CN115029285B - Method for harmlessly and biologically degrading aureomycin residues by utilizing composite microorganisms and hermetia illucens - Google Patents
Method for harmlessly and biologically degrading aureomycin residues by utilizing composite microorganisms and hermetia illucens Download PDFInfo
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- CYDMQBQPVICBEU-XRNKAMNCSA-N chlortetracycline Chemical group C1=CC(Cl)=C2[C@](O)(C)[C@H]3C[C@H]4[C@H](N(C)C)C(O)=C(C(N)=O)C(=O)[C@@]4(O)C(O)=C3C(=O)C2=C1O CYDMQBQPVICBEU-XRNKAMNCSA-N 0.000 title claims abstract description 59
- 244000005700 microbiome Species 0.000 title claims abstract description 42
- 241000709785 Hermetia illucens Species 0.000 title claims abstract description 32
- 239000002131 composite material Substances 0.000 title claims abstract description 31
- 238000000034 method Methods 0.000 title claims abstract description 27
- 230000000593 degrading effect Effects 0.000 title claims abstract description 18
- 150000001875 compounds Chemical class 0.000 claims abstract description 45
- 230000000813 microbial effect Effects 0.000 claims abstract description 28
- CYDMQBQPVICBEU-UHFFFAOYSA-N chlorotetracycline Natural products C1=CC(Cl)=C2C(O)(C)C3CC4C(N(C)C)C(O)=C(C(N)=O)C(=O)C4(O)C(O)=C3C(=O)C2=C1O CYDMQBQPVICBEU-UHFFFAOYSA-N 0.000 claims abstract description 27
- 229960004475 chlortetracycline Drugs 0.000 claims abstract description 27
- 235000019365 chlortetracycline Nutrition 0.000 claims abstract description 27
- 230000012447 hatching Effects 0.000 claims abstract description 22
- 239000000463 material Substances 0.000 claims abstract description 20
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 10
- 241000233866 Fungi Species 0.000 claims abstract description 5
- 241000382353 Pupa Species 0.000 claims abstract description 5
- 239000003795 chemical substances by application Substances 0.000 claims description 27
- 241000238631 Hexapoda Species 0.000 claims description 17
- 235000013601 eggs Nutrition 0.000 claims description 15
- 238000002360 preparation method Methods 0.000 claims description 14
- 239000001963 growth medium Substances 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 238000000855 fermentation Methods 0.000 claims description 8
- 230000004151 fermentation Effects 0.000 claims description 8
- 239000004615 ingredient Substances 0.000 claims description 8
- 238000009630 liquid culture Methods 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 5
- 230000001105 regulatory effect Effects 0.000 claims description 5
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 4
- 241000589155 Agrobacterium tumefaciens Species 0.000 claims description 4
- 241000193755 Bacillus cereus Species 0.000 claims description 4
- 244000063299 Bacillus subtilis Species 0.000 claims description 4
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 4
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 4
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 4
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 4
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 4
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 4
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 4
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 4
- 229940041514 candida albicans extract Drugs 0.000 claims description 4
- 235000020415 coconut juice Nutrition 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 4
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 4
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 4
- 108010009004 proteose-peptone Proteins 0.000 claims description 4
- 235000017281 sodium acetate Nutrition 0.000 claims description 4
- 239000001632 sodium acetate Substances 0.000 claims description 4
- 239000012138 yeast extract Substances 0.000 claims description 4
- 238000011534 incubation Methods 0.000 claims description 3
- 230000002195 synergetic effect Effects 0.000 claims 2
- 230000015556 catabolic process Effects 0.000 abstract description 10
- 238000006731 degradation reaction Methods 0.000 abstract description 10
- 230000008569 process Effects 0.000 abstract description 5
- 235000015097 nutrients Nutrition 0.000 abstract description 3
- 230000003115 biocidal effect Effects 0.000 abstract description 2
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- 239000000203 mixture Substances 0.000 abstract 1
- 239000007789 gas Substances 0.000 description 8
- 239000002893 slag Substances 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 5
- 244000144972 livestock Species 0.000 description 5
- 244000144977 poultry Species 0.000 description 5
- 239000004099 Chlortetracycline Substances 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 210000003608 fece Anatomy 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- MUBZPKHOEPUJKR-UHFFFAOYSA-N oxalic acid group Chemical group C(C(=O)O)(=O)O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 244000046052 Phaseolus vulgaris Species 0.000 description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- 239000010871 livestock manure Substances 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000010815 organic waste Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical class N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- 241000186984 Kitasatospora aureofaciens Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- LSDPWZHWYPCBBB-UHFFFAOYSA-N Methanethiol Chemical compound SC LSDPWZHWYPCBBB-UHFFFAOYSA-N 0.000 description 1
- 239000010868 animal carcass Substances 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 239000010806 kitchen waste Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 235000006408 oxalic acid Nutrition 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
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- 230000001737 promoting effect Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- C12N1/14—Fungi; Culture media therefor
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Abstract
The invention relates to a method for harmlessly and biologically degrading aureomycin residues by utilizing composite microorganisms and hermetia illucens, belonging to the technical field of antibiotic degradation. Solves the problems that the nutrient components in the aureomycin residues are complex, aureomycin residues are contained, and malodorous gas is generated in the treatment process of the aureomycin residues in the prior art. The method for biodegrading aureomycin fungi residues comprises the steps of preparing compound microorganisms, preparing a compound microbial agent, compounding the compound microbial agent with aureomycin fungi residues and wheat bran, putting the black soldier fly larvae of 3 days old after hatching into a compound material, and treating the mixture in an environment with the relative humidity of 40RH at the temperature of 25-28 ℃ until the black soldier fly pre-pupa worms appear. According to the method, the compound microbial agent is used for converting and degrading the aureomycin residues in cooperation with the hermetia illucens larvae, so that the degradation efficiency is high, and the odor generated in the treatment process is effectively removed, so that the innocent treatment and the resource utilization of the aureomycin residues are realized.
Description
Technical Field
The invention belongs to the technical field of antibiotic degradation, and particularly relates to a method for harmlessly and biologically degrading aureomycin residues by utilizing composite microorganisms and hermetia illucens.
Background
Chlortetracycline (chlortetracycline), chemical formula C 22 H 23 ClN 2 O 8 Is golden yellow crystal powder, and is produced by fermentation of streptomyces aureofaciens.
The aureomycin has remarkable effect on promoting the growth of livestock and poultry and preventing epidemic diseases, and becomes the first choice of antibiotics in livestock and poultry raising industry. During the production process of aureomycin, a large amount of aureomycin residues can be produced. The fungus dreg contains rich nutrients, such as coarse protein (20-50%) and Ca, P, S and other trace elements, and also includes great amount of mycelium, fermentation metabolite, culture medium degradation product, aureomycin residue and other medicine components. As the organic matter content is high, the bacterial residues are commonly used as livestock and poultry feed additives, but the residual aureomycin is enriched in animal bodies, only a small part of the residual aureomycin is absorbed and utilized, 30% -90% of aureomycin is discharged from the bodies in the form of original or metabolic products, and the residual aureomycin in livestock and poultry manure is high. The feces are directly applied to the field without decomposition, so that the balance of soil microorganisms is destroyed, the generation of bacterial drug resistance is promoted, and after the feces are absorbed and accumulated by crops, the food chlortetracycline is polluted, and finally the health of human beings is endangered. To reduce this hazard, the aureomycin residues need to be treated. However, the volatile hydrogen sulfide, mercaptan, ammonia compounds and other malodorous gases generated in the treatment process of the chlortetracycline fungi residues cannot be effectively treated, so that secondary environmental pollution is caused. Therefore, a method capable of harmlessly biodegrading aureomycin residues is needed.
The black soldier fly larvae are wide in feeding habit, and can eat livestock and poultry manure, kitchen waste, food processing byproducts, animal carcasses, waste residues and the like. The intestinal flora, the metabolic system and the immune system in the body play a vital role in the treatment of organic wastes. The biological conversion method can convert the nutrition components in the organic waste into insect self-nutrition and metabolite insect sand, has the advantages of environmental protection, safety, biological conversion, high resource utilization rate and the like, and is widely paid attention to both home and abroad. For example, chinese patent 2020100791997 discloses a compound hermetia illucens microbial preparation, a preparation method thereof and application thereof in conversion of bean dregs, and aims at the goal of the soybean dregs, and aims at realizing efficient conversion and utilization of the bean dregs by directionally changing intestinal microbial components of the hermetia illucens.
Disclosure of Invention
The invention aims to solve the problems that the nutrient components in aureomycin residues are complex, aureomycin residues are contained and malodorous gas is generated in the treatment process of the aureomycin residues in the prior art, and provides a method for harmlessly and biologically degrading aureomycin residues by utilizing compound microorganisms and hermetia illucens.
In order to achieve the above purpose, the following technical scheme is adopted:
the invention provides a method for harmlessly and biologically degrading aureomycin residues by utilizing composite microorganisms and hermetia illucens, which comprises the following steps:
step one, preparation of composite microorganism
According to weight portions, 17-22 portions of bacillus subtilis, 8-10 portions of bacillus cereus, 18-20 portions of beer yeast, 16-20 portions of lactobacillus acidophilus, 8-10 portions of rhizobium radiobacter and 16-18 portions of lactobacillus plantarum are uniformly mixed to obtain compound microorganisms;
step two, preparation of composite microbial agent
Inoculating the composite microorganism into a liquid culture medium, and fermenting to obtain a composite microorganism microbial agent;
step three, preparation of compound ingredients
Uniformly mixing aureomycin residues and wheat bran according to the mass ratio of 1 (10-20) to obtain a base material, adding a composite microbial agent into the base material according to the mass ratio of the composite microbial agent to the base material of 1:500, and regulating the water content to 60-70% to obtain a compound material;
step four, degrading aureomycin residues
The black soldier fly larvae of 3 days after hatching are put into compound ingredients and are placed in an environment with the temperature of 25 ℃ to 28 ℃ and the relative humidity of 40RH to be treated until the pre-pupa insects appear;
the proportion of the throwing is as follows: 0.50g of hermetia illucens larva hatched with the worm eggs is put into each 2.5kg of the compound.
Preferably, in the second step, the fermentation temperature is 30-36 ℃ and the fermentation time is 15-20 days.
Preferably, in the second step, the liquid culture medium comprises 3-6wt% of brown sugar, 7-9wt% of maltose, 0.5-1.5wt% of yeast extract, 0.5-1.0wt% of casein peptone, 0.4-0.6wt% of sodium acetate and MgSO 4 ·7H 2 O0.2-0.5wt％，FeCl 3 0.01-0.02wt％，CaCO 3 1-2.5wt% and the balance of sterilized coconut water.
Preferably, in the fourth step, the black soldier fly larvae are obtained by the following method:
placing 0.50g of insect eggs on a 40-mesh screen, placing a hatching box below the screen, taking wheat bran with the water content of 65-70% as an open feed for the initially hatched larvae in the hatching box, and placing the insect eggs into the hatching box after hatching, and culturing until the insect eggs are 3 days old.
Further, the incubation and culture temperatures are 28-32 ℃ and the relative humidity is 70-75RH.
Compared with the prior art, the invention has the beneficial effects that:
according to the method for harmlessly and biologically degrading the aureomycin residues by the aid of the compound microorganisms and the hermetia illucens, the compound microorganism preparation is added into the aureomycin residues according to a certain proportion, the aureomycin residues are biologically degraded by the aid of the compound microorganism and the hermetia illucens, degradation efficiency is high, odor is not generated, and accordingly harmless treatment and recycling of the aureomycin residues are achieved, and experiments prove that:
1. with the digestion of the black soldier fly larvae on the aureomycin residues, the degradation rate of aureomycin in the residues is gradually increased, and the degradation rate of aureomycin added with the compound microbial agent group is 71.08% (P is less than 0.05) which is higher than 63.76% (P is less than 0.05) of the degradation rate of aureomycin not added with the compound microbial agent group;
2. as can be seen from Table 2, the addition of the complex microorganism group was effective for NH in malodor, as compared with the case where the complex microorganism group was not added 3 And H 2 The removal rate of the S gas reaches more than 65 percent.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a graph showing the degradation rate of aureomycin in the slag with and without the addition of the complex microbial agent of example 1 of the present invention.
Detailed Description
For a further understanding of the present invention, preferred embodiments of the invention are described below, but it is to be understood that these descriptions are merely intended to illustrate further features and advantages of the invention, and are not limiting of the claims of the invention.
The method for harmlessly and biologically degrading aureomycin residues by utilizing the compound microorganism and the hermetia illucens comprises the following steps of:
step one, preparation of composite microorganism
According to weight portions, 17-22 portions of bacillus subtilis, 8-10 portions of bacillus cereus, 18-20 portions of beer yeast, 16-20 portions of lactobacillus acidophilus, 8-10 portions of rhizobium radiobacter and 16-18 portions of lactobacillus plantarum are uniformly mixed to obtain compound microorganisms;
step two, preparation of composite microbial agent
Inoculating the composite microorganism into a liquid culture medium, and fermenting to obtain a composite microorganism microbial agent;
step three, preparation of compound ingredients
Uniformly mixing aureomycin residues and wheat bran according to the mass ratio of 1 (10-20) to obtain a base material, adding a composite microbial agent into the base material according to the mass ratio of the composite microbial agent to the base material of 1:500, and regulating the water content to 60-70% to obtain a compound material;
step four, degrading aureomycin residues
The black soldier fly larvae of 3 days after hatching are put into compound ingredients and are placed in an environment with the temperature of 25 ℃ to 28 ℃ and the relative humidity of 40RH to be treated until the pre-pupa insects appear;
wherein, the proportion of the addition is that 0.5g of hermetia illucens larva hatched by the worm eggs is added into each 2.5kg of compound.
In the above technical scheme, in the second step, the fermentation temperature is preferably 30-36 ℃, and the fermentation time is preferably 15-20 days.
In the technical scheme, in the second step, the liquid culture medium comprises 3-6wt% of brown sugar, 7-9wt% of maltose, 0.5-1.5wt% of yeast extract, 0.5-1.0wt% of casein peptone, 0.4-0.6wt% of sodium acetate and MgSO (MgSO) 4 ·7H 2 O0.2-0.5wt％，FeCl 3 0.01-0.02wt％，CaCO 3 1-2.5wt% and the balance of sterilized coconut water.
In the technical scheme, in the fourth step, the black soldier fly larvae are obtained by the following method: placing 0.50g of insect eggs on a 40-mesh screen, placing a hatching box below the screen, taking wheat bran with the water content of 65-70% as an open feed for the initially hatched larvae in the hatching box, and placing the insect eggs into the hatching box after hatching, and culturing until the insect eggs are 3 days old. Preferably, the incubation and culture temperatures are 28-32deg.C and the relative humidity is 70-75RH.
The terms used in the present invention generally have meanings commonly understood by those of ordinary skill in the art unless otherwise indicated. In order to enable those skilled in the art to better understand the technical solutions of the present invention, the present invention will be described in further detail with reference to examples.
In the following examples, various processes and methods, which are not described in detail, are conventional methods well known in the art. Materials, reagents, devices, instruments, equipment and the like used in the examples described below are commercially available unless otherwise specified.
The invention is further illustrated below with reference to examples.
Example 1
Step one, preparation of composite microorganism
According to weight portions, 17-22 portions of bacillus subtilis, 8-10 portions of bacillus cereus, 18-20 portions of beer yeast, 16-20 portions of lactobacillus acidophilus, 8-10 portions of rhizobium radiobacter and 16-18 portions of lactobacillus plantarum are uniformly mixed to obtain compound microorganisms;
step two, preparation of composite microbial agent
Inoculating the compound microorganism into a liquid culture medium, and fermenting for 15-20 days at 30-36 ℃ to obtain a compound microorganism microbial inoculum;
wherein the liquid culture medium comprises brown sugar 3-6wt%, maltose 7-9wt%, yeast extract 0.5-1.5wt%, casein peptone 0.5-1.0wt%, sodium acetate 0.4-0.6wt%, and MgSO 4 ·7H 2 O0.2-0.5wt％，FeCl 3 0.01-0.02wt％，CaCO 3 1-2.5wt% and the balance of sterilized coconut water;
step three, compounding ingredients
Experiment group (adding compound microorganism bacterium agent group)
Uniformly mixing aureomycin residues and wheat bran according to the mass ratio of 1 (10-20) to obtain a base material, adding a composite microbial agent into the base material according to the mass ratio of the composite microbial agent to the base material of 1:500, and regulating the water content to 60-70% to obtain a compound material;
control group (without adding compound microorganism bacterium agent group)
Uniformly mixing aureomycin residues and wheat bran according to the mass ratio of 1 (10-20) to obtain a base material, and regulating the water content to 60% -70% to obtain a compound material;
step four, degrading aureomycin residues
Placing 0.50g of insect eggs on a 40-mesh screen, arranging a hatching box below the screen, taking wheat bran with the water content of 65-70% as an open feed for the primary hatching larvae in the hatching box, placing the insect eggs into the hatching box after hatching, and culturing until the insect eggs are 3 days old, wherein the temperature of a hatching chamber is 28-32 ℃, and the relative humidity is 70-75RH;
putting the hatched 3-day-old larvae into 2.5kg of the two groups of compound ingredients in the third step, and treating the larvae in an environment with the relative humidity of 40RH at the temperature of 25-28 ℃;
every 2 days, namely, days 0, 2, 4, 6, 8 and 10, randomly sampling and collecting 100 g+/-10 g of slag samples until the pre-pupa appears in the culture for 10 days, collecting all groups of slag samples, and drying at 60 ℃ for 8 hours to constant weight; measuring the content of aureomycin in the slag by adopting high performance liquid chromatography; the method for measuring the aureomycin content in the slag by high performance liquid chromatography comprises the following steps: the chromatographic conditions are adopted, and the mobile phase is oxalic acid solution [ c (1/2H) 2 C 2 O 4 )＝0.01mol/L]Acetonitrile: methanol=75:15:10; the flow rate is 1mL/min, the detector is an ultraviolet detector, the detection wavelength is 370nm, and the sample injection amount is 10 mu L); the chromatographic column is Agilent C18 chromatographic column (column length 150mm, inner diameter 4.6mm, particle size 5 μm), and the test results are shown in Table 1 and FIG. 1;
TABLE 1
As can be seen from table 1 and fig. 1, as the black soldier fly larvae digest aureomycin residues, the degradation rate of aureomycin in the residues gradually increases, wherein the degradation rate of aureomycin in the group added with the compound microbial agent is 71.08% (P < 0.05) which is higher than 63.76% (P < 0.05) of the group not added with the compound microbial agent;
meanwhile, every 2 days, namely days 0, 2, 4, 6, 8 and 10, respectively collecting the compound microbial agent group added and the slag without adding the compound microbial agent group by using a sealed container to release gas, and measuring NH in the gas 3 Concentration and H 2 S gas concentration, comparing the odor removal rate of the compound microorganism bacterium agent group with the odor removal rate of the compound microorganism bacterium agent group without the compound microorganism bacterium agent group, and testing results are shown in table 2;
as can be seen from Table 2, the addition of the complex microorganism group was effective for NH in malodor, as compared with the case where the complex microorganism group was not added 3 And H 2 The removal rate of the S gas reaches more than 65 percent.
TABLE 2 composite microbial inoculant NH in slag 3 And H 2 S gas removal rate
It is apparent that the above embodiments are merely examples for clarity of illustration and are not limiting examples. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. And obvious variations or modifications thereof are contemplated as falling within the scope of the present invention.
Claims (5)
1. The method for harmlessly and biologically degrading aureomycin residues by utilizing the composite microorganism and the hermetia illucens is characterized by comprising the following steps of:
step one, preparation of composite microorganism
According to weight portions, 17-22 portions of bacillus subtilis, 8-10 portions of bacillus cereus, 18-20 portions of beer yeast, 16-20 portions of lactobacillus acidophilus, 8-10 portions of rhizobium radiobacter and 16-18 portions of lactobacillus plantarum are uniformly mixed to obtain compound microorganisms;
step two, preparation of composite microbial agent
Inoculating the composite microorganism into a liquid culture medium, and fermenting to obtain a composite microorganism microbial agent;
step three, preparation of compound ingredients
Uniformly mixing aureomycin residues and wheat bran according to the mass ratio of 1 (10-20) to obtain a base material, adding a composite microbial agent into the base material according to the mass ratio of the composite microbial agent to the base material of 1:500, and regulating the water content to 60-70% to obtain a compound material;
step four, degrading aureomycin residues
The black soldier fly larvae of 3 days after hatching are put into compound ingredients and are placed in an environment with the temperature of 25 ℃ to 28 ℃ and the relative humidity of 40RH to be treated until the pre-pupa insects appear;
the proportion of the throwing is as follows: 0.50g of hermetia illucens larva hatched with the worm eggs is put into each 2.5kg of the compound.
2. The method for harmlessly and biologically degrading aureomycin by utilizing the composite microorganism and the hermetia illucens as claimed in claim 1, wherein in the second step, the fermentation temperature is 30-36 ℃ and the fermentation time is 15-20 days.
3. The method for harmlessly and biologically degrading aureomycin by utilizing the synergistic action of the compound microorganisms and the hermetia illucens as claimed in claim 1, wherein in the second step, the liquid culture medium comprises 3-6wt% of brown sugar, 7-9wt% of maltose, 0.5-1.5wt% of yeast extract, 0.5-1.0wt% of casein peptone, 0.4-0.6wt% of sodium acetate and MgSO (MgSO) 4 ·7H 2 O0.2-0.5wt％，FeCl 3 0.01-0.02wt％，CaCO 3 1-2.5wt% and the balance of sterilized coconut water.
4. The method for harmlessly and biologically degrading aureomycin fungi residues by utilizing the composite microorganism and the hermetia illucens as well as the hermetia illucens as disclosed in claim 1, wherein in the fourth step, the hermetia illucens larvae are obtained by the following method:
placing 0.50g of insect eggs on a 40-mesh screen, placing a hatching box below the screen, taking wheat bran with the water content of 65-70% as an open feed for the initially hatched larvae in the hatching box, and placing the insect eggs into the hatching box after hatching, and culturing until the insect eggs are 3 days old.
5. The method for harmlessly and biologically degrading aureomycin by utilizing the synergistic action of the compound microorganisms and the hermetia illucens as claimed in claim 4, wherein the incubation and the culture are carried out at the temperature of 28-32 ℃ and the relative humidity of 70-75RH.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106614398A (en) * | 2016-12-19 | 2017-05-10 | 江苏大学 | Method for performing pig manure resource treatment by utilizing hermetia illucens |
| CN109122593A (en) * | 2018-08-31 | 2019-01-04 | 武汉工程大学 | Utilize the method for antibiotic pollution in black soldier flies larva degradation repairing environment |
| MX2017014395A (en) * | 2017-11-09 | 2019-05-10 | Javier Villarreal Lozano Gerardo | Method and process for the treatment and biological degradation of difficult to degrade organic solid residues. |
| CN111172075A (en) * | 2020-02-03 | 2020-05-19 | 南京工业大学 | Hermetia illucens compound microbial preparation, preparation method thereof and application thereof in bean dreg conversion |
| CN113373078A (en) * | 2021-02-09 | 2021-09-10 | 南京工业大学 | Compound microorganism and application thereof in combination with black soldier fly to transform edible fungus residues |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110896925B (en) * | 2019-10-31 | 2021-07-13 | 新疆农业大学 | Method for continuously converting kitchen waste by utilizing three environmental insects |
-
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106614398A (en) * | 2016-12-19 | 2017-05-10 | 江苏大学 | Method for performing pig manure resource treatment by utilizing hermetia illucens |
| MX2017014395A (en) * | 2017-11-09 | 2019-05-10 | Javier Villarreal Lozano Gerardo | Method and process for the treatment and biological degradation of difficult to degrade organic solid residues. |
| CN109122593A (en) * | 2018-08-31 | 2019-01-04 | 武汉工程大学 | Utilize the method for antibiotic pollution in black soldier flies larva degradation repairing environment |
| CN111172075A (en) * | 2020-02-03 | 2020-05-19 | 南京工业大学 | Hermetia illucens compound microbial preparation, preparation method thereof and application thereof in bean dreg conversion |
| CN113373078A (en) * | 2021-02-09 | 2021-09-10 | 南京工业大学 | Compound microorganism and application thereof in combination with black soldier fly to transform edible fungus residues |
Non-Patent Citations (2)
| Title |
|---|
| Systematic characterization and proposed pathway of tetracycline degradation in solid waste treatment by Hermetia illucens with intestinal microbiota;Minmin Cai et al.;《Environmental Pollution》;第242卷;第634-642页 * |
| 黑水虻对金霉素菌渣的降解调控分析;王会;《昆虫学报》;第66卷(第10期);第135-1361页 * |
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