CN221165935U - Culture solution separate sampling device convenient for separate packaging and repeated use - Google Patents
Culture solution separate sampling device convenient for separate packaging and repeated use Download PDFInfo
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- CN221165935U CN221165935U CN202323267153.6U CN202323267153U CN221165935U CN 221165935 U CN221165935 U CN 221165935U CN 202323267153 U CN202323267153 U CN 202323267153U CN 221165935 U CN221165935 U CN 221165935U
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- split charging
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- container
- extraction
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- 238000004806 packaging method and process Methods 0.000 title claims abstract description 55
- 238000005070 sampling Methods 0.000 title abstract description 10
- 239000007788 liquid Substances 0.000 claims abstract description 35
- 238000000605 extraction Methods 0.000 claims abstract description 25
- 239000012528 membrane Substances 0.000 claims abstract description 6
- 239000000243 solution Substances 0.000 claims description 15
- 239000012531 culture fluid Substances 0.000 claims 7
- 239000011550 stock solution Substances 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 17
- 239000001963 growth medium Substances 0.000 description 8
- 238000000338 in vitro Methods 0.000 description 3
- 238000009630 liquid culture Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000007758 minimum essential medium Substances 0.000 description 2
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 1
- 239000012980 RPMI-1640 medium Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007877 drug screening Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000003104 tissue culture media Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a culture solution separate sampling device convenient for separate packaging and repeated use, and belongs to the technical field of experimental equipment. The liquid storage device comprises a liquid storage bottle, a split charging assembly and an extraction assembly, wherein the split charging assembly comprises a split charging container and a split charging pipe, the split charging pipe is connected with the split charging container, and an upper throttle valve and a lower throttle valve are respectively arranged at two ends of the split charging pipe; the liquid storage bottle is connected with the sub-packaging container through a liquid guide pipe, the sub-packaging container is connected with the extraction assembly through an exhaust pipe, and an air filter membrane is arranged on the exhaust pipe. The utility model provides a culture solution separate sampling device convenient for separate packaging and multiple use, wherein a culture solution original bottle is always in a sealed state in the use process so as to maintain a stable pH value, and meanwhile, the device has no excessive manual operation process in the operation process, and avoids factors possibly polluting the culture solution.
Description
Technical Field
The utility model relates to a culture solution separate sampling device convenient for separate packaging and repeated use, and belongs to the technical field of experimental equipment.
Background
In vitro culture is a biological research means for obtaining organism cells and tissues by anatomical separation and other methods and placing the organism cells and tissues in living environments similar to those in vivo so as to enable the organism cells and tissues to continue growing and developing. The nature of various life phenomena is clarified by researching the interrelation between cells and cells, between cells and tissues and between cells and microenvironments, and the model becomes a reliable model for in-vitro physiological and pathological researches and new drug screening.
In an in vitro culture process, a tissue culture medium is the basis for nutrients upon which cells and tissues survive. At present, conventional commercial liquid culture media such as DMEM (Dulbecco 'sModified Eagle's Medium), RPMI 1640, MEM (Minimum Essential Medium) and the like are very complete in formulation components and sterilization, and great convenience is provided for users. Large-package liquid cultures of 500ml and above purchased by users are generally recommended for sub-packaging. The method is mainly used for preventing the large-package culture solution from being polluted in multiple taking processes, so that the research progress is influenced, and serious economic loss is caused; in addition, most media have sodium bicarbonate (NaHCO 3) as the primary buffer system, wherein the pH and CO 2、HCO3 - are adjusted to maintain the pH of the liquid medium between 7.0 and 7.4, and deviations from this range may have deleterious effects on cell growth. In the process of taking the culture medium for many times, HCO 3 - in the solution overflows in the form of CO 2 due to the fact that the bottle cap is frequently opened, so that the pH of the culture medium is increased, and the color of the unused liquid culture medium is changed into powder during the effective period. Even so, during the dispensing process, contamination is easily caused due to some human handling factors.
Disclosure of utility model
In view of this, the utility model provides a culture solution separate sampling device which is convenient for separate packaging and multiple use, and the original culture solution bottle is always in a sealed state in the use process so as to maintain a stable pH value, and meanwhile, the utility model has no excessive manual operation process in the operation process, and avoids the factors which possibly pollute the culture solution.
The utility model provides a culture solution separate sampling device convenient for separate packaging and multiple use, which comprises a liquid storage bottle, a separate packaging assembly and a drawing assembly, wherein the separate packaging assembly comprises a separate packaging container and a separate packaging tube, the separate packaging tube is connected with the separate packaging container, and an upper throttle valve and a lower throttle valve are respectively arranged at two ends of the separate packaging tube;
The liquid storage bottle is connected with the sub-packaging container through a liquid guide pipe, the sub-packaging container is connected with the extraction assembly through an exhaust pipe, and an air filter membrane is arranged on the exhaust pipe.
Preferably, the bottom of the liquid guide tube penetrates through the liquid storage bottle cap at the top of the liquid storage bottle and is sealed with the liquid storage bottle cap, and the top of the liquid guide tube is positioned at one side of the split charging container.
Preferably, the top of the sub-packaging container is connected with the extraction assembly through an extraction pipe.
Preferably, the split charging container and the split charging pipe are respectively provided with a scale, and the scale on the split charging pipe is positioned between the upper throttle valve and the lower throttle valve.
Preferably, the sub-packaging container is a sub-packaging bag, and a hanging hole is formed in the sub-packaging bag.
Preferably, the sub-packaging container is a sub-packaging bottle, and the sub-packaging bottle is trapezoid with a large top and a small bottom.
Preferably, the extraction assembly is a manual gas cylinder.
Preferably, the extraction assembly is a vacuum pump.
The utility model has the beneficial effects that:
The utility model provides a culture solution separate sampling device convenient for separate packaging and multiple use, wherein a culture solution original bottle is always in a sealed state in the use process so as to maintain a stable pH value, and meanwhile, the device has no excessive manual operation process in the operation process, and avoids factors possibly polluting the culture solution.
Drawings
FIG. 1 is a schematic diagram of a culture solution separate sampling device which is convenient for separate packaging and multiple use.
FIG. 2 is a schematic view showing another construction of a separate culture medium collecting apparatus according to the present utility model which is convenient for sub-packaging and multi-use.
(1, Liquid storage bottle, 2, split charging container, 3, split charging pipe, 4, upper throttle valve, 5, lower throttle valve, 6, catheter, 7, exhaust pipe, 8, air filter membrane, 9, extraction component)
Detailed Description
Preferred embodiments of the present utility model will be described in detail below with reference to the accompanying drawings.
Embodiment one:
The utility model provides a culture solution separate sampling device convenient for separate packaging and multiple use, which comprises a liquid storage bottle 1, a separate packaging assembly and a drawing assembly 9, wherein the separate packaging assembly comprises a separate packaging container 2 and a separate packaging tube 3, the separate packaging tube 3 is connected with the separate packaging container 2, and an upper throttle valve 4 and a lower throttle valve 5 are respectively arranged at two ends of the separate packaging tube 3;
The liquid storage bottle 1 is connected with the sub-packaging container 2 through being provided with a liquid guide tube 6, the sub-packaging container 2 is connected with the extraction assembly 9 through being provided with an extraction tube 7, and an air filter membrane 8 is arranged on the extraction tube 7.
The bottom of the liquid guide tube 6 penetrates through the liquid storage bottle cap at the top of the liquid storage bottle 1 and is sealed with the liquid storage bottle cap, and the top of the liquid guide tube 6 is positioned at one side of the split charging container 2.
The top of the sub-packaging container 2 is connected with the extraction assembly 9 through an extraction pipe 7.
The split charging container 2 and the split charging pipe 3 are respectively provided with a scale, and the scale on the split charging pipe 3 is positioned between the upper throttle valve 4 and the lower throttle valve 5.
The sub-packaging container 2 is a sub-packaging bag, and a hanging hole is arranged on the sub-packaging bag.
The extraction assembly 9 is a manual extraction cylinder.
In the use process of the embodiment, the split charging container is hung at a higher position through the hanging hole, the bottom of the liquid guide tube 6 is inserted into the liquid storage bottle 1, then the air in the split charging container 2 is extracted through manually pressing the extracting component 9, the air filter membrane 8 is an aseptic section to the extracting tube 7 of one section of the split charging container 2, the culture medium in the liquid storage bottle 1 flows into the split charging container 2 through the liquid guide tube 6 by pressing the extracting component 9, the quantity of the culture medium can be controlled according to the scale on the split charging tube 3 by opening the upper throttle valve 4 when the split charging container is needed, and the split charging work is completed by closing the upper throttle valve and opening the lower throttle valve 5 after the split charging container flows to a specified value.
Embodiment two:
The sub-packaging container 2 is a sub-packaging bottle which is trapezoid and has a big top and a small bottom; the extraction assembly 9 is a vacuum pump
In the using process of the embodiment, the fixing sleeve is sleeved on the split charging bottle to fix, and when the split charging bottle is used, the vacuum pump 9 is opened to pump air in the split charging container 2, so that the culture medium in the liquid storage bottle 1 flows into the split charging container 2 through the liquid guide tube 6.
The utility model and its embodiments have been described above with no limitation, and the actual construction is not limited to the embodiments of the utility model as shown in the drawings. In summary, if one of ordinary skill in the art is informed by this disclosure, a structural manner and an embodiment similar to the technical solution should not be creatively devised without departing from the gist of the present utility model.
Claims (8)
1. The utility model provides a device is adopted to culture solution branch convenient to partial shipment and repetitious usage, includes stock solution bottle (1), partial shipment subassembly, extraction subassembly (9), its characterized in that: the split charging assembly comprises a split charging container (2) and a split charging tube (3), wherein the split charging tube (3) is connected with the split charging container (2), and an upper throttle valve (4) and a lower throttle valve (5) are respectively arranged at two ends of the split charging tube (3);
The liquid storage bottle (1) is connected with the sub-packaging container (2) through being provided with a liquid guide tube (6), the sub-packaging container (2) is connected with the extraction assembly (9) through being provided with an extraction tube (7), and an air filter membrane (8) is arranged on the extraction tube (7).
2. The device for separate collection of culture fluid for convenient sub-packaging and repeated use according to claim 1, wherein: the bottom of the liquid guide tube (6) penetrates through the liquid storage bottle cap at the top of the liquid storage bottle (1) and is sealed with the liquid storage bottle cap, and the top of the liquid guide tube (6) is positioned at one side of the split charging container (2).
3. The device for separate collection of culture fluid for convenient sub-packaging and repeated use according to claim 1, wherein: the top of the sub-packaging container (2) is connected with the extraction assembly (9) through an extraction pipe (7).
4. The device for separate collection of culture fluid for convenient sub-packaging and repeated use according to claim 1, wherein: the split charging container (2) and the split charging tube (3) are respectively provided with a scale, and the scale on the split charging tube (3) is positioned between the upper throttle valve (4) and the lower throttle valve (5).
5. The device for separate collection of culture fluid for convenient sub-packaging and repeated use according to claim 1, wherein: the split charging container (2) is a split charging bag, and a hanging hole is formed in the split charging bag.
6. The device for separate collection of culture fluid for convenient sub-packaging and repeated use according to claim 1, wherein: the split charging container (2) is a split charging bottle, and the split charging bottle is trapezoid with big top and small bottom.
7. The device for separate collection of culture fluid for convenient sub-packaging and repeated use according to claim 1, wherein: the extraction component (9) is a manual air extraction bottle.
8. The device for separate collection of culture fluid for convenient sub-packaging and repeated use according to claim 1, wherein: the extraction assembly (9) is a vacuum pump.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202323267153.6U CN221165935U (en) | 2023-12-01 | 2023-12-01 | Culture solution separate sampling device convenient for separate packaging and repeated use |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202323267153.6U CN221165935U (en) | 2023-12-01 | 2023-12-01 | Culture solution separate sampling device convenient for separate packaging and repeated use |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN221165935U true CN221165935U (en) | 2024-06-18 |
Family
ID=91441705
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202323267153.6U Active CN221165935U (en) | 2023-12-01 | 2023-12-01 | Culture solution separate sampling device convenient for separate packaging and repeated use |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN221165935U (en) |
-
2023
- 2023-12-01 CN CN202323267153.6U patent/CN221165935U/en active Active
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