CN114948805A

CN114948805A - Antibacterial composition containing sweet wormwood herb volatile oil and application of antibacterial composition in acne-removing skin care product

Info

Publication number: CN114948805A
Application number: CN202110204083.6A
Authority: CN
Inventors: 陶侃; 郭莉莉; 杨丹; 胡新成; 李维
Original assignee: Shanghai Kunyao Biotechnology Co ltd
Current assignee: Shanghai Kunyao Biotechnology Co ltd
Priority date: 2021-02-23
Filing date: 2021-02-23
Publication date: 2022-08-30

Abstract

The invention provides a bacteriostatic composition containing sweet wormwood volatile oil and application thereof in acne-removing skin care products, the antibacterial composition comprises herba Artemisiae Annuae volatile oil and o-cymene-5-alcohol, Spanish Salvia officinalis (SALVIA HISPANICA) seed extract, allantoin, dipotassium glycyrrhizinate and other small molecule antibacterial substances, the acne-removing skin care product prepared by compounding the components can complement each other from the aspect of mechanism, meanwhile, the synergistic effect can be achieved, the inhibition activity of the propionibacterium acnes can be effectively improved, the production and the propagation of the propionibacterium acnes can be comprehensively inhibited from different angles such as prevention, diagnosis and treatment, radical treatment and the like, the related inflammation symptoms of acne are reduced, the secretion of skin grease is controlled, the effect of relieving and removing acnes is achieved, and the acne cream is greener, safer, nonirritant, more stable and free of preservatives, so that the comprehensive acne removal effect and the long-term improvement of skin quality are really achieved.

Description

Antibacterial composition containing sweet wormwood herb volatile oil and application of antibacterial composition in acne-removing skin care product

Technical Field

The invention relates to the technical field of cosmetics, and particularly relates to a bacteriostatic composition containing sweet wormwood herb volatile oil and having an acne removing effect and application of the bacteriostatic composition in an acne removing skin care product.

Background

With the influence of factors such as the development of human society, serious environmental pollution, rapid increase of living pressure and the like, the skin problems of urban personnel are gradually exposed, dull, colored spots, acne, comedo, sensitivity and the like, so that the beauty and skin care are more and more emphasized by people, and besides pursuing excellent efficacy, the safety and the stability are also important points of attention of people.

The skin care product compounded by the Chinese herbal medicine raw materials is increasingly accepted and approved by consumers, besides the dependence effect is achieved through chemical components, the Chinese herbal medicine and plant components from natural sources can achieve long-term improvement of skin quality through dredging and nourishing, and the skin care product is safe and stable. For example, the acne is inhibited, when the androgen (mainly testosterone) in the body rises, the 5 alpha-reductase of the hair follicle and the sebaceous gland is converted into dihydrotestosterone with stronger activity, the biochemical reaction is promoted, the sebaceous gland duct and the hair follicle cells are excessively keratinized, and then the sebaceous gland duct or the hair follicle duct is sealed, so that an anaerobic microenvironment which is favorable for the colonization of propionibacterium acnes is formed. Propionibacterium acnes can be degraded easily to form fatty acid with inflammatory factor attribute after growing rapidly, so that acne can be inflamed; meanwhile, as the skin lesion, staphylococcus and malassezia furfur on the surface of the skin participate in related inflammatory reaction, so that the vicious circle is promoted.

At present, the efficacy scheme aiming at acne mainly comprises tretinoin, antibiotics, benzoyl peroxide and the like, the active substances generally have the problems of strong irritation, poor stability, poor permeability or easy promotion of microbial drug resistance and the like, meanwhile, the generation of dependence is easy to cause, and once the active substances are stopped to be used, the effect is difficult to continue and even deteriorates rapidly. The problems of safety and stability are still to be solved by using tretinoin, benzoyl peroxide, antibiotics and the like to prevent and treat acne.

Artemisia apiacea has 2000 years of medicinal history in China, is used for treating hemorrhoids, malaria, and the like in early stage, and the anti-malaria effective monomer compound artemisinin is firstly separated in 1972, so that the method quickly becomes the focus of attention; in the 80 s of the 20 th century, people paid attention to the research of the sweet wormwood volatile oil. The volatile oil of sweet wormwood herb is obtained from the extraction process of artemisinin, and is comprehensively utilized, so that economic loss caused by abandonment is avoided, and the green sustainable development of the cosmetic industry and the whole scientific and technical industry is promoted. And as plant functional components, the sweet wormwood herb volatile oil has a complex spatial structure, whether the effective components really permeate into the horny layer or not is difficult to explain, and whether the effect can be really realized or not is also to be proved.

The o-cymene-5-ol, extract of Spanish sage (SALVIA HISPANICA) seed, allantoin and dipotassium glycyrrhizinate are small molecular substances, and can permeate into stratum corneum to exert antibacterial effect. o-cymene-5-ol as an antifungal preservative effective against the proliferation of propionibacterium acnes; the extract of the seeds of the Spanish sage (SALVIA HISPANICA) is rich in linolenic acid and linoleic acid which are necessary for human bodies, wherein the linolenic acid can play a role in resisting inflammation, and the linoleic acid plays a role in barrier repair and can inhibit the growth of colonies; allantoin has the property of keratolytic protein, so has the effects of softening keratin and promoting epidermal metabolism, and has good wound healing effect on skin lesion caused by acne; the dipotassium glycyrrhizinate can affect ion channels, activate or inhibit enzyme activity, regulate substance metabolism and excitability of cholinergic nerves by acting on hormone receptors (receptors) on cell membranes, so that permeability of capillary walls and the cell membranes is reduced, inflammatory exudation is reduced, formation and release of other toxic substances such as histamine groups and the like are inhibited, and the resistance effect of inflammation caused by permeation of other small-molecule functional components in the skin is enhanced.

At present, no related report exists for preparing an acne-removing skin care product which can achieve the effect of inhibiting acne from different mechanisms and angles by combining the sweet wormwood herb volatile oil and the small molecular substances, so that the skin quality is really improved.

Disclosure of Invention

In order to solve the problems in the prior art, the invention provides a bacteriostatic composition which comprises sweet wormwood herb volatile oil, o-cymene-5-ol, a spanish sage (SALVIA HISPANICA) seed extract, allantoin and other small molecular bacteriostatic substances, and the acne-removing skin care product prepared by compounding the components can effectively improve the inhibitory activity on propionibacterium acnes, achieves a more effective acne-removing effect, can comprehensively inhibit the production and reproduction of propionibacterium acnes from different angles of prevention, diagnosis, treatment, radical treatment and the like, reduces the symptoms of related acne inflammation, controls the secretion of skin oil and fat, and has the effect of relieving acne, so that the long-term improvement of skin quality is realized, and the skin care product is greener, safer, nonirritating and stable and does not need preservatives.

In one aspect, the invention provides a bacteriostatic composition comprising sweet wormwood volatile oil and o-cymene-5-ol.

A large number of experiments prove that the inhibition activity of the compound preparation on propionibacterium acnes can be greatly improved by compounding the sweet wormwood herb volatile oil of the Chinese herbal medicine plant raw materials with the chemical component o-cymene-5-alcohol in a certain proportion, and the synergistic effect exists, so that the effect of inhibiting acnes is achieved from different mechanism angles, and short-term and long-term skin quality improvement is better achieved.

In sebaceous gland cells, androgen can be converted into more active Dihydrotestosterone (DHT) under the action of 5 a-reductase, and further high expression of inflammatory factor IL-6 is stimulated, so that the capacity of inducing acne is enhanced. Therefore, the inhibition of the inflammatory factor IL-6 can inhibit the acne and achieve the effect of treating the acne. The antibacterial composition provided by the invention can also obviously inhibit an inflammatory factor IL-6, so that the effect of inhibiting acne is further improved.

The sweet wormwood volatile oil is obtained by distillation and purification in the extraction process, and is a fraction above 90 ℃.

Further, the bacteriostatic composition further comprises a spanish sage (SALVIA HISPANICA) seed extract.

The extract of Salvia Spanish (SALVIA HISPANICA) seed is rich in linoleic acid and linolenic acid, and has effects of inhibiting colony growth and resisting inflammation. Experiments prove that the antibacterial composition added with the extract of the seeds of the Spanish sage (SALVIA HISPANICA) can further inhibit the expression of inflammatory factors, thereby helping to control acne.

A large number of experiments prove that after the extract of the seeds of the Spanish sage (SALVIA HISPANICA), the inhibition effect on the IL-6 can be further improved.

Further, the bacteriostatic composition also comprises allantoin.

Allantoin is beneficial to epidermal metabolism, promotes keratinocyte regeneration, and helps acne heal and radically cure as soon as possible.

Further, the antibacterial composition also comprises dipotassium glycyrrhizinate.

The dipotassium glycyrrhizinate is used as a relieving component, and can effectively deal with the risk of skin irritation caused by the composition of the functional components.

Further, the antibacterial composition comprises, by mass, 0.1-5 parts of sweet wormwood herb volatile oil, 0.01-0.1 part of o-cymene-5-ol, 0.01-1 part of spanish sage (SALVIA HISPANICA) seed extract, 0.01-0.3 part of allantoin and 0.01-0.3 part of dipotassium glycyrrhizinate.

According to a certain mass ratio, the obtained antibacterial composition can complement each other mechanically, can also realize synergistic interaction, can comprehensively inhibit the production and reproduction of propionibacterium acnes from different angles such as prevention, diagnosis and treatment, radical treatment and the like, reduces inflammation symptoms related to acne, and has the effect of relieving acne.

Further, the antibacterial composition comprises 1 part of sweet wormwood herb volatile oil, 0.1 part of o-cymene-5-ol, 0.1 part of sage spanish (SALVIA HISPANICA) seed extract, 0.2 part of allantoin and 0.2 part of dipotassium glycyrrhizinate in parts by weight.

On the other hand, the invention provides an acne-removing skin care product containing the antibacterial composition.

In some embodiments, the skin care product comprises water, milk, cream, serum, facial mask, facial cleanser, essential oil, and the like.

The acne-removing skin care product containing the antibacterial composition of the present invention may be appropriately blended with various components generally blended in the fields of cosmetics and quasi drugs, such as powder components, moisturizers, thickeners, metal ion masking agents, pigments, pH adjusters, skin nutrients, vitamins, preservatives, antioxidants, antioxidant aids, perfumes, and the like, as needed, within the range that the effects of the present invention are not impaired.

In another aspect, the invention provides an application of the antibacterial composition in preparing acne-removing skin care products.

Further, the preparation method of the acne-removing skin care product comprises the following steps: premixing o-cymene-5-alcohol and dipropylene glycol, and sodium polyacryl dimethyl taurate, adding deionized water, 1, 2-pentanediol, herba Salvia officinalis (SALVIA HISPANICA) seed extract, allantoin and dipotassium glycyrrhizinate, stirring uniformly at 35 deg.C, homogenizing for 5 min, heating to 80-85 deg.C, stirring for 30min, cooling to 45 deg.C, stirring, adding herba Artemisiae Annuae volatile oil, stirring uniformly, cooling, standing, and discharging.

Wherein, the sodium polyacryl dimethyl taurate is used as a thickening agent and is premixed by dipropylene glycol.

In a further aspect, the invention provides the use of the bacteriostatic composition for preparing the propionibacterium acnes inhibitor.

In another aspect, the invention provides the use of the antibacterial composition in the preparation of an inhibitor of inflammatory factor IL-6.

In some embodiments, the bacteriostatic composition provided by the invention can effectively inhibit the inflammatory factor IL-6.

Has the advantages that:

1. the compounding of natural Chinese herbal medicine plant component sweet wormwood herb volatile oil and small molecular chemical substance o-cymene-5-alcohol effectively improves the inhibition activity to propionibacterium acnes.

2. The addition of the extract of the seeds of the Spanish Salvia (SALVIA HISPANICA) can further improve the ability of inhibiting the expression of an inflammatory factor IL-6, avoid the formation of an anaerobic microenvironment beneficial to the breeding of Propionibacterium acnes at sebaceous glands or hair follicle ducts, and help to prevent and radically treat acne.

3. The allantoin and the dipotassium glycyrrhizinate are added to further relieve acne, so that the acne can be healed and cured radically as soon as possible.

4. The antibacterial composition can complement each other mechanically, can also realize synergistic interaction, comprehensively inhibit the production and reproduction of propionibacterium acnes from different angles of prevention, diagnosis and treatment, radical treatment and the like, reduce the symptoms of inflammation related to acne, control the secretion of skin grease, and play a role in relieving acne.

5. Not only solves the problems of safety and irritation, but also has no hidden stability trouble, does not need to add preservative, and really realizes the comprehensive acne removing effect and the long-term improvement of skin.

Drawings

FIG. 1 is a schematic diagram showing the synergistic effect of the volatile oil of Artemisia annua, o-cymene-5-ol and Salvia Spanish (SALVIA HISPANICA) seed extract in inhibiting IL-6 in example 2

FIG. 2 is a schematic diagram showing the effect of the amount of the volatile oil of Artemisia annua in example 3 on the inhibition of IL-6

Fig. 3 is a photograph of the volunteers of example 17 after using the acne-removing skin care product provided by the present invention for 3d, 7d and 14d

Detailed Description

The present invention is described in further detail below with reference to examples, which are intended to facilitate the understanding of the present invention without limiting it in any way. The reagents used in this example were all known products and were obtained by purchasing commercially available products.

The volatile oil of sweet wormwood herb (shanghai kunzi medicine biotechnology limited) provided in this example is distilled and purified in the extraction process to obtain a fraction above 90 ℃; extracts of Spanish Salvia (SALVIA HISPANICA) seeds were purchased from Signum Biosciences, USA.

Example 1 in vitro bacteriostatic test

In this example, the sample concentrations were designed to be

1. o-cymene-5-ol 0.01mg/mL, 0.02mg/mL, 0.05mg/mL, 0.1mg/mL, 0.125mg/mL, 0.2mg/mL, 0.25 mg/mL;

2. 0.02mg/mL, 0.05mg/mL, 0.1mg/mL, 2.5mg/mL, 10mg/mL, 30mg/mL, 50mg/mL of sweet wormwood volatile oil;

3. artemisia apiacea volatile oil and o-cymene-5-alcohol are 0.1mg/mL +0.02mg/mL, 1mg/mL +0.05mg/mL, 2.5mg/mL +0.05mg/mL, 10mg/mL +0.1mg/mL, 30mg/mL +0.125mg/mL, 50mg/mL +0.2 mg/mL.

The three groups of samples are adopted, an agar dilution method is adopted to carry out an antibacterial test, the antibacterial effect of the acne removing raw material on propionibacterium acnes is investigated, and the WS/T639-2018 technical requirement for antibacterial drug susceptibility test (4.2.1) is specifically referred to. The plate was placed on a black, non-reflective background to read the results. MIC was taken as the lowest concentration that inhibited macroscopic growth of bacteria. If the bacterial colony grows, the concentration sample has an antibacterial effect on the propionibacterium acnes, and if the bacterial colony grows, the concentration sample has no antibacterial effect on the propionibacterium acnes. The results are shown in Table 1.

TABLE 1 results of inhibition of colony growth of Propionibacterium acnes by acne-removing materials at different concentrations

The growth of the flat lawn of the growth control group designed by the test is normal, which indicates that the test system is normal and meets the test standard. The Artemisia apiacea volatile oil grows on a plate with a concentration of 1mg/mL or less and on a plate with a concentration of 10mg/mL or more, and the MIC of the Artemisia apiacea volatile oil is 10 mg/mL; the growth of a lawn of o-cymene-5-ol on plates with a concentration of 0.05mg/mL or less and on plates with a concentration of 0.1mg/mL or more showed that the MIC of o-cymene-5-ol was 0.1 mg/mL.

When the sweet wormwood volatile oil and the o-cymene-5-ol are added into the flat plate at the same time, when the concentration of the sweet wormwood volatile oil is lower than MIC10mg/mL and the concentration of the o-cymene-5-ol is lower than MIC 0.1mg/mL, a good antibacterial effect can be achieved, particularly when the concentration of the sweet wormwood volatile oil is as low as 1mg/mL and the concentration of the o-cymene-5-ol is as low as 0.05mg/mL, the sweet wormwood volatile oil and the o-cymene-5-ol still grow aseptically, and therefore the compound of the sweet wormwood volatile oil and the o-cymene-5-ol has a synergistic antibacterial effect and can remarkably improve the antibacterial effect on propionibacterium acnes.

Example 2 synergistic Effect of Artemisia annua volatile oil, o-cymene-5-ol and Salvia Spanish seed (SALVIA HISPANICA) extract in inhibiting IL-6 as an inflammatory factor

Acne is a disease caused by multiple factors, local androgen metabolism is one of the most main causes, and excessive androgen can cause the volume of sebaceous glands to be increased and sebum secretion to be increased, the duct of the pilosebaceous glands is abnormal in keratinization, sebum excretion is not smooth, lipid embolism is formed, and acne is caused. In sebaceous gland cells, androgen can be converted into more active Dihydrotestosterone (DHT) under the action of 5 a-reductase, and further high expression of inflammatory factor IL-6 is stimulated, so that the capacity of inducing acne is enhanced. Therefore, the inhibition of the inflammatory factor IL-6 can inhibit acne, and achieve the effect of treating the acne.

This example compares the inhibition of the inflammatory factor IL-6 with a single component and a composition, respectively.

The main experimental process is as follows: raw264.7 mouse macrophage cells were cultured normally in DMEM medium containing 10% FBS at 37 ℃ in 5% CO 2. When the cells grow to 90% density, inoculating the cells into a 96-well plate according to the density of 8x105/ml, after adhering the cells to the wall for 8 hours, adding samples with different concentrations for respectively treating for 24 hours, and adding corresponding test samples into blank control groups (normal groups), LPS (induction groups), LPS + positive control groups (80 mu M dexamethasone) and LPS + sample groups, wherein the sample groups are respectively 1% of sweet wormwood herb volatile oil; and sweet wormwood volatile oil, o-cymene-5-ol and extract of Spanish sage (SALVIA HISPANICA) seeds, wherein the composition content is 1%, and the composition is prepared from sweet wormwood oil: 0.8%, cymene: 0.1 percent. Spanish sage (SALVIA HISPANICA) seed extract: 0.1 percent of the total saponin is directly mixed, cultured for 24 hours at 37 ℃ and saturated humidity with 5 percent of CO2, and supernatant is taken for detection, and each group is repeated for 3 times. The test results are shown in fig. 1.

As can be seen from FIG. 1, the secretion of inflammatory factor IL-6 by Raw264.7 cells is obviously up-regulated after LPS treatment, and the amount of IL-6 is inhibited after the positive control dexamethasone treatment. The herba Artemisiae Annuae volatile oil has inhibitory effect on inflammatory factor IL-6, but is lower than positive control dexamethasone; to date, there is no report in the literature that o-cymene-5-ol and extract of Salvia Spanish (SALVIA HISPANICA) seed have inhibitory effects on IL-6, an inflammatory factor when used alone; the inhibition rate of the composition compounded by the three components is obviously higher than that of positive control dexamethasone (P is less than 0.01), which shows that the three components of the sweet wormwood herb volatile oil, the o-cymene-5-ol and the spanish sage (SALVIA HISPANICA) seed extract have obvious synergistic effect on the inhibition effect of the inflammatory factor IL-6.

Example 3 Effect of amount of volatile oil of Artemisia annua on the inhibition of IL-6 Effect of inflammatory factor

The preparation method of the acne-removing skin care product containing the bacteriostatic composition provided by the embodiment comprises the following steps: 0.1 part of o-cymene-5-alcohol, 3 parts of dipropylene glycol and 0.3 part of sodium polyacryl dimethyl taurate are premixed, 81.9 parts of deionized water, 3 parts of 1, 2-pentanediol, 0.1 part of salvia officinalis (SALVIA HISPANICA) seed extract, 0.2 part of allantoin and 0.2 part of dipotassium glycyrrhizinate are added, the mixture is stirred uniformly and homogenized for 5 minutes at the temperature of below 35 ℃, the mixture is heated to 80-85 ℃, the mixture is cooled to below 45 ℃ after being stirred for 30 minutes at the temperature, the mixture is continuously stirred and added with sweet wormwood volatile oil, and the mixture is cooled, stood and discharged after being stirred uniformly. Wherein the addition amount of the sweet wormwood volatile oil is 0.05, 0.1, 1 and 2 parts respectively, and the rest is complemented by deionized water.

The main experimental process is as follows: raw264.7 mouse macrophage cells were cultured normally in DMEM medium containing 5% CO2 at 37 ℃ using 10% FBS. When the cells grow to 90% density, inoculating the cells into a 96-well plate according to the density of 8x105/ml, adhering the cells to the wall for 8h, adding samples with different concentrations, respectively treating for 24h, culturing at 37 ℃ and containing 5% CO2 for 24h under saturated humidity, and taking supernatant for detection. Dividing into blank control group (normal group), LPS (induced group), LPS + positive control group (80 μ M dexamethasone), and LPS + sample group, adding corresponding test sample, wherein in the positive control group, 80 μ M dexamethasone is used as positive control, judging whether inflammatory factor IL-6 is inhibited, examining the influence of different addition of composition herba Artemisiae Annuae volatile oil on inhibiting inflammatory factor IL-6 effect, and the detection result is shown in figure 3.

As can be seen from fig. 3, different amounts of the volatile artemisia apiacea oil have a large influence on the expression of the inflammatory factor IL-6, the effect of inhibiting the inflammatory factor IL-6 is gradually increased with the gradual increase of the amount of the volatile artemisia apiacea oil, but when the amount of the volatile artemisia apiacea oil is more than 1 part, the effect of inhibiting the inflammatory factor IL-6 is decreased, and therefore, the amount of the volatile artemisia apiacea oil is preferably 1 part.

Example 4 Artemisia annua volatile oil 1 part + o-cymene-5-ol 0.1 part + Salvia Spanish (SALVIA HISPANICA) seed extract 0.1 part + allantoin 0.2 part + dipotassium glycyrrhizinate 0.2 part

The preparation method of the acne-removing skin care product containing the bacteriostatic composition provided by the embodiment comprises the following steps: 0.1 part of o-cymene-5-alcohol, 3 parts of dipropylene glycol and 0.3 part of sodium polyacryl dimethyl taurate are premixed, 92.1 parts of deionized water, 3 parts of 1, 2-pentanediol, 0.1 part of salvia officinalis (SALVIA HISPANICA) seed extract, 0.2 part of allantoin and 0.2 part of dipotassium glycyrrhizinate are added, the mixture is stirred uniformly and homogenized for 5 minutes at the temperature of below 35 ℃, the mixture is heated to 80-85 ℃, the mixture is cooled to below 45 ℃ after being stirred for 30 minutes at the temperature, the mixture is continuously stirred and added with 1 part of sweet wormwood volatile oil, and the mixture is cooled, stood and discharged after being stirred uniformly.

Example 5 contains no Artemisia annua volatile oil

The difference between the present example and example 4 is that, the acne-removing skin care product containing the bacteriostatic composition is prepared without the volatile oil of artemisia apiacea as follows: 0.1 part of o-cymene-5-alcohol, 3 parts of dipropylene glycol and 0.3 part of sodium polyacryl dimethyl taurate are premixed, 93.1 parts of deionized water, 3 parts of 1, 2-pentanediol, 0.1 part of salvia sclarea (SALVIA HISPANICA) seed extract, 0.2 part of allantoin and 0.2 part of dipotassium glycyrrhizinate are added, the mixture is stirred uniformly and homogenized for 5 minutes at the temperature of below 35 ℃, the mixture is heated to 80-85 ℃, the mixture is kept warm and stirred for 30 minutes, cooled and kept stand, and the material is discharged.

Example 6 No o-cymene-5-ol

The difference between the embodiment and the embodiment 4 is that o-cymene-5-alcohol is not contained, and the acne-removing skin care product containing the antibacterial composition is prepared by the following method: 0.1 part of Spanish sage (SALVIA HISPANICA) seed extract, 0.2 part of allantoin, 0.2 part of dipotassium glycyrrhizinate and 98.5 parts of deionized water are added, the mixture is stirred uniformly at the temperature of below 35 ℃ and homogenized for 5 minutes, then the mixture is heated to 80-85 ℃, the mixture is kept warm and stirred for 30 minutes, then the temperature is reduced to below 45 ℃, the mixture is continuously stirred, 1 part of sweet wormwood herb volatile oil is added, and the mixture is cooled and kept stand after being stirred uniformly, and then the mixture is discharged.

Example 7 contains no Spanish sage (SALVIA HISPANICA) seed extract

The difference between the present example and example 4 is that, without the extract of the seeds of salvia spanish (SALVIA HISPANICA), the acne-removing skin care product containing the bacteriostatic composition is provided by the following preparation method: 0.1 part of o-cymene-5-alcohol, 3 parts of dipropylene glycol and 0.3 part of sodium polyacryloyldimethyl taurate are premixed, 92.2 parts of deionized water, 3 parts of 1, 2-pentanediol, 0.2 part of allantoin and 0.2 part of dipotassium glycyrrhizinate are added, the mixture is stirred uniformly and homogenized for 5 minutes at the temperature of below 35 ℃, the mixture is heated to 80-85 ℃, the mixture is stirred for 30 minutes under heat preservation, the temperature is reduced to below 45 ℃, the mixture is continuously stirred, 1 part of sweet wormwood volatile oil is added, and the mixture is cooled, stood and discharged after being stirred uniformly.

Example 8 No allantoin

The difference between the embodiment and the embodiment 4 is that, without allantoin, the acne-removing skin care product containing the bacteriostatic composition is prepared by the following method: 0.1 part of o-cymene-5-alcohol, 3 parts of dipropylene glycol and 0.3 part of sodium polyacryloyldimethyl taurate are premixed, 92.3 parts of deionized water, 3 parts of 1, 2-pentanediol, 0.1 part of salvia officinalis (SALVIA HISPANICA) seed extract and 0.2 part of dipotassium glycyrrhizinate are added, the mixture is stirred uniformly at the temperature of below 35 ℃ and homogenized for 5 minutes, the mixture is heated to 80-85 ℃, the temperature is kept and stirred for 30 minutes, then the mixture is cooled to the temperature of below 45 ℃, the mixture is continuously stirred, 1 part of sweet wormwood volatile oil is added, and after the mixture is stirred uniformly, the mixture is cooled and kept stand, and the material is discharged.

Example 9 Artemisia annua volatile oil 5 parts + o-cymene-5-ol 0.1 parts + Salvia Spanish (SALVIA HISPANICA) seed extract 0.1 parts + allantoin 0.2 parts + dipotassium glycyrrhizinate 0.2 parts

The difference between the embodiment and the embodiment 4 is that the content of the volatile oil of the sweet wormwood herb is 5 parts, and the preparation method of the acne-removing skin care product containing the antibacterial composition comprises the following steps: 0.1 part of o-cymene-5-alcohol, 3 parts of dipropylene glycol and 0.3 part of sodium polyacryl dimethyl taurate are premixed, 88.1 parts of deionized water, 3 parts of 1, 2-pentanediol, 0.1 part of salvia officinalis (SALVIA HISPANICA) seed extract, 0.2 part of allantoin and 0.2 part of dipotassium glycyrrhizinate are added, the mixture is stirred uniformly and homogenized for 5 minutes at the temperature of below 35 ℃, the mixture is heated to 80-85 ℃, the mixture is cooled to below 45 ℃ after being stirred for 30 minutes, the mixture is continuously stirred and 5 parts of sweet wormwood volatile oil are added, and the mixture is cooled and kept stand after being stirred uniformly and discharged.

Example 10 Artemisia annua volatile oil 1 part + o-cymene-5-ol 0.05 part + Salvia Spanish (SALVIA HISPANICA) seed extract 0.1 part + allantoin 0.2 part + dipotassium glycyrrhizinate 0.2 part

The difference between the embodiment and the embodiment 4 is that the o-cymene-5-alcohol content is 0.05 part, and the preparation method of the acne-removing skin care product containing the antibacterial composition is as follows: 0.05 part of o-cymene-5-alcohol, 3 parts of dipropylene glycol and 0.3 part of sodium polyacryl dimethyl taurate are premixed, 92.15 parts of deionized water, 3 parts of 1, 2-pentanediol, 0.1 part of a sage (SALVIA HISPANICA) seed extract, 0.2 part of allantoin and 0.2 part of dipotassium glycyrrhizinate are added, the mixture is stirred uniformly and homogenized for 5 minutes at the temperature of below 35 ℃, the mixture is heated to 80-85 ℃, the temperature is reduced to below 45 ℃ after the mixture is kept and stirred for 30 minutes, the mixture is continuously stirred and added with 1 part of sweet wormwood volatile oil, and the mixture is cooled, stood and discharged after the mixture is stirred uniformly.

Example 11 Artemisia apiacea volatile oil 1 part + o-cymene-5-ol 0.1 part + Salvia Spanish (SALVIA HISPANICA) seed extract 1 part + allantoin 0.2 part + dipotassium glycyrrhizinate 0.2 part

The difference between the present example and example 4 is that the content of the spanish sage (SALVIA HISPANICA) seed extract is 1 part, and the preparation method of the acne-removing skin care product containing the bacteriostatic composition is as follows: 0.1 part of o-cymene-5-alcohol, 3 parts of dipropylene glycol and 0.3 part of sodium polyacryl dimethyl taurate are premixed, 91.2 parts of deionized water, 3 parts of 1, 2-pentanediol, 1 part of salvia officinalis (SALVIA HISPANICA) seed extract, 0.2 part of allantoin and 0.2 part of dipotassium glycyrrhizinate are added, the mixture is stirred uniformly and homogenized for 5 minutes at the temperature of below 35 ℃, the mixture is heated to 80-85 ℃, the temperature is reduced to below 45 ℃ after the mixture is kept for stirring for 30 minutes, the mixture is continuously stirred and 1 part of sweet wormwood volatile oil is added, and the mixture is cooled, stood and discharged after being stirred uniformly.

Example 12 Artemisia annua volatile oil 1 part + o-cymene-5-ol 0.1 part + Salvia Spanish (SALVIA HISPANICA) seed extract 0.1 part + allantoin 0.05 part + dipotassium glycyrrhizinate 0.2 part

The difference between the embodiment and the embodiment 4 is that the allantoin content is 0.05 part, and the preparation method of the acne-removing skin care product containing the antibacterial composition is as follows: 0.1 part of o-cymene-5-alcohol, 3 parts of dipropylene glycol and 0.3 part of sodium polyacryl dimethyl taurate are premixed, 92.25 parts of deionized water, 3 parts of 1, 2-pentanediol, 0.1 part of salvia officinalis (SALVIA HISPANICA) seed extract, 0.05 part of allantoin and 0.2 part of dipotassium glycyrrhizinate are added, the mixture is stirred uniformly and homogenized for 5 minutes at the temperature of below 35 ℃, the mixture is heated to 80-85 ℃, the mixture is cooled to below 45 ℃ after being stirred for 30 minutes at the temperature, the mixture is continuously stirred and added with 1 part of sweet wormwood volatile oil, and the mixture is cooled, stood and discharged after being stirred uniformly.

Example 13 Artemisia annua volatile oil 1 part + o-cymene-5-ol 0.1 part + Salvia Spanish (SALVIA HISPANICA) seed extract 0.1 part + allantoin 0.2 part + dipotassium glycyrrhizinate 0.05 part

The difference between the embodiment and the embodiment 4 is that the content of dipotassium glycyrrhizinate is 0.05 part, and the preparation method of the acne-removing skin care product containing the antibacterial composition is as follows: 0.1 part of o-cymene-5-alcohol, 3 parts of dipropylene glycol and 0.3 part of sodium polyacryl dimethyl taurate are premixed, 92.25 parts of deionized water, 3 parts of 1, 2-pentanediol, 0.1 part of salvia officinalis (SALVIA HISPANICA) seed extract, 0.2 part of allantoin and 0.05 part of dipotassium glycyrrhizinate are added, the mixture is stirred uniformly and homogenized for 5 minutes at the temperature of below 35 ℃, the mixture is heated to 80-85 ℃, the mixture is cooled to below 45 ℃ after being stirred for 30 minutes at the temperature, the mixture is continuously stirred and added with 1 part of sweet wormwood volatile oil, and the mixture is cooled, stood and discharged after being stirred uniformly.

Example 14 stability test

In this embodiment, the stability test of the acne-removing skin care products prepared in examples 4 to 13 specifically includes: the stability test of high temperature 40 ℃, low temperature-4 ℃, centrifugation 2000-4000r/min x 30min is carried out, whether the appearance shapes such as thinning, color change, layering, hardness change and the like exist or not is observed by naked eyes, whether the odor change exists or not is judged by olfaction, and the stability test result is shown in table 2.

Table 2 stability test results

Examples	High temperature survey	Low temperature investigation	Centrifugal investigation
				3	Stabilization of	Stabilization	Stabilization of
4	Stabilization of	Stabilization	Stabilization
				5	Stabilization	Stabilization	Stabilization
6	Stabilization	Stabilization	Stabilization of
				7	Stabilization	Stabilization	Stabilization
8	Stabilization of	Stabilization	Stabilization
				9	Stabilization	Stabilization	Stabilization
10	Stabilization	Stabilization	Stabilization of
				11	Stabilization	Stabilization of	Stabilization
12	Stabilization	Stabilization	Stabilization

As can be seen from Table 2, the acne-removing skin care products prepared in examples 4-13 are stable at the conditions of high temperature of 40 ℃, low temperature of-4 ℃, and centrifugation of 2000-4000r/min x 30min, and the stability completely meets the requirements.

Example 15 safety test

One, multiple (acute) skin irritation test

In this example, the acne-removing skin care products prepared in examples 4 to 13 were subjected to multiple (acute) skin irritation tests, and animal experiments were performed according to the "technical specification for cosmetics" 2015 edition, wherein at least 4 adult, healthy, and skin-intact white rabbits were used as test animals. Before the test, the hair on both sides of the spine of the test animal is cut off, the hair removing range is respectively 3cm multiplied by 3cm, and the smearing area is 2.5cm multiplied by 2.5 cm. About 0.5g of the test substance was applied to one side of the skin 1 time per day for 14 days. From the next day, shearing hair before each application, removing residual test substance with water or non-irritant solvent, observing the result after one hour, and recording the result and intensity grading according to the skin irritation reaction standard in the current effective technical specification. The results of multiple (acute) skin irritation tests are shown in table 5.

Second, acute eye irritation test

In this example, the acne-removing skin care products prepared in examples 4 to 13 were subjected to acute eye irritation test by animal experiments according to 2015, which is the "technical specification for cosmetics", and at least 3 adult and healthy white rabbits were used as test animals. 0.1g of the test substance was instilled (or applied) into the conjunctival sac, and the upper and lower eyelids were passively closed for 1s, while the other eye was left untreated as a self-control. The eyes of the animals were examined 1/24/48/72h and 4d and 7d after instillation of the test substance. If no stimulus response occurs for 72 hours, the test is terminated. If the patient does not recover within 7 days after discovering involvement of the cornea or other eye irritation, the observation time needs to be prolonged to determine the reversibility or irreversibility of the damage, generally not exceeding 21 days, and the result and response grading are recorded according to the eye damage standard in the current effective technical specification. The results of the acute eye irritation test are shown in table 3.

Three, human body skin patch test

In this example, the skin patch test of human body was performed on the acne-removing skin care products prepared in examples 4 to 13, referring to "technical safety standards for cosmetics" 2015 edition, 34 persons in total were selected from the skin care products of 19 to 60 years old and the average age was 41.09 ± 2.15 years old, and then qualified spot test devices were selected, and by the closed spot patch test method, about 0.020 to 0.025g of the test object was placed in a spot tester, and then the test object was externally applied to the back of the subject with hypoallergenic tape, and after 24 hours, the test object was removed, and then the skin reactions were observed at 0.5, 24, and 48 hours after removal, and the results were recorded according to the skin reaction grading standards in the current effective standards. The results of the human skin patch test are shown in table 4.

TABLE 3 safety test results animal experiments

TABLE 4 safety test results-human skin Patch test

As can be seen from tables 3 and 4, the acne-removing skin care products prepared in examples 4 to 13 have no irritation or different skin reactions in the multiple (acute) skin irritation tests, acute eye irritation tests and human skin patch tests, and therefore, the safety completely meets the requirements.

Example 16 Corrosion protection challenge test

This example performed a preservative challenge Test on the anti-acne skin care products prepared in examples 4-13, with reference to German Shumei corporation standard SM021 "KoKoKoKoKo Test" (28 days for single inoculation, the inoculation numbers of bacteria, yeasts and molds were 10 respectively ⁸ germs/ml), the preservative challenge test was performed on each example for a 4 week visit period. Evaluation criteria of resultsComprises the following steps: -means no growth (< 10) ² germs/ml), + indicates slight growth (10) ² -10 ³ germs/ml), + + indicates moderate growth (10) ³ -10 ⁴ germs/ml), +++ indicate severe growth (> 10) ⁵ germs/ml). The test results are shown in table 5.

TABLE 5 Corrosion protection challenge test results for each example

As can be seen from table 5, the acne-removing skin care product provided by the invention has a remarkable antibacterial effect due to the compound combination of the sweet wormwood herb volatile oil and cymene, does not need to be added with a preservative, and is more green, safe and healthy.

Example 17 acne treatment efficacy test

In this example, the skin care products prepared in examples 4 to 13 were tested for anti-acne efficacy, and 300 healthy and pox-affected volunteers were recruited and screened and randomly divided into 10 groups of 30 persons each, and each of examples 3 to 12 was tested. The age of the volunteers is mainly concentrated on 18-30 years, and 77% of the volunteers have more than 5 pox growing amount per month. Any cosmetics or external medicines can not be used for 2-3 days before the tested part. Before the experiment, the volunteers needed to clean their faces. In the experiment, each group of volunteers applied the corresponding sample 2 times in the morning and evening, each time according to the dosage of 1mg/cm 2. After 3d, 7d and 14d were used, questionnaires were investigated and photographs were taken using a facial imaging instrument ViSia, taking a set of photographs see fig. 3. The results of the acne treatment are shown in table 6.

TABLE 6 questionnaire on anti-acne efficacy of human skin

As can be seen from table 6, the acne removing effects of examples 3 and 8 are the best, and the higher the concentration of the oil containing artemisia apiacea is, the better the effect is; however, the sweet wormwood volatile oil has strong pungent and cool characteristic smell and is pungent, so the best combination mode is selected in the embodiment 3.

Although the present invention is disclosed above, the present invention is not limited thereto. Various changes and modifications may be effected therein by one skilled in the art without departing from the spirit and scope of the invention as defined in the appended claims.

Claims

1. An antibacterial composition, which is characterized by comprising sweet wormwood volatile oil and o-cymene-5-alcohol.

2. Bacteriostatic composition according to claim 1, further comprising a sage seed extract from Spanish.

3. The bacteriostatic composition according to claim 1 or 2, further comprising allantoin.

4. The bacteriostatic composition according to claim 3, further comprising dipotassium glycyrrhizinate.

5. Bacteriostatic composition according to claim 4, wherein the bacteriostatic composition comprises 0.1-5 parts by weight of Artemisia annua volatile oil, 0.01-0.1 part by weight of o-cymene-5-ol, 0.01-1 part by weight of Salvia Spanish seed extract, 0.01-0.3 part by weight of allantoin and 0.01-0.3 part by weight of dipotassium glycyrrhizinate.

6. An acne-removing skin care product containing the bacteriostatic composition as defined in any one of claims 1-5.

7. Use of the bacteriostatic composition according to any one of claims 1-5 in preparation of acne-removing skin care products.

8. The use of claim 7, wherein the acne-removing skin care product is prepared by a method comprising the following steps: premixing o-cymene-5-alcohol and dipropylene glycol, and sodium polyacryl dimethyl taurate, adding deionized water, 1, 2-pentanediol, Spanish sage seed extract, allantoin and dipotassium glycyrrhizinate, stirring uniformly at 35 deg.C, homogenizing for 5 min, heating to 80-85 deg.C, stirring at constant temperature for 30min, cooling to 45 deg.C, stirring, adding herba Artemisiae Annuae volatile oil, stirring uniformly, cooling, standing, and discharging.

9. Use of a bacteriostatic composition according to any one of claims 1-5 in the preparation of a propionibacterium acnes inhibitor.

10. Use of a bacteriostatic composition according to any one of claims 1-5 in the preparation of an inhibitor of the inflammatory factor IL-6.