1, and the culture dish is suitable for culturing microorganisms; the sampling cover 3 is used for installing different sampling tools for sampling microorganisms of the facial skin of a subject; the pipetting cylinder 4 is used for quantitatively measuring liquid reagents such as culture medium or protective liquid.
As shown in fig. 1 and 2, the inner side surface of the culture dish 1 is uniformly provided with a plurality of limiting tables 11 at intervals, the culture dish 1 is detachably provided with a detection box 2 attached to the inner side of the limiting tables 11, and the surface array of the detection box 2 is provided with a plurality of detection grooves 25.
When the culture dish 1 is used alone, a quantitative culture medium can be directly poured into the pipetting cylinder 4 and used for culturing anaerobic or aerobic microorganisms so as to detect the growth speed of different types of microorganism colonies and the like. The detection box 2 is used for non-cultured microorganisms, the detection box 2 is placed in the culture dish 1, and nucleic acid protection liquid or 16sRNA protection liquid and the like are injected into the detection groove 25, so that the subsequent microorganism gene sequencing is facilitated.
As shown in fig. 3, the top surface of the sampling cover 3 is provided with an operating handle 31, the bottom surface thereof is detachably provided with a sampling tray, the bottom surface array of the sampling tray is provided with sampling heads 33 which can be placed in the detection groove 25, the sampling tray can be covered in the culture dish 1, and the periphery of the bottom surface of the sampling tray can be placed on the limiting table 11.
In one embodiment, the sampling plate is screwed or snapped onto the sampling cap 3 to facilitate the removal and replacement of the different sampling heads 33.
The outer diameter of the sampling plate is slightly smaller than the inner diameter of the culture dish 1, so that the sampling plate can be well placed in the culture dish 1 and placed on the limiting table 11, and the sampling head 33 is suspended in the culture dish 1 and can be in contact with the culture medium.
After the detection box 2 is placed in the culture dish 1, the top surface of the detection box 2 is flush with the top surface of the limiting table 11, and when the sampling disc is placed in the culture dish 1, the bottom end of the sampling head 33 can be immersed in the liquid of the corresponding detection groove 25.
As shown in fig. 1 and 3, the pipetting cylinder 4 is detachably mounted in the lever 31 for quantitatively pouring a desired liquid into the culture dish 1 or the detection tank 25.
Wherein, the outer wall of the pipetting cylinder 4 is provided with graduations for accurately measuring the measured liquid volume.
In one embodiment, the middle part of the detection box 2 is provided with a liquid injection groove 21 matched with the liquid injection groove 4, the detection box 2 is internally provided with a plurality of flow passages which enable the liquid injection groove 21 to be respectively communicated with the inner top sides of the detection grooves 25, the inner bottom part of the liquid injection groove 4 is provided with a liquid injection channel 41 which can be communicated with the liquid injection groove 21, and the liquid injection groove 4 is internally provided with a piston push rod 5 which can enable liquid in the liquid injection groove to be pressed into the liquid injection groove 21 from the liquid injection channel 41 in a matched manner, so that the liquid can be injected into the detection grooves 25 at the same time, and the working efficiency is improved.
The flow channels are provided as thin as possible to avoid waste due to a large amount of liquid remaining therein, and of course, the liquid remaining in the flow channels may be blown into the detection groove 25 by the piston rod 5. The liquid injection amount in the detection tank 25 is not too large, so that the liquid in the detection tank 25 is prevented from overflowing and flowing back from a flow channel arranged in the detection tank 25 after the bottom end of the sampling head 33 is immersed in the detection tank 25.
As shown in fig. 1, the sampling head 33 may be provided as a first sampling head 33 immersed in the culture medium or a second sampling head 33 in contact with the surface of the culture medium.
In one embodiment, the first sampling head 33 is elongated and has a relatively sharp bottom end (not shown) and a length such that the bottom end thereof is completely inserted into the culture medium in the test dish to culture anaerobic microorganisms; the second sampling head 33 is a short and blunt inverted triangle, the included angle of the bottom end is 45-60 degrees, and the length is such that the bottom end just contacts with the surface of the culture medium to culture aerobic microorganisms.
The density gradient of the sampling head 33 can be set according to the abundance of the microorganisms on the skin to be detected, so that it can be used to detect the skin with different abundance of the microorganisms. According to the specific conditions of the physiological state of the skin of a subject, the target culture time, the target culture microorganisms and the types of culture media matched with the target culture microorganisms, colony growth speeds of different types of microorganisms and the like, the density gradient of the sampling head 33 also has a larger interval range; illustratively, when the microorganism of interest comprises mold, the spacing between adjacent sampling heads 33 is 3-5 cm.
It should be noted that the monitoring cartridge may also be provided with a plurality of sets, which are correspondingly configured according to the number of sampling heads 33.
As shown in fig. 2 and 3, a plurality of limiting pieces for positioning and placing are correspondingly arranged between the limiting table 11 and the detection box 2 and between the culture dish 1 and the sampling plate respectively, and the plurality of limiting pieces divide the circumference into at least three equal parts.
Wherein, the spacing piece of everywhere distinguishes through different colours, and the microorganism of sample can accurately correspond to the zoning in culture dish 1 and the detection box 2 according to the colour on the sampling dish to can distinguish the microorganism of the different regions of corresponding to the subject's face fast when cultivateing or sequencing.
In one embodiment, the limiting piece comprises a limiting groove 6 and a limiting column 7 matched with the limiting groove, the limiting groove 6 is formed in the outer side faces of the detection box 2 and the sampling plate, and the limiting column 7 is arranged on the inner side faces of the limiting table 11 and the culture dish 1.
The limiting table 11, the detecting box 2, the culture dish 1 and the sampling plate can be made of plastic or glass, wherein the limiting groove 6 and the limiting column 7 can be correspondingly provided with three groups, and the three groups are respectively marked by red, yellow and green.
As shown in fig. 4, the liquid injection channel 41 is penetrated through the inner bottom of the liquid transfer tube 4, a top plate with a through hole is provided at the top of the liquid injection channel 41, a sealing rod 42 is movably sleeved in the liquid injection channel 41, the top end of the sealing rod 42 passes through the through hole upwards and extends to the outside of the top plate, a sealing block 43 capable of sealing the through hole is provided at the top end of the sealing rod 42, a pressure spring 44 is sleeved on the sealing rod 42 in the liquid injection channel 41, the bottom end of the pressure spring 44 is connected to the bottom end of the sealing rod 42, the top end of the pressure spring 44 is connected to the bottom surface of the top plate, and the sealing block 43 is sealed on the liquid injection channel 41 at the initial position of the pressure spring 44.
In cooperation, the liquid injection groove 21 is internally provided with a push rod 22 which can be inserted into the liquid injection channel 41, the push rod 22 is internally communicated with each flow passage, and the top side surface of the push rod 22 is provided with a liquid injection hole 23 communicated with the inside of the push rod 22. When the pipetting cylinder 4 is inserted into the liquid pouring groove 21, the ejector rod 22 enters the liquid pouring channel 41 and pushes the bottom end of the sealing rod 42, the pressure spring 44 is compressed under the limit of the top plate, and the pressure spring 44 drives the ejector rod 22 to move upwards, so that the sealing block 43 moves upwards to open the through hole; the liquid in the liquid transferring cylinder 4 is extruded by the piston push rod 5, the liquid in the liquid transferring cylinder 4 can enter the liquid injecting channel 41 from the through hole and then enter the liquid injecting hole 23 and enter the detecting grooves 25 from the flow passages, so that a certain amount of liquid can be injected into the detecting grooves 25 at the same time, and the working efficiency is improved. After the liquid injection is completed, the piston push rod 5 can be pulled outwards, so that the liquid in the flow channel can be extracted, or after the liquid-moving cylinder 4 is pulled out, the piston push rod 5 is pulled outwards for a certain distance, then the liquid-moving cylinder 4 is inserted into the liquid injection groove 21, and the piston push rod 5 is pushed again, so that the liquid in the flow channel can be blown into the detection groove 25.
The liquid injection amounts in the detection tanks 25 need not be identical, and only the bottom end of the sampling head 33 needs to be immersed in the liquid, that is, the liquid amount exceeds the detection tanks 25 by a certain height, so that the effect of protecting microorganisms can be achieved. Therefore, whether the volume of the liquid injected into each detection groove 25 in the flow channel is uniform is not a problem to be considered, if a part of the liquid in the detection grooves 25 is obviously less, the liquid can be injected independently, and if a part of the liquid in the detection grooves 25 is more, a part of the liquid can be extracted, so that overflow and diffusion of the excessive liquid are avoided. In the same manner, the cleaning of the inside of the ejector pin 22 and the flow path can be achieved by the above-described liquid injection method.
In one embodiment, a sealing ring 24 is provided on the ejector 22 adjacent to the injection hole 23 and mates with the injection channel 41, so that liquid overflows from the gap between the injection channel 41 and the ejector 22 when liquid enters the ejector 22 from the injection hole 23.
In one embodiment, the pipetting cylinder 4 with the piston push rod 5 can be placed in the operation handle 31, the rubber sleeve plug 32 is detachably arranged at the top of the operation handle 31, and the rubber sleeve plug 32 is tightly sleeved on the operation handle 31, so that the pipetting cylinder 4 or the piston push rod 5 is prevented from falling out.
When the microflora of the facial skin of the subject needs to be cultured, firstly, measuring the culture medium with the required volume by using a pipetting cylinder 4, and pouring the culture medium into a culture dish 1; according to the microorganism types and the abundance of the microorganisms on the face, a sampling head 33 with proper density gradient and length is selected to be mounted on a sampling disc, then the face of the subject is sampled, and each sampling area is recorded; the limiting pieces with the same color marked on the sampling plate are correspondingly placed in the culture dish 1 to culture microorganisms, so that further analysis of skin microorganisms in a region is facilitated.
For non-cultured microorganisms, selecting a proper detection box 2, placing the detection box 2 in a culture dish 1 according to marked limiting pieces with the same color, and injecting nucleic acid protection liquid or 16sRNA protection liquid into a detection groove 25 through a liquid injection groove 21 by using a liquid transfer cylinder 4; selecting a proper sampling head 33 to be mounted on a sampling disc, sampling the face of a subject, and recording each sampling area; the limiting pieces with the same color marked on the sampling plate are correspondingly placed in the culture dish 1, so that microorganisms at the bottom end of the sampling head 33 are immersed in the protective liquid, and the microorganisms are conveniently subjected to gene sequencing later.
Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims (9)

1. A skin microbial cultivation detection device, comprising:
The culture dish is characterized in that a plurality of limiting tables are uniformly arranged on the inner side surface of the culture dish at intervals, a detection box attached to the inner side of each limiting table is detachably placed in the culture dish, and a plurality of detection grooves are formed in the surface of each detection box in an array mode;
the sampling cover is provided with an operating handle on the top surface and a sampling disc on the bottom surface in a detachable way, sampling heads which can be placed in the detection groove are arranged on the bottom surface array of the sampling disc, the sampling disc can be covered in the culture dish, and the periphery of the bottom surface of the sampling disc can be placed on the limiting table;
A pipetting cylinder detachably placed in the handle for quantitatively injecting a desired liquid into the culture dish or the detection tank;
The middle part of the detection box is provided with a liquid injection groove matched with the liquid injection barrel, a plurality of flow passages which enable the liquid injection groove to be respectively communicated with the inner top sides of the detection grooves are arranged in the detection box, the inner bottom of the liquid injection barrel is provided with a liquid injection channel which can be communicated with the liquid injection groove, and a piston push rod which enables liquid in the liquid injection barrel to be injected into the liquid injection groove from the liquid injection channel is arranged in the liquid injection barrel in a matched mode.
2. A skin microbial cultivation testing device according to claim 1, wherein the sampling head is configured as a first sampling head immersed in the culture medium or as a second sampling head in contact with the surface of the culture medium.
3. A skin microorganism culture detection apparatus according to claim 1 or 2, wherein when the microorganism to be cultured comprises mold, the distance between adjacent sampling heads is 3 to 5cm.
4. The device according to claim 1, wherein a plurality of limiting members for positioning and placing are respectively arranged between the limiting table and the detection box and between the culture dish and the sampling plate correspondingly, and the plurality of limiting members divide the peripheral ring into at least three equal parts.
5. The device of claim 4, wherein the limiting member comprises a limiting groove and a limiting post matched with the limiting groove, the outer side surfaces of the detecting box and the sampling plate are provided with the limiting groove, and the inner side surfaces of the limiting table and the culture dish are provided with the limiting post.
6. The skin microorganism culture detection device according to claim 1, wherein the liquid injection channel is communicated with the inner bottom of the liquid transfer cylinder, a top plate with a through hole is arranged at the top of the liquid injection channel, a sealing rod is movably sleeved in the liquid injection channel, the top end of the sealing rod upwards passes through the through hole and extends to the outside of the top plate, a sealing block capable of sealing the through hole is arranged at the top end of the sealing rod, a pressure spring is sleeved on the sealing rod positioned in the liquid injection channel, the bottom end of the pressure spring is connected with the bottom end of the sealing rod, the top end of the pressure spring is connected with the bottom surface of the top plate, and the sealing block is sealed on the liquid injection channel due to the initial position of the pressure spring.
7. The device of claim 6, wherein a push rod is disposed in the liquid injection groove and can be inserted into the liquid injection channel, the push rod is communicated with each flow channel, and the top side of the push rod is provided with a liquid injection hole communicated with the inside.
8. The device of claim 7, wherein a sealing ring matched with the liquid injection channel is arranged on the pushing rod near the liquid injection hole.
9. The device according to claim 1, wherein the pipette cylinder with the piston push rod is disposed in the operating handle, and the rubber sleeve plug is detachably disposed on the top of the operating handle.
CN202322775443.5U 2023-10-16 2023-10-16 Skin microorganism cultivates detection device Active CN220887531U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202322775443.5U CN220887531U (en) 2023-10-16 2023-10-16 Skin microorganism cultivates detection device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202322775443.5U CN220887531U (en) 2023-10-16 2023-10-16 Skin microorganism cultivates detection device

Publications (1)

Publication Number Publication Date
CN220887531U true CN220887531U (en) 2024-05-03

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CN202322775443.5U Active CN220887531U (en) 2023-10-16 2023-10-16 Skin microorganism cultivates detection device

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