CN114190467A - Preparation method of high-quality rice edible protein powder - Google Patents

Preparation method of high-quality rice edible protein powder Download PDF

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Publication number
CN114190467A
CN114190467A CN202111340520.3A CN202111340520A CN114190467A CN 114190467 A CN114190467 A CN 114190467A CN 202111340520 A CN202111340520 A CN 202111340520A CN 114190467 A CN114190467 A CN 114190467A
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Prior art keywords
protein
rice
protein powder
edible
temperature
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CN202111340520.3A
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陈智桥
肖利平
梅显龙
陈广逵
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Anhui Xingzhou Medicine Food Co ltd
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Anhui Xingzhou Medicine Food Co ltd
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Priority to CN202111340520.3A priority Critical patent/CN114190467A/en
Publication of CN114190467A publication Critical patent/CN114190467A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/12Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from cereals, wheat, bran, or molasses
    • A23J1/125Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from cereals, wheat, bran, or molasses by treatment involving enzymes or microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23JPROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00Working-up of proteins for foodstuffs
    • A23J3/14Vegetable proteins

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Microbiology (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Cereal-Derived Products (AREA)

Abstract

The invention relates to the technical field of protein powder processing, in particular to a preparation method of high-quality rice edible protein powder, which comprises the following steps: s1, soaking and grinding into slurry; s2, size mixing; s3, liquefying and preserving heat; s4, isoelectric deslagging; s5, primary slag washing; s6, performing enzymolysis reaction; s7, enzyme deactivation and sterilization; s8, equal-voltage slag; s9, drying by flash airflow; s10, sieving and coating pulp. According to the preparation method of the high-quality rice edible protein powder, the temperature of production materials is always controlled below 80 ℃, so that protein is not solidified, the functionality of rice protein is well maintained, the high quality of the rice protein is ensured, rice embryo protein can be produced by further adding rice protease for hydrolysis, and the conversion rate is improved; the process of the invention can lead the protein content to reach more than 80 percent under the condition of medium temperature and the combined action of a plurality of enzymes, can meet the requirements of edible protein and raw materials for producing rice embryo protein, and can produce the protein content which can not be produced by a centrifugal separation method.

Description

Preparation method of high-quality rice edible protein powder
Technical Field
The invention relates to the technical field of protein powder processing, in particular to a preparation method of high-quality rice edible protein powder.
Background
In recent years, with the continuous improvement of living standard of people, the demand of protein for one of three nutrients is more and more, and the quality requirement is more and more high. The main protein sources ingested by people, namely animal proteins (meat, eggs and milk), are influenced by animal infectious diseases (mad cow disease, foot and mouth disease, avian influenza and swine fever), so that the supply of the animal proteins is unstable and the safety risk exists. Thus, the demand for protein is shifted from a large amount of animal protein to vegetable protein.
The rice protein powder contains amino acids necessary for human bodies, is complete protein, has low fat, no alcohol and low allergy, has the highest amino acid content in cereal protein, has reasonable amino acid composition proportion, accords with the nutrition mode recommended by FAO/WHO (world food organization/world health organization), has the TD value (protein digestibility) of 82, the BV value (protein biological value) of 77, the PER value (protein power ratio) of 1.7 and the nutritional value far higher than that of other plant eggs, can be compared favorably with high-quality proteins such as eggs, milk, beef, fish, shrimp and the like, and has extremely good development prospect in the food market.
The present production method of edible rice protein powder mainly includes the steps of grinding rice milk, adopting centrifugal separation method of disk centrifuge and adopting high-temperature resistant amylase to liquefy rice milk. The centrifugal separation method has the defects that the separation effect of the rice protein and the rice starch is poor, the content of the rice protein is only 50 percent, the content requirement of the edible rice protein (the content is more than or equal to 80 percent) cannot be met, the protein recovery rate is low, and the large-scale industrial production is not realized. The high-temperature enzyme method has the defects that the rice protein is liquefied at high temperature (below 105 ℃) and is preserved, so that the rice protein is solidified at high temperature, the original functionality (water retention property, foaming property, emulsifying property, cohesiveness, gel formation, fiber film formation and other properties) of the rice protein is damaged, and the rice germ protein is further produced, so that the proteolysis reaction and the solubility are very difficult.
Aiming at the defects of the prior art, the preparation method of the high-quality rice edible protein powder is provided under the medium temperature condition.
Disclosure of Invention
The invention provides a preparation method of high-quality rice edible protein powder aiming at the defects of the prior art.
The invention solves the technical problems through the following technical means:
a preparation method of high-quality rice edible protein powder comprises the following steps:
s1, soaking and grinding: washing rice with water, soaking in water for 2-4 hr, and grinding to fineness of over 80 mesh;
s2, size mixing: adjusting the concentration of the milled rice milk to 17-18Be DEG, adjusting the pH value to 5.0-6.0, adding 6-8 enzyme units of medium temperature amylase per gram of dry matter, and stirring uniformly;
s3, liquefying and preserving heat: injecting the rice milk after size mixing into a liquefaction injector for liquefaction, outputting the rice milk into a laminar flow heat preservation tank by the liquefaction injector after the temperature is 70-80 ℃, preserving the heat for 2-3 hours, and completing liquefaction and heat preservation after the iodine test reaction is brown yellow or yellow;
s4, isoelectric deslagging: the liquefied material enters a transfer tank, the PH value is adjusted to 4.7-4.8, and a plate-frame filter is used for filtering and removing slag;
s5, primary slag washing: conveying the isoelectric deslagged rice protein into a primary residue washing tank, adding water, stirring, heating to 70 deg.C, and filtering with a plate-and-frame filter to obtain rice protein extract with concentration of 20-25%;
s6, enzymolysis reaction: conveying the rice protein subjected to primary slag washing to an enzymolysis tank, adding hot water, stirring until the concentration is 20-25%, heating to 70 ℃, adjusting the pH value to 5.0-6.0, adding medium-temperature amylase 3-4 enzyme units/gram dry matter, adding cellulase 3-4 enzyme units/gram dry matter, and carrying out heat preservation reaction for 2 hours;
s7, enzyme inactivation and sterilization: heating the rice protein after the enzymolysis reaction to 80 ℃ in an enzymolysis tank, and maintaining for 30 minutes;
s8, iso-voltage slag: adjusting pH of the sterilized rice protein in an enzymolysis tank to 4.7-4.8, filtering with a plate-and-frame filter, pressing the plate-and-frame filter, and flushing water in the plate-and-frame protein residue with compressed air;
s9, drying by flash airflow: conveying the rice protein from the protein residue after the residue is subjected to the equal voltage to a drying device by an auger for airflow drying until the drying moisture of the protein powder is 4-6%;
s10, sieving and pulp wrapping: and sieving the protein powder crushed by the flash evaporation high-speed blade by using a 100-mesh vibrating screen, and coating the sieved protein powder by using a vacuum slurry coating machine.
As an improvement of the above technical solution, in step S1, the refining method is any one of a pin mill and a colloid mill.
As an improvement of the above technical solution, in the step S2, any one of edible soda ash and edible citric acid is used for adjusting the PH of the rice milk.
As an improvement of the above technical solution, in the step S9, the airflow drying is performed in two steps, the temperature of the primary hot air is 100-120 ℃, and the temperature of the secondary hot air is 80-120 ℃.
The invention has the beneficial effects that:
1. the temperature of the production materials is controlled below 80 ℃ all the time, so that protein is not solidified, the functionality of the rice protein is well maintained, the high quality of the rice protein is ensured, the rice germ protein can be produced by further adding rice protease for hydrolysis, and the conversion rate is improved;
2. the process reaction temperature is 80 ℃, and compared with the arrangement that the process reaction temperature is 105 ℃ in a high-temperature enzyme method, the process can greatly save energy (heat energy is saved by heating, water is saved by cooling);
3. the process of the invention can lead the protein content to reach more than 80 percent under the condition of medium temperature and the combined action of a plurality of enzymes, can meet the requirements of edible protein and raw materials for producing rice embryo protein, and can produce the protein content which can not be produced by a centrifugal separation method;
4. isoelectric filtration is adopted, the isoelectric point of the protein is adjusted, and filtration is carried out when the solubility of the protein is the lowest, so that the recovery rate of the protein can be improved;
5. the process of the invention does not need high temperature, and does not add strong acid and strong alkali, and the produced finished product protein powder has good taste and pure appearance color;
6. the special flash airflow drying is adopted, the protein temperature can not exceed 80 ℃ when the protein powder is dried, two air exhaust channels are used, and the flash high-speed blades are used for crushing, so that the fineness of the finished product protein powder is high, the appearance color is attractive, and the high quality of the protein powder is ensured at low temperature.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are a part of the embodiments of the present invention, but not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present.
Examples
The preparation method of the high-quality rice edible protein powder comprises the following steps:
s1, soaking and grinding: washing rice with water, soaking in water for 2-4 hr, and grinding to fineness of over 80 mesh;
s2, size mixing: adjusting the concentration of the milled rice milk to 17-18Be DEG, adjusting the pH value to 5.0-6.0, adding 6-8 enzyme units of medium temperature amylase per gram of dry matter, and stirring uniformly;
s3, liquefying and preserving heat: injecting the rice milk after size mixing into a liquefaction injector for liquefaction, outputting the rice milk into a laminar flow heat preservation tank by the liquefaction injector after the temperature is 70-80 ℃, preserving the heat for 2-3 hours, and completing liquefaction and heat preservation after the iodine test reaction is brown yellow or yellow;
s4, isoelectric deslagging: the liquefied material enters a transfer tank to adjust the pH value to 4.7-4.8 (reaching the isoelectric pH of rice protein of 4.75), and a plate-frame filter is used for filtering and deslagging;
s5, primary slag washing: conveying the isoelectric deslagged rice protein into a primary residue washing tank, adding water, stirring, heating to 70 deg.C, and filtering with a plate-and-frame filter to obtain rice protein extract with concentration of 20-25%;
s6, enzymolysis reaction: conveying the rice protein subjected to primary slag washing to an enzymolysis tank, adding hot water, stirring until the concentration is 20-25%, heating to 70 ℃, adjusting the pH value to 5.0-6.0, adding medium-temperature amylase 3-4 enzyme units/gram dry matter, adding cellulase 3-4 enzyme units/gram dry matter, and carrying out heat preservation reaction for 2 hours;
s7, enzyme inactivation and sterilization: heating the rice protein after the enzymolysis reaction to 80 ℃ in an enzymolysis tank, and maintaining for 30 minutes;
s8, iso-voltage slag: adjusting pH of the sterilized rice protein in an enzymolysis tank to 4.7-4.8, filtering with a plate-and-frame filter, pressing the plate-and-frame filter, and flushing water in the plate-and-frame protein residue with compressed air;
s9, drying by flash airflow: conveying the rice protein (the water content is less than or equal to 45 percent and the protein content is more than or equal to 81 percent) of the protein residue after the residue with the equal voltage into a drying device for air flow drying until the drying water content of the protein powder is 4-6 percent;
s10, sieving and pulp wrapping: and sieving the protein powder crushed by the flash evaporation high-speed blade by using a 100-mesh vibrating screen, and coating the sieved protein powder by using a vacuum slurry coating machine.
In step S1, the refining method is performed by any one of a pin mill and a colloid mill.
In step S2, either edible soda ash or edible citric acid is used to adjust the PH of the rice milk.
In step S9, the airflow drying is performed in two steps, the temperature of the primary hot air is 100-120 ℃, and the temperature of the secondary hot air is 80-120 ℃.
The preparation method of the high-quality rice edible protein powder has the following advantages,
1. the temperature of the production materials is controlled below 80 ℃ all the time, so that protein is not solidified, the functionality of the rice protein is well maintained, the high quality of the rice protein is ensured, the rice germ protein can be produced by further adding rice protease for hydrolysis, and the conversion rate is improved;
3. the process reaction temperature is 80 ℃, and compared with the arrangement that the process reaction temperature is 105 ℃ in a high-temperature enzyme method, the process can greatly save energy (heat energy is saved by heating, water is saved by cooling);
3. the process of the invention can lead the protein content to reach more than 80 percent under the condition of medium temperature and the combined action of a plurality of enzymes, can meet the requirements of edible protein and raw materials for producing rice embryo protein, and can produce the protein content which can not be produced by a centrifugal separation method;
4. isoelectric filtration is adopted, the isoelectric point of the protein is adjusted, and filtration is carried out when the solubility of the protein is the lowest, so that the recovery rate of the protein can be improved;
5. the process of the invention does not need high temperature, and does not add strong acid and strong alkali, and the produced finished product protein powder has good taste and pure appearance color;
6. the special flash airflow drying is adopted, the protein temperature can not exceed 80 ℃ when the protein powder is dried, two air exhaust channels are used, and the flash high-speed blades are used for crushing, so that the fineness of the finished product protein powder is high, the appearance color is attractive, and the high quality of the protein powder is ensured at low temperature.
Experimental cases of the present invention:
weighing 1000g of rice, soaking for 2 hours, grinding with boiled water by using an experimental colloid mill, and sieving with a 80-mesh sieve (grinding coarse grains again and sieving once); preparing into 3000ml, Baume concentration 17.5Be, adjusting pH value to 5.4, heating to 75 deg.C in water bath, adding 3.5ml of medium temperature amylase, stirring for 2 hr, and reacting with iodine to obtain yellow; adjusting pH to 4.75 with 10% citric acid, vacuum filtering with filter funnel, and centrifuging protein residue to water content of less than 50%; preparing 500ml of the mixture by using 80 ℃ hot water, stirring the mixture for 20 minutes at the temperature of 75 ℃, adding filter paper into a filter funnel, and performing suction filtration by using a vacuum pump; sampling to determine the protein content to be 68.5%; preparing the mixture into a volume of 500ml by using hot water at the temperature of 80 ℃, adjusting the pH value to 5.5, keeping the temperature in a water bath kettle at 70 ℃, adding 2ml of medium-temperature amylase and 3ml of cellulase, performing heat preservation reaction for 2 hours, adjusting the pH value to 4.75, heating to 80 ℃, preserving the temperature for 30 minutes, performing vacuum filtration, spin-drying by using a centrifuge until the water content is less than or equal to 50 percent, drying in an oven at the temperature of 75 ℃, and crushing by using a universal crusher; the water content is measured to be 5.2 percent, the protein content is 83 percent, the microorganism control is qualified, and the weight is 85 g.
It is noted that, in this document, relational terms such as first and second, and the like, if any, are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.

Claims (4)

1. A preparation method of high-quality rice edible protein powder is characterized by comprising the following steps: the method comprises the following steps:
s1, soaking and grinding: washing rice with water, soaking in water for 2-4 hr, and grinding to fineness of over 80 mesh;
s2, size mixing: adjusting the concentration of the milled rice milk to 17-18Be DEG, adjusting the pH value to 5.0-6.0, adding 6-8 enzyme units of medium temperature amylase per gram of dry matter, and stirring uniformly;
s3, liquefying and preserving heat: injecting the rice milk after size mixing into a liquefaction injector for liquefaction, outputting the rice milk into a laminar flow heat preservation tank by the liquefaction injector after the temperature is 70-80 ℃, preserving the heat for 2-3 hours, and completing liquefaction and heat preservation after the iodine test reaction is brown yellow or yellow;
s4, isoelectric deslagging: the liquefied material enters a transfer tank, the PH value is adjusted to 4.7-4.8, and a plate-frame filter is used for filtering and removing slag;
s5, primary slag washing: conveying the isoelectric deslagged rice protein into a primary residue washing tank, adding water, stirring, heating to 70 deg.C, and filtering with a plate-and-frame filter to obtain rice protein extract with concentration of 20-25%;
s6, enzymolysis reaction: conveying the rice protein subjected to primary slag washing to an enzymolysis tank, adding hot water, stirring until the concentration is 20-25%, heating to 70 ℃, adjusting the pH value to 5.0-6.0, adding medium-temperature amylase 3-4 enzyme units/gram dry matter, adding cellulase 3-4 enzyme units/gram dry matter, and carrying out heat preservation reaction for 2 hours;
s7, enzyme inactivation and sterilization: heating the rice protein after the enzymolysis reaction to 80 ℃ in an enzymolysis tank, and maintaining for 30 minutes;
s8, iso-voltage slag: adjusting pH of the sterilized rice protein in an enzymolysis tank to 4.7-4.8, filtering with a plate-and-frame filter, pressing the plate-and-frame filter, and flushing water in the plate-and-frame protein residue with compressed air;
s9, drying by flash airflow: conveying the rice protein from the protein residue after the residue is subjected to the equal voltage to a drying device by an auger for airflow drying until the drying moisture of the protein powder is 4-6%;
s10, sieving and pulp wrapping: and sieving the protein powder crushed by the flash evaporation high-speed blade by using a 100-mesh vibrating screen, and coating the sieved protein powder by using a vacuum slurry coating machine.
2. The preparation method of the high-quality rice edible protein powder as claimed in claim 1, which is characterized in that: in the step S1, the refining method is any one of a pin mill and a colloid mill.
3. The preparation method of the high-quality rice edible protein powder as claimed in claim 1, which is characterized in that: in the step S2, any one of edible soda ash and edible citric acid is used for adjusting the PH of the rice milk.
4. The preparation method of the high-quality rice edible protein powder as claimed in claim 1, which is characterized in that: in the step S9, the airflow drying is performed in two steps, the temperature of the primary hot air is 100-120 ℃, and the temperature of the secondary hot air is 80-120 ℃.
CN202111340520.3A 2021-11-12 2021-11-12 Preparation method of high-quality rice edible protein powder Pending CN114190467A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101695334A (en) * 2009-09-09 2010-04-21 万福生科(湖南)农业开发股份有限公司 Method for extracting rice protein by using rice
CN103330047A (en) * 2013-07-12 2013-10-02 普洱永吉生物技术有限责任公司 Preparation method of rice protein
CN110438189A (en) * 2019-09-11 2019-11-12 安徽焕发生物科技有限公司 A kind of rice protein processing technology
CN111418702A (en) * 2020-04-27 2020-07-17 安徽顺鑫盛源生物食品有限公司 Production process of low-denaturation instant rice protein powder

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101695334A (en) * 2009-09-09 2010-04-21 万福生科(湖南)农业开发股份有限公司 Method for extracting rice protein by using rice
CN103330047A (en) * 2013-07-12 2013-10-02 普洱永吉生物技术有限责任公司 Preparation method of rice protein
CN110438189A (en) * 2019-09-11 2019-11-12 安徽焕发生物科技有限公司 A kind of rice protein processing technology
CN111418702A (en) * 2020-04-27 2020-07-17 安徽顺鑫盛源生物食品有限公司 Production process of low-denaturation instant rice protein powder

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