CN114391431B - Co-cultivation method of parasitic plant and host plant - Google Patents
Co-cultivation method of parasitic plant and host plant Download PDFInfo
- Publication number
- CN114391431B CN114391431B CN202210028592.2A CN202210028592A CN114391431B CN 114391431 B CN114391431 B CN 114391431B CN 202210028592 A CN202210028592 A CN 202210028592A CN 114391431 B CN114391431 B CN 114391431B
- Authority
- CN
- China
- Prior art keywords
- parasitic
- polyethylene bag
- solution
- plant seeds
- parasitic plant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/60—Flowers; Ornamental plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Soil Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a co-cultivation method of parasitic plants and host plants. The method comprises the following steps: 1) Placing the host plant seedlings into a polyethylene bag containing glass fiber filter paper, and adding Hoagland solution for culturing; 2) Sterilizing the parasitic plant seeds, and performing preculture after air drying; 3) Adding germination stimulus into the pre-cultured parasitic plant seeds, and culturing in a dark place; 4) Removing Hoagland nutrient solution in the polyethylene bag, picking up parasitic plant seeds with forceps under a split microscope, and arranging the parasitic plant seeds along the root system of a host plant; 5) The polyethylene bag is vertically placed and cultivated in a dark place, and a proper amount of low-concentration Hoagland solution is added every day during the cultivation period, so that the glass fiber filter paper is slightly wet, and no solution is accumulated in the polyethylene bag. By adopting the co-cultivation method, the interaction condition of the parasitic plants and the host plants at different stages can be accurately researched under the laboratory condition, and the diffusion of the parasitic plants can be avoided.
Description
Technical Field
The invention relates to the technical field of plant parasitism, in particular to a co-cultivation method of parasitic plants and host plants.
Background
In nature, the vast majority of seed plants are autotrophic. However, a few seed plants become parasitic due to lack of sufficient chlorophyll or due to degeneration of the root system or leaves, called parasitic plants. The parasitic plants can obtain all or most of water and nutrients needed by green plants, so that the parasitic plants can survive. The parasitic plants are of various kinds and mainly classified into Orobanchaceae, tulipae, moraceae, scrophulariaceae, etc. Parasitic plants are mostly parasitic on mountain plants and trees, some of which are valuable medicinal plants; while a few parasitic seed plants are parasitic on crops, causing a major hazard in agricultural production, such as broomrape and the like.
Scientists have done much work to study the chemistry, biology, and general control methods of parasitic plants. However, parasitic plants often produce hundreds of thousands of seeds that survive in the soil for decades, creating a seed pool that is nearly impossible to completely eradicate. Once parasitic plants appear in the field, their hazards will increase year by year. The parasitic plant generation area in areas such as Huzhenshengmu village, guyang county and Daoceangqi in Jiuyuan Haotou city in Mongolian in 2011 is approximately 1.33 ten thousand hm < 2 >, the parasitic rate reaches 10-35%, the parasitic rate of more serious land areas on the coast of the yellow river reaches 75%, and the parasitic rate in areas reaches 100% by 2012, and the yield of host plants is reduced by about 35%. Thus, studies of parasitic plants in the field can create biological invasion locally, causing large area yield loss and even death of the host plant. In order to avoid adverse effects on the local ecological environment during the research process, while at the same time maximally simulating the interaction process of the parasitic plant with the host plant, a co-cultivation method for studying the parasitic plant interaction with the host plant under laboratory conditions, and which can prevent the parasitic plant from spreading, is needed.
The life history of root parasitic plants mainly includes the following important stages: pre-culturing seeds, germinating seeds, attaching host root systems and forming attachment cells, penetrating host root system tissues, forming a sucker and connecting with a host conduit, forming tumors, growing stems and unearthing, flowering and forming seeds. The absorber formation is preceded by an autotrophic phase and is followed by a parasitic phase. Resistance of a host plant to a parasitic plant can be divided into three stages: a parasitic plant pre-attachment phase (Before attachment) comprising parasitic plant seed germination and aspirator induction; a parasitic Establishment period (Establishment) which is a process of establishing physical connection between the parasitic plant and the host plant; parasitic build-up later (After establishment). However, since the root parasitic plant is parasitic on the underground part of the host plant, it is difficult to observe the whole parasitic process in real time, and it is unfavorable to study the interaction of the root parasitic plant and the host plant, so that it is necessary to invent a co-cultivation method capable of visually displaying the whole interaction process under the soilless condition.
In summary, in order to study the interaction between the parasitic plant and the host plant, it is important to develop a co-cultivation method that can accurately study the interaction between the parasitic plant and the host plant at different stages under laboratory conditions and avoid the diffusion of the parasitic plant.
Disclosure of Invention
Based on the research on the interaction of root parasitic weeds and host crops, the invention provides a method for researching the co-cultivation of parasitic plants and host plants, which can accurately research the interaction condition of the parasitic plants and the host plants at different stages under laboratory conditions and can avoid the diffusion of the parasitic plants.
The technical scheme adopted by the invention is as follows:
a method of co-culturing a parasitic plant with a host plant, comprising the steps of:
(1) Placing the host plant seedlings into a polyethylene bag containing glass fiber filter paper, and adding Hoagland solution for culturing;
(2) Sterilizing the parasitic plant seeds, and performing preculture after air drying;
(3) Adding germination stimulus into the pre-cultured parasitic plant seeds, and culturing in a dark place;
(4) Removing Hoagland nutrient solution in the polyethylene bag, picking up parasitic plant seeds with forceps under a split microscope, and arranging the parasitic plant seeds along the root system of a host plant;
(5) The polyethylene bag is vertically placed and cultivated in a dark place, and a proper amount of low-concentration Hoagland solution is added every day during the cultivation period, so that the glass fiber filter paper is slightly wet, and no solution is accumulated in the polyethylene bag.
Preferably, in the step (1), the host plant seedlings are placed in a polyethylene bag containing glass fiber filter paper, and a Hoagland solution is added for culturing, specifically: wetting glass fiber filter paper in a polyethylene bag by using sterile water, and placing a host plant root system cleaned by using the sterile water on the glass fiber filter paper; placing the polyethylene bag horizontally in a closed container, and standing in a high humidity environment overnight; the next day, a proper amount of low-concentration Hoagland solution is added into a polyethylene bag for culture, then the polyethylene bag is vertically placed, and the polyethylene bag is covered by tinfoil paper, so that the root system of a host plant is placed in a dark environment, and a proper amount of low-concentration Hoagland solution is added into the polyethylene bag for culture every day.
Preferably, a proper amount of low-concentration Hoagland solution is added into a polyethylene bag for culture the next day, and the method specifically comprises the following steps: the next day 6ml of 1/8 strength Hoagland solution was added to the polyethylene bag and incubated for 1 day, and the use of low strength Hoagland solution prevented salt stress on the host plants.
Preferably, a proper amount of low-concentration Hoagland solution is added into a polyethylene bag for culture every day, and the method specifically comprises the following steps: 6mL of 1/4 concentration Hoagland solution is added into a polyethylene bag for 10 to 14 days.
Preferably, in the step (2), the parasitic plant seeds are sterilized, air-dried, and then pre-cultured, specifically: sterilizing the parasitic plant seeds sequentially with an ethanol solution and a disinfectant, neutralizing the disinfectant with a hydrochloric acid solution, rinsing the parasitic plant seeds with sterile water, and then thoroughly air-drying the parasitic plant seeds; uniformly scattering the sterilized and air-dried parasitic plant seeds on a culture dish with wet glass fiber filter paper, sealing the culture dish and wrapping the culture dish with tinfoil, so that the parasitic plant seeds are pre-cultured for 6-7 days in dark and damp-heat environments.
Preferably, the ethanol solution is 70% ethanol solution (volume fraction), and the disinfectant is 1% sodium hypochlorite solution (mass fraction) and 2 drops of Silwet or Sylgard surfactant are added; the hydrochloric acid solution is 0.01M hydrochloric acid solution.
Preferably, the treatment time of the ethanol solution is 30-60 seconds; the treatment time of the disinfectant is 4-8 minutes; the treatment time of the hydrochloric acid solution is 4-8 minutes.
Preferably, in the step (3), germination stimulus is added to the parasitic plant seeds after preculture, and the parasitic plant seeds are cultivated in a dark place, specifically: and sucking the pre-cultured parasitic plant seeds with sterile paper towel to remove excessive water, adding GR24 working solution, sealing the culture dish and wrapping with tinfoil paper, so that the parasitic plant seeds are cultured in dark and moist heat environment for 1-2 days.
Preferably, the concentration of the GR24 working solution is 2mg/L.
Preferably, in the step (5), the light-shielding cultivation is to wrap a polyethylene bag with tin paper, so that the root is cultivated in a dark environment; the addition of an appropriate amount of low-concentration Hoagland solution per day was 1mL of 1/4 concentration Hoagland solution per day.
The invention has the beneficial effects that:
in the co-culture method of the parasitic plant and the host plant, the concentration of the Hoagland solution is optimized, the low-concentration Hoagland solution is used for culture, salt stress on the host plant can be prevented, parasitic plant seeds are arranged along the root system of the host plant, the existence of the host root system can be quickly sensed after the parasitic plant seeds germinate, suicide germination is effectively inhibited, on the other hand, the addition amount of the Hoagland solution during co-culture is controlled, the glass fiber filter paper is slightly wet, and the polyethylene bag is free from solution accumulation, so that a slightly dry co-culture environment is kept, concentration gradient is formed on the glass fiber filter paper by the root secretions of the host plant, the parasitic plant is easier to sense the position of the host plant root system, and the parasitic efficiency is improved. The co-cultivation method of the parasitic plant and the host plant can accurately study the interaction condition of the parasitic plant and the host plant at different stages under the laboratory condition, and can avoid the diffusion of the parasitic plant.
Drawings
FIG. 1 is an Arabidopsis seedling in example 1 placed on glass fiber filter paper.
FIG. 2 is a graph of the initiation of normal growth of Egyptian on Arabidopsis roots in example 1.
FIG. 3 is a growth of sunflower broomrape on the sunflower root system in example 2. A, parasitic on sunflower broomrape to sunflower root system after 7 days of co-culture; and B, after 28 days of co-culture, the sunflower broomrape starts to grow normally on the sunflower root system.
FIG. 4 is the growth of the sunflower broomrape of comparative example 1 on the sunflower root system. After 28 days of co-culture, the sunflower broomrape starts to grow normally on the sunflower root system; example 2 was compared with the parasitic plant of comparative example 1 in terms of parasitic rate.
Detailed Description
The following examples are further illustrative of the invention and are not intended to be limiting thereof.
The Hoagland solution in the invention is a commonly used culture solution and can be prepared according to a conventional formula.
Example 1
(1) Sowing Arabidopsis seeds in peat soil for 7 days, and selecting Arabidopsis seedlings with consistent growth vigor for preparing transplanting; preparing glass fiber filter paper with the size of 20cm multiplied by 9cm, sterilizing and placing in a polyethylene bag with the corresponding size; taking out Arabidopsis seedlings from soil, washing with tap water, and placing the Arabidopsis seedlings in sterile water to clean small soil particles attached to roots; wetting glass fiber filter paper in a polyethylene bag by using sterile water, and placing 5 cleaned arabidopsis root systems on the glass fiber filter paper (shown in figure 1); placing the polyethylene bag horizontally in a closed container, and making the Arabidopsis thaliana adapt to a soilless environment in a high humidity environment overnight; 6mL of 1/8 concentration Hoagland solution is added into the polyethylene bag for supplementing nutrition and culturing for 1 day; placing the polyethylene bag vertically, and covering the polyethylene bag with tinfoil to make the root system of Arabidopsis thaliana be placed in dark environment; 6mL of 1/4 strength Hoagland solution was added daily to the polyethylene bag and the parasitic plants were inoculated after 14 days.
(2) Placing the orobanche aegypti seeds in double-layer teabag paper, and sterilizing the teabag paper filled with the orobanche aegypti seeds in 70% ethanol solution for 30 seconds in a fume hood; then placing the tea bag paper into a disinfectant (2 drops of Silwet or Sylgard surfactant are added into 1% sodium hypochlorite solution) for disinfection for 4 minutes, and stirring timely until the Egypt broomrape seeds are changed from black to brown; then placing the tea bag paper in 0.01M hydrochloric acid solution for 4 minutes, stirring at proper time, and neutralizing the disinfectant; then placing the tea bag paper in sterile water for rinsing for 8 minutes, stirring at proper time, and repeating the rinsing step for 4 times, so as to thoroughly rinse the Egyptian seeds; placing the tea bag paper in a fume hood for 12 hours to thoroughly air-dry the Egyptian seeds; placing 2 layers of sterilized glass fiber filter paper in a culture dish, wetting with sterile water, uniformly scattering the sterilized and air-dried Egyptian seeds on the glass fiber filter paper, sealing the culture dish by a Parafilm film, wrapping the culture dish by tinfoil paper, and pre-culturing the Egyptian seeds in a dark and damp-heat environment for 7 days for subsequent co-culture.
(3) Dissolving 10mg of parasitic plant germination stimulator GR24 in 10mL of acetone, and using sterile water to fix the volume to 100mL to form 100mg/L GR24 mother liquor, and placing the mother liquor in a refrigerator at the temperature of minus 20 ℃ for standby; diluting 20 mu L of GR24 mother solution into 1mL of sterile water to form 2mg/L of GR24 working solution; absorbing excessive water from the pre-cultured Egyptian seeds in the step (2) by using a sterile paper towel, adding 2mg/L of GR24 working solution, sealing a culture dish by using a paramilm film, wrapping the culture dish by using tinfoil paper, culturing the Egyptian seeds in a dark and moist heat environment for 1 day, stimulating the Egyptian seeds to germinate, absorbing excessive GR24 solution from the cultured Egyptian seeds by using the sterile paper towel, preparing for inoculation, removing excessive Hoagland nutrient solution in the polyethylene bag, clamping the Egyptian seeds by using forceps under a microscope, arranging the Egyptian seeds along the root system of Arabidopsis (the step is one of keys of parasitic success, can effectively prevent 'suicide germination'), and vertically placing the polyethylene bag, the tinfoil paper is wrapped to make the roots in a dark environment, 1mL of 1/4 concentration Hoagland solution is added every day during the period, so that the glass fiber filter paper is slightly moist, and no solution is accumulated in the polyethylene bag (the stage is one of key steps of successful parasitism, the slightly dry co-culture environment is convenient for forming concentration gradient on the glass fiber filter paper by root secretions of host plants, so that the parasitic plants are easier to sense the positions of the root systems of the host plants, and the parasitic efficiency is improved, but the excessively dry environment can cause the plants to die due to water deficiency, so that the stage is required to be slightly moist by the glass fiber filter paper, and the polyethylene bag is free from solution accumulation), and after 7 days Egyptian strawberries start to parasitize on an Arabidopsis root system, and after 28 days Egyptian strawberries start to grow normally (shown in figure 2).
Example 2
(1) Sowing sunflower seeds in peat soil for 14 days, selecting sunflower seedlings with consistent growth vigor, preparing glass fiber filter paper with the size of 20cm multiplied by 20cm, sterilizing, and placing in a polyethylene bag with the corresponding size; taking out sunflower seedlings from soil, washing with tap water, and then placing the sunflower seedlings in sterile water to clean small soil particles attached to roots; wetting glass fiber filter paper in a polyethylene bag by using sterile water, and then placing 1 sunflower root system cleaned on the glass fiber filter paper; placing the polyethylene bag horizontally in a closed container, and standing overnight in a high humidity environment to enable the sunflower to adapt to a soilless environment; 6mL of 1/8 concentration Hoagland solution is added into the polyethylene bag for supplementing nutrition and culturing for 1 day; placing the polyethylene bag vertically, and covering the polyethylene bag with tinfoil paper to make the root system of sunflower be placed in dark environment; 6mL of 1/4 strength Hoagland solution was added daily to the polyethylene bag and the parasitic plants were inoculated after 14 days.
(2) Placing the sunflower broomrape seeds in double-layer tea bag paper, sterilizing the tea bag paper filled with the sunflower broomrape seeds in 70% ethanol solution for 60 seconds in a fume hood, then placing the tea bag paper in a sterilizing solution (2 drops of Silwet or Sylgard surfactant are added into 1% sodium hypochlorite solution) for sterilizing for 4 minutes, and stirring at proper time until the sunflower broomrape seeds are changed from black to brown; then placing the tea bag paper in 0.01M hydrochloric acid solution for 4 minutes, stirring at proper time, and neutralizing the disinfectant; then placing the tea bag paper in sterile water for rinsing for 8 minutes, stirring at proper time, and repeating the rinsing step for 4 times to thoroughly rinse the sunflower broomrape seeds; placing the tea bag paper in a fume hood for 12 hours to thoroughly air-dry the sunflower broomrape seeds; placing 2 layers of sterilized glass fiber filter paper in a culture dish, wetting with sterile water, uniformly scattering the sterilized and air-dried sunflower broomrape seeds on the glass fiber filter paper, sealing the culture dish by a Parafilm, wrapping the culture dish by tinfoil paper, and pre-culturing the sunflower broomrape seeds in a dark and moist heat environment for 7 days for subsequent co-culture.
(3) Dissolving 10mg of parasitic plant germination stimulator GR24 in 10mL of acetone, and using sterile water to fix the volume to 100mL to form 100mg/L GR24 mother liquor, and placing the mother liquor in a refrigerator at the temperature of minus 20 ℃ for standby; diluting 20 mu L of the GR24 mother solution into 1mL of sterile water to form 2mg/L of GR24 working solution; sucking the pre-cultured sunflower broomrape seeds with sterile paper towel to remove excessive water, adding 2mg/L GR24 working solution, sealing the culture dish with a Parafilm, wrapping with tinfoil paper, culturing the sunflower broomrape seeds in dark and moist heat environment for 1 day, and stimulating the sunflower broomrape seeds to germinate; sucking the cultured sunflower broomrape seeds with sterile paper towel to remove excess GR24 solution, and preparing for inoculation; removing excessive Hoagland nutrient solution in the polyethylene bag, and then clamping the sunflower broomrape seeds by forceps under a split microscope and arranging the seeds along the root system of the sunflower (the step is one of the keys of successful parasitism, and suicide germination can be effectively prevented); then vertically placing the polyethylene bag, and wrapping the polyethylene bag with tinfoil paper to make the root part in a dark environment; during this period 1ml of 1/4 concentration Hoagland solution was added daily to ensure that the glass fiber filter paper was slightly moist and that no solution was accumulated in the polyethylene bag (this stage is one of the key steps for successful parasitism, the slightly dry co-cultivation environment is convenient for the root secretions of the host plants to form a concentration gradient on the glass fiber filter paper, so that the parasitic plants can more easily sense the position of the root systems of the host plants, thereby improving the parasitism efficiency, but too dry environment can cause the plants to die when water deficiency occurs, so this stage should be slightly moist with the glass fiber filter paper and no solution accumulation in the polyethylene bag, and sunflower brooms begin to parasitize on the sunflower root system after 7 days (as shown in FIG. 3A), and sunflower brooms begin to grow normally after 28 days (as shown in FIG. 3B).
Comparative example 1
Steps (1) and (2) are the same as in example 2.
(3) Dissolving 10mg of parasitic plant germination stimulator GR24 in 10mL of acetone, and using sterile water to fix the volume to 100mL to form 100mg/L GR24 mother liquor, and placing the mother liquor in a refrigerator at the temperature of minus 20 ℃ for standby; diluting 20 mu L of the GR24 mother solution into 1mL of sterile water to form 2mg/L of GR24 working solution; sucking the pre-cultured sunflower broomrape seeds with sterile paper towel to remove excessive water, adding 2mg/L GR24 working solution, sealing the culture dish with a Parafilm, wrapping with tinfoil paper, culturing the sunflower broomrape seeds in dark and moist heat environment for 1 day, and stimulating the sunflower broomrape seeds to germinate; sucking the cultured sunflower broomrape seeds with sterile paper towel to remove excess GR24 solution, and preparing for inoculation; removing excessive Hoagland nutrient solution in the polyethylene bag, and then clamping the sunflower broomrape seeds with forceps under a split microscope, and arranging the seeds along the root system of the sunflower; then vertically placing the polyethylene bag, and wrapping the polyethylene bag with tinfoil paper to make the root part in a dark environment; during this period 8ml of 1/4 concentration of hopland solution was added daily and after 28 days sunflower broomrape began to grow normally (as shown in figure 4A), the parasitic plant parasitic rate was significantly reduced in this comparative example compared to example 2 (as shown in figure 4B).
The foregoing is merely a preferred embodiment of the present invention, and it should be noted that the above-mentioned preferred embodiment should not be construed as limiting the invention, and the scope of the invention should be defined by the appended claims. It will be apparent to those skilled in the art that various modifications and adaptations can be made without departing from the spirit and scope of the invention, and such modifications and adaptations are intended to be comprehended within the scope of the invention.
Claims (1)
1. A method of co-culturing a parasitic plant with a host plant, comprising the steps of:
(1) Wetting glass fiber filter paper in a polyethylene bag by using sterile water, and placing a host plant root system cleaned by using the sterile water on the glass fiber filter paper; placing the polyethylene bag horizontally in a closed container overnight; adding 6mL of 1/8 concentration Hoagland solution into a polyethylene bag for culturing for 1 day, then vertically placing the polyethylene bag, covering the polyethylene bag with tinfoil paper, placing the root system of a host plant in a dark environment, and adding 6mL of 1/4 concentration Hoagland solution into the polyethylene bag for culturing for 10-14 days every day;
(2) Sequentially treating the parasitic plant seeds with 70% ethanol solution for 30-60 seconds, treating the parasitic plant seeds with a disinfectant for 4-8 minutes, treating the parasitic plant seeds with 0.01M hydrochloric acid solution for 4-8 minutes to neutralize the disinfectant, rinsing the parasitic plant seeds with sterile water, and then thoroughly air-drying the parasitic plant seeds; uniformly scattering the sterilized and air-dried parasitic plant seeds on a culture dish with wet glass fiber filter paper, sealing the culture dish and wrapping the culture dish with tinfoil, so that the parasitic plant seeds are pre-cultured for 6-7 days in a dark and damp-heat environment; the disinfectant is prepared by adding 2 drops of Silwet or Sylgard surfactant into a sodium hypochlorite solution with the mass fraction of 1%;
(3) Absorbing excessive water from the pre-cultivated parasitic plant seeds by using a sterile paper towel, adding 2mg/L GR24 working solution, sealing a culture dish and wrapping the culture dish by using tinfoil, so that the parasitic plant seeds are cultivated in a dark and moist heat environment for 1-2 days;
(4) Removing Hoagland nutrient solution in the polyethylene bag, picking up parasitic plant seeds with forceps under a split microscope, and arranging the parasitic plant seeds along the root system of a host plant;
(5) The polyethylene bag was placed vertically, wrapped with tin paper, the roots were allowed to incubate in a dark environment, 1mL of 1/4 strength Hoagland solution was added daily during incubation, the glass fiber filter paper was slightly moist, and no solution accumulated in the polyethylene bag.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210028592.2A CN114391431B (en) | 2022-01-11 | 2022-01-11 | Co-cultivation method of parasitic plant and host plant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210028592.2A CN114391431B (en) | 2022-01-11 | 2022-01-11 | Co-cultivation method of parasitic plant and host plant |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114391431A CN114391431A (en) | 2022-04-26 |
| CN114391431B true CN114391431B (en) | 2023-05-12 |
Family
ID=81231819
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210028592.2A Active CN114391431B (en) | 2022-01-11 | 2022-01-11 | Co-cultivation method of parasitic plant and host plant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114391431B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102138457A (en) * | 2011-02-16 | 2011-08-03 | 中国农业大学 | Method for inoculating Cistanche tubulosa by pot culture |
| CN104145683A (en) * | 2014-08-18 | 2014-11-19 | 新疆农业科学院经济作物研究所 | Method for rapidly identifying broomrape resistance level of sunflowers |
| CN106888948A (en) * | 2017-01-17 | 2017-06-27 | 深圳市铁汉生态环境股份有限公司 | A kind of seed propagation method of first hat rattan |
| CN110393129A (en) * | 2019-07-02 | 2019-11-01 | 内蒙古农业大学 | A method of utilizing indoor culture dish Establishing sunflower broomrape parasitism tomato |
| CN110583657A (en) * | 2019-09-12 | 2019-12-20 | 浙江大学 | Method for efficiently collecting and purifying broomrape herb germination stimulators by using aeroponics and solid-phase extraction technology |
| EP3662752A1 (en) * | 2018-12-03 | 2020-06-10 | Institutul National de Cercetare-Dezvoltare Pentru Chimie si Petrochimie-Icehim | Biostimulant for plants obtained from root exudates accumulated in recirculated hydroponic environments |
| CN112425611A (en) * | 2020-11-17 | 2021-03-02 | 新疆农业大学 | Application of artemisia annua extract in stimulating broomrape seed germination |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE139413T1 (en) * | 1991-03-26 | 1996-07-15 | Mogen Int | METHOD FOR ISOLATION AND/OR SAMPLING GENES AND PROMOTORS RELATED TO PLANT-NEMATOD INTERACTIONS USING PLANTS OF THE SEX ARABIDOPSIS |
| WO2006098626A2 (en) * | 2005-03-18 | 2006-09-21 | Plant Research International B.V. | Resistance against parasitic weeds |
| CN101803519A (en) * | 2010-03-26 | 2010-08-18 | 石河子大学 | Cultivation method for inoculating cistanche salsa by using tamarix chinensis |
| US20160021830A1 (en) * | 2013-12-17 | 2016-01-28 | The Penn State Research Foundation | Manipulation of light spectral quality to reduce parasitism by cuscuta and other plant parasites |
| CN104285535B (en) * | 2014-05-19 | 2016-02-24 | 浙江大学 | A kind of method of rapid screening Parasitic Weeds sensitive herbicide |
| CN106134686A (en) * | 2015-04-16 | 2016-11-23 | 宁夏农林科学院 | The method that Herba Cistanches is efficiently parasitic |
| CN106258881A (en) * | 2016-08-11 | 2017-01-04 | 浙江大学 | A kind of co-culture method for studying Parasitic Weeds and host crop interaction |
| CN106508460B (en) * | 2016-12-30 | 2020-07-07 | 内蒙古农业大学 | Method for rapidly identifying broomrape resistance level of sunflower indoors |
-
2022
- 2022-01-11 CN CN202210028592.2A patent/CN114391431B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102138457A (en) * | 2011-02-16 | 2011-08-03 | 中国农业大学 | Method for inoculating Cistanche tubulosa by pot culture |
| CN104145683A (en) * | 2014-08-18 | 2014-11-19 | 新疆农业科学院经济作物研究所 | Method for rapidly identifying broomrape resistance level of sunflowers |
| CN106888948A (en) * | 2017-01-17 | 2017-06-27 | 深圳市铁汉生态环境股份有限公司 | A kind of seed propagation method of first hat rattan |
| EP3662752A1 (en) * | 2018-12-03 | 2020-06-10 | Institutul National de Cercetare-Dezvoltare Pentru Chimie si Petrochimie-Icehim | Biostimulant for plants obtained from root exudates accumulated in recirculated hydroponic environments |
| CN110393129A (en) * | 2019-07-02 | 2019-11-01 | 内蒙古农业大学 | A method of utilizing indoor culture dish Establishing sunflower broomrape parasitism tomato |
| CN110583657A (en) * | 2019-09-12 | 2019-12-20 | 浙江大学 | Method for efficiently collecting and purifying broomrape herb germination stimulators by using aeroponics and solid-phase extraction technology |
| CN112425611A (en) * | 2020-11-17 | 2021-03-02 | 新疆农业大学 | Application of artemisia annua extract in stimulating broomrape seed germination |
Non-Patent Citations (7)
| Title |
|---|
| An improved axenic system for studying pre-infection development of the parasitic plant Orobanche ramosa;Gonzalez-Verdejo, CI等;ANNALS OF BOTANY;第96卷(第06期);第1121-1127页 * |
| The Effect of Virulence and Resistance Mechanisms on the Interactions between Parasitic Plants and Their Hosts;Hu, LY等;INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES;第21卷(第23期);全文 * |
| 中国寄生杂草研究进展;黄建中等;杂草科学(第04期);第8-11页 * |
| 小茴香诱导向日葵列当种子萌发的研究;陈芳洁等;北方园艺(第15期);第5-12页 * |
| 植物根际促生菌作用机制研究进展;康贻军等;应用生态学报;第21卷(第01期);第232-238页 * |
| 水分胁迫对瓜列当、加工番茄发芽和寄生关系的影响;沙洁等;新疆农业科学;第54卷(第04期);第707-714页 * |
| 甘蔗叶片响应褐锈病菌(Puccinia melanocephala)侵染的转录组分析;高小宁等;中国农学通报;第37卷(第24期);第102-109页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114391431A (en) | 2022-04-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103348913B (en) | Rapid propagation method for bletilla striata | |
| CN101822220B (en) | Method for culturing and rapidly propagating stem tip tissue of rare cymbidium goeringii | |
| CN111226533B (en) | Method for germinating mallotus japonicus seeds and seedling culture method | |
| CN101278642A (en) | Method for training tissue culture seedling of tomato | |
| CN106755083B (en) | A kind of pocket orchid Agrobacterium Ovary injection transgenic method | |
| CN105145359A (en) | Tissue culture and rapid propagation method for asparagus filicinus | |
| CN106342512A (en) | Method for improving germination rate of Primula malacoides | |
| CN104081912A (en) | Method for seed germination and seedling growing on wild lycium ruthenicum | |
| CN101702968B (en) | Method for improving germination percentage of seeds of oriental lilies | |
| CN106879275B (en) | Method for rapidly and effectively improving germination rate of sophora tonkinensis seeds | |
| CN103858771A (en) | Maize transgenic tissue culture seedling transplanting method | |
| CN108370808B (en) | Pinus massoniana mycorrhizal seedling raising method | |
| JP7281430B2 (en) | Method for promoting seed germination of difficult-to-germ plants | |
| CN113068560A (en) | Container seedling raising method for ammopiptanthus mongolicus | |
| KR20080070106A (en) | Mass propagation method through in vitro aseptic germination of calanthe spp | |
| CN114391431B (en) | Co-cultivation method of parasitic plant and host plant | |
| CN115152352B (en) | Industrial hemp aseptic seedling breeding method | |
| CN114175978B (en) | Extraction method of celery root secretion and application of celery root secretion in inducing tomatoes to generate anti-bemisia tabaci defense reaction | |
| CN105409513A (en) | Artificial cultivation method of pulsatilla chinensis | |
| CN107691160A (en) | A kind of breeding method of the nontoxic bulb of david lily | |
| CN109588181A (en) | A kind of hill gooseberry's seedling-raising technique | |
| CN104137773A (en) | Creation method of switchgrass mutants | |
| CN113317158A (en) | Seedling raising method for improving rapid seedling formation of Australian water lily seed seedlings | |
| CN106305147A (en) | Method for using distant complex hybridization technique to cultivate perennial wheat | |
| CN106613689B (en) | Method for rapidly breeding paeonia suffruticosa |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |