CN114521454A - Method for breeding and seedling raising of illicium simsii - Google Patents

Method for breeding and seedling raising of illicium simsii Download PDF

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Publication number
CN114521454A
CN114521454A CN202210160458.8A CN202210160458A CN114521454A CN 114521454 A CN114521454 A CN 114521454A CN 202210160458 A CN202210160458 A CN 202210160458A CN 114521454 A CN114521454 A CN 114521454A
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cutting
seedbed
cultivation
cuttage
breeding
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曹永慧
陈一锋
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Research Institute of Subtropical Forestry of Chinese Academy of Forestry
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Research Institute of Subtropical Forestry of Chinese Academy of Forestry
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/10Vegetative propagation by means of cuttings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A01G24/12Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A01G24/12Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
    • A01G24/15Calcined rock, e.g. perlite, vermiculite or clay aggregates

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Soil Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention relates to the technical field of plant cultivation, and discloses a method for breeding and seedling raising of illicium crepidatum. The method comprises the following steps: collecting 1-year-old tender branches and semi-lignified branches of fennel fruits, reserving 1-2 half leaves to prepare cutting slips, soaking the cutting slips in GGR solution, and inserting the cutting slips into a cutting seedbed for shading cultivation, wherein the cultivation substrate of the cutting seedbed is orchid soil and perlite, or river sand and yellow core soil. The method for breeding and seedling raising of the common anethole herb is high in cuttage survival rate and rooting rate, simple and convenient to breed, and beneficial to protection, propagation expansion and medicinal resource protection of the common anethole herb.

Description

Method for breeding and seedling raising of illicium simsii
Technical Field
The invention relates to the technical field of plant cultivation, in particular to a method for breeding and seedling raising of illicium crepidatum.
Background
The Thalictrum acutifolium (Illicium lanifolium), also known as shikimic acid, belongs to the Illicium genus of the family Anisodiaceae, is a special Chinese herb, is a national third-level protective plant, can be used as a Chinese traditional medicinal plant for treating diseases such as rheumatic arthritis, traumatic injury, abdominal distension, emesis, traumatic bleeding and the like, and has the effects of easing pain, clearing and activating the channels and collaterals, diminishing inflammation, stopping bleeding and the like. The common fennel fruit oil is produced by industrialized medicines except for folk traditional use, such as injection (collected in Zhejiang Taikang pharmaceutical industry group Limited, listed in ministerial promulgated standards and national traditional Chinese medicine protection varieties) produced by Zhejiang Taikang pharmaceutical industry group, is mainly used for treating rheumatic arthritis, traumatic injury and the like, and has obvious clinical effect, market and social benefit. The shikimic acid which is the main drug effect component of the inula cappa is complex in chemical synthesis and microbial fermentation process and too high in cost, so that the extraction of a plant source becomes an important way. At present, the main source of plant extraction is the wild plant of the illicium genus, and the extraction rate can reach 10%.
The foeniculum trichocarpa has wider ecological range and is distributed in subtropical areas of China. But the water-wet requirement on the habitat is high, the local distribution range is narrow, the water-wet is mostly distributed sporadically, the growth is slow, the fruiting quantity is small, the seed germination rate is low, and the total quantity of the resources is very limited due to the biological characteristics; in addition, the current method is a plunder type utilization method of digging roots and taking skins, so that wild resources are deficient, and the method becomes a bottleneck for the development of medicine enterprises. The problems of wild population of the illicium manthalium, ecological environment protection and reasonable resource development and utilization are attracting increasing attention, and the development of artificial efficient cultivation of excellent germplasm resources becomes an important effective way at present. However, at present, introduction tests and large-area cultivation of the illicium manthalium blume are not developed at home and abroad, only part of the illicium manthalium blume is sparsely cultivated as landscaping plants, and systematic research on wild population ecology and artificial cultivation forcing technology of the illicium manthalium blume is lacked. Aiming at the problems, the cutting propagation seedling raising method of the common anethole is researched and developed by collecting partial germplasm resources of the common anethole, and aims to provide technical support for large-scale development and utilization of the common anethole.
Disclosure of Invention
The invention aims to enrich the existing seedling propagation technology of medicinal illicium manthalium of rare or endangered species and provides a method for promoting the propagation and seedling culture of the illicium manthalium by cutting scions of the illicium manthalium.
In order to solve the technical problems, the invention provides the following technical scheme:
the invention provides a method for breeding and seedling raising of anethole blumea, which comprises the following steps:
collecting 1-year-old tender branches and semi-lignified branches of fennel fruits, reserving 1-2 half leaves to prepare cutting slips, soaking the cutting slips in GGR solution, and inserting the cutting slips into a cutting seedbed for shading cultivation, wherein the cultivation substrate of the cutting seedbed is orchid soil and perlite, or river sand and yellow core soil.
Preferably, the branches in the invention are spring shoots or autumn shoots collected in spring.
Preferably, the length of the cutting is 8-10 cm.
Preferably, one end of the cutting slip is cut into a horseshoe-shaped plane.
Preferably, the row spacing of the cuttage plants is 8-12 cm.
Preferably, the mass concentration of the GGR solution is 0.007 to 0.0125 percent.
Preferably, the soaking time is 8-16 h.
Preferably, the cuttage is carried out in middle and late ten days of spring 4 months.
Preferably, the shading is implemented by adopting 50% -60% shading net.
Preferably, the incubation temperature is 18-25 ℃.
Preferably, the seedlings are transplanted after being cultivated for 110-130 d.
Compared with the prior art, the invention has the following technical effects:
the invention relates to a cutting shoot prepared from 1-year-old tender branches and semi-lignified branches of fennel shapa, which is characterized in that on the basis of reserving 1-2 half leaves, the cutting shoot is soaked in GGR solution and then inserted into a cutting seedbed for shade cultivation, and cultivation substrates of the cutting seedbed are selected from orchid soil and perlite or river sand and yellow soil. The propagation survival rate of the method for propagating and raising seedlings of the foeniculum vulgare is over 84 percent, and the rooting rate of the cutting slips is over 72 percent.
The cuttage breeding method is simple and easy to realize, the seedbed is sufficient in nutrition, no additional fertilizer is needed after transplanting, breeding is convenient, the protection and propagation expansion and economic value of the method are improved, and the method is favorable for further promoting the protection of medicinal resources of the common anethole herb and improving the development and utilization market prospect.
Detailed Description
The invention provides a method for breeding and seedling raising of Thalictrum foeniculum Hance, which comprises the following steps: collecting 1-year-old tender branches and semi-lignified branches of fennel fruits, reserving 1-2 half leaves to prepare cutting slips, soaking the cutting slips in GGR solution, and inserting the cutting slips into a cutting seedbed for shading cultivation, wherein the cultivation substrate of the cutting seedbed is orchid soil and perlite, or river sand and yellow core soil. By adopting the method disclosed by the invention to propagate and grow seedlings of the fennel fruits of the capejasmine, the propagation survival rate reaches over 84%, and the rooting rate of the cutting slips is over 72%.
In the invention, the branches of the common anethole are preferably collected in spring, 1 year-old tender branches and semi-lignified branches are selected to prepare cuttings, and spring tips or autumn tips can be selected. Preferably, the cutting slips are 8-10cm long from top to bottom, and 1-2 half leaves are reserved. One end of the cutting slip is cut into a horseshoe-shaped plane, so that the cutting slip is convenient to soak and cut.
In the invention, the GGR is a non-hormone green plant growth regulator, is positively correlated with the synthesis of plant endogenous hormones, endogenous polyamines and phenolic compounds and the improvement of the activity of certain metabolism-related enzymes, can influence the absorption and metabolism of plant nutrient elements, and becomes a novel physiologically active substance for regulating the growth and development of plants, establishing organ morphologies and improving the yield. In the present invention, the source of GGR is not particularly limited, and commercially available products may be used. The invention uses GGR solution to soak the cutting slips, thereby improving the survival rate and rooting rate of the cutting slips after cutting. In the invention, the mass concentration of the GGR solution is preferably 0.007-0.0125%, and more preferably 0.009-0.011%. The time for soaking the cutting in the GGR solution is preferably 8-16h, and more preferably 11-13 h.
In the invention, cuttage is preferably carried out in the middle and late ten days of 4 months in spring, the temperature at the time is suitable for the survival of the cutting slips, and the cuttage survival rate is improved. The invention preferably sterilizes the cutting slips first and then inserts the sterilized cutting slips into a sterilized cutting seedling bed. The specific disinfection method is not particularly limited in the present invention, and a disinfection method in the conventional art, such as carbendazim sterilization and disinfection, can be adopted. According to the invention, the plant row spacing of the cutting slips is 8-12cm, and the plant row spacing is 9-11 cm.
According to the invention, the substrate of the cutting seedbed is screened, and when the culture substrate is orchid soil and perlite or river sand and yellow soil, the survival rate and rooting rate of the cutting slips are higher, so that the cutting propagation of the fennel fruits of the capejasmine is more facilitated. In the invention, the volume ratio of the orchid soil to the perlite is 1-1.2: 1 to 1.5, more preferably 1: 1; the volume ratio of the river sand to the yellow core soil is 1-1.2: 1 to 1.5, more preferably 1: 1. in the invention, the orchid soil refers to soil special for cultivating orchids, and can be a commercially available product. The orchid soil is mixed with multifunctional leaf mold, bark, charcoal, broken bricks, fern roots and the like, so that the prepared soil is soft, breathable, rich in humus and strong in drainage capacity.
After the cuttage is finished, preferably, 50% -60% of shading net is used for shading cultivation. The preferable cultivation temperature is 18-25 ℃, more preferably 20-22 ℃, and as the provided cultivation temperature is proper, mulching films are not needed to be covered in the cutting process. The seedbed used in the invention has sufficient nutrition, and no additional fertilizer is needed after transplantation. Watering at proper time according to the humidity of the seedbed.
The illicium manthalium cultivated by the method can be transplanted after 110-130 days, the cuttage survival rate and the rooting rate are high, the method is simple, the breeding is convenient, and the protection propagation and the protection of medicinal resources of the illicium manthalium are facilitated.
The technical solutions of the present invention are further described in detail with reference to the following specific examples, which include but are not limited to the following examples.
Example 1
(1) Ear harvesting season: collecting 1-year-old tender branches and semi-lignified spring shoot branches of the common anethole in spring (4 months), and cutting 8-10cm scion from top to bottom;
(2) leaf treatment mode of the scion: each ear of strip keeps 1-2 half leaves, the front end of the strip is cut into a horseshoe-shaped plane by a quick knife and then bundled;
(3) hormone treatment: and (3) putting the bundled ear strips into a pre-treated GGR solution with the concentration of 1g/10kg for soaking for 12 hours.
(4) And (3) cutting season: cutting in spring (middle and last ten days of 4 months) at 20 deg.C;
(5) cutting density: cutting according to the plant row spacing of 10 cm;
(6) selecting a matrix: selecting a volume ratio of 1: the orchid soil and the perlite of 1 are used as substrates for seedbed finishing.
(7) Seedbed management: uniformly disinfecting a cutting seedbed;
(8) shading treatment: after cuttage in spring (middle and late 4 months) is finished, shading treatment is carried out by using 50% -60% shading nets, and mulching films are not covered.
(9) And (3) fertilizer and water management: the seedbed is sufficient in nutrition, and no additional fertilizer is needed after transplantation. Watering regularly for 2-3 times per month.
After 45 days of cuttage, the cuttage survival rate is 96.0%, the rooting rate of the cuttage wood is 75%, and the average rooting length is 5.0 cm.
Example 2
(1) Ear harvesting season: collecting 1-year-old twig and semi-lignified spring shoot branch of common fennel leaf in spring (4 months), and cutting 8-10cm of scion from top to bottom;
(2) leaf treatment mode of the scion: each ear of strip keeps 1-2 half leaves, the front end of the strip is cut into a horseshoe-shaped plane by a quick knife and then bundled;
(3) hormone treatment: and (3) putting the bundled ear strips into a pre-treated GGR solution with the concentration of 1g/10kg for soaking for 12 hours.
(4) And (3) cutting season: cutting in spring (middle and last ten days of 4 months) at 23 deg.C;
(5) cutting density: cutting according to the plant row spacing of 11 cm;
(6) selecting a matrix: selecting a volume ratio of 1: 1, river sand and yellow core soil are used as substrates for seedbed finishing.
(7) Seedbed management: uniformly disinfecting a cutting seedbed;
(8) shading treatment: after cuttage in spring (middle and late 4 months) is finished, shading treatment is carried out by using 50% -60% shading nets, and mulching films are not covered.
(9) And (3) fertilizer and water management: the seedbed is sufficient in nutrition, and no additional fertilizer is needed after transplantation. Watering regularly for 2-3 times per month.
After 45 days of cuttage, the cuttage survival rate is 94.0%, the rooting rate of the cuttage wood is 86.2%, and the average rooting length is 8.2 cm.
Example 3
(1) Ear harvesting season: collecting 1 year old twig and semi-lignified autumn branch of Thalictrum latifolia in spring (4 months), and cutting 8-10cm ear from top to bottom;
(2) leaf treatment mode of the scion: each ear of strip keeps 1-2 half leaves, the front end is cut into a horseshoe-shaped plane by a quick knife, and then the horseshoe-shaped plane is bundled;
(3) hormone treatment: and (3) putting the bundled ear strips into a pre-treated GGR solution with the concentration of 1g/11kg for soaking for 12 hours.
(4) And (3) cutting season: cutting in spring (middle and last ten days of 4 months) at 21 deg.C;
(5) cutting density: cutting according to the plant row spacing of 9 cm;
(6) selecting a matrix: selecting a volume ratio of 1: the orchid soil and the perlite of 1 are used as substrates for seedbed finishing.
(7) Seedbed management: uniformly disinfecting a cutting seedbed;
(8) shading treatment: after cuttage in spring (middle and late 4 months) is finished, shading treatment is carried out by using 50% -60% shading nets, and mulching films are not covered.
(9) And (3) fertilizer and water management: the seedbed is sufficient in nutrition, and no additional fertilizer is needed after transplantation. Watering regularly for 2-3 times per month.
After 45 days of cuttage, the cuttage survival rate is 98.0%, the rooting rate of the cuttage wood is 72%, and the average rooting length is 6.8 cm.
Example 4
(1) Ear harvesting season: collecting 1-year tender branches and semi-lignified autumn branches of Thamnolia acutifolia Bunge in spring (4 months), and cutting 8-10cm scion from top to bottom;
(2) leaf treatment mode of the scion: each ear of strip keeps 1-2 half leaves, the front end of the strip is cut into a horseshoe-shaped plane by a quick knife and then bundled;
(3) hormone treatment: and (3) putting the bundled ear strips into a pre-treated GGR solution with the concentration of 1g/8kg for soaking for 12 hours.
(4) And (3) cutting season: cutting in spring (middle and last ten days of 4 months) at 22 deg.C;
(5) cutting density: cutting according to the plant row spacing of 12 cm;
(6) matrix selection: selecting a volume ratio of 1: 1, river sand and yellow core soil are used as substrates for seedbed finishing.
(7) Seedbed management: uniformly disinfecting a cutting seedbed;
(8) shading treatment: after cuttage in spring (middle and late 4 months) is finished, shading treatment is carried out by using 50% -60% shading nets, and mulching films are not covered.
(9) And (3) fertilizer and water management: the seedbed is sufficient in nutrition, and no additional fertilizer is needed after transplantation. Watering regularly for 2-3 times per month.
After 45 days of cuttage, the cuttage survival rate is 92.0%, the rooting rate of the cuttage wood is 80%, and the average rooting length is 5.9 cm.
Comparative example 1
The same procedure as in example 1 was repeated, except that the volume ratio of the seedbed substrate was 1: 1 loess and orchid soil.
After 45 days of cuttage, the cuttage survival rate is 85.0%, the rooting rate of the cuttage wood is 69%, and the average rooting length is 4.8 cm.
Comparative example 2
The same procedure as in example 1 was repeated, except that the volume ratio of the seedbed substrate was 1: 1 loess and perlite.
After 45 days of cuttage, the cuttage survival rate is 82.0%, the rooting rate of the cuttage wood is 70.3%, and the average rooting length is 4.3 cm.
Comparative example 3
The same procedure as in example 1 was conducted except that orchid soil was used as the bed substrate.
After 45 days of cuttage, the cuttage survival rate is 84.0%, the rooting rate of the cuttage wood is 64%, and the average rooting length is 4.7 cm.
Comparative example 4
The same procedure as in example 1 was repeated, except that river sand was used as the bed substrate.
After 45 days of cuttage, the cuttage survival rate is 82.0%, the rooting rate of the cuttage wood is 62%, and the average rooting length is 4.7 cm.
Comparative example 5
The same procedure as in example 1 was repeated, except that the GGR solution concentration was 1g/3 kg.
After 45 days of cuttage, the cuttage survival rate is 84.0%, the rooting rate of the cuttage wood is 66%, and the average rooting length is 4.7 cm.
Comparative example 5
The same procedure as in example 1 was followed, except that the GGR solution was replaced with ABT solution at a concentration of 1g/10 kg.
After 45 days of cuttage, the cuttage survival rate is 82.0%, the rooting rate of the cuttage wood is 62%, and the average rooting length is 4.5 cm.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims (10)

1. A method for breeding and raising seedlings of Thalictrum foeniculum Hance is characterized by comprising the following steps:
collecting 1-year-old tender branches and semi-lignified branches of fennel fruits, reserving 1-2 half leaves to prepare cutting slips, soaking the cutting slips in GGR solution, and inserting the cutting slips into a cutting seedbed for shading cultivation, wherein the cultivation substrate of the cutting seedbed is orchid soil and perlite, or river sand and yellow core soil.
2. The method according to claim 1, wherein the shoots are spring shoots or autumn shoots harvested in spring.
3. The method of claim 1, wherein the cutting is 8-10cm in length.
4. The method according to claim 1, wherein the GGR solution has a mass concentration of 0.007-0.0125%.
5. The method of claim 1, wherein the soaking time is 8-16 hours.
6. The method of claim 1, wherein the cutting is performed in mid to late spring 4 months.
7. The method according to claim 1, wherein the row spacing of the cuttings is 8-12 cm.
8. The method of claim 1, wherein the shade is a 50% -60% shade net.
9. The method according to claim 1, wherein the incubation temperature is 18-25 ℃.
10. The method of claim 1, wherein the transplanting is performed after 110-130 days of cultivation.
CN202210160458.8A 2022-02-22 2022-02-22 Method for breeding and seedling raising of illicium simsii Pending CN114521454A (en)

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Citations (4)

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Publication number Priority date Publication date Assignee Title
JPH1175534A (en) * 1997-09-10 1999-03-23 Sumitomo Forestry Co Ltd Nursery stock production system and method
CN102282995A (en) * 2011-06-27 2011-12-21 江苏省中国科学院植物研究所 Cold-resistant excellent lance-leaved red anise strain cutting propagation technology
CN102986441A (en) * 2012-12-17 2013-03-27 苏州大学 Cutting propagation method for ornamental plant star anise with lanceolate leaves
CN107135925A (en) * 2017-06-06 2017-09-08 上海市农业科学院 A kind of mating system of Japanese thick grass seedling

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH1175534A (en) * 1997-09-10 1999-03-23 Sumitomo Forestry Co Ltd Nursery stock production system and method
CN102282995A (en) * 2011-06-27 2011-12-21 江苏省中国科学院植物研究所 Cold-resistant excellent lance-leaved red anise strain cutting propagation technology
CN102986441A (en) * 2012-12-17 2013-03-27 苏州大学 Cutting propagation method for ornamental plant star anise with lanceolate leaves
CN107135925A (en) * 2017-06-06 2017-09-08 上海市农业科学院 A kind of mating system of Japanese thick grass seedling

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
郑成忠等: "优良林下药用观赏树种——披针叶茴香", 《林业调查规划》 *
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