CN114013827A - Self-sustaining liquid nitrogen type biological preservation container - Google Patents
Self-sustaining liquid nitrogen type biological preservation container Download PDFInfo
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- CN114013827A CN114013827A CN202111255171.5A CN202111255171A CN114013827A CN 114013827 A CN114013827 A CN 114013827A CN 202111255171 A CN202111255171 A CN 202111255171A CN 114013827 A CN114013827 A CN 114013827A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
- B65D25/38—Devices for discharging contents
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/18—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient
- B65D81/20—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient under vacuum or superatmospheric pressure, or in a special atmosphere, e.g. of inert gas
- B65D81/2069—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient under vacuum or superatmospheric pressure, or in a special atmosphere, e.g. of inert gas in a special atmosphere
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/38—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents with thermal insulation
- B65D81/3802—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents with thermal insulation rigid container in the form of a barrel or vat
- B65D81/3806—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents with thermal insulation rigid container in the form of a barrel or vat formed with double walls, i.e. hollow
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a self-maintaining liquid nitrogen type biological preservation container. The invention comprises a liquid nitrogen tank main body, a Stirling unit and a liquid nitrogen supply system, wherein the liquid nitrogen tank main body comprises a liquid nitrogen storage tank, an inner container sleeved outside the liquid nitrogen storage tank and an outer container sleeved outside the inner container, an object taking port for storing a biological sample is arranged in a space between the inner container and the outer container, a port of the Stirling unit is connected with the top end of the liquid nitrogen storage tank, the liquid nitrogen storage tank is connected with the liquid nitrogen supply system, a valve is arranged between the liquid nitrogen storage tank and the liquid nitrogen supply system, the Stirling unit is used for condensing volatilized nitrogen into liquid nitrogen, a storage tank exhaust connecting pipe for connecting the Stirling unit and the liquid nitrogen storage tank is provided with a pressure sensor, the inner container is provided with a temperature sensor, the pressure sensor and the temperature sensor are both connected with a controller, and the controller is connected with the valve. The invention breaks through the traditional refrigeration mode of long-term liquid nitrogen supply, and can realize the deep low-temperature storage environment of the liquid nitrogen by arranging an automatic liquid feeding mode of a liquid nitrogen supply tank.
Description
Technical Field
The invention relates to liquid nitrogen biological sample preservation, in particular to a self-maintaining liquid nitrogen type biological preservation container.
Background
The liquid nitrogen biological preservation container produced in the current market uses liquid nitrogen as a refrigeration source, and relies on low temperature brought by natural volatilization of the liquid nitrogen to maintain a deep low temperature environment in a tank, and the amount of the liquid nitrogen in the tank is gradually reduced along with the consumption of the liquid nitrogen, so that the liquid nitrogen needs to be supplied periodically. Some remote areas or high-end confidential scenes are inconvenient for regular supply of liquid nitrogen.
Liquid nitrogen tanks in the current market all need to depend on traditional liquid nitrogen supply tanks or pipelines to carry out the regular supply of liquid nitrogen, and the inconvenience brought by the method is as follows: 1. customers are required to have a long-term stable liquid nitrogen supplier; 2. remote areas have no liquid nitrogen supply channel; 3. the supply tank needs to be replaced frequently, and a large number of people frequently enter a sample management area; 4. the scientific research and security unit does not allow the entrance and exit of external personnel; 5. most of the liquid nitrogen supply tanks are pressure containers, and the liquid nitrogen supply tanks need to be recorded and registered, so that the liquid nitrogen supply tanks are not convenient to use; 6. the operators have risks of contacting with the liquid nitrogen, such as dangerous accidents like overflow of the liquid nitrogen; 7. the liquid feeding electromagnetic valve, the liquid level meter and the like are all quick-wear parts, and the failure rate is high. 8. For countries and regions with multiple earthquakes, the first energy source recovered after the earthquake is electric energy, so that a container for maintaining the temperature of liquid nitrogen by using electric energy is urgently expected
Disclosure of Invention
In accordance with the above-mentioned technical problems, a self-sustaining liquid nitrogen type biological preservation container is provided. The invention can realize the liquid nitrogen self-supply of the liquid nitrogen by depending on the electric energy (depending on the closed cycle refrigeration of the Stirling), not only can solve the pain point of a user end, but also breaks through the traditional refrigeration mode of long-term liquid nitrogen supply, realizes the innovation of the industry of the liquid nitrogen tank, and adds tiles for the high and new technology of China. The technical means adopted by the invention are as follows:
a self-maintaining liquid nitrogen type biological preservation container comprises a liquid nitrogen tank body, a Stirling machine set and a liquid nitrogen supply system, wherein the liquid nitrogen tank body comprises a liquid nitrogen storage tank, an inner container sleeved outside the liquid nitrogen storage tank and an outer container sleeved outside the inner container, an object taking port for preserving a biological sample is arranged in a space between the inner container and the outer container, the Stirling machine set is connected to the liquid nitrogen tank body through a flange, a port of the Stirling machine set is connected with the top end of the liquid nitrogen storage tank, the liquid nitrogen storage tank is connected with the liquid nitrogen supply system, a valve is arranged between the liquid nitrogen supply system and the liquid nitrogen supply system, the liquid nitrogen supply system is used for supplying a preset amount of liquid nitrogen into the liquid nitrogen storage tank, the Stirling machine set is used for condensing volatilized nitrogen into liquid nitrogen, a pressure sensor is arranged on a storage tank exhaust connecting pipe connected with the liquid nitrogen storage tank, and a temperature sensor is arranged on the inner container, the pressure sensor and the temperature sensor are both connected with a controller, the controller is connected with the valve and the Stirling unit, and the controller is used for controlling the opening and closing of the valve and the Stirling unit after judging that the liquid nitrogen allowance reaches a set value or the relation between the temperature value of the storage space and the set value.
Further, the outer container includes outer container barrel, outer container low head, outer container upper cover, truckle and inside and outside container support, the below of outer container barrel is connected outer container low head, and the top is connected outer container upper cover, the below of outer container low head is connected with the truckle, set up the inside and outside container support that is used for fixed inner container on the outer container low head.
Further, the inner and outer container supports comprise an outer container lower end socket supporting piece and a reinforcing rib plate connected with the outer container lower end socket supporting piece, the upper end of the outer container lower end socket supporting piece is connected with the inner lower end socket support, and a glass fiber reinforced plastic heat insulation layer is arranged between the upper end of the outer container lower end socket supporting piece and the inner lower end socket support.
Further, the inner container comprises an inner container barrel, an inner container lower end socket, an inner container upper end socket, a storage tank lower supporting piece, an inner rotating body and an inner rotating body, the inner container lower end socket is connected to the lower portion of the inner container barrel, the inner container upper end socket is connected to the upper portion of the inner container barrel, the inner container lower end socket is provided with the inner lower end socket inner rotating body supporting piece used for installing the inner rotating body, the inner lower end socket inner rotating body supporting piece is provided with the storage tank lower supporting piece used for fixing the liquid nitrogen storage tank, and the inner rotating body is connected to the outer portion of the liquid nitrogen storage tank through the inner rotating body.
Further, the valve is an electromagnetic valve, and when the temperature detected by the temperature sensor is not a set value, the liquid nitrogen replenishing system conveys liquid nitrogen into the liquid nitrogen storage tank by a self-pressurization principle; when the temperature detected by the temperature sensor reaches a set value, the electromagnetic valve is closed.
The Stirling unit comprises a cold head, a heat dissipation support plate, a Stirling engine, a heat dissipation fan, a radiator, knife edge flanges and a shock absorber, wherein the cold head is connected with the Stirling engine, the bottom end of the cold head is assembled with a liquid nitrogen tank body through the knife edge flanges, an oxygen-free copper gasket for sealing is arranged between the cold head and the liquid nitrogen tank body, the heat dissipation support plate is arranged in the space where the Stirling engine is located, the heat dissipation fan and the radiator are arranged on the heat dissipation support plate in preset numbers and preset positions, and the shock absorber is arranged between the two knife edge flanges.
Further, vacuum pumping treatment is carried out between the inner container and the outer container, a vacuum interlayer is arranged between the inner container and the outer container, a liquid adding pipe of the liquid nitrogen supply system is arranged in the area, and a vacuum plug is arranged on the outer container.
Further, the outer surface of the inner container is provided with a heat insulating material.
Furthermore, a pressure detection port where the pressure sensor is located is connected with a safety valve communicated with the outside, when the pressure of the liquid nitrogen storage tank is too high, the safety valve is automatically opened to protect the tank body, and when the pressure is released to a safety value, the safety valve is automatically closed.
Furthermore, a liquid adding pipe of the liquid nitrogen supply system is also connected with a manual liquid adding port.
The invention has the following advantages:
1. for remote areas such as inconvenient liquid nitrogen transportation, the refrigeration of the liquid nitrogen can be realized only by adding the liquid nitrogen and long-term electric energy at one time, and a lower and more stable deep low temperature storage scene is provided.
2. The waiting time for filling and transporting of the liquid nitrogen replenishing tank is not needed, and manpower and material resources are saved.
3. The double-selection possibility is provided, and the liquid nitrogen deep low-temperature storage environment can be realized by the automatic liquid adding mode of the liquid nitrogen supply tank in the scene of insufficient electric energy and convenient liquid nitrogen transportation.
4. The invention has reliable structure, can be applied to other environments using liquid nitrogen for refrigeration, such as the fields of aviation detectors, unmanned planes and the like, and conforms to the concept of intelligent development.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic view of the external structure of the present invention.
Fig. 2 is a sectional view of a front view of the internal structure of the present invention.
Fig. 3 is a sectional view taken along line a-a of fig. 2.
FIG. 4 is a top view of the present invention.
Fig. 5 is a cross-sectional view B-B of fig. 2.
In the figure: 111. supporting an inner lower end enclosure; 112. a lower end enclosure of the inner container; 113. a support member for the inner rotary body in the inner lower end socket; 12. an inner container cylinder; 131. a storage tank lower support; 132. a tank barrel; 133. a storage tank exhaust connecting pipe; 141. an inner rotary body outer cylinder; 142. an inner rotary body lower rotary member; 143. the inner rotary body is provided with a rotary piece; 144. An inner barrel of the inner rotary body; 145. an inner rotary body partition plate; 151. an upper end socket of the inner container; 152. the object taking opening is contracted; 153. an article taking opening; 154. the object taking opening is contracted; 155. a fetching port cover; 156. a sleeve; 157. A knife edge flange; 211. a glass fiber reinforced plastic heat insulation layer; 212. a lower end enclosure support of the outer container; 213. reinforcing rib plates; 214. a caster wheel; 215. a lower end enclosure of the outer container; 22. vacuum plugging; 23. an upper end enclosure of the outer container; 24. an outer container cylinder; 25. a thermally insulating material; 26. vacuum interlayer; 31. cooling the head; 32. a heat dissipation support plate; 33. stirling machine (model TC 4187); 34. a fan; 35. a heat sink; 36. a knife edge flange; 37. a shock absorber; 38. an oxygen-free copper washer; 41. a temperature sensor assembly; 42. a pressure sensor assembly; 421. a pressure detection port; 422. a pipe clamp; 423. a pressure sensor; 424. a safety valve; 43. A manual liquid feeding port; 431. a liquid feeding pipe; 432. a stop valve; 433. a liquid feeding pipe clamp; 434. a liquid filling port; 5. a controller (model CT 5505).
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1 to 4, the embodiment of the invention discloses a self-sustaining liquid nitrogen type biological preservation container, which comprises a liquid nitrogen tank main body, a stirling machine set and a liquid nitrogen replenishment system, wherein the liquid nitrogen tank main body comprises a liquid nitrogen storage tank, an inner container sleeved outside the liquid nitrogen storage tank and an outer container sleeved outside the inner container, an extraction opening for preserving a biological sample is arranged in a space between the inner container and the outer container, the stirling machine set is connected to the liquid nitrogen tank main body through a flange, a port of the stirling machine set is connected with the top end of the liquid nitrogen storage tank, the liquid nitrogen storage tank is connected with the liquid nitrogen replenishment system, a valve is arranged between the liquid nitrogen replenishment system and the liquid nitrogen replenishment system, the liquid nitrogen replenishment system is used for supplying a preset amount of liquid nitrogen into the liquid nitrogen storage tank, the stirling machine set is used for condensing volatilized nitrogen into liquid nitrogen, a storage tank exhaust connecting pipe 133 connected with the stirling machine set and the liquid nitrogen storage tank is provided with a pressure sensor 423, and a temperature sensor 41 is arranged on the inner container, the pressure sensor and the temperature sensor are both connected with a controller 5, the controller is connected with the valve, and the controller is also connected with the Stirling unit and used for controlling the opening and closing of the valve and the Stirling unit after judging that the residual liquid nitrogen amount reaches a set value or the relation between the temperature value of the storage space and the set value. The upper end socket of the inner container 151 is welded with the lower necking 152 of the fetching port, the fetching port 153, the upper necking 154 of the fetching port, the cover 155 of the fetching port, the sleeve 156 and the knife-edge flange 157 to form an upper end socket assembly.
The outer container includes outer container barrel 24, outer container low head 215, outer container upper cover 23, truckle 214 and inside and outside container support, the below of outer container barrel is connected outer container low head, and the top is connected outer container upper cover, the below of outer container low head is connected with the truckle, set up the inside and outside container support that is used for fixed inner container on the outer container low head. The inner and outer container supports comprise an outer container lower end socket support part 212 and a reinforcing rib plate 213 connected with the outer container lower end socket support part, the upper end of the outer container lower end socket support part is connected with the inner lower end socket support 111, and a glass fiber reinforced plastic heat insulation layer 211 is arranged between the upper end socket support part and the inner lower end socket support part.
The inner container comprises an inner container barrel 12, an inner container lower end socket 112, an inner container upper end socket 151, a storage tank lower supporting piece 131, an inner rotating body and an inner rotating body, the inner container lower end socket is connected below the inner container barrel, the inner container upper end socket is connected above the inner container barrel, an inner lower end socket inner rotating body supporting piece 113 used for installing the inner rotating body is arranged on the inner container lower end socket, a storage tank lower supporting piece used for fixing the liquid nitrogen storage tank 132 is arranged on the inner lower end socket inner rotating body supporting piece, the inner rotating body comprises an inner rotating body inner barrel 144 and an inner rotating body outer barrel 141, the inner rotating body inner barrel 144 is connected to the outside of the liquid nitrogen storage tank through an inner rotating body upper rotating piece 143 and an inner rotating body lower rotating piece 142, and the inner rotating body can be manually rotated by taking a sleeve 156 as a center. The inner rotor is in the form of a circular ring in plan view (layout view), see fig. 5, and in this embodiment, the area between the inner rotor inner cylinder and the inner rotor outer cylinder is divided into 5 areas (A, B, C, D, E) by 5 inner rotor partitions 145, and area identifications are made on the partitions, each of which can store a biological sample individually. When the warehouse entry operation is carried out, the object taking opening cover 155 is opened, a hand extends into the inner rotary body to stir the partition plate 145 until a storage area is found, the warehouse entry operation is carried out, the cover plate is covered after the operation is finished, and in other optional embodiments, the number of the partition plates 145 of the inner rotary body is set according to requirements. The inner lower seal head support 111, the inner lower seal head 112 and the inner lower seal head inner body support member are assembled and welded into an inner container lower seal head assembly; the lower support 131, the cylinder 132 and the exhaust pipe are combined to form a liquid nitrogen storage tank assembly.
The liquid nitrogen storage tank assembly is placed on the inner container lower end socket assembly and welded, and then the inner container barrel body 12 and the inner rotating body are assembled, so that the inner rotating body can rotate freely; and installing the inner upper end socket assembly, completing welding, cleaning the outer surface, winding the heat insulation material on the outer surface of the inner container to form a physical heat insulation layer, and forming the inner container.
The liquid nitrogen replenishing system comprises a liquid feeding pipe 431 and a valve connected to the liquid feeding pipe, the valve is an electromagnetic valve, specifically is a stop valve 432, the liquid feeding pipe is fixed at a preset position at the top of the outer container through a liquid feeding pipe clamp 433, and the end of the liquid feeding pipe is a liquid feeding port 434.
When the temperature detected by the temperature sensor is not a set value, the liquid nitrogen supply system conveys liquid nitrogen to a liquid nitrogen storage tank by a self-pressurization principle; when the temperature detected by the temperature sensor reaches a set value, the electromagnetic valve is closed.
The Stirling unit comprises a cold head 31, a heat dissipation support plate 32, a Stirling engine 33, a heat dissipation fan 34, a heat radiator 35, a knife edge flange 36 and a shock absorber 37, wherein the cold head is connected with the Stirling engine, the bottom end of the cold head is assembled with a liquid nitrogen tank body through the knife edge flange, an oxygen-free copper gasket 38 for sealing is arranged between the cold head and the liquid nitrogen tank body, the heat dissipation support plate is arranged in the space where the Stirling engine is located, the heat dissipation fan and the heat radiator are arranged on the heat dissipation support plate in preset quantity and preset positions, the left axial flow fan 34 and the right axial flow fan 34 are respectively arranged on the left side and the right side of the heat dissipation support plate for air cooling of heat brought by piston movement of the Stirling engine, the heat radiator 35 is specifically a fin radiator (piece 35), and fins are heat exchange surfaces and are used for increasing heat dissipation of the Stirling engine so as to maintain the upper temperature within a reasonable range. The radiator is composed of a clamping copper block, a heat pipe and an aluminum fin. Before the clamping copper block is matched with the hot end copper block of the low-temperature refrigerator, a layer of heat-conducting paste needs to be uniformly coated on the inner circular surface of the clamping copper block so as to reduce the heat-conducting resistance between the clamping copper block and the inner circular surface, and then the clamping copper block is locked by four locking screws of the clamping copper block.
Set up between two edge of a knife flanges the bumper shock absorber, the bumper shock absorber is expansion joint formula bellows bumper shock absorber, connects jar body and stirling, and when stirling refrigerator during operation, the compressor will arouse certain vibrations, and this amplitude is given for the bumper shock absorber, and the bellows on the bumper shock absorber will take place elastic deformation, offsets the vibrations that the stirling arouses to the biological sample of storage does not receive the influence of stirling vibrations in the protection jar. The two butt flanges are fastened through bolts, flat gaskets, elastic gaskets and nuts, and simultaneously, knife edge surfaces of the flanges enter the oxygen-free copper gaskets to form sealing surfaces, and finally, a sealing space of the Stirling liquid nitrogen tank is formed.
As an alternative embodiment, the stirling cryocooler used in this embodiment is model TC4187, which is a pulse tube type cryocooler with two opposite motors, and the cryocooler has no moving parts except a motor piston, and has the advantages of fewer moving parts and low mechanical failure rate compared to the conventional stirling cryocooler, and the advantages of small vibration due to the mutual cancellation of the opposite motor vibrations. The low-temperature refrigerator consists of the following parts: the cold finger, two opposite linear compressors, an air reservoir and other parts. The two opposite linear compressors are supported by air floatation, the piston and the cylinder do not directly contact when moving linearly relatively, an air film between the piston and the cylinder serves as an air bearing to play an air floatation role, and the design can effectively prolong the service life of a product. Sufficient piston displacement is the key for ensuring the air flotation effect, and the condition for determining the piston displacement is the input voltage of the refrigerator, so that the service life of a product can be seriously influenced by long-term operation below the minimum voltage for the TC4187 low-temperature refrigerator, and the sufficient operating voltage is an important factor for ensuring the service life of the product.
Therefore, the present invention requires strict control of the input voltage, so CT5505 is selected as the controller. The controller can provide stable 24V power supply input for the TC4187 Stirling refrigerator, and automatically adjusts the input power of the TC4187 Stirling refrigerator after comparing the monitored cold head temperature with the target temperature in a temperature control mode; meanwhile, the temperature of the hot end is monitored, when the monitored temperature exceeds the range, an alarm is automatically given or the refrigerator is automatically shut down, and the refrigerator is prevented from being damaged unrecoverably.
The outer surface of the inner container is provided with a thermally insulating material 25.
And a liquid adding pipe of the liquid nitrogen replenishing system is also connected with a manual liquid adding port 43.
Before use, the space between the inner container and the outer container needs to be vacuumized, a liquid feeding pipe of the liquid nitrogen supply system is arranged in the area, and a vacuum plug 22 is arranged on the outer container. Specifically, in the installation process, the inner container is placed in the lower end enclosure assembly of the outer container, then the outer container barrel 24, the vacuum plug 22, the upper end enclosure 23 of the outer container, the temperature sensor assembly 41, the pressure sensor assembly 42 and the manual liquid adding port 43 are respectively assembled and welded, a helium mass spectrometer leak detector is connected, and helium leak detection is carried out on the interlayer, wherein the required leak rate is less than or equal to 1.0 × 10-9Pa.m3And/s, after the leakage detection is qualified, opening the vacuum plug, and vacuumizing the tank body by using a vacuum unit to reach 2 x 10-5Pa to form a vacuum insulation sandwich 26 and finally form a finished can body.
The specific use method of the invention is as follows:
through filling opening and liquid nitrogen supply system connection, open the stop valve, liquid nitrogen container will be through from the automatic filling of pressure boost principle to the liquid nitrogen storage tank sub-assembly in, the liquid level rises gradually, and the temperature descends gradually simultaneously, through storage tank barrel jar wall cold and hot exchange, with cold volume transmission to the storage space of adversion body in to transmit the temperature to the controller through the temperature sensor subassembly and show, after the temperature reached the setting value, close the stop valve.
Because natural heat transfer, the liquid nitrogen can last volatilizing, and 680L's nitrogen gas can be volatilized to 1L's liquid nitrogen, and along with the liquid nitrogen sublimation becomes nitrogen gas, the gas pressure in the liquid nitrogen storage tank will increase, can convert out the surplus of liquid nitrogen through the atmospheric pressure value to transmit the liquid level value to the controller in through the pressure sensor subassembly, the liquid nitrogen surplus in the real time monitoring jar.
The nitrogen in the storage tank naturally flows upwards, is condensed into liquid nitrogen (Stirling refrigeration technology) when reaching a Stirling cold head, and drops back into the liquid nitrogen storage tank; finally, a closed circulation of liquid nitrogen is formed.
When the residual liquid nitrogen amount is lower than a set value or the temperature value of the storage space is higher than the set value, the controller controls the Stirling refrigerating unit to start working, and when the residual liquid nitrogen amount reaches the set value or the temperature value of the storage space is lower than the set value, the controller controls the Stirling refrigerating unit to stop working.
When the pressure of the liquid nitrogen storage tank is too high, the safety valve (spring full-open type) is automatically opened to protect the tank body, and when the pressure is released to a safety value, the safety valve is automatically closed.
When the Stirling unit breaks down or the electric energy is insufficient, a manual liquid filling port can be adopted for manual liquid supplement, and double protection is performed.
The tank body can be pushed and locked freely by using casters.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (10)
1. A self-maintaining liquid nitrogen type biological preservation container is characterized by comprising a liquid nitrogen tank main body, a Stirling unit and a liquid nitrogen supply system, wherein the liquid nitrogen tank main body comprises a liquid nitrogen storage tank, an inner container sleeved outside the liquid nitrogen storage tank and an outer container sleeved outside the inner container, an object taking port for preserving a biological sample is arranged in a space between the inner container and the outer container, the Stirling unit is connected to the liquid nitrogen tank main body through a flange, a port of the Stirling unit is connected with the top end of the liquid nitrogen storage tank, the liquid nitrogen storage tank is connected with the liquid nitrogen supply system, a valve is arranged between the liquid nitrogen storage tank and the liquid nitrogen supply system, the liquid nitrogen supply system is used for supplying a preset amount of liquid nitrogen into the liquid nitrogen storage tank, the Stirling unit is used for condensing volatile nitrogen into liquid nitrogen, a pressure sensor is arranged on an exhaust connecting pipe of the storage tank, which is connected with the liquid nitrogen storage tank, and a temperature sensor is arranged on the inner container, the pressure sensor and the temperature sensor are both connected with a controller, the controller is connected with the valve, and the controller is used for controlling the opening and closing of the valve and the Stirling unit after judging that the liquid nitrogen allowance reaches a set value or the relation between the temperature value of the storage space and the set value.
2. The self-sustaining liquid nitrogen-type biological preservation container according to claim 1, wherein the outer container comprises an outer container cylinder, an outer container lower end enclosure, an outer container upper end enclosure, casters and inner and outer container supports, the outer container cylinder is connected with the outer container lower end enclosure at the lower part and connected with the outer container upper end enclosure at the upper part, the casters are connected with the outer container lower end enclosure at the lower part, and the inner and outer container supports for fixing the inner container are arranged on the outer container lower end enclosure.
3. The self-sustaining liquid nitrogen-type biological preservation container according to claim 2, wherein the inner and outer container supports comprise an outer container lower head support and a reinforcing rib plate connected with the outer container lower head support, the upper end of the outer container lower head support is connected with the inner lower head support, and a glass fiber reinforced plastic heat insulation layer is arranged between the outer container lower head support and the inner lower head support.
4. The self-sustaining liquid nitrogen-type biological storage container according to claim 1 or 2, wherein the inner container comprises an inner container cylinder, an inner container lower head, an inner container upper head, a storage tank lower support, an inner rotating member and an inner rotating member, the inner container cylinder is connected with the inner container lower head at the lower part and connected with the inner container upper head at the upper part, the inner container lower head is provided with an inner lower head inner rotating member support for mounting the inner rotating member, the inner lower head inner rotating member support is provided with a storage tank lower support for fixing the liquid nitrogen storage tank, and the inner rotating member is connected with the outside of the liquid nitrogen storage tank through the inner rotating member.
5. The self-sustaining liquid nitrogen-type biological storage container according to claim 1, wherein said valve is a solenoid valve, and said liquid nitrogen replenishment system delivers liquid nitrogen to the liquid nitrogen storage tank by a self-pressurization principle when the temperature detected by the temperature sensor is not a set value; when the temperature detected by the temperature sensor reaches a set value, the electromagnetic valve is closed.
6. The self-sustaining liquid nitrogen-type biological storage container according to claim 1, wherein the stirling machine set comprises a cold head, a heat dissipation support plate, a stirling machine, a heat dissipation fan, a heat sink, a knife-edge flange and a shock absorber, wherein the cold head is connected with the stirling machine, the bottom end of the cold head is assembled with the liquid nitrogen tank body through the knife-edge flange, an oxygen-free copper gasket for sealing is arranged between the cold head and the liquid nitrogen tank body, the heat dissipation support plate is arranged in a space where the stirling machine is located, the heat dissipation fan and the heat sink are arranged on the heat dissipation support plate in preset numbers and preset positions, and the shock absorber is arranged between the two knife-edge flanges.
7. The self-sustaining liquid nitrogen-type bio-preservation container according to claim 1, wherein a vacuum is applied between the inner container and the outer container, a vacuum interlayer is interposed between the inner container and the outer container, a liquid feeding tube of the liquid nitrogen replenishment system is disposed in the area, and a vacuum block is disposed on the outer container.
8. The self-sustaining liquid nitrogen-based bioprotective container of claim 1, wherein an outer surface of the inner container is provided with a thermal insulation material.
9. The self-sustaining liquid nitrogen type bio-preservation container according to claim 1, wherein the pressure detection port of the pressure sensor is connected to a safety valve connected to the outside, and when the pressure of the liquid nitrogen storage tank is too high, the safety valve is automatically opened to protect the tank body, and when the pressure is released to a safe value, the safety valve is automatically closed.
10. The self-sustaining liquid nitrogen-based biological storage container of claim 1 or 7, wherein a manual fill port is further connected to the fill tube of the liquid nitrogen replenishment system.
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