CN114982760A - Leek bradysia odoriphaga larva phagostimulant and determination method of bait trapping rate - Google Patents
Leek bradysia odoriphaga larva phagostimulant and determination method of bait trapping rate Download PDFInfo
- Publication number
- CN114982760A CN114982760A CN202210528008.XA CN202210528008A CN114982760A CN 114982760 A CN114982760 A CN 114982760A CN 202210528008 A CN202210528008 A CN 202210528008A CN 114982760 A CN114982760 A CN 114982760A
- Authority
- CN
- China
- Prior art keywords
- methyl
- trisulfide
- bait
- composition
- phagostimulant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
- A01N41/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom not containing sulfur-to-oxygen bonds, e.g. polysulfides
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/002—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits
- A01N25/006—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits insecticidal
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Insects & Arthropods (AREA)
- Food Science & Technology (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
Abstract
The invention provides a leek bradysia odoriphaga larva phagostimulant and a method for determining bait trapping rate, wherein the composition is a combination of at least two of dimethyl trithioether, methyl propyl trithioether, methyl allyl trithioether or 2-methyl-2-pentenal. The bradysia odoriphaga larva phagostimulant composition disclosed by the invention can directly attract a considerable amount of bradysia odoriphaga larvae from tuber of Chinese chive, obviously weakens the concealment of bradysia odoriphaga, increases the exposure degree, lays a foundation for the effective action of medicament control measures, and can achieve the purposes of reducing quantity, increasing efficiency and reducing cost.
Description
Technical Field
The invention belongs to the field of agricultural pest control, and particularly relates to a bradysia odoriphaga larvas phagostimulant and a method for determining bait trapping rate.
Background
Bradysia odoriphaga, also called leek maggot, is a phytophagous insect, has a rich recipe, and is commonly found in leek, shallot, garlic and other crops, and is often found in other plants such as liliaceae, cucurbitaceae, cruciferae, umbelliferae, chrysanthemumaceae and chenopodiaceae as hosts. The bradysia odoriphaga is only harmful in the larval stage, and when the bradysia odoriphaga is seriously harmful, the larva can move at the cut of the bradysia odoriphaga when the bradysia odoriphaga is clustered to eat the underground rhizome part of the plant. The affected plants are characterized by wilting and yellow leaves, thin and weak seedlings, death of the plants in severe cases, seedling shortage and ridge breaking in fields.
In agricultural production, because of the concealment of the larvae of bradysia odoriphaga, the prevention and control of the larvae often requires the application of a large dose of a high-toxicity chemical pesticide. Therefore, research on the trapping technology of the bradysia odoriphaga larvae weakens the hidden characteristic of the bradysia odoriphaga larvae in harmfulness, improves the control efficiency of the pesticide, and is a demand for modern agricultural development, particularly for organic leek production.
Disclosure of Invention
In view of the above, the present invention provides a bradysia odoriphaga larva phagostimulant and a method for determining bait trapping rate, which aims to overcome the defects in the prior art.
In order to achieve the purpose, the technical scheme of the invention is realized as follows:
a food attractant composition for bradysia odoriphaga larvae is a combination of at least two of dimethyl trisulfide, methyl propyl trisulfide, methyl allyl trisulfide or 2-methyl-2-pentenal.
Preferably, the composition is a combination of methallyl trisulfide and at least one of dimethyl trisulfide, methylpropyl trisulfide or 2-methyl-2-pentenal.
Preferably, the composition is a combination of 2-methyl-2-pentenal and methallyl trisulfide, and the mass ratio of the 2-methyl-2-pentenal to the methallyl trisulfide is 1-8: 1 to 6; the composition is a combination of dimethyl trisulfide, methyl propyl trisulfide and methyl allyl trisulfide, and the mass ratio of the dimethyl trisulfide to the methyl propyl trisulfide to the methyl allyl trisulfide is 0.1-4: 0.1-4: 0.1 to 6; the composition is a combination of dimethyl trisulfide, 2-methyl-2-pentenal and methyl allyl trisulfide, and the mass ratio of the dimethyl trisulfide to the 2-methyl-2-pentenal to the methyl allyl trisulfide is (0.1-4): 0.1-8: 0.1 to 6; the composition is a combination of methyl propyl trithioether, 2-methyl-2-pentenal and methyl allyl trithioether, and the mass ratio of the methyl propyl trithioether, the 2-methyl-2-pentenal and the methyl allyl trithioether is (0.1-4): 0.1-8: 0.1-6.
Preferably, the composition is dimethyl trisulfide, methyl propyl trisulfide, methyl allyl trisulfide and 2-methyl-2-pentenal; the mass ratio of the dimethyl trisulfide, the methyl propyl trisulfide, the methyl allyl trisulfide to the 2-methyl-2-pentenal is 0-10: 0-10: 0.1-12: 0 to 20; preferably, the mass ratio of the dimethyl trisulfide, the methyl propyl trisulfide and the methyl allyl trisulfide to the 2-methyl-2-pentenal is 0-4: 0-4: 0.1-6: 0 to 8; more preferably, the mass ratio of the dimethyl trisulfide, the methyl propyl trisulfide and the methyl allyl trisulfide to the 2-methyl-2-pentenal is 0-4: 0-4: 0.1-6: 0.2 to 8; more preferably, the mass ratio of the dimethyl trisulfide, the methyl propyl trisulfide and the methyl allyl trisulfide to the 2-methyl-2-pentenal is 0-4: 0.1-4: 0.1-6: 0-8.
More preferably, the mass ratio of dimethyl trisulfide, methyl propyl trisulfide, methyl allyl trisulfide to 2-methyl-2-pentenal is 1: 1: 1: 2.
a bradysia odoriphaga larva phagostimulant, which comprises the bradysia odoriphaga larva phagostimulant composition of any one of claims 1-4, wherein the composition accounts for 0.00001-1% of the total mass of the phagostimulant; preferably, the composition accounts for 0.0001-0.1% of the total mass of the phagostimulant; preferably, the composition accounts for 0.0001-0.08% of the total mass of the phagostimulant.
A bradysia odoriphaga larva bait, which comprises 100-300 parts of barley powder, 120-280 parts of corn powder, 1-5 parts of lentinan and 300-450 parts of the phagostimulant in claim 5.
A method for determining bait trapping rate of bradysia odoriphaga larvae comprises the following steps:
(1) 1 central platform is placed in the center of the vessel, a plurality of bait platforms are uniformly placed on the outer side of the central platform, and the distances between the centers of the bait platforms and the center of the central platform are the same;
(2) putting Chinese chives on a central platform, inoculating larvae on the Chinese chives, covering soil, and compacting;
(3) placing equal mass of said bait according to claim 6 on each bait station, covering with soil, and compacting;
(4) and counting the number of larvae on each bait platform after culturing, and calculating the trapping rate.
Further, the structure of the central platform in the step (1) is the same as that of the bait platform; the bait platform comprises a stand and a net rack, and the net rack is fixed on the stand; the number of the bait stations is more than 2.
Further, the water content of the soil in the step (2) is the same as that of the soil in the step (3); the water content of the soil in the step (2) is 16-23%.
Further, the humidity of the culture step in the step (4) is 10-80%, the temperature is 10-35 ℃, and the time is 1-72 hours.
Compared with the prior art, the invention has the following advantages:
the bradysia odoriphaga larva phagostimulant composition can directly attract a considerable amount of bradysia odoriphaga larvae from the roots and stems of Chinese chives, obviously weakens the concealment of bradysia odoriphaga, increases the exposure degree, lays a foundation for the effective action of medicament control measures, and can achieve the purposes of reducing the quantity, increasing the efficiency and reducing the cost.
The method for determining the bait trapping rate of the bradysia odoriphaga larvae comprises the steps of determining by using a bait table, wherein a latticed net rack reduces the resistance of the larvae close to the bait, and the arrangement of the table defines the bait trapping range, so that the bait trapping amount can be conveniently counted; the method is carried out in a soil environment with the humidity range of 16-23% and the depth range of 1-10cm, the moving capability of larvae in the soil is fully guaranteed, light interference is eliminated, and the accurate expression of the trapping effect of the phagostimulant is ensured.
Drawings
Fig. 1 is a schematic view of a bait station according to an embodiment of the invention;
FIG. 2 is a schematic diagram showing the distribution of the center station and the bait station in the vessel according to the first test method of example 3;
FIG. 3 is a schematic view of a culture dish according to the second test method in example 3 of the present invention.
Description of reference numerals:
1. a bait station; 2. a stand; 3. a net frame; 4. a center table.
Detailed Description
Unless defined otherwise, technical terms used in the following examples have the same meanings as commonly understood by one of ordinary skill in the art to which the present invention belongs. The test reagents used in the following examples, unless otherwise specified, are all conventional biochemical reagents; the experimental methods are conventional methods unless otherwise specified.
The present invention will be described in detail with reference to examples.
Example 1 determination of the response rate and selection coefficient of bradysia odoriphaga larvae phagostimulant compositions
1. Assembling the Y-shaped insect olfactometer. An atmosphere sampling instrument QC-1B, an air purification bottle (filled with granular activated carbon), an air humidifier (300mL conical bottle filled with deionized water) and a Y-shaped glass tube (the length of three arms is 50mm, and the inner diameter is 6mm) are connected in sequence by using a silica gel hose (the inner diameter is 6mm and the outer diameter is 8 mm). After the air tightness of the assembled Y-shaped insect sniffer is checked, the air flow of the air sampling instrument is set to be 100 mL/min, and the environmental temperature is adjusted to be 25 ℃.
2. Selecting 3-year-old chive bradysia odoriphaga larvae before test, and starving for 12 h. Before the inoculation, the thin hairbrush is soaked in distilled water, then 5 test insects are picked, placed at about 1/2 position in the air outlet of the Y-shaped glass tube, the larvae are scattered, and the tail of the larvae is slightly poked by the tip of the brush head to enter a motion state. The test insects crawl against the wind and select the odor source, timing is started when the bodies of the larvae 2/3 enter the corresponding Y-shaped glass tube arms of the odor source, and the larvae stay for enough 2min to be considered as selection.
3. The relative positions of the control bottle and the odor bottle are immediately exchanged after one group of 5 larvae is determined, and the Y-shaped glass tube is replaced. The amount of composition used in each test was 20 μ L, and was dropped onto a 350mm × 200mm rectangular filter paper using a pipette, changing the filter paper and re-dosing each time one set was completed or more than 10 min. The test uses fresh leeks as a control, the fresh leeks are ground into homogenate and smeared on filter paper with the same specification, and the best fresh weight of 0.4g of leeks is determined through pre-test. 20000-time dilution preparation is carried out on the bradysia odoriphaga larva feeding attractant composition with different proportions by using 8% acetone solution, 10mL of each gradient dilution of each composition is prepared, and the preparation method comprises the following steps: first, the pipette is used to aspirate the drug according to the compounding ratio in table 1, 1: 1, mixing and proportioning, and respectively sucking 0.05 mu L of each medicament; 3:6, mixing and proportioning, and respectively sucking 0.15 mu L and 0.3 mu L of each medicament; 3: 11, each medicine is sucked by 0.15 mu L and 0.55 mu L respectively. The absorbed medicament needs to be dissolved in 0.8mL of acetone, shaken up, then distilled water is added into the liquid medicine to be constant volume of 10mL, and fully shaken up to prepare the phagostimulant. Sealing for standby. In the test of attractant effect for each composition, dilution gradients for each attractant were measured in 4 groups for a total of 20 subjects. And replacing a new smell bottle after each testing of one phagostimulant. The composition ratios and results are shown in table 1.
And (3) calculating the selective reaction rate and the selective coefficient according to the measurement result, wherein the formula is as follows:
note: the selection coefficient is greater than 0, which indicates that the test insects have tropism to the taste source substances, the numerical value is larger, which indicates that the tropism is stronger, and the maximum value is 1; and if the selection coefficient is less than zero, the selection coefficient is negative, which indicates that the test insects have negative tropism to the taste source.
TABLE 1 selection coefficient of multi-mix Allium tuberosum bradysia odoriphaga larva phagostimulant composition
As can be seen from Table 1, the compounding ratio of the compositions B to C to the compositions H to K is 1: 1/1: 1: 1/1: 1: 1: 1 or 3: 6/3: 3: 6/3: 3:3: at 6 (except for the 3:3:6:3 mixing ratio of composition K), the selection coefficients are positive numbers, which indicate that at this mixing ratio, bradysia odoriphaga larvae have positive tropism for it, and the ratio of 1: 1/1: 1: 1/1: 1: 1: the selection coefficients under the mixing ratio of 1 are all more than 3: 6/3: 3: 6/3: 3:3: selection factor at 6 compounding ratio.
The selection coefficients for composition a and compositions D-G were negative at all three mixing ratios, indicating that chive bradysia odoriphaga larvae were negatively taxied at this mixing ratio. However, in 3: 6/3: 3: 6/3: 3:3: under the mixing proportion of 6, the selection coefficient is larger than the numerical values of other mixing proportion examples, and under the mixing proportion, the proportion of ether and aldehyde is about 1: 2. according to the above analysis, the ratio of the four agents can be further determined as the mixing ratio of the trithioether substance and the aldehyde substance is set as 1: 2.
example 2 determination of the reaction Rate and selection coefficient of the phagostimulant for bradysia odoriphaga larvae
1. Selecting 3-year-old chive bradysia odoriphaga larvae before test, and starving for 12 h. Before the inoculation, the thin hairbrush is soaked in distilled water, then 5 test insects are picked, placed at about 1/2 position in the air outlet of the Y-shaped glass tube, the larvae are scattered, and the tail of the larvae is slightly poked by the tip of the brush head to enter a motion state. The test insects crawl against the wind and select the odor source, timing is started when the bodies of the larvae 2/3 enter the corresponding Y-shaped glass tube arms of the odor source, and the larvae stay for enough 2min to be considered as selection.
2. The screening test uses 5 larvae as a group, and the relative positions of the control bottle and the odor bottle are immediately exchanged after the group is determined, and the Y-shaped glass tube is replaced. Each test composition was applied in an amount of 20. mu.L, dropped on a 350mm X200 mm rectangular filter paper using a pipette, and the filter paper was changed and the drug was re-applied each time one set or more than 10min was completed. The test takes fresh leeks as a control, the fresh leeks are ground into homogenate and smeared on filter paper with the same specification, and the best fresh weight of 0.4g of leeks is determined through pre-test. The compositions are diluted by 8% acetone solution to obtain the phagostimulant, the phagostimulant is subjected to the luring effect test from high dilution times to low dilution times, and each composition is subjected to 4 groups of dilution gradients respectively, and the total number is 20. Each time one composition was tested, the odor bottle was replaced with a new one. The specific dilution and results are shown in tables 2-4. The calculation formula is the same as that of example 1.
TABLE 2 selection coefficients of bradysia odoriphaga larva phagostimulants with different dilution gradients
As can be seen from Table 2, the distribution rules of the selection coefficients of the three compositions are different in the dilution gradient interval of 1250-. In the gradient dilution range of the two substance compositions, the selection coefficient of 20000-160000-fold liquid is positive, reaches the maximum value at 20000-fold liquid, and the selection coefficient is 0.1 at 160000-fold liquid and approaches to 0; the selection coefficients of the 10000 times of liquid in the low dilution range 1250 and the selection coefficient is negative, and the selection coefficient is-1 when the lowest dilution multiple is 1250 times. Within the gradient dilution range of the three-substance composition, the selection coefficient of 2500-; and the selection coefficient of only 1250 times of liquid in the low dilution region is negative, and the selection coefficient is-0.35. Within the range of gradient dilution of the four compositions of matter, the selection coefficient of only 20000 times the liquid is positive. The distribution characteristics of the selection coefficients of the 2, 3 and 4 substance compositions in the gradient dilution range of 1250-.
Example 3 Allium tuberosum Levl et Schw larval bait disposition and trapping ability assay
Preparing a bait: the food bait is prepared from barley, corn, lentinan and a phagostimulant according to the mass ratio of 150:150:3:350, the food bait prepared from barley, corn, lentinan and water according to the same proportion is used as a control group, and the trapping rate of the food bait is measured. The compositions of the bait are shown in the table below.
TABLE 3 compositions in baits
The first test method comprises the following steps: soil bait burying method
And (3) picking sufficient amount of bradysia odoriphaga larvae of 3-year-old leek, placing the bradysia odoriphaga larvae into a culture dish paved with wet filter paper, and starving for 24 hours for later use. Weighing 2kg of dried soil, adding distilled water, respectively adjusting the water content of the soil to 16%, 20%, 23% and 26%, and uniformly stirring for later use. Taking enough fresh leek stems, cutting into 3mm stem segments for later use.
A center table is placed at the center of a 15cm glass culture dish, and 1.2g of leek stem segments are placed on the center table. 80 starvation-treated larvae of bradysia odoriphaga of 3 years old chives were inoculated on the central platform chives. 1.2g of bait is weighed and agglomerated into a ball shape, the ball is placed in the center of the net surface of a plastic bait platform, and a contrast bait is placed on the bait platform at the symmetrical position. And finally covering the soil, slightly pressing the surface of the soil to keep the thickness of the soil consistent, wherein the thickness of the soil is 2.5cm, covering the culture dish, and placing the culture dish in a constant-temperature incubator with the humidity of 60% and the temperature of 25 ℃. And (3) testing 5 food baits, wherein each food bait is processed in 4 soil humidity for three times, after 24 hours, taking out the plastic breathable buttons with soil, counting the number of larvae on each plastic breathable button, and calculating the trapping rate of the food baits under each soil humidity, wherein the trapping rate calculation formula is as follows.
And (2) a second test method: filter paper bare trapping method
A12 cm petri dish was prepared and laid on wet filter paper. Food bait 1.2g was weighed, pelletized, and placed in the wet filter paper mark position, as shown in fig. 3, with the treatment and control groups placed symmetrically. 1.2g of stem segments (3 mm in length) of leek are placed at the center of a center circle (d is 3cm), 20 larvae of 3-instar bradysia odoriphaga subjected to starvation treatment for 24 hours are inoculated, a culture dish cover is covered, and the culture dish is placed in a lightless constant-temperature incubator at the temperature of 25 ℃ and the humidity of 60%. Each culture dish is treated for three times, the quantity of the larvae staying in each shadow part is counted after 12 hours (the larvae on the pressed line are counted in the shadow area), and the trapping rate is calculated. The trapping rate of 5 kinds of food baits is determined by the same test method I, and the calculation formula of the trapping rate is as follows.
TABLE 4 luring rate of bradysia odoriphaga larva baits
As can be seen from table 4, in the soil bait burying test, the trapping rate of each food bait to bradysia odoriphaga larvae was the highest at a soil water content of 20%, the trapping effect was the lowest at a soil water content of 16%, and the trapping rates were all 0. Each food bait contained the composition "dimethyltrisulfide: methyl propyl trisulfide: the food bait trapping effect of the methyl allyl trisulfide is the best, the trapping rate is the highest when the water content of soil is 20%, and the trapping rate reaches 42.50%. The treatment trapping rate of the soil with the water content of 23 percent is the second highest and reaches 30.15 percent. The treatment trapping effect is poor when the water content of the soil is 26 percent, and the maximum value is only 15.40 percent; the water content of the soil is 16%, the trapping effect is the worst, and the trapping rate of the food bait is 0. It can be seen that when the soil moisture content is 23%, the bradysia odoriphaga larvae have the strongest migration ability in the soil and the food bait has the best odor diffusion effect in the soil. When the water content of the soil is low, the migration capacity of the bradysia odoriphaga is greatly hindered, so that the trapping rate of the food bait is 0. When the soil moisture content is higher, the trapping rate of the food bait is reduced, which is associated with the influence of the diffusion ability of the odor of the food bait in the high-moisture soil. Therefore, the soil moisture content of 20% is selected as the moisture content standard of the test soil. In a filter paper naked trapping test, the trapping rate of each food bait is similar to the trapping rate of a soil bait burying method in the size difference trend, but the overall level is higher, particularly the treatment with lower trapping effect in the soil bait burying test is more obvious, if the components are methyl propyl trisulfide: the composition and components of the methyl allyl trisulfide are dimethyl trisulfide: methyl propyl trisulfide: 2-methyl-2-pentenal: methallyl trisulfide ".
Compared with two test methods, the trapping rate of each bait under the condition of 20% of soil water content is obviously lower than that of exposed trapping on the filter paper, and the trapping effect of each food bait is weakened. On the one hand, in the test of determining the trapping rate by the soil bait burying method, soil is used as a habitat of bradysia odoriphaga larvae, so that the diffusion effect of odor in the soil is hindered, and the movement capability of the bradysia odoriphaga larvae in the soil is obviously influenced by the water content of the soil. And the bait is exposed on the wet filter paper for trapping, so that the environmental resistance under natural conditions is eliminated, and the trapping capacity of the bait is higher. On the other hand, the soil bait burying method requires no other operation during the test. In the filter paper exposure trapping method, shading treatment is required to eliminate light interference. The filter paper has limited water retention capacity, and the larva has moving capacity by periodically supplementing water, so that the operation is more complicated. Therefore, the soil bait burying method can better simulate the natural growth environment of the bradysia odoriphaga and the determined trapping rate is more authentic. In addition, in the statistical method, the naked trapping of the filter paper requires statistics of the larvae on the filter paper in a larger range around the bait at a certain time, so that the larvae in a wandering state are easily counted, which may increase experimental errors. The soil bait burying method only counts the insect quantity on the bait platform, the volume of the bait platform is smaller and is similar to the volume of the food bait, and the representativeness of the data is more accurate than that of the filter paper naked trapping method.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (10)
1. A bradysia odoriphaga larva feeding promoting composition is characterized in that: the composition is a combination of at least two of dimethyltrisulfide, methylpropyltrisulfide, methallyltrisulfide or 2-methyl-2-pentenal.
2. The bradysia odoriphaga larva phagostimulant composition of claim 1, wherein: the composition is the combination of methyl allyl trisulfide and at least one of dimethyl trisulfide, methyl propyl trisulfide or 2-methyl-2-pentenal.
3. The bradysia odoriphaga larva phagostimulant composition of claim 2, wherein: the composition is a combination of 2-methyl-2-pentenal and methyl allyl trisulfide, and the mass ratio of the 2-methyl-2-pentenal to the methyl allyl trisulfide is 1-8: 1 to 6; the composition is a combination of dimethyl trisulfide, methyl propyl trisulfide and methyl allyl trisulfide, and the mass ratio of the dimethyl trisulfide to the methyl propyl trisulfide to the methyl allyl trisulfide is 0.1-4: 0.1-4: 0.1 to 6; the composition is a combination of dimethyl trisulfide, 2-methyl-2-pentenal and methyl allyl trisulfide, and the mass ratio of the dimethyl trisulfide to the 2-methyl-2-pentenal to the methyl allyl trisulfide is (0.1-4): 0.1-8: 0.1 to 6; the composition is a combination of methyl propyl trithioether, 2-methyl-2-pentenal and methyl allyl trithioether, and the mass ratio of the methyl propyl trithioether, the 2-methyl-2-pentenal and the methyl allyl trithioether is (0.1-4): 0.1-8: 0.1-6.
4. The bradysia odoriphaga larva phagostimulant composition of claim 2, wherein: the composition is the combination of dimethyl trisulfide, methyl propyl trisulfide, methyl allyl trisulfide and 2-methyl-2-pentenal; the mass ratio of the dimethyl trisulfide, the methyl propyl trisulfide, the methyl allyl trisulfide to the 2-methyl-2-pentenal is 0-10: 0-10: 0.1-12: 0 to 20; preferably, the mass ratio of the dimethyl trisulfide, the methyl propyl trisulfide, the methyl allyl trisulfide to the 2-methyl-2-pentenal is 0-4: 0-4: 0.1-6: 0 to 8; more preferably, the mass ratio of the dimethyl trisulfide, the methyl propyl trisulfide and the methyl allyl trisulfide to the 2-methyl-2-pentenal is 0-4: 0-4: 0.1-6: 0.2 to 8; more preferably, the mass ratio of the dimethyl trisulfide, the methyl propyl trisulfide and the methyl allyl trisulfide to the 2-methyl-2-pentenal is 0-4: 0.1-4: 0.1-6: 0-8.
5. A bradysia odoriphaga larva phagostimulant is characterized in that: the phagostimulant comprises the bradysia odoriphaga larva phagostimulant composition of any one of claims 1 to 4, wherein the composition accounts for 0.00001 to 1 percent of the total mass of the phagostimulant; preferably, the composition accounts for 0.0001-0.1% of the total mass of the phagostimulant; preferably, the composition accounts for 0.0001-0.08% of the total mass of the phagostimulant.
6. A leek bradysia odoriphaga larva bait is characterized in that: the bait comprises 300 parts of barley flour 100, 280 parts of corn flour 120, lentinan 1-5 parts and 450 parts of the phagostimulant in claim 5.
7. A determination method of bait trapping rate of bradysia odoriphaga larvae is characterized by comprising the following steps: the method comprises the following steps:
(1) 1 central platform is placed in the center of the vessel, a plurality of bait platforms are uniformly placed on the outer side of the central platform, and the distances between the centers of the bait platforms and the center of the central platform are the same;
(2) placing Chinese chives on the central platform, inoculating larvae on the Chinese chives, covering soil, and compacting;
(3) placing the bait of claim 6 in equal mass on each bait station, covering with soil, and compacting;
(4) and counting the number of larvae on each bait platform after culturing, and calculating the trapping rate.
8. The method for determining the bait trapping rate of bradysia odoriphaga larvae according to claim 7, wherein the method comprises the following steps: the structure of the central platform in the step (1) is the same as that of the bait platform; the bait platform comprises a stand and a net rack, and the net rack is fixed on the stand; the number of the bait stations is more than 2.
9. The method for determining the bait trapping rate of bradysia odoriphaga larvae according to claim 7, wherein the bait trapping rate is determined by using the following steps: the water content of the soil in the step (2) is the same as that of the soil in the step (3); the water content of the soil in the step (2) is 16-23%.
10. The method for determining the bait trapping rate of bradysia odoriphaga larvae according to claim 7, wherein the method comprises the following steps: the humidity of the culture step in the step (4) is 10-80%, the temperature is 10-35 ℃, and the time is 1-72 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210528008.XA CN114982760B (en) | 2022-05-16 | 2022-05-16 | Method for measuring bait trapping rate of slow-eye muscadine larvae phagostimulant for Chinese chives |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210528008.XA CN114982760B (en) | 2022-05-16 | 2022-05-16 | Method for measuring bait trapping rate of slow-eye muscadine larvae phagostimulant for Chinese chives |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114982760A true CN114982760A (en) | 2022-09-02 |
| CN114982760B CN114982760B (en) | 2023-07-28 |
Family
ID=83026715
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210528008.XA Active CN114982760B (en) | 2022-05-16 | 2022-05-16 | Method for measuring bait trapping rate of slow-eye muscadine larvae phagostimulant for Chinese chives |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114982760B (en) |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6201026B1 (en) * | 1999-03-09 | 2001-03-13 | The Regents Of The University Of California | Volatile aldehydes as pest control agents |
| CN101341934A (en) * | 2007-07-13 | 2009-01-14 | 东莞市盛唐化工有限公司 | Citrus fruit fly food attractant |
| CN102100230A (en) * | 2010-12-29 | 2011-06-22 | 华南农业大学 | New use of 2-methyl-2-pentenal serving as bactericide |
| CN103399126A (en) * | 2013-08-02 | 2013-11-20 | 山东农业大学 | Indoor bioassay method of bradysia odoriphaga larvae |
| CN103766342A (en) * | 2014-02-28 | 2014-05-07 | 广东省农业科学院果树研究所 | Insecticide stock solution and application thereof |
| CN104170814A (en) * | 2014-07-24 | 2014-12-03 | 青岛农业大学 | Monitoring and trap-killing technology for bradysia odoriphaga |
| CN107258789A (en) * | 2017-08-17 | 2017-10-20 | 浙江省农业科学院 | Attractant of Bradysia odoriphaga and preparation method and application |
| CN111296441A (en) * | 2020-03-16 | 2020-06-19 | 北京嘉景生物科技有限责任公司 | Core attractant for attracting locust and core attractant and application thereof |
| WO2020222296A1 (en) * | 2019-04-27 | 2020-11-05 | 株式会社アクト・フォ | Beverage, food composition, health food composition, oral composition and taste improver |
| CN111903676A (en) * | 2020-06-27 | 2020-11-10 | 内蒙古大学 | Attractant for pollinating insects, method for improving plant maturing rate and application thereof |
| CN113498795A (en) * | 2021-08-17 | 2021-10-15 | 北京中农绿安有机农业科技有限公司 | Preparation method of pure natural plant acaricide |
-
2022
- 2022-05-16 CN CN202210528008.XA patent/CN114982760B/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6201026B1 (en) * | 1999-03-09 | 2001-03-13 | The Regents Of The University Of California | Volatile aldehydes as pest control agents |
| CN101341934A (en) * | 2007-07-13 | 2009-01-14 | 东莞市盛唐化工有限公司 | Citrus fruit fly food attractant |
| CN102100230A (en) * | 2010-12-29 | 2011-06-22 | 华南农业大学 | New use of 2-methyl-2-pentenal serving as bactericide |
| CN103399126A (en) * | 2013-08-02 | 2013-11-20 | 山东农业大学 | Indoor bioassay method of bradysia odoriphaga larvae |
| CN103766342A (en) * | 2014-02-28 | 2014-05-07 | 广东省农业科学院果树研究所 | Insecticide stock solution and application thereof |
| CN104170814A (en) * | 2014-07-24 | 2014-12-03 | 青岛农业大学 | Monitoring and trap-killing technology for bradysia odoriphaga |
| CN107258789A (en) * | 2017-08-17 | 2017-10-20 | 浙江省农业科学院 | Attractant of Bradysia odoriphaga and preparation method and application |
| WO2020222296A1 (en) * | 2019-04-27 | 2020-11-05 | 株式会社アクト・フォ | Beverage, food composition, health food composition, oral composition and taste improver |
| CN111296441A (en) * | 2020-03-16 | 2020-06-19 | 北京嘉景生物科技有限责任公司 | Core attractant for attracting locust and core attractant and application thereof |
| CN111903676A (en) * | 2020-06-27 | 2020-11-10 | 内蒙古大学 | Attractant for pollinating insects, method for improving plant maturing rate and application thereof |
| CN113498795A (en) * | 2021-08-17 | 2021-10-15 | 北京中农绿安有机农业科技有限公司 | Preparation method of pure natural plant acaricide |
Non-Patent Citations (1)
| Title |
|---|
| 董民;张顶武;杜相革;: "有机桃园天敌与蚜虫的互作关系及生态调控措施", 植物保护学报, no. 04, pages 327 - 332 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114982760B (en) | 2023-07-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| SõGAWA et al. | Mechanisms of brown planthopper resistance in Mudgo variety of rice (Hemiptera: Delphacidae) | |
| Brødsgaard | Insecticide resistance in European and African strains of western flower thrips (Thysanoptera: Thripidae) tested in a new residue-on-glass test | |
| Konvicka et al. | Habitat of pre-hibernating larvae of the endangered butterfly Euphydryas aurinia (Lepidoptera: Nymphalidae): What can be learned from vegetation composition and architecture? | |
| Broadhead | The Psocid fauna of larch trees in Northern England--an ecological study of mixed species populations exploiting a common resource | |
| CN111165485A (en) | Diatomite-beauveria bassiana pesticide composition and application thereof | |
| Zegada-Lizarazu et al. | Water competition of intercropped pearl millet with cowpea under drought and soil compaction stresses | |
| Lv et al. | The seed semipermeable layer and its relation to seed quality assessment in four grass species | |
| Juvik et al. | Influence of trichome exudates from species of Lycopersicon on oviposition behavior of Heliothis zea (Boddie) | |
| CN103918611A (en) | Simple wheat aphid species assay method | |
| CN114982760A (en) | Leek bradysia odoriphaga larva phagostimulant and determination method of bait trapping rate | |
| CN101571532A (en) | Novel method for measuring biological activity of biological pesticides | |
| Metcalf | Methods for the study of pest Diabrotica | |
| Rabinowitz et al. | Abundance and neighborhood structure for sparse and common grasses in a Missouri prairie | |
| Michalska et al. | Oviposition by the vagrant eriophyoid mite Aculops allotrichus on leaves of black locust tree, Robinia pseudoacacia | |
| CN111202057A (en) | Insecticidal pesticide composition containing diatomite with special structure and application thereof | |
| CN111183986A (en) | Pesticide containing diatomite and spinetoram and preparation method thereof | |
| CN116746579B (en) | Spodoptera frugiperda oviposition repellent pheromone and application thereof | |
| CN113785828B (en) | Plant source attractant for astragalus mongholicus and apis cerana and application of plant source attractant | |
| CN110583645A (en) | Harmonia axyridis plant source attractant and application thereof | |
| Furlan et al. | Observations on natural mortality factors in wireworm populations and evaluation of management options | |
| CN112098638B (en) | Method for determining toxicity of pesticide to wallbee larvae | |
| CN111213673A (en) | Insecticidal composition containing diatomite, and medicament and preparation method thereof | |
| CN115462375B (en) | Application of amino acid in promoting pesticide absorption of crops | |
| Mathwig | Relationships between bruchid beetles (Amblycerus robiniae) and honey locust trees (Gleditsia triacanthos) | |
| CN111689808B (en) | Inducer for promoting quality improvement and yield increase of plants and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |