CN114949064A - Preparation method of traditional Chinese medicine microecological preparation for treating enteritis and diarrhea of laying hens - Google Patents

Preparation method of traditional Chinese medicine microecological preparation for treating enteritis and diarrhea of laying hens Download PDF

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CN114949064A
CN114949064A CN202011492512.6A CN202011492512A CN114949064A CN 114949064 A CN114949064 A CN 114949064A CN 202011492512 A CN202011492512 A CN 202011492512A CN 114949064 A CN114949064 A CN 114949064A
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traditional chinese
chinese medicine
diarrhea
preparation
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CN114949064B (en
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史洪涛
王坤丽
李长滨
张立恒
乔宏兴
边传周
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Henan University of Animal Husbandry and Economy
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Henan University of Animal Husbandry and Economy
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    • A61K36/185—Magnoliopsida (dicotyledons)
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Abstract

The invention belongs to the technical field of traditional Chinese veterinary medicines, and particularly relates to a preparation method of a traditional Chinese medicine microecological preparation for treating enteritis and diarrhea of laying hens. The method comprises the steps of soaking a traditional Chinese medicine decoction piece composition in water, boiling, concentrating to obtain a water extract, inoculating lactobacillus plantarum into the water extract, and performing anaerobic fermentation; the traditional Chinese medicine decoction piece composition is composed of the following traditional Chinese medicine decoction pieces in parts by weight: 280-320 parts of Chinese pulsatilla root, 280-320 parts of purslane, 130-170 parts of giant knotweed rhizome, 180-220 parts of amur corktree bark, 90-110 parts of rhubarb, 130-170 parts of dark plum fruit, 90-110 parts of myrobalan, 180-220 parts of costus root, 550-650 parts of swordlike atractylodes rhizome, 90-110 parts of lightyellow sophora root, 90-110 parts of pomegranate bark, 90-110 parts of honeysuckle stem, 90-110 parts of common andrographis herb and 45-55 parts of liquorice. The traditional Chinese medicine microecological preparation prepared by the invention can enhance intestinal digestion and absorption functions and immunity of laying hens, promotes health recovery of organisms, and has obvious curative effects on treating enteritis and diarrhea of the laying hens.

Description

Preparation method of traditional Chinese medicine microecological preparation for treating enteritis and diarrhea of laying hens
Technical Field
The invention belongs to the technical field of traditional Chinese veterinary medicines, and particularly relates to a preparation method of a traditional Chinese medicine microecological preparation for treating enteritis and diarrhea of laying hens.
Background
The intensive production mode enables the laying hens to be in a stress sensitive state, reduces the immune function, the intestinal function and the like of organisms, improves the sensitivity to pathogenic microorganisms, and causes the problems of enteritis and diarrhea of the laying hens. In addition, mycotoxin contamination in the feed also easily causes enteritis, diarrhea and reproductive system diseases, and causes the morbidity and mortality of the feed to be increased.
The previous method for solving the problems of enteritis and diarrhea of the laying hens is mainly to add antibiotics in feed and drinking water, but the long-term large-dosage addition of the antibiotics can cause a plurality of problems: such as the generation of drug-resistant strains and the overproof drug residues in eggs, and can cause serious environmental pollution and threaten the health of people. The use of antibiotics in a large amount in the brooding period can cause the problems of intestinal flora imbalance, intestinal digestion and absorption function obstruction, serious bacterial drug resistance and the like.
Disclosure of Invention
The invention aims to provide a preparation method of a traditional Chinese medicine microecological preparation for treating enteritis and diarrhea of laying hens, which can effectively treat the problems of enteritis and diarrhea of the laying hens.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a preparation method of a traditional Chinese medicine microecological preparation for treating enteritis and diarrhea of laying hens comprises the following steps: soaking the Chinese medicinal tablet composition in water, boiling, concentrating to obtain water extractive solution, inoculating Lactobacillus plantarum, and performing anaerobic fermentation; the traditional Chinese medicine decoction piece composition is composed of the following traditional Chinese medicine decoction pieces in parts by weight: 280-320 parts of Chinese pulsatilla root, 280-320 parts of purslane, 130-170 parts of giant knotweed rhizome, 180-220 parts of amur corktree bark, 90-110 parts of rhubarb, 130-170 parts of dark plum fruit, 90-110 parts of myrobalan, 180-220 parts of costus root, 550-650 parts of swordlike atractylodes rhizome, 90-110 parts of lightyellow sophora root, 90-110 parts of pomegranate bark, 90-110 parts of honeysuckle stem, 90-110 parts of common andrographis herb and 45-55 parts of liquorice.
According to the preparation method of the traditional Chinese medicine microecological preparation, the traditional Chinese medicine decoction piece composition can enhance intestinal digestion and absorption functions and immunity of laying hens after the raw materials are combined, promotes the health of organisms, and has obvious curative effects on treating enteritis and diarrhea of the laying hens; after lactobacillus plantarum fermentation, the absorption and curative effect of the Chinese herbal medicines are further improved, and the curative effect on enteritis and diarrhea of the laying hens is enhanced.
The traditional Chinese medicine microecological preparation prepared by the method utilizes the lactobacillus plantarum to carry out anaerobic fermentation on the traditional Chinese medicine, thereby enhancing the absorption and utilization of the medicinal components; in the metabolic process of the lactobacillus plantarum, a large amount of organic acids such as acetic acid, propionic acid and the like and digestive enzymes can reduce the pH value of the gastrointestinal tract, and are beneficial to activating and improving the activity of pepsin, and the growth and reproduction of pathogenic bacteria can be effectively inhibited in an acidic environment; the lactein in the metabolite has the effect of inhibiting or killing escherichia coli, salmonella and the like; the lactobacillus plantarum and the metabolites thereof cooperate with the Chinese herbal medicine components, so that the intestinal microenvironment can be effectively realized, the development of intestinal immune tissues can be stimulated, the intestinal immune function can be improved, and the favorable treatment of enteritis and diarrhea of the laying hens can be further realized.
By adopting the traditional Chinese medicine microecological preparation, the problems of slow action effect and low utilization rate of the traditional Chinese herbal medicine are solved; secondly, the application of antibiotics can be reduced, so that the problems of bacterial drug resistance, drug residue, mycotoxin pollution of feed and the like are solved; finally, the intestinal digestion and absorption function and immunity of the laying hens are enhanced, and the body is promoted to be recovered.
In the traditional Chinese medicine decoction piece composition, the characteristics of the raw materials are as follows:
the Chinese pulsatilla root: cold in nature, bitter in taste; entering large intestine and stomach meridians; has effects of clearing away heat and toxic materials, cooling blood, and relieving dysentery; it is mainly used for treating diarrhea due to damp-heat, and dysentery with bloody stool due to toxic heat.
Purslane: cold in nature, sour in taste; it enters liver and large intestine meridians; has effects in clearing away heat and toxic materials, cooling blood, and relieving dysentery; it is mainly used for treating dysentery due to toxic heat, sore, yellow swelling, and toxic materials.
Giant knotweed rhizome: slightly cold in nature and sweet in taste; entering liver and kidney meridians; has effects of clearing away heat and toxic materials, promoting diuresis, eliminating jaundice, promoting blood circulation, and dredging collaterals; it can be used for treating damp-heat jaundice, blood stasis and pain, carbuncle, swelling, skin sore, and rheumatalgia.
Phellodendron bark: cold in nature, bitter in taste; entering kidney and bladder meridians; has effects in clearing away damp-heat, purging pathogenic fire, and relieving deficiency heat; it is used to treat dysentery due to damp-heat pathogen, jaundice, night sweat due to yin deficiency, pyocutaneous disease, and toxic swelling.
Rhubarb: cold in nature, bitter in taste; it is entered into spleen, stomach, large intestine, liver and pericardium; has effects in removing food stagnation, purging pathogenic fire, cooling blood, promoting blood circulation, and dispelling blood stasis; it can be used for treating constipation due to heat accumulation, sore, swelling, blood stasis, burn, and scald.
Dark plum: mild in nature, sour and astringent in taste; it enters liver, spleen, lung and large intestine meridians; has effects in astringing lung, astringing intestine, promoting salivation, quenching thirst, expelling intestinal parasites, and relieving dysentery; it is mainly used for treating chronic cough, chronic diarrhea and chronic dysentery due to lung deficiency.
Myrobalan fruit: warm in nature, bitter, sour, astringent in taste; entering lung and large intestine meridians; has effects in relieving diarrhea with astringents, astringing lung, and relieving cough; it is used to treat chronic diarrhea and dysentery, hematochezia, rectocele, cough and asthma due to lung deficiency.
Costustoot: warm in nature, pungent and slightly bitter in flavor; the spleen, stomach, large intestine and gallbladder meridians are entered; has effects in promoting qi circulation, relieving pain, regulating stomach function, and relieving diarrhea; it is mainly used for treating qi stagnation of spleen and stomach, indigestion, damp-heat diarrhea and dysentery.
Rhizoma atractylodis: warm in nature, pungent and bitter in taste; spleen and stomach meridians entered; has the effects of eliminating dampness, strengthening spleen, dispelling pathogenic wind and eliminating dampness. It can be used for treating damp obstruction of spleen and stomach, arthralgia due to wind-cold-dampness, and nyctalopia.
Flavescent sophora root: cold in nature, bitter in taste; it enters heart, liver, stomach, large intestine and bladder channels; has the functions of clearing heat, eliminating dampness, dispelling wind, killing parasites and promoting urination; it can be used for treating jaundice due to damp-heat pathogen, dysentery, scabies, and tinea.
Pomegranate rind: warm in nature, sour and astringent in taste; entering the large intestine meridian; has the effects of astringing, stopping diarrhea and killing parasites; it is indicated for chronic diarrhea, dysentery and worm accumulation.
Honeysuckle stem: cold in nature and sweet in taste; entering lung and stomach meridians; has the functions of clearing away heat and toxic material, dispelling wind and dredging collaterals; it can be used for treating epidemic febrile disease, fever, dysentery, skin ulcer, furunculosis, and arthralgia due to wind-damp-heat.
Andrographis paniculata: cold in nature, bitter in taste; it enters lung, stomach, large intestine and small intestine meridians; has effects of clearing away heat and toxic materials, eliminating dampness, and relieving diarrhea; it is mainly indicated for cough and dyspnea due to lung heat, diarrhea due to yellow intestine, and dysentery.
Licorice root: mild in nature and sweet in taste; entering twelve meridians; has the effects of invigorating spleen and replenishing qi, clearing away heat and toxic materials, moistening lung for arresting cough, and relieving drug property. Can be used for treating weakness of spleen and stomach, cough, sore, carbuncle, sore throat, and poisoning.
Preferably, the traditional Chinese medicine decoction piece composition consists of the following traditional Chinese medicine decoction pieces in parts by weight: 300 parts of Chinese pulsatilla root, 300 parts of purslane, 150 parts of giant knotweed, 200 parts of amur corktree bark, 100 parts of rhubarb, 150 parts of dark plum fruit, 100 parts of myrobalan, 200 parts of costus root, 600 parts of swordlike atractylodes rhizome, 100 parts of lightyellow sophora root, 100 parts of pomegranate rind, 100 parts of honeysuckle stem, 100 parts of common andrographis herb and 50 parts of liquorice.
Preferably, the weight of the water extract is 1.5-2 times of the weight of the traditional Chinese medicine decoction piece composition, the inoculation amount of lactobacillus plantarum is 1-10% of the weight of the water extract, and the viable count of lactobacillus plantarum is (1.0-20.0) × 10 8 CFU/mL. More preferably, the inoculation amount of lactobacillus plantarum is 7% of the weight of the water extract, and the viable count of lactobacillus plantarum is 1.0 × 10 8 CFU/mL. The inoculation amount is weight volume percentage, and represents the inoculation milliliter number of the liquid lactobacillus plantarum microbial inoculum corresponding to each 100 grams of water extract. In the invention, the general characteristics of the lactobacillus plantarum are applied, and the current commercially available varieties can meet the requirements.
Preferably, the temperature of the anaerobic fermentation is 28-37 ℃, and the time is 48-72 hours. Further preferably, anaerobic fermentation is carried out at 37 ℃ for 60 h.
In order to improve the utilization rate of the raw materials and the extraction efficiency of the medicinal ingredients, the water adding amount is preferably 5-10 times of that of the traditional Chinese medicine decoction piece composition, the soaking time is 12-24 hours, and the boiling time is 2-4 hours. More preferably, the extract is boiled in water and extracted 2 to 3 times, and the extracts are combined and then concentrated. After boiling, the mixture is preferably autoclaved at 115-121 ℃ for 30-60 min.
The usage of the traditional Chinese medicine microecological preparation prepared by the method comprises the following steps: every 1000mL of the solution is added with 1000-1200 jin of water for 3-5 days.
Detailed Description
The following examples are provided to further illustrate the practice of the invention.
The specific embodiment of the preparation method of the traditional Chinese medicine microecological preparation for treating enteritis and diarrhea of laying hens of the invention
Example 1
The preparation method of the traditional Chinese medicine microecological preparation for treating enteritis and diarrhea of laying hens comprises the following steps:
preparing a traditional Chinese medicine decoction piece composition according to the following parts by weight: 280 parts of Chinese pulsatilla root, 280 parts of purslane, 130 parts of giant knotweed, 180 parts of phellodendron, 90 parts of rhubarb, 130 parts of dark plum, 90 parts of myrobalan, 180 parts of costus root, 550 parts of rhizoma atractylodis, 90 parts of lightyellow sophora root, 90 parts of pomegranate rind, 90 parts of honeysuckle stem, 90 parts of common andrographis herb and 45 parts of liquorice.
Adding 5 times of water into the decoction pieces, soaking for 24 hr, boiling for 2 hr, extracting for 3 times, concentrating to 1.5 times of the weight of the Chinese medicinal materials, autoclaving at 115 deg.C for 60min, cooling to 30 deg.C, inoculating Lactobacillus plantarum with an inoculum size of 10% of the extract weight, the viable count of Lactobacillus plantarum strain is 1.0 × 10 8 CFU/mL, anaerobic fermenting at 28 deg.C for 60 hr, terminating fermentation, and packaging.
The usage of the traditional Chinese medicine microecological preparation for treating enteritis and diarrhea of laying hens in the embodiment is as follows: every 1000mL of the solution is added with 1200 jin of water for 3-5 days.
Example 2
The preparation method of the traditional Chinese medicine microecological preparation for treating enteritis and diarrhea of laying hens comprises the following steps:
preparing a traditional Chinese medicine decoction piece composition according to the following parts by weight: 320 parts of Chinese pulsatilla root, 320 parts of purslane, 170 parts of giant knotweed, 220 parts of phellodendron, 110 parts of rhubarb, 170 parts of dark plum, 110 parts of myrobalan, 220 parts of costus root, 650 parts of rhizoma atractylodis, 110 parts of kuh-seng, 110 parts of pomegranate rind, 110 parts of honeysuckle stem, 110 parts of common andrographis herb and 55 parts of liquorice.
Adding 10 times of water into the Chinese medicinal decoction pieces composition, soaking for 12 hr, boiling for 3 hr, extracting for 2 times, concentrating to 2 times of the weight of Chinese medicinal materials, autoclaving at 121 deg.C for 60min, cooling to 35 deg.C, inoculating Lactobacillus plantarum and Lactobacillus plantarum stem in an amount of 1% of the weight of the extractive solutionThe viable count of the strain is 2.0 × 10 9 CFU/mL, anaerobic fermenting at 35 deg.C for 48 hr, terminating fermentation, and packaging.
The usage of the traditional Chinese medicine microecological preparation for treating enteritis and diarrhea of laying hens in the embodiment is as follows: every 1000mL of the solution is added with 1000 jin of water for 3-5 days.
Example 3
The preparation method of the traditional Chinese medicine microecological preparation for treating enteritis and diarrhea of laying hens comprises the following steps:
preparing a traditional Chinese medicine decoction piece composition according to the following parts by weight: 300 parts of Chinese pulsatilla root, 300 parts of purslane, 150 parts of giant knotweed, 200 parts of phellodendron, 100 parts of rhubarb, 150 parts of dark plum, 100 parts of myrobalan, 200 parts of costus root, 600 parts of rhizoma atractylodis, 100 parts of sophora flavescens, 100 parts of pomegranate bark, 100 parts of honeysuckle stem, 100 parts of common andrographis herb and 50 parts of liquorice.
Adding 8 times of water into the Chinese medicinal decoction pieces composition, soaking for 18 hr, boiling for 3 hr, extracting for 2 times, concentrating to 1.5 times of the weight of Chinese medicinal materials, autoclaving at 121 deg.C for 40min, cooling to 40 deg.C, inoculating Lactobacillus plantarum with an inoculum size of 7% of the extract weight, wherein the viable count of Lactobacillus plantarum strain is 1.0 × 10 9 CFU/mL, anaerobic fermenting at 37 deg.C for 60 hr, terminating fermentation, and packaging.
The usage of the traditional Chinese medicine microecological preparation for treating enteritis and diarrhea of laying hens in the embodiment is as follows: every 1000mL of the solution is mixed with 1200 jin of water for 3-5 days.
Example 4
The preparation method of the traditional Chinese medicine microecological preparation for treating enteritis and diarrhea of laying hens comprises the following steps:
preparing a traditional Chinese medicine decoction piece composition according to the following parts by weight: 290 parts of Chinese pulsatilla root, 290 parts of purslane, 160 parts of giant knotweed, 190 parts of phellodendron, 100 parts of rhubarb, 160 parts of dark plum, 110 parts of myrobalan, 210 parts of costus root, 620 parts of rhizoma atractylodis, 100 parts of sophora flavescens, 110 parts of pomegranate bark, 100 parts of honeysuckle stem, 100 parts of common andrographis herb and 50 parts of liquorice.
Adding 6 times of water into the Chinese medicinal decoction pieces composition, soaking for 16 hr, boiling for 2 hr, extracting for 3 times, concentrating to 2 times of the weight of Chinese medicinal materials, autoclaving at 115 deg.C for 50min, cooling to 30 deg.C, and inoculating according to 3% of the weight of extractive solutionThe viable count of the lactobacillus plantarum strain is 5.0 multiplied by 10 8 CFU/mL, anaerobic fermenting at 28 deg.C for 72h, terminating fermentation, and packaging.
The usage of the traditional Chinese medicine microecological preparation for treating enteritis and diarrhea of laying hens in the embodiment is as follows: every 1000mL of the solution is added with 1000 jin of water for 3-5 days.
Example 5
The preparation method of the traditional Chinese medicine microecological preparation for treating enteritis and diarrhea of laying hens comprises the following steps:
preparing a traditional Chinese medicine decoction piece composition according to the following parts by weight: 310 parts of Chinese pulsatilla root, 310 parts of purslane, 140 parts of giant knotweed, 210 parts of phellodendron, 100 parts of rhubarb, 140 parts of dark plum, 90 parts of myrobalan, 190 parts of costus root, 550 parts of rhizoma atractylodis, 90 parts of sophora flavescens, 90 parts of pomegranate bark, 110 parts of honeysuckle stem, 110 parts of common andrographis herb and 55 parts of liquorice.
Adding 8 times of water into the Chinese medicinal decoction pieces composition, soaking for 20 hr, boiling for 4 hr, extracting for 2 times, concentrating to 2 times of the weight of Chinese medicinal materials, autoclaving at 121 deg.C for 30min, cooling to 40 deg.C, inoculating Lactobacillus plantarum with viable count of 1.5 × 10 according to 10% of the weight of extractive solution 9 CFU/mL, anaerobic fermenting at 37 deg.C for 48 hr, terminating fermentation, and packaging.
The usage of the traditional Chinese medicine microecological preparation for treating enteritis and diarrhea of laying hens in the embodiment is as follows: every 1000mL of the solution is added with 1000 jin of water for 3-5 days.
Second, comparative example
The traditional Chinese medicine preparation for treating enteritis and diarrhea of laying hens in the comparative example is prepared by adding 8 times of water into the traditional Chinese medicine decoction piece composition in the example 3, soaking for 18h, boiling for 3h, extracting for 2 times, concentrating to be 1.5 times of the weight of the traditional Chinese medicines, autoclaving at 121 ℃ for 40min, cooling to 40 ℃, and subpackaging.
Third, test example
Test example 1
The experimental example examines the treatment effect of the traditional Chinese medicine microecologics in different embodiments on enteritis and diarrhea of the laying hens.
(1) Test materials
Test drugs: the traditional Chinese medicine microecologics prepared in each embodiment.
Test animals: 700 laying hens with 300 days old kalanchoe brown disease are provided by a certain laying hen house in a chat city of Shandong province. The chicken group symptoms are: the water sample is thin manure with blood, and the feed particles in the manure are obvious; the intestinal tract is dissected to swell and bleed, and ulcer focus is formed.
(2) Test method
The experiment adopts single-factor completely random design, and 7 groups are arranged in total, and each group contains 100 diseased laying hens. The test groups 1-5 were fed with drinking water containing the inventive herbal microecological preparation in the same dose as in examples 1-5, and the group 6 was a control group with drinking water containing the comparative herbal preparation in the same dose as in group 3. Group 7 was a blank group fed with feed or drinking water without any drug for 5 days of the test period, and the feeding environment was consistent for each group. The treatment of the affected chickens in each group at the end of the trial was recorded.
Table 1 therapeutic effect of Chinese medicinal micro-ecological preparation added into drinking water on sick laying hens
Figure BDA0002841148780000061
As can be seen from the table 1, after 5 days, the traditional Chinese medicine microecologics are added into the drinking water of the sick chicken, the cure rate of the sick chicken is over 96 percent according to the test groups 1-5, and the cure rate of the control group is 88 percent; 63% of the chickens in the blank group were in a sick state, and the mortality rate reached 19%. The results show that the traditional Chinese medicine microecological preparation for treating enteritis and diarrhea of the laying hens has obvious effect.
Test example 2
The experimental example examines the treatment effect of the traditional Chinese medicine microecologics in example 3 on enteritis and diarrhea of laying hens under the condition of large-group breeding.
(1) Test materials
Test drugs: example 3 preparation of a Chinese medicinal Microecological preparation
Test animals: about 10000 laying hens with the blue brown disease at 330 days old are provided by a certain laying hen farm in Linyi city of Shandong province. The chicken group symptoms are: the feed intake and the laying rate are reduced; the water sample is thin manure with mucus, and the feed particles in the manure are obvious; swelling, bleeding, etc. of the dissected intestinal tract.
(2) Test method
Drinking water containing the Chinese medicinal probiotic of the present invention was fed at the dose of example 3 for a test period of 4 days.
(3) Test results
After the medicine is applied for 24 hours, the water sample feces are obviously reduced, and the feces are changed into paste; after 48 hours, the feces are basically formed and are respectively diarrhea; after 72h, the feces of the chicken flocks are recovered to be normal, and the feed intake is recovered to 80% of the amount before the disease occurs; after 96h, the chicken flocks recovered to be normal, and 17 dead laying hens are tested in the whole process. The test results show that the traditional Chinese medicine microecological preparation prepared by the embodiment has obvious treatment effect on enteritis and diarrhea of the laying hens.
Test example 3
The experimental example examines the treatment effect of the traditional Chinese medicine microecological preparation of example 4 on enteritis and diarrhea of laying hens under the condition of large-group breeding.
(1) Test materials
Test drugs: example 4 preparation of Chinese medicine Microecological preparation
Test animals: about 16000 laying hens with disease of No. 3 farmer age of 280 days are provided by a certain laying hen farm in Zhengzhou city, Henan province. The chicken group symptoms are: the disease is attacked for 2 days, and the feed intake and the laying rate are reduced; the water sample is thin excrement, has mucus and individual hemafecia, and the feed particles in the excrement are obvious; intestinal swelling was dissected and bleeding was isolated.
(2) Test method
Drinking water containing the Chinese medicinal probiotic of the present invention was fed at the dose of example 4 for a test period of 5 days.
(3) Test results
After the medicine is taken for 2 days, the disease condition of chicken flocks is obviously improved, and the excrement turns into paste; 3 days later, the feces are basically formed and diarrhea is caused; after 4 days, the feces of the chicken flocks are recovered to be normal, and the feed intake is recovered to 85 percent before the disease occurs; after 5 days, the chicken flocks recovered to be normal, and 22 laying hens died in the whole experiment. The test results show that the traditional Chinese medicine microecological preparation prepared by the embodiment has obvious treatment effect on enteritis and diarrhea of the laying hens.

Claims (7)

1. A preparation method of a traditional Chinese medicine microecological preparation for treating enteritis and diarrhea of laying hens is characterized by comprising the following steps: soaking the Chinese medicinal decoction piece composition in water, boiling, concentrating to obtain water extractive solution, inoculating Lactobacillus plantarum, and performing anaerobic fermentation; the traditional Chinese medicine decoction piece composition is composed of the following traditional Chinese medicine decoction pieces in parts by weight: 280-320 parts of Chinese pulsatilla root, 280-320 parts of purslane, 130-170 parts of giant knotweed rhizome, 180-220 parts of amur corktree bark, 90-110 parts of rhubarb, 130-170 parts of dark plum fruit, 90-110 parts of myrobalan, 180-220 parts of costus root, 550-650 parts of swordlike atractylodes rhizome, 90-110 parts of lightyellow sophora root, 90-110 parts of pomegranate bark, 90-110 parts of honeysuckle stem, 90-110 parts of common andrographis herb and 45-55 parts of liquorice.
2. The method for preparing the traditional Chinese medicine microecological preparation for treating enteritis and diarrhea of laying hens as claimed in claim 1, wherein the traditional Chinese medicine decoction piece composition is composed of the following traditional Chinese medicine decoction pieces in parts by weight: 300 parts of Chinese pulsatilla root, 300 parts of purslane, 150 parts of giant knotweed, 200 parts of amur corktree bark, 100 parts of rhubarb, 150 parts of dark plum fruit, 100 parts of myrobalan, 200 parts of costus root, 600 parts of swordlike atractylodes rhizome, 100 parts of lightyellow sophora root, 100 parts of pomegranate bark, 100 parts of honeysuckle stem, 100 parts of common andrographis herb and 50 parts of liquoric root.
3. The method for preparing the microecological preparation of Chinese herbs for treating enteritis and diarrhea of laying hens as claimed in claim 1, wherein the weight of the water extract is 1.5-2 times of the weight of the herbal pieces composition, the inoculation amount of lactobacillus plantarum is 1-10% of the weight of the water extract, and the viable count of lactobacillus plantarum is (1.0-20.0) × 10 8 CFU/mL。
4. The method for preparing the Chinese herbal microecological preparation for treating enteritis and diarrhea of laying hens as claimed in claim 3, wherein the lactobacillus plantarum inoculation amount is 7% by weight of the water extract, and the viable count of lactobacillus plantarum is 1.0 x 10 8 CFU/mL。
5. The preparation method of the traditional Chinese medicine microecological preparation for treating enteritis and diarrhea of laying hens according to claim 1, wherein the anaerobic fermentation is carried out at a temperature of 28-37 ℃ for 48-72 hours.
6. The preparation method of the traditional Chinese medicine microecological preparation for treating enteritis and diarrhea of laying hens as claimed in any one of claims 1 to 5, wherein the water adding amount is 5 to 10 times of the traditional Chinese medicine decoction piece composition, the soaking time is 12 to 24 hours, and the boiling time is 2 to 4 hours.
7. The method for preparing the traditional Chinese medicine microecological preparation for treating enteritis and diarrhea of laying hens as claimed in claim 6, wherein the preparation method comprises boiling and extracting with water for 2-3 times, combining the extracts, and then concentrating.
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CN104547126A (en) * 2014-11-28 2015-04-29 河南省农业高新科技园有限公司 Traditional Chinese medicine composition for treating bacterial diarrhea of poultry
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