CN111213677A - Diatomite-asparagus caterpillar nuclear polyhedrosis virus composition and preparation thereof - Google Patents
Diatomite-asparagus caterpillar nuclear polyhedrosis virus composition and preparation thereof Download PDFInfo
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- CN111213677A CN111213677A CN202010100929.7A CN202010100929A CN111213677A CN 111213677 A CN111213677 A CN 111213677A CN 202010100929 A CN202010100929 A CN 202010100929A CN 111213677 A CN111213677 A CN 111213677A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
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- A01N25/14—Powders or granules wettable
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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Abstract
The invention discloses a diatomite-spodoptera exigua nuclear polyhedrosis virus composition, which comprises raw materials of diatomite and spodoptera exigua nuclear polyhedrosis virus; the diatomite has a sawtooth structure. The composition combines the special appearance of diatomite with an environment-friendly pesticide, can greatly shorten the onset time of the spodoptera exigua nuclear polyhedrosis virus, has strong pertinence to specific insects, can be inherited through breeding behaviors in a population, and cannot cause drug resistance of pests.
Description
Technical Field
The invention relates to the field of agriculture and chemistry, in particular to a diatomite-beet armyworm nucleopolyhedrosis virus composition and a preparation thereof.
Background
The beet armyworm nuclear polyhedrosis virus can enter insect bodies through the mouth or wound of the insect, proliferate in the insect bodies and spread to other insects in the population, so that the pest population is implosively infected with virus diseases, and the quantity of the pests is further reduced. Because the pesticide is a biological preparation, the pesticide can not generate drug resistance to insects, has pertinence in the process of killing insects, is very safe to animals and plants except target pests, and is an environment-friendly pesticide suitable for popularization.
When the beet armyworm nuclear polyhedrosis virus is orally infected, the beet armyworm nuclear polyhedrosis virus is usually released in the intestinal tracts of insects, so the effect is slow, and the pests cannot be suppressed to damage crops when the beet armyworm nuclear polyhedrosis virus faces a large insect disaster; in addition, viruses are insoluble in water, and a uniformly dispersed system is difficult to form in water when the viruses are prepared into liquid medicine, so that the use effect is influenced, and the viruses are easily influenced by weather factors after being applied, so that the drug effect is further reduced.
Disclosure of Invention
In order to solve the above problems, a first aspect of the present invention provides a diatomaceous earth-spodoptera exigua nuclear polyhedrosis virus composition comprising a bulk drug of diatomaceous earth and spodoptera exigua nuclear polyhedrosis virus; the diatomite has a sawtooth structure.
As a preferable technical scheme, the spodoptera exigua nuclear polyhedrosis virus raw material is 2000 hundred million PIB/gram of spodoptera exigua nuclear polyhedrosis virus raw powder.
In a preferable embodiment, the weight ratio of the original drug of Spodoptera exigua nuclear polyhedrosis virus to the original drug of diatomaceous earth is (1: 40) - (400: 1).
In a second aspect, the invention provides the use of a diatomaceous earth-spodoptera exigua nucleopolyhedrosis virus composition as described above for the control of lepidopteran insects.
As a preferred technical scheme, the lepidoptera insects are selected from one or more of prodenia litura, asparagus caterpillar, tea geometrid and spodoptera frugiperda.
The third aspect of the invention provides diatomite-beet armyworm nuclear polyhedrosis virus wettable powder, which is prepared from the raw materials of the diatomite-beet armyworm nuclear polyhedrosis virus composition, a dispersing agent, a wetting agent, a synergist and a filler.
As a preferable technical scheme, the dispersing agent is selected from one or a mixture of more of HMK-D1003, HMK-D1005, HMK-806, Morwet D425, Morwet D450, Morwet D500, Morwet D110, Morwet EFW and Morwet IP.
As a preferred technical scheme, the synergist is monosaccharide or polysaccharide.
As a preferable technical scheme, the weight ratio of the dispersing agent, the wetting agent and the filler is (1-2): 1: (2-3).
The fourth aspect of the invention provides a preparation method of the diatomite-spodoptera exigua nucleopolyhedrosis virus wettable powder, which comprises the following steps: pulverizing and mixing the above raw materials except diatomaceous earth, adding diatomaceous earth, shaking, and stirring.
Has the advantages that: the invention provides a diatomite-asparagus caterpillar nuclear polyhedrosis virus composition, which utilizes the special appearance of diatomite in combination with an environment-friendly pesticide, can greatly shorten the onset time of the asparagus caterpillar nuclear polyhedrosis virus, has strong pertinence to specific insects, can be inherited through reproduction behaviors in a population, and cannot cause drug resistance of pests; the invention also provides the diatomite-beet armyworm nucleopolyhedrosis virus wettable powder which can be uniformly dispersed in water to form a stable system, has strong adaptability to different water qualities, can improve the binding force between a liquid medicine and the surface of a plant after being applied, prolongs the pesticide effect time, and has wide application prospect.
Drawings
To further illustrate the beneficial effects of a diatomaceous earth-beet armyworm nucleopolyhedrosis virus composition and its preparation provided in the present invention, the accompanying drawings are provided, it should be noted that the drawings provided in the present invention are only selected from individual examples in all drawings and are not intended to limit the claims, and all other corresponding maps obtained by the drawings provided in the present application should be considered within the protection scope of the present application.
FIG. 1 is an optical microscope photograph of diatomaceous earth having a bur structure according to the present invention.
Detailed Description
The invention will be further understood by reference to the following detailed description of preferred embodiments of the invention and the examples included therein. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. To the extent that a definition of a particular term disclosed in the prior art is inconsistent with any definitions provided herein, the definition of the term provided herein controls.
As used herein, a feature that does not define a singular or plural form is also intended to include a plural form of the feature unless the context clearly indicates otherwise. It will be further understood that the term "prepared from …," as used herein, is synonymous with "comprising," including, "comprising," "having," "including," and/or "containing," when used in this specification means that the recited composition, step, method, article, or device is present, but does not preclude the presence or addition of one or more other compositions, steps, methods, articles, or devices. Furthermore, the use of "preferred," "preferably," "more preferred," etc., when describing embodiments of the present application, is meant to refer to embodiments of the invention that may provide certain benefits, under certain circumstances. However, other embodiments may be preferred, under the same or other circumstances. In addition, the recitation of one or more preferred embodiments does not imply that other embodiments are not useful, nor is it intended to exclude other embodiments from the scope of the invention.
In order to solve the above problems, a first aspect of the present invention provides a diatomaceous earth-spodoptera exigua nuclear polyhedrosis virus composition comprising a bulk drug of diatomaceous earth and spodoptera exigua nuclear polyhedrosis virus; the diatomite has a sawtooth structure.
In the present invention, the term "composition" means that the components of the composition are packaged in combination or separately and used together when used. That is, in the present invention, the "diatomaceous earth-spodoptera exigua nuclear polyhedrosis virus" means that diatomaceous earth and spodoptera exigua nuclear polyhedrosis virus can be mixed and packaged together, and used together when in use; also comprises that the diatomite and the spodoptera exigua nucleopolyhedrosis virus are separately packaged and are mixed together according to a proportion when in use.
In some preferred embodiments, the spodoptera exigua nuclear polyhedrosis virus bulk drug is 2000 hundred million PIB per gram of spodoptera exigua nuclear polyhedrosis virus bulk powder.
The 2000 hundred million PIB/gram spodoptera exigua nuclear polyhedrosis virus raw powder in the present application was purchased from Cistanchis actual Co., Ltd, economic in Henan.
In some preferred embodiments, the weight ratio of the diatomite to the technical material of the spodoptera exigua nucleopolyhedrovirus is (1: 40) - (400: 1).
The diatomaceous earth in this application is manufactured by Yirui Stone, France, under the trade name Celite610, with serrations in its microstructure.
The beet armyworm nucleopolyhedrosis virus is a biological pesticide, only effective for specific population, safe for human beings or other animals and plants, and is an environment-friendly pesticide worth popularizing, and can make beet armyworm and its adjacent population infect by mouth or wound, and the virus can release baculovirus particles in the pest body, invade cell and multiply greatly until the insect dies, and the excrement and corpse of the disease and insect can continuously transmit virus, and can even spread the virus to progeny through egg transmission, so that large-scale virus disease can be outbreaked in the population, and the quantity of pest can be reduced. However, the virus enters the body of the insect orally, needs to be digested by the insect and enters the body cavity through midgut epithelial cells, and the process of proliferation also takes some time, so the effect is slow, and the virus needs to be used for a plurality of times in a specific period, otherwise the effect is not good in the outbreak of the insect pest.
The inventor unexpectedly discovers that the onset time of the insecticide can be greatly shortened and the drug effect can be prolonged when the diatomite with a specific structure is used in combination with the spodoptera exigua nuclear polyhedrosis virus, and the combination is characterized in that the diatomite with the sawtooth structure (shown in figure 1) has extremely high surface activity and can be adsorbed on the surface of an insect after contacting with the insect, sharp sawteeth cut the epidermis of the insect along with the movement of the insect to enable the body fluid of the insect to flow out, the spodoptera exigua nuclear polyhedrosis virus has an opportunity to enter the insect body through wounds, the process of releasing through a digestive system is omitted, the onset time is faster, the diatomite has extremely strong water absorption, a large amount of body fluid of the insect can be absorbed, the death rate is further accelerated, the insect finally turns into water to die, the probability of other insect infection in a population is increased, and the influence range of the insecticide is expanded, prolongs the drug effect time.
In a second aspect, the invention provides the use of a diatomaceous earth-spodoptera exigua nucleopolyhedrosis virus composition as described above for the control of lepidopteran insects.
As a preferred technical scheme, the lepidoptera insects are selected from one or more of prodenia litura, asparagus caterpillar, tea geometrid and spodoptera frugiperda.
The third aspect of the invention provides diatomite-beet armyworm nuclear polyhedrosis virus wettable powder, which is prepared from the raw materials of the diatomite-beet armyworm nuclear polyhedrosis virus composition, a dispersing agent, a wetting agent, a synergist and an adsorbent.
In some preferred embodiments, the raw materials for preparing the wettable powder comprise: 4-8 wt% of a dispersing agent, 3-7 wt% of a wetting agent, 3-7 wt% of a synergist, 8-18 wt% of a filler and the balance of the diatomite-beet armyworm nuclear polyhedrosis virus composition.
The wettable powder is a solid powdery pesticide formulation, is convenient to store and transport, can be mixed with water according to needs when in use, and is very convenient, but the diatomite-beet armyworm nucleopolyhedrosis virus composition in the application has hydrophobicity, is difficult to dissolve and disperse in water when preparing a liquid medicine, has poor adhesion of effective components on the surface of a plant after spraying, is easy to be influenced by weather such as wind, rain and the like, and can solve various problems when in use of the pesticide by matching of various auxiliary agents because beet armyworm nucleopolyhedrosis virus is sensitive to ultraviolet rays and the effective components need to be attached to the back of a leaf surface when spraying and can also increase the pesticide effect on hidden insects.
In some preferred embodiments, the dispersant is selected from the group consisting of HMK-D1003, HMK-D1005, HMK-806, Morwet D425, Morwet D450, Morwet D500, Morwet D110, Morwet EFW, and Morwet IP; further preferably, the dispersant is Morwet D425.
In some preferred embodiments, the dispersant further comprises Synfac 8120.
In some preferred embodiments, the weight ratio of Morwet D425 to Synfac 8120 is (4-6): 1; further preferably, the weight ratio of the Morwet D425 to the Synfac 8120 is 5: 1.
the Morwet system products in this application are produced by Acksonobel, and the Synfac series products are produced by Milliken.
The Morwet series products are alkyl naphthalene sulfonate surfactants, namely anionic surfactants, it is ionized in water solution, so that it has high solubility and can improve the dispersivity of the system obviously, the water quality used for diluting the pesticide composition is not uniform, some have the problem of high hardness, and the sulfonate group existing in Morwet is complexed with metal ions in water, so that the effect of the dispersant is poor, however, the inventor unexpectedly discovers that, when the Morwet and Synfac series are used in combination, the adaptability of the medicament under different water quality conditions can be ensured, this is because the Synfac 8120 can just compensate the dispersion effect of Morwet lost due to complexation in hard water environment, but if the ratio of the two is not properly controlled, micelles are formed in the soft water and a uniform liquid medicine cannot be obtained as well, and the inventors have found in a great deal of practice, when the weight ratio of Morwet D425 to Synfac 8120 is (4-6): 1, under the condition of soft water, the two surfactants cause weak acting force between micelles due to steric hindrance of molecules, so that the micelles are difficult to exist stably, and the application range of the pesticide composition in different water qualities is expanded.
In some preferred embodiments, the wetting agent is selected from nekal and/or Morwet EFW; further preferably, the wetting agent is Morwet EFW, produced by meliken.
In some preferred embodiments, the filler is selected from a mixture of one or more of kaolin, talc, calcium carbonate, bentonite, attapulgite, silica; further preferably, the filler is white carbon black.
The white carbon black in the present application may be commercially available, for example, wacker germany.
In some preferred embodiments, the weight ratio of the dispersing agent, the wetting agent and the filler is (1-2): 1: (2-3); further preferably, the weight ratio of the dispersing agent, the wetting agent and the filler is 1.5: 1: 2.5.
the inventor carefully researches and discovers that when the weight ratio of the dispersing agent to the wetting agent to the filler is (1-2): 1: (2-3), the prepared wettable powder has small granularity, no caking phenomenon, high suspension rate of the diluted liquid medicine, short wetting time and improved drug effect, and the reason is that after part of liquid raw materials are adsorbed and mixed by using a filler with a higher oil absorption value, the caking phenomenon caused by the influence of the liquid raw materials on the kieselguhr is reduced, so that the kieselguhr can be smoothly dispersed in the liquid medicine to play the insecticidal action of a special structure of the kieselguhr, but when the using amount of the filler is too much, the relative content of the kieselguhr is reduced, and the insecticidal effect of the wettable powder is reduced; the wetting agent is helpful for spreading the liquid medicine on the surface of the plant, is also an auxiliary agent capable of changing the surface performance, and the dosage ratio of the dispersing agent and the wetting agent needs to be strictly controlled, so that on one hand, the liquid medicine is a uniform, stable and dispersed system when being stored, on the other hand, the liquid medicine can be rapidly spread on the surfaces of leaves and the like when being applied, and the dosage of the filler is determined by the dosage of the surfactant, so that the selection and dosage of the wetting agent, the dispersing agent and the surfactant are closely related.
In some preferred embodiments, the potentiator is a monosaccharide or polysaccharide.
In some preferred embodiments, the monosaccharide is selected from a mixture of one or more of glucose, mannose, fructose, galactose; further preferably, the monosaccharide is glucose.
In some preferred embodiments, the polysaccharide is selected from a mixture of one or more of starch, sucrose, cellulose, gum arabic, xanthan gum; further preferably, the polysaccharide is starch.
The inventor finds that monosaccharide or polysaccharide is added into wettable powder as a synergist to improve the adhesion of the medicament on the surface of a plant, and the reason is that the molecular structure of saccharide substances contains a large number of active groups, so that a bridge can be built between the medicament and the surface of the plant, the bonding force between the medicament and the plant is increased, and some saccharide substances can provide certain viscosity for the liquid medicament, so that the stability of the liquid medicament is ensured, the adhesion time of the medicament on the surface of the plant can be further prolonged, and the action time of the medicament is further prolonged.
The fourth aspect of the invention provides a preparation method of the diatomite-spodoptera exigua nucleopolyhedrosis virus wettable powder, which comprises the following steps: pulverizing and mixing the above raw materials except diatomaceous earth, adding diatomaceous earth, shaking, and stirring.
Examples
The technical solution of the present invention is described in detail by the following examples, but the scope of the present invention is not limited to the examples. Unless otherwise specified, the starting materials in the present invention are all commercially available.
Example 1
Co-toxicity coefficient (CTC) screening: measuring the combined toxicity of the compounded diatomite and beet armyworm nucleopolyhedrosis virus to prodenia litura; the diatomaceous earth has a saw-tooth structure (Yirui stone, France, Celite 610).
Referring to the method in NY/T1154.14-2008 standard, the leaf-dipping dish method is adopted. Beat the cabbage leaf into the leaf dish with the hole puncher of diameter 18mm, dip the leaf dish in the liquid of testing medicine for 10 seconds after, put into the culture dish (diameter 90mm) that has filled with the filter paper that moisturizes, 6 leaf dishes are put into to every dish, wait to dry the back naturally, select again and starve 4h twill noctuid 3 age larva to the culture dish in, every dish is not less than 10, 4 times are repeated, the processing finishes, covers the ware lid, arranges the indoor culture of observation in.
And 7d, detecting the death condition of the test insects, and recording, wherein the death judgment standard of the test insects is that no response is calculated when the tweezers lightly contact the test insects.
The co-toxicity coefficient (CTC) of each mixed combination is determined by a Sun & Johnson (1960) co-toxicity coefficient method (CTC) according to a raw test standard method NY/T1154.7-2006. Generally, a co-toxicity coefficient greater than 120 indicates a synergistic effect, less than 80 indicates an antagonistic effect, and between 80 and 120 indicates an additive effect. The co-toxicity coefficient is calculated as follows
The Theoretical Toxicity Index (TTI) of the mixture is the toxicity index of agent a × the percentage (%) of agent a + the toxicity index of agent B × the percentage (%) of agent B.
The results of the co-toxicity coefficient (CTC) and the Theoretical Toxicity Index (TTI) of the combination are shown in Table 1.
TABLE 1 toxicity test results of Spodoptera litura by blending of diatomaceous earth and Spodoptera exigua nuclear polyhedrosis virus
Example 2
Co-toxicity coefficient (CTC) screening: measuring the combined toxicity of the compounded diatomite and beet armyworm nuclear polyhedrosis virus on tea geometrid; the diatomaceous earth has a saw-tooth structure (Yirui stone, France, Celite 610).
Referring to the method in NY/T1154.14-2008 standard, the leaf soaking method is adopted. Selecting 3 fresh tea tree sprouts, soaking in the test liquid medicine for 30 seconds, taking out, naturally drying, wrapping the bases of the sprouts with wet absorbent cotton to preserve moisture, sealing absorbent cotton balls with a preservative film, and placing in a can bottle, wherein the concentration is repeated for 4 times. Then inoculating 2-year-old nymphs of tea geometrid with consistent size and liveness and health into a brush pen, wherein 15 +/-2 heads of each pot are sealed by white gauze. After the treatment, the sample is placed in an observation chamber.
And 7d, detecting the death condition of the test insects, and recording, wherein the death judgment standard of the test insects is that no response is calculated when the tweezers lightly contact the test insects.
The co-toxicity coefficient (CTC) of each mixed combination is determined by a Sun & Johnson (1960) co-toxicity coefficient method (CTC) according to a raw test standard method NY/T1154.7-2006. Generally, a co-toxicity coefficient greater than 120 indicates a synergistic effect, less than 80 indicates an antagonistic effect, and between 80 and 120 indicates an additive effect. The co-toxicity coefficient is calculated as follows
The Theoretical Toxicity Index (TTI) of the mixture is the toxicity index of agent a × the percentage (%) of agent a + the toxicity index of agent B × the percentage (%) of agent B.
The results of the co-toxicity coefficient (CTC) and the Theoretical Toxicity Index (TTI) of the combination are shown in Table 2.
TABLE 2 toxicity test results of blending of diatomaceous earth and beet armyworm nuclear polyhedrosis virus to tea geometrid
Example 3
Embodiment 3 provides a diatomaceous earth-spodoptera exigua nucleopolyhedrosis virus wettable powder, which is prepared from the following raw materials: 6 wt% of dispersing agent, 4 wt% of wetting agent, 5 wt% of synergist, 10 wt% of filler and the balance of diatomite-beet armyworm nuclear polyhedrosis virus composition.
The diatomite-beet armyworm nuclear polyhedrosis virus composition comprises raw medicines of diatomite and beet armyworm nuclear polyhedrosis virus; the asparagus caterpillar nuclear polyhedrosis virus raw drug is 2000 hundred million PIB/gram of asparagus caterpillar nuclear polyhedrosis virus raw powder, and is purchased from Jiyuan Baiyun industry Co., Ltd, Henan province; the diatomite has a sawtooth structure and is produced by France Yirui stone company, and the mark is Celite 610; the weight ratio of the diatomite to the spodoptera exigua nuclear polyhedrosis virus raw pesticide is 400: 1.
the dispersing agent is a mixture of Morwet D425 and Synfac 8120, and the weight ratio is 5: 1; the wetting agent is Morwet EFW; the synergist is glucose; the filler is white carbon black.
The embodiment also provides a preparation method of the diatomite-beet armyworm nucleopolyhedrosis virus wettable powder, which comprises the following steps: pulverizing and mixing the above raw materials except diatomaceous earth, adding diatomaceous earth, shaking, and stirring.
Example 4
Embodiment 4 provides a diatomaceous earth-spodoptera exigua nucleopolyhedrosis virus wettable powder, which is prepared from the following raw materials: 6 wt% of dispersing agent, 4 wt% of wetting agent, 5 wt% of synergist, 10 wt% of filler and the balance of diatomite-beet armyworm nuclear polyhedrosis virus composition.
The diatomite-beet armyworm nuclear polyhedrosis virus composition comprises raw medicines of diatomite and beet armyworm nuclear polyhedrosis virus; the asparagus caterpillar nuclear polyhedrosis virus raw drug is 2000 hundred million PIB/gram of asparagus caterpillar nuclear polyhedrosis virus raw powder, and is purchased from Jiyuan Baiyun industry Co., Ltd, Henan province; the diatomite has a sawtooth structure and is produced by France Yirui stone company, and the mark is Celite 610; the weight ratio of the diatomite to the spodoptera exigua nuclear polyhedrosis virus raw pesticide is 400: 1.
the dispersant is Morwet D425; the wetting agent is Morwet EFW; the synergist is glucose; the filler is white carbon black.
This example also provides a method for preparing wettable powder of diatomaceous earth-beet armyworm nucleopolyhedrosis virus as described above, which is similar to example 3.
Example 5
Embodiment 5 provides a diatomaceous earth-beet armyworm nuclear polyhedrosis virus wettable powder, which is prepared from the following raw materials: 6 wt% of dispersing agent, 4 wt% of wetting agent, 5 wt% of synergist, 10 wt% of filler and the balance of diatomite-beet armyworm nuclear polyhedrosis virus composition.
The diatomite-beet armyworm nuclear polyhedrosis virus composition comprises raw medicines of diatomite and beet armyworm nuclear polyhedrosis virus; the asparagus caterpillar nuclear polyhedrosis virus raw drug is 2000 hundred million PIB/gram of asparagus caterpillar nuclear polyhedrosis virus raw powder, and is purchased from Jiyuan Baiyun industry Co., Ltd, Henan province; the diatomite has a sawtooth structure and is produced by France Yirui stone company, and the mark is Celite 610; the weight ratio of the diatomite to the spodoptera exigua nuclear polyhedrosis virus raw pesticide is 400: 1.
the dispersing agent is a mixture of Morwet D425 and Synfac 8120, and the weight ratio is 1: 1; the wetting agent is Morwet EFW; the synergist is glucose; the filler is white carbon black.
This example also provides a method for preparing wettable powder of diatomaceous earth-beet armyworm nucleopolyhedrosis virus as described above, which is similar to example 3.
Example 6
Embodiment 6 provides a diatomaceous earth-spodoptera exigua nucleopolyhedrosis virus wettable powder, which is prepared from the following raw materials: 6 wt% of dispersing agent, 4 wt% of wetting agent, 5 wt% of synergist, 10 wt% of filler and the balance of diatomite-beet armyworm nuclear polyhedrosis virus composition.
The diatomite-beet armyworm nuclear polyhedrosis virus composition comprises raw medicines of diatomite and beet armyworm nuclear polyhedrosis virus; the asparagus caterpillar nuclear polyhedrosis virus raw drug is 2000 hundred million PIB/gram of asparagus caterpillar nuclear polyhedrosis virus raw powder, and is purchased from Jiyuan Baiyun industry Co., Ltd, Henan province; the diatomite has a sawtooth structure and is produced by France Yirui stone company, and the mark is Celite 610; the weight ratio of the diatomite to the spodoptera exigua nuclear polyhedrosis virus raw pesticide is 400: 1.
the dispersing agent is a mixture of Morwet D425 and Synfac 8120, and the weight ratio is 5: 1; the wetting agent is Morwet EFW; the synergist is starch; the filler is white carbon black.
This example also provides a method for preparing wettable powder of diatomaceous earth-beet armyworm nucleopolyhedrosis virus as described above, which is similar to example 3.
Example 7
Embodiment 7 provides a diatomaceous earth-spodoptera exigua nucleopolyhedrosis virus wettable powder, which is prepared from the following raw materials: 6 wt% of dispersing agent, 4 wt% of wetting agent, 5 wt% of synergist and the balance of diatomite-beet armyworm nuclear polyhedrosis virus composition.
The diatomite-beet armyworm nuclear polyhedrosis virus composition comprises raw medicines of diatomite and beet armyworm nuclear polyhedrosis virus; the asparagus caterpillar nuclear polyhedrosis virus raw drug is 2000 hundred million PIB/gram of asparagus caterpillar nuclear polyhedrosis virus raw powder, and is purchased from Jiyuan Baiyun industry Co., Ltd, Henan province; the diatomite has a sawtooth structure and is produced by France Yirui stone company, and the mark is Celite 610; the weight ratio of the diatomite to the spodoptera exigua nuclear polyhedrosis virus raw pesticide is 400: 1.
the dispersing agent is a mixture of Morwet D425 and Synfac 8120, and the weight ratio is 5: 1; the wetting agent is Morwet EFW; the synergist is glucose.
This example also provides a method for preparing wettable powder of diatomaceous earth-beet armyworm nucleopolyhedrosis virus as described above, which is similar to example 3.
Example 8
Embodiment 8 provides a diatomaceous earth-spodoptera exigua nucleopolyhedrosis virus wettable powder, which is prepared from the following raw materials: 6 wt% of dispersing agent, 4 wt% of wetting agent, 10 wt% of filler and the balance of diatomite-beet armyworm nuclear polyhedrosis virus composition.
The diatomite-beet armyworm nuclear polyhedrosis virus composition comprises raw medicines of diatomite and beet armyworm nuclear polyhedrosis virus; the asparagus caterpillar nuclear polyhedrosis virus raw drug is 2000 hundred million PIB/gram of asparagus caterpillar nuclear polyhedrosis virus raw powder, and is purchased from Jiyuan Baiyun industry Co., Ltd, Henan province; the diatomite has a sawtooth structure and is produced by France Yirui stone company, and the mark is Celite 610; the weight ratio of the diatomite to the spodoptera exigua nuclear polyhedrosis virus raw pesticide is 400: 1.
the dispersing agent is a mixture of Morwet D425 and Synfac 8120, and the weight ratio is 5: 1; the wetting agent is Morwet EFW; the filler is white carbon black.
This example also provides a method for preparing wettable powder of diatomaceous earth-beet armyworm nucleopolyhedrosis virus as described above, which is similar to example 3.
Example 9
Embodiment 9 provides a diatomaceous earth-spodoptera exigua nucleopolyhedrosis virus wettable powder, which is prepared from the following raw materials: 8 wt% of dispersing agent, 3 wt% of wetting agent, 5 wt% of synergist, 11 wt% of filler and the balance of diatomite-beet armyworm nuclear polyhedrosis virus composition.
The diatomite-beet armyworm nuclear polyhedrosis virus composition comprises raw medicines of diatomite and beet armyworm nuclear polyhedrosis virus; the asparagus caterpillar nuclear polyhedrosis virus raw drug is 2000 hundred million PIB/gram of asparagus caterpillar nuclear polyhedrosis virus raw powder, and is purchased from Jiyuan Baiyun industry Co., Ltd, Henan province; the diatomite has a sawtooth structure and is produced by France Yirui stone company, and the mark is Celite 610; the weight ratio of the diatomite to the spodoptera exigua nuclear polyhedrosis virus raw pesticide is 400: 1.
the dispersing agent is a mixture of Morwet D425 and Synfac 8120, and the weight ratio is 5: 1; the wetting agent is Morwet EFW; the synergist is glucose; the filler is white carbon black.
This example also provides a method for preparing wettable powder of diatomaceous earth-beet armyworm nucleopolyhedrosis virus as described above, which is similar to example 3.
Example 10
Embodiment 10 provides a diatomaceous earth-spodoptera exigua nucleopolyhedrosis virus wettable powder, which is prepared from the following raw materials: 7 wt% of dispersing agent, 7 wt% of wetting agent, 5 wt% of synergist, 14 wt% of filler and the balance of diatomite-beet armyworm nuclear polyhedrosis virus composition.
The diatomite-beet armyworm nuclear polyhedrosis virus composition comprises raw medicines of diatomite and beet armyworm nuclear polyhedrosis virus; the asparagus caterpillar nuclear polyhedrosis virus raw drug is 2000 hundred million PIB/gram of asparagus caterpillar nuclear polyhedrosis virus raw powder, and is purchased from Jiyuan Baiyun industry Co., Ltd, Henan province; the diatomite has a sawtooth structure and is produced by France Yirui stone company, and the mark is Celite 610; the weight ratio of the diatomite to the spodoptera exigua nuclear polyhedrosis virus raw pesticide is 400: 1.
the dispersing agent is a mixture of Morwet D425 and Synfac 8120, and the weight ratio is 5: 1; the wetting agent is MorwetEFW; the synergist is glucose; the filler is white carbon black.
This example also provides a method for preparing wettable powder of diatomaceous earth-beet armyworm nucleopolyhedrosis virus as described above, which is similar to example 3.
Evaluation of Performance
The performance of examples 3 to 10 was evaluated, and the evaluation contents include suspension percentage, wetting time, fineness, surface tension, and contact angle.
1. Measurement of suspension percentage: measured according to the method of GB/T14825-2006
The method comprises the following steps: the sample to be tested is prepared into a suspension of appropriate concentration with standard hard water. And (3) standing in a measuring cylinder for a certain time under a specified condition, measuring the mass fraction of the effective components in the suspension at one tenth of the bottom, and calculating the suspension rate. The results are shown in Table 3.
2. Determination of the wetting time: measured according to the method of GB/T5451-2001
The method comprises the following steps: a quantity of the wettable powder is poured from a specified height into a beaker containing a quantity of standard hard water and the time to complete wetting is measured. The results are shown in Table 3.
3. Measurement of fineness: measured according to the "wet sieving method" of GB/T16150-1995 method
The method comprises the following steps: placing the weighed sample in a beaker for wetting and diluting, pouring the sample into a wetted test sieve, directly washing the test sieve by using a gentle tap water flow, placing the test sieve in a basin containing water for continuously washing, transferring the residue in the sieve to the beaker, drying the residue, weighing and calculating the fineness. The test sieve is a 325-mesh test sieve, the fineness is more than or equal to 98 percent, and the result is shown in table 3.
4. Determination of surface tension
The method comprises the following steps: the prepared wettable powder is prepared into 500 times and 1000 times of diluent, and the surface tension of the diluent is measured by a surface tension meter. The results are shown in Table 3.
5. Determination of contact Angle
The method comprises the following steps: the prepared wettable powder is prepared into 500 times and 1000 times of diluent, and the contact angle of the diluent is measured by a contact angle measuring instrument. The results are shown in Table 3.
TABLE 3 results of the measurements of the indexes
Through the embodiments 1 and 2, the compound use of the diatomite with the sawteeth and the spodoptera exigua nuclear polyhedrosis virus has excellent synergistic effect on the control of lepidoptera pests, the balance of suspension rate and wetting time can be realized under the action of specific additives and proportion through the primarily screened wettable powder formula, the liquid medicine prepared by the liquid medicine is ensured to have a uniform and stable system, the binding force between effective components and the surface of a plant can be improved, the acting time is shortened, the drug effect is prolonged, and the preparation method is suitable for being widely popularized and used in production.
The foregoing examples are merely illustrative and serve to explain some of the features of the method of the present invention. The appended claims are intended to claim as broad a scope as is contemplated, and the examples presented herein are merely illustrative of selected implementations in accordance with all possible combinations of examples. Accordingly, it is applicants' intention that the appended claims are not to be limited by the choice of examples illustrating features of the invention. Also, where numerical ranges are used in the claims, subranges therein are included, and variations in these ranges are also to be construed as possible being covered by the appended claims.
Claims (10)
1. A diatomite-spodoptera exigua nuclear polyhedrosis virus composition, which is characterized by comprising raw materials of diatomite and spodoptera exigua nuclear polyhedrosis virus; the diatomite has a sawtooth structure.
2. The diatomaceous earth-spodoptera exigua nuclear polyhedrosis virus composition of claim 1, wherein the bulk spodoptera exigua nuclear polyhedrosis virus is 2000 billion PIB per gram spodoptera exigua nuclear polyhedrosis virus raw powder.
3. The diatomaceous earth-spodoptera exigua nuclear polyhedrosis virus composition of claim 1 or claim 2, wherein the weight ratio of the diatomaceous earth to spodoptera exigua nuclear polyhedrosis virus bulk drug is (1: 40) - (400: 1).
4. Use of the diatomaceous earth-spodoptera exigua nucleopolyhedrosis virus composition of any one of claims 1 to 3, for lepidopteran insect control.
5. The use of the diatomaceous earth-spodoptera exigua nuclear polyhedrosis virus composition of claim 4, wherein the lepidoptera insect is selected from one or more of the group consisting of prodenia litura, spodoptera exigua, tea geometrid, spodoptera frugiperda.
6. A diatomite-beet armyworm nuclear polyhedrosis virus wettable powder which is characterized in that raw materials for preparation comprise the diatomite-beet armyworm nuclear polyhedrosis virus composition as claimed in any one of claims 1-3, a dispersing agent, a wetting agent, a synergist and a filler.
7. The wettable powder of claim 6, wherein the dispersant is selected from the group consisting of HMK-D1003, HMK-D1005, HMK-806, Morwet D425, Morwet D450, Morwet D500, Morwet D110, Morwet EFW, and Morwet IP.
8. The wettable powder of diatomaceous earth-beet armyworm nuclear polyhedrosis virus of claim 6, wherein the synergist is a monosaccharide or polysaccharide.
9. The wettable powder of the diatomite and the spodoptera exigua nuclear polyhedrosis virus of claim 6, wherein the weight ratio of the dispersing agent to the wetting agent to the filler is (1-2): 1: (2-3).
10. The preparation method of the diatomite-spodoptera exigua nucleopolyhedrosis virus wettable powder as defined in any one of claims 6 to 9, which comprises the following steps: pulverizing and mixing the above raw materials except diatomaceous earth, adding diatomaceous earth, shaking, and stirring.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112335686A (en) * | 2020-11-12 | 2021-02-09 | 江苏省农业科学院 | Method for preventing and controlling Spodoptera frugiperda by utilizing Spodoptera litura nuclear polyhedrosis virus at different recommended levels in various regions |
| CN112931533A (en) * | 2021-02-02 | 2021-06-11 | 中国林业科学研究院森林生态环境与保护研究所 | Forestry insect nucleopolyhedrosis virus wettable powder and preparation method thereof |
-
2020
- 2020-02-19 CN CN202010100929.7A patent/CN111213677A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112335686A (en) * | 2020-11-12 | 2021-02-09 | 江苏省农业科学院 | Method for preventing and controlling Spodoptera frugiperda by utilizing Spodoptera litura nuclear polyhedrosis virus at different recommended levels in various regions |
| CN112931533A (en) * | 2021-02-02 | 2021-06-11 | 中国林业科学研究院森林生态环境与保护研究所 | Forestry insect nucleopolyhedrosis virus wettable powder and preparation method thereof |
| CN112931533B (en) * | 2021-02-02 | 2022-03-11 | 中国林业科学研究院森林生态环境与保护研究所 | Forestry insect nucleopolyhedrosis virus wettable powder and preparation method thereof |
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