CN114847077A - Method for treating yeast industrial wastewater by using edible fungus production technology - Google Patents
Method for treating yeast industrial wastewater by using edible fungus production technology Download PDFInfo
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- 241000233866 Fungi Species 0.000 title claims abstract description 114
- 240000004808 Saccharomyces cerevisiae Species 0.000 title claims abstract description 98
- 239000010842 industrial wastewater Substances 0.000 title claims abstract description 82
- 238000000034 method Methods 0.000 title claims abstract description 44
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 39
- 238000005516 engineering process Methods 0.000 title claims abstract description 34
- 239000000463 material Substances 0.000 claims abstract description 92
- 239000002994 raw material Substances 0.000 claims abstract description 23
- 229910052602 gypsum Inorganic materials 0.000 claims abstract description 19
- 239000010440 gypsum Substances 0.000 claims abstract description 19
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 18
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 18
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 18
- 235000009566 rice Nutrition 0.000 claims abstract description 18
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 18
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 17
- 229930006000 Sucrose Natural products 0.000 claims abstract description 17
- 235000012343 cottonseed oil Nutrition 0.000 claims abstract description 17
- 240000000249 Morus alba Species 0.000 claims abstract description 15
- 235000008708 Morus alba Nutrition 0.000 claims abstract description 15
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims abstract description 14
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims abstract description 14
- 238000002156 mixing Methods 0.000 claims abstract description 11
- 239000005720 sucrose Substances 0.000 claims abstract description 4
- 239000002023 wood Substances 0.000 claims abstract description 3
- 240000007594 Oryza sativa Species 0.000 claims abstract 5
- 229960004793 sucrose Drugs 0.000 claims description 16
- 240000001462 Pleurotus ostreatus Species 0.000 claims description 13
- 235000001603 Pleurotus ostreatus Nutrition 0.000 claims description 13
- 239000000203 mixture Substances 0.000 claims description 10
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 claims description 8
- 230000001954 sterilising effect Effects 0.000 claims description 7
- 239000002361 compost Substances 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 5
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- 239000008399 tap water Substances 0.000 claims description 3
- 235000020679 tap water Nutrition 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 230000008569 process Effects 0.000 claims description 2
- 240000000599 Lentinula edodes Species 0.000 claims 1
- 241000222350 Pleurotus Species 0.000 claims 1
- 241000392443 Pleurotus citrinopileatus Species 0.000 claims 1
- 244000252132 Pleurotus eryngii Species 0.000 claims 1
- 235000001681 Pleurotus eryngii Nutrition 0.000 claims 1
- 230000008901 benefit Effects 0.000 abstract description 12
- 230000006872 improvement Effects 0.000 abstract description 8
- 241000209094 Oryza Species 0.000 description 13
- 230000000052 comparative effect Effects 0.000 description 10
- 238000011081 inoculation Methods 0.000 description 8
- 239000002351 wastewater Substances 0.000 description 8
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 150000004676 glycans Chemical class 0.000 description 4
- 238000003306 harvesting Methods 0.000 description 4
- 229920001282 polysaccharide Polymers 0.000 description 4
- 239000005017 polysaccharide Substances 0.000 description 4
- 150000001413 amino acids Chemical class 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 239000002366 mineral element Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 238000004065 wastewater treatment Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 240000001624 Espostoa lanata Species 0.000 description 1
- 235000009161 Espostoa lanata Nutrition 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- NKLPQNGYXWVELD-UHFFFAOYSA-M coomassie brilliant blue Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=C1 NKLPQNGYXWVELD-UHFFFAOYSA-M 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 206010013781 dry mouth Diseases 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000021552 granulated sugar Nutrition 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 238000006396 nitration reaction Methods 0.000 description 1
- 235000021049 nutrient content Nutrition 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000036284 oxygen consumption Effects 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses a method for treating yeast industrial wastewater by using an edible fungus production technology, which comprises the following steps of mixing the following raw materials to prepare an edible fungus culture material; the raw materials comprise: yeast industrial wastewater, gypsum, monopotassium phosphate and sucrose; and at least one of wood chips, corncobs and mulberry twigs; and at least one of wheat bran, rice bran and cottonseed hull; wherein the mass percentage concentration of the yeast industrial wastewater in the edible fungus culture material is 5-40%. According to the method for treating the yeast industrial wastewater by using the edible fungus production technology, the yeast industrial wastewater is prepared into the culture material of the edible fungus for cultivation of the edible fungus, so that the yeast industrial wastewater is treated by using an edible fungus production system, and the problem of saving the treatment cost of the yeast industrial wastewater is solved; meanwhile, secondary economic benefits are generated by cultivating the edible fungi, and the method has important promotion and practical value on quality improvement and efficiency improvement of the yeast industry.
Description
Technical Field
The invention belongs to the technical field of wastewater treatment, and particularly relates to a method for treating yeast industrial wastewater by using an edible fungus production technology.
Background
The yeast industrial wastewater is high-concentration, high-organic-matter and high-pigment-content organic wastewater which is generated mainly in a separation process in a yeast production process and is difficult to treat, the wastewater contains abundant residual sugar, residual nitrogen and residual phosphorus which are not utilized by yeast, the total COD and BOD are high, and pigment which is brought by molasses and is difficult to degrade and treat is also contained, in order to reach a discharge standard, the yeast industrial wastewater needs to be treated by strict anaerobic treatment, oxygen consumption and the like, and not only the treatment steps are complex, but also the cost is high. Secondly, the amount of wastewater generated by the yeast industry is large, and the average production of 1 ton of dry yeast will generate about 20 cubic meters of wastewater, so the treatment of the yeast wastewater becomes one of the bottlenecks restricting the development of the yeast industry, and the research on the high-value utilization of the yeast wastewater has important significance for the development of the yeast industry.
The edible fungi is a food rich in various vitamin mineral elements and dietary fibers, is high in protein and low in fat, is delicious in taste, is increasingly favored by people, has a very good market prospect, and can be widely cultivated in China and even all over the world. At present, agricultural and forestry wastes are mainly used as culture materials for edible fungi cultivation, the edible fungi cultivation method has wide growth adaptability, and carbon sources, nitrogen sources and mineral elements contained in yeast wastewater are nutrient sources required by the growth of the edible fungi. However, at present, no method for treating yeast industrial wastewater by using an edible fungus production technology exists.
Disclosure of Invention
The object of the present invention is to solve at least the above drawbacks and to provide advantages which will be explained later.
The invention also aims to provide a method for treating the yeast industrial wastewater by using the edible fungus production technology, which solves the problem of saving the treatment cost of the yeast industrial wastewater by using an edible fungus production system to treat the yeast industrial wastewater by preparing the yeast industrial wastewater into a culture material of edible fungi for cultivating the edible fungi.
The invention also aims to provide a method for treating the yeast industrial wastewater by using the edible fungus production technology, which generates secondary economic benefit by cultivating the edible fungi and has important promotion and practical value on quality improvement and efficiency improvement of the yeast industry.
In order to achieve these objects and other advantages of the present invention, there is provided a method for treating yeast industrial wastewater using edible fungi production technology, comprising mixing the following raw materials to prepare an edible fungi culture material; the raw materials comprise: yeast industrial wastewater, gypsum, monopotassium phosphate and sucrose; and at least one of wood chips, corncobs and mulberry twigs; and at least one of wheat bran, rice bran and cottonseed hull; wherein the mass percentage concentration of the yeast industrial wastewater in the edible fungus culture material is 5-40%.
In the scheme, firstly, the yeast industrial wastewater is mixed with other raw materials to prepare the edible fungus culture material in the method for treating the yeast industrial wastewater by utilizing the edible fungus production technology, so that the yeast industrial wastewater is subjected to zero-cost and harmless treatment, and the cost for specially treating the yeast industrial wastewater is saved for the yeast industry.
Secondly, in the method for treating the yeast industrial wastewater by utilizing the edible fungus production technology, the yeast industrial wastewater is mixed with other raw materials to prepare the edible fungus culture material for cultivating the edible fungi, so that secondary economic benefits are generated, and the method has important promotion and practical value on quality improvement and efficiency improvement of the yeast industry.
Preferably, the mass percentage concentration of the yeast industrial wastewater in the edible fungus culture material is 10-20%.
Preferably, the edible fungus culture material comprises the following raw materials in parts by weight: 10-20 parts of yeast industrial wastewater, 60-80 parts of sawdust, 60-70 parts of corncobs, 10-15 parts of wheat bran, 20-25 parts of rice bran, 10-20 parts of cottonseed hulls, 5-20 parts of mulberry twigs, 0-1 part of cane sugar, 0.5-1.5 parts of gypsum and 0.2-0.5 part of potassium dihydrogen phosphate.
Preferably, the edible fungus culture medium specifically comprises the following raw materials in parts by weight: 10 parts of yeast industrial wastewater, 60 parts of sawdust, 10 parts of corncobs, 8 parts of wheat bran, 2 parts of rice bran, 5 parts of cottonseed hulls, 5 parts of mulberry twigs, 1 part of cane sugar, 1 part of gypsum and 0.2 part of monopotassium phosphate.
In the scheme, the method for treating the yeast industrial wastewater by using the edible fungus production technology disclosed by the invention has the advantages that the raw materials are mixed according to the specific weight ratio to prepare the edible fungus culture material, so that the yeast industrial wastewater can be treated in time, and the proportioned edible fungus culture material can meet the growth requirement of edible fungi, so that secondary economic benefits are generated.
Preferably, the pH value of the edible fungus culture material is 5.5-7.5.
Preferably, the edible fungus culture material can be used for cultivating oyster mushrooms.
In the scheme, the method for treating the yeast industrial wastewater by using the edible fungus production technology disclosed by the invention is used for preparing the edible fungus culture material by mixing the yeast industrial wastewater with other materials, and the obtained edible fungus culture material is wide in application range, can be applied to cultivation of various edible fungi, and has a good market prospect.
Preferably, the preparation steps of the edible fungus culture material are as follows:
weighing raw materials according to the weight ratio of 10-20 parts of yeast industrial wastewater, 60-80 parts of sawdust, 60-70 parts of corncobs, 10-15 parts of wheat bran, 20-25 parts of rice bran, 10-20 parts of cottonseed hulls, 5-20 parts of mulberry twigs, 0-1 part of cane sugar, 0.5-1.5 parts of gypsum and 0.2-0.5 part of potassium dihydrogen phosphate, uniformly mixing, and adding tap water of which the total weight is 0.6 times of that of the raw materials to obtain a first culture material.
And adding the lime water into the first mixture, and adjusting the pH value of the mixture to 5.5-7.5 to obtain a second culture material.
And subpackaging the second culture material into culture bags to a position 6-7 cm away from the bag openings, and tightly binding the bag openings to obtain a third culture material.
And naturally cooling the third culture material to room temperature after sterilization treatment to obtain the edible fungus culture material.
Preferably, the obtaining of the third compost specifically comprises: and subpackaging the second culture material into culture bags, filling the second culture material while forcibly compacting the second culture material towards the bottoms of the culture bags in the bagging process, subpackaging the second culture material to a position 6-7 cm away from the bag openings, and tightening the bag openings to obtain a third culture material.
Preferably, the sterilization treatment specifically comprises: autoclaving at 120-125 ℃ for 1-3 hours or autoclaving at 100 ℃ under normal pressure for 8-12 hours.
The invention has the advantages that:
firstly, the method for treating the yeast industrial wastewater by utilizing the edible fungus production technology mixes the yeast industrial wastewater with other raw materials to prepare the edible fungus culture material, thereby carrying out zero-cost and harmless treatment on the yeast industrial wastewater and saving the cost for specially treating the yeast industrial wastewater for the yeast industry.
Secondly, in the method for treating the yeast industrial wastewater by utilizing the edible fungus production technology, the yeast industrial wastewater is mixed with other raw materials to prepare the edible fungus culture material for cultivating the edible fungi, so that secondary economic benefits are generated, and the method has important promotion and practical value on quality improvement and efficiency improvement of the yeast industry.
In addition, in the method for treating the yeast industrial wastewater by using the edible fungus production technology, the raw materials are mixed according to a specific weight ratio to prepare the edible fungus culture material, so that the yeast industrial wastewater can be treated in time, and the proportioned edible fungus culture material can meet the growth requirement of edible fungi, thereby generating secondary economic benefit.
In addition, the method for treating the yeast industrial wastewater by using the edible fungus production technology of the invention mixes the yeast industrial wastewater with other materials to prepare the edible fungus culture material, and the obtained edible fungus culture material has wide application range, can be applied to the cultivation of various edible fungi and has good market prospect.
Detailed Description
The present invention is described in further detail below to enable those skilled in the art to practice the invention with reference to the description.
It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
It is to be noted that the experimental methods described in the following embodiments are all conventional methods unless otherwise specified, and the reagents and materials are commercially available unless otherwise specified.
Example 1
A method for treating yeast industrial wastewater by using an edible fungus production technology comprises the steps of mixing 30 kg of yeast industrial wastewater, 60 kg of sawdust, 10 kg of corncobs, 8 kg of wheat bran, 2 kg of rice bran, 5 kg of cottonseed hulls, 5 kg of mulberry twigs, 1 kg of cane sugar, 1 kg of gypsum and 0.2 kg of monopotassium phosphate to prepare an edible fungus culture material.
Example 2
A method for treating yeast industrial wastewater by using an edible fungus production technology comprises the steps of mixing 10 kg of yeast industrial wastewater, 60 kg of sawdust, 10 kg of corncobs, 8 kg of wheat bran, 2 kg of rice bran, 5 kg of cottonseed hulls, 5 kg of mulberry twigs, 1 kg of cane sugar, 1 kg of gypsum and 0.2 kg of monopotassium phosphate to prepare an edible fungus culture material.
Example 3
A method for treating yeast industrial wastewater by using an edible fungus production technology comprises the steps of mixing 20 kg of yeast industrial wastewater, 60 kg of sawdust, 5 kg of corncobs, 8 kg of wheat bran, 2 kg of rice bran, 5 kg of cottonseed hulls, 5 kg of mulberry twigs, 1 kg of cane sugar, 1 kg of gypsum and 0.2 kg of monopotassium phosphate to prepare an edible fungus culture material.
Example 4
A method for treating yeast industrial wastewater by using an edible fungus production technology is characterized in that 20 kg of yeast industrial wastewater, 60 kg of sawdust, 10 kg of corncobs, 8 kg of wheat bran, 2 kg of rice bran, 5 kg of cottonseed hulls, 1 kg of cane sugar, 1 kg of gypsum and 0.2 kg of monopotassium phosphate are mixed to prepare an edible fungus culture material.
Example 5
A method for treating yeast industrial wastewater by using an edible fungus production technology is characterized in that 20 kg of yeast industrial wastewater, 60 kg of corncobs, 10 kg of wheat bran, 10 kg of rice bran, 1 kg of gypsum and 0.2 kg of monopotassium phosphate are mixed to prepare an edible fungus culture material.
Example 6
A method for treating yeast industrial wastewater by using an edible fungus production technology comprises the step of mixing 20 kg of yeast industrial wastewater, 30 kg of sawdust, 30 kg of corncob, 10 kg of wheat bran, 10 kg of rice bran, 1 kg of cane sugar, 1 kg of gypsum and 0.2 kg of monopotassium phosphate to prepare an edible fungus culture material.
Example 7
A method for treating yeast industrial wastewater by using an edible fungus production technology comprises the step of mixing 20 kg of yeast industrial wastewater, 30 kg of sawdust, 30 kg of corncob, 20 kg of cottonseed hull, 1 kg of sucrose, 1 kg of gypsum and 0.2 kg of monopotassium phosphate to prepare an edible fungus culture material.
Example 8
A method for treating yeast industrial wastewater by using an edible fungus production technology comprises the following steps:
step one, respectively weighing 20 kg of yeast industrial wastewater, 60 kg of sawdust, 10 kg of corncobs, 8 kg of wheat bran, 2 kg of rice bran, 5 kg of cottonseed hulls, 5 kg of mulberry twigs, 1 kg of cane sugar, 1 kg of gypsum and 0.2 kg of monopotassium phosphate, uniformly mixing, and adding tap water with the weight 0.6 times of the total weight of the raw materials to obtain a first culture material.
And step two, adding the lime water into the first mixture, and adjusting the pH value of the mixture to 5.5 to obtain a second culture material.
And step three, subpackaging the second compost into culture bags to a position 7 cm away from the bag openings, and tightly binding the bag openings to obtain a third compost.
And step four, sterilizing the third culture material for 1 hour at 120 ℃ under high pressure, and naturally cooling to room temperature to obtain the edible fungus culture material.
Example 9
On the basis of the embodiment 8, the step two is replaced by the following step:
and step two, adding the lime water into the first mixture, and adjusting the pH value of the mixture to 6 to obtain a second culture material.
Example 10
On the basis of the embodiment 8, the step two is replaced by the following step:
and step two, adding the lime water into the first mixture, and adjusting the pH value of the mixture to 7.5 to obtain a second culture material.
Example 11
On the basis of the embodiment 8, the step four is replaced by the following step:
and step four, sterilizing the third culture material for 3 hours at 120 ℃ under high pressure, and naturally cooling to room temperature to obtain the edible fungus culture material.
Example 12
On the basis of the embodiment 8, the step four is replaced by the following step:
and step four, sterilizing the third culture material for 8 hours at 100 ℃ under normal pressure, and naturally cooling to room temperature to obtain the edible fungus culture material.
Comparative example 1
60 kg of sawdust, 10 kg of corncobs, 8 kg of wheat bran, 2 kg of rice bran, 5 kg of cottonseed hulls, 5 kg of mulberry twigs, 1 kg of cane sugar, 1 kg of gypsum and 0.2 kg of monopotassium phosphate are mixed to prepare the culture material for the edible fungi.
Comparative example 2
The edible fungus culture material of the embodiment 2 is obtained, the pH value of the edible fungus culture material is adjusted to 5.5 by lime water, then the edible fungus culture material is packaged into a culture bag, the culture bag is packaged to a position 7 cm away from the opening of the bag, the opening of the bag is tightened, the edible fungus culture material is naturally cooled to the room temperature after being sterilized at the temperature of 120 ℃ for 1 hour, and the edible fungus culture material is stored for 1 month at the temperature of 25 ℃.
Comparative example 3
The edible fungus culture material of example 2 was obtained, the pH value of the edible fungus culture material was adjusted to 5.5 with lime water, and then the edible fungus culture material was packed into culture bags, which were placed at a distance of 7 cm from the bag mouth, the bag mouth was tightened, and after 1 hour of autoclaving at 120 ℃, the edible fungus culture material was naturally cooled to room temperature, and stored at 25 ℃ for 3 months.
Comparative example 4
A method for treating yeast industrial wastewater comprises the steps of sequentially carrying out primary precipitation, acidification treatment, anaerobic reaction treatment, aerobic treatment, nitration treatment and secondary precipitation on the yeast industrial wastewater until discharged water bodies reach the national primary wastewater comprehensive discharge standard.
Cultivation of edible fungi Using compost
Production test
1. 100 kg of the edible fungus culture materials of the embodiments 1-7 and the comparative example 1 are respectively obtained, the pH value of the edible fungus culture materials is adjusted to be 5.5 by using lime water, the edible fungus culture materials are then packaged into culture bags with the same specification, the culture bags are packaged to be at a position 7 cm away from the bag openings, the bag openings are tightened, and the edible fungus culture materials are naturally cooled to the room temperature after being sterilized at the high pressure of 120 ℃ for 1 hour. And respectively obtaining the edible fungus culture materials of the comparative examples 2-3. Wherein the culture bag has a size of (12-18) cm x (24-35) cm.
Obtaining oyster mushroom strains, and respectively inoculating each group of culture materials, wherein the inoculation amount of each bag of culture materials is 1cm 3 And (3) tightening the opening of the culture bag again after the inoculation of the strains with the sizes is finished, and then placing the bags on a culture rack for culturing for 30 days. Wherein, the inoculation should be carried out in a disinfected inoculation box or an inoculation room, and the operation is carried out beside an alcohol lamp to prevent bacteria contamination. Before inoculation, the outer surfaces of the hands, the inoculation clamp and the strain bottle are disinfected by alcohol cotton balls; the temperature of the culture room is 15 ℃, the air humidity is 60 percent, and the conditions of light shielding and ventilation are kept.
Keeping a proper amount of scattered light 30 days after inoculation, and stimulating fruiting; after mushroom buds appear, the environmental temperature is kept at 20 ℃ and the humidity is kept at 70%, and a proper amount of scattered light is kept until the entity is mature, so that harvesting can be carried out.
After harvesting, cleaning residual stipes, broken mushrooms, dead mushrooms and the like with a sterilized tool, keeping the fruiting mouth dry for two or three days, and then spraying water to promote the second tide of mushrooms to germinate. Wherein the temperature and humidity condition is 15-25 deg.C, and the humidity is 60-90%.
And respectively recording the total harvesting weight of each group of oyster mushrooms, and calculating the cultivation cost of the oyster mushrooms and the net income after yeast industrial wastewater treatment. The results are shown in table 1: (wherein, the total cost is raw material cost plus labor cost, the wholesale price of sawdust is 200 yuan/ton, the wholesale price of corncobs is 600 yuan/ton, the wholesale price of wheat bran is 1500 yuan/ton, the wholesale price of rice bran is 3000 yuan/ton, the wholesale price of cottonseed hulls is 2000 yuan/ton, gypsum is 1000 yuan/ton, the wholesale price of white granulated sugar is 5400 yuan/ton, the cost is not calculated by adopting trimmed waste branches for mulberry branches, the labor cost is 1500-2500 yuan/ton, the net income is oyster mushroom yield multiplied by unit price-total cost, the wholesale price of oyster mushroom is 5000 yuan-6000 yuan/ton when harvesting.)
As can be seen from the data in Table 1, the method of the invention is used for treating the yeast industrial wastewater and preparing the edible fungi culture material to cultivate the oyster mushroom, the net benefit is objective, and the secondary economic benefit can be obviously generated.
2. 1 ton of yeast industrial wastewater was treated by the method of comparative example 4, and the treatment cost was 10 Yuan/ton.
By adopting the data of the example 2 in the table 1 for conversion, the edible fungi produced by 1 ton of yeast industrial wastewater can generate about 42600 yuan net income, and has important promotion and practical value for improving the quality and the efficiency of the yeast industry.
Second, detecting the nutrient content
The oyster mushrooms obtained by cultivating the edible fungus culture materials prepared in the examples 1-3 and the comparative example 1 are divided into 4 groups, and the nutrients of the 4 groups of oyster mushrooms are respectively detected according to a Coomassie brilliant blue method, the content measurement of crude polysaccharide in NY/T1676-:
| item | Soluble protein content% | Content of polysaccharide% | Total amino acid content% |
| Example 1 | 10.93 | 8.30 | 16.3 |
| Example 2 | 13.73 | 8.66 | 18.4 |
| Example 3 | 10.82 | 7.92 | 16.2 |
| Example 4 | 10.80 | 7.96 | 14.6 |
| Example 5 | 13.50 | 7.21 | 18.3 |
| Example 6 | 13.27 | 7.32 | 17.9 |
| Example 7 | 9.24 | 7.65 | 13.6 |
| Example 8 | 8.06 | 8.29 | 13.2 |
| Comparative example 1 | 10.72 | 3.29 | 15.8 |
As can be seen from the data in Table 2, the polysaccharide content and the total amino acid content of the oyster mushrooms cultivated by the methods of examples 1 to 8 are higher than those of the oyster mushrooms cultivated by the comparative examples. The soluble protein content, polysaccharide content and total amino acid content of the oyster mushroom obtained by cultivating the edible fungus culture material prepared according to the raw material proportion in the embodiment 2 are highest.
While embodiments of the invention have been disclosed above, it is not intended to be limited to the uses set forth in the specification and examples. It can be applied to all kinds of fields suitable for the present invention. Additional modifications will readily occur to those skilled in the art. The invention is therefore not to be limited to the specific details described herein, without departing from the general concept as defined by the appended claims and their equivalents.
Claims (9)
1. A method for processing yeast industrial wastewater by utilizing edible fungus production technology is characterized in that the following raw materials are mixed to prepare edible fungus culture material; the raw materials comprise:
yeast industrial wastewater, gypsum, monopotassium phosphate and sucrose;
and at least one of wood chips, corncobs and mulberry twigs;
and at least one of wheat bran, rice bran and cottonseed hull;
wherein the mass percentage concentration of the yeast industrial wastewater in the edible fungus culture material is 5-40%.
2. The method for treating the yeast industrial wastewater by using the edible fungus production technology as claimed in claim 1, wherein the mass percentage concentration of the yeast industrial wastewater in the edible fungus culture material is 10-20%.
3. The method for treating yeast industrial wastewater by using the edible fungus production technology as claimed in claim 1, wherein the edible fungus culture material comprises the following raw materials in parts by weight:
10-20 parts of yeast industrial wastewater, 0-80 parts of sawdust, 0-80 parts of corncob, 0-30 parts of wheat bran, 0-30 parts of rice bran, 0-20 parts of cottonseed hull, 0-60 parts of mulberry twig, 0-1 part of cane sugar, 0.5-1.5 parts of gypsum and 0.2-0.5 part of potassium dihydrogen phosphate.
4. The method for treating yeast industrial wastewater by using the edible fungus production technology as claimed in claim 1, wherein the edible fungus culture material specifically comprises the following raw materials in parts by weight:
10 parts of yeast industrial wastewater, 60 parts of sawdust, 10 parts of corncobs, 8 parts of wheat bran, 2 parts of rice bran, 5 parts of cottonseed hulls, 5 parts of mulberry twigs, 1 part of cane sugar, 1 part of gypsum and 0.2 part of monopotassium phosphate.
5. The method for treating yeast industrial wastewater by using the edible fungus production technology as claimed in claim 1, wherein the pH value of the edible fungus culture material is 5.5-7.5.
6. The method for treating yeast industrial wastewater by using an edible fungi production technology as claimed in claim 1, wherein the edible fungi culture material can be used for cultivation of oyster mushroom, shiitake mushroom, pleurotus eryngii, pleurotus geesteranus or pleurotus citrinopileatus.
7. The method for treating yeast industrial wastewater by using the edible fungus production technology as claimed in claim 1, wherein the preparation step of the edible fungus culture material is as follows:
weighing raw materials according to the weight ratio of 10-20 parts of yeast industrial wastewater, 0-80 parts of sawdust, 0-80 parts of corncobs, 0-30 parts of wheat bran, 0-30 parts of rice bran, 0-20 parts of cottonseed hulls, 0-60 parts of mulberry twigs, 0-1 part of cane sugar, 0.5-1.5 parts of gypsum and 0.2-0.5 part of potassium dihydrogen phosphate, uniformly mixing, and adding tap water of which the total weight is 0.6 times of that of the raw materials to obtain a first culture material;
adding lime water into the first mixture, and adjusting the pH value of the mixture to 5.5-7.5 to obtain a second culture material;
subpackaging the second compost into culture bags, subpackaging the bags to a position 6-7 cm away from the bag openings, and tightly binding the bag openings to obtain a third compost;
and naturally cooling the third culture material to room temperature after sterilization treatment to obtain the edible fungus culture material.
8. The method for treating yeast industrial wastewater by using an edible fungus production technology as claimed in claim 7, wherein the step of obtaining the third culture medium specifically comprises the steps of:
and subpackaging the second culture material into culture bags, filling the second culture material while forcibly compacting the second culture material towards the bottoms of the culture bags in the bagging process, subpackaging the second culture material to a position 6-7 cm away from the bag openings, and tightening the bag openings to obtain a third culture material.
9. The method for treating yeast industrial wastewater by using an edible fungus production technology as claimed in claim 7, wherein the sterilization treatment specifically comprises: autoclaving at 120-125 ℃ for 1-3 hours or autoclaving at 100 ℃ under normal pressure for 8-12 hours.
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