CN108771244A - The preparation method of the oligomeric peptide extract of abalone, method, abalone oligopeptide and its application for preparing abalone oligopeptide and abalone powder - Google Patents
The preparation method of the oligomeric peptide extract of abalone, method, abalone oligopeptide and its application for preparing abalone oligopeptide and abalone powder Download PDFInfo
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- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 88
- 239000000284 extract Substances 0.000 title claims abstract description 81
- 238000000034 method Methods 0.000 title claims abstract description 48
- 239000000843 powder Substances 0.000 title claims abstract description 34
- 238000002360 preparation method Methods 0.000 title claims abstract description 32
- 102000015636 Oligopeptides Human genes 0.000 title claims description 54
- 108010038807 Oligopeptides Proteins 0.000 title claims description 54
- 235000013372 meat Nutrition 0.000 claims abstract description 102
- 239000007788 liquid Substances 0.000 claims abstract description 80
- 108091005804 Peptidases Proteins 0.000 claims abstract description 42
- 239000004365 Protease Substances 0.000 claims abstract description 42
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 42
- 238000004519 manufacturing process Methods 0.000 claims abstract description 38
- 238000002156 mixing Methods 0.000 claims abstract description 38
- 150000001875 compounds Chemical class 0.000 claims abstract description 37
- 239000003337 fertilizer Substances 0.000 claims abstract description 37
- 239000002893 slag Substances 0.000 claims abstract description 26
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 23
- 239000000706 filtrate Substances 0.000 claims abstract description 20
- 239000003513 alkali Substances 0.000 claims abstract description 15
- 238000005406 washing Methods 0.000 claims abstract description 9
- 238000003801 milling Methods 0.000 claims abstract description 8
- 238000001914 filtration Methods 0.000 claims abstract description 7
- 238000001035 drying Methods 0.000 claims abstract description 3
- 238000000498 ball milling Methods 0.000 claims description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 238000006243 chemical reaction Methods 0.000 claims description 13
- 230000008569 process Effects 0.000 claims description 10
- 239000000126 substance Substances 0.000 claims description 10
- 238000012545 processing Methods 0.000 claims description 6
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 4
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 4
- 235000019640 taste Nutrition 0.000 abstract description 10
- 239000000047 product Substances 0.000 abstract description 7
- 239000012528 membrane Substances 0.000 abstract description 6
- 230000036541 health Effects 0.000 abstract description 2
- 230000000717 retained effect Effects 0.000 abstract description 2
- 102000004190 Enzymes Human genes 0.000 description 12
- 108090000790 Enzymes Proteins 0.000 description 12
- 230000007062 hydrolysis Effects 0.000 description 12
- 238000006460 hydrolysis reaction Methods 0.000 description 12
- 102000004196 processed proteins & peptides Human genes 0.000 description 12
- 102000004169 proteins and genes Human genes 0.000 description 11
- 108090000623 proteins and genes Proteins 0.000 description 11
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- 241000251468 Actinopterygii Species 0.000 description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 6
- 239000000796 flavoring agent Substances 0.000 description 6
- 235000019634 flavors Nutrition 0.000 description 6
- 238000003756 stirring Methods 0.000 description 6
- 150000001413 amino acids Chemical class 0.000 description 5
- 238000013461 design Methods 0.000 description 5
- 230000003301 hydrolyzing effect Effects 0.000 description 5
- 230000000050 nutritive effect Effects 0.000 description 5
- 230000002209 hydrophobic effect Effects 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 235000019658 bitter taste Nutrition 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 239000012467 final product Substances 0.000 description 3
- 229920001184 polypeptide Polymers 0.000 description 3
- 235000000346 sugar Nutrition 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 238000004064 recycling Methods 0.000 description 2
- 238000007873 sieving Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 240000006439 Aspergillus oryzae Species 0.000 description 1
- 235000002247 Aspergillus oryzae Nutrition 0.000 description 1
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000009837 dry grinding Methods 0.000 description 1
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- 150000002148 esters Chemical class 0.000 description 1
- 239000000413 hydrolysate Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000001471 micro-filtration Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000007065 protein hydrolysis Effects 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 238000009288 screen filtration Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/04—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from fish or other sea animals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/04—Animal proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/65—Addition of, or treatment with, microorganisms or enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
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Abstract
The present invention relates to field of health care products, provide a kind of preparation method of the oligomeric peptide extract of abalone, and preparation process includes：Abalone meat gruel liquid is mixed with the mixing protease that alkali protease and trypsase form, obtains the first mixed enzymolysis liquid；Compound fertilizer production is added into the first mixed enzymolysis liquid, obtains the second mixed enzymolysis liquid；Second mixed enzymolysis liquid is filtered into removal meat slag and obtains filtrate；The oligomeric peptide extract of abalone is retained using ultrafiltration membrane.The present invention also provides a kind of method preparing abalone oligomeric peptide extract and abalone powder, including the preparation method of the oligomeric peptide extract of above-mentioned abalone and by the meat slag being obtained by filtration after washing at least twice, drying milling obtains abalone powder.The oligomeric peptide extract of abalone made from this method have better nutritivity be worth and it is in good taste.The present invention also provides the application of the oligomeric peptide extract of abalone and the extract in tablet and electuary is made made from a kind of preparation method by the oligomeric peptide extract of above-mentioned abalone.
Description
Technical field
The present invention relates to field of health care products, preparation method, preparation abalone in particular to the oligomeric peptide extract of abalone
The method of oligopeptide and abalone powder, abalone oligopeptide and its application.
Background technology
Abalone oligopeptide is easily absorbed by the body, and has better nutritivity value.In the prior art, the extraction of abalone oligopeptide
It is general that method would generally extract oligomeric peptide extract mouthfeel that is insufficient or obtaining.
In consideration of it, special propose the application.
Invention content
The present invention provides a kind of preparation methods of the oligomeric peptide extract of abalone, it is intended to improve existing extracting method to Bao
Oligopeptide extracts the general problem of oligopeptide mouthfeel that is insufficient, obtaining in fish.
The present invention also provides a kind of method preparing the oligomeric peptide extract of abalone and abalone powder, this method can be extracted fully
Oligopeptide in abalone, and make oligopeptide obtained in good taste, while the abalone powder being worth with better nutritivity can also be made.
It is in good taste the present invention provides a kind of oligomeric peptide extract, it is full of nutrition.
The present invention also provides application of the oligomeric peptide extract in preparing abalone oligopeptide piece, it is applied to prepare abalone
When in oligopeptide piece so that abalone oligopeptide piece obtained is worth with better nutritivity, and in good taste.
The present invention also provides application of the oligomeric peptide extract in preparing abalone oligopeptide electuary, it is applied to prepare Bao
When in fish oligopeptide electuary so that abalone oligopeptide impact obtained has better nutritivity value, and in good taste.
The invention is realized in this way：
A kind of preparation method of the oligomeric peptide extract of abalone, preparation process include：
Liquid processed：Abalone meat gruel and water are mixed to prepare abalone meat gruel liquid, the mass ratio of abalone meat gruel and water is 0.7~1:1；
Primary enzymolysis：By abalone meat gruel liquid and mixing protease with mass ratio 1:0.6~1 mixing, it is 7~8 to adjust pH of mixed, reaction
105~115min obtains the first mixed enzymolysis liquid, and mixing protease includes that mass ratio is 1:0.8~1.2 alkali protease and pancreas
The vigor of protease, alkali protease and trypsase is 2500~2600U/g；Secondary enzymolysis：To the first mixed enzymolysis liquid
Middle addition compound fertilizer production, the addition of compound fertilizer production are 0.8~1 with the mass ratio of abalone meat gruel liquid:1, it adjusts
It is 5.5~6.5 to save pH, and 60~70min of reaction obtains the second mixed enzymolysis liquid, the vigor of compound fertilizer production is 900~
1000U/g；Filtering：Second mixed enzymolysis liquid is filtered into removal meat slag and obtains filtrate；Ultrafiltration：Use ultrafiltration retaining molecular weight for 80
The substance of~1000 dalton obtains the oligomeric peptide extract of abalone.
A method of the oligomeric peptide extract of abalone and abalone powder are prepared, the system of the oligomeric peptide extract of above-mentioned abalone is included
Preparation Method and meat slag procedure of processing, meat slag of the meat slag procedure of processing for that will be obtained by filtration dry milling after washing at least twice
Obtain abalone powder.
The preparation method of a kind of oligomeric peptide extract of abalone, the oligomeric peptide extract of above-mentioned abalone is made.
Application of the oligomeric peptide extract of above-mentioned abalone in preparing abalone oligopeptide piece.
Application of the oligomeric peptide extract of above-mentioned abalone in preparing abalone oligopeptide electuary.
The beneficial effects of the invention are as follows：The preparation side that the present invention passes through the oligomeric peptide extract of abalone that above-mentioned design obtains
Method, due to using once being hydrolyzed by properly mixed alkali protease and trypsase, using compound fertilizer production afterwards
Secondary hydrolysis is carried out, enables to the protein in abalone meat to be fully hydrolyzed for oligopeptide, and hydrolytic process is produced due to one time
Raw a small amount of bitter peptides hydrophobic amino acid in secondary hydrolysis is removed and makes in final product almost without bitter peptides, in turn
So that obtained oligomeric peptide extract is in good taste, and finally obtained certain oligopeptides or compound fertilizer production can be with
Maillard reaction, the mouthfeel of the oligomeric peptide extract of abalone advanced optimized occur for the reduced sugar in abalone meat.
The method for preparing the oligomeric peptide extract of abalone and abalone powder that the present invention is obtained by above-mentioned design, can not only
The oligomeric peptide extract of abalone provided by the invention is made, moreover it is possible to the same abalone powder with nutritive value be made.
The present invention is by the oligomeric peptide extract of abalone that above-mentioned design obtains, since it is almost all oligopeptide,
It is easily absorbed by the body, there is higher nutritive value, while its is in good taste.
When the abalone oligopeptide that the present invention is obtained by above-mentioned design is used to prepare abalone oligopeptide piece so that Bao obtained
Nutriment in fish oligopeptide piece is easy to be absorbed.
When the abalone oligopeptide that the present invention is obtained by above-mentioned design is used to prepare abalone oligopeptide electuary so that obtained
Nutriment in abalone oligopeptide electuary is easy to be absorbed.
Specific implementation mode
It in order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below will be in the embodiment of the present invention
Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, builds according to normal condition or manufacturer
The condition of view carries out.Reagents or instruments used without specified manufacturer is the conventional production that can be obtained by commercially available purchase
Product.
A kind of preparation method of the oligomeric peptide extract of abalone is provided to the embodiment of the present invention below, abalone oligopeptide is prepared and carries
Method, abalone oligopeptide and its application of object and abalone powder is taken to be specifically described.
A kind of preparation method of the oligomeric peptide extract of abalone, preparation process include：
S1, abalone meat gruel and water are mixed to prepare abalone meat gruel liquid, and the mass ratio of abalone meat gruel and water is 0.7~1:1.
First, after the refrigeration abalone after choosing fresh abalone or thawing cleans up, abalone chopping is put into pulverizer
It smashes, then crosses 40 mesh sieve, take and sieve lower meat gruel, meat gruel, which is back in pulverizer, on sieve continues to smash.Abalone meat gruel after sieving
Grain size is small, and hydrolysis is come into full contact with convenient for follow-up enzyme and protein therein.Abalone meat gruel after sieving and water with mass ratio 0.7~
1:1 is uniformly mixed, and when mixing, manually or mechanically stirring 10min or so can be used and obtain abalone meat gruel liquid.
In order to enable abalone meat gruel and water more to mix well, it is preferable that abalone meat gruel liquid can be placed in ball mill
Mixing.It should be noted that can also use homogenizer that abalone meat gruel is made to can be uniformly dispersed in water in the present invention.More there is choosing
Ground, to keep abalone meat gruel and water more fully mixed, abalone meat gruel more full and uniform dispersion in water, temperature when ball milling is-
12~-8 degrees Celsius, 50~80HZ of frequency, 20~120min of Ball-milling Time.
S2, primary enzymolysis：By abalone meat gruel liquid and mixing protease with mass ratio 1:0.6~1 mixing, adjusts pH of mixed
It is 7~8,105~115min of reaction obtains the first mixed enzymolysis liquid, and mixing protease includes that mass ratio is 1:0.8~1.2 alkalinity
The vigor of protease and trypsase, alkali protease and trypsase is 2500~2600U/g.
Mixing protease is configured, is 1 by mass ratio:0.8~1.2, vigor is the alkali protease of 2500~2600U/g
It is uniformly mixed with trypsase, obtains mixing protease.By abalone meat gruel liquid made from S1 and mixing protease with mass ratio 1:
0.6~1 mixing, mixed process make the two be uniformly mixed using artificial or mechanical agitation.Bao is adjusted after mixing protease is added
Sodium hydroxide and hydrochloric acid can be used in the adjusting of the pH to 6~7, pH of fish meat emulsion liquid.PH makes mixing protease and abalone meat after adjusting
Rotten liquid reacts 105~115min to ensure that mixing protease can fully make protein hydrolyze to obtain the first mixed enzymolysis liquid.It is excellent
Selection of land, to ensure that mixed protein enzymatic activity and protein hydrolysis efficiency are higher, during primary enzymolysis, controlled enzymatic hydrolysis temperature is
40~50 DEG C.
S3, secondary enzymolysis：Compound fertilizer production, the addition of compound fertilizer production are added into the first mixed enzymolysis liquid
The mass ratio of amount and abalone meat gruel liquid is 0.8~1:1, it is 5.5~6.5 to adjust pH, reacts 60~70min, obtains the second mixing
The vigor of enzymolysis liquid, compound fertilizer production is 900~1000U/g.
Compound fertilizer production is fermented using aspergillus oryzae, through advanced extraction process micro-filtration, ultrafiltration concentration, dry technology
It refines and obtains, then add some flavor substances, screening is re-dubbed compound fertilizer production.This compound fertilizer production is naturally pacified
Entirely, the hydrolysis of various animal/vegetable proteins is can be applied to, the optimization of later stage flavor removes bitter taste, improves mouthfeel, it is good can to produce flavor
Good animals and plants hydrolysis prods improve product quality, reduce cost.
Compound fertilizer production and then secondary enzymolysis are added in the first mixed enzymolysis liquid obtained to S2, secondary enzymolysis is with one
The first mixed enzymolysis liquid of product of secondary enzymolysis is substrate, is further hydrolyzed again to the protein or polypeptide that are digested.For
Keep secondary enzymolysis more efficient, enzymolysis more fully carries out, the addition of compound fertilizer production and abalone meat gruel liquid in secondary enzymolysis
Mass ratio be 0.8~1:1, it is 5.5~6.5 that secondary enzymolysis adjusts pH in the process, and the enzyme digestion reaction time is that 60~70min is obtained
The vigor of second mixed enzymolysis liquid, the compound fertilizer production is 900~1000U/g.Preferably, it is guarantee composite flavor albumen
The hydrolysis efficiency of enzymatic activity and protein and polypeptide is higher, and during secondary enzymolysis, controlled enzymatic hydrolysis temperature is 45~55 DEG C.
S4, filtering：Second mixed enzymolysis liquid is filtered into removal meat slag and obtains filtrate.
Specifically, using screen filtration secondary enzymolysis products more than 60 mesh, filtrate is obtained, filtrate at this time includes enzymolysis
Various enzymes, water or the hydrochloric acid or sodium hydroxide that adjust the addition of pH processes used in the process.
S5, ultrafiltration：Ultrafiltration retaining molecular weight is used to obtain the extraction of abalone oligopeptide for the substance of 80~1000 dalton
Object.
The filtrate that S4 is obtained is handled using ultrafiltration membrane, molecular cut off is the polypeptide and warp of 80~1000 dalton
The flavor substance that Maillard reaction generates.Specifically, ultra-filtration process at twice, use first molecular cut off for 80 or more it is super
Filter membrane carries out a hyperfiltration treatment to filtrate, and filtrate is water, hydrochloric acid or sodium hydroxide under film, is that membrane part then recycles on film
Second ultrafiltration is carried out, second ultrafiltration uses molecular cut off for 1000 or more ultrafiltration membrane, and filtrate then provides for the present invention under film
The oligomeric peptide extract of abalone, it is the various enzymes used in hydrolytic process not cross membrane part then on film.It can be each by what is retained on film
Recycling is detached after kind enzyme recycling.
Alkali protease has the characteristics that enzyme activity is big, hydrolysis degree is big, but is easy to generate by the protein of its enzymolysis
Bitter peptides, and the characteristic that trypsase has trichloroacetic acid dissolution index high.Therefore the two is carried out mixed enzymolysis by the first stage, it can
Reduce the generation of bitter peptides.And compound fertilizer production is to mix enzyme, acts on the peptide bond of peptide molecule amino or carboxyl terminal, trip
Amino acid is separated out, protease hydrolysate is substantially without bitter taste.The product that composite flavor enzyme is generated using hydrolysis by novo is substrate, bitter taste
It is removed and substantially eliminates with the hydrophobic amino acid of bitter peptides.
Therefore, the preparation method of the oligomeric peptide extract of abalone provided by the invention, by once hydrolyzing and secondary hydrolysis energy
Enough so that the protein in abalone meat is fully hydrolyzed as oligopeptide, and since a small amount of bitter peptides that a hydrolytic process generates exist
Hydrophobic amino acid is removed and makes in final product almost without bitter peptides in secondary hydrolysis, so that obtained oligopeptide
Extract it is in good taste.And finally obtained certain oligopeptides or compound fertilizer production can be with the reduced sugars in abalone meat
Maillard reaction, the mouthfeel of the extract advanced optimized occurs.
The present invention also provides a kind of methods preparing abalone oligomeric peptide extract and abalone powder comprising the present invention provides
The oligomeric peptide extract of abalone preparation method and meat slag procedure of processing, the meat slag procedure of processing be will be filtered in above-mentioned S4 steps
For obtained meat slag after washing at least twice, drying milling obtains abalone powder.
Although most of protein is decomposed in the abalone meat slag being obtained by filtration in S4 steps, it is also possible to deposit in meat slag
In the protein that some carbohydrates and esters and part are not decomposed, therefore it equally has higher nutritive value.
The present invention provides a kind of oligomeric peptide extracts of abalone, using the system of the oligomeric peptide extract of abalone provided by the invention
Preparation Method is made.
The oligomeric peptide extract of abalone provided by the invention can be used for preparing abalone oligopeptide piece.
The oligomeric peptide extract of abalone provided by the invention can be used for preparing abalone oligopeptide electuary.
Below in conjunction with specific embodiment to the method for the oligomeric peptide extract of preparation abalone provided by the invention and abalone powder into
Row illustrates.
Embodiment 1
Present embodiments provide a kind of method preparing the oligomeric peptide extract of abalone and abalone powder and by the method system
The abalone oligopeptide obtained.
A method of the oligomeric peptide extract of abalone and abalone powder are prepared, is included the following steps：
Fresh abalone was smashed into 40 mesh sieve, abalone meat gruel is made, and was then 0.7 in mass ratio by abalone meat gruel and water:1
It carries out mixing 10min and abalone meat gruel liquid is made；Abalone meat gruel liquid is placed in ball mill, control ball milling temperature is taken the photograph for -12
Family name's degree, ball milling frequency are 50HZ, Ball-milling Time 20min.
By the abalone meat gruel liquid after ball milling with mixing protease with mass ratio 1：0.6 is mixed, wherein mixing protease
It is 1 by mass ratio:0.8, vigor is that the alkali protease of 2500~2600U/g and trypsase are mixed to get, by egg mix
White enzyme is mixed evenly with abalone meat gruel liquid, then controls pH of mixed to 6~7, temperature is 40 DEG C, and enzymolysis 105min is obtained
First mixed enzymolysis liquid.
Compound fertilizer production, addition and the abalone meat gruel of compound fertilizer production are added into the first enzymolysis mixed liquor
The mass ratio of liquid is 0.8:1, after stirring evenly, it is 5.5~6.5 to adjust pH, and temperature is 45 DEG C, and it is mixed that reaction 60min obtains second
Enzymolysis liquid is closed, wherein the vigor for the compound fertilizer production being added is 900~1000U/g.
Second mixed enzymolysis liquid is filtered into removal meat slag by 60 mesh screens and obtains filtrate.
Filtrate is obtained into the oligomeric peptide extract of abalone by the substance that ultrafiltration retaining molecular weight is 80~1000 dalton,
Ultrafiltration concrete operations mode is as described in above-mentioned S5 steps.
Meat slag is dried into milling after washing twice and obtains abalone powder.
Embodiment 2
Present embodiments provide a kind of method preparing the oligomeric peptide extract of abalone and abalone powder and by the method system
The abalone oligopeptide obtained.
A method of the oligomeric peptide extract of abalone and abalone powder are prepared, is included the following steps：
Fresh abalone was smashed into 40 mesh sieve, abalone meat gruel is made, and was then 1 in mass ratio by abalone meat gruel and water:1 into
Row is mixed 10min and abalone meat gruel liquid is made；Abalone meat gruel liquid is placed in ball mill, control ball milling temperature is -8 Celsius
Degree, ball milling frequency are 80HZ, Ball-milling Time 120min.
By the abalone meat gruel liquid after ball milling with mixing protease with mass ratio 1：1 is mixed, wherein mixing protease by
Mass ratio is 1:1.2, vigor is that the alkali protease of 2500~2600U/g and trypsase are mixed to get, by mixed protein
Enzyme is mixed evenly with abalone meat gruel liquid, then controls pH of mixed to 6~7, and temperature is 50 DEG C, and enzymolysis 115min obtains the
One mixed enzymolysis liquid.
Compound fertilizer production, addition and the abalone meat gruel of compound fertilizer production are added into the first enzymolysis mixed liquor
The mass ratio of liquid is 1:1, after stirring evenly, it is 5.5~6.5 to adjust pH, and temperature is 55 DEG C, and reaction 70min obtains the second mixing
Enzymolysis liquid, wherein the vigor for the compound fertilizer production being added is 900~1000U/g.
Second mixed enzymolysis liquid is filtered into removal meat slag by 60 mesh screens and obtains filtrate.
Filtrate is obtained into the oligomeric peptide extract of abalone by the substance that ultrafiltration retaining molecular weight is 80~1000 dalton,
Ultrafiltration concrete operations mode is as described in above-mentioned S5 steps.
Meat slag is dried into milling after washing twice and obtains abalone powder.
Embodiment 3
Present embodiments provide a kind of method preparing the oligomeric peptide extract of abalone and abalone powder and by the method system
The abalone oligopeptide obtained.
A method of the oligomeric peptide extract of abalone and abalone powder are prepared, is included the following steps：
Fresh abalone was smashed into 40 mesh sieve, abalone meat gruel is made, and was then 0.8 in mass ratio by abalone meat gruel and water:1
It carries out mixing 10min and abalone meat gruel liquid is made；Abalone meat gruel liquid is placed in ball mill, control ball milling temperature is taken the photograph for -10
Family name's degree, ball milling frequency are 60HZ, Ball-milling Time 40min.
By the abalone meat gruel liquid after ball milling with mixing protease with mass ratio 1：0.7 is mixed, wherein mixing protease
It is 1 by mass ratio:0.9, vigor is that the alkali protease of 2500~2600U/g and trypsase are mixed to get, by egg mix
White enzyme is mixed evenly with abalone meat gruel liquid, then controls pH of mixed to 6~7, temperature is 45 DEG C, and enzymolysis 110min is obtained
First mixed enzymolysis liquid.
Compound fertilizer production, addition and the abalone meat gruel of compound fertilizer production are added into the first enzymolysis mixed liquor
The mass ratio of liquid is 0.9:1, after stirring evenly, it is 5.5~6.5 to adjust pH, and temperature is 50 DEG C, and it is mixed that reaction 65min obtains second
Enzymolysis liquid is closed, wherein the vigor for the compound fertilizer production being added is 900~1000U/g.
Second mixed enzymolysis liquid is filtered into removal meat slag by 60 mesh screens and obtains filtrate.
Filtrate is obtained into the oligomeric peptide extract of abalone by the substance that ultrafiltration retaining molecular weight is 80~1000 dalton,
Ultrafiltration concrete operations mode is as described in above-mentioned S5 steps.
Meat slag is dried into milling after washing twice and obtains abalone powder.
Embodiment 4
Present embodiments provide a kind of method preparing the oligomeric peptide extract of abalone and abalone powder and by the method system
The abalone oligopeptide obtained.
A method of the oligomeric peptide extract of abalone and abalone powder are prepared, is included the following steps：
Fresh abalone was smashed into 40 mesh sieve, abalone meat gruel is made, and was then 0.9 in mass ratio by abalone meat gruel and water:1
It carries out mixing 10min and abalone meat gruel liquid is made；Abalone meat gruel liquid is placed in ball mill, control ball milling temperature is taken the photograph for -11
Family name's degree, ball milling frequency are 70HZ, Ball-milling Time 70min.
By the abalone meat gruel liquid after ball milling with mixing protease with mass ratio 1：0.8 is mixed, wherein mixing protease
It is 1 by mass ratio:0.85, vigor is that the alkali protease of 2500~2600U/g and trypsase are mixed to get, by egg mix
White enzyme is mixed evenly with abalone meat gruel liquid, then controls pH of mixed to 6~7, temperature is 42 DEG C, and enzymolysis 108min is obtained
First mixed enzymolysis liquid.
Compound fertilizer production, addition and the abalone meat gruel of compound fertilizer production are added into the first enzymolysis mixed liquor
The mass ratio of liquid is 0.85:1, after stirring evenly, it is 5.5~6.5 to adjust pH, and temperature is 48 DEG C, and it is mixed that reaction 63min obtains second
Enzymolysis liquid is closed, wherein the vigor for the compound fertilizer production being added is 900~1000U/g.
Second mixed enzymolysis liquid is filtered into removal meat slag by 60 mesh screens and obtains filtrate.
Filtrate is obtained into the oligomeric peptide extract of abalone by the substance that ultrafiltration retaining molecular weight is 80~1000 dalton,
Ultrafiltration concrete operations mode is as described in above-mentioned S5 steps.
Meat slag is dried into milling after washing twice and obtains abalone powder.
Embodiment 5
Present embodiments provide a kind of method preparing the oligomeric peptide extract of abalone and abalone powder and by the method system
The abalone oligopeptide obtained.
A method of the oligomeric peptide extract of abalone and abalone powder are prepared, is included the following steps：
Fresh abalone was smashed into 40 mesh sieve, abalone meat gruel is made, and was then 0.85 in mass ratio by abalone meat gruel and water:1
It carries out mixing 10min and abalone meat gruel liquid is made；Abalone meat gruel liquid is placed in ball mill, control ball milling temperature is -9 Celsius
Degree, ball milling frequency are 65HZ, Ball-milling Time 100min.
By the abalone meat gruel liquid after ball milling with mixing protease with mass ratio 1：0.9 is mixed, wherein mixing protease
It is 1 by mass ratio:1.1, vigor is that the alkali protease of 2500~2600U/g and trypsase are mixed to get, by egg mix
White enzyme is mixed evenly with abalone meat gruel liquid, then controls pH of mixed to 6~7, temperature is 48 DEG C, and enzymolysis 112min is obtained
First mixed enzymolysis liquid.
Compound fertilizer production, addition and the abalone meat gruel of compound fertilizer production are added into the first enzymolysis mixed liquor
The mass ratio of liquid is 0.8:1, after stirring evenly, it is 5.5~6.5 to adjust pH, and temperature is 52 DEG C, and it is mixed that reaction 68min obtains second
Enzymolysis liquid is closed, wherein the vigor for the compound fertilizer production being added is 900~1000U/g.
Second mixed enzymolysis liquid is filtered into removal meat slag by 60 mesh screens and obtains filtrate.
Filtrate is obtained into the oligomeric peptide extract of abalone by the substance that ultrafiltration retaining molecular weight is 80~1000 dalton,
Ultrafiltration concrete operations mode is as described in above-mentioned S5 steps.
Meat slag is dried into milling after washing twice and obtains abalone powder.
In conclusion the preparation method of the oligomeric peptide extract of abalone provided by the invention, due to using by properly mixed
Alkali protease and trypsase are once hydrolyzed, and are carried out secondary hydrolysis using compound fertilizer production afterwards, are enabled to Bao
Protein in the flesh of fish is fully hydrolyzed as oligopeptide, and a small amount of bitter peptides generated due to a hydrolytic process are in secondary hydrolysis
Middle hydrophobic amino acid is removed and makes in final product almost without bitter peptides, so that obtained oligomeric peptide extract
It is in good taste, and beautiful draw can occur with the reduced sugar in abalone meat for finally obtained certain oligopeptides or compound fertilizer production
Moral is reacted, the mouthfeel of the oligomeric peptide extract of abalone advanced optimized.
The method provided by the invention for preparing the oligomeric peptide extract of abalone and abalone powder, due to including Bao provided by the invention
The oligomeric peptide extract of abalone provided by the invention can not only be made in the preparation method of fish oligopeptide extract, moreover it is possible to be made
The same abalone powder with nutritive value, abalone meat is fully fully utilized, is economized on resources.
The oligomeric peptide extract of abalone provided by the invention is easy to be inhaled by human body since it is almost all oligopeptide
It receives, there is higher nutritive value, and in good taste.
When abalone oligopeptide provided by the invention is used to prepare abalone oligopeptide piece so that in abalone oligopeptide piece obtained
Nutriment be easy to be absorbed.
When abalone oligopeptide provided by the invention is used to prepare abalone oligopeptide electuary so that abalone oligopeptide punching obtained
Nutriment in agent is easy to be absorbed.
The foregoing is merely the preferred embodiment of the present invention, are not intended to restrict the invention, for this field
For technical staff, the invention may be variously modified and varied.All within the spirits and principles of the present invention, any made by
Modification, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of preparation method of the oligomeric peptide extract of abalone, which is characterized in that preparation process includes：
Liquid processed：Abalone meat gruel and water are mixed to prepare abalone meat gruel liquid, the mass ratio of the abalone meat gruel and the water is 0.7~
1:1；
Primary enzymolysis：By the abalone meat gruel liquid and mixing protease with mass ratio 1:0.6~1 mixing, it is 7 to adjust pH of mixed
~8,105~115min of reaction obtain the first mixed enzymolysis liquid, and the mixing protease includes that mass ratio is 1:0.8~1.2 alkali
The vigor of property protease and trypsase, the alkali protease and the trypsase is 2500~2600U/g；
Secondary enzymolysis：Compound fertilizer production is added into first mixed enzymolysis liquid, the compound fertilizer production adds
The mass ratio for entering amount and the abalone meat gruel liquid is 0.8~1:1, it is 5.5~6.5 to adjust pH, and 60~70min of reaction obtains second
The vigor of mixed enzymolysis liquid, the compound fertilizer production is 900~1000U/g；
Filtering：Second mixed enzymolysis liquid is filtered into removal meat slag and obtains filtrate；
Ultrafiltration：Ultrafiltration retaining molecular weight is used to obtain the oligomeric peptide extract of abalone for the substance of 80~1000 dalton.
2. the preparation method of the oligomeric peptide extract of abalone according to claim 1, which is characterized in that by the abalone meat
Further include that abalone meat crushed 40 mesh to sieve to obtain the abalone meat gruel before Mi Yushui mixing.
3. the preparation method of the oligomeric peptide extract of abalone according to claim 1, which is characterized in that by the abalone meat gruel
Liquid further includes that the abalone meat gruel liquid is placed in mixing in ball mill before being mixed with the mixing protease.
4. the preparation method of the oligomeric peptide extract of abalone according to claim 3, which is characterized in that ball milling condition is temperature
Spend -12~-8 degrees Celsius, 50~80HZ of frequency, 20~120min of Ball-milling Time.
5. the preparation method of the oligomeric peptide extract of abalone according to claim 1, which is characterized in that in the primary enzymolysis
In the process, controlled enzymatic hydrolysis temperature is 40~50 DEG C.
6. the preparation method of the oligomeric peptide extract of abalone according to claim 1, which is characterized in that in the secondary enzymolysis
In the process, controlled enzymatic hydrolysis temperature is 45~55 DEG C.
7. a kind of method preparing abalone oligomeric peptide extract and abalone powder, which is characterized in that any including such as claim 1-6
The preparation method and meat slag procedure of processing of the oligomeric peptide extract of abalone described in, the meat slag procedure of processing are that will be obtained by filtration
The meat slag after washing at least twice, drying milling obtain abalone powder.
8. a kind of oligomeric peptide extract of abalone, which is characterized in that it uses abalone as claimed in any one of claims 1 to 6 oligomeric
The preparation method of peptide extract is made.
9. application of the oligomeric peptide extract of abalone according to claim 8 in preparing abalone oligopeptide piece.
10. application of the oligomeric peptide extract of abalone according to claim 8 in preparing abalone oligopeptide electuary.
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