CN114982972A - Method for preparing qi and yin deficiency health-care capsule food by selecting blood meat poultry animal to extract high-quality protein and Chinese herbal medicines - Google Patents
Method for preparing qi and yin deficiency health-care capsule food by selecting blood meat poultry animal to extract high-quality protein and Chinese herbal medicines Download PDFInfo
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- CN114982972A CN114982972A CN202210686541.9A CN202210686541A CN114982972A CN 114982972 A CN114982972 A CN 114982972A CN 202210686541 A CN202210686541 A CN 202210686541A CN 114982972 A CN114982972 A CN 114982972A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The invention discloses a method for preparing qi-yin-deficiency health-care capsule food by selecting blood meat poultry animals to extract high-quality protein and Chinese herbal medicines. The method comprises the following steps: (1) sequentially adding minced blood meat poultry and water into an extraction container, heating for extraction, opening a condensation valve when the temperature of an extraction liquid reaches 105 ℃, till the surface liquid is distilled, and filtering to obtain a filtrate for later use; (2) adding adjuvants into the filtrate, and stirring to obtain high quality protein nutrient solution extracted from blood meat poultry animal; (3) and (3) adding the Chinese herbal medicine composite functional powder into the nutrient solution obtained in the step (2), uniformly stirring, performing spray drying, collecting, filling capsules, and packaging to obtain the qi and yin deficiency health-care capsule food. The capsule food produced by the preparation method provided by the invention can better extract and retain nutrient components, has the functions of tonifying qi and yin, nourishing liver and kidney, promoting blood circulation and soothing nerves and has the health-care effect of enhancing the immunity of a human body aiming at sub-health caused by deficiency of qi and yin and the physique of people needing to be well-maintained.
Description
Technical Field
The invention belongs to the technical field of functional foods, and particularly relates to a method for preparing qi-yin-deficiency health-care capsule food by selecting blood meat poultry animals to extract high-quality protein and Chinese herbal medicines.
Background
According to the '2020 health medical prediction report' issued by the morals, the global population is rapidly moving to the aging stage, the population of China also enters the severe aging stage, and according to incomplete statistics, the number of old people over 60 years old in China reaches over 2 hundred million at the present stage. With the increasing aging progress of the population, a series of social problems are generated. Especially, the old people have gradually declined physiological functions, so that the immune function is reduced, and the risk of diseases is increased. From the perspective of preventive treatment and health care, prevention, treatment and improvement of the immune function of the elderly become one of the main directions for preventing chronic diseases of the elderly.
According to related researches, the elderly people above 60 have constitution of qi and yin deficiency, and long-term qi and yin deficiency can cause the immunity of the human body to be reduced, particularly the elderly population, and the prevalence rate of the people above 60 years old is up to 56% in recent years. Aiming at the current situation of qi and yin deficiency, the health food with dietary therapy effect is developed, and the urgent requirements of the middle-aged and old-aged consumer groups suffering from qi and yin deficiency can be met.
Disclosure of Invention
The invention aims to provide a method for preparing qi-yin-deficiency health-preserving capsule food by using high-quality protein extracted from blood-flesh poultry and Chinese herbal medicines aiming at sub-health of qi-yin deficiency and physique of people needing health preservation. The capsule food produced by the preparation method provided by the invention can better extract and retain nutrient components, has the functions of tonifying qi and yin, nourishing liver and kidney, promoting blood circulation and soothing nerves and has the health-care effect of enhancing the immunity of a human body aiming at sub-health caused by qi and yin deficiency and the physique of people needing to be well-maintained.
In order to achieve the purpose, the invention adopts the technical scheme that: a method for preparing qi and yin deficiency health-care capsule food by selecting and extracting high-quality protein from blood meat poultry and Chinese herbal medicines comprises the following steps:
(1) sequentially adding the minced blood-meat poultry and water into an extraction container, heating and extracting, opening a condensation valve when the temperature of the extract liquid reaches 105 ℃ until the surface liquid is distilled, and filtering to obtain filtrate for later use;
(2) adding adjuvants into the filtrate, and stirring to obtain high quality protein nutrient solution extracted from blood meat poultry animal;
(3) and (3) adding the Chinese herbal medicine composite functional powder into the nutrient solution obtained in the step (2), uniformly stirring, performing spray drying, collecting, filling capsules, and packaging to obtain the qi and yin deficiency health-care capsule food.
According to the application research of the traditional Chinese medicine theory and the modern medicine to the biotechnology field, the invention proves that the immunity of the human body can be obviously enhanced by supplementing a certain content of high-quality animal protein and natural edible plant medicines in the diet. The poultry provided by the invention is selected from a biological laboratory or a special production and operation place which is qualified in quarantine and meets the requirements of food safety standards.
Preferably, the blood-fleshed poultry pieces in step (1) are obtained by the following steps: slaughtering poultry to remove hair, removing head and viscera to obtain blood meat poultry, cleaning, and chopping to obtain blood meat poultry pieces.
Preferably, the poultry in step (1) includes silky fowl, rabbit, tortoise, crocodile and pigeon.
The invention discloses a black-bone chicken: enter liver and kidney meridians. Sweet in nature and mild in taste. Tonify liver and kidney, replenish qi and blood, and reduce deficiency heat. A rabbit: entering the liver; the large intestine channel. Sweet in taste; cold in nature; invigorating spleen and strengthening middle warmer; cool blood and remove toxicity. Turtle: entering liver, lung and spleen; sweet and sour with warm nature; removing damp arthralgia, nourishing yin, replenishing kidney water, stopping bleeding, and removing toxic substance. The crocodile has the functions of tonifying qi and blood, nourishing heart and lung, strengthening bones and muscles and expelling damp evil. Pigeon: entering the lung; liver; the kidney channel. Salty taste; the nature is mild. Nourishing kidney, invigorating qi, dispelling pathogenic wind, removing toxic substance, regulating menstruation, and relieving pain.
More preferably, the minced blood-meat poultry in the step (1) comprises, by mass, 11.56-11.67 parts of black-bone chicken, 4.38-4.76 parts of rabbit, 3.40-3.89 parts of tortoise, 2.72-3.40 parts of crocodile and 2.03-2.92 parts of pigeon.
Further preferably, the mass ratio of the blood-meat poultry pieces to the water in the step (1) is 24.09: 75.91-26.64: 73.36. Still further preferably, the mass ratio of the blood-meat poultry pieces to the water is 24.98: 75.02.
preferably, the Chinese herbal medicine composite functional powder in the step (3) comprises the following components in parts by mass: 33.26 to 33.31 parts of hyacinth bean powder, 23.57 to 26.65 parts of yam powder, 18.86 to 20.79 parts of water chestnut powder, 6.66 to 7.07 parts of mulberry powder, 6.66 to 7.07 parts of medlar powder, 2.00 to 2.38 parts of American ginseng powder, 2.00 to 4.16 parts of schisandra powder, 0.50 to 0.94 part of ganoderma lucidum powder and 0.05 to 0.21 part of saffron crocus powder. Further preferably, the Chinese herbal medicine composite functional powder in the step (3) comprises the following components in parts by mass: 33.26 parts of hyacinth bean powder, 26.65 parts of yam powder, 20.79 parts of water chestnut powder, 6.66 parts of mulberry powder, 6.66 parts of medlar powder, 2.00 parts of American ginseng powder, 2.00 parts of Chinese magnoliavine fruit powder, 0.50 part of lucid ganoderma powder and 0.05 part of saffron powder.
The invention relates to a hyacinth bean: enter spleen and stomach meridians. Sweet and neutral. Invigorating spleen, regulating stomach, removing summer-heat, and eliminating dampness; chinese yam: spleen, lung and kidney meridians; sweet and neutral. Invigorating spleen and stomach, promoting fluid production, benefiting lung, invigorating kidney, and arresting seminal emission; water chestnut: enters lung and stomach meridians. Sweet and cold. Clearing heat, eliminating phlegm, and resolving food stagnation; and (3) mulberry fruit: entering the heart; the liver and kidney meridians. Sweet, sour and cold. Enriching blood, nourishing yin, promoting fluid production and moistening dryness; medlar: entering liver and kidney meridians; sweet and neutral. Nourishing liver and kidney, replenishing vital essence and improving eyesight; american ginseng: enter heart, lung and kidney meridians. Sweet, slightly bitter and cool. Invigorating qi, nourishing yin, clearing away heat, and promoting salivation; schisandra chinensis: entering lung, heart and kidney meridians; a monosodium glutamate; and (4) temperature. Astringing, invigorating qi, promoting fluid production, calming heart, and tranquilizing; ganoderma lucidum: it enters heart, lung, liver and kidney meridians. Sweet and neutral. Invigorating qi, tranquilizing mind, relieving cough and asthma; saffron: entering the heart; the liver meridian. Sweet; and (7) flattening. Promoting blood circulation, removing blood stasis, resolving stagnation, cooling blood, and removing toxic substance.
Preferably, the mass ratio of the nutrient solution to the Chinese herbal medicine composite functional powder is 73.55-84.99: 15.01-26.45. Further preferably, the mass ratio of the nutrient solution to the Chinese herbal medicine composite functional powder is 78.79: 21.21.
Preferably, the particle size of each component in the Chinese herbal medicine composite functional powder is 60 meshes.
Preferably, the auxiliary material in the step (2) is potassium sorbate. The adding amount of the potassium sorbate is 0.75 to 0.78 percent of the mass of the nutrient solution.
The invention also protects the qi and yin deficiency health-care capsule food prepared by the method.
Compared with the prior art, the invention has the beneficial effects that:
1. the invention does not use chemical preparation in the preparation process, and ensures that the nutrient components of the raw materials can be fully reserved. The formula components are mainly prepared by extracting high-quality protein nutrient solution from various blood meat poultry animals and compounding with medical and edible Chinese herbal medicines, and the formula uses cool American ginseng, mulberry, water chestnut powder, medlar and lucid ganoderma with mild nature to supplement qi and nourish yin, calm the nerves and moisten dryness, the sour schisandra fruit astringes and fixes, hyacinth beans with mild nature and Chinese yam tonify spleen and supplement qi, and saffron with less adjuvant drugs can activate blood and dispel depression. Meanwhile, rabbit meat and crocodile meat with cold nature, pigeons meat and black-bone chicken meat with warm nature are selected as main materials, and turtle meat with warm nature is added to achieve the effects of tonifying qi and blood, nourishing liver and kidney and strengthening bones and muscles.
2. For people with constitution of qi and yin deficiency, for example, people who are often staying up all night, eating food which is easy to get inflamed, and sexual life times are too frequent with the increase of age, and chronic consumptive diseases and the like can cause the symptoms of weakness, qi deficiency, talk reluctance, body weakness, dryness, soreness and pain of waist and knees, dysphoria with smothery sensation in chest, dizziness, tinnitus, dry mouth and tongue and the like of patients with qi and yin deficiency. All have obvious improvement effect, can be used as a health-care functional food product for people with constitution of deficiency of qi and yin to eat at ease.
3. The capsule food produced by the preparation method provided by the invention can better extract and retain nutrient components, has the functions of tonifying qi and yin, nourishing liver and kidney, promoting blood circulation and soothing nerves and has the health-care effect of enhancing the immunity of a human body aiming at sub-health caused by deficiency of qi and yin and the physique of people needing to be well-maintained.
Drawings
FIG. 1 shows the change of organs of mice in each treatment group in Experimental example 1, wherein the group of yin-deficiency capsules refers to the group of high dosage of yin-deficiency capsules.
Detailed Description
The following examples are further illustrative of the present invention and are not intended to be limiting thereof. Experimental procedures without specific conditions noted in the following examples, generally according to conditions conventional in the art or as recommended by the manufacturer; the raw materials, reagents and the like used are, unless otherwise specified, regarded as raw materials and reagents that are commercially available through conventional markets and the like. Any insubstantial changes and substitutions made by those skilled in the art based on the present invention are intended to be covered by the claims.
Example 1:
a method for preparing qi and yin deficiency health capsule food by adopting high-quality protein extracted from blood meat poultry and Chinese herbal medicines comprises the following steps:
(1) selecting qualified quarantine materials, slaughtering gallus Domesticus, rabbit, Testudinis, crocodile, and pigeon with dehairing, head removing, and viscera removing effects in biological laboratory or special production and management place meeting food safety standard, cleaning, and chopping;
(2) adding the blood-meat poultry treated in the step (1) into a multifunctional extraction tank according to the proportion that the total mass fraction of the blood-meat poultry and clear water is 100%, 11.56% of silky fowl, 4.38% of rabbit, 3.40% of tortoise, 2.72% of crocodile, 2.92% of pigeon and the balance of clear water, and heating and extracting under the pressure of 0.1 Mpa;
(3) opening a condensing valve when the temperature of the extraction liquid reaches 105 ℃ until the surface liquid is distilled, filtering the residual materials in the extraction tank, and taking filtrate;
(4) slowly adding potassium sorbate with the mass of 0.76% of the filtrate into the filtrate obtained in the step (3), and uniformly stirring to obtain a high-quality protein nutrient solution extracted from the blood-flesh poultry animal;
(5) adding 60-mesh Chinese herbal medicine composite functional powder consisting of 33.26 parts of hyacinth bean powder, 26.65 parts of yam powder, 20.79 parts of water chestnut powder, 6.66 parts of mulberry powder, 6.66 parts of medlar powder, 2.00 parts of American ginseng powder, 2.00 parts of schisandra powder, 0.50 part of lucid ganoderma powder and 0.05 part of saffron powder into the nutrient solution obtained in the step (4) according to the mass ratio of 78.79:21.21, uniformly stirring the two, performing spray drying, filling capsules according to 0.5 g/capsule, and filling to obtain the qi-yin-deficiency health capsule food.
Comparative example 1
The preparation method of the capsule food 1 comprises the following steps:
(1) selecting qualified quarantine materials, slaughtering gallus Domesticus, rabbit, Testudinis, crocodile, and pigeon, which have depilated, head removed, and viscera removed by biological laboratory or special production place meeting food safety standard, cleaning, and cutting;
(2) adding the blood meat poultry processed in the step (1) into a multifunctional extraction tank according to the proportion that the total mass fraction of the blood meat poultry and clear water is 100%, 11.56% of black-bone chicken, 4.38% of rabbits, 3.40% of tortoise, 2.72% of crocodile, 2.92% of pigeon and the balance of clear water, and heating and extracting under the pressure of 0.1 MPa;
(3) opening a condensing valve when the temperature of the extract liquid reaches 105 ℃ until the surface liquid is distilled, filtering the residual materials in the extraction tank, and taking the filtrate to obtain the high-quality protein nutrient solution extracted from the blood-flesh poultry;
(4) and (4) carrying out spray drying on the nutrient solution obtained in the step (3), filling capsules according to 0.5 g/capsule, and filling to obtain the capsule food 1.
Firstly, the capsule food 1 obtained in the comparative example 1 is tested, and the content of effective nutrient components is shown in the table 1:
TABLE 1 effective nutrient content
| Item | Content (per 100g) |
| Protein | 96.31 |
| Polysaccharides | 0.94 |
| Total saponins | 0.00 |
| Total triterpenes | 0.00 |
| Crocin I + crocin II | 0.00 |
Secondly, as a food, 100 volunteers with yin deficiency took 12g per day (three meals a day, 8 granules were taken half an hour after each meal) without the assistance of other medicines, 1% of the volunteers had a reduction in the symptoms of yin deficiency after eating for 7 days, 8% of the volunteers had a reduction in the symptoms of yin deficiency after eating for 10 days, 15% of the volunteers had a reduction in the symptoms of yin deficiency after eating for 15 days, and 38% of the volunteers had a reduction in the symptoms of yin deficiency after eating for 25 days.
Comparative example 2
The preparation method of the capsule food 2 comprises the following steps: 33.26 parts of 60-mesh hyacinth bean powder, 26.65 parts of yam powder, 20.79 parts of water chestnut powder, 6.66 parts of mulberry powder, 6.66 parts of medlar powder, 2.00 parts of American ginseng powder, 2.00 parts of schisandra powder, 0.50 part of ganoderma lucidum powder and 0.05 part of saffron powder are mixed and stirred uniformly, and the mixture is filled into capsules according to 0.5 g/capsule and filled to obtain the capsule food 2.
Firstly, when the capsule food 2 obtained in the comparative example 2 is tested, the content of effective nutrient components is shown in the table 2:
TABLE 2 effective nutrient content
| Item | Content (per 100g) |
| Protein | 46.45 |
| Polysaccharides | 8.98 |
| Total saponins | 4.33 |
| Total triterpenes | 0.78 |
| Crocin I + crocin II | 0.10 |
And thirdly, as a food, 100 volunteers with yin deficiency take 12g (three meals a day, 8 granules are taken half an hour after each meal) every day without the assistance of other medicines, 10% of the volunteers have relieved symptoms of yin deficiency after eating for 7 days, 28% of the volunteers have relieved symptoms of yin deficiency after eating for 10 days, 48% of the volunteers have relieved symptoms of yin deficiency after eating for 15 days, and 63% of the volunteers have relieved symptoms of yin deficiency after eating for 25 days.
Example 2
A method for preparing qi and yin deficiency health-care capsule food by adopting high-quality protein extracted from blood meat poultry and Chinese herbal medicines comprises the following steps:
(1) selecting qualified quarantine materials, slaughtering gallus Domesticus, rabbit, Testudinis, crocodile, and pigeon with dehairing, head removing, and viscera removing effects in biological laboratory or special production and management place meeting food safety standard, cleaning, and chopping;
(2) adding the blood-meat poultry treated in the step (1) into a multifunctional extraction tank according to the proportion that the total mass fraction of the blood-meat poultry and clear water is 100%, 11.67% of silky fowl, 4.76% of rabbit, 3.89% of tortoise, 3.40% of crocodile, 2.03% of pigeon and the balance of clear water, and heating and extracting under the pressure of 0.1 Mpa;
(3) opening a condensing valve when the temperature of the extraction liquid reaches 105 ℃ until the surface liquid is distilled, filtering the residual materials in the extraction tank, and taking filtrate;
(4) slowly adding potassium sorbate with the mass of 0.78% of the filtrate into the filtrate obtained in the step (3), and uniformly stirring to obtain a high-quality protein nutrient solution extracted from the blood-meat poultry animal;
(5) adding 60-mesh Chinese herbal medicine composite functional powder consisting of 33.31 parts of hyacinth bean powder, 23.57 parts of yam powder, 18.86 parts of water chestnut powder, 7.07 parts of mulberry powder, 7.07 parts of medlar powder, 2.38 parts of American ginseng powder, 4.16 parts of schisandra powder, 0.94 part of ganoderma lucidum powder and 0.21 part of saffron crocus powder into the nutrient solution obtained in the step (4) according to the mass ratio of 73.55:26.45, uniformly stirring the two, performing spray drying, filling capsules according to 0.5 g/capsule, and filling to obtain the qi-yin-deficiency health capsule food.
Experimental example 1
Firstly, the capsules for recovering from deficiency of qi and yin obtained in example 1 are tested, and the contents of effective nutrient components are shown in table 3:
TABLE 3 effective nutrient content
| Item | Content (per 100g) |
| Protein | 87.62 |
| Polysaccharides | 3.38 |
| Total saponins | 1.15 |
| Total triterpenes | 0.23 |
| Crocin I + crocin II | 0.03 |
Secondly, as a food, 100 volunteers with yin deficiency took 12g per day (three meals a day, 8 granules are taken half an hour after each meal) without the assistance of other medicines, 35% of the volunteers took the food for 7 days to relieve the symptoms of yin deficiency, 52% of the volunteers took the food for 10 days to relieve the symptoms of yin deficiency, 83% of the volunteers took the food for 15 days to relieve the symptoms of yin deficiency, and 96% of the volunteers took the food for 25 days to relieve the symptoms of yin deficiency.
Acute toxicity study of qi-yin-deficiency-recovering capsule (yin-deficiency capsule)
1. Experiment grouping
The test article is administered several times a day by maximum dose method. 40 ICR mice were divided into different dosing groups and a control group, each group containing 10 animals. The control group was given the same volume of distilled water as the administration group. The specific experimental animal groups and gavage rates are shown in table 4. The yin-deficiency capsule obtained in example 1 is dissolved in water to form a solution with a mass concentration of 0.3 g/mL.
TABLE 4 grouping and gavage of experimental animals
| Group of | Number of animals | Number of administrations | Volume of administration |
| Control group | 10 | 3 | 0.5mL × 3 times |
| High dose capsule for yin deficiency | 10 | 3 | 0.5mL × 3 times |
| Yin deficiency capsule middle dose group | 10 | 2 | 0.5mL × 2 times |
| Low dosage capsule for treating yin deficiency | 10 | 1 | 0.5mL × 1 time |
2. Experimental observation and analysis
After the yin-deficiency capsules were dissolved in water (0.3g/mL), the administration was completed in accordance with the gavage amount of Table 4, followed by continuous observation for 14 days, and the reaction of the animals was closely observed within 48 hours after the administration. According to the requirements of relevant content of the technical guidance principle of acute toxicity research of traditional Chinese medicines and natural medicines, the toxic reaction condition of animals, toxic symptoms, the occurrence time, duration, recovery time, the shortest death time, the longest death time, the average death time and the number of dead animals are recorded in detail, the dead animals are dissected and necropsied in time, wherein abnormal animals are observed by naked eyes for pathological histological examination, and the recording is not limited to the following contents:
the appearance of the animal: whether the skin color is changed, whether the skin has a red and swollen phenomenon, whether spots appear, whether the hair is glossy, and the like.
② the nervous system: behaviors and responses, abnormal movements, responses to stimuli, neuroreflexes, tics, muscle tone.
③ breathing function: depth and frequency of breathing, nasal secretions, wheezing, and whether tachypnea or apnea is present.
Fourthly, the exercise function: the gait, increase or decrease of movement, abnormal movement, prone position, tremor, fasciculation, etc. of the mice are observed.
The reflection function: corneal eyelid closure, orthostatic reflectance, stretch reflectance, light reflectance were examined.
Indications of the eye: such as exophthalmos, ptosis, conjunctival opacity, iritis, conjunctivitis.
Seventhly, cardiovascular indications: and (5) expanding blood vessels.
Eighthly, gastrointestinal indications: stool characteristics, abdominal morphology, etc.
Ninthly, indication of the urogenital system: external genital conditions.
At the end of the observation period, the surviving animals are weighed and then sacrificed and subjected to anatomical examination, the changes of organs such as heart, liver, kidney, spleen, lung, stomach and intestine are observed, if the changes are obvious, pathological examination is further carried out, the weight of each organ is counted, and the organ coefficient is recorded.
3. Results of the experiment
After the mice receive the yin-deficiency capsules with different doses and are subjected to intragastric administration for 14 days, compared with a control group, the mice have normal appearance and no death phenomenon, and the appearance, the action and the body posture and the appearance and the state of the mice have no obvious abnormality, which indicates that the LD50 of the yin-deficiency capsules is greater than the maximum tolerance dose of the mice to the mice and has no obvious acute toxicity.
The maximum dose of the mouse is about 87.5 times of the normal dose (1.2g/70kg) for an adult in terms of 1.5g/kg body weight, and the mouse shows good safety.
Because the test sample has no obvious toxicity, in order to make the experimental result simple and visual, only the normal control group and the high-dose experimental group of the yin-deficiency capsule are analyzed subsequently.
(1) Appearance observation of experimental animal
Before and after administration, the animals have no obvious abnormality in diet and activity, and the appearance of excreta is normal. No animal death was observed throughout the experiment. Through continuous observation, the appearance of the mice before and after administration is not obviously abnormal, the mind is clear, the action gait is steady, the hair color is bright, and the phenomena of salivation and nasal discharge (tables 5 and 6) do not occur.
TABLE 5 observations and recordings of experimental animals before experiment
Note: "-" indicates that the physiological indication depicted in the graph is negative, "+" indicates that the physiological indication depicted in the graph is positive, and the number of the row corresponding to "+" is 0, indicating that no animal is experiencing an abnormal indication depicted in the graph.
TABLE 6 acute toxicity of animals after administration
Note: the "+" indicates that the physiological indicators depicted in the graph, such as abnormal muscle movements, are positive, the "-" indicates that the physiological indicators depicted in the graph are negative, and the "+" indicates that no animals were shuffled and the abnormal indicators depicted in the graph are 0. "+" this indicator is short lived, probably due to mechanical physical stimulation during the gavage procedure, non-drug induced.
(2) Mice body weight change before and after dosing was recorded
The mice were weighed at different time points, and it was found that the weight change of the yin-deficiency capsule-administered mice after receiving the gavage treatment was substantially identical to that of the control group, and the weight of the yin-deficiency capsule-administered mice was significantly higher than that of the control group on days 11 and 14 of administration (table 7).
TABLE 7 weight changes before and after administration of mice
Data are shown to be significantly different (P < 0.05) compared to control.
(3) Recording the visceral changes of the experimental animals after the gavage treatment
After the experiment, the animals of the administration group and the control group were roughly dissected, and no significant abnormal change in organs such as heart, lung, liver, spleen, kidney, and stomach and intestine was observed in each group of mice (see tables 8 and 9). Weighing partial organs such as liver, spleen, lung, kidney and reproductive organs, calculating related organ coefficients (organ weight/body weight), wherein the index of main organs such as heart, lung, liver and kidney has no significant difference when the yin deficiency capsule administration treatment group (yin deficiency capsule high dose group) is compared with the control group, and interestingly, the index of spleen and thymus of mice in the yin deficiency capsule group is significantly increased, so that the yin deficiency capsule can promote the growth of immune organs of the mice and improve the immunity of the mice.
TABLE 8 mouse organ Change
TABLE 9 mouse organ index
Data are shown to be significantly different (P < 0.05) compared to control.
(4) Pathological examination of mouse organ
Part of visceral organs of each group of mice are respectively taken, and tissue sections are taken for histopathological examination, obvious abnormal lesions are not found in the visceral organs of the mice of each treatment group, as shown in figure 1, the visceral organs have uniform texture, clear nuclear texture edges, regular shapes and no tissue necrosis, and the liver and spleen tissues of the mice of each group have complete forms and no obvious abnormal structures.
5. Conclusion
After the mice are subjected to continuous observation for 14 days after the mice are subjected to intragastric administration of yin-deficiency capsules with different doses within one day, no abnormal reaction of animals occurs in the whole observation period, and no animal death occurs. The weight detection result shows that the weight of the mice is obviously higher than that of the control group after the yin deficiency capsule is administrated for intragastric administration for one week. Through tissue observation and histopathological examination, the yin-deficiency capsule is not found to have obvious adverse effect on main organs of mice, but can obviously promote the increase of the spleen and thymus gland mass.
The results show that the yin-deficiency capsule has no obvious acute toxicity to experimental mice and has obvious growth promoting effect on main immune organs of the mice.
Experimental example 2 therapeutic effect of yin deficiency capsule on kidney yin deficiency mice
Taking 20 ICR mice of 6-7 weeks old, dividing into a control group and a yin-deficiency capsule administration group, dissolving the yin-deficiency capsule in water, administering to the mice by gastric lavage once a day for 21 days continuously, 1 hour after each administration, injecting 25% India ink 20mL/kg into tail vein of the mice, collecting blood 50 μ L from orbital venous plexus of the mice after 1 minute and 5 minutes after injection, dissolving in 3mL of 0.1% Na 2 CO 3 Shaking the solution evenly, standing for 30 minutes, measuring OD value at 680nm on an ultraviolet spectrophotometer, and adding 0.1% Na 2 CO 3 The solution is blank control; taking liver and spleen of mouse after the mouse dies at cervical vertebra, sucking dry blood with filter paper, weighing, and calculating clearance index and phagocytosis index per unit time according to the following calculation formula
The clearance index is (lgOD 1-lgOD 5)/(t 5-t 1);
phagocytosis index (α) ═ K × body weight/(liver weight + spleen weight);
and (5) counting the K and alpha values of the drug group and the control group, and performing a t value test. The test data were analyzed using SPSS software, and the results are shown in Table 10.
TABLE 10 mouse macrophage clearance index and phagocytosis index
Indicates significant differences compared to the blank control (P < 0.01).
From the above results, it can be seen that, after the administration of the liquid medicine of example 1 of the present invention to the stomach of a patient for 21 days, the clearance index and the phagocytic index of the mouse are significantly improved, and the difference is significant (P is less than 0.01), that is, the yin deficiency capsule can significantly improve the phagocytic capacity of the mononuclear-macrophage, which indicates that the yin deficiency capsule significantly improves the immunity of the body.
Experimental example 3 Effect of yin deficiency capsules on kidney yin deficiency mice
The main research contents are as follows
Healthy Kunming mice were 30, 5-6 weeks old, and after one week of acclimation, the mice were randomly divided into 3 groups of 10 mice each. The specific grouping is as follows:
control group, i.e. intraperitoneal injection
Physiological saline 0.3mL/1 times/day for 7 consecutive days.
Kidney yin deficiency group: intraperitoneal injection of hydrocortisone (50 mg/(kg body weight. day)) for 7 consecutive days;
treatment group for deficiency of kidney yin: the abdominal cavity is injected with hydrocortisone 50mg/(kg day), and the dosage of the oral enema stomach yin deficiency capsule solution is 10mL/kg body weight for 7 days continuously.
During the experiment period, the mice drink water and diet freely, the appearance forms and the activity states of the mice are observed every day, relevant records are made, and after the 7-day experiment treatment period is finished, the blood of the mice is collected to carry out relevant biochemical and biological index detection.
The experimental results are as follows:
(1) morphological and appearance Observation
The appearance and behavior of the mice in the normal group are normal, the mice show bright hair color, active movement, normal diet and moist and granular excrement. The kidney yin deficiency mice have the phenomena of fluffy hair, inactive behaviors, irritability, bite, poor mental state, loose stool and frequent hunch back. After the stomach is perfused with the yin-deficiency capsule, the hair color of the mouse becomes bright, the activity of the mouse is increased, and the excrement is granular.
(2) Biochemical detection
By detecting the levels of FSH, testosterone and estrogen E2 in the serum of each group of mice, the serum of the mice with deficiency of kidney yin is obviously lower than that of a normal control group (P is less than 0.05), but the level of estrogen is not obviously changed. After the yin deficiency capsule treatment, the testosterone level is obviously increased, the value of the testosterone level is not obviously different from that of a normal group, the FSH level is also obviously increased, but the difference is still larger compared with that of the normal group; the yin deficiency capsule also reduces the level of estrogen in the serum of the kidney yin deficiency mice to a certain extent, but the difference is not obvious, and the specific experimental results are shown in table 11.
TABLE 11 levels of FSH, testosterone and Estrogen E2 in mouse serum
a shows that compared with a normal control group, P is less than 0.05, and the difference is significant;
b shows that compared with the group with deficiency of kidney yin, P is less than 0.05, and the result has significant difference.
Experimental example 4 Effect of yin deficiency capsules on qi and yin deficiency population
According to the guidelines (trial) of clinical research on new Chinese medicines, 60 candidates are selected in combination with the syndrome differentiation standard of traditional Chinese medicine, and the inclusion standard includes the Chinese medicine syndrome differentiation standard of qi and yin deficiency, the age of 40-78 years, informed consent of the group members and the like. The group-added people are subjected to traditional Chinese medicine syndrome differentiation by a single blind method, and are classified into 6 grades according to symptom severity and scoring statistics, wherein the grades are according to asymptomatic symptoms (0 score), light symptoms (1 score), light symptoms (2 score), medium symptoms (3 score), heavy symptoms (4 score) and severe symptoms (5 score).
The group members take blood again on an empty stomach, obtain serum samples, detect related biochemical indexes TNF alpha, IL-1, IFN gamma and other cytokines, and the group members take the capsules for oral administration for two weeks before the detection and recording related data.
The experimental results are as follows:
according to the inclusion criteria, 46 persons were finally included in the study cohort, wherein serum samples from 21 persons were obtained for the detection of the relevant biochemical factors, and the results are shown in table 12.
TABLE 12 detection results of qi and yin deficiency groups
a shows that P is less than 0.05 compared with the data before treatment, and the data result has significant difference.
As can be seen from Table 12, after taking the capsule for treating yin deficiency, the symptoms of the people with qi and yin deficiency are remarkably relieved, mainly manifested by the symptoms of palpitation and shortness of breath, spontaneous perspiration, night sweat, dry mouth, and the symptoms of face, lip and tongue are obviously improved, and the pulse is stable. The level of each inflammatory factor is obviously reduced.
The results show that the yin-deficiency capsules have obvious effect of improving the symptoms of qi-yin deficiency people.
The above is only a preferred embodiment of the present invention, and it should be noted that the above preferred embodiment should not be considered as limiting the present invention, and the protection scope of the present invention should be subject to the scope defined by the claims. It will be apparent to those skilled in the art that various modifications and adaptations can be made without departing from the spirit and scope of the invention, and these modifications and adaptations should be considered within the scope of the invention.
Claims (10)
1. A method for preparing qi and yin deficiency health-care capsule food by selecting and extracting high-quality protein from blood meat poultry and Chinese herbal medicines is characterized by comprising the following steps:
(1) sequentially adding minced blood meat poultry and water into an extraction container, heating for extraction, opening a condensation valve when the temperature of an extraction liquid reaches 105 ℃, till the surface liquid is distilled, and filtering to obtain a filtrate for later use;
(2) adding adjuvants into the filtrate, and stirring to obtain high quality protein nutrient solution extracted from blood meat poultry animal;
(3) and (3) adding the Chinese herbal medicine compound functional powder into the nutrient solution obtained in the step (2), uniformly stirring, and collecting after spray drying to obtain the qi and yin deficiency health-care capsule food.
2. The method according to claim 1, wherein the blood-fleshed poultry pieces in step (1) are obtained by: slaughtering poultry to remove hair, removing head and viscera to obtain blood meat poultry, cleaning, and chopping to obtain blood meat poultry pieces.
3. The method according to claim 1, wherein the poultry of step (1) comprises silkworms, rabbits, turtles, crocodiles and pigeons.
4. The method according to claim 3, wherein the blood meat poultry pieces in the step (1) comprise 11.56-11.67 parts by mass of black-bone chickens, 4.38-4.76 parts by mass of rabbits, 3.40-3.89 parts by mass of turtles, 2.72-3.40 parts by mass of crocodiles and 2.03-2.92 parts by mass of pigeons.
5. The method according to claim 1 or 3, wherein the mass ratio of the blood-meat poultry pieces to the water in the step (1) is 24.09: 75.91-26.64: 73.36.
6. The method according to any one of claims 1 to 5, wherein the Chinese herbal medicine composite functional powder in the step (3) comprises the following components in parts by mass: 33.26 to 33.31 parts of hyacinth bean powder, 23.57 to 26.65 parts of yam powder, 18.86 to 20.79 parts of water chestnut powder, 6.66 to 7.07 parts of mulberry powder, 6.66 to 7.07 parts of medlar powder, 2.00 to 2.38 parts of American ginseng powder, 2.00 to 4.16 parts of schisandra powder, 0.50 to 0.94 part of lucid ganoderma powder and 0.05 to 0.21 part of saffron powder.
7. The method according to claim 1 or 6, wherein the mass ratio of the nutrient solution to the Chinese herbal medicine composite functional powder is 73.55-84.99: 15.01-26.45.
8. The method as claimed in claim 1 or 6, wherein the particle size of each component of the herbal composite functional powder is 60 mesh.
9. The method according to claim 1 or 6, characterized in that the auxiliary material in step (2) is potassium sorbate, and the addition amount of potassium sorbate is 0.75-0.78% of the mass of the filtrate.
10. The qi and yin deficiency health preserving capsule food prepared by the method of claim 1.
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| CN107125751A (en) * | 2017-05-27 | 2017-09-05 | 杨胜淼 | Tortoise powder, preparation method and applications |
| CN107307423A (en) * | 2017-07-12 | 2017-11-03 | 吉林青晨药业有限公司 | Product and preparation method with regulation enhancing body's immunity |
| CN108403931A (en) * | 2018-05-15 | 2018-08-17 | 江西益佰年药业股份有限公司 | A kind of black chicken oral liquid and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107125751A (en) * | 2017-05-27 | 2017-09-05 | 杨胜淼 | Tortoise powder, preparation method and applications |
| CN107307423A (en) * | 2017-07-12 | 2017-11-03 | 吉林青晨药业有限公司 | Product and preparation method with regulation enhancing body's immunity |
| CN108403931A (en) * | 2018-05-15 | 2018-08-17 | 江西益佰年药业股份有限公司 | A kind of black chicken oral liquid and preparation method thereof |
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