CN114317340A - Composite microorganism slow-release column with ceramic membrane as carrier and preparation method thereof - Google Patents
Composite microorganism slow-release column with ceramic membrane as carrier and preparation method thereof Download PDFInfo
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- CN114317340A CN114317340A CN202111596836.9A CN202111596836A CN114317340A CN 114317340 A CN114317340 A CN 114317340A CN 202111596836 A CN202111596836 A CN 202111596836A CN 114317340 A CN114317340 A CN 114317340A
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Abstract
The invention discloses a composite microorganism slow-release column taking a ceramic membrane as a carrier and a preparation method thereof. The composite microorganism slow-release column comprises a ceramic membrane carrier and an internal composite microorganism preparation; the ceramic membrane carrier is a hollow cylinder with holes on two sides, and the composite microbial preparation comprises the following components in parts by weight: 40-60 parts of autotrophic bacteria, 50-150 parts of sulfur powder, 250 parts of pyrite powder and 150-120 parts of zeolite powder. The invention provides a ceramic membrane carrierThe composite microorganism slow-release column can continuously and stably slowly release sulfur autotrophic microorganisms and sulfur-based substances into the added water body, so that NO in water3the-N is subjected to autotrophic denitrification reaction and the phosphate is precipitated, so that the purification rate of pollution indexes such as TN and TP is improved, and the method has the advantages of high reaction rate, good slow release property, strong reproducibility and the like.
Description
Technical Field
The invention relates to a composite microorganism slow-release column, in particular to a composite microorganism slow-release column taking a ceramic membrane as a carrier.
Background
At present, pollution generally exists in main rivers in China, and the point source pollution condition is increasingly prominent. The effluent of the urban sewage treatment plant meets the discharge standard of pollutants for the urban sewage treatment plant, but indexes such as TN and TP specified in the standard are still far lower than V-class water in the quality standard of surface water environment, and the indexes are difficult to be reduced by depending on the self-purification capability of natural water. Therefore, sewage treatment plants need to be improved according to indexes such as TN and TP.
At present, the upgrading and reconstruction of a sewage treatment plant usually adopts a three-stage treatment mode of a coagulation and denitrification filter, the mode needs to continuously add a coagulant and supplement a carbon source to promote the heterotrophic denitrification of microorganisms, the daily operation cost is high, and the whole system has carbon source penetration risk. The sulfur autotrophic denitrification biological treatment mode has the advantages of low sludge yield, low operation cost, no need of adding carbon source and the like, and gradually becomes a hotspot technology for upgrading and modifying sewage plants.
In order to ensure the reaction rate, the specific surface area of a sulfur source donor is considered emphatically by using a sulfur autotrophic denitrification technology, and sulfur source donor substances such as sulfur powder with higher specific surface area and the like are directly added into a water body, so that the phenomenon that the donor is easy to lose along with water is easily caused. Meanwhile, only a sulfur source donor is added into the water body, and the denitrification rate by the natural sulfur autotrophic microorganisms of the water body is low and the utilization rate of the sulfur source is low. The sulfur source donor with the surface coated with the film is added, so that the problems of large occupied area in the film coating process, easy falling of a biological film after being put into use and the like exist, the autotrophic denitrification reaction can only occur on the surface of the sulfur source donor, and the speed is still slow. CN11547839A discloses a composite sulfur-based porous filler, and CN107010724A discloses a slow-release electron donor and a method for deep sewage atmosphere removal by using the same, wherein sulfur is prepared into a similar filler form by adopting a certain means. The former process needs to burn the materials to a molten state, has high operation requirement and risks, and the latter can only purify water flowing through the surface of the sulfur source donor biofilm formation, and the reaction effect has the same limit.
Disclosure of Invention
Aiming at the problems that after a biofilm sulfur source donor is added into a water body, a biomembrane is easy to fall off, a sulfur autotrophic denitrification reaction zone is few and the like in the background technology, the invention provides a composite microorganism slow-release column taking a ceramic membrane as a carrier, which can continuously and stably slowly release sulfur autotrophic microorganisms and sulfur-based substances into the added water body, so that NO in water3the-N is subjected to autotrophic denitrification reaction and the phosphate is precipitated, so that the purification rate of pollution indexes such as TN and TP is improved, and the method has the advantages of high reaction rate, good slow release property, strong reproducibility and the like.
In order to achieve the purpose, the invention adopts the following technical scheme:
a composite microorganism slow-release column with ceramic membrane as carrier comprises ceramic membrane carrier and internal composite microorganism preparation; the ceramic membrane carrier is a hollow cylinder with holes on two sides, and the composite microbial preparation comprises the following components in parts by weight: 40-60 parts of autotrophic bacteria, 50-150 parts of sulfur powder, 250 parts of pyrite powder and 150-120 parts of zeolite powder.
Further, the ceramic membrane has the length of 200-800mm, the outer diameter of the bottom circle of 50-70mm, the inner diameter of 40-60mm, the wall thickness of 10-20mm and the aperture of 40-80 μm.
Furthermore, the fineness of the sulfur powder is 400-600 meshes, the fineness of the pyrite powder is 500-800 meshes, and the fineness of the zeolite powder is 400-600 meshes.
Further, the autotrophic bacteria are one or more of thiobacillus denitrificans, neutral iron oxidizing bacteria and acidophilic iron protoxide thiobacillus.
The invention also provides a preparation method of the composite microorganism sustained-release column taking the ceramic membrane as the carrier, which comprises the following steps:
1) uniformly stirring gypsum powder and 40% of water of the gypsum powder to prepare gypsum slurry, uniformly smearing and plugging the opening on one side of the ceramic membrane carrier, and naturally drying;
2) inoculating autotrophic bacteria into a proper liquid culture medium for activation, and respectively placing the activated bacteria liquid into fermentation tanks for liquid state propagation to obtain autotrophic bacteria liquid;
3) mixing sulfur powder, pyrite powder, zeolite powder and autotrophic bacteria liquid in proportion to obtain a composite microbial preparation;
4) filling the composite microbial preparation into the ceramic membrane carrier from the opening on the other side of the ceramic membrane carrier until the composite microbial preparation is compact, and naturally airing the ceramic membrane carrier after uniformly smearing and plugging the opening by using gypsum slurry.
Further, the number of viable bacteria in the autotrophic bacterial liquid in the step 2) is more than or equal to 5 multiplied by 107cfu/g。
The invention adopts the technical principle that after sulfur autotrophic bacteria and iron autotrophic bacteria are subjected to liquid amplification culture, each bacteria liquid is mixed with sulfur-based donors and substances with certain adsorbability according to different proportions, and the high-efficiency microbial preparation with good pollutant purification effect and high stability is obtained. The microbial preparation is filled into the ceramic membrane carrier with the micro-aperture on the surface, so that the slow release of the microorganisms after being put into the water body can be realized, and the phenomenon that the microorganisms are easy to lose along with water when the microbial preparation is directly added is avoided. After the microorganisms are released, the ceramic membrane carrier can be recovered, and the composite microbial preparation can be filled again after the ceramic membrane carrier is cleaned, so that the average use cost of the ceramic membrane carrier is reduced.
Based on the technical principle, compared with the prior art, the invention has the following beneficial effects:
1) the composite microorganism slow-release column uses NO to treat tail water of sewage treatment plants, rural domestic sewage, low-concentration industrial wastewater and the like3Raw water with main pollutants such as-N, phosphate and the like has good purification capacity, and can effectively reduce pollutant indexes such as TN, TP and the like in the water body.
2) The microbial preparation in the composite microbial slow-release column is simple to manufacture, the loss of internal raw materials cannot be caused in the adding process, and the microbial preparation in the composite microbial slow-release column can be slowly released after being put into water, so that the efficient purification of a target water body is realized.
3) After the composite microorganism slow-release column is put into a target water body or a sewage treatment facility, microorganisms and sulfur-based donors can be slowly released to the surrounding water body, a stable microorganism system can be formed, and long-term and efficient purification of the target water body is facilitated.
Drawings
FIG. 1 is a schematic diagram of a composite microorganism sustained-release column obtained in example 1 of the present invention.
Detailed Description
The technical solutions and effects of the present invention will be further described with reference to the drawings and specific embodiments, but the scope of the present invention is not limited thereto.
The materials used in the following examples, unless otherwise specified, were commercially available from conventional sources.
The detection modes mentioned in the following examples are carried out in accordance with the methods described in the national or industrial standards, unless otherwise specified.
The following examples and illustrations serve to describe and explain the present invention more clearly, but are not to be construed as limiting the invention.
Example 1
A preparation method of a composite microorganism slow-release column taking a ceramic membrane as a carrier comprises the following steps:
1) sealing the one-side opening of the obtained ceramic membrane by gypsum slurry with the water-paste ratio of 40%, and airing for later use, wherein the length of the ceramic membrane is 50cm, the outer diameter of the bottom circle is 60mm, the inner diameter of the bottom circle is 50mm, the wall thickness of the ceramic membrane is 15mm, and the aperture of the ceramic membrane is 60 mu m.
2) And (4) preparing a self-culture liquid culture medium, placing the self-culture liquid culture medium in a 250mL conical flask, and sterilizing.
3) Inoculating purchased thiobacillus denitrificans and neutral iron oxidizing bacteria into a sterilized liquid culture medium, placing the liquid culture medium in a constant-temperature shaking table with the rotation speed of 150rpm and the temperature of 30 ℃, culturing for 72 hours to obtain activated liquid strains, and sealing by adopting an airtight sealing film.
4) According to the formula of the liquid culture medium in the table 1, preparing a growth culture medium in a fermentation tank, respectively inoculating liquid strains after sterilization, and performing fermentation and propagation culture to obtain a thiobacillus denitrificans autotrophic bacterial liquid and a neutral iron oxide bacteria autotrophic bacterial liquid.
5) 100g of purchased 400-mesh sulfur powder, 200g of 600-mesh pyrite powder and 100g of 400-mesh zeolite powder are mixed with 50mL of autotrophic bacteria liquid (in the embodiment, 25mL of each of the two autotrophic bacteria liquid can be mixed in any proportion), the mixture is stirred to be uniform and then is filled into a ceramic membrane column, gypsum slurry is used for sealing the side of the filled side, and the composite microorganism slow-release column is obtained after the mixture is naturally dried, wherein the composite microorganism slow-release column is shown in figure 1.
TABLE 1 autotrophic bacteria liquid Medium composition
Example 2
The aperture of the ceramic membrane adopted by the composite microorganism slow-release column is 80 mu m; the rest is the same as example 1.
Example 3
The composite microorganism slow-release column prepared in the embodiment 1 is used for treating the experiment of simulating the tail water of a sewage treatment plant, and the concrete steps are as follows:
1) standing the composite microorganism slow-release column at the center of a cubic water tank containing 1 cubic meter of water, connecting 1 water pipe with the diameter of 20mm from the bottom of one side of the water tank, and continuously injecting tail water of a simulated sewage treatment plant into the water tank by HRT (Rockwell temperature) 48 h;
2) after 20 days of operation, the bottom of the pool was observed and the water quality index of the inlet and outlet water was measured, the results are shown in Table 2.
Table 2 application example 1 Water quality index of treatment simulation Tail Water experiment
Obvious yellow and black golden muddy substances appear at the bottom of the water tank after 20 days of operation, which shows that the internal sulfur-based donor in the composite microorganism slow-release column is slowly released, and the experimental result shows that the microorganism slow-release column has better effect in the experiment of treating the tail water of the simulated sewage treatment plant, and simulates NO in the tail water3-N is treated more efficiently, NO3The N removal rate reaches 73.5 percent, the TN removal rate reaches 69.0 percent, and the TP removal rate reaches 42.8 percent.
The above-mentioned embodiments only express the specific implementation manner of the present application, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present application; meanwhile, for those skilled in the art, without departing from the technical solution concept of the present application, several changes and modifications are made, which are all within the protection scope of the present application.
Claims (6)
1. A composite microorganism slow-release column taking a ceramic membrane as a carrier is characterized by comprising a ceramic membrane carrier and an internal composite microbial preparation; the ceramic membrane carrier is a hollow cylinder with holes on two sides, and the composite microbial preparation comprises the following components in parts by weight: 40-60 parts of autotrophic bacteria, 50-150 parts of sulfur powder, 250 parts of pyrite powder and 150-120 parts of zeolite powder.
2. The composite microbe controlled-release column using ceramic membrane as carrier as claimed in claim 1, wherein the ceramic membrane has a length of 200-800mm, an outer diameter of 50-70mm, an inner diameter of 40-60mm, a wall thickness of 10-20mm, and an aperture of 40-80 μm.
3. The composite microbial slow-release column with a ceramic membrane as a carrier as claimed in claim 1, wherein the fineness of the sulfur powder is 400-600 meshes, the fineness of the pyrite powder is 500-800 meshes, and the fineness of the zeolite powder is 400-600 meshes.
4. The composite microbial controlled-release column with a ceramic membrane as a carrier according to claim 1, wherein the autotrophic bacteria are one or more of thiobacillus denitrificans, neutrophilic iron-oxidizing bacteria and acidophilic iron-oxidizing thiobacillus.
5. A preparation method of a composite microorganism slow-release column taking a ceramic membrane as a carrier is characterized by comprising the following steps:
1) uniformly stirring gypsum powder and 40% of water of the gypsum powder to prepare gypsum slurry, uniformly smearing and plugging the opening on one side of the ceramic membrane carrier, and naturally drying;
2) inoculating autotrophic bacteria into a proper liquid culture medium for activation, and respectively placing the activated bacteria liquid into fermentation tanks for liquid state propagation to obtain autotrophic bacteria liquid;
3) mixing sulfur powder, pyrite powder, zeolite powder and autotrophic bacteria liquid in proportion to obtain a composite microbial preparation;
4) filling the composite microbial preparation into the ceramic membrane carrier from the opening on the other side of the ceramic membrane carrier until the composite microbial preparation is compact, and naturally airing the ceramic membrane carrier after uniformly smearing and plugging the opening by using gypsum slurry.
6. The method for preparing a composite microbial sustained-release column using a ceramic membrane as a carrier according to claim 5, wherein the step 2)The number of the viable bacteria in the autotrophic bacterial liquid is more than or equal to 5 multiplied by 107 cfu/g。
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117486359A (en) * | 2023-11-16 | 2024-02-02 | 北京沃尔德斯水务科技有限公司 | Sulfur autotrophic denitrification functional biological carrier material and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101973629A (en) * | 2010-10-29 | 2011-02-16 | 南京大学 | Nitrogen and phosphorus removal method by using pyrite as biochemical filling |
| CN206232700U (en) * | 2016-11-30 | 2017-06-09 | 山东山大华特科技股份有限公司 | It is a kind of for sewage disposal with long-acting and slow-release function microorganism generating means |
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- 2021-12-24 CN CN202111596836.9A patent/CN114317340A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101973629A (en) * | 2010-10-29 | 2011-02-16 | 南京大学 | Nitrogen and phosphorus removal method by using pyrite as biochemical filling |
| CN206232700U (en) * | 2016-11-30 | 2017-06-09 | 山东山大华特科技股份有限公司 | It is a kind of for sewage disposal with long-acting and slow-release function microorganism generating means |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117486359A (en) * | 2023-11-16 | 2024-02-02 | 北京沃尔德斯水务科技有限公司 | Sulfur autotrophic denitrification functional biological carrier material and preparation method and application thereof |
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