CN114982760B - Method for measuring bait trapping rate of slow-eye muscadine larvae phagostimulant for Chinese chives - Google Patents
Method for measuring bait trapping rate of slow-eye muscadine larvae phagostimulant for Chinese chives Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
- A01N41/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom not containing sulfur-to-oxygen bonds, e.g. polysulfides
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/002—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits
- A01N25/006—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits insecticidal
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
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Abstract
The invention provides a method for measuring bait trapping rate of bradysia odoriphaga larva phagostimulant, and the composition is a combination of at least two of dimethyl trisulfide, methyl propyl trisulfide, methyl allyl trisulfide or 2-methyl-2-pentenal. The phagostimulant composition for the bradysia odoriphaga larvae can directly attract a considerable amount of bradysia odoriphaga larvae from the tubers of the leeks, obviously weakens the concealment of the bradysia odoriphaga mosquitoes, increases the exposure degree, lays a foundation for the effective action of medicament prevention and treatment measures, and can achieve the purposes of reducing and enhancing the efficiency and reducing the cost.
Description
Technical Field
The invention belongs to the field of agricultural pest control, and particularly relates to a method for measuring a phagostimulant and a bait trapping rate for bradysia odoriphaga larva of Chinese chives.
Background
The bradysia odoriphaga is also called a Chinese chive maggot, is a phytophagous insect, has rich diet, and is common on crops such as Chinese chives, shallots, garlic and the like, and the hosts are mostly generated on other plants such as lily, cucurbitaceae, cruciferae, umbelliferae, jersey and the like. The bradysia odoriphaga is only harmful in larva stage, and the clusters eat underground rhizome parts of plants, so that larva activities can be seen at the broken parts of the bradysia odoriphaga when the harmful is serious. The damaged plants are characterized by wilting, yellow leaves, thin seedlings, weak seedlings, death of the plants when serious, and lack of seedlings and broken ridges in the field.
In agricultural production, since the bradysia odoriphaga larvae are harmful and cryptic, a large amount of high-toxicity chemical pesticide is often required to be applied to control the insect pests. Therefore, the technology for attracting the bradysia odoriphaga larvae is researched, the hidden characteristic of the bradysia odoriphaga larvae for harm is weakened, the prevention and control efficacy of the medicament is improved, and the method is a requirement for modern agricultural development, in particular the requirement for organic production of the bradysia odoriphaga.
Disclosure of Invention
In view of the above, the present invention aims to overcome the defects in the prior art, and provides a method for determining the bait trapping rate and phagostimulant for the bradysia odoriphaga larvae.
In order to achieve the above purpose, the technical scheme of the invention is realized as follows:
a feeding promoting composition for the larva of bradysia odoriphaga comprises at least two of dimethyl trisulfide, methyl propyl trisulfide, methyl allyl trisulfide or 2-methyl-2-pentenal.
Preferably, the composition is a combination of methallyltrisulfide and at least one of dimethyl trisulfide, methylpropyltrisulfide or 2-methyl-2-pentenal.
Preferably, the composition is a combination of 2-methyl-2-pentenal and methallyl trisulfide, and the mass ratio of 2-methyl-2-pentenal to methallyl trisulfide is 1-8:1-6; the composition is a combination of dimethyl trisulfide, methyl propyl trisulfide and methyl allyl trisulfide, and the mass ratio of the dimethyl trisulfide, the methyl propyl trisulfide and the methyl allyl trisulfide is 0.1-4:0.1-4:0.1-6; the composition is a combination of dimethyl trisulfide, 2-methyl-2-pentenal and methyl allyl trisulfide, and the mass ratio of the dimethyl trisulfide to the 2-methyl-2-pentenal to the methyl allyl trisulfide is 0.1-4:0.1-8:0.1-6; the composition is a combination of methyl propyl trisulfide, 2-methyl-2-pentenal and methyl allyl trisulfide, and the mass ratio of the methyl propyl trisulfide to the 2-methyl-2-pentenal to the methyl allyl trisulfide is 0.1-4:0.1-8:0.1-6.
Preferably, the composition is a combination of dimethyl trisulfide, methyl propyl trisulfide, methyl allyl trisulfide and 2-methyl-2-pentenal; the mass ratio of the dimethyl trisulfide, the methyl propyl trisulfide, the methyl allyl trisulfide to the 2-methyl-2-pentenal is 0-10:0-10:0.1-12:0-20; preferably, the mass ratio of the dimethyl trisulfide, the methyl propyl trisulfide, the methyl allyl trisulfide and the 2-methyl-2-pentenal is 0-4:0-4:0.1-6:0-8; more preferably, the mass ratio of the dimethyl trisulfide, the methyl propyl trisulfide, the methyl allyl trisulfide and the 2-methyl-2-pentenal is 0-4:0-4:0.1-6:0.2-8; more preferably, the mass ratio of the dimethyl trisulfide, the methyl propyl trisulfide, the methyl allyl trisulfide and the 2-methyl-2-pentenal is 0-4:0.1-4:0.1-6:0-8.
More preferably, the mass ratio of the dimethyl trisulfide, the methyl propyl trisulfide, the methyl allyl trisulfide and the 2-methyl-2-pentenal is 1:1:1:2.
a phagostimulant for bradysia odoriphaga larvae, comprising a phagostimulant composition of bradysia odoriphaga larvae, wherein the composition accounts for 0.00001-1% of the total mass of the phagostimulant; preferably, the composition accounts for 0.0001-0.1% of the total weight of the phagostimulant; preferably, the composition comprises 0.0001-0.08% of the total weight of the phagostimulant.
A bait for the larvae of the bradysia odoriphaga of leeks is prepared from barley flour (100-300 parts), corn flour (120-280 parts), lentinan (1-5 parts) and phagostimulant (300-450 parts).
A method for measuring bait trapping rate of bradysia odoriphaga larva comprises the following steps:
(1) Placing 1 center table at the center of the vessel, uniformly placing a plurality of bait tables at the outer sides of the center tables, wherein the distances between the centers of the bait tables and the center of the center table are the same;
(2) Placing Chinese chives on a center table, inoculating larvae on the Chinese chives, covering soil, and compacting;
(3) Placing the baits with the same mass on each bait table, then covering soil, and compacting;
(4) Counting the number of larvae on each bait station after culturing, and calculating the trapping rate.
Further, the center table and the bait table in the step (1) have the same structure; the bait table comprises a rack and a net rack, and the net rack is fixed on the rack; the number of the bait stations is more than 2.
Further, the water content of the soil in the step (2) is the same as the water content of the soil in the step (3); the water content of the soil in the step (2) is 16-23%.
Further, the humidity of the culture step in the step (4) is 10-80%, the temperature is 10-35 ℃ and the time is 1-72 hours.
Compared with the prior art, the invention has the following advantages:
the phagostimulant composition for the bradysia odoriphaga larvae can directly attract a considerable amount of bradysia odoriphaga larvae from the tubers of the leeks, obviously weakens the concealment of the bradysia odoriphaga mosquitoes, increases the exposure degree, lays a foundation for the effective action of medicament prevention and treatment measures, and can achieve the purposes of reducing and enhancing the efficiency and reducing the cost.
According to the method for measuring the bait trapping rate of the bradysia odoriphaga larva, disclosed by the invention, a bait table is adopted for measurement, the grid-shaped net frame reduces the resistance of the larva close to the bait, and the bait trapping range is defined by the arrangement of the net frame, so that the bait trapping amount can be counted conveniently; the method is carried out in a soil environment with the humidity of 16-23% and the depth of 1-10cm, so that the moving capability of larvae in the soil is fully ensured, the light interference is eliminated, and the accurate expression of the phagostimulant trapping effect is ensured.
Drawings
Fig. 1 is a schematic view of a bait station according to an embodiment of the invention;
FIG. 2 is a schematic diagram showing the distribution of center stations and bait stations in a vessel according to the test method one of example 3 of the present invention;
FIG. 3 is a schematic diagram of a culture dish according to the second test method in example 3 of the present invention.
Reference numerals illustrate:
1. a bait station; 2. a stand; 3. a net rack; 4. a center table.
Detailed Description
Unless defined otherwise, technical terms used in the following examples have the same meaning as commonly understood by one of ordinary skill in the art to which the present invention pertains. The test reagents used in the following examples, unless otherwise specified, are all conventional biochemical reagents; the experimental methods are conventional methods unless otherwise specified.
The present invention will be described in detail with reference to examples.
Example 1 determination of response and selection coefficient of a feed composition for bradysia odoriphaga larvae of leeks
1. And assembling the Y-shaped insect olfactory instrument. The atmospheric sampling device QC-1B, an air purifying bottle (filled with granular activated carbon), an air humidifier (300 mL conical flask filled with deionized water) and a Y-shaped glass tube (three arms are 50 mm in length and 6 mm in diameter) are connected in sequence by using a silica gel hose (inner diameter 6 mm and outer diameter 8 mm). After the air tightness test was performed on the assembled "Y" type insect olfactory instrument, the air flow rate of the atmosphere sampler was set to 100 mL/min, and the ambient temperature was adjusted to 25 ℃.
2. 3-year-old bradysia odoriphaga larva is selected before the test, and subjected to 12 h hunger treatment. Before insect collection, soaking the fine hairbrush in distilled water, picking 5 insects, placing at about 1/2 position in the air outlet of the Y-shaped glass tube, dispersing the larvae, and lightly poking the tail of the larvae with the tip of the brush head to enable the larvae to enter a motion state. The insect test crawls against the wind and selects the odor source, when the 2/3 body of the larva enters the Y-shaped glass tube arm corresponding to the odor source, the larva starts to count time, and the larva stays for enough 2 min to be regarded as the selection.
3. The relative positions of the control bottle and the odor bottle were immediately exchanged after one group of 5 larvae was measured, and the "Y" type glass tube was exchanged. The amount of composition used in each set of experiments was 20 μl, and was applied dropwise to 350 mm ×200 mm rectangular filter papers using a pipette, and each set or more than 10 min completed, the filter papers were changed and re-dosed. The test uses fresh leeks as a control, the fresh leeks are ground into homogenate and smeared on filter paper with the same specification, and the fresh weight of 0.4 g leeks is determined to be optimal through pre-test. The method for preparing the bait composition for the bradysia odoriphaga larva with different proportions by diluting with 8% acetone solution is that 20000 times of the bait composition for the bradysia odoriphaga larva with different proportions is prepared, 10mL of each gradient diluent of each composition is prepared, and the preparation method is as follows: firstly, a pipette is used for sucking medicines according to the mixing ratio of the table 1, 1:1, respectively sucking 0.05 mu L of each medicament; 3:6, mixing and proportioning, wherein each medicament respectively absorbs 0.15 mu L and 0.3 mu L;3:11, and each medicine is respectively sucked into 0.15 mu L and 0.55 mu L. The sucked medicament is dissolved in 0.8mL of acetone, uniformly shaken, distilled water is added into the liquid medicament to reach the volume of 10mL, and the liquid medicament is fully and uniformly shaken to prepare the phagostimulant. Sealing for standby. In the test for attracting each composition, 4 groups of 20 heads were measured for each dilution gradient of each phagostimulant. Each time a phagostimulant was tested, a new odor bottle was replaced. The proportions and results of the compositions are shown in Table 1.
The measurement results calculate the selective reaction rate and the selective coefficient, and the formula is as follows:
note that: the selection coefficient is larger than 0, which indicates that the insect has a tendency to the odor source substances, the larger the numerical value is, the stronger the tendency is, and the maximum value is 1; and if the selection coefficient is smaller than zero and is a negative value, the test insects have negative tendency to the taste source.
TABLE 1 selection coefficient of Multimixed leek, hypsizygus marmoreus larva feeding composition
As can be seen from Table 1, the blending ratio of compositions B to C to compositions H to K was 1:1/1:1:1/1:1:1:1 or 3:6/3:3:6/3:3:3: at 6 (except 3:3:6:3 mix ratio of composition K), the selection coefficients were positive, indicating that in this mix ratio example, the bradysia odoriphaga larvae of leek had positive tropism for them, and at 1:1/1:1:1/1:1:1: the selection coefficients under the mixing ratio of 1 are all larger than 3:6/3:3:6/3:3:3:6 selection coefficients under the mixing ratio.
The selection coefficients of the composition A and the compositions D-G are negative in all three mixing proportion examples, which shows that the bradysia odoriphaga larvae have negative tropism for the bradysia odoriphaga larvae in the mixing proportion examples. However, at 3:6/3:3:6/3:3:3:6, the selection coefficient is larger than other mixing proportion, and the ratio of ether to aldehyde is about 1:2. according to the above analysis, the ratio of the four agents can be further determined as the ratio of the mixture of the trisulfide and the aldehyde is set as 1:2.
example 2 determination of response Rate and selection coefficient of the phagostimulant for the bradysia odoriphaga larvae of leeks
1. 3-year-old bradysia odoriphaga larva is selected before the test, and subjected to 12 h hunger treatment. Before insect collection, soaking the fine hairbrush in distilled water, picking 5 insects, placing at about 1/2 position in the air outlet of the Y-shaped glass tube, dispersing the larvae, and lightly poking the tail of the larvae with the tip of the brush head to enable the larvae to enter a motion state. The insect test crawls against the wind and selects the odor source, when the 2/3 body of the larva enters the Y-shaped glass tube arm corresponding to the odor source, the larva starts to count time, and the larva stays for enough 2 min to be regarded as the selection.
2. Screening experiments were performed with 5 larvae as a group, and the relative positions of the control and odor vials were immediately exchanged and the "Y" glass tube was replaced. The amount of each test composition was 20 μl, and the samples were applied dropwise to 350 mm ×200 mm rectangular filter papers using a pipette, and each time one set or more than 10 min was completed, the filter papers were replaced and re-dosed. The test uses fresh leeks as a control, the fresh leeks are ground into homogenate and smeared on filter paper with the same specification, and the fresh weight of 0.4 g leeks is determined to be optimal through pre-test. The compositions were diluted with 8% acetone solution to obtain a phagostimulant, which was subjected to an attraction test from high dilution to low dilution, and each composition was subjected to 4 groups of dilution gradients, totaling 20 heads. Each time a composition is tested, a new scent bottle is replaced. Specific dilution and results are shown in tables 2-4. The calculation formula is the same as in example 1.
TABLE 2 selection coefficients of Tripterygium wilt larva phagostimulants with different dilution gradients
As can be seen from table 2, the distribution of the selection coefficients varies between 1250-160000 times the dilution gradient interval for the three compositions. In the gradient dilution range of the two substance compositions, the selection coefficient of 20000-160000 times of liquid is positive, the maximum value is reached at 20000 times of liquid, and the selection coefficient is 0.1 at 160000 times and approaches 0; the selection coefficients of the liquid in the low dilution range 1250-10000 times are all negative numbers, and the selection coefficient is-1 when the lowest dilution is 1250 times. In the gradient dilution range of the three material compositions, the selection coefficient of 2500-160000 times of liquid is positive, the selection coefficient in the 20000-80000 times dilution interval is above 0.8, but the selection coefficient is 0.25 towards 0 when the ratio is 160000 times; the selection coefficient of the low dilution interval is only 1250 times of liquid, and the selection coefficient is minus 0.35. In the gradient dilution range of the four substance compositions, the selection coefficient of only 20000 times of liquid is positive. The distribution characteristics of the selection coefficients of the 2, 3 and 4 substance compositions in the 1250-160000 gradient dilution range are combined, the 2500-80000 gradient dilution range is selected as the trapping effect test range of the phagostimulant, and when the concentration of the composition is 20000 times, the trapping effect of each composition is better.
Example 3 configuration of bait for Amaranthus sonchifolius larvae and measurement of trapping Capacity
Preparing bait: the food bait is prepared from barley, corn, lentinan and phagostimulant according to the mass ratio of 150:150:3:350, the food bait is prepared from barley, corn, lentinan and water according to the same proportion in a control group, and the trapping rate of the food bait is measured. Wherein the composition in the bait is shown in the following table.
Table 3 compositions in baits
Test method one: soil bait burying method
Selecting enough 3-year-old bradysia odoriphaga larva to a culture dish paved with wet filter paper, and starving 24-h for later use. Weighing 2 kg of dried soil, adding distilled water, respectively adjusting the water content of the soil to 16%, 20%, 23% and 26% and uniformly stirring for later use. Taking enough fresh leek stems, and cutting into 3 mm stem segments for later use.
A center table is arranged at the center of the 15 cm glass culture dish, and 1.2g leek stem segments are arranged on the center table. Inoculating 80 hunger treated 3-year old slow-growing Hypsizygus marmoreus larvae on the central table Chinese chives. 1.2g of bait is weighed and is in a sphere shape, the bait is placed in the center of a net surface of a plastic bait table, and a control bait is placed on the bait table at a symmetrical position. And finally covering the soil, slightly pressing the soil surface to keep the soil thickness consistent, covering a culture dish cover with the soil thickness of 2.5-cm, and placing the culture dish cover in a constant-temperature incubator with the humidity of 60% and the temperature of 25 ℃.5 kinds of food baits are tested, each food bait is repeatedly treated for three times in 4 soil humidity treatments, after 24 h, plastic ventilation buckles are taken out with soil, the number of larvae on each plastic ventilation buckle is counted, the trapping rate of the food baits under each soil humidity is calculated, and the trapping rate is calculated according to the following formula.
And a test method II: bare filter paper trapping method
A 12cm dish was prepared and wet filter paper was laid. Food bait 1.2, g was weighed, agglomerated into spheres and placed at the wet filter paper mark point, as shown in fig. 3, with the treatment group and control group placed symmetrically. 1.2g leek stem segments (3 mm long) are placed at the center of a central circle (d=3 cm), 20 heads of 24 h hunger-treated 3-instar larvae of the bradysia odoriphora chikungunya are inoculated, a culture dish cover is covered, and the culture dish cover is placed in a non-light constant-temperature incubator with the temperature of 25 ℃ and the humidity of 60%. Each dish was treated with one, and after repeating each treatment three times, the amount of larvae staying in each hatched portion was counted after 12. 12 h (larvae of the line were counted in the hatched area), and the trapping rate was calculated. The attraction rate of 5 food baits is measured by the same test method, and the attraction rate is calculated according to the following formula.
Table 4 bait trap rate for bradysia odoriphaga larvae of leeks
As is clear from Table 4, in the soil bait-burying test, the food baits had the highest trapping rate of the food baits to the bradysia odoriphaga larvae at 20% of the soil water content, and had the worst trapping effect at 16% of the soil water content, and the trapping rates were all 0. Each food bait contained the composition "dimethyl trisulfide: methyl propyl trisulfide: the food bait of the methallyl trisulfide has the best trapping effect, and the highest trapping rate reaches 42.50% when the water content of the soil is 20%. The treatment trapping rate of the soil with the water content of 23 percent is up to 30.15 percent. The treatment trapping effect of the soil with the water content of 26% is poor, and the highest trapping effect is only 15.40%; the soil water content is 16%, the trapping effect is worst, and the trapping rate of the food bait is 0. It can be seen that when the water content of the soil is 23%, the migration ability of the bradysia odoriphaga larvae in the soil is strongest, and the odor diffusion effect of the food bait in the soil is best. When the water content of the soil is low, the soil has a great blocking effect on the migration capability of the bradysia odoriphaga, so that the trapping rate of the food bait is 0. As the soil moisture content is higher, the rate of food bait attraction decreases, which is related to the influence of the ability of the scent of the food bait to diffuse in the high moisture content soil. Thus 20% soil moisture content was chosen as the moisture content standard for the test soil. In the filter paper bare trapping test, the trapping rate of each food bait is similar to the size difference trend of the trapping rate of the soil bait burying method, but the overall level is higher, and especially the treatment of lower trapping effect in the soil bait burying test is more obvious, for example, the components are that: the composition and components of the methallyltrisulfide are dimethyl trisulfide: methyl propyl trisulfide: 2-methyl-2-pentenal: methyl allyl trisulfide ".
Compared with the two test methods, the trapping rate of each bait under the water content of 20% of the soil is obviously lower than that of the bare trapping rate on the filter paper, and the trapping effect of each food bait is weakened. On the one hand, in the test of measuring the trapping rate by the soil bait burying method, the soil is used as a habitat of the bradysia odoriphaga larva, so that the diffusion effect of the odor in the soil can be hindered, and the movement capability of the bradysia odoriphaga larva in the soil is obviously influenced by the water content of the soil. The bare trapping on the wet filter paper eliminates the environmental resistance under natural conditions, and the trapping capacity of the bait is higher. On the other hand, the soil bait-burying method does not need other operations in the test process. In the filter paper bare trapping method, shading treatment is needed to eliminate light interference. The filter paper has limited water retention capacity, water needs to be periodically supplemented to ensure the movement capacity of larvae, and the operation is more complicated. Therefore, the soil bait burying method can better simulate the natural growth environment of the bradysia odoriphaga, and the measured trapping rate is more realistic. In addition, in the statistical method, the bare trapping of the filter paper needs to count the larvae on the filter paper with a larger range around the bait at a certain moment, so that the larvae in the wandering state are easy to count, and experimental errors can be increased. The soil bait burying method only counts the insect amount on the bait table, the volume of the bait table is smaller and is similar to that of food bait, and the representativeness of the data is more accurate than that of the filter paper bare trapping method.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the invention.
Claims (6)
1. A feeding promoting composition for bradysia odoriphaga larvae is characterized in that: the mass ratio of the composition is 1-3:1-6 methylpropyl trisulfide: methyl allyl trisulfide with the mass ratio of 1-6:1-3 of 2-methyl-2-pentenal: methyl allyl trisulfide with the mass ratio of 1-3:1-3:1-11 dimethyl trisulfide: methyl propyl trisulfide: methyl allyl trisulfide with the mass ratio of 1-3:1-3:1-6 dimethyl trisulfide: 2-methyl-2-pentenal: methyl allyl trisulfide with the mass ratio of 1-3:1-3:1-11 methylpropyl trisulfide: 2-methyl-2-pentenal: the methyl allyl trisulfide or mass ratio is 1:1:1:1, dimethyl trisulfide: methyl propyl trisulfide: 2-methyl-2-pentenal: methallyltrisulfide.
2. A phagostimulant for bradysia odoriphaga larvae of Chinese chives is characterized in that: the phagostimulant comprises the phagostimulant composition of the bradysia odoriphaga larva of the leek in claim 1, wherein the composition accounts for 0.0025-0.005% of the total mass of the phagostimulant.
3. A bait for bradysia odoriphaga larvae of chives, which is characterized in that: said bait comprising 100-300 parts of barley flour, 120-280 parts of corn flour, 1-5 parts of lentinan and 300-450 parts of said phagostimulant of claim 2.
4. A method for measuring bait trapping rate of bradysia odoriphaga larvae of Chinese chives is characterized by comprising the following steps: the method comprises the following steps:
(1) Placing 1 center table at the center of the vessel, uniformly placing a plurality of bait tables at the outer sides of the center tables, wherein the distances between the centers of the bait tables and the center of the center table are the same;
(2) Placing Chinese chives on a center table, inoculating larvae on the Chinese chives, covering soil, and compacting;
(3) Placing the baits in claim 3 with the same mass on each bait table, then covering soil, and compacting;
(4) Counting the number of larvae on each bait station after culturing, and calculating the trapping rate;
the water content of the soil in the step (2) is the same as that of the soil in the step (3); the water content of the soil in the step (2) is 20-23%.
5. The method for determining bait trap rate of bradysia odoriphaga larva according to claim 4, wherein: the center table and the bait table in the step (1) have the same structure; the bait table comprises a rack and a net rack, and the net rack is fixed on the rack; the number of the bait stations is more than 2.
6. The method for determining bait trap rate of bradysia odoriphaga larva according to claim 4, wherein: the humidity of the culture step in the step (4) is 10-80%, the temperature is 10-35 ℃ and the time is 1-72 hours.
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| CN202210528008.XA CN114982760B (en) | 2022-05-16 | 2022-05-16 | Method for measuring bait trapping rate of slow-eye muscadine larvae phagostimulant for Chinese chives |
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Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6201026B1 (en) * | 1999-03-09 | 2001-03-13 | The Regents Of The University Of California | Volatile aldehydes as pest control agents |
| CN101341934A (en) * | 2007-07-13 | 2009-01-14 | 东莞市盛唐化工有限公司 | Citrus fruit fly food attractant |
| CN102100230A (en) * | 2010-12-29 | 2011-06-22 | 华南农业大学 | New use of 2-methyl-2-pentenal serving as bactericide |
| CN103399126A (en) * | 2013-08-02 | 2013-11-20 | 山东农业大学 | Indoor bioassay method of bradysia odoriphaga larvae |
| CN103766342A (en) * | 2014-02-28 | 2014-05-07 | 广东省农业科学院果树研究所 | Insecticide stock solution and application thereof |
| CN104170814A (en) * | 2014-07-24 | 2014-12-03 | 青岛农业大学 | Monitoring and trap-killing technology for bradysia odoriphaga |
| CN107258789A (en) * | 2017-08-17 | 2017-10-20 | 浙江省农业科学院 | Attractant of Bradysia odoriphaga and preparation method and application |
| CN111296441A (en) * | 2020-03-16 | 2020-06-19 | 北京嘉景生物科技有限责任公司 | Core attractant for attracting locust and core attractant and application thereof |
| WO2020222296A1 (en) * | 2019-04-27 | 2020-11-05 | 株式会社アクト・フォ | Beverage, food composition, health food composition, oral composition and taste improver |
| CN111903676A (en) * | 2020-06-27 | 2020-11-10 | 内蒙古大学 | Attractant for pollinating insects, method for improving plant maturing rate and application thereof |
| CN113498795A (en) * | 2021-08-17 | 2021-10-15 | 北京中农绿安有机农业科技有限公司 | Preparation method of pure natural plant acaricide |
-
2022
- 2022-05-16 CN CN202210528008.XA patent/CN114982760B/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6201026B1 (en) * | 1999-03-09 | 2001-03-13 | The Regents Of The University Of California | Volatile aldehydes as pest control agents |
| CN101341934A (en) * | 2007-07-13 | 2009-01-14 | 东莞市盛唐化工有限公司 | Citrus fruit fly food attractant |
| CN102100230A (en) * | 2010-12-29 | 2011-06-22 | 华南农业大学 | New use of 2-methyl-2-pentenal serving as bactericide |
| CN103399126A (en) * | 2013-08-02 | 2013-11-20 | 山东农业大学 | Indoor bioassay method of bradysia odoriphaga larvae |
| CN103766342A (en) * | 2014-02-28 | 2014-05-07 | 广东省农业科学院果树研究所 | Insecticide stock solution and application thereof |
| CN104170814A (en) * | 2014-07-24 | 2014-12-03 | 青岛农业大学 | Monitoring and trap-killing technology for bradysia odoriphaga |
| CN107258789A (en) * | 2017-08-17 | 2017-10-20 | 浙江省农业科学院 | Attractant of Bradysia odoriphaga and preparation method and application |
| WO2020222296A1 (en) * | 2019-04-27 | 2020-11-05 | 株式会社アクト・フォ | Beverage, food composition, health food composition, oral composition and taste improver |
| CN111296441A (en) * | 2020-03-16 | 2020-06-19 | 北京嘉景生物科技有限责任公司 | Core attractant for attracting locust and core attractant and application thereof |
| CN111903676A (en) * | 2020-06-27 | 2020-11-10 | 内蒙古大学 | Attractant for pollinating insects, method for improving plant maturing rate and application thereof |
| CN113498795A (en) * | 2021-08-17 | 2021-10-15 | 北京中农绿安有机农业科技有限公司 | Preparation method of pure natural plant acaricide |
Non-Patent Citations (1)
| Title |
|---|
| 有机桃园天敌与蚜虫的互作关系及生态调控措施;董民;张顶武;杜相革;;植物保护学报(04);327-332 * |
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