CN108610112A - A kind of new formula and its method of cultivation elegant precious mushroom - Google Patents
A kind of new formula and its method of cultivation elegant precious mushroom Download PDFInfo
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- CN108610112A CN108610112A CN201611107538.8A CN201611107538A CN108610112A CN 108610112 A CN108610112 A CN 108610112A CN 201611107538 A CN201611107538 A CN 201611107538A CN 108610112 A CN108610112 A CN 108610112A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
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Abstract
The invention belongs to fungus growing technique fields, including a kind of elegant precious mushroom cultivation matrix and preparation method thereof.A kind of new matrix is made of husk, cotton seed hulls, wheat bran, corn flour, lime, gypsum.Basal culture medium matter has the features such as preparing simply, material source is wide, mycelia growth is fast.Contain abundant nutriment in dispensing of the present invention, guarantee is provided for the good growth of elegant precious mushroom.Solves the problems, such as south cultivation elegant precious mushroom shortage of raw materials.
Description
Technical field
The present invention relates to a kind of elegant precious mushroom cultivation matrixes and preparation method thereof, and cultivate elegant precious mushroom using the culture medium
Method.
Background technology
Elegant precious mushroom shape is pleasing, mouthfeel is tender and clear and melodious, delicious flavour, rich in protein in fresh mushroom, and contains human body
8 kinds of required essential amino acids.Cultivation matrix is that we produce the basis of elegant precious mushroom, is factory produced and needs to consume a large amount of plant
Train matrix.With flourishing for mushroom industry in recent years, the elegant precious mushrooms cultivation matrix such as traditional cotton seed hulls, sawdust is in China
The problems such as southern area price goes up year by year, and poor quality, and yield is few.Therefore, research and development amount is easy to get greatly, cheap replacement
Matrix is the key that elegant precious mushroom cultivation regional further genralrlization cultivation in south China
Elegant precious mushroom is domestomycetes, and the carbon source utilized is mainly lignin and cellulose.Rice as China's staple food crop it
One, annual yield is more than 2 × 108Ton, husk account for the 20% of paddy weight, can generate 4 × 10 every year7Ton.Currently, husk conduct
Using energy source is the best way, and main is exactly as a kind of fuel.However a large amount of husk burning can cause environment very greatly
Pollution, increase the content of PM2.5 in air, phenomena such as causing haze.If can be using husk as the culture material of edible mushroom, not only
The utilization ways of the efficient green of a husk can be increased, moreover it is possible to provide a large amount of former materials cheap and easy to get for edible fungus culturing
Material.This measure can promote the development of mushroom industry, bring economic benefit to us, while the problem that can reduce environmental pollution
Up to the present, the document report that elegant precious mushroom is cultivated using husk as raw material is also found no.Its critical issue is,
There is no the cultural method of suitable culture material formula and ripe high yield.
Invention content
It is an object of the invention to find, a kind of yield is high, price is low, the simple elegant precious mushroom culturing raw material of cultural method.It carries
As new cultivation matrix of elegant precious mushroom and preparation method thereof and the cultivation matrix is utilized to cultivate using husk and cotton seed hulls for a kind of
The method of elegant precious mushroom.It is easy to learn using the method for the substrate culture oyster mushroom, not high to equipment and site requirements, and pollution rate
Low, yield is high, meanwhile, it proposes an approach for rationally utilizing husk, turns waste into wealth, you can increasing the income of mushroom grower can protect again
Ecological environment
In order to achieve the above object, the present invention is realized by following scheme:One kind cultivating elegant precious mushroom using husk and cotton seed hulls as major ingredient
New matrix, the advantage is that its ingredient and mass percent are:Husk accounts for 0% ~ 75%, and cotton seed hulls accounts for 0% ~ 75%, and wheat bran accounts for
16.5%, corn flour accounts for 5%, lime 2%, gypsum 1.5%
A method of elegant precious mushroom being cultivated as major ingredient using husk and cotton seed hulls, which is characterized in that include the following steps:
Step 1 is accounted for 0% ~ 75% by husk, and cotton seed hulls accounts for 0% ~ 75%, and wheat bran accounts for 16.5%, and corn flour accounts for 5%, lime 2%, gypsum
1.5% ratio mixing.(Above each component ratio is mass percent)
Step 2, spice pack, above-mentioned formula culture medium is fully mixed thoroughly, and moisture content in medium control is 60% ~ 65% into luggage
Bag, per packed 400 ~ 500g of culture medium;
Step 3, sterilizing, using 121 DEG C of high pressure steam sterilization 100min of temperature;
Step 4, cooling, inoculation, when cooling down to room temperature, are inoculated with, about 20 ~ 30 mycelium pellets of inoculum concentration;
Step 5, cultural hypha, culture mycelia 20 days to 30 days under 15 ~ 25 DEG C, the dark surrounds of 30 ~ 50% air humiditys;
Step 6, flower bud, opening bacterium bag stimulates it using the temperature difference under the conditions of air humidity 70 ~ 80%;
Step 7, management of producing mushroom are grown 2 ~ 3 days under the conditions of 20 ~ 27 DEG C, air humidity 85 ~ 90%;
First batch of step 8, harvesting mushroom;
Step 9, bacteria, moisturizing, culture mycelia 3 ~ 5 days under 15 ~ 25 DEG C, the dark surrounds of 30 ~ 50% air humiditys, later,
Under air humidity 70 ~ 80%, flower bud is carried out using thermal stimulation;
Step 10, management of producing mushroom are grown 2 ~ 3 days under the conditions of 20 ~ 27 DEG C, air humidity 85 ~ 90%;
Second batch of step 11, harvesting mushroom
Step 12, bacteria, moisturizing, culture mycelia 3 ~ 5 days, Zhi Houti under 15 ~ 25 DEG C, the dark surrounds of 30 ~ 50% air humiditys
High air humidity carries out flower bud to 70 ~ 80%, using the temperature difference;
Step 13, management of producing mushroom are grown 2 ~ 3 days under the conditions of 20 ~ 27 DEG C, air humidity 85 ~ 90%;
Step 14, harvesting third stubble mushroom
Step 15, bacteria, moisturizing, culture mycelia 3 ~ 5 days, Zhi Houti under 15 ~ 25 DEG C, the dark surrounds of 30 ~ 50% air humiditys
High air humidity carries out flower bud to 70 ~ 80%, using the temperature difference;
Step 16, management of producing mushroom are grown 2 ~ 3 days under the conditions of 20 ~ 27 DEG C, air humidity 85 ~ 90%;
The 4th batch of step 17, harvesting mushroom
Compared with prior art, the present invention haing the following advantages:
(1)Husk is the by-product of paddy processing, and yield is high, source is wide, cheap, is that the waste in Rice Production follows again
Ring
(2)Less investment, it is at low cost.Matrix preparation manipulation is simple and practicable, and dispersion prepares or scale
(3)Good product quality, remarkable in economical benefits, the market competitiveness are strong
(4)It is high can to solve elegant precious mushroom culture material shortage, price, is mushroom grower additional income.Protection is played to ecological environment simultaneously
Effect
Description of the drawings:
Attached drawing 1:Elegant precious mushroom of the present invention cultivates new matrix preparation flow figure
Attached drawing 2:Utilize the flow chart of the new substrate culture elegant precious mushroom of the present invention
Specific implementation mode:The present invention be directed to southern region of China can not be taken on the spot with traditional cottonseed shell cultivation elegant precious mushroom
The problems such as material and price goes up year after year, cost increases and this abundant resource of husk fail to be utilized effectively, for a long time
Since can only be used as fuel, cause the waste of resource and ecological environment polluted.It is that major ingredient is precious as show using husk
The cultivation matrix of mushroom solves southern area husk Utilizing question and southern area elegant precious mushroom culture material and lacks and of high cost
Problem also provides a new approach for agricultural waste material cycling and reutilization.Experiment, which is repeated several times, to be proved, husk completely may be used
To cultivate elegant precious mushroom as major ingredient.The effect of husk and cotton seed hulls different proportion mixed culture elegant precious mushroom is shown in Table 1
The elegant precious mushroom cultivation effect of 1 husk of table and the mixing of cotton seed hulls different quality ratio
Cotton seed hulls % | Husk % | Wheat bran % | Corn flour % | Gypsum % | Lime % | Biological transformation ratio |
75 | 0 | 16.5 | 5 | 1.5 | 2 | 74.98 |
67.5 | 7.5 | 16.5 | 5 | 1.5 | 2 | 47.76 |
60 | 15 | 16.5 | 5 | 1.5 | 2 | 54.59 |
52.5 | 22.5 | 16.5 | 5 | 1.5 | 2 | 51.64 |
45 | 30 | 16.5 | 5 | 1.5 | 2 | 50.96 |
37.5 | 37.5 | 16.5 | 5 | 1.5 | 2 | 55.59 |
30 | 45 | 16.5 | 5 | 1.5 | 2 | 43.29 |
22.5 | 52.5 | 16.5 | 5 | 1.5 | 2 | 55.87 |
15 | 60 | 16.5 | 5 | 1.5 | 2 | 37.86 |
7.5 | 67.5 | 16.5 | 5 | 1.5 | 2 | 33.82 |
0 | 75 | 16.5 | 5 | 1.5 | 2 | 30.25 |
Case study on implementation 1:A kind of new method with husk and cottonseed shell cultivation elegant precious mushroom includes the following steps:
Step 1 is accounted for 15% by husk, and cotton seed hulls accounts for 60%, and wheat bran accounts for 16.5%, and corn flour accounts for 5%, lime 2%, the ratio of gypsum 1.5%
Example mixing.(Above each component ratio is mass percent)
Step 2, spice pack, above-mentioned formula culture medium is fully mixed thoroughly, and moisture content in medium control is 60% ~ 65% into luggage
Bag, per packed 400 ~ 500g of culture medium;
Step 3, sterilizing, using 121 DEG C of high pressure steam sterilization 100min of temperature;
Step 4, cooling, inoculation, when cooling down to room temperature, are inoculated with, about 20 ~ 30 mycelium pellets of inoculum concentration;
Step 5, cultural hypha, culture mycelia 20 days to 30 days under 15 ~ 25 DEG C, the dark surrounds of 30 ~ 50% air humiditys;
Step 6, flower bud, opening bacterium bag stimulates it using the temperature difference under the conditions of air humidity 70 ~ 80%;
Step 7, management of producing mushroom are grown 2 ~ 3 days under the conditions of 20 ~ 27 DEG C, air humidity 85 ~ 90%;
First batch of step 8, harvesting mushroom;
Step 9, bacteria, moisturizing, culture mycelia 3 ~ 5 days under 15 ~ 25 DEG C, the dark surrounds of 30 ~ 50% air humiditys, later,
Under air humidity 70 ~ 80%, flower bud is carried out using thermal stimulation;
Step 10, management of producing mushroom are grown 2 ~ 3 days under the conditions of 20 ~ 27 DEG C, air humidity 85 ~ 90%;
Second batch of step 11, harvesting mushroom
Step 12, bacteria, moisturizing, culture mycelia 3 ~ 5 days, Zhi Houti under 15 ~ 25 DEG C, the dark surrounds of 30 ~ 50% air humiditys
High air humidity carries out flower bud to 70 ~ 80%, using the temperature difference;
Step 13, management of producing mushroom are grown 2 ~ 3 days under the conditions of 20 ~ 27 DEG C, air humidity 85 ~ 90%;
Step 14, harvesting third stubble mushroom
Step 15, bacteria, moisturizing, culture mycelia 3 ~ 5 days, Zhi Houti under 15 ~ 25 DEG C, the dark surrounds of 30 ~ 50% air humiditys
High air humidity carries out flower bud to 70 ~ 80%, using the temperature difference;
Step 16, management of producing mushroom are grown 2 ~ 3 days under the conditions of 20 ~ 27 DEG C, air humidity 85 ~ 90%;
The 4th batch of step 17, harvesting mushroom
Table 2 cultivates elegant precious mushroom effect using 15% husk and 60% cotton seed hulls as major ingredient
Case study on implementation 2:A kind of new method with husk and cottonseed shell cultivation elegant precious mushroom includes the following steps:
Step 1 is accounted for 22.5% by husk, and cotton seed hulls accounts for 52.5%, and wheat bran accounts for 16.5%, and corn flour accounts for 5%, lime 2%, gypsum 1.5%
Ratio mixing.(Above each component ratio is mass percent)
Step 2, spice pack, above-mentioned formula culture medium is fully mixed thoroughly, and moisture content in medium control is 60% ~ 65% into luggage
Bag, per packed 400 ~ 500g of culture medium;
Step 3, sterilizing, using 121 DEG C of high pressure steam sterilization 100min of temperature;
Step 4, cooling, inoculation, when cooling down to room temperature, are inoculated with, about 20 ~ 30 mycelium pellets of inoculum concentration;
Step 5, cultural hypha, culture mycelia 20 days to 30 days under 15 ~ 25 DEG C, the dark surrounds of 30 ~ 50% air humiditys;
Step 6, flower bud, opening bacterium bag stimulates it using the temperature difference under the conditions of air humidity 70 ~ 80%;
Step 7, management of producing mushroom are grown 2 ~ 3 days under the conditions of 20 ~ 27 DEG C, air humidity 85 ~ 90%;
First batch of step 8, harvesting mushroom;
Step 9, bacteria, moisturizing, culture mycelia 3 ~ 5 days under 15 ~ 25 DEG C, the dark surrounds of 30 ~ 50% air humiditys, later,
Under air humidity 70 ~ 80%, flower bud is carried out using thermal stimulation;
Step 10, management of producing mushroom are grown 2 ~ 3 days under the conditions of 20 ~ 27 DEG C, air humidity 85 ~ 90%;
Second batch of step 11, harvesting mushroom
Step 12, bacteria, moisturizing, culture mycelia 3 ~ 5 days, Zhi Houti under 15 ~ 25 DEG C, the dark surrounds of 30 ~ 50% air humiditys
High air humidity carries out flower bud to 70 ~ 80%, using the temperature difference;
Step 13, management of producing mushroom are grown 2 ~ 3 days under the conditions of 20 ~ 27 DEG C, air humidity 85 ~ 90%;
Step 14, harvesting third stubble mushroom
Step 15, bacteria, moisturizing, culture mycelia 3 ~ 5 days, Zhi Houti under 15 ~ 25 DEG C, the dark surrounds of 30 ~ 50% air humiditys
High air humidity carries out flower bud to 70 ~ 80%, using the temperature difference;
Step 16, management of producing mushroom are grown 2 ~ 3 days under the conditions of 20 ~ 27 DEG C, air humidity 85 ~ 90%;
The 4th batch of step 17, harvesting mushroom
Table 3 cultivates elegant precious mushroom effect using 22.5% husk and 52.5% cotton seed hulls as major ingredient
Case study on implementation 3:A kind of new method with husk and cottonseed shell cultivation elegant precious mushroom includes the following steps:
Step 1 is accounted for 37.5% by husk, and cotton seed hulls accounts for 37.5%, and wheat bran accounts for 16.5%, and corn flour accounts for 5%, lime 2%, gypsum 1.5%
Ratio mixing.(Above each component ratio is mass percent)
Step 2, spice pack, above-mentioned formula culture medium is fully mixed thoroughly, and moisture content in medium control is 60% ~ 65% into luggage
Bag, per packed 400 ~ 500g of culture medium;
Step 3, sterilizing, using 121 DEG C of high pressure steam sterilization 100min of temperature;
Step 4, cooling, inoculation, when cooling down to room temperature, are inoculated with, about 20 ~ 30 mycelium pellets of inoculum concentration;
Step 5, cultural hypha, culture mycelia 20 days to 30 days under 15 ~ 25 DEG C, the dark surrounds of 30 ~ 50% air humiditys;
Step 6, flower bud, opening bacterium bag stimulates it using the temperature difference under the conditions of air humidity 70 ~ 80%;
Step 7, management of producing mushroom are grown 2 ~ 3 days under the conditions of 20 ~ 27 DEG C, air humidity 85 ~ 90%;
First batch of step 8, harvesting mushroom
Step 9, bacteria, moisturizing, culture mycelia 3 ~ 5 days under 15 ~ 25 DEG C, the dark surrounds of 30 ~ 50% air humiditys, later,
Under air humidity 70 ~ 80%, flower bud is carried out using thermal stimulation;
Step 10, management of producing mushroom are grown 2 ~ 3 days under the conditions of 20 ~ 27 DEG C, air humidity 85 ~ 90%;
Second batch of step 11, harvesting mushroom
Step 12, bacteria, moisturizing, culture mycelia 3 ~ 5 days, Zhi Houti under 15 ~ 25 DEG C, the dark surrounds of 30 ~ 50% air humiditys
High air humidity carries out flower bud to 70 ~ 80%, using the temperature difference;
Step 13, management of producing mushroom are grown 2 ~ 3 days under the conditions of 20 ~ 27 DEG C, air humidity 85 ~ 90%;
Step 14, harvesting third stubble mushroom
Step 15, bacteria, moisturizing, culture mycelia 3 ~ 5 days, Zhi Houti under 15 ~ 25 DEG C, the dark surrounds of 30 ~ 50% air humiditys
High air humidity carries out flower bud to 70 ~ 80%, using the temperature difference;
Step 16, management of producing mushroom are grown 2 ~ 3 days under the conditions of 20 ~ 27 DEG C, air humidity 85 ~ 90%;
The 4th batch of step 17, harvesting mushroom
Table 4 cultivates elegant precious mushroom effect using 37.5% husk and 37.5 % cotton seed hulls as major ingredient
Case study on implementation 3:A kind of new method with husk and cottonseed shell cultivation elegant precious mushroom includes the following steps:
Step 1 is accounted for 52.5% by husk, and cotton seed hulls accounts for 22.5%, and wheat bran accounts for 16.5%, and corn flour accounts for 5%, lime 2%, gypsum 1.5%
Ratio mixing.(Above each component ratio is mass percent)
Step 2, spice pack, above-mentioned formula culture medium is fully mixed thoroughly, and moisture content in medium control is 60% ~ 65% into luggage
Bag, per packed 400 ~ 500g of culture medium;
Step 3, sterilizing, using 121 DEG C of high pressure steam sterilization 100min of temperature;
Step 4, cooling, inoculation, when cooling down to room temperature, are inoculated with, about 20 ~ 30 mycelium pellets of inoculum concentration;
Step 5, cultural hypha, culture mycelia 20 days to 30 days under 15 ~ 25 DEG C, the dark surrounds of 30 ~ 50% air humiditys;
Step 6, flower bud, opening bacterium bag stimulates it using the temperature difference under the conditions of air humidity 70 ~ 80%;
Step 7, management of producing mushroom are grown 2 ~ 3 days under the conditions of 20 ~ 27 DEG C, air humidity 85 ~ 90%;
First batch of step 8, harvesting mushroom;
Step 9, bacteria, moisturizing, culture mycelia 3 ~ 5 days under 15 ~ 25 DEG C, the dark surrounds of 30 ~ 50% air humiditys, later,
Under air humidity 70 ~ 80%, flower bud is carried out using thermal stimulation;
Step 10, management of producing mushroom are grown 2 ~ 3 days under the conditions of 20 ~ 27 DEG C, air humidity 85 ~ 90%;
Second batch of step 11, harvesting mushroom
Step 12, bacteria, moisturizing, culture mycelia 3 ~ 5 days, Zhi Houti under 15 ~ 25 DEG C, the dark surrounds of 30 ~ 50% air humiditys
High air humidity carries out flower bud to 70 ~ 80%, using the temperature difference;
Step 13, management of producing mushroom are grown 2 ~ 3 days under the conditions of 20 ~ 27 DEG C, air humidity 85 ~ 90%;
Step 14, harvesting third stubble mushroom
Step 15, bacteria, moisturizing, culture mycelia 3 ~ 5 days, Zhi Houti under 15 ~ 25 DEG C, the dark surrounds of 30 ~ 50% air humiditys
High air humidity carries out flower bud to 70 ~ 80%, using the temperature difference;
Step 16, management of producing mushroom are grown 2 ~ 3 days under the conditions of 20 ~ 27 DEG C, air humidity 85 ~ 90%;
The 4th batch of step 17, harvesting mushroom
Table 5 cultivates elegant precious mushroom effect using 52.5% husk and 22.5 % cotton seed hulls as major ingredient
Claims (2)
1. a kind of method for cultivating elegant precious mushroom as major ingredient using husk and cotton seed hulls, which is characterized in that its ingredient and quality percentage
Than accounting for 0% ~ 75% for husk, cotton seed hulls accounts for 0% ~ 75%, and wheat bran accounts for 16.5%, and corn flour accounts for 5%, lime 2%, gypsum 1.5%.
2. using the elegant precious mushroom cultural method for the new matrix that elegant precious mushroom described in claim 1 is cultivated, include the following steps;
Step 1 is accounted for 0% ~ 75% by husk, and cotton seed hulls accounts for 0% ~ 75%, and wheat bran accounts for 16.5%, and corn flour accounts for 5%, lime 2%, gypsum
1.5% ratio mixing(Above each component ratio is mass percent)
Step 2, spice pack, above-mentioned formula culture medium is fully mixed thoroughly, and moisture content in medium control is 60% ~ 65% into luggage
Bag, per packed 400 ~ 500g of culture medium;
Step 3, sterilizing, using 121 DEG C of high pressure steam sterilization 100min of temperature;
Step 4, cooling, inoculation, when cooling down to room temperature, are inoculated with, about 20 ~ 30 mycelium pellets of inoculum concentration;
Step 5, cultural hypha, culture mycelia 20 days to 30 days under 15 ~ 25 DEG C, the dark surrounds of 30 ~ 50% air humiditys;
Step 6, flower bud, opening bacterium bag stimulates it using the temperature difference under the conditions of air humidity 70 ~ 80%;
Step 7, management of producing mushroom are grown 2 ~ 3 days under the conditions of 20 ~ 27 DEG C, air humidity 85 ~ 90%;
First batch of step 8, harvesting mushroom;
Step 9, bacteria, moisturizing, culture mycelia 3 ~ 5 days under 15 ~ 25 DEG C, the dark surrounds of 30 ~ 50% air humiditys, later,
Under air humidity 70 ~ 80%, flower bud is carried out using thermal stimulation;
Step 10, management of producing mushroom are grown 2 ~ 3 days under the conditions of 20 ~ 27 DEG C, air humidity 85 ~ 90%;
Second batch of step 11, harvesting mushroom
Step 12, bacteria, moisturizing, culture mycelia 3 ~ 5 days, Zhi Houti under 15 ~ 25 DEG C, the dark surrounds of 30 ~ 50% air humiditys
High air humidity carries out flower bud to 70 ~ 80%, using the temperature difference;
Step 13, management of producing mushroom are grown 2 ~ 3 days under the conditions of 20 ~ 27 DEG C, air humidity 85 ~ 90%;
Step 14, harvesting third stubble mushroom
Step 15, bacteria, moisturizing, culture mycelia 3 ~ 5 days, Zhi Houti under 15 ~ 25 DEG C, the dark surrounds of 30 ~ 50% air humiditys
High air humidity carries out flower bud to 70 ~ 80%, using the temperature difference;
Step 16, management of producing mushroom are grown 2 ~ 3 days under the conditions of 20 ~ 27 DEG C, air humidity 85 ~ 90%;
The 4th batch of step 17, harvesting mushroom.
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106957189A (en) * | 2017-03-29 | 2017-07-18 | 东莞爱尚菇食品科技有限公司 | The Frost's Descent flat fine and soft mushroom large-scale planting method |
CN109168961A (en) * | 2018-10-31 | 2019-01-11 | 芒市喜丰农业发展有限公司 | A kind of cultural method of white ginseng |
CN114830967A (en) * | 2022-05-17 | 2022-08-02 | 湖南苇业农业生态科技有限公司 | Pleurotus geesteranus culture medium and preparation method thereof |
-
2016
- 2016-12-06 CN CN201611107538.8A patent/CN108610112A/en active Pending
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106957189A (en) * | 2017-03-29 | 2017-07-18 | 东莞爱尚菇食品科技有限公司 | The Frost's Descent flat fine and soft mushroom large-scale planting method |
CN109168961A (en) * | 2018-10-31 | 2019-01-11 | 芒市喜丰农业发展有限公司 | A kind of cultural method of white ginseng |
CN114830967A (en) * | 2022-05-17 | 2022-08-02 | 湖南苇业农业生态科技有限公司 | Pleurotus geesteranus culture medium and preparation method thereof |
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Application publication date: 20181002 |