CN114392215A - Composition containing plant callus extract and application thereof - Google Patents

Composition containing plant callus extract and application thereof Download PDF

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Publication number
CN114392215A
CN114392215A CN202111639171.5A CN202111639171A CN114392215A CN 114392215 A CN114392215 A CN 114392215A CN 202111639171 A CN202111639171 A CN 202111639171A CN 114392215 A CN114392215 A CN 114392215A
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Prior art keywords
extract
callus
composition
antioxidant
composition according
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Inventor
周守山
戴穏平
林晓
吴锐
黄怡
招浩燕
曾裕华
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Zhuhai Jintai Biotechnology Co ltd
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Zhuhai Jintai Biotechnology Co ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59—Mixtures
    • A61K2800/592—Mixtures of compounds complementing their respective functions
    • A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dermatology (AREA)
  • Gerontology & Geriatric Medicine (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Cosmetics (AREA)

Abstract

The invention belongs to the technical field of natural functional components, and discloses a composition containing a plant callus extract and application thereof. The composition containing the plant callus extract comprises the following components: antioxidant, Mirabilis jalapa extract and Barlery chicory leaf extract; the antioxidant comprises at least one of extract of callus of Leontopodium alpinum and filtrate of callus culture of Cress maritima. The composition generates a certain synergistic effect by matching the plant callus extracts with other components, and obtains remarkable skin antioxidation and anti-aging effects.

Description

Composition containing plant callus extract and application thereof
Technical Field
The invention belongs to the technical field of natural functional components, and particularly relates to a composition containing a plant callus extract and application thereof.
Background
The callus is a tissue which is newly generated on the surface of a wound after a part of a plant body is stimulated by the wound. Generally, tissue in a plant, which is composed of parenchyma cells and does not form a specific structure, is usually present in a wound of the plant. Modern researches find that plant callus contains various active functional components, and the plant callus has better acceptance and application in the fields of medicines, cosmetics and the like due to better safety and reliability. For example, the tobacco callus extract has the effects of resisting acne and treating glaucoma, the saussurea involucrate callus extract has the effect of resisting tumor, the xanthium sibiricum callus extract has the effect of inhibiting bacteria, and the rhodiola sachalinensis callus has the effect of resisting oxidation.
However, the research degree of the callus extracts is limited at present, and no related report that the callus extracts are combined with other components to play a synergistic effect exists. Therefore, the present invention is intended to provide a composition containing a plant callus extract and having superior performance.
Disclosure of Invention
The present invention is directed to solving at least one of the problems of the prior art described above. Therefore, the invention provides a composition containing plant callus extracts and application thereof. The composition generates a certain synergistic effect by matching the plant callus extracts with other components, and obtains remarkable skin antioxidation and anti-aging effects.
The invention provides a composition containing plant callus extracts, which comprises the following components:
antioxidant, Mirabilis jalapa extract and Barlery chicory leaf extract; the antioxidant comprises at least one of extract of callus of Leontopodium alpinum and filtrate of callus culture of Cress maritima.
The invention takes at least one of the calli extract of the alpine edelweiss and the callus culture filtrate of the coastal cress as the main antioxidant component, and finds that the extract is matched with the Baerbia sinensis leaf extract which is traditionally used as an emollient and the mirabilis jalapa extract which is used as an antibacterial and anti-inflammatory agent, so that the obtained composition has more excellent antioxidant and anti-aging skin care performances.
Preferably, the antioxidant is a Leontopodium alpinum callus extract and a Cress maritima callus culture filtrate.
Preferably, the composition comprises the following components in parts by weight:
Figure BDA0003441302890000021
preferably, the preparation method of the alpine edelweiss callus extract comprises the following steps:
(1) taking leaves of the alpine edelweiss as explants for inducing callus, and inducing by using an MS basal medium containing 0.2-0.3mg/L NAA and 0.5-0.8 mg/L2, 4-D;
(2) and carrying out reflux extraction and concentration on the callus obtained by induction by using a solvent to obtain the alpine edelweiss callus extract.
Wherein, 2,4-D (2, 4-dichlorophenoxyacetic acid) and NAA (naphthylacetic acid) are plant growth regulators and play a role in inducing and generating callus. The invention discovers that the leaf of the edelweiss is used as an explant, and the culture medium with the composition is used for inducing, so that the obtained edelweiss callus extract has better antioxidant performance than a commercially available product.
More preferably, the solvent in step (2) is selected from ethanol or acetone.
The invention also provides a preparation method of the composition, which comprises the following steps:
mixing the antioxidant, Mirabilis jalapa extract and Barlery chive leaf extract to obtain the composition.
The invention also provides the application of the composition in preparing cosmetics.
The invention also provides anti-aging essence which comprises the composition.
Preferably, the amount of the composition in the anti-aging essence is 1.5-3.5 wt%.
Preferably, the anti-aging essence further comprises at least one of a humectant, a whitening component, an emollient, a thickener or water.
Compared with the prior art, the invention has the following beneficial effects:
according to the invention, specific plant callus extracts (alpine edelweiss callus extract and coastal cress callus culture filtrate) with antioxidant effects are matched with Barr dried chicory leaf extract (emollient) and Mirabilis jalapa extract (antibacterial and anti-inflammatory agent), and the antioxidant and anti-aging effects of the prepared composition are obviously improved. In addition, compared with the commercially available alpine leontopodium nepalense callus extract, the alpine leontopodium nepalense callus extract prepared by the preparation method provided by the invention has better performance and higher antioxidant activity.
Detailed Description
In order to make the technical solutions of the present invention more apparent to those skilled in the art, the following examples are given for illustration. It should be noted that the following examples are only preferred embodiments of the present invention, and the claimed protection scope is not limited thereto, and any modification, substitution, combination made without departing from the spirit and principle of the present invention are included in the protection scope of the present invention.
The starting materials, reagents or apparatuses used in the following examples are conventionally commercially available or can be obtained by conventionally known methods, unless otherwise specified.
Example 1
This example provides a composition comprising plant callus extract, the composition comprising the following components in parts by weight:
Figure BDA0003441302890000031
wherein, the alpine edelweiss callus extract is purchased from Tianjin Aisaibo biotechnology limited; the celery coastal callus culture filtrate is produced by French Biotech Marine company; mirabilis jalapa extract was obtained from West Anoushuo Biotech Co., Ltd; baker dried chicory leaf extract was purchased from Croda (Heda).
The preparation method of the composition in the embodiment comprises the following steps:
mixing the extract of Leontopodium alpinum callus, the filtrate of the callus culture of Cress maritima, Mirabilis jalapa extract and the extract of leaf of Borax siccus, and dispersing at 80r/min for about 3min to obtain the composition.
Example 2
This example provides a composition comprising plant callus extract, the composition comprising the following components in parts by weight:
Figure BDA0003441302890000032
wherein the celery coastal callus culture filtrate is produced by French Biotech Marine company; mirabilis jalapa extract was obtained from West Anoushuo Biotech Co., Ltd; baker dried chicory leaf extract was purchased from Croda (Heda).
The alpine edelweiss callus extract is prepared by self, and the specific preparation method comprises the following steps:
(1) taking leaves of the alpine edelweiss as explants for inducing callus, and inducing by using an MS basal medium containing 0.2mg/L NAA and 0.5mg/L2, 4-D;
(2) and (3) preparing the callus obtained by induction into a dry product, performing reflux extraction for 3 times by using 75% ethanol, combining the refluxed liquid medicine, and performing reduced pressure rotary evaporation and concentration to obtain the alpine edelweiss callus extract.
The preparation method of the composition in the embodiment comprises the following steps:
mixing the extract of Leontopodium alpinum callus, the filtrate of the callus culture of Cress maritima, Mirabilis jalapa extract and the extract of leaf of Borax siccus, and dispersing at 80r/min for about 3min to obtain the composition.
Example 3
The embodiment provides an anti-aging essence which comprises the following components in percentage by weight: the composition comprises 3% of the composition in example 2, 5% of sodium hyaluronate, 8% of glycerin, 4% of butanediol, 3% of beta-arbutin, 2% of vitamin C and the balance of water.
Comparative example 1
The comparative example provides a composition comprising plant callus extract, the composition comprising the following components in parts by weight:
6 parts of alpine edelweiss callus extract,
3 parts of a celery coastal callus culture filtrate.
Wherein, the alpine edelweiss callus extract is purchased from Tianjin Aisaibo biotechnology limited; the celery maritime callus culture filtrate is produced by French Biotech Marine company.
The preparation method of the composition in the comparative example comprises the following steps:
mixing the extract of callus of Hibiscus alpinus with the culture filtrate of callus of Oenanthe javanica, and dispersing at 80r/min for about 3min to obtain the composition.
Comparative example 2
The comparative example provides a composition comprising plant callus extract, the composition comprising the following components in parts by weight:
6 parts of alpine edelweiss callus extract,
3 parts of a celery coastal callus culture filtrate,
8 parts of mirabilis jalapa extract.
Wherein, the alpine edelweiss callus extract is purchased from Tianjin Aisaibo biotechnology limited; the celery coastal callus culture filtrate is produced by French Biotech Marine company; mirabilis jalapa extract was purchased from Siemens' excelsior Biotech, Inc.
The preparation method of the composition in the comparative example comprises the following steps:
mixing the extract of callus of Hibiscus alpinus, the filtrate of callus culture of Cress japonicas and the extract of Mirabilis jalapa, and dispersing at 80r/min for about 3min to obtain the composition.
Comparative example 3
The comparative example provides a composition comprising plant callus extract, the composition comprising the following components in parts by weight:
6 parts of alpine edelweiss callus extract,
3 parts of a celery coastal callus culture filtrate,
8 parts of Baer dried chicory leaf extract.
Wherein, the alpine edelweiss callus extract is purchased from Tianjin Aisaibo biotechnology limited; the celery coastal callus culture filtrate is produced by French Biotech Marine company; baker dried chicory leaf extract was purchased from Croda (Heda).
The preparation method of the composition in the comparative example comprises the following steps:
mixing the extract of Leontopodium alpinum callus, the filtrate of Plectranthus maritima callus culture and the extract of Larix arvensis leaf, and dispersing at 80r/min for about 3min to obtain the composition.
Product effectiveness testing
1. Acute skin irritation test
5 SPF-level white rabbits (purchased from the center of Sichuan university) were selected as test animals, and the hairs on the backs of the test animals were cut off to a range of 3 cm. times.3 cm. 0.5g of each of the compositions prepared in examples 1 to 2 and comparative examples 1 to 3 was applied to the unhaired skin of the test animal and fixed with a non-irritating adhesive tape for 2 hours. The test composition was removed from the skin of the test animal and the skin reaction was continuously observed at the site of contact of the test animal with the composition. The test results showed that no erythema or redness appeared on the skin of each test animal after 72 hours, and it was preliminarily judged that the compositions prepared in examples 1 to 2 and comparative examples 1 to 3 were non-irritating under the test conditions, according to the skin irritation level.
2. Anti-oxidation, anti-aging test
In the art, the evaluation of the anti-aging performance is generally characterized by the DPPH radical scavenging ability test, which is based on the following principle: 1, 1-diphenyl-2-trinitrophenylhydrazine (DPPH) is a stable nitrogen center organic free radical, and an alcoholic solution of the DPPH free radical has purple characteristics because the DPPH free radical has a single electron and has strong absorption at 517 nm. When the free radical scavenger exists, the absorption of the free radical scavenger disappears gradually due to the pairing with a single electron of the free radical scavenger, and the fading degree of the free radical scavenger and the number of the electrons received by the free radical scavenger form a quantitative relation, so that a spectrophotometer can be used for carrying out rapid quantitative analysis to detect the free radical scavenging condition, thereby evaluating the anti-aging capability of the sample. The capacity is expressed by clearance rate, and the larger the clearance rate is, the stronger the antioxidation and anti-aging capacity is.
The specific test method comprises the following steps:
1. accurately weighing 20mg of DPPH, and metering the volume of the DPPH to a volumetric flask of 250mL by using absolute ethyl alcohol to serve as a DPPH standard reagent;
2. respectively dissolving the compositions in the examples 1-2 and the comparative examples 1-3 in absolute ethyl alcohol, and setting to obtain corresponding samples to be detected (the samples to be detected are provided with a plurality of concentration gradients);
3. a blank control group (2mL of DPPH standard reagent +1mL of absolute ethanol) and a test group (2mL of DPPH standard reagent +1mL of sample to be tested) were set.
4. After 10min of reaction at room temperature, the absorbance value at 517nm was measured and the clearance was calculated according to the following formula:
clearance (%) - (1-A/A)0)×100％。
Wherein A is0: the absorbance value of the blank control group at 517 nm;
a: and the absorbance value of the sample to be detected at 517 nm.
To eliminate DPPH free radical by 50% active concentration (IC)50) The compositions in examples 1-2 and comparative examples 1-3 were characterized by their radical scavenging efficacy, and the specific test results are shown in table 1.
TABLE 1 measurement results of anti-DPPH radical activity
Figure BDA0003441302890000061
The results of the tests in table 1 show that: compared with the comparative example 1, the composition of the comparative example 3 also comprises the Bals dried chicory leaf extract, but because the Bals dried chicory leaf extract does not have obvious antioxidant and anti-aging properties, the Bals dried chicory leaf extract of the comparative example 3 actually dilutes the alpine leontopodium callus extract and the coastal cress callus culture filtrate, so that the antioxidant and anti-aging properties of the composition are reduced. Compared with the comparative example 1, the composition of the comparative example 2 also comprises the mirabilis jalapa extract, and the mirabilis jalapa extract also has certain antioxidant performance, or can promote the antioxidant performance of the alpine leontopodium callus extract and the coastal cress callus culture filtrate, so that the antioxidant performance of the comparative example 2 is slightly better than that of the comparative example 1. According to the test results of the example 1, the common effect of the mirabilis jalapa extract and the barbary dried chicory moss leaf extract is that the antioxidant and anti-aging effects of the alpine leontopodium calli callus extract and the coastal celery callus culture filtrate are remarkably improved. Comparing example 1 with example 2, it can be seen that the extract of callus of leontopodium alpinum prepared by the method of the present invention has better antioxidant property than commercially available extract of callus of leontopodium alpinum.
The present invention is not limited to the above-described embodiments, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art. Furthermore, the embodiments and features of the embodiments of the present application may be combined with each other without conflict.

Claims (10)

1. A composition comprising a plant callus extract, comprising the following components:
antioxidant, Mirabilis jalapa extract and Barlery chicory leaf extract; the antioxidant comprises at least one of extract of callus of Leontopodium alpinum and filtrate of callus culture of Cress maritima.
2. The composition of claim 1, wherein the antioxidant is an extract of alpine edelweiss callus and a celery maritime callus culture filtrate.
3. The composition according to claim 2, characterized in that it comprises the following components in parts by weight:
Figure FDA0003441302880000011
4. the composition according to any one of claims 1 to 3, wherein the extract of the calli of alpine edelweiss is prepared by:
(1) taking leaves of the alpine edelweiss as explants for inducing callus, and inducing by using an MS basal medium containing 0.2-0.3mg/L NAA and 0.5-0.8 mg/L2, 4-D;
(2) and carrying out reflux extraction and concentration on the callus obtained by induction by using a solvent to obtain the alpine edelweiss callus extract.
5. The composition of claim 4, wherein the solvent in step (2) is selected from ethanol or acetone.
6. A process for the preparation of a composition according to any one of claims 1 to 5, characterized in that it comprises the following steps:
mixing the antioxidant, Mirabilis jalapa extract and Barlery chive leaf extract to obtain the composition.
7. Use of a composition according to any one of claims 1 to 5 for the preparation of a cosmetic product.
8. An anti-aging essence comprising the composition according to any one of claims 1 to 5.
9. The anti-aging essence of claim 8, wherein the composition is used in an amount of 1.5 to 3.5 wt% in the anti-aging essence.
10. The anti-aging essence according to claim 8 or 9, further comprising at least one of a moisturizing agent, a whitening component, an emollient, a thickener, or water.
CN202111639171.5A 2021-12-29 2021-12-29 Composition containing plant callus extract and application thereof Pending CN114392215A (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109758402A (en) * 2019-03-29 2019-05-17 成都科奥生物工程有限公司 It is a kind of it is living can life stoste and preparation method thereof
CN110731919A (en) * 2018-07-20 2020-01-31 伽蓝（集团）股份有限公司 Application of Mirabilis jalapa extracts and skin external preparation containing the same
CN112941010A (en) * 2021-01-30 2021-06-11 湖北嘉弘福科技有限责任公司 Plant callus efficacy filtrate and exosome extraction method

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110731919A (en) * 2018-07-20 2020-01-31 伽蓝（集团）股份有限公司 Application of Mirabilis jalapa extracts and skin external preparation containing the same
CN109758402A (en) * 2019-03-29 2019-05-17 成都科奥生物工程有限公司 It is a kind of it is living can life stoste and preparation method thereof
CN112941010A (en) * 2021-01-30 2021-06-11 湖北嘉弘福科技有限责任公司 Plant callus efficacy filtrate and exosome extraction method

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