CN114009632A - Method for preparing biological medicine fish feed from biogas slurry and hydrolyzed organic matter and fish feed - Google Patents
Method for preparing biological medicine fish feed from biogas slurry and hydrolyzed organic matter and fish feed Download PDFInfo
- Publication number
- CN114009632A CN114009632A CN202111396852.3A CN202111396852A CN114009632A CN 114009632 A CN114009632 A CN 114009632A CN 202111396852 A CN202111396852 A CN 202111396852A CN 114009632 A CN114009632 A CN 114009632A
- Authority
- CN
- China
- Prior art keywords
- feed
- fish feed
- biogas slurry
- fish
- organic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000251468 Actinopterygii Species 0.000 title claims abstract description 100
- 239000002002 slurry Substances 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims abstract description 28
- 239000003814 drug Substances 0.000 title claims abstract description 16
- 239000005416 organic matter Substances 0.000 title claims description 7
- 239000002028 Biomass Substances 0.000 claims abstract description 35
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 28
- 244000005700 microbiome Species 0.000 claims abstract description 19
- 238000000354 decomposition reaction Methods 0.000 claims abstract description 15
- 235000011837 pasties Nutrition 0.000 claims abstract description 12
- 102000004190 Enzymes Human genes 0.000 claims abstract description 11
- 108090000790 Enzymes Proteins 0.000 claims abstract description 11
- 238000001035 drying Methods 0.000 claims abstract description 11
- 230000000813 microbial effect Effects 0.000 claims abstract description 11
- 238000002156 mixing Methods 0.000 claims abstract description 6
- 238000012258 culturing Methods 0.000 claims abstract description 4
- 238000003756 stirring Methods 0.000 claims abstract description 4
- 210000003608 fece Anatomy 0.000 claims description 15
- 239000010806 kitchen waste Substances 0.000 claims description 15
- 239000010871 livestock manure Substances 0.000 claims description 14
- 230000029087 digestion Effects 0.000 claims description 12
- 206010024380 Leukoderma Diseases 0.000 claims description 11
- 229940088598 enzyme Drugs 0.000 claims description 10
- 235000012055 fruits and vegetables Nutrition 0.000 claims description 10
- 239000002699 waste material Substances 0.000 claims description 9
- 241000186660 Lactobacillus Species 0.000 claims description 7
- 241000283690 Bos taurus Species 0.000 claims description 6
- 241000287828 Gallus gallus Species 0.000 claims description 6
- 241001494479 Pecora Species 0.000 claims description 6
- 239000010802 sludge Substances 0.000 claims description 6
- 239000004382 Amylase Substances 0.000 claims description 5
- 102000013142 Amylases Human genes 0.000 claims description 5
- 108010065511 Amylases Proteins 0.000 claims description 5
- 101710130006 Beta-glucanase Proteins 0.000 claims description 5
- 241000186000 Bifidobacterium Species 0.000 claims description 5
- 108010059892 Cellulase Proteins 0.000 claims description 5
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 claims description 5
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 5
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 5
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 claims description 5
- 108091005804 Peptidases Proteins 0.000 claims description 5
- 108010059820 Polygalacturonase Proteins 0.000 claims description 5
- 239000004365 Protease Substances 0.000 claims description 5
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 5
- 241000194017 Streptococcus Species 0.000 claims description 5
- 235000019418 amylase Nutrition 0.000 claims description 5
- 229940106157 cellulase Drugs 0.000 claims description 5
- 150000001875 compounds Chemical class 0.000 claims description 5
- 108010093305 exopolygalacturonase Proteins 0.000 claims description 5
- 229940039696 lactobacillus Drugs 0.000 claims description 5
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 5
- 229940004208 lactobacillus bulgaricus Drugs 0.000 claims description 5
- 238000002791 soaking Methods 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- 241000283086 Equidae Species 0.000 claims description 2
- 239000008187 granular material Substances 0.000 claims description 2
- 241000272517 Anseriformes Species 0.000 claims 2
- 241000186672 Lactobacillus delbrueckii subsp. bulgaricus Species 0.000 claims 1
- 241000282887 Suidae Species 0.000 claims 1
- 235000013330 chicken meat Nutrition 0.000 claims 1
- 201000010099 disease Diseases 0.000 abstract description 8
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 8
- 239000002994 raw material Substances 0.000 abstract description 6
- 238000009360 aquaculture Methods 0.000 abstract description 5
- 244000144974 aquaculture Species 0.000 abstract description 5
- 206010035148 Plague Diseases 0.000 abstract description 4
- 241000607479 Yersinia pestis Species 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 239000008188 pellet Substances 0.000 abstract 1
- 230000002265 prevention Effects 0.000 abstract 1
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 16
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 12
- 241000894006 Bacteria Species 0.000 description 11
- 235000013311 vegetables Nutrition 0.000 description 7
- 241001596950 Larimichthys crocea Species 0.000 description 6
- 235000013399 edible fruits Nutrition 0.000 description 6
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 6
- 235000013372 meat Nutrition 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 241000272525 Anas platyrhynchos Species 0.000 description 5
- 239000010813 municipal solid waste Substances 0.000 description 5
- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 description 4
- 229920002498 Beta-glucan Polymers 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 4
- 244000199885 Lactobacillus bulgaricus Species 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 4
- UGXQOOQUZRUVSS-ZZXKWVIFSA-N [5-[3,5-dihydroxy-2-(1,3,4-trihydroxy-5-oxopentan-2-yl)oxyoxan-4-yl]oxy-3,4-dihydroxyoxolan-2-yl]methyl (e)-3-(4-hydroxyphenyl)prop-2-enoate Chemical compound OC1C(OC(CO)C(O)C(O)C=O)OCC(O)C1OC1C(O)C(O)C(COC(=O)\C=C\C=2C=CC(O)=CC=2)O1 UGXQOOQUZRUVSS-ZZXKWVIFSA-N 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 4
- 230000000433 anti-nutritional effect Effects 0.000 description 4
- 229920000617 arabinoxylan Polymers 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 4
- 239000002131 composite material Substances 0.000 description 4
- 230000008030 elimination Effects 0.000 description 4
- 238000003379 elimination reaction Methods 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 230000009545 invasion Effects 0.000 description 4
- 235000021049 nutrient content Nutrition 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 4
- 241000272814 Anser sp. Species 0.000 description 3
- 241000283073 Equus caballus Species 0.000 description 3
- 241000282898 Sus scrofa Species 0.000 description 3
- 239000004310 lactic acid Substances 0.000 description 3
- 235000014655 lactic acid Nutrition 0.000 description 3
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 231100000699 Bacterial toxin Toxicity 0.000 description 2
- 108010062877 Bacteriocins Proteins 0.000 description 2
- 108010031186 Glycoside Hydrolases Proteins 0.000 description 2
- 102000005744 Glycoside Hydrolases Human genes 0.000 description 2
- 108010053775 Nisin Proteins 0.000 description 2
- NVNLLIYOARQCIX-MSHCCFNRSA-N Nisin Chemical compound N1C(=O)[C@@H](CC(C)C)NC(=O)C(=C)NC(=O)[C@@H]([C@H](C)CC)NC(=O)[C@@H](NC(=O)C(=C/C)/NC(=O)[C@H](N)[C@H](C)CC)CSC[C@@H]1C(=O)N[C@@H]1C(=O)N2CCC[C@@H]2C(=O)NCC(=O)N[C@@H](C(=O)N[C@H](CCCCN)C(=O)N[C@@H]2C(NCC(=O)N[C@H](C)C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCSC)C(=O)NCC(=O)N[C@H](CS[C@@H]2C)C(=O)N[C@H](CC(N)=O)C(=O)N[C@H](CCSC)C(=O)N[C@H](CCCCN)C(=O)N[C@@H]2C(N[C@H](C)C(=O)N[C@@H]3C(=O)N[C@@H](C(N[C@H](CC=4NC=NC=4)C(=O)N[C@H](CS[C@@H]3C)C(=O)N[C@H](CO)C(=O)N[C@H]([C@H](C)CC)C(=O)N[C@H](CC=3NC=NC=3)C(=O)N[C@H](C(C)C)C(=O)NC(=C)C(=O)N[C@H](CCCCN)C(O)=O)=O)CS[C@@H]2C)=O)=O)CS[C@@H]1C NVNLLIYOARQCIX-MSHCCFNRSA-N 0.000 description 2
- 241000209504 Poaceae Species 0.000 description 2
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 2
- 241000191025 Rhodobacter Species 0.000 description 2
- 241000607142 Salmonella Species 0.000 description 2
- 241000607768 Shigella Species 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 238000012271 agricultural production Methods 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 239000000688 bacterial toxin Substances 0.000 description 2
- 239000003738 black carbon Substances 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 235000013601 eggs Nutrition 0.000 description 2
- 210000002919 epithelial cell Anatomy 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000010794 food waste Substances 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 239000004021 humic acid Substances 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 239000004309 nisin Substances 0.000 description 2
- 235000010297 nisin Nutrition 0.000 description 2
- 235000013348 organic food Nutrition 0.000 description 2
- 239000001814 pectin Substances 0.000 description 2
- 229920001277 pectin Polymers 0.000 description 2
- 235000010987 pectin Nutrition 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000010865 sewage Substances 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000003053 toxin Substances 0.000 description 2
- 231100000765 toxin Toxicity 0.000 description 2
- 108700012359 toxins Proteins 0.000 description 2
- 235000019786 weight gain Nutrition 0.000 description 2
- 241000383620 Allium mongolicum Species 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 240000004658 Medicago sativa Species 0.000 description 1
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 235000021190 leftovers Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/123—Bulgaricus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Animal Husbandry (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Insects & Arthropods (AREA)
- Marine Sciences & Fisheries (AREA)
- Birds (AREA)
- Botany (AREA)
- Mycology (AREA)
- Sustainable Development (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a method for preparing biological medicine fish feed by biogas slurry and hydrolyzed organic matters, which comprises the following steps: s1: adding complex enzyme and complex microorganism into anaerobically digested biogas slurry, and culturing for 24-120 h; obtaining an aqueous microbial feed; s2: the organic biomass is sent into a decomposition tank for decomposition, after the decomposition is finished, the organic biomass is treated for 0.5 to 10 hours under the conditions of 1.2MPa and 200 ℃, and then dried to obtain dried organic biomass with the water content of less than 20 percent; s3: drying the organic biomass and the aqueous microbial feed according to a ratio of 1: 0.5-3 weight ratio, and mixing and stirring into pasty feed; s4: and drying the pasty feed at normal temperature, and granulating when the water content of the pasty feed is 37-45% to obtain the granular fish feed. According to the invention, through the combination of various process methods and the refined selection of raw materials, the production of the water-based microbial feed and the pellet fish feed with the biological medicine function by taking the biogas slurry and the organic biomass as the raw materials is realized, and the prevention and treatment of aquaculture diseases and plagues are solved.
Description
Technical Field
The invention relates to the technical field of fish feed, in particular to a method for preparing biological medicine fish feed by biogas slurry and hydrolyzed organic matters and fish feed.
Background
The culture excrement or the fermented biogas residue and biogas slurry can be used as feed for fish culture in the pond, which is a well-known thing for most farmers in rural areas, and the water in the pond needs to be replaced regularly to prevent the virus of the fish leukoderma from multiplying and diffusing to cause large-area death of the pond.
The marine product cultivation of marine ranch has the same thing, when the cultivation area increases, breed density increases, can produce a large amount of leukoderma in the cultivation district, and this kind of leukoderma spreads the speed extremely fast, will cause the death of large tracts of land marine product in half a month generally appearing, and for marine ranch, this is the disaster, both causes fisherman's major economic loss, and direct pollution marine environment again, and after the sick and plague takes place, the use value has been lost temporarily in the area sea field of morbidity.
Accordingly, the prior art is deficient and needs improvement.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: provides a method for preparing biological medicine fish feed by biogas slurry and hydrolyzed organic matters and fish feed, which makes full use of breeding excrement resources and is used for eliminating fish leukoderma.
The technical scheme of the invention is as follows: the method for preparing the biological medicine fish feed by the biogas slurry and the hydrolyzed organic matters comprises the following steps.
S1: adding complex enzyme and complex microorganism into anaerobically digested biogas slurry, and culturing for 24-120 h; obtaining the water-based microbial feed.
S2: and (3) the organic biomass is sent into a decomposition tank for decomposition, and after the decomposition is finished, the organic biomass is treated for 0.5 to 10 hours under the conditions of 1.2MPa and 200 ℃, and then dried to obtain the dried organic biomass with the water content of less than 20 percent. The high temperature and high pressure can eliminate all ova, mixed bacteria and various harmful residues in the organic biomass, and high-quality dry organic biomass is produced.
S3: drying the organic biomass and the aqueous microbial feed according to a ratio of 1: 0.5-3 weight ratio, and mixing and stirring to obtain pasty feed.
S4: and drying the pasty feed at normal temperature, and granulating when the water content of the pasty feed is 37-45% to obtain the granular fish feed.
The method for preparing the biological medicine fish feed by the biogas slurry and the hydrolyzed organic matters further comprises the step S5: continuously drying the granulated fish feed at normal temperature to ensure that the water content of the granulated fish feed is lower than 35 percent, thereby obtaining the dried granulated fish feed; the density of the dried fish feed granules is 0.6-0.8g/cm3The density after soaking in water is 0.93-0.99g/cm3. The density of the granulated fish feed after soaking in water is less than that of water, so the granulated fish feed can float on the water surface and is convenient for fish to eat.
The compound enzyme comprises: at least two of protease, amylase, pectinase, cellulase, xylanase and beta-glucanase.
The complex enzyme can effectively decompose anti-nutritional factors in the fish feed, release nutrient substances, enable the nutrient substances to be fully digested and absorbed, improve the utilization rate of the fish feed and improve the production performance of animals. The xylanase effectively degrades the arabinoxylan in the fish feed, eliminates the anti-nutritional effect of the arabinoxylan on animals, and improves the utilization rate of fish feed resources. Beta-glucanase: eliminates the harm of beta-glucan in the fish feed, degrades the beta-glucan into absorbable reducing sugar and improves the nutritional value. Cellulase: cellulose in the fish feed is hydrolyzed into glucose, so that the utilization rate of the fish feed is greatly improved. Protease: the protein in the fish feed is hydrolyzed, which is beneficial to the absorption of the fish. Amylase: the starch in the fish feed is decomposed into glucose, which is beneficial to the absorption of the fish. And (3) pectinase: is used for decomposing pectin in fish feed, and is convenient for fish to absorb.
The composite microorganism comprises: at least two of lactobacillus, streptococcus, Lactobacillus acidophilus, Lactobacillus bulgaricus, lactobacillus and bifidobacterium.
The compound microorganism can produce peptide bacteriocin, enzymes and a small amount of inorganic substances, and can inhibit the growth of harmful bacteria or prevent the adhesion and invasion of harmful bacteria toxin on epithelial cells. The nisin produced by the lactic acid bacteria and the streptococcus can inhibit the growth of salmonella, shigella, pseudomonas aeruginosa and escherichia coli; lactobacillus acidophilus and Lactobacillus bulgaricus can produce a small amount of hydrogen peroxide to inhibit the growth of gram-negative pathogenic bacteria; extracellular glycosidases produced by lactobacilli and bifidobacteria prevent the invasion of bacterial toxins.
Cattle, sheep, horse, pig, chicken, duck and goose manure, kitchen waste, fruit and vegetable waste, kitchen waste and sludge are subjected to material decomposition by adopting an anaerobic digestion process, and solid-liquid separation is carried out after the anaerobic digestion is finished to obtain biogas slurry.
For example: mixing pig, cattle, sheep, chicken and duck manure for anaerobic digestion: wherein the pig manure accounts for 60 percent, the cattle and sheep manure accounts for 20 percent, and the chicken and duck manure accounts for 20 percent; the nutrient content of the obtained biogas slurry is 40%.
Pig manure, kitchen waste and fruit and vegetable waste are subjected to mixed anaerobic digestion: wherein the pig manure accounts for 60%, the fruit and vegetable waste accounts for 30%, and the kitchen waste accounts for 10%; the nutrient content of the obtained biogas slurry is 55%.
Mixing the kitchen waste with sludge and kitchen waste for anaerobic digestion: wherein the sludge accounts for 40 percent, the kitchen waste accounts for 40 percent, and the kitchen waste accounts for 30 percent; the nutrient content in the biogas slurry is 43%.
Pig manure: the feed mainly comprises grains, and is added with a green vegetable feed, and the excrement is rich in saccharide and humic acid components.
Cow, sheep, horse manure: the feed is mainly green feed, and comprises dozens of green grasses and dry grasses, wherein the feed comprises part of Chinese herbal medicines, such as alfalfa, allium mongolicum regel and the like, and the feed of horses contains a small amount of grains.
Feces of chicken, duck and goose: the feed mainly comprises green vegetables, grains, fish, shrimp and insects, and a small amount of cobblestone, shell powder and the like.
Kitchen waste: the food waste eliminated in the catering industry, various meats, eggs, bones, vegetables, grains, fruits and the like.
Fruit and vegetable garbage: unqualified and rotten fruits and vegetables eliminated in agricultural production and urban markets are various in types and carry microorganisms.
Kitchen garbage: leftovers, fruit peels, fruit stones, fresh vegetables, fruits, meat and meat wastes, residual tea and the like eliminated from a kitchen in family life carry microorganisms.
Sludge: from domestic sewage treatment plants, mainly high-concentration polymers of domestic sewage pollutants, with microorganisms.
The composite microorganism used for anaerobic digestion is mainly methane flora, generally methane flora and acetic acid flora, and the content of the methane flora exceeds 18 percent and can reach 36 percent at most; the content of the acetic acid flora exceeds 20 percent and can reach 30 percent at most; other microorganisms mainly decompose and degrade organic biomass, mainly comprise various floras such as bacillus, rhodobacter, black carbon bacteria, escherichia coli and the like, and mainly have the functions of generating methane gas and decomposing the organic biomass.
The invention also provides a fish feed which is prepared by adopting the method for preparing the biological medicine fish feed by the biogas slurry and the hydrolyzed organic matters and is used for eliminating the fish leukoderma.
In the disease and epidemic elimination test by using the water-based microbial feed, the time for eliminating the leukoderma of the yellow croaker is only 3 days, the yellow croaker not only eliminates diseases, but also has bright and beautiful surface and obvious body weight gain, and the nutritional ingredients of the fish are far higher than those of the common culture through meat quality test, so that the yellow croaker is an organic food.
In the disease elimination test carried out by using the granulated fish feed or the dried granulated fish feed, the test result shows that the cure effect on the white spot is obvious, the occurrence and the diffusion of the aquaculture plague are eliminated and stopped, and the cure period is only three days; in the process of treating leukoderma, the feeding of the fish is not affected, and the fish can grow normally, quickly and healthily; the utilization rate of the feed exceeds 50 percent and is far higher than that of the common feed.
By adopting the scheme, the invention provides the method for preparing the biological medicine fish feed by using the biogas slurry and the hydrolyzed organic matters and the fish feed, and the water-based microorganism feed and the granular fish feed with the biological medicine function are produced by using the biogas slurry and the organic biomass as raw materials through the combination of various process methods and the refined selection of the raw materials, so that the disease prevention and control of aquaculture are realized. The organic biomass is thoroughly eliminated by high temperature and high pressure, the cleaning of the organic biomass is realized, so that the organic biomass can produce qualified, healthy and rich-nutrition fish feed, the fish feed has the function of producing organic aquatic food, and the high-value utilization of organic biomass waste is thoroughly realized.
Drawings
FIG. 1 is a schematic flow chart of the present invention.
Detailed Description
The invention is described in detail below with reference to the figures and the specific embodiments.
Referring to fig. 1, the present invention provides a method for preparing a biomedical fish feed from biogas slurry and hydrolyzed organic matter, comprising the following steps.
S1: adding complex enzyme and complex microorganism into anaerobically digested biogas slurry, and culturing for 24-120 h; obtaining the water-based microbial feed.
S2: and (3) the organic biomass is sent into a decomposition tank for decomposition, and after the decomposition is finished, the organic biomass is treated for 0.5 to 10 hours under the conditions of 1.2MPa and 200 ℃, and then dried to obtain the dried organic biomass with the water content of less than 20 percent. The high temperature and high pressure can eliminate all ova, mixed bacteria and various harmful residues in the organic biomass, and high-quality dry organic biomass is produced.
S3: drying the organic biomass and the aqueous microbial feed according to a ratio of 1: 0.5-3 weight ratio, and mixing and stirring to obtain pasty feed.
S4: and drying the pasty feed at normal temperature, and granulating when the water content of the pasty feed is 37-45% to obtain the granular fish feed.
S5: continuously drying the granulated fish feed at normal temperature to ensure that the water content of the granulated fish feed is lower than 35 percent, thereby obtaining the dried granulated fish feed; the density of the dried granulated fish feed is 0.6-0.8g/cm3, and the density after soaking in water is 0.93-0.99g/cm 3. The density of the granulated fish feed after soaking in water is less than that of water, so the granulated fish feed can float on the water surface and is convenient for fish to eat.
The compound enzyme comprises: protease, amylase, pectinase, cellulase, xylanase and beta-glucanase.
The complex enzyme can effectively decompose anti-nutritional factors in the fish feed, release nutrient substances, enable the nutrient substances to be fully digested and absorbed, improve the utilization rate of the fish feed and improve the production performance of animals. The xylanase effectively degrades the arabinoxylan in the fish feed, eliminates the anti-nutritional effect of the arabinoxylan on animals, and improves the utilization rate of fish feed resources. Beta-glucanase: eliminates the harm of beta-glucan in the fish feed, degrades the beta-glucan into absorbable reducing sugar and improves the nutritional value. Cellulase: cellulose in the fish feed is hydrolyzed into glucose, so that the utilization rate of the fish feed is greatly improved. Protease: the protein in the fish feed is hydrolyzed, which is beneficial to the absorption of the fish. Amylase: the starch in the fish feed is decomposed into glucose, which is beneficial to the absorption of the fish. And (3) pectinase: is used for decomposing pectin in fish feed, and is convenient for fish to absorb.
The composite microorganism comprises: lactic acid bacteria, streptococcus, Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus, and Bifidobacterium.
The compound microorganism can produce peptide bacteriocin, enzymes and a small amount of inorganic substances, and can inhibit the growth of harmful bacteria or prevent the adhesion and invasion of harmful bacteria toxin on epithelial cells. The nisin produced by the lactic acid bacteria and the streptococcus can inhibit the growth of salmonella, shigella, pseudomonas aeruginosa and escherichia coli; lactobacillus acidophilus and Lactobacillus bulgaricus can produce a small amount of hydrogen peroxide to inhibit the growth of gram-negative pathogenic bacteria; extracellular glycosidases produced by lactobacilli and bifidobacteria prevent the invasion of bacterial toxins.
Cattle, sheep, horse, pig, chicken, duck and goose manure, kitchen waste, fruit and vegetable waste, kitchen waste and sludge are subjected to material decomposition by adopting an anaerobic digestion process, and solid-liquid separation is carried out after the anaerobic digestion is finished to obtain biogas slurry.
In this embodiment, pig manure mixes anaerobic digestion with kitchen garbage, fruit vegetables rubbish: wherein the pig manure accounts for 60%, the fruit and vegetable waste accounts for 30%, and the kitchen waste accounts for 10%; the nutrient content of the obtained biogas slurry is 55%.
Pig manure: the feed mainly comprises grains, and is added with a green vegetable feed, and the excrement is rich in saccharide and humic acid components.
Kitchen waste: the food waste eliminated in the catering industry, various meats, eggs, bones, vegetables, grains, fruits and the like.
Fruit and vegetable garbage: unqualified and rotten fruits and vegetables eliminated in agricultural production and urban markets are various in types and carry microorganisms.
The composite microorganism used for anaerobic digestion is mainly methane flora, generally methane flora and acetic acid flora, and the content of the methane flora exceeds 18 percent and can reach 36 percent at most; the content of the acetic acid flora exceeds 20 percent and can reach 30 percent at most; other microorganisms mainly decompose and degrade organic biomass, mainly comprise various floras such as bacillus, rhodobacter, black carbon bacteria, escherichia coli and the like, and mainly have the functions of generating methane gas and decomposing the organic biomass.
The invention also provides a fish feed which is prepared by adopting the method for preparing the biological medicine fish feed by the biogas slurry and the hydrolyzed organic matters and is used for eliminating the fish leukoderma.
In the disease and epidemic elimination test by using the water-based microbial feed, the time for eliminating the leukoderma of the yellow croaker is only 3 days, the yellow croaker not only eliminates diseases, but also has bright and beautiful surface and obvious body weight gain, and the nutritional ingredients of the fish are far higher than those of the common culture through meat quality test, so that the yellow croaker is an organic food.
In the disease elimination test carried out by using the granulated fish feed or the dried granulated fish feed, the test result shows that the cure effect on the white spot is obvious, the occurrence and the diffusion of the aquaculture plague are eliminated and stopped, and the cure period is only three days; in the process of treating leukoderma, the feeding of the fish is not affected, and the fish can grow normally, quickly and healthily; the utilization rate of the feed exceeds 50 percent and is far higher than that of the common feed.
In conclusion, the invention provides a method for preparing a biological medicine fish feed by using biogas slurry and hydrolyzed organic matters and the fish feed, which realize the production of the water-based microorganism feed and the particle fish feed with the biological medicine function by using the biogas slurry and organic biomass as raw materials through the combination of various process methods and the refined selection of the raw materials, and solve the problem of disease control in aquaculture. The organic biomass is thoroughly eliminated by high temperature and high pressure, the cleaning of the organic biomass is realized, so that the organic biomass can produce qualified, healthy and rich-nutrition fish feed, the fish feed has the function of producing organic aquatic food, and the high-value utilization of organic biomass waste is thoroughly realized.
The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (6)
1. A method for preparing biological medicine fish feed by biogas slurry and hydrolyzed organic matters is characterized by comprising the following steps:
s1: adding complex enzyme and complex microorganism into anaerobically digested biogas slurry, and culturing for 24-120 h; obtaining an aqueous microbial feed;
s2: the organic biomass is sent into a decomposition tank for decomposition, after the decomposition is finished, the organic biomass is treated for 0.5 to 10 hours under the conditions of 1.2MPa and 200 ℃, and then dried to obtain dried organic biomass with the water content of less than 20 percent;
s3: drying the organic biomass and the aqueous microbial feed according to a ratio of 1: 0.5-3 weight ratio, and mixing and stirring into pasty feed;
s4: and drying the pasty feed at normal temperature, and granulating when the water content of the pasty feed is 37-45% to obtain the granular fish feed.
2. The method for preparing the biomedical fish feed from biogas slurry and hydrolyzed organic matter according to claim 1, further comprising the step of S5: continuously drying the granulated fish feed at normal temperature to ensure that the water content of the granulated fish feed is lower than 35 percent, thereby obtaining the dried granulated fish feed; the density of the dried fish feed granules is 0.6-0.8g/cm3The density after soaking in water is 0.93-0.99g/cm3。
3. The method for preparing the biomedical fish feed from biogas slurry and hydrolyzed organic matter according to claim 1, wherein the complex enzyme comprises: at least two of protease, amylase, pectinase, cellulase, xylanase and beta-glucanase.
4. The method for preparing the biomedical fish feed from biogas slurry and hydrolyzed organic matter according to claim 1, wherein the compound microorganisms comprise: at least two of lactobacillus, streptococcus, Lactobacillus acidophilus, Lactobacillus bulgaricus, lactobacillus and bifidobacterium.
5. The method for preparing the biomedical fish feed by biogas slurry and hydrolyzed organic matters according to claim 1, wherein the manure of cattle, sheep, horses, pigs, chickens, ducks and geese, kitchen waste, fruit and vegetable waste, kitchen waste and sludge are subjected to material decomposition by an anaerobic digestion process, and after the anaerobic digestion is completed, solid-liquid separation is performed to obtain biogas slurry.
6. A fish feed, which is characterized in that the biogas slurry and hydrolyzed organic matter of any one of claims 1 to 5 are used for preparing biological medicine fish feed for eliminating fish leukoderma.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111396852.3A CN114009632A (en) | 2021-11-23 | 2021-11-23 | Method for preparing biological medicine fish feed from biogas slurry and hydrolyzed organic matter and fish feed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111396852.3A CN114009632A (en) | 2021-11-23 | 2021-11-23 | Method for preparing biological medicine fish feed from biogas slurry and hydrolyzed organic matter and fish feed |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114009632A true CN114009632A (en) | 2022-02-08 |
Family
ID=80065945
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111396852.3A Pending CN114009632A (en) | 2021-11-23 | 2021-11-23 | Method for preparing biological medicine fish feed from biogas slurry and hydrolyzed organic matter and fish feed |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114009632A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104982759A (en) * | 2015-08-07 | 2015-10-21 | 灵川县億旺养殖专业合作社 | Bagasse fermentation hog feed and preparing method thereof |
| CN105010879A (en) * | 2015-08-07 | 2015-11-04 | 灵川县億旺养殖专业合作社 | Cassava residue fermentation pork pig feed and preparation method thereof |
| CN105918702A (en) * | 2016-04-29 | 2016-09-07 | 成都绿金生物科技有限责任公司 | Neem oil feed for fish and preparation method thereof |
| CN107129324A (en) * | 2017-06-22 | 2017-09-05 | 浏阳大宗农业有限责任公司 | A kind of Large-scale pig farm fecaluria resource utilization method |
| CN109965151A (en) * | 2019-04-22 | 2019-07-05 | 刘坚 | A kind of fish meal and preparation method thereof in pig manure source |
| CN110169506A (en) * | 2019-06-12 | 2019-08-27 | 犍为蜀姜生物科技有限公司 | A kind of turmeric dreg fermentation pannage and preparation method thereof |
-
2021
- 2021-11-23 CN CN202111396852.3A patent/CN114009632A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104982759A (en) * | 2015-08-07 | 2015-10-21 | 灵川县億旺养殖专业合作社 | Bagasse fermentation hog feed and preparing method thereof |
| CN105010879A (en) * | 2015-08-07 | 2015-11-04 | 灵川县億旺养殖专业合作社 | Cassava residue fermentation pork pig feed and preparation method thereof |
| CN105918702A (en) * | 2016-04-29 | 2016-09-07 | 成都绿金生物科技有限责任公司 | Neem oil feed for fish and preparation method thereof |
| CN107129324A (en) * | 2017-06-22 | 2017-09-05 | 浏阳大宗农业有限责任公司 | A kind of Large-scale pig farm fecaluria resource utilization method |
| CN109965151A (en) * | 2019-04-22 | 2019-07-05 | 刘坚 | A kind of fish meal and preparation method thereof in pig manure source |
| CN110169506A (en) * | 2019-06-12 | 2019-08-27 | 犍为蜀姜生物科技有限公司 | A kind of turmeric dreg fermentation pannage and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 张玲等: "香蕉茎叶生物活性鱼用饲料的发酵工艺优化", 广东石油化工学院学报, vol. 28, no. 1, pages 12 - 17 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106173363B (en) | The method for producing cattle and sheep complete feed as raw material staged fermentation using edible fungus bran | |
| CN101449744B (en) | Multifunctional biology aquatic feedstuff additive | |
| CN103859155B (en) | Containing the process for purification of profitable probliotics cassava feed | |
| CN103664287B (en) | A kind of biological organic fertilizer utilizing waterplant to prepare | |
| CN101390566A (en) | Manifold microbe mixed culture fermentation agent and method for producing high energy protein biology feedstuff | |
| CN103073113A (en) | Microorganism restoration method for water body habitat | |
| CN102286376A (en) | Microbial inoculum for high-efficiency fermenting bed and preparation method thereof | |
| CN103829042A (en) | Production method of multi-vitamin active cassava protein feed | |
| CN106615658A (en) | Method for preparing fermentation bed padding by recycling froggrass | |
| CN103027188A (en) | Deep processing method of pineapple skin and residues | |
| CN112741234A (en) | Efficient breeding feed for hermetia illucens and breeding method thereof | |
| CN106306426A (en) | Novel process for corn silage | |
| CN111990529A (en) | Microbial fermentation method for preparing insect soluble liquid by utilizing hermetia illucens larvae and application | |
| CN102154145A (en) | Microorganism environment modifier and microorganism packing material | |
| CN1548405A (en) | Method for producing efficient active biological organic fertilizer with excrement and organic garbage | |
| CN107466877A (en) | A kind of ox bed biological pad and its application method | |
| CN106146200A (en) | A kind of method utilizing ight soil and organic waste production biological organic fertilizer | |
| CN112998130B (en) | Fruit residue and crop straw mixed fermentation feed and preparation method thereof | |
| CN105084551A (en) | Aquaculture ecological environment remediation method | |
| CN110078539A (en) | A kind of harmless biological degradation agent and preparation method thereof | |
| KR100191259B1 (en) | Method for preparing feed using food wastes | |
| CN113414226A (en) | Kitchen waste leavening agent and preparation method and application thereof | |
| CN105218253A (en) | A kind of technique utilizing animal intestine gastric content and ight soil to produce fertilizer | |
| CN109777757B (en) | Composite fermentation bed microbial inoculum and preparation method and application thereof | |
| CN116874316A (en) | Method for high-value conversion of agricultural waste by cooperation of hermetia illucens and microorganisms |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220208 |