CN114921513B - 一种乳酸菌与嗜冷菌蛋白酶组合固态发酵制备火麻蛋白肽的方法 - Google Patents
一种乳酸菌与嗜冷菌蛋白酶组合固态发酵制备火麻蛋白肽的方法 Download PDFInfo
- Publication number
- CN114921513B CN114921513B CN202210132689.8A CN202210132689A CN114921513B CN 114921513 B CN114921513 B CN 114921513B CN 202210132689 A CN202210132689 A CN 202210132689A CN 114921513 B CN114921513 B CN 114921513B
- Authority
- CN
- China
- Prior art keywords
- lactobacillus
- cannabis
- protein peptide
- seed meal
- protease
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 112
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 83
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 83
- 238000010563 solid-state fermentation Methods 0.000 title claims abstract description 41
- 239000004365 Protease Substances 0.000 title claims abstract description 40
- 108091005804 Peptidases Proteins 0.000 title claims abstract description 39
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 title claims abstract description 23
- 241000894006 Bacteria Species 0.000 title claims abstract description 20
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 title claims description 30
- 239000004310 lactic acid Substances 0.000 title claims description 15
- 235000014655 lactic acid Nutrition 0.000 title claims description 15
- 240000004308 marijuana Species 0.000 title 1
- 235000012054 meals Nutrition 0.000 claims abstract description 98
- 241000218236 Cannabis Species 0.000 claims abstract description 86
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 34
- 241000186660 Lactobacillus Species 0.000 claims abstract description 32
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 32
- 238000000605 extraction Methods 0.000 claims abstract description 25
- 238000000855 fermentation Methods 0.000 claims abstract description 25
- 230000004151 fermentation Effects 0.000 claims abstract description 25
- 238000002360 preparation method Methods 0.000 claims abstract description 24
- 102000004190 Enzymes Human genes 0.000 claims abstract description 17
- 108090000790 Enzymes Proteins 0.000 claims abstract description 17
- 238000004108 freeze drying Methods 0.000 claims abstract description 10
- 239000007787 solid Substances 0.000 claims abstract description 10
- 238000011081 inoculation Methods 0.000 claims abstract description 6
- 244000025254 Cannabis sativa Species 0.000 claims description 100
- 235000012766 Cannabis sativa ssp. sativa var. sativa Nutrition 0.000 claims description 93
- 235000012765 Cannabis sativa ssp. sativa var. spontanea Nutrition 0.000 claims description 93
- 235000009120 camo Nutrition 0.000 claims description 93
- 235000005607 chanvre indien Nutrition 0.000 claims description 93
- 239000011487 hemp Substances 0.000 claims description 93
- 239000000843 powder Substances 0.000 claims description 52
- 239000007858 starting material Substances 0.000 claims description 46
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 43
- 241000186869 Lactobacillus salivarius Species 0.000 claims description 38
- 239000001963 growth medium Substances 0.000 claims description 36
- 239000000284 extract Substances 0.000 claims description 31
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 19
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 19
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 19
- 230000001954 sterilising effect Effects 0.000 claims description 19
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 18
- 230000003213 activating effect Effects 0.000 claims description 18
- 239000008103 glucose Substances 0.000 claims description 18
- 235000015097 nutrients Nutrition 0.000 claims description 18
- 239000006228 supernatant Substances 0.000 claims description 16
- 241001052560 Thallis Species 0.000 claims description 13
- 238000000108 ultra-filtration Methods 0.000 claims description 13
- 239000000287 crude extract Substances 0.000 claims description 12
- 230000009849 deactivation Effects 0.000 claims description 11
- 239000012528 membrane Substances 0.000 claims description 11
- 238000003756 stirring Methods 0.000 claims description 11
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 10
- 239000002054 inoculum Substances 0.000 claims description 10
- 238000004659 sterilization and disinfection Methods 0.000 claims description 10
- 239000001888 Peptone Substances 0.000 claims description 9
- 108010080698 Peptones Proteins 0.000 claims description 9
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 9
- 235000015278 beef Nutrition 0.000 claims description 9
- 229940041514 candida albicans extract Drugs 0.000 claims description 9
- 238000012258 culturing Methods 0.000 claims description 9
- 239000008367 deionised water Substances 0.000 claims description 9
- 229910021641 deionized water Inorganic materials 0.000 claims description 9
- YXVFQADLFFNVDS-UHFFFAOYSA-N diammonium citrate Chemical compound [NH4+].[NH4+].[O-]C(=O)CC(O)(C(=O)O)CC([O-])=O YXVFQADLFFNVDS-UHFFFAOYSA-N 0.000 claims description 9
- 238000002386 leaching Methods 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 9
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 9
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 claims description 9
- 235000019319 peptone Nutrition 0.000 claims description 9
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 9
- 229920000053 polysorbate 80 Polymers 0.000 claims description 9
- 239000001632 sodium acetate Substances 0.000 claims description 9
- 235000017281 sodium acetate Nutrition 0.000 claims description 9
- 239000008223 sterile water Substances 0.000 claims description 9
- 239000012138 yeast extract Substances 0.000 claims description 9
- 229910052799 carbon Inorganic materials 0.000 claims description 8
- 235000008697 Cannabis sativa Nutrition 0.000 claims description 7
- 239000013049 sediment Substances 0.000 claims description 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
- 235000010855 food raising agent Nutrition 0.000 claims 1
- 229920001184 polypeptide Polymers 0.000 abstract description 31
- 235000019658 bitter taste Nutrition 0.000 abstract description 10
- 238000002156 mixing Methods 0.000 abstract description 8
- 238000009776 industrial production Methods 0.000 abstract description 4
- 102000004400 Aminopeptidases Human genes 0.000 abstract description 3
- 108090000915 Aminopeptidases Proteins 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 3
- 230000008569 process Effects 0.000 abstract description 3
- 230000000813 microbial effect Effects 0.000 abstract description 2
- 244000005700 microbiome Species 0.000 abstract description 2
- 230000015556 catabolic process Effects 0.000 abstract 2
- 238000006731 degradation reaction Methods 0.000 abstract 2
- 150000004676 glycans Chemical class 0.000 abstract 1
- 239000002207 metabolite Substances 0.000 abstract 1
- 229920001282 polysaccharide Polymers 0.000 abstract 1
- 239000005017 polysaccharide Substances 0.000 abstract 1
- 230000017854 proteolysis Effects 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 description 71
- 235000019419 proteases Nutrition 0.000 description 27
- 229940088598 enzyme Drugs 0.000 description 13
- 238000004519 manufacturing process Methods 0.000 description 11
- 239000003921 oil Substances 0.000 description 10
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 7
- 239000012535 impurity Substances 0.000 description 7
- 230000003647 oxidation Effects 0.000 description 7
- 238000007254 oxidation reaction Methods 0.000 description 7
- 239000002244 precipitate Substances 0.000 description 7
- 150000001413 amino acids Chemical class 0.000 description 5
- 241000589540 Pseudomonas fluorescens Species 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 230000001360 synchronised effect Effects 0.000 description 4
- 235000001014 amino acid Nutrition 0.000 description 3
- VPZXBVLAVMBEQI-UHFFFAOYSA-N glycyl-DL-alpha-alanine Natural products OC(=O)C(C)NC(=O)CN VPZXBVLAVMBEQI-UHFFFAOYSA-N 0.000 description 3
- BEMGNWZECGIJOI-WDSKDSINSA-N Ala-Gly-Glu Chemical compound [H]N[C@@H](C)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(O)=O BEMGNWZECGIJOI-WDSKDSINSA-N 0.000 description 2
- BLIMFWGRQKRCGT-YUMQZZPRSA-N Ala-Gly-Lys Chemical compound C[C@H](N)C(=O)NCC(=O)N[C@H](C(O)=O)CCCCN BLIMFWGRQKRCGT-YUMQZZPRSA-N 0.000 description 2
- FNXCAFKDGBROCU-STECZYCISA-N Arg-Ile-Tyr Chemical compound NC(N)=NCCC[C@H](N)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@H](C(O)=O)CC1=CC=C(O)C=C1 FNXCAFKDGBROCU-STECZYCISA-N 0.000 description 2
- TVVYVAUGRHNTGT-UGYAYLCHSA-N Asp-Asp-Ile Chemical compound CC[C@H](C)[C@@H](C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](N)CC(O)=O TVVYVAUGRHNTGT-UGYAYLCHSA-N 0.000 description 2
- UMHUHHJMEXNSIV-CIUDSAMLSA-N Asp-Leu-Ser Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CC(O)=O UMHUHHJMEXNSIV-CIUDSAMLSA-N 0.000 description 2
- 240000001929 Lactobacillus brevis Species 0.000 description 2
- 235000013957 Lactobacillus brevis Nutrition 0.000 description 2
- 241000186840 Lactobacillus fermentum Species 0.000 description 2
- CIIJWIAORKTXAH-FJXKBIBVSA-N Met-Thr-Gly Chemical compound CSCC[C@H](N)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(O)=O CIIJWIAORKTXAH-FJXKBIBVSA-N 0.000 description 2
- 108010006035 Metalloproteases Proteins 0.000 description 2
- 102000005741 Metalloproteases Human genes 0.000 description 2
- SBVPYBFMIGDIDX-SRVKXCTJSA-N Pro-Pro-Pro Chemical compound OC(=O)[C@@H]1CCCN1C(=O)[C@H]1N(C(=O)[C@H]2NCCC2)CCC1 SBVPYBFMIGDIDX-SRVKXCTJSA-N 0.000 description 2
- XPNSAQMEAVSQRD-FBCQKBJTSA-N Thr-Gly-Gly Chemical compound C[C@@H](O)[C@H](N)C(=O)NCC(=O)NCC(O)=O XPNSAQMEAVSQRD-FBCQKBJTSA-N 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 230000007071 enzymatic hydrolysis Effects 0.000 description 2
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 2
- 108010050848 glycylleucine Proteins 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 229940012969 lactobacillus fermentum Drugs 0.000 description 2
- 108010034529 leucyl-lysine Proteins 0.000 description 2
- 108010057821 leucylproline Proteins 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 108010051242 phenylalanylserine Proteins 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000011343 solid material Substances 0.000 description 2
- 230000003068 static effect Effects 0.000 description 2
- RLMISHABBKUNFO-WHFBIAKZSA-N Ala-Ala-Gly Chemical compound C[C@H](N)C(=O)N[C@@H](C)C(=O)NCC(O)=O RLMISHABBKUNFO-WHFBIAKZSA-N 0.000 description 1
- MCKSLROAGSDNFC-ACZMJKKPSA-N Ala-Asp-Gln Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCC(N)=O)C(O)=O MCKSLROAGSDNFC-ACZMJKKPSA-N 0.000 description 1
- ZIWWTZWAKYBUOB-CIUDSAMLSA-N Ala-Asp-Leu Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(O)=O ZIWWTZWAKYBUOB-CIUDSAMLSA-N 0.000 description 1
- ZVFVBBGVOILKPO-WHFBIAKZSA-N Ala-Gly-Ala Chemical compound C[C@H](N)C(=O)NCC(=O)N[C@@H](C)C(O)=O ZVFVBBGVOILKPO-WHFBIAKZSA-N 0.000 description 1
- WGDNWOMKBUXFHR-BQBZGAKWSA-N Ala-Gly-Arg Chemical compound C[C@H](N)C(=O)NCC(=O)N[C@H](C(O)=O)CCCN=C(N)N WGDNWOMKBUXFHR-BQBZGAKWSA-N 0.000 description 1
- PCIFXPRIFWKWLK-YUMQZZPRSA-N Ala-Gly-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)CNC(=O)[C@H](C)N PCIFXPRIFWKWLK-YUMQZZPRSA-N 0.000 description 1
- SUMYEVXWCAYLLJ-GUBZILKMSA-N Ala-Leu-Gln Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O SUMYEVXWCAYLLJ-GUBZILKMSA-N 0.000 description 1
- VGMNWQOPSFBBBG-XUXIUFHCSA-N Ala-Leu-Leu-Asp Chemical compound C[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(O)=O VGMNWQOPSFBBBG-XUXIUFHCSA-N 0.000 description 1
- MEFILNJXAVSUTO-JXUBOQSCSA-N Ala-Leu-Thr Chemical compound C[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O MEFILNJXAVSUTO-JXUBOQSCSA-N 0.000 description 1
- PVQLRJRPUTXFFX-CIUDSAMLSA-N Ala-Met-Gln Chemical compound CSCC[C@H](NC(=O)[C@H](C)N)C(=O)N[C@@H](CCC(N)=O)C(O)=O PVQLRJRPUTXFFX-CIUDSAMLSA-N 0.000 description 1
- XSTZMVAYYCJTNR-DCAQKATOSA-N Ala-Met-Leu Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(C)C)C(O)=O XSTZMVAYYCJTNR-DCAQKATOSA-N 0.000 description 1
- YYAVDNKUWLAFCV-ACZMJKKPSA-N Ala-Ser-Gln Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(N)=O)C(O)=O YYAVDNKUWLAFCV-ACZMJKKPSA-N 0.000 description 1
- AOAKQKVICDWCLB-UWJYBYFXSA-N Ala-Tyr-Asn Chemical compound C[C@@H](C(=O)N[C@@H](CC1=CC=C(C=C1)O)C(=O)N[C@@H](CC(=O)N)C(=O)O)N AOAKQKVICDWCLB-UWJYBYFXSA-N 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- ZTKHZAXGTFXUDD-VEVYYDQMSA-N Arg-Asn-Thr Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O ZTKHZAXGTFXUDD-VEVYYDQMSA-N 0.000 description 1
- XVLLUZMFSAYKJV-GUBZILKMSA-N Arg-Asp-Arg Chemical compound NC(N)=NCCC[C@H](N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCN=C(N)N)C(O)=O XVLLUZMFSAYKJV-GUBZILKMSA-N 0.000 description 1
- KMSHNDWHPWXPEC-BQBZGAKWSA-N Arg-Asp-Gly Chemical compound NC(N)=NCCC[C@H](N)C(=O)N[C@@H](CC(O)=O)C(=O)NCC(O)=O KMSHNDWHPWXPEC-BQBZGAKWSA-N 0.000 description 1
- RKRSYHCNPFGMTA-CIUDSAMLSA-N Arg-Glu-Asn Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O RKRSYHCNPFGMTA-CIUDSAMLSA-N 0.000 description 1
- IYMAXBFPHPZYIK-BQBZGAKWSA-N Arg-Gly-Asp Chemical compound NC(N)=NCCC[C@H](N)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(O)=O IYMAXBFPHPZYIK-BQBZGAKWSA-N 0.000 description 1
- NKNILFJYKKHBKE-WPRPVWTQSA-N Arg-Gly-Val Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](C(C)C)C(O)=O NKNILFJYKKHBKE-WPRPVWTQSA-N 0.000 description 1
- PRLPSDIHSRITSF-UNQGMJICSA-N Arg-Phe-Thr Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H]([C@@H](C)O)C(O)=O PRLPSDIHSRITSF-UNQGMJICSA-N 0.000 description 1
- OVQJAKFLFTZDNC-GUBZILKMSA-N Arg-Pro-Asp Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(O)=O)C(O)=O OVQJAKFLFTZDNC-GUBZILKMSA-N 0.000 description 1
- WCZXPVPHUMYLMS-VEVYYDQMSA-N Arg-Thr-Asp Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(O)=O)C(O)=O WCZXPVPHUMYLMS-VEVYYDQMSA-N 0.000 description 1
- CPTXATAOUQJQRO-GUBZILKMSA-N Arg-Val-Ser Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CO)C(O)=O CPTXATAOUQJQRO-GUBZILKMSA-N 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- QEYJFBMTSMLPKZ-ZKWXMUAHSA-N Asn-Ala-Val Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](C(C)C)C(O)=O QEYJFBMTSMLPKZ-ZKWXMUAHSA-N 0.000 description 1
- PCKRJVZAQZWNKM-WHFBIAKZSA-N Asn-Asn-Gly Chemical compound NC(=O)C[C@H](N)C(=O)N[C@@H](CC(N)=O)C(=O)NCC(O)=O PCKRJVZAQZWNKM-WHFBIAKZSA-N 0.000 description 1
- IOTKDTZEEBZNCM-UGYAYLCHSA-N Asn-Asn-Ile Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O IOTKDTZEEBZNCM-UGYAYLCHSA-N 0.000 description 1
- KXEGPPNPXOKKHK-ZLUOBGJFSA-N Asn-Asp-Ala Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](C)C(O)=O KXEGPPNPXOKKHK-ZLUOBGJFSA-N 0.000 description 1
- CUQUEHYSSFETRD-ACZMJKKPSA-N Asn-Asp-Gln Chemical compound C(CC(=O)N)[C@@H](C(=O)O)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CC(=O)N)N CUQUEHYSSFETRD-ACZMJKKPSA-N 0.000 description 1
- XQQVCUIBGYFKDC-OLHMAJIHSA-N Asn-Asp-Thr Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O XQQVCUIBGYFKDC-OLHMAJIHSA-N 0.000 description 1
- IYVSIZAXNLOKFQ-BYULHYEWSA-N Asn-Asp-Val Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](C(C)C)C(O)=O IYVSIZAXNLOKFQ-BYULHYEWSA-N 0.000 description 1
- CTQIOCMSIJATNX-WHFBIAKZSA-N Asn-Gly-Ala Chemical compound [H]N[C@@H](CC(N)=O)C(=O)NCC(=O)N[C@@H](C)C(O)=O CTQIOCMSIJATNX-WHFBIAKZSA-N 0.000 description 1
- OLVIPTLKNSAYRJ-YUMQZZPRSA-N Asn-Gly-Lys Chemical compound C(CCN)C[C@@H](C(=O)O)NC(=O)CNC(=O)[C@H](CC(=O)N)N OLVIPTLKNSAYRJ-YUMQZZPRSA-N 0.000 description 1
- CBWCQCANJSGUOH-ZKWXMUAHSA-N Asn-Val-Ala Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](C)C(O)=O CBWCQCANJSGUOH-ZKWXMUAHSA-N 0.000 description 1
- QHAJMRDEWNAIBQ-FXQIFTODSA-N Asp-Arg-Asn Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(N)=O)C(O)=O QHAJMRDEWNAIBQ-FXQIFTODSA-N 0.000 description 1
- CASGONAXMZPHCK-FXQIFTODSA-N Asp-Asn-Arg Chemical compound C(C[C@@H](C(=O)O)NC(=O)[C@H](CC(=O)N)NC(=O)[C@H](CC(=O)O)N)CN=C(N)N CASGONAXMZPHCK-FXQIFTODSA-N 0.000 description 1
- WLKVEEODTPQPLI-ACZMJKKPSA-N Asp-Gln-Asn Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O WLKVEEODTPQPLI-ACZMJKKPSA-N 0.000 description 1
- HRGGPWBIMIQANI-GUBZILKMSA-N Asp-Gln-Leu Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(O)=O HRGGPWBIMIQANI-GUBZILKMSA-N 0.000 description 1
- OMMIEVATLAGRCK-BYPYZUCNSA-N Asp-Gly-Gly Chemical compound OC(=O)C[C@H](N)C(=O)NCC(=O)NCC(O)=O OMMIEVATLAGRCK-BYPYZUCNSA-N 0.000 description 1
- KTTCQQNRRLCIBC-GHCJXIJMSA-N Asp-Ile-Ala Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C)C(O)=O KTTCQQNRRLCIBC-GHCJXIJMSA-N 0.000 description 1
- PYXXJFRXIYAESU-PCBIJLKTSA-N Asp-Ile-Phe Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O PYXXJFRXIYAESU-PCBIJLKTSA-N 0.000 description 1
- RQHLMGCXCZUOGT-ZPFDUUQYSA-N Asp-Leu-Ile Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O RQHLMGCXCZUOGT-ZPFDUUQYSA-N 0.000 description 1
- JUWISGAGWSDGDH-KKUMJFAQSA-N Asp-Phe-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)CC(O)=O)CC1=CC=CC=C1 JUWISGAGWSDGDH-KKUMJFAQSA-N 0.000 description 1
- USNJAPJZSGTTPX-XVSYOHENSA-N Asp-Phe-Thr Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H]([C@@H](C)O)C(O)=O USNJAPJZSGTTPX-XVSYOHENSA-N 0.000 description 1
- GPPIDDWYKJPRES-YDHLFZDLSA-N Asp-Phe-Val Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](C(C)C)C(O)=O GPPIDDWYKJPRES-YDHLFZDLSA-N 0.000 description 1
- ZQFRDAZBTSFGGW-SRVKXCTJSA-N Asp-Ser-Phe Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O ZQFRDAZBTSFGGW-SRVKXCTJSA-N 0.000 description 1
- JSNWZMFSLIWAHS-HJGDQZAQSA-N Asp-Thr-Leu Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)O)NC(=O)[C@H](CC(=O)O)N)O JSNWZMFSLIWAHS-HJGDQZAQSA-N 0.000 description 1
- GXHDGYOXPNQCKM-XVSYOHENSA-N Asp-Thr-Phe Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)O)NC(=O)[C@H](CC(=O)O)N)O GXHDGYOXPNQCKM-XVSYOHENSA-N 0.000 description 1
- 108090000145 Bacillolysin Proteins 0.000 description 1
- MTNJRNQDDSWQQA-GQGQLFGLSA-N Cys-Ile-Trp Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CC1=CNC2=CC=CC=C21)C(=O)O)NC(=O)[C@H](CS)N MTNJRNQDDSWQQA-GQGQLFGLSA-N 0.000 description 1
- KHGGWBRVRPHFMH-PEFMBERDSA-N Gln-Ile-Asn Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)O)NC(=O)[C@H](CCC(=O)N)N KHGGWBRVRPHFMH-PEFMBERDSA-N 0.000 description 1
- FTIJVMLAGRAYMJ-MNXVOIDGSA-N Gln-Ile-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@@H](N)CCC(N)=O FTIJVMLAGRAYMJ-MNXVOIDGSA-N 0.000 description 1
- JNENSVNAUWONEZ-GUBZILKMSA-N Gln-Lys-Asn Chemical compound [H]N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(N)=O)C(O)=O JNENSVNAUWONEZ-GUBZILKMSA-N 0.000 description 1
- RONJIBWTGKVKFY-HTUGSXCWSA-N Gln-Thr-Phe Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)O)NC(=O)[C@H](CCC(=O)N)N)O RONJIBWTGKVKFY-HTUGSXCWSA-N 0.000 description 1
- WIMVKDYAKRAUCG-IHRRRGAJSA-N Gln-Tyr-Glu Chemical compound C1=CC(=CC=C1C[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)O)NC(=O)[C@H](CCC(=O)N)N)O WIMVKDYAKRAUCG-IHRRRGAJSA-N 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
- 102000006395 Globulins Human genes 0.000 description 1
- FYBSCGZLICNOBA-XQXXSGGOSA-N Glu-Ala-Thr Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O FYBSCGZLICNOBA-XQXXSGGOSA-N 0.000 description 1
- SBCYJMOOHUDWDA-NUMRIWBASA-N Glu-Asp-Thr Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O SBCYJMOOHUDWDA-NUMRIWBASA-N 0.000 description 1
- CUPSDFQZTVVTSK-GUBZILKMSA-N Glu-Lys-Asp Chemical compound OC(=O)C[C@@H](C(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](N)CCC(O)=O CUPSDFQZTVVTSK-GUBZILKMSA-N 0.000 description 1
- MFNUFCFRAZPJFW-JYJNAYRXSA-N Glu-Lys-Phe Chemical compound OC(=O)CC[C@H](N)C(=O)N[C@@H](CCCCN)C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 MFNUFCFRAZPJFW-JYJNAYRXSA-N 0.000 description 1
- FMBWLLMUPXTXFC-SDDRHHMPSA-N Glu-Lys-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(=O)O)N)C(=O)O FMBWLLMUPXTXFC-SDDRHHMPSA-N 0.000 description 1
- PMSMKNYRZCKVMC-DRZSPHRISA-N Glu-Phe-Ala Chemical compound C[C@@H](C(=O)O)NC(=O)[C@H](CC1=CC=CC=C1)NC(=O)[C@H](CCC(=O)O)N PMSMKNYRZCKVMC-DRZSPHRISA-N 0.000 description 1
- BDISFWMLMNBTGP-NUMRIWBASA-N Glu-Thr-Asp Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(O)=O)C(O)=O BDISFWMLMNBTGP-NUMRIWBASA-N 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- BRFJMRSRMOMIMU-WHFBIAKZSA-N Gly-Ala-Asn Chemical compound NCC(=O)N[C@@H](C)C(=O)N[C@@H](CC(N)=O)C(O)=O BRFJMRSRMOMIMU-WHFBIAKZSA-N 0.000 description 1
- OGCIHJPYKVSMTE-YUMQZZPRSA-N Gly-Arg-Glu Chemical compound [H]NCC(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(O)=O OGCIHJPYKVSMTE-YUMQZZPRSA-N 0.000 description 1
- RJIVPOXLQFJRTG-LURJTMIESA-N Gly-Arg-Gly Chemical compound OC(=O)CNC(=O)[C@@H](NC(=O)CN)CCCN=C(N)N RJIVPOXLQFJRTG-LURJTMIESA-N 0.000 description 1
- LURCIJSJAKFCRO-QWRGUYRKSA-N Gly-Asn-Tyr Chemical compound [H]NCC(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(O)=O LURCIJSJAKFCRO-QWRGUYRKSA-N 0.000 description 1
- JNGJGFMFXREJNF-KBPBESRZSA-N Gly-Glu-Trp Chemical compound [H]NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(O)=O JNGJGFMFXREJNF-KBPBESRZSA-N 0.000 description 1
- PDAWDNVHMUKWJR-ZETCQYMHSA-N Gly-Gly-His Chemical compound NCC(=O)NCC(=O)N[C@H](C(O)=O)CC1=CNC=N1 PDAWDNVHMUKWJR-ZETCQYMHSA-N 0.000 description 1
- CQIIXEHDSZUSAG-QWRGUYRKSA-N Gly-His-His Chemical compound C([C@H](NC(=O)CN)C(=O)N[C@@H](CC=1NC=NC=1)C(O)=O)C1=CN=CN1 CQIIXEHDSZUSAG-QWRGUYRKSA-N 0.000 description 1
- UTYGDAHJBBDPBA-BYULHYEWSA-N Gly-Ile-Asp Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)O)NC(=O)CN UTYGDAHJBBDPBA-BYULHYEWSA-N 0.000 description 1
- HMHRTKOWRUPPNU-RCOVLWMOSA-N Gly-Ile-Gly Chemical compound NCC(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(O)=O HMHRTKOWRUPPNU-RCOVLWMOSA-N 0.000 description 1
- NSTUFLGQJCOCDL-UWVGGRQHSA-N Gly-Leu-Arg Chemical compound NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C(O)=O)CCCN=C(N)N NSTUFLGQJCOCDL-UWVGGRQHSA-N 0.000 description 1
- LOEANKRDMMVOGZ-YUMQZZPRSA-N Gly-Lys-Asp Chemical compound NCCCC[C@H](NC(=O)CN)C(=O)N[C@@H](CC(O)=O)C(O)=O LOEANKRDMMVOGZ-YUMQZZPRSA-N 0.000 description 1
- PDUHNKAFQXQNLH-ZETCQYMHSA-N Gly-Lys-Gly Chemical compound NCCCC[C@H](NC(=O)CN)C(=O)NCC(O)=O PDUHNKAFQXQNLH-ZETCQYMHSA-N 0.000 description 1
- NTBOEZICHOSJEE-YUMQZZPRSA-N Gly-Lys-Ser Chemical compound [H]NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(O)=O NTBOEZICHOSJEE-YUMQZZPRSA-N 0.000 description 1
- MTBIKIMYHUWBRX-QWRGUYRKSA-N Gly-Phe-Asn Chemical compound C1=CC=C(C=C1)C[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)O)NC(=O)CN MTBIKIMYHUWBRX-QWRGUYRKSA-N 0.000 description 1
- ZLCLYFGMKFCDCN-XPUUQOCRSA-N Gly-Ser-Val Chemical compound CC(C)[C@H](NC(=O)[C@H](CO)NC(=O)CN)C(O)=O ZLCLYFGMKFCDCN-XPUUQOCRSA-N 0.000 description 1
- UIQGJYUEQDOODF-KWQFWETISA-N Gly-Tyr-Ala Chemical compound OC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)CN)CC1=CC=C(O)C=C1 UIQGJYUEQDOODF-KWQFWETISA-N 0.000 description 1
- DUAWRXXTOQOECJ-JSGCOSHPSA-N Gly-Tyr-Val Chemical compound [H]NCC(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](C(C)C)C(O)=O DUAWRXXTOQOECJ-JSGCOSHPSA-N 0.000 description 1
- GWCJMBNBFYBQCV-XPUUQOCRSA-N Gly-Val-Ala Chemical compound NCC(=O)N[C@@H](C(C)C)C(=O)N[C@@H](C)C(O)=O GWCJMBNBFYBQCV-XPUUQOCRSA-N 0.000 description 1
- VOKCBYNCZVSILJ-KKUMJFAQSA-N His-Asn-Tyr Chemical compound C1=CC(=CC=C1C[C@@H](C(=O)O)NC(=O)[C@H](CC(=O)N)NC(=O)[C@H](CC2=CN=CN2)N)O VOKCBYNCZVSILJ-KKUMJFAQSA-N 0.000 description 1
- ZJSMFRTVYSLKQU-DJFWLOJKSA-N His-Asp-Ile Chemical compound CC[C@H](C)[C@@H](C(=O)O)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CC1=CN=CN1)N ZJSMFRTVYSLKQU-DJFWLOJKSA-N 0.000 description 1
- LMMPTUVWHCFTOT-GARJFASQSA-N His-Asp-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CC(=O)O)NC(=O)[C@H](CC2=CN=CN2)N)C(=O)O LMMPTUVWHCFTOT-GARJFASQSA-N 0.000 description 1
- FIMNVXRZGUAGBI-AVGNSLFASA-N His-Glu-Leu Chemical compound [H]N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(O)=O FIMNVXRZGUAGBI-AVGNSLFASA-N 0.000 description 1
- OEROYDLRVAYIMQ-YUMQZZPRSA-N His-Gly-Asp Chemical compound [H]N[C@@H](CC1=CNC=N1)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(O)=O OEROYDLRVAYIMQ-YUMQZZPRSA-N 0.000 description 1
- IWXMHXYOACDSIA-PYJNHQTQSA-N His-Ile-Val Chemical compound [H]N[C@@H](CC1=CNC=N1)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C(C)C)C(O)=O IWXMHXYOACDSIA-PYJNHQTQSA-N 0.000 description 1
- BKOVCRUIXDIWFV-IXOXFDKPSA-N His-Lys-Thr Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](N)CC1=CN=CN1 BKOVCRUIXDIWFV-IXOXFDKPSA-N 0.000 description 1
- NBWATNYAUVSAEQ-ZEILLAHLSA-N His-Thr-Thr Chemical compound C[C@H]([C@@H](C(=O)N[C@@H]([C@@H](C)O)C(=O)O)NC(=O)[C@H](CC1=CN=CN1)N)O NBWATNYAUVSAEQ-ZEILLAHLSA-N 0.000 description 1
- YPWHUFAAMNHMGS-QSFUFRPTSA-N Ile-Ala-His Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](C)C(=O)N[C@@H](CC1=CN=CN1)C(=O)O)N YPWHUFAAMNHMGS-QSFUFRPTSA-N 0.000 description 1
- LPFBXFILACZHIB-LAEOZQHASA-N Ile-Gly-Glu Chemical compound CC[C@H](C)[C@@H](C(=O)NCC(=O)N[C@@H](CCC(=O)O)C(=O)O)N LPFBXFILACZHIB-LAEOZQHASA-N 0.000 description 1
- PFPUFNLHBXKPHY-HTFCKZLJSA-N Ile-Ile-Ser Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CO)C(=O)O)N PFPUFNLHBXKPHY-HTFCKZLJSA-N 0.000 description 1
- FZWVCYCYWCLQDH-NHCYSSNCSA-N Ile-Leu-Gly Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)O)N FZWVCYCYWCLQDH-NHCYSSNCSA-N 0.000 description 1
- UIEZQYNXCYHMQS-BJDJZHNGSA-N Ile-Lys-Ala Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)O)N UIEZQYNXCYHMQS-BJDJZHNGSA-N 0.000 description 1
- XQLGNKLSPYCRMZ-HJWJTTGWSA-N Ile-Phe-Val Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](C(C)C)C(=O)O)N XQLGNKLSPYCRMZ-HJWJTTGWSA-N 0.000 description 1
- FQYQMFCIJNWDQZ-CYDGBPFRSA-N Ile-Pro-Pro Chemical compound CC[C@H](C)[C@H](N)C(=O)N1CCC[C@H]1C(=O)N1[C@H](C(O)=O)CCC1 FQYQMFCIJNWDQZ-CYDGBPFRSA-N 0.000 description 1
- JZNVOBUNTWNZPW-GHCJXIJMSA-N Ile-Ser-Asp Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(=O)O)C(=O)O)N JZNVOBUNTWNZPW-GHCJXIJMSA-N 0.000 description 1
- VGSPNSSCMOHRRR-BJDJZHNGSA-N Ile-Ser-Lys Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)O)N VGSPNSSCMOHRRR-BJDJZHNGSA-N 0.000 description 1
- RMJWFINHACYKJI-SIUGBPQLSA-N Ile-Tyr-Glu Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CC1=CC=C(C=C1)O)C(=O)N[C@@H](CCC(=O)O)C(=O)O)N RMJWFINHACYKJI-SIUGBPQLSA-N 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
- KKXDHFKZWKLYGB-GUBZILKMSA-N Leu-Asn-Glu Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCC(=O)O)C(=O)O)N KKXDHFKZWKLYGB-GUBZILKMSA-N 0.000 description 1
- FGNQZXKVAZIMCI-CIUDSAMLSA-N Leu-Asp-Cys Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CS)C(=O)O)N FGNQZXKVAZIMCI-CIUDSAMLSA-N 0.000 description 1
- BABSVXFGKFLIGW-UWVGGRQHSA-N Leu-Gly-Arg Chemical compound CC(C)C[C@H](N)C(=O)NCC(=O)N[C@H](C(O)=O)CCCNC(N)=N BABSVXFGKFLIGW-UWVGGRQHSA-N 0.000 description 1
- HYIFFZAQXPUEAU-QWRGUYRKSA-N Leu-Gly-Leu Chemical compound CC(C)C[C@H](N)C(=O)NCC(=O)N[C@H](C(O)=O)CC(C)C HYIFFZAQXPUEAU-QWRGUYRKSA-N 0.000 description 1
- XBCWOTOCBXXJDG-BZSNNMDCSA-N Leu-His-Phe Chemical compound C([C@H](NC(=O)[C@@H](N)CC(C)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(O)=O)C1=CN=CN1 XBCWOTOCBXXJDG-BZSNNMDCSA-N 0.000 description 1
- JLWZLIQRYCTYBD-IHRRRGAJSA-N Leu-Lys-Arg Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O JLWZLIQRYCTYBD-IHRRRGAJSA-N 0.000 description 1
- RZXLZBIUTDQHJQ-SRVKXCTJSA-N Leu-Lys-Asp Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(O)=O)C(O)=O RZXLZBIUTDQHJQ-SRVKXCTJSA-N 0.000 description 1
- LZHJZLHSRGWBBE-IHRRRGAJSA-N Leu-Lys-Val Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(O)=O LZHJZLHSRGWBBE-IHRRRGAJSA-N 0.000 description 1
- INCJJHQRZGQLFC-KBPBESRZSA-N Leu-Phe-Gly Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)NCC(O)=O INCJJHQRZGQLFC-KBPBESRZSA-N 0.000 description 1
- IRMLZWSRWSGTOP-CIUDSAMLSA-N Leu-Ser-Ala Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@@H](C)C(O)=O IRMLZWSRWSGTOP-CIUDSAMLSA-N 0.000 description 1
- XOWMDXHFSBCAKQ-SRVKXCTJSA-N Leu-Ser-Leu Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@H](C(O)=O)CC(C)C XOWMDXHFSBCAKQ-SRVKXCTJSA-N 0.000 description 1
- VDIARPPNADFEAV-WEDXCCLWSA-N Leu-Thr-Gly Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(O)=O VDIARPPNADFEAV-WEDXCCLWSA-N 0.000 description 1
- HQVDJTYKCMIWJP-YUMQZZPRSA-N Lys-Asn-Gly Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CC(N)=O)C(=O)NCC(O)=O HQVDJTYKCMIWJP-YUMQZZPRSA-N 0.000 description 1
- QZONCCHVHCOBSK-YUMQZZPRSA-N Lys-Gly-Asn Chemical compound [H]N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(O)=O QZONCCHVHCOBSK-YUMQZZPRSA-N 0.000 description 1
- NKKFVJRLCCUJNA-QWRGUYRKSA-N Lys-Gly-Lys Chemical compound NCCCC[C@H](N)C(=O)NCC(=O)N[C@H](C(O)=O)CCCCN NKKFVJRLCCUJNA-QWRGUYRKSA-N 0.000 description 1
- IUWMQCZOTYRXPL-ZPFDUUQYSA-N Lys-Ile-Asp Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(O)=O)C(O)=O IUWMQCZOTYRXPL-ZPFDUUQYSA-N 0.000 description 1
- UQJOKDAYFULYIX-AVGNSLFASA-N Lys-Pro-Pro Chemical compound NCCCC[C@H](N)C(=O)N1CCC[C@H]1C(=O)N1[C@H](C(O)=O)CCC1 UQJOKDAYFULYIX-AVGNSLFASA-N 0.000 description 1
- DIBZLYZXTSVGLN-CIUDSAMLSA-N Lys-Ser-Ser Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(O)=O DIBZLYZXTSVGLN-CIUDSAMLSA-N 0.000 description 1
- ACYHZNZHIZWLQF-BQBZGAKWSA-N Met-Asn-Gly Chemical compound CSCC[C@H](N)C(=O)N[C@@H](CC(N)=O)C(=O)NCC(O)=O ACYHZNZHIZWLQF-BQBZGAKWSA-N 0.000 description 1
- 108010079364 N-glycylalanine Proteins 0.000 description 1
- 108010047562 NGR peptide Proteins 0.000 description 1
- 102000035092 Neutral proteases Human genes 0.000 description 1
- 108091005507 Neutral proteases Proteins 0.000 description 1
- 108090000526 Papain Proteins 0.000 description 1
- DDYIRGBOZVKRFR-AVGNSLFASA-N Phe-Asp-Glu Chemical compound C1=CC=C(C=C1)C[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CCC(=O)O)C(=O)O)N DDYIRGBOZVKRFR-AVGNSLFASA-N 0.000 description 1
- YYKZDTVQHTUKDW-RYUDHWBXSA-N Phe-Gly-Gln Chemical compound C1=CC=C(C=C1)C[C@@H](C(=O)NCC(=O)N[C@@H](CCC(=O)N)C(=O)O)N YYKZDTVQHTUKDW-RYUDHWBXSA-N 0.000 description 1
- OKQQWSNUSQURLI-JYJNAYRXSA-N Phe-Met-Val Chemical compound CC(C)[C@@H](C(=O)O)NC(=O)[C@H](CCSC)NC(=O)[C@H](CC1=CC=CC=C1)N OKQQWSNUSQURLI-JYJNAYRXSA-N 0.000 description 1
- BPCLGWHVPVTTFM-QWRGUYRKSA-N Phe-Ser-Gly Chemical compound [H]N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CO)C(=O)NCC(O)=O BPCLGWHVPVTTFM-QWRGUYRKSA-N 0.000 description 1
- 108010064851 Plant Proteins Proteins 0.000 description 1
- SWXSLPHTJVAWDF-VEVYYDQMSA-N Pro-Asn-Thr Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O SWXSLPHTJVAWDF-VEVYYDQMSA-N 0.000 description 1
- SGCZFWSQERRKBD-BQBZGAKWSA-N Pro-Asp-Gly Chemical compound OC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@@H]1CCCN1 SGCZFWSQERRKBD-BQBZGAKWSA-N 0.000 description 1
- FRKBNXCFJBPJOL-GUBZILKMSA-N Pro-Glu-Glu Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(O)=O FRKBNXCFJBPJOL-GUBZILKMSA-N 0.000 description 1
- YTWNSIDWAFSEEI-RWMBFGLXSA-N Pro-His-Pro Chemical compound C1C[C@H](NC1)C(=O)N[C@@H](CC2=CN=CN2)C(=O)N3CCC[C@@H]3C(=O)O YTWNSIDWAFSEEI-RWMBFGLXSA-N 0.000 description 1
- YXHYJEPDKSYPSQ-AVGNSLFASA-N Pro-Leu-Arg Chemical compound NC(N)=NCCC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H]1CCCN1 YXHYJEPDKSYPSQ-AVGNSLFASA-N 0.000 description 1
- SUENWIFTSTWUKD-AVGNSLFASA-N Pro-Leu-Val Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(O)=O SUENWIFTSTWUKD-AVGNSLFASA-N 0.000 description 1
- ZZCJYPLMOPTZFC-SRVKXCTJSA-N Pro-Met-Met Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCSC)C(O)=O ZZCJYPLMOPTZFC-SRVKXCTJSA-N 0.000 description 1
- RCYUBVHMVUHEBM-RCWTZXSCSA-N Pro-Pro-Thr Chemical compound [H]N1CCC[C@H]1C(=O)N1CCC[C@H]1C(=O)N[C@@H]([C@@H](C)O)C(O)=O RCYUBVHMVUHEBM-RCWTZXSCSA-N 0.000 description 1
- GMJDSFYVTAMIBF-FXQIFTODSA-N Pro-Ser-Asp Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(O)=O GMJDSFYVTAMIBF-FXQIFTODSA-N 0.000 description 1
- KHRLUIPIMIQFGT-AVGNSLFASA-N Pro-Val-Leu Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O KHRLUIPIMIQFGT-AVGNSLFASA-N 0.000 description 1
- HQTKVSCNCDLXSX-BQBZGAKWSA-N Ser-Arg-Gly Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(O)=O HQTKVSCNCDLXSX-BQBZGAKWSA-N 0.000 description 1
- UGJRQLURDVGULT-LKXGYXEUSA-N Ser-Asn-Thr Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O UGJRQLURDVGULT-LKXGYXEUSA-N 0.000 description 1
- TYYBJUYSTWJHGO-ZKWXMUAHSA-N Ser-Asn-Val Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](C(C)C)C(O)=O TYYBJUYSTWJHGO-ZKWXMUAHSA-N 0.000 description 1
- MMAPOBOTRUVNKJ-ZLUOBGJFSA-N Ser-Asp-Ser Chemical compound C([C@@H](C(=O)N[C@@H](CO)C(=O)O)NC(=O)[C@H](CO)N)C(=O)O MMAPOBOTRUVNKJ-ZLUOBGJFSA-N 0.000 description 1
- HEQPKICPPDOSIN-SRVKXCTJSA-N Ser-Asp-Tyr Chemical compound OC[C@H](N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@H](C(O)=O)CC1=CC=C(O)C=C1 HEQPKICPPDOSIN-SRVKXCTJSA-N 0.000 description 1
- QKQDTEYDEIJPNK-GUBZILKMSA-N Ser-Glu-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](N)CO QKQDTEYDEIJPNK-GUBZILKMSA-N 0.000 description 1
- SFTZWNJFZYOLBD-ZDLURKLDSA-N Ser-Gly-Thr Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)CNC(=O)[C@@H](N)CO SFTZWNJFZYOLBD-ZDLURKLDSA-N 0.000 description 1
- SFTZTYBXIXLRGQ-JBDRJPRFSA-N Ser-Ile-Ala Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C)C(O)=O SFTZTYBXIXLRGQ-JBDRJPRFSA-N 0.000 description 1
- YMDNFPNTIPQMJP-NAKRPEOUSA-N Ser-Ile-Met Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCSC)C(O)=O YMDNFPNTIPQMJP-NAKRPEOUSA-N 0.000 description 1
- NLOAIFSWUUFQFR-CIUDSAMLSA-N Ser-Leu-Asp Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(O)=O NLOAIFSWUUFQFR-CIUDSAMLSA-N 0.000 description 1
- WGDYNRCOQRERLZ-KKUMJFAQSA-N Ser-Lys-Phe Chemical compound C1=CC=C(C=C1)C[C@@H](C(=O)O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CO)N WGDYNRCOQRERLZ-KKUMJFAQSA-N 0.000 description 1
- DINQYZRMXGWWTG-GUBZILKMSA-N Ser-Pro-Pro Chemical compound OC[C@H](N)C(=O)N1CCC[C@H]1C(=O)N1[C@H](C(O)=O)CCC1 DINQYZRMXGWWTG-GUBZILKMSA-N 0.000 description 1
- FLONGDPORFIVQW-XGEHTFHBSA-N Ser-Pro-Thr Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](N)CO FLONGDPORFIVQW-XGEHTFHBSA-N 0.000 description 1
- NADLKBTYNKUJEP-KATARQTJSA-N Ser-Thr-Leu Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(C)C)C(O)=O NADLKBTYNKUJEP-KATARQTJSA-N 0.000 description 1
- ZSDXEKUKQAKZFE-XAVMHZPKSA-N Ser-Thr-Pro Chemical compound C[C@H]([C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@H](CO)N)O ZSDXEKUKQAKZFE-XAVMHZPKSA-N 0.000 description 1
- ZWSZBWAFDZRBNM-UBHSHLNASA-N Ser-Trp-Ser Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N[C@@H](CO)C(O)=O ZWSZBWAFDZRBNM-UBHSHLNASA-N 0.000 description 1
- ZVBCMFDJIMUELU-BZSNNMDCSA-N Ser-Tyr-Phe Chemical compound C1=CC=C(C=C1)C[C@@H](C(=O)O)NC(=O)[C@H](CC2=CC=C(C=C2)O)NC(=O)[C@H](CO)N ZVBCMFDJIMUELU-BZSNNMDCSA-N 0.000 description 1
- LGIMRDKGABDMBN-DCAQKATOSA-N Ser-Val-Lys Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CO)N LGIMRDKGABDMBN-DCAQKATOSA-N 0.000 description 1
- GLQFKOVWXPPFTP-VEVYYDQMSA-N Thr-Arg-Asp Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(O)=O GLQFKOVWXPPFTP-VEVYYDQMSA-N 0.000 description 1
- TZKPNGDGUVREEB-FOHZUACHSA-N Thr-Asn-Gly Chemical compound C[C@@H](O)[C@H](N)C(=O)N[C@@H](CC(N)=O)C(=O)NCC(O)=O TZKPNGDGUVREEB-FOHZUACHSA-N 0.000 description 1
- JBHMLZSKIXMVFS-XVSYOHENSA-N Thr-Asn-Phe Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O JBHMLZSKIXMVFS-XVSYOHENSA-N 0.000 description 1
- DCCGCVLVVSAJFK-NUMRIWBASA-N Thr-Asp-Gln Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CCC(=O)N)C(=O)O)N)O DCCGCVLVVSAJFK-NUMRIWBASA-N 0.000 description 1
- UHBPFYOQQPFKQR-JHEQGTHGSA-N Thr-Gln-Gly Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCC(N)=O)C(=O)NCC(O)=O UHBPFYOQQPFKQR-JHEQGTHGSA-N 0.000 description 1
- ONNSECRQFSTMCC-XKBZYTNZSA-N Thr-Glu-Ser Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(O)=O ONNSECRQFSTMCC-XKBZYTNZSA-N 0.000 description 1
- KCRQEJSKXAIULJ-FJXKBIBVSA-N Thr-Gly-Arg Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CCCNC(N)=N)C(O)=O KCRQEJSKXAIULJ-FJXKBIBVSA-N 0.000 description 1
- VTVVYQOXJCZVEB-WDCWCFNPSA-N Thr-Leu-Glu Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(O)=O VTVVYQOXJCZVEB-WDCWCFNPSA-N 0.000 description 1
- WRQLCVIALDUQEQ-UNQGMJICSA-N Thr-Phe-Arg Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O WRQLCVIALDUQEQ-UNQGMJICSA-N 0.000 description 1
- PZSDPRBZINDEJV-HTUGSXCWSA-N Thr-Phe-Gln Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CCC(N)=O)C(O)=O PZSDPRBZINDEJV-HTUGSXCWSA-N 0.000 description 1
- KERCOYANYUPLHJ-XGEHTFHBSA-N Thr-Pro-Ser Chemical compound C[C@@H](O)[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CO)C(O)=O KERCOYANYUPLHJ-XGEHTFHBSA-N 0.000 description 1
- BCYUHPXBHCUYBA-CUJWVEQBSA-N Thr-Ser-His Chemical compound C[C@@H](O)[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@@H](Cc1cnc[nH]1)C(O)=O BCYUHPXBHCUYBA-CUJWVEQBSA-N 0.000 description 1
- GRIUMVXCJDKVPI-IZPVPAKOSA-N Thr-Thr-Tyr Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(O)=O GRIUMVXCJDKVPI-IZPVPAKOSA-N 0.000 description 1
- ZNFPUOSTMUMUDR-JRQIVUDYSA-N Tyr-Asn-Thr Chemical compound [H]N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O ZNFPUOSTMUMUDR-JRQIVUDYSA-N 0.000 description 1
- HSBZWINKRYZCSQ-KKUMJFAQSA-N Tyr-Lys-Asp Chemical compound [H]N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(O)=O)C(O)=O HSBZWINKRYZCSQ-KKUMJFAQSA-N 0.000 description 1
- JLKVWTICWVWGSK-JYJNAYRXSA-N Tyr-Lys-Glu Chemical compound OC(=O)CC[C@@H](C(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](N)CC1=CC=C(O)C=C1 JLKVWTICWVWGSK-JYJNAYRXSA-N 0.000 description 1
- QVYFTFIBKCDHIE-ACRUOGEOSA-N Tyr-Tyr-Lys Chemical compound C1=CC(=CC=C1C[C@@H](C(=O)N[C@@H](CC2=CC=C(C=C2)O)C(=O)N[C@@H](CCCCN)C(=O)O)N)O QVYFTFIBKCDHIE-ACRUOGEOSA-N 0.000 description 1
- DNOOLPROHJWCSQ-RCWTZXSCSA-N Val-Arg-Thr Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H]([C@@H](C)O)C(O)=O DNOOLPROHJWCSQ-RCWTZXSCSA-N 0.000 description 1
- JLFKWDAZBRYCGX-ZKWXMUAHSA-N Val-Asn-Ser Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CO)C(=O)O)N JLFKWDAZBRYCGX-ZKWXMUAHSA-N 0.000 description 1
- HZYOWMGWKKRMBZ-BYULHYEWSA-N Val-Asp-Asp Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC(=O)O)C(=O)O)N HZYOWMGWKKRMBZ-BYULHYEWSA-N 0.000 description 1
- OVLIFGQSBSNGHY-KKHAAJSZSA-N Val-Asp-Thr Chemical compound C[C@H]([C@@H](C(=O)O)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](C(C)C)N)O OVLIFGQSBSNGHY-KKHAAJSZSA-N 0.000 description 1
- CELJCNRXKZPTCX-XPUUQOCRSA-N Val-Gly-Ala Chemical compound CC(C)[C@H](N)C(=O)NCC(=O)N[C@@H](C)C(O)=O CELJCNRXKZPTCX-XPUUQOCRSA-N 0.000 description 1
- PMDOQZFYGWZSTK-LSJOCFKGSA-N Val-Gly-Ile Chemical compound CC[C@H](C)[C@@H](C(O)=O)NC(=O)CNC(=O)[C@@H](N)C(C)C PMDOQZFYGWZSTK-LSJOCFKGSA-N 0.000 description 1
- BTWMICVCQLKKNR-DCAQKATOSA-N Val-Leu-Ser Chemical compound CC(C)[C@H]([NH3+])C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C([O-])=O BTWMICVCQLKKNR-DCAQKATOSA-N 0.000 description 1
- IEBGHUMBJXIXHM-AVGNSLFASA-N Val-Lys-Met Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCSC)C(=O)O)N IEBGHUMBJXIXHM-AVGNSLFASA-N 0.000 description 1
- HPANGHISDXDUQY-ULQDDVLXSA-N Val-Lys-Phe Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)O)N HPANGHISDXDUQY-ULQDDVLXSA-N 0.000 description 1
- RYHUIHUOYRNNIE-NRPADANISA-N Val-Ser-Gln Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(=O)N)C(=O)O)N RYHUIHUOYRNNIE-NRPADANISA-N 0.000 description 1
- QTPQHINADBYBNA-DCAQKATOSA-N Val-Ser-Lys Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@H](C(O)=O)CCCCN QTPQHINADBYBNA-DCAQKATOSA-N 0.000 description 1
- QHSSPPHOHJSTML-HOCLYGCPSA-N Val-Trp-Gly Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CC1=CNC2=CC=CC=C21)C(=O)NCC(=O)O)N QHSSPPHOHJSTML-HOCLYGCPSA-N 0.000 description 1
- 108010041407 alanylaspartic acid Proteins 0.000 description 1
- 108010047495 alanylglycine Proteins 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 108010072041 arginyl-glycyl-aspartic acid Proteins 0.000 description 1
- 108010069926 arginyl-glycyl-serine Proteins 0.000 description 1
- 108010038850 arginyl-isoleucyl-tyrosine Proteins 0.000 description 1
- 108010060035 arginylproline Proteins 0.000 description 1
- 108010069205 aspartyl-phenylalanine Proteins 0.000 description 1
- 108010047857 aspartylglycine Proteins 0.000 description 1
- 108010092854 aspartyllysine Proteins 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 108010009297 diglycyl-histidine Proteins 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 108010055341 glutamyl-glutamic acid Proteins 0.000 description 1
- 108010049041 glutamylalanine Proteins 0.000 description 1
- XBGGUPMXALFZOT-UHFFFAOYSA-N glycyl-L-tyrosine hemihydrate Natural products NCC(=O)NC(C(O)=O)CC1=CC=C(O)C=C1 XBGGUPMXALFZOT-UHFFFAOYSA-N 0.000 description 1
- 108010027668 glycyl-alanyl-valine Proteins 0.000 description 1
- XKUKSGPZAADMRA-UHFFFAOYSA-N glycyl-glycyl-glycine Natural products NCC(=O)NCC(=O)NCC(O)=O XKUKSGPZAADMRA-UHFFFAOYSA-N 0.000 description 1
- 108010089804 glycyl-threonine Proteins 0.000 description 1
- 108010048994 glycyl-tyrosyl-alanine Proteins 0.000 description 1
- 108010010147 glycylglutamine Proteins 0.000 description 1
- 108010015792 glycyllysine Proteins 0.000 description 1
- 108010087823 glycyltyrosine Proteins 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 239000000413 hydrolysate Substances 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 108010031424 isoleucyl-prolyl-proline Proteins 0.000 description 1
- 108010003700 lysyl aspartic acid Proteins 0.000 description 1
- 108010064235 lysylglycine Proteins 0.000 description 1
- 108010038320 lysylphenylalanine Proteins 0.000 description 1
- 230000027939 micturition Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 229940055729 papain Drugs 0.000 description 1
- 235000019834 papain Nutrition 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 108010070409 phenylalanyl-glycyl-glycine Proteins 0.000 description 1
- 235000021118 plant-derived protein Nutrition 0.000 description 1
- 108010025826 prolyl-leucyl-arginine Proteins 0.000 description 1
- 108010077112 prolyl-proline Proteins 0.000 description 1
- 108010004914 prolylarginine Proteins 0.000 description 1
- 108010070643 prolylglutamic acid Proteins 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 108010048818 seryl-histidine Proteins 0.000 description 1
- 235000019640 taste Nutrition 0.000 description 1
- 108010061238 threonyl-glycine Proteins 0.000 description 1
- 108010015385 valyl-prolyl-proline Proteins 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
本发明涉及一种乳酸菌与嗜冷菌蛋白酶组合固态发酵制备火麻蛋白肽的方法，火麻粕通过粉碎、微生物接种、加酶混合、固态发酵、多肽提取制备、冷冻干燥等步骤获得火麻蛋白肽粉。本发明利用乳酸菌与嗜冷菌来源的蛋白酶协同进行固态发酵制备火麻蛋白多肽，一方面乳酸菌在固态发酵过程中能够产生蛋白酶、氨肽酶等多种酶类，有效水解火麻蛋白，且微生物的发酵作用能够降低单独酶解的苦味，并且能够产生胞外多糖等活性代谢产物；另一方面来源于嗜冷菌的蛋白酶的加入能够辅助进行蛋白降解，显著加快火麻蛋白的降解，提高火麻蛋白的降解效率。本发明提供的乳酸菌与嗜冷菌蛋白酶组合固态发酵制备火麻蛋白肽的方法，工艺简单，制备效率高，适宜工业化生产。
Description
技术领域
本发明属于微生物酶解发酵技术领域，尤其是涉及一种乳酸菌与嗜冷菌蛋白酶组合固态发酵制备火麻蛋白肽的方法。
背景技术
火麻仁别名***仁、麻仁，在国内有一千多年的食用历史，《本草纲目》中记载麻仁，甘、平、无毒。现代研究表明，火麻仁有较好的利尿、抗炎、降血压以及降血脂等生能力活性。火麻仁富含蛋白、油脂、膳食纤维、微量元素以及氨基酸等营养成分。火麻仁中蛋白质含量为25％，含有20种氨基酸，包括9种人体必需氨基酸。火麻仁蛋白中80％为麻仁球蛋白，20％约为白蛋白，是一种优良的植物蛋白，富含有精氨酸、谷氨酸、组氨酸等功能性氨基酸。火麻蛋白工业上主要从火麻籽榨油后的副产物火麻粕中获取，但是火麻蛋白结构紧密，尤其是加热后火麻蛋白因为变性导致溶解度下降，降低消化率，生物效价并不理想，需要寻找一种提高火麻蛋白生物利用效率的方法。
目前火麻蛋白主要通过酸碱水解或是酶解法获得火麻蛋白肽，酸碱水解条件较为剧烈，容易破坏水解多肽的构型以及活性，并且会产生有害副产物；酶水解法是目前火麻蛋白肽的主要制备方法，采用中性蛋白酶、木瓜蛋白酶等进行酶解，但是单纯的酶对火麻蛋白水解程度不高，酶解后的水解物中存在较多苦味肽，导致风味与口感不好。此外单纯酶解的成本较高，大规模工业化受到限制。
发明内容
本发明的目的就是为了提供一种乳酸菌与嗜冷菌蛋白酶组合固态发酵制备火麻蛋白肽的方法，以解决单纯的微生物固态发酵获得火麻蛋白肽效率较低的问题.
本发明提供的方法工艺简单，制备条件温和，适宜于大规模工业化生产。
本发明的目的可以通过以下技术方案来实现：
本发明提供一种乳酸菌与嗜冷菌蛋白酶组合固态发酵制备火麻蛋白肽的方法，利用具有产蛋白酶能力的乳酸菌与嗜冷菌蛋白酶协同进行火麻粕固态发酵，制备火麻蛋白肽。
在本发明的一个实施方式中，所述方法包括以下步骤：
(1)将压榨制油之后的火麻粕粉碎，得到火麻粕粉；
(2)将乳酸菌活化，制备乳酸菌发酵剂；
(3)将制备的乳酸菌发酵剂与嗜冷菌蛋白酶接入火麻粕粉中进行固态发酵；
(4)固态发酵结束后，将发酵火麻粕粉进行灭酶处理；
(5)将灭酶后的火麻粕粉进行热水浸提，离心去除沉淀，收集上清获得火麻蛋白肽粗提液；
(6)将火麻蛋白肽粗提液进行脱色、脱苦、分离纯化以及冷冻干燥获得火麻蛋白肽粉。
进一步的，在本发明的一个实施方式中，步骤(1)中，火麻粕粉的粒径为300-900μm。
进一步的，在本发明的一个实施方式中，步骤(1)中，火麻粕粉碎后经过121℃高温蒸汽灭菌处理。
进一步的，在本发明的一个实施方式中，步骤(2)中，乳酸菌发酵剂制备方法为：
a：配置培养基：蛋白胨10g/L，牛肉膏10g/L，酵母提取物5g/L，K2HPO4 2g/L，柠檬酸二铵2g/L，乙酸钠5g/L，葡萄糖20g/L，吐温80 1mL，MgSO4·7H2O 0.58g/L，MnSO4·4H2O0.25g/L；培养基配置好了后进行高温灭菌；
b：将乳酸菌活化后接种至培养基中，接种量为3％，培养20h后离心收集菌体，然后加入无菌水调节菌浓为1×109/mL，即为乳酸菌发酵剂。
进一步的，在本发明的一个实施方式中，步骤a中，进行高温灭菌的温度为121℃。
进一步的，在本发明的一个实施方式中，步骤(2)中，乳酸菌选择为唾液乳杆菌、植物乳杆菌、发酵乳杆菌或短乳杆菌中的一种或者几种。
进一步的，在本发明的一个实施方式中，步骤(3)中，加入无菌营养液至火麻粕粉中，控制火麻粕粉含水量在60％-85％(w/w)，无菌营养液中含有2％-15％的葡萄糖。
进一步的，在本发明的一个实施方式中，步骤(3)中，乳酸菌发酵剂接种量为5％-30％(v/w)；嗜冷菌蛋白酶添加量为30-220U/g，乳酸菌与嗜冷菌蛋白酶可以同步加入火麻粕粉中，也可以分步加入。
进一步的，在本发明的一个实施方式中，步骤(3)中，所述嗜冷菌蛋白酶为一种金属蛋白酶，其分离于荧光假单胞菌BNCC232070，为市售的成熟产品，菌株购买自商城北纳创联生物科技有限公司。其氨基酸序列如SEQ ID NO.1所示，具体如下：
MNGIPPSPTPLRLSAARVSQYEPEEETDAGKSVKMSLDTFQAGRIISRNGAVWGDQNRDGHTTIYYKFGQHNYTDQHDINTNFTESQKNGLRLAMQSWSDIANVAYKEKPPKGEWVDDGKSGKDTRDAEATLSLLKRVNSHDPGGYAGIGTSHVGITFRYAGESTLPNTEFAGVAIHELGHHLGLPHPGNYNGRGSDYEKDAAYNEDTIAHSIMSYFSEKNNGYDFKGSVVRTPMMDDISASQRIYGANYNTRNTDTTYGFNSNTGREALTFKSSRDRPVLCIWDGGGNDTLDCSKFAADQQINLNEQTFSNVAGLKGNISIARGVTLENAVGGHGNDQILGNDADNRLTGGVGADQLTGGKGKDTFIYKDISDSTPEKPDLITDFVSGTDSFDLSAMLKKNGLKDLHFVSQFSGRAGEALLDFDEDRNLGRLKVDLSGKGRENFMVTTNGRIYPSDVLSTPSLHKTVDTSPPRFTPDGARTDGYVPPPPPPPLVLPPTVKFMTGTQGNDVLRGDHGDNNITGGAGADVLFGGAGKDIFIYEKASDSSVKRPDQILDFTTGIDKIDVSKALQAAGTDTLIFVEKFTGRIGETVIRYNAKNKESSLAVDMTGNGKADLHIVSRGQIKADDIISK。
进一步的，在本发明的一个实施方式中，步骤(3)中，固态发酵温度为28-45℃，发酵时间为1-4天。
进一步的，在本发明的一个实施方式中，步骤(4)中，进行灭酶处理的条件为：100℃水浴灭酶20min。
进一步的，在本发明的一个实施方式中，步骤(5)中，将去离子水加入发酵火麻粕中进行搅拌提取，固液比为1:5-1:25(w/v)，提取温度为20-70℃，提取时间为40-240min，提取2次，合并提取液。
进一步的，在本发明的一个实施方式中，步骤(6)中，采用活性炭对火麻蛋白肽粗提液进行脱色、脱苦，采用超滤膜超滤制备不同分子量活性肽，超滤膜的截留分子量分别为10k道尔顿。
与现有技术相比，本发明提供的制备火麻蛋白肽的方法，能够直接以火麻粕为原料进行固态发酵，获得不同分子量的的火麻蛋白肽，生产工艺简单、原料成本低、生产效率高，容易实现大规模工业化生产。本发明采用乳酸菌作为发酵剂进行固态发酵，一方面乳酸菌作为兼性厌氧菌，适用于大批量固体物料的静置培养，另一方面乳酸菌能够合成蛋白酶、氨肽酶等多种酶类水解火麻蛋白，提高火麻蛋白的生物利用度。与酶解法相比，本发明提供的乳酸菌与嗜冷菌蛋白酶联合固态发酵方法，显著提高了活性肽制备效率，同时能够显著降低水解肽的苦味。
具体实施方式
下面结合具体实施例对本发明进行详细说明。本实施例以本发明技术方案为前提进行实施，给出了详细的实施方式和具体的操作过程，但本发明的保护范围不限于下述的实施例。
以下实施例中的唾液乳杆菌、植物乳杆菌、发酵乳杆菌或短乳杆菌均为本领域常规生物材料，以下实施例中嗜冷菌蛋白酶具体为一种金属蛋白酶，其分离于荧光假单胞菌BNCC232070，为市售的成熟产品，菌株购买自商城北纳创联生物科技有限公司。其氨基酸序列如SEQ ID NO.1所示。
实施例1：乳酸菌固态发酵火麻粕生产火麻蛋白肽(对照实验)
(1)将压榨制油之后的火麻粕粉碎，得到火麻粕粉；火麻粕粉的粒径为500μm，火麻粕粉碎后经过121℃高温蒸汽灭菌；
(2)将唾液乳杆菌活化，制备唾液乳杆菌发酵剂；
制备唾液乳杆菌发酵剂的步骤如下：
a：配置培养基：蛋白胨10g/L，牛肉膏10g/L，酵母提取物5g/L，K2HPO4 2g/L，柠檬酸二铵2g/L，乙酸钠5g/L，葡萄糖20g/L，吐温80 1mL，MgSO4·7H2O 0.58g/L，MnSO4·4H2O0.25g/L；培养基配置好了后进行121℃高温灭菌；
b：将唾液乳杆菌活化后接种至培养基中，接种量为3％，培养20h后离心收集菌体，然后加入无菌水调节菌浓为1×109/mL，即为唾液乳杆菌发酵剂；
(3)加入无菌营养液至火麻粕粉中，控制火麻粕粉含水量在75％(w/w)，无菌营养液中含有10％的葡萄糖；将制备的唾液乳杆菌发酵剂接入火麻粕粉中进行固态发酵；唾液乳杆菌发酵剂接种量为15％(v/w)；固态发酵温度为37℃，发酵时间为3天；
(4)固态发酵结束后，将发酵火麻粕粉进行100℃水浴灭酶20min；
(5)将去离子水加入灭酶后的火麻粕粉进行热水搅拌浸提，固液比为1:15(w/v)，提取温度为50℃，提取时间为150min，离心去除沉淀，提取2次，合并上清提取液，收集上清获得火麻蛋白肽粗提液；
(6)将火麻蛋白肽粗提液进行脱色、脱苦、分离纯化以及冷冻干燥获得火麻蛋白肽粉；采用活性炭对火麻蛋白肽粗提液进行脱色、脱苦，然后12000g高速离心去除杂质，采用截留分子量为10k道尔顿的超滤膜超滤制备多肽，截留后的多肽溶液经过冷冻干燥后得到具有抗氧化性的火麻蛋白肽粉，肽粉的蛋白质含量为80％(w/w)，分子量为1k道尔顿-5k道尔顿的多肽占57％，多肽的DPPH清除率达到66.3％。
实施例2：乳酸菌与嗜冷菌蛋白酶同步固态发酵火麻粕生产火麻蛋白肽
(1)将压榨制油之后的火麻粕粉碎，得到火麻粕粉；火麻粕粉的粒径为300μm，火麻粕粉碎后经过121℃高温蒸汽灭菌；
(2)将唾液乳杆菌活化，制备唾液乳杆菌发酵剂；
制备唾液乳杆菌发酵剂的步骤如下：
a：配置培养基：蛋白胨10g/L，牛肉膏10g/L，酵母提取物5g/L，K2HPO4 2g/L，柠檬酸二铵2g/L，乙酸钠5g/L，葡萄糖20g/L，吐温80 1mL，MgSO4·7H2O 0.58g/L，MnSO4·4H2O0.25g/L；培养基配置好了后进行121℃高温灭菌；
b：将唾液乳杆菌活化后接种至培养基中，接种量为3％，培养20h后离心收集菌体，然后加入无菌水调节菌浓为1×109/mL，即为唾液乳杆菌发酵剂；
(3)将制备的唾液乳杆菌发酵剂与嗜冷菌蛋白酶同步接入火麻粕粉中进行固态发酵；加入无菌营养液至火麻粕粉中，控制火麻粕粉含水量在60％(w/w)，无菌营养液中含有2％的葡萄糖；唾液乳杆菌发酵剂接种量为5％(v/w)，嗜冷菌蛋白酶添加量为30U/g，固态发酵温度为28℃，发酵时间为1天；
(4)固态发酵结束后，将发酵火麻粕粉进行100℃水浴灭酶20min；
(5)将去离子水加入灭酶后的火麻粕粉进行热水搅拌浸提，固液比为1:5(w/v)，提取温度为20℃，提取时间为40min，离心去除沉淀，提取2次，合并上清提取液，收集上清获得火麻蛋白肽粗提液；
(6)将火麻蛋白肽粗提液进行脱色、脱苦、分离纯化以及冷冻干燥获得火麻蛋白肽粉；采用活性炭对火麻蛋白肽粗提液进行脱色、脱苦，然后12000g高速离心去除杂质，采用截留分子量为10k道尔顿的超滤膜超滤制备多肽，截留后的多肽溶液经过冷冻干燥后得到具有抗氧化性的火麻蛋白肽粉，肽粉的蛋白质含量为83％(w/w)，分子量为1k道尔顿-5k道尔顿的多肽占61％，多肽的DPPH清除率达到78.5％。
实施例3：乳酸菌与嗜冷菌蛋白酶同步固态发酵火麻粕生产火麻蛋白肽
(1)将压榨制油之后的火麻粕粉碎，得到火麻粕粉；火麻粕粉的粒径为500μm，火麻粕粉碎后经过121℃高温蒸汽灭菌；
(2)将唾液乳杆菌活化，制备唾液乳杆菌发酵剂；
制备唾液乳杆菌发酵剂的步骤如下：
a：配置培养基：蛋白胨10g/L，牛肉膏10g/L，酵母提取物5g/L，K2HPO4 2g/L，柠檬酸二铵2g/L，乙酸钠5g/L，葡萄糖20g/L，吐温80 1mL，MgSO4·7H2O 0.58g/L，MnSO4·4H2O0.25g/L；培养基配置好了后进行121℃高温灭菌；
b：将唾液乳杆菌活化后接种至培养基中，接种量为3％，培养20h后离心收集菌体，然后加入无菌水调节菌浓为1×109/mL，即为唾液乳杆菌发酵剂；
(3)将制备的唾液乳杆菌发酵剂与嗜冷菌蛋白酶同步接入火麻粕粉中进行固态发酵；加入无菌营养液至火麻粕粉中，控制火麻粕粉含水量在75％(w/w)，无菌营养液中含有10％的葡萄糖；唾液乳杆菌发酵剂接种量为15％(v/w)，嗜冷菌蛋白酶添加量为150U/g，固态发酵温度为37℃，发酵时间为3天；
(4)固态发酵结束后，将发酵火麻粕粉进行100℃水浴灭酶20min；
(5)将去离子水加入灭酶后的火麻粕粉进行热水搅拌浸提，固液比为1:15(w/v)，提取温度为50℃，提取时间为150min，离心去除沉淀，提取2次，合并上清提取液，收集上清获得火麻蛋白肽粗提液；
(6)将火麻蛋白肽粗提液进行脱色、脱苦、分离纯化以及冷冻干燥获得火麻蛋白肽粉；采用活性炭对火麻蛋白肽粗提液进行脱色、脱苦，然后12000g高速离心去除杂质，采用截留分子量为10k道尔顿的超滤膜超滤制备多肽，截留后的多肽溶液经过冷冻干燥后得到具有抗氧化性的火麻蛋白肽粉，肽粉的蛋白质含量为90％(w/w)，分子量为1k道尔顿-5k道尔顿的多肽占71％，多肽的DPPH清除率达到94.9％。
实施例4：乳酸菌与嗜冷菌蛋白酶同步固态发酵火麻粕生产火麻蛋白肽
(1)将压榨制油之后的火麻粕粉碎，得到火麻粕粉；火麻粕粉的粒径为500μm，火麻粕粉碎后经过121℃高温蒸汽灭菌；
(2)将植物乳杆菌活化，制备植物乳杆菌发酵剂；
制备植物乳杆菌发酵剂的步骤如下：
a：配置培养基：蛋白胨10g/L，牛肉膏10g/L，酵母提取物5g/L，K2HPO4 2g/L，柠檬酸二铵2g/L，乙酸钠5g/L，葡萄糖20g/L，吐温80 1mL，MgSO4·7H2O 0.58g/L，MnSO4·4H2O0.25g/L；培养基配置好了后进行121℃高温灭菌；
b：将植物乳杆菌活化后接种至培养基中，接种量为3％，培养20h后离心收集菌体，然后加入无菌水调节菌浓为1×109/mL，即为植物乳杆菌发酵剂；
(3)将制备的植物乳杆菌发酵剂与嗜冷菌蛋白酶同步接入火麻粕粉中进行固态发酵；加入无菌营养液至火麻粕粉中，控制火麻粕粉含水量在75％(w/w)，无菌营养液中含有10％的葡萄糖；植物乳杆菌发酵剂接种量为15％(v/w)，嗜冷菌蛋白酶添加量为150U/g，固态发酵温度为37℃，发酵时间为3天；
(4)固态发酵结束后，将发酵火麻粕粉进行100℃水浴灭酶20min；
(5)将去离子水加入灭酶后的火麻粕粉进行热水搅拌浸提，固液比为1:15(w/v)，提取温度为50℃，提取时间为150min，离心去除沉淀，提取2次，合并上清提取液，收集上清获得火麻蛋白肽粗提液；
(6)将火麻蛋白肽粗提液进行脱色、脱苦、分离纯化以及冷冻干燥获得火麻蛋白肽粉；采用活性炭对火麻蛋白肽粗提液进行脱色、脱苦，然后12000g高速离心去除杂质，采用截留分子量为10k道尔顿的超滤膜超滤制备多肽，截留后的多肽溶液经过冷冻干燥后得到具有抗氧化性的火麻蛋白肽粉，肽粉的蛋白质含量为85％(w/w)，分子量为1k道尔顿-5k道尔顿的多肽占65％，多肽的DPPH清除率达到91.2％。
实施例5：乳酸菌与嗜冷菌蛋白酶同步固态发酵火麻粕生产火麻蛋白肽
(1)将压榨制油之后的火麻粕粉碎，得到火麻粕粉；火麻粕粉的粒径为900μm，火麻粕粉碎后经过121℃高温蒸汽灭菌；
(2)将唾液乳杆菌活化，制备唾液乳杆菌发酵剂；
制备唾液乳杆菌发酵剂的步骤如下：
a：配置培养基：蛋白胨10g/L，牛肉膏10g/L，酵母提取物5g/L，K2HPO4 2g/L，柠檬酸二铵2g/L，乙酸钠5g/L，葡萄糖20g/L，吐温80 1mL，MgSO4·7H2O 0.58g/L，MnSO4·4H2O0.25g/L；培养基配置好了后进行121℃高温灭菌；
b：将唾液乳杆菌活化后接种至培养基中，接种量为3％，培养20h后离心收集菌体，然后加入无菌水调节菌浓为1×109/mL，即为唾液乳杆菌发酵剂；
(3)将制备的唾液乳杆菌发酵剂与嗜冷菌蛋白酶同步接入火麻粕粉中进行固态发酵；加入无菌营养液至火麻粕粉中，控制火麻粕粉含水量在85％(w/w)，无菌营养液中含有15％的葡萄糖；唾液乳杆菌发酵剂接种量为30％(v/w)，嗜冷菌蛋白酶添加量为220U/g，固态发酵温度为45℃，发酵时间为4天；
(4)固态发酵结束后，将发酵火麻粕粉进行100℃水浴灭酶20min；
(5)将去离子水加入灭酶后的火麻粕粉进行热水搅拌浸提，固液比为1:25(w/v)，提取温度为70℃，提取时间为240min，离心去除沉淀，提取2次，合并上清提取液，收集上清获得火麻蛋白肽粗提液；
(6)将火麻蛋白肽粗提液进行脱色、脱苦、分离纯化以及冷冻干燥获得火麻蛋白肽粉；采用活性炭对火麻蛋白肽粗提液进行脱色、脱苦，然后12000g高速离心去除杂质，采用截留分子量为10k道尔顿的超滤膜超滤制备多肽，截留后的多肽溶液经过冷冻干燥后得到具有抗氧化性的火麻蛋白肽粉，肽粉的蛋白质含量为85％(w/w)，分子量为1k道尔顿-5k道尔顿的多肽占62％，多肽的DPPH清除率达到82.2％。
实施例6：乳酸菌与嗜冷菌蛋白酶分步固态发酵火麻粕生产火麻蛋白肽
(1)将压榨制油之后的火麻粕粉碎，得到火麻粕粉；火麻粕粉的粒径为500μm，火麻粕粉碎后经过121℃高温蒸汽灭菌；
(2)将唾液乳杆菌活化，制备唾液乳杆菌发酵剂；
制备唾液乳杆菌发酵剂的步骤如下：
a：配置培养基：蛋白胨10g/L，牛肉膏10g/L，酵母提取物5g/L，K2HPO4 2g/L，柠檬酸二铵2g/L，乙酸钠5g/L，葡萄糖20g/L，吐温80 1mL，MgSO4·7H2O 0.58g/L，MnSO4·4H2O0.25g/L；培养基配置好了后进行121℃高温灭菌；
b：将唾液乳杆菌活化后接种至培养基中，接种量为3％，培养20h后离心收集菌体，然后加入无菌水调节菌浓为1×109/mL，即为唾液乳杆菌发酵剂；
(3)将制备的唾液乳杆菌发酵剂与嗜冷菌蛋白酶分步接入火麻粕粉中进行固态发酵；加入无菌营养液至火麻粕粉中，控制火麻粕粉含水量在85％(w/w)，无菌营养液中含有15％的葡萄糖；唾液乳杆菌发酵剂接种量为15％(v/w)，发酵温度为37℃，发酵1天后，按150U/g加入嗜冷菌蛋白酶，搅拌均匀后相同条件下继续发酵2天；
(4)固态发酵结束后，将发酵火麻粕粉进行100℃水浴灭酶20min；
(5)将去离子水加入灭酶后的火麻粕粉进行热水搅拌浸提，固液比为1:25(w/v)，提取温度为70℃，提取时间为240min，离心去除沉淀，提取2次，合并上清提取液，收集上清获得火麻蛋白肽粗提液；
(6)将火麻蛋白肽粗提液进行脱色、脱苦、分离纯化以及冷冻干燥获得火麻蛋白肽粉；采用活性炭对火麻蛋白肽粗提液进行脱色、脱苦，然后12000g高速离心去除杂质，采用截留分子量为10k道尔顿的超滤膜超滤制备多肽，截留后的多肽溶液经过冷冻干燥后得到具有抗氧化性的火麻蛋白肽粉，肽粉的蛋白质含量为86％(w/w)，分子量为1k道尔顿-5k道尔顿的多肽占67％，多肽的DPPH清除率达到89.8％。
实施例7：乳酸菌与嗜冷菌蛋白酶分步固态发酵火麻粕生产火麻蛋白肽
(1)将压榨制油之后的火麻粕粉碎，得到火麻粕粉；火麻粕粉的粒径为500μm，火麻粕粉碎后经过121℃高温蒸汽灭菌；
(2)将植物乳杆菌活化，制备植物乳杆菌发酵剂；
制备植物乳杆菌发酵剂的步骤如下：
a：配置培养基：蛋白胨10g/L，牛肉膏10g/L，酵母提取物5g/L，K2HPO4 2g/L，柠檬酸二铵2g/L，乙酸钠5g/L，葡萄糖20g/L，吐温80 1mL，MgSO4·7H2O 0.58g/L，MnSO4·4H2O0.25g/L；培养基配置好了后进行121℃高温灭菌；
b：将植物乳杆菌活化后接种至培养基中，接种量为3％，培养20h后离心收集菌体，然后加入无菌水调节菌浓为1×109/mL，即为植物乳杆菌发酵剂；
(3)将制备的植物乳杆菌发酵剂与嗜冷菌蛋白酶分步接入火麻粕粉中进行固态发酵；加入无菌营养液至火麻粕粉中，控制火麻粕粉含水量在85％(w/w)，无菌营养液中含有15％的葡萄糖；植物乳杆菌发酵剂接种量为15％(v/w)，发酵温度为37℃，发酵1天后，按150U/g加入嗜冷菌蛋白酶，搅拌均匀后相同条件下继续发酵2天；
(4)固态发酵结束后，将发酵火麻粕粉进行100℃水浴灭酶20min；
(5)将去离子水加入灭酶后的火麻粕粉进行热水搅拌浸提，固液比为1:25(w/v)，提取温度为70℃，提取时间为240min，离心去除沉淀，提取2次，合并上清提取液，收集上清获得火麻蛋白肽粗提液；
(6)将火麻蛋白肽粗提液进行脱色、脱苦、分离纯化以及冷冻干燥获得火麻蛋白肽粉；采用活性炭对火麻蛋白肽粗提液进行脱色、脱苦，然后12000g高速离心去除杂质，采用截留分子量为10k道尔顿的超滤膜超滤制备多肽，截留后的多肽溶液经过冷冻干燥后得到具有抗氧化性的火麻蛋白肽粉，肽粉的蛋白质含量为82％(w/w)，分子量为1k道尔顿-5k道尔顿的多肽占63％，多肽的DPPH清除率达到87.5％。
下表是本发明的实施例与对照例的实验效果对比
综上所述，本发明采用乳酸菌作为发酵剂进行固态发酵，一方面乳酸菌作为兼性厌氧菌，适用于大批量固体物料的静置培养，另一方面乳酸菌能够合成蛋白酶、氨肽酶等多种酶类水解火麻蛋白，提高火麻蛋白的生物利用度。与酶解法相比，本专利提供的乳酸菌与嗜冷菌蛋白酶联合固态发酵方法，显著提高了活性肽制备效率，同时能够显著降低水解肽的苦味。本发明提供的制备火麻蛋白肽的方法，能够直接以火麻粕为原料进行固态发酵，获得不同分子量的火麻蛋白肽，生产工艺简单、原料成本低、生产效率高，容易实现大规模工业化生产。
上述的对实施例的描述是为便于该技术领域的普通技术人员能理解和使用发明。熟悉本领域技术的人员显然可以容易地对这些实施例做出各种修改，并把在此说明的一般原理应用到其他实施例中而不必经过创造性的劳动。因此，本发明不限于上述实施例，本领域技术人员根据本发明的揭示，不脱离本发明范畴所做出的改进和修改都应该在本发明的保护范围之内。
序列表
<110> 上海理工大学
<120> 一种乳酸菌与嗜冷菌蛋白酶组合固态发酵制备火麻蛋白肽的方法
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 633
<212> PRT
<213> 荧光假单胞菌(Pseudomonas fluorescens )
<400> 1
Met Asn Gly Ile Pro Pro Ser Pro Thr Pro Leu Arg Leu Ser Ala Ala
1 5 10 15
Arg Val Ser Gln Tyr Glu Pro Glu Glu Glu Thr Asp Ala Gly Lys Ser
20 25 30
Val Lys Met Ser Leu Asp Thr Phe Gln Ala Gly Arg Ile Ile Ser Arg
35 40 45
Asn Gly Ala Val Trp Gly Asp Gln Asn Arg Asp Gly His Thr Thr Ile
50 55 60
Tyr Tyr Lys Phe Gly Gln His Asn Tyr Thr Asp Gln His Asp Ile Asn
65 70 75 80
Thr Asn Phe Thr Glu Ser Gln Lys Asn Gly Leu Arg Leu Ala Met Gln
85 90 95
Ser Trp Ser Asp Ile Ala Asn Val Ala Tyr Lys Glu Lys Pro Pro Lys
100 105 110
Gly Glu Trp Val Asp Asp Gly Lys Ser Gly Lys Asp Thr Arg Asp Ala
115 120 125
Glu Ala Thr Leu Ser Leu Leu Lys Arg Val Asn Ser His Asp Pro Gly
130 135 140
Gly Tyr Ala Gly Ile Gly Thr Ser His Val Gly Ile Thr Phe Arg Tyr
145 150 155 160
Ala Gly Glu Ser Thr Leu Pro Asn Thr Glu Phe Ala Gly Val Ala Ile
165 170 175
His Glu Leu Gly His His Leu Gly Leu Pro His Pro Gly Asn Tyr Asn
180 185 190
Gly Arg Gly Ser Asp Tyr Glu Lys Asp Ala Ala Tyr Asn Glu Asp Thr
195 200 205
Ile Ala His Ser Ile Met Ser Tyr Phe Ser Glu Lys Asn Asn Gly Tyr
210 215 220
Asp Phe Lys Gly Ser Val Val Arg Thr Pro Met Met Asp Asp Ile Ser
225 230 235 240
Ala Ser Gln Arg Ile Tyr Gly Ala Asn Tyr Asn Thr Arg Asn Thr Asp
245 250 255
Thr Thr Tyr Gly Phe Asn Ser Asn Thr Gly Arg Glu Ala Leu Thr Phe
260 265 270
Lys Ser Ser Arg Asp Arg Pro Val Leu Cys Ile Trp Asp Gly Gly Gly
275 280 285
Asn Asp Thr Leu Asp Cys Ser Lys Phe Ala Ala Asp Gln Gln Ile Asn
290 295 300
Leu Asn Glu Gln Thr Phe Ser Asn Val Ala Gly Leu Lys Gly Asn Ile
305 310 315 320
Ser Ile Ala Arg Gly Val Thr Leu Glu Asn Ala Val Gly Gly His Gly
325 330 335
Asn Asp Gln Ile Leu Gly Asn Asp Ala Asp Asn Arg Leu Thr Gly Gly
340 345 350
Val Gly Ala Asp Gln Leu Thr Gly Gly Lys Gly Lys Asp Thr Phe Ile
355 360 365
Tyr Lys Asp Ile Ser Asp Ser Thr Pro Glu Lys Pro Asp Leu Ile Thr
370 375 380
Asp Phe Val Ser Gly Thr Asp Ser Phe Asp Leu Ser Ala Met Leu Lys
385 390 395 400
Lys Asn Gly Leu Lys Asp Leu His Phe Val Ser Gln Phe Ser Gly Arg
405 410 415
Ala Gly Glu Ala Leu Leu Asp Phe Asp Glu Asp Arg Asn Leu Gly Arg
420 425 430
Leu Lys Val Asp Leu Ser Gly Lys Gly Arg Glu Asn Phe Met Val Thr
435 440 445
Thr Asn Gly Arg Ile Tyr Pro Ser Asp Val Leu Ser Thr Pro Ser Leu
450 455 460
His Lys Thr Val Asp Thr Ser Pro Pro Arg Phe Thr Pro Asp Gly Ala
465 470 475 480
Arg Thr Asp Gly Tyr Val Pro Pro Pro Pro Pro Pro Pro Leu Val Leu
485 490 495
Pro Pro Thr Val Lys Phe Met Thr Gly Thr Gln Gly Asn Asp Val Leu
500 505 510
Arg Gly Asp His Gly Asp Asn Asn Ile Thr Gly Gly Ala Gly Ala Asp
515 520 525
Val Leu Phe Gly Gly Ala Gly Lys Asp Ile Phe Ile Tyr Glu Lys Ala
530 535 540
Ser Asp Ser Ser Val Lys Arg Pro Asp Gln Ile Leu Asp Phe Thr Thr
545 550 555 560
Gly Ile Asp Lys Ile Asp Val Ser Lys Ala Leu Gln Ala Ala Gly Thr
565 570 575
Asp Thr Leu Ile Phe Val Glu Lys Phe Thr Gly Arg Ile Gly Glu Thr
580 585 590
Val Ile Arg Tyr Asn Ala Lys Asn Lys Glu Ser Ser Leu Ala Val Asp
595 600 605
Met Thr Gly Asn Gly Lys Ala Asp Leu His Ile Val Ser Arg Gly Gln
610 615 620
Ile Lys Ala Asp Asp Ile Ile Ser Lys
625 630
Claims (1)
1.一种乳酸菌与嗜冷菌蛋白酶组合固态发酵制备火麻蛋白肽的方法，其特征在于，利用具有产蛋白酶能力的乳酸菌与嗜冷菌蛋白酶协同进行火麻粕固态发酵，制备火麻蛋白肽，所述方法包括以下步骤：
（1）将压榨制油之后的火麻粕粉碎，得到火麻粕粉；
（2）将乳酸菌活化，制备乳酸菌发酵剂；
（3）将制备的乳酸菌发酵剂与嗜冷菌蛋白酶接入火麻粕粉中进行固态发酵；
（4）固态发酵结束后，将发酵火麻粕粉进行灭酶处理；
（5）将灭酶后的火麻粕粉进行热水浸提，离心去除沉淀，收集上清获得火麻蛋白肽粗提液；
（6）将火麻蛋白肽粗提液进行脱色、脱苦、分离纯化以及冷冻干燥获得火麻蛋白肽粉；
步骤（2）中，乳酸菌发酵剂制备方法为：
a：配置培养基：蛋白胨10g/L，牛肉膏10g/L，酵母提取物5g/L，K2HPO4 2g/L，柠檬酸二铵2g/L，乙酸钠5g/L，葡萄糖20g/L，吐温80 1mL，MgSO4•7H2O 0.58g/L，MnSO4•4H2O 0.25g/L；培养基配置好了后进行高温灭菌；
b：将乳酸菌活化后接种至培养基中，接种量为3%，培养后离心收集菌体，然后加入无菌水调节菌浓，即为乳酸菌发酵剂；
步骤（2）中，乳酸菌选择为唾液乳杆菌或植物乳杆菌；
步骤（3）中，所述嗜冷菌蛋白酶的氨基酸序列如SEQ ID NO.1所示；
步骤（1）中，火麻粕粉的粒径为300-900 μm，步骤（1）中，火麻粕粉碎后经过高温蒸汽灭菌处理；
步骤（3）中，加入无菌营养液至火麻粕粉中，控制火麻粕粉含水量在75%-85%（w/w），无菌营养液中含有10%-15%的葡萄糖；步骤（3）中，乳酸菌发酵剂接种量为15%（v/w）；嗜冷菌蛋白酶添加量为150 U/g；乳酸菌与嗜冷菌蛋白酶同步加入火麻粕粉中，或分步加入；步骤（3）中，固态发酵温度为37-45℃，发酵时间为3-4天；
步骤（5）中，将去离子水加入发酵火麻粕中进行搅拌提取，固液比为1:15-1:25（w/v），提取温度为50-70℃，提取时间为150-240 min，提取2次，合并提取液；
步骤（6）中，采用活性炭对火麻蛋白肽粗提液进行脱色、脱苦，采用超滤膜超滤制备不同分子量活性肽，超滤膜的截留分子量为10 k道尔顿。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210132689.8A CN114921513B (zh) | 2022-02-14 | 2022-02-14 | 一种乳酸菌与嗜冷菌蛋白酶组合固态发酵制备火麻蛋白肽的方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210132689.8A CN114921513B (zh) | 2022-02-14 | 2022-02-14 | 一种乳酸菌与嗜冷菌蛋白酶组合固态发酵制备火麻蛋白肽的方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114921513A CN114921513A (zh) | 2022-08-19 |
| CN114921513B true CN114921513B (zh) | 2024-05-31 |
Family
ID=82804475
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210132689.8A Active CN114921513B (zh) | 2022-02-14 | 2022-02-14 | 一种乳酸菌与嗜冷菌蛋白酶组合固态发酵制备火麻蛋白肽的方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114921513B (zh) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2013188198A (ja) * | 2012-02-16 | 2013-09-26 | Kanazawa Univ | 免疫抑制乳酸菌組成物及び免疫抑制乳酸発酵食品 |
| CN103911420A (zh) * | 2014-04-08 | 2014-07-09 | 南京财经大学 | 菌酶协同发酵菜籽粕制备菜籽肽的方法 |
| CN106754840A (zh) * | 2016-12-22 | 2017-05-31 | 江南大学 | 一种酶活及热稳定性改善的凝乳酶突变体 |
| CN109402095A (zh) * | 2018-10-19 | 2019-03-01 | 湖南汇升生物科技有限公司 | 一种蛋白酶及其制备低镉大米多肽的方法 |
| CN110511897A (zh) * | 2019-09-16 | 2019-11-29 | 江南大学 | 一种沙雷氏菌及其在蛋白酶生产中的应用 |
| CN112410393A (zh) * | 2020-11-30 | 2021-02-26 | 中国农业科学院麻类研究所 | 一种火麻活性肽及其制备方法和应用 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090299036A1 (en) * | 2006-02-14 | 2009-12-03 | Calpis Co, Ltd | Agent for preventing arteriosclerosis, agent for suppressing vascular intimal thickening and agent for improving vascular endothelial function |
| US10716314B2 (en) * | 2015-12-16 | 2020-07-21 | Godo Shusei Co., Ltd. | Yogurt product and method for manufacturing same |
| US20210045419A1 (en) * | 2018-02-20 | 2021-02-18 | University Of Manitoba | Food Protein-Derived Peptides as Bitter Taste Blockers |
-
2022
- 2022-02-14 CN CN202210132689.8A patent/CN114921513B/zh active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2013188198A (ja) * | 2012-02-16 | 2013-09-26 | Kanazawa Univ | 免疫抑制乳酸菌組成物及び免疫抑制乳酸発酵食品 |
| CN103911420A (zh) * | 2014-04-08 | 2014-07-09 | 南京财经大学 | 菌酶协同发酵菜籽粕制备菜籽肽的方法 |
| CN106754840A (zh) * | 2016-12-22 | 2017-05-31 | 江南大学 | 一种酶活及热稳定性改善的凝乳酶突变体 |
| CN109402095A (zh) * | 2018-10-19 | 2019-03-01 | 湖南汇升生物科技有限公司 | 一种蛋白酶及其制备低镉大米多肽的方法 |
| CN110511897A (zh) * | 2019-09-16 | 2019-11-29 | 江南大学 | 一种沙雷氏菌及其在蛋白酶生产中的应用 |
| CN112410393A (zh) * | 2020-11-30 | 2021-02-26 | 中国农业科学院麻类研究所 | 一种火麻活性肽及其制备方法和应用 |
Non-Patent Citations (2)
| Title |
|---|
| MULTISPECIES: M10 family metallopeptidase C-terminal domain-containing protein [Pseudomonas fluorescens group];Accession No:WP_080889138.1;Genbank Database;第1页 * |
| 麻仁粕液体发酵的研究;林金莺等;中国调味品;第34卷(第03期);第39-42页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114921513A (zh) | 2022-08-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109251954B (zh) | 一种海参多肽的生产方法 | |
| CN112999127B (zh) | 一种龙胆复方酵素及其制备方法和应用 | |
| CN106916861B (zh) | 一种同时生产木耳多糖和黑色素的方法 | |
| CN107937311B (zh) | 一株高产γ-氨基丁酸的嗜热链球菌、保存培养方法及利用该嗜热链球菌制备发酵乳的方法 | |
| CN113966832A (zh) | 一种石斛发酵物及其制备方法和应用 | |
| CN102660622A (zh) | 一种混菌固态发酵制备大豆活性肽的方法 | |
| CN103911420A (zh) | 菌酶协同发酵菜籽粕制备菜籽肽的方法 | |
| CN101434981B (zh) | 一种微生物固态发酵制备生物活性专一的菜籽肽的方法 | |
| CN101434982B (zh) | 一种微生物固态发酵制备菜籽活性肽的方法 | |
| CN101654697B (zh) | 一种混菌发酵制备菜籽肽的方法 | |
| CN104152522A (zh) | 一种混菌固态发酵谷朊粉制备小麦活性肽的方法 | |
| CN108203729B (zh) | 一种巨藻抗氧化肽的制备方法 | |
| CN114921513B (zh) | 一种乳酸菌与嗜冷菌蛋白酶组合固态发酵制备火麻蛋白肽的方法 | |
| CN111349678A (zh) | 一种油菜花粉多糖提取方法以及提取产物 | |
| CN101654693B (zh) | 微生物发酵制备菜籽肽的方法 | |
| CN101492708B (zh) | 混菌固态发酵制备生物活性专一的菜籽肽的方法 | |
| CN101440390B (zh) | 混菌固态发酵制备菜籽活性肽的方法 | |
| CN101654696B (zh) | 一种微生物液态发酵制备菜籽肽的方法 | |
| CN111345419A (zh) | 一种复合发酵型鲨鱼软骨粉保健饮品 | |
| CN111329071B (zh) | 一种脑多肽的制备工艺 | |
| KR20160121758A (ko) | 유자씨 유래 비당체 플라보노이드 함유 추출물의 제조방법 및 이를 함유하는 화장료 조성물 | |
| CN113801908B (zh) | 一种乳清蛋白源缓解体力疲劳功能肽及其制备方法 | |
| CN112899330A (zh) | 一种火麻仁蛋白制备抗氧化肽的方法 | |
| CN109355335B (zh) | 芹菜籽发酵制备的降血脂肽的提取方法 | |
| CN115089529B (zh) | 一种具有祛痘功效的绿豆皮发酵产物及其制备方法和应用 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |