CN114645041A - Bottom sediment-improved composite microbial inoculum and preparation method thereof - Google Patents
Bottom sediment-improved composite microbial inoculum and preparation method thereof Download PDFInfo
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F11/04—Anaerobic treatment; Production of methane by such processes
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention is suitable for the technical field of bottom sediment remediation, and provides a bottom sediment improvement composite microbial inoculum and a preparation method thereof, wherein the preparation method comprises the following steps: s1, performing bacteria liquid amplification culture; s2, mixed culture; s3, preparing a carrier; s4, loading the microbial inoculum, wherein when the bottom sediment improvement composite microbial inoculum and the product obtained by the preparation method provided by the invention are used, aerobic bacteria increase the content of aerobic microorganisms on the surface layer of the bottom sediment, and anaerobic bacteria are added to increase the content of anaerobic microorganisms on the lower layer of the bottom sediment; a large number of microorganisms can be attached to the gaps of the carrier to form a granular structure with the bottom mud, so that the granular anaerobic environment is improved, the activity of anaerobic bacteria is excited, and the degradation efficiency of pollutants is improved; the carrier forms the agglomerate structure with the bed mud, increases the permeability of bed mud, makes the dissolved oxygen in the water permeate the bed mud, increases the dissolved oxygen volume on bed mud top layer, activates the aerobic microorganism who throws at the rapid speed, activates the aerobic microorganism on bed mud top layer simultaneously, increases the aerobic microorganism content on bed mud top layer rapidly, improves the bed mud environment.
Description
Technical Field
The invention relates to the technical field of bottom sediment remediation, in particular to a bottom sediment improvement composite microbial inoculum and a preparation method thereof.
Background
For the treatment and restoration of the river channel polluted for a long time, particularly the industrial polluted river channel, firstly, the bottom mud of the river channel or the residual bottom mud after dredging needs to be stabilized and improved to eliminate the residual pollutants, so that the water body is polluted again, good conditions are created for the subsequent restoration, and a diversified environment beneficial to the growth of microorganisms is created, therefore, the bottom mud restoration is very important.
The bottom sediment improver is one of important means for bottom sediment remediation, the existing bottom sediment improver is mostly a chemical component, when the bottom sediment improver is used, the remediation process is slow, the conditions are harsh, the remediation effect is poor, and the chemical component in the bottom sediment improver is easy to remain in a water body to cause secondary pollution.
Disclosure of Invention
The invention aims to provide a bottom sediment improvement composite microbial inoculum and a preparation method thereof, and aims to solve the problems that in the prior art, most of bottom sediment improvers are chemical components, when the bottom sediment improvers are used, the restoration process is slow, the conditions are harsh, the restoration effect is poor, and the chemical components in the bottom sediment improvers are easy to remain in a water body to cause secondary pollution.
In order to achieve the purpose, the invention provides the following technical scheme: a bottom sediment improvement composite microbial inoculum and a preparation method thereof comprise the following steps:
s1, bacterial liquid amplification culture:
respectively carrying out amplification culture on yeast, lactobacillus, photosynthetic bacteria, denitrifying bacteria, bacillus and nitrobacteria until the number of effective live bacteria in the bacteria liquid is detected to be more than or equal to 2 multiplied by 108CFU/mL；
S2, mixed culture:
mixing yeast, lactobacillus, photosynthetic bacteria, denitrifying bacteria, bacillus and nitrobacteria liquid in proportion, performing mixed culture, and fermenting to obtain a composite microbial agent;
s3, preparing a carrier:
s31, dehydrating the activated sludge to obtain dehydrated activated sludge;
s32, uniformly mixing the dehydrated activated sludge obtained in the step S31 with high-viscosity attapulgite powder, particle-shaped activated carbon and fly ash in proportion to prepare a modified biological source porous material serving as a carrier;
s4, loading the microbial inoculum:
soaking the modified biological source porous material in a composite microbial inoculum for microbial inoculum loading, wherein the loading condition is carried out under a natural environment condition for 72-96 h, and after the loading is finished, carrying out air drying treatment on the modified biological source porous material.
Preferably, the time for mixed culture in S2 is 3-5 days.
Preferably, the temperature of the mixed culture in S2 is 30 ℃.
Preferably, the moisture content of the dewatered activated sludge in S31 is < 30%.
Preferably, the content of each component in the S32 is as follows in parts by mass:
10-20 parts of high-viscosity attapulgite powder, 30-50 parts of particle-shaped activated carbon, 10-20 parts of fly ash and 5-10 parts of dehydrated activated sludge.
A bottom mud improved composite microbial inoculum comprises anaerobic bacteria and aerobic bacteria;
wherein the anaerobic bacteria include yeast, lactic acid bacteria, photosynthetic bacteria and denitrifying bacteria;
wherein the aerobic bacteria include Bacillus and nitrifying bacteria
Preferably, the content of the anaerobic bacteria and the aerobic bacteria is as follows by mass percent:
70-80% of anaerobic bacteria and 20-30% of aerobic bacteria;
wherein, the anaerobic bacteria comprises the following components in parts by weight: 10-20 parts of saccharomycetes, 10-20 parts of lactic acid bacteria, 20-30 parts of photosynthetic bacteria and 20-30 parts of denitrifying bacteria;
wherein the aerobic bacteria comprise the following components in parts by weight: 10-20 parts of bacillus and 5-10 parts of denitrifying bacteria.
The invention has at least the following beneficial effects:
(1) when the bottom sludge improved composite microbial inoculum and the product obtained by the preparation method provided by the invention are used, aerobic bacteria increase the content of aerobic microorganisms on the surface layer of the bottom sludge, and anaerobic bacteria are added to increase the content of anaerobic microorganisms on the middle and lower layers of the bottom sludge;
(2) when the bottom sediment-improving composite microbial inoculum and the product prepared by the preparation method provided by the invention are used, a large number of microorganisms can be attached to gaps of the carrier to form a granular structure with the bottom sediment, so that the granular anaerobic environment is improved, the activity of anaerobic bacteria is excited, and the degradation efficiency of pollutants is improved;
(3) when the bottom sediment-improving composite microbial inoculum and the product prepared by the preparation method provided by the invention are used, the carrier and the bottom sediment form a granular structure to cover the bottom sediment, so that the pollutants in the bottom sediment are prevented from being rapidly released to a water body;
(4) when the product obtained by the bottom sediment-improving composite microbial agent and the preparation method provided by the invention is used, the carrier and the bottom sediment form a granular structure, so that the permeability of the bottom sediment is increased, dissolved oxygen in a water body is enabled to permeate into the bottom sediment, the dissolved oxygen amount of the surface layer of the bottom sediment is increased, the added aerobic microorganisms are rapidly activated, the indigenous aerobic microorganisms of the surface layer of the bottom sediment are simultaneously activated, the content of the aerobic microorganisms of the surface layer of the bottom sediment is rapidly increased, and the environment of the bottom sediment is improved.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A bottom mud improved composite microbial inoculum comprises anaerobic bacteria and aerobic bacteria;
wherein the anaerobic bacteria include yeast, lactic acid bacteria, photosynthetic bacteria and denitrifying bacteria;
wherein the aerobic bacteria comprise bacillus and nitrobacteria.
Wherein, the contents of anaerobic bacteria and aerobic bacteria are as follows by mass percent:
70% of anaerobic bacteria and 30% of aerobic bacteria;
wherein, the anaerobic bacteria comprises the following components in parts by weight: 10 parts of microzyme, 10 parts of lactic acid bacteria, 20 parts of photosynthetic bacteria and 20 parts of denitrifying bacteria;
wherein the aerobic bacteria comprise the following components in parts by weight: 10 parts of bacillus and 5 parts of denitrifying bacteria.
A method for preparing a bottom sediment improvement composite microbial inoculum,
s1, bacterial liquid amplification culture:
respectively carrying out amplification culture on yeast, lactobacillus, photosynthetic bacteria, denitrifying bacteria, bacillus and nitrobacteria until the number of effective live bacteria in the bacteria liquid is detected to be more than or equal to 2 multiplied by 108CFU/mL；
S2, mixed culture:
mixing yeast, lactobacillus, photosynthetic bacteria, denitrifying bacteria, bacillus and nitrobacteria liquid in proportion, performing mixed culture, and fermenting to obtain a composite microbial inoculum, wherein the mixed culture time is 3-5 days, and the mixed culture temperature is 30 ℃;
s3, preparing a carrier:
s31, dehydrating the activated sludge to enable the water content to be less than 30% to obtain dehydrated activated sludge;
s32, taking 10 parts of high-viscosity attapulgite powder, 30 parts of particle-shaped activated carbon, 10 parts of fly ash and 5 parts of dehydrated activated sludge in parts by mass, and uniformly mixing to prepare a modified biological source porous material serving as a carrier;
s4, loading the microbial inoculum:
soaking the modified biological source porous material in a composite microbial inoculum for microbial inoculum loading, wherein the loading condition is carried out under a natural environment condition for 72-96 h, and after the loading is finished, carrying out air drying treatment on the modified biological source porous material.
Example 2
A bottom mud improved composite microbial inoculum comprises anaerobic bacteria and aerobic bacteria;
wherein the anaerobic bacteria include yeast, lactic acid bacteria, photosynthetic bacteria and denitrifying bacteria;
wherein the aerobic bacteria comprise bacillus and nitrobacteria.
Wherein, the contents of anaerobic bacteria and aerobic bacteria are as follows by mass percent:
70% of anaerobic bacteria and 30% of aerobic bacteria;
wherein, the anaerobic bacteria comprises the following components in parts by weight: 15 parts of yeast, 14 parts of lactic acid bacteria, 23 parts of photosynthetic bacteria and 26 parts of denitrifying bacteria;
wherein the aerobic bacteria comprise the following components in parts by weight: 16 parts of bacillus and 8 parts of denitrifying bacteria.
A method for preparing a bottom sediment improved composite microbial inoculum,
s1, bacterial liquid amplification culture:
respectively carrying out amplification culture on yeast, lactobacillus, photosynthetic bacteria, denitrifying bacteria, bacillus and nitrobacteria until the number of effective live bacteria in the bacteria liquid is detected to be more than or equal to 2 multiplied by 108CFU/mL；
S2, mixed culture:
mixing yeast, lactobacillus, photosynthetic bacteria, denitrifying bacteria, bacillus and nitrobacteria liquid in proportion, performing mixed culture, and fermenting to obtain a composite microbial inoculum, wherein the mixed culture time is 3-5 days, and the mixed culture temperature is 30 ℃;
s3, preparing a carrier:
s31, dehydrating the activated sludge to enable the water content to be less than 30% to obtain dehydrated activated sludge;
s32, taking 12 parts of high-viscosity attapulgite powder, 37 parts of particle-shaped activated carbon, 16 parts of fly ash and 9 parts of dehydrated activated sludge in parts by mass, and uniformly mixing to prepare a modified biological source porous material serving as a carrier;
s4, loading the microbial inoculum:
soaking the modified biological source porous material in a composite microbial inoculum for microbial inoculum loading, wherein the loading condition is carried out under a natural environment condition for 72-96 h, and after the loading is finished, carrying out air drying treatment on the modified biological source porous material.
Example 3
A bottom mud improved composite microbial inoculum comprises anaerobic bacteria and aerobic bacteria;
wherein the anaerobic bacteria include yeast, lactic acid bacteria, photosynthetic bacteria and denitrifying bacteria;
wherein the aerobic bacteria comprise bacillus and nitrobacteria.
Wherein, the contents of anaerobic bacteria and aerobic bacteria are as follows by mass percent:
80% of anaerobic bacteria and 20% of aerobic bacteria;
wherein, the anaerobic bacteria comprises the following components in parts by weight: 13 parts of yeast, 12 parts of lactic acid bacteria, 24 parts of photosynthetic bacteria and 22 parts of denitrifying bacteria;
wherein the aerobic bacteria comprise the following components in parts by weight: 17 parts of bacillus and 6 parts of denitrifying bacteria.
A method for preparing a bottom sediment improved composite microbial inoculum,
s1, bacterial liquid amplification culture:
respectively carrying out amplification culture on yeast, lactobacillus, photosynthetic bacteria, denitrifying bacteria, bacillus and nitrobacteria until the number of effective live bacteria in the bacteria liquid is detected to be more than or equal to 2 multiplied by 108CFU/mL；
S2, mixed culture:
mixing yeast, lactobacillus, photosynthetic bacteria, denitrifying bacteria, bacillus and nitrobacteria liquid in proportion, performing mixed culture, and fermenting to obtain a composite microbial inoculum, wherein the mixed culture time is 3-5 days, and the mixed culture temperature is 30 ℃;
s3, preparing a carrier:
s31, dehydrating the activated sludge to enable the water content to be less than 30% to obtain dehydrated activated sludge;
s32, taking 18 parts of high-viscosity attapulgite powder, 43 parts of particle-shaped activated carbon, 16 parts of fly ash and 9 parts of dehydrated activated sludge in parts by mass, and uniformly mixing to prepare a modified biological source porous material serving as a carrier;
s4, loading the microbial inoculum:
soaking the modified biological source porous material in a composite microbial inoculum for microbial inoculum loading, wherein the loading condition is carried out under a natural environment condition for 72-96 h, and after the loading is finished, carrying out air drying treatment on the modified biological source porous material.
Example 4
A bottom mud improved composite microbial inoculum comprises anaerobic bacteria and aerobic bacteria;
wherein the anaerobic bacteria include yeast, lactic acid bacteria, photosynthetic bacteria and denitrifying bacteria;
wherein the aerobic bacteria comprise bacillus and nitrobacteria.
Wherein, the contents of anaerobic bacteria and aerobic bacteria are as follows by mass percent:
80% of anaerobic bacteria and 20% of aerobic bacteria;
wherein, the anaerobic bacteria comprises the following components in parts by weight: 20 parts of microzyme, 20 parts of lactic acid bacteria, 30 parts of photosynthetic bacteria and 30 parts of denitrifying bacteria;
wherein the aerobic bacteria comprise the following components in parts by weight: 20 parts of bacillus and 10 parts of denitrifying bacteria.
A method for preparing a bottom sediment improved composite microbial inoculum,
s1, bacterial liquid amplification culture:
respectively carrying out amplification culture on yeast, lactobacillus, photosynthetic bacteria, denitrifying bacteria, bacillus and nitrobacteria until the number of effective live bacteria in the bacteria liquid is detected to be more than or equal to 2 multiplied by 108CFU/mL；
S2, mixed culture:
mixing yeast, lactobacillus, photosynthetic bacteria, denitrifying bacteria, bacillus and nitrobacteria liquid in proportion, performing mixed culture, and fermenting to obtain a composite microbial inoculum, wherein the mixed culture time is 3-5 days, and the mixed culture temperature is 30 ℃;
s3, preparing a carrier:
s31, dehydrating the activated sludge to enable the water content to be less than 30% to obtain dehydrated activated sludge;
s32, taking 20 parts of high-viscosity attapulgite powder, 50 parts of particle-shaped activated carbon, 20 parts of fly ash and 10 parts of dehydrated activated sludge in parts by mass, and uniformly mixing to prepare a modified biological source porous material serving as a carrier;
s4, loading the microbial inoculum:
soaking the modified biological source porous material in a composite microbial inoculum for microbial inoculum loading, wherein the loading condition is carried out under a natural environment condition for 72-96 h, and after the loading is finished, carrying out air drying treatment on the modified biological source porous material.
The product prepared by the above embodiments 1-4 has efficient bottom mud repairing capability, is simple to operate, high in repairing speed, good in repairing effect, ecological and environment-friendly, and does not cause secondary pollution.
While there have been shown and described what are at present considered to be the basic principles and essential features of the invention and advantages thereof, it will be apparent to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, but is capable of other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. A preparation method of a bottom sediment-improved composite microbial inoculum is characterized by comprising the following steps:
s1, bacterial liquid amplification culture:
respectively carrying out amplification culture on saccharomycetes, lactic acid bacteria, photosynthetic bacteria, denitrifying bacteria, bacillus and nitrobacteria until the number of effective live bacteria in the bacteria liquid is detected to be more than or equal to 2 multiplied by 108CFU/mL；
S2, mixed culture:
proportionally mixing yeast, lactobacillus, photosynthetic bacteria, denitrifying bacteria, bacillus and nitrobacteria, performing mixed culture, and fermenting to obtain a composite microbial inoculum;
s3, preparing a carrier:
s31, dehydrating the activated sludge to obtain dehydrated activated sludge;
s32, uniformly mixing the dehydrated activated sludge obtained in the step S31 with high-viscosity attapulgite powder, particle-shaped activated carbon and fly ash in proportion to prepare a modified biological source porous material serving as a carrier;
s4, loading the microbial inoculum:
soaking the modified biological source porous material in a composite microbial inoculum for microbial inoculum loading, wherein the loading condition is carried out under a natural environment condition for 72-96 h, and after the loading is finished, carrying out air drying treatment on the modified biological source porous material.
2. The method for preparing a composite bacterial agent for improving bottom sediment according to claim 1, wherein the time for mixed culture in S2 is 3-5 days.
3. The method for preparing a composite bacterial preparation for improving bottom sediment according to claim 1, wherein the temperature of mixed culture in S2 is 30 ℃.
4. The method for preparing a composite bacterial agent for improving bottom sediment according to claim 1, wherein the moisture content of the dewatered activated sludge in the S31 is less than 30%.
5. The preparation method of the bottom sediment-improving composite microbial inoculum according to claim 1, wherein the S32 comprises the following components in parts by mass:
10-20 parts of high-viscosity attapulgite powder, 30-50 parts of particle-shaped activated carbon, 10-20 parts of fly ash and 5-10 parts of dehydrated activated sludge.
6. The bottom sediment improvement composite microbial inoculum according to any one of claims 1 to 5, which comprises anaerobic bacteria and aerobic bacteria;
wherein the anaerobic bacteria include yeast, lactic acid bacteria, photosynthetic bacteria and denitrifying bacteria;
wherein the aerobic bacteria comprise bacillus and nitrobacteria.
7. The bottom sediment improvement composite microbial inoculum according to claim 6, wherein the contents of anaerobic bacteria and aerobic bacteria are as follows by mass percent:
70-80% of anaerobic bacteria and 20-30% of aerobic bacteria;
wherein, the anaerobic bacteria comprises the following components in parts by weight: 10-20 parts of saccharomycetes, 10-20 parts of lactic acid bacteria, 20-30 parts of photosynthetic bacteria and 20-30 parts of denitrifying bacteria;
wherein the aerobic bacteria comprise the following components in parts by weight: 10-20 parts of bacillus and 5-10 parts of denitrifying bacteria.
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Cited By (1)
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| CN115872578A (en) * | 2022-08-30 | 2023-03-31 | 中交第二航务工程局有限公司 | Sediment modifier for improving survival rate of aquatic plants and application thereof |
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