CN114560725A - Livestock and poultry manure rapid heating and decomposing and DOM extracting method based on microbial regulation - Google Patents
Livestock and poultry manure rapid heating and decomposing and DOM extracting method based on microbial regulation Download PDFInfo
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- CN114560725A CN114560725A CN202210407580.0A CN202210407580A CN114560725A CN 114560725 A CN114560725 A CN 114560725A CN 202210407580 A CN202210407580 A CN 202210407580A CN 114560725 A CN114560725 A CN 114560725A
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- 239000010871 livestock manure Substances 0.000 title claims abstract description 88
- 210000003608 fece Anatomy 0.000 title claims abstract description 76
- 244000144972 livestock Species 0.000 title claims abstract description 46
- 244000144977 poultry Species 0.000 title claims abstract description 46
- 230000000813 microbial effect Effects 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 26
- 238000010438 heat treatment Methods 0.000 title claims abstract description 10
- 238000000855 fermentation Methods 0.000 claims abstract description 36
- 230000004151 fermentation Effects 0.000 claims abstract description 36
- 239000000047 product Substances 0.000 claims abstract description 18
- 238000009264 composting Methods 0.000 claims abstract description 17
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims abstract description 16
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 16
- 239000000284 extract Substances 0.000 claims abstract description 13
- 238000001914 filtration Methods 0.000 claims abstract description 10
- 238000002156 mixing Methods 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 230000035784 germination Effects 0.000 claims abstract description 8
- 239000006228 supernatant Substances 0.000 claims abstract description 7
- 238000003756 stirring Methods 0.000 claims abstract description 5
- 241000287828 Gallus gallus Species 0.000 claims description 9
- 241001494479 Pecora Species 0.000 claims description 9
- 240000006439 Aspergillus oryzae Species 0.000 claims description 4
- 235000002247 Aspergillus oryzae Nutrition 0.000 claims description 4
- 244000063299 Bacillus subtilis Species 0.000 claims description 4
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 4
- 241001313536 Thermothelomyces thermophila Species 0.000 claims description 4
- 238000000605 extraction Methods 0.000 claims description 4
- 239000012528 membrane Substances 0.000 claims description 4
- 238000005119 centrifugation Methods 0.000 claims description 3
- 238000001471 micro-filtration Methods 0.000 claims description 2
- 239000000203 mixture Substances 0.000 abstract description 7
- 238000000354 decomposition reaction Methods 0.000 abstract description 6
- 239000002068 microbial inoculum Substances 0.000 abstract description 4
- 239000002028 Biomass Substances 0.000 abstract description 3
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 2
- 238000004064 recycling Methods 0.000 abstract description 2
- 241000283690 Bos taurus Species 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 238000005034 decoration Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000003895 organic fertilizer Substances 0.000 description 2
- 230000007226 seed germination Effects 0.000 description 2
- 238000010564 aerobic fermentation Methods 0.000 description 1
- 239000002154 agricultural waste Substances 0.000 description 1
- 239000010828 animal waste Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/70—Controlling the treatment in response to process parameters
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Tropical Medicine & Parasitology (AREA)
- Processing Of Solid Wastes (AREA)
- Fertilizers (AREA)
Abstract
The invention relates to a method for quickly heating and decomposing livestock and poultry manure and extracting DOM (document object model) based on microbial regulation, belonging to the technical field of biomass recycling. The method comprises the following steps: 1) mixing livestock and poultry manure, mushroom residues and a microbial agent to obtain a premix; 2) carrying out composting fermentation on the premix, controlling the highest fermentation temperature of the composting fermentation to be 60-70 ℃, and turning and stirring every 3 days; 3) when the germination index of the stacked seeds is more than 70 percent, a livestock and poultry manure decomposed product is obtained; 4) and mixing the livestock and poultry manure decomposed product with water, shaking, centrifuging, taking supernatant, and filtering to obtain a DOM extract. The method is simple to operate, the livestock and poultry manure is subjected to resource treatment, and large-scale production can be realized; and moreover, the heap body added with the microbial inoculum has the advantages of quick temperature rise, capability of maintaining continuous high-temperature and high-efficiency fermentation, short decomposition period, rich extracted DOM composition and high yield.
Description
Technical Field
The invention relates to the technical field of biomass recycling, in particular to a method for quickly heating and decomposing livestock and poultry manure and extracting DOM (document object model) based on microbial regulation.
Background
The livestock manure is a main component in agricultural wastes in China, is a potential recyclable resource, and can be directly used as a raw material to participate in composting and fermentation to produce an organic fertilizer. Among the various disposal modes of the livestock and poultry manure, the aerobic fermentation is most widely applied. There are still some key technical challenges to overcome. For example, the rapid temperature rise and the continuous high temperature maintenance of the stack in the natural fermentation are important factors for restricting the high-efficiency fermentation of the livestock and poultry manure. On the other hand, from 2021, China implemented the new standard NY/T525-2021 of the organic fertilizer industry. The new standard pays more attention to the safety of the product and the maturity of the livestock and poultry manure. Therefore, the improvement of the livestock and poultry manure decomposition technology and the cleaning improvement of the fermentation process are imperative.
Disclosure of Invention
The invention aims to provide a method for rapidly heating and decomposing livestock and poultry manure and extracting DOM (document object model) based on microbial regulation. The invention utilizes the microbial inoculum to regulate and optimize the livestock manure decomposition process, thereby obtaining the DOM extract with rich content. The microbial control of the decomposition of the livestock and poultry manure can expand the high-value conversion of the livestock and poultry manure and realize the effective utilization of biomass resources. The method of the invention mixes and ferments the animal waste and the mushroom dregs, on one hand, the problem of reasonable disposal of the two wastes can be solved, and on the other hand, high-value extracts can be produced.
The invention provides a method for quickly heating and decomposing livestock and poultry manure and extracting DOM (document object model) based on microbial regulation, which comprises the following steps of:
1) mixing livestock and poultry manure, mushroom residues and a microbial agent to obtain a premix; the addition amount of the microbial agent is 10% of the total mass of the livestock and poultry manure and the mushroom residue;
2) carrying out composting fermentation on the premix, controlling the highest fermentation temperature of the composting fermentation to be 60-70 ℃, and turning and stirring every 3 days;
3) when the germination index of the stacked seeds is more than 70 percent, a livestock and poultry manure decomposed product is obtained;
4) and mixing the livestock and poultry manure decomposed product with water, shaking, centrifuging, taking supernatant, and filtering to obtain a DOM extract.
Preferably, the mass ratio of the livestock and poultry manure to the mushroom dregs is 4: 1.
Preferably, the livestock manure comprises one or more of chicken manure, sheep manure and cow manure.
Preferably, the livestock and poultry manure comprises the following components in parts by weight: 37.5-56.25 parts of chicken manure, 0-43.75 parts of cow manure and 0-43.75 parts of sheep manure.
Preferably, the microbial agent comprises one or more of bacillus subtilis, aspergillus oryzae and myceliophthora thermophila.
Preferably, the composting fermentation comprises the step of establishing a strip pile body with the length of 10-50 m and the height of 0.8-1.5 m for fermentation.
Preferably, the mass volume ratio of the decomposed product of the livestock and poultry manure to the water is 1:10 g/mL.
Preferably, the shaking time is 2 hours, and the centrifugation condition is 8000 rpm for 5 minutes.
Preferably, the filtration is performed using a 0.45 μm microfiltration membrane.
The invention also provides a DOM extract obtained by the extraction method in the technical scheme.
The invention provides a method for quickly heating and decomposing livestock and poultry manure and extracting DOM (document object model) based on microbial regulation. The method is simple to operate, the livestock and poultry manure is subjected to resource treatment, and large-scale production can be realized; and moreover, the heap body added with the microbial inoculum has the advantages of quick temperature rise, capability of maintaining continuous high-temperature and high-efficiency fermentation, short decomposition period, rich extracted DOM composition and high yield.
Drawings
FIG. 1 shows the fermentation maturity (A) and DOM content (B) of the livestock and poultry manure provided by the present invention.
Detailed Description
The invention provides a method for quickly heating and decomposing livestock and poultry manure and extracting DOM (soluble organic matter) based on microbial regulation, which comprises the following steps:
1) mixing livestock and poultry manure, mushroom residues and a microbial agent to obtain a premix; the addition amount of the microbial agent is 10% of the total mass of the livestock and poultry manure and the mushroom residue;
2) carrying out composting fermentation on the premix, wherein the highest fermentation temperature of the composting fermentation is controlled to be 60-70 ℃, and stirring is carried out every 3 days;
3) when the germination index of the stacked seeds is more than 70 percent, a livestock and poultry manure decomposed product is obtained;
4) and mixing the livestock and poultry manure decomposed product with water, shaking, centrifuging, taking supernatant, and filtering to obtain a DOM extract.
The method comprises the steps of mixing livestock and poultry manure, mushroom residues and a microbial agent to obtain a premix; the addition amount of the microbial agent is 10% of the total mass of the livestock and poultry manure and the mushroom residue. In the present invention, the mass ratio of the livestock manure to the mushroom dregs is preferably 4: 1. In the present invention, the livestock manure preferably includes one or more of chicken manure, sheep manure, and cow manure. In the present invention, the livestock manure more preferably comprises the following components in parts by weight: 37.5-56.25 parts of chicken manure, 0-43.75 parts of cow manure and 0-43.75 parts of sheep manure. In the invention, the mass ratio of the chicken manure, the sheep manure and the cattle manure in the livestock manure is preferably 3:3: 2. In the present invention, the microbial agent preferably comprises one or more of bacillus subtilis, aspergillus oryzae and myceliophthora thermophila. In the invention, the composting fermentation preferably comprises the step of establishing a windrow pile body with the length of 10-50 m and the height of 0.8-1.5 m for fermentation. The composting of the invention is preferably tank composting, i.e. the invention preferably employs tank fermentation to obtain high value extracts. More preferably, the invention is preferably used to create a stack of stacks, the length of the stack being more preferably 30m and the height of the stack being more preferably 1 m.
After the premix is obtained, the premix is subjected to composting fermentation, the maximum fermentation temperature of the composting fermentation is controlled to be 60-70 ℃, and the mixture is turned and stirred every 3 days. The invention preferably uses the turner to turn and mix (turn and stir) for one time.
When the germination index of the stacked seeds is more than 70 percent, the livestock and poultry manure decomposed product is obtained. The germination index of the seeds is preferably detected by NY/T525-2021 according to a standard.
After the livestock and poultry manure rotten product is obtained, the livestock and poultry manure rotten product is mixed with water, the mixture is shaken and then centrifuged, supernatant liquid is taken, and filtering is carried out, so as to obtain a DOM extract. In the present invention, the mass-to-volume ratio of the animal manure decomposed product to water is preferably 1:10 g/mL. In the present invention, the period of shaking is preferably 2 hours, and the centrifugation condition is preferably 8000 rpm for 5 minutes. In the present invention, the filtration is preferably performed using a 0.45 μm microporous filtration membrane.
The invention also provides a DOM extract obtained by the extraction method in the technical scheme. The method can be applied to large-scale treatment of the livestock and poultry manure and production of high-value extracts, and the used raw materials are easy to obtain and high in yield.
The method for rapid temperature rise and decomposition of livestock and poultry manure and DOM extraction based on microbial regulation according to the present invention is further described in detail with reference to the following specific examples, and the technical solutions of the present invention include, but are not limited to, the following examples.
Example 1
A method for rapidly heating and decomposing livestock and poultry manure and extracting DOM (document object model) based on microbial regulation comprises the following specific implementation steps:
(1) building a strip pile body with the length of 30m and the height of 1 m; premixing chicken manure, sheep manure, cow manure and mushroom residues, wherein the addition amount of the microbial agent is 10% of the total mass of the livestock manure and the mushroom residues;
(2) after the materials are mixed, the mixed materials are piled into a stack by a loader for fermentation, the highest fermentation temperature is controlled between 60 and 70 ℃, and the stack is turned and stirred once by a stack turner every 3 days;
(3) on the 20 th day of composting, the Germination Index (GI) of the stacked seeds is more than 70 percent, and the decomposed product of the livestock and poultry manure is obtained.
(4) Mixing the decomposed product with water at a ratio of 1:10(W/V) (namely the mass volume ratio of the decomposed product of the livestock and poultry manure to the deionized water is 1:10g/mL), placing the mixture in a horizontal shaking table, oscillating the mixture for 2 hours, centrifuging the sample at 8000 rpm for 5 minutes, taking supernatant, and filtering the supernatant through a 0.45 mu m microporous filter membrane to obtain a DOM extract.
The raw materials for preparation comprise the following components in parts by weight: 30 parts of chicken manure, 30 parts of sheep manure, 20 parts of cow manure, 20 parts of mushroom residue and 10 parts of microbial agent.
Wherein the microbial agent comprises Bacillus subtilis, Aspergillus oryzae and myceliophthora thermophila.
FIG. 1 is a graph showing the results of fermentation maturity (A) and DOM content (B) of animal manure, in example 1, the animal manure was rapidly matured by regulation and control of exogenous microorganisms, and the maturity requirement (seed germination index GI > 70%) was reached at day 20, and the extracted DOM content reached 1934 mg/L. In contrast, in comparative example 1, no exogenous microorganism was added, the period to achieve maturity was long, and the DOM content was low.
Has the beneficial effects that: the temperature of the pile body added with the microbial inoculum is fast in the fermentation process, compared with the comparative example 1, the time for reaching the fermentation temperature of 60 ℃ is reduced by 13 days, and the continuous high-temperature fermentation of more than 50 ℃ for 26 days can be maintained; the rotten requirement (germination index (GI) > 70%) is achieved on the 20 th day; the DOM content reaches 1934 mg/L.
Comparative example 1
Comparative example 1 is a fermentation heap without microbial agent addition, keeping other operating conditions unchanged. Comparative example 1 reached the maturity requirement (seed Germination Index (GI) > 70%) on day 38; the DOM content is 941 mg/L.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (10)
1. A method for quickly heating and decomposing livestock and poultry manure and extracting DOM (document object model) based on microbial regulation is characterized by comprising the following steps of:
1) mixing livestock and poultry manure, mushroom residues and a microbial agent to obtain a premix; the addition amount of the microbial agent is 10% of the total mass of the livestock and poultry manure and the mushroom residue;
2) carrying out composting fermentation on the premix, wherein the highest fermentation temperature of the composting fermentation is controlled to be 60-70 ℃, and stirring is carried out every 3 days;
3) when the germination index of the stacked seeds is more than 70 percent, a livestock and poultry manure decomposed product is obtained;
4) and mixing the livestock and poultry manure decomposed product with water, shaking, centrifuging, taking supernatant, and filtering to obtain a DOM extract.
2. The method as claimed in claim 1, wherein the mass ratio of the livestock manure to the mushroom dregs is 4: 1.
3. The method as claimed in claim 1, wherein the livestock manure comprises one or more of chicken manure, sheep manure, and cow manure.
4. The method as claimed in claim 3, wherein the livestock manure comprises the following components in parts by weight: 37.5-56.25 parts of chicken manure, 0-43.75 parts of cow manure and 0-43.75 parts of sheep manure.
5. The method of claim 1, wherein the microbial agent comprises one or more of bacillus subtilis, aspergillus oryzae, and myceliophthora thermophila.
6. The method of claim 1, wherein the composting fermentation comprises establishing a windrow pile 10-50 m long and 0.8-1.5 m high for fermentation.
7. The method as claimed in claim 1, wherein the mass-to-volume ratio of the livestock manure rotten product to the water is 1:10 g/mL.
8. The method of claim 1, wherein the shaking time is 2 hours and the centrifugation conditions are 8000 rpm for 5 minutes.
9. The method of claim 1, wherein the filtration is performed using a 0.45 μm microfiltration membrane.
10. A DOM extract obtained by the extraction method according to any one of claims 1 to 10.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210407580.0A CN114560725A (en) | 2022-04-19 | 2022-04-19 | Livestock and poultry manure rapid heating and decomposing and DOM extracting method based on microbial regulation |
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| CN202210407580.0A CN114560725A (en) | 2022-04-19 | 2022-04-19 | Livestock and poultry manure rapid heating and decomposing and DOM extracting method based on microbial regulation |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104774054A (en) * | 2015-04-28 | 2015-07-15 | 永州万喜登农业发展有限公司 | Bio-organic fertilizer |
| CN107266183A (en) * | 2017-07-06 | 2017-10-20 | 重庆市万源禽蛋食品有限公司 | Chicken manure organic fertilizer and preparation method thereof |
| CN111943774A (en) * | 2020-07-14 | 2020-11-17 | 湖北绿道农业发展有限公司 | Functional biological organic fertilizer and preparation method thereof |
| CN112979987A (en) * | 2021-03-03 | 2021-06-18 | 南开大学 | Method for extracting small-molecular organic matters by utilizing organic fertilizer |
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- 2022-04-19 CN CN202210407580.0A patent/CN114560725A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104774054A (en) * | 2015-04-28 | 2015-07-15 | 永州万喜登农业发展有限公司 | Bio-organic fertilizer |
| CN107266183A (en) * | 2017-07-06 | 2017-10-20 | 重庆市万源禽蛋食品有限公司 | Chicken manure organic fertilizer and preparation method thereof |
| CN111943774A (en) * | 2020-07-14 | 2020-11-17 | 湖北绿道农业发展有限公司 | Functional biological organic fertilizer and preparation method thereof |
| CN112979987A (en) * | 2021-03-03 | 2021-06-18 | 南开大学 | Method for extracting small-molecular organic matters by utilizing organic fertilizer |
Non-Patent Citations (1)
| Title |
|---|
| 陈文新: "《土壤和环境微生物学》", 30 September 1990 * |
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Application publication date: 20220531 |