CN113994790B - Method for promoting quick germination and seedling emergence of paris polyphylla rhizome buds - Google Patents
Method for promoting quick germination and seedling emergence of paris polyphylla rhizome buds Download PDFInfo
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Abstract
The invention relates to a method for promoting paris polyphylla rhizome buds to quickly germinate and emerge, and belongs to the technical field of dormancy breaking of dormant buds of plants. The method comprises the following steps: 1) carrying out low-temperature treatment on paris polyphylla rhizome buds at 0-15 ℃ for 10-40 days to obtain low-temperature treated dormant buds; 2) placing the low-temperature treated dormant buds obtained in the step 1) in 50-200 mg.L‑1Soaking the gibberellin in the gibberellin aqueous solution for 4-10 hours to obtain dormant buds treated by gibberellin; 3) sand-storing the gibberellin-treated dormant buds obtained in the step 2), and applying 2-20 mg.L once every 10 days‑1The diethyl aminoethyl hexanoate aqueous solution realizes the rapid germination of the paris polyphylla rhizome buds. The method disclosed by the invention is simple to operate, has a stable effect, and can achieve the purpose of rapidly inducing the dormant rhizome buds of the paris polyphylla to germinate and emerge.
Description
Technical Field
The invention relates to the technical field of dormancy breaking of dormant buds of plants, in particular to a method for promoting quick germination and seedling emergence of paris polyphylla rhizome buds.
Background
Parisphylla (Parisphylla Smith var. Chinensis (Franch.) Hara.) is a perennial herb of Paris of Liliaceae, also called Paris polyphylla Smith, Paris polyphylla Lamiophlomis, and the like, is recorded by the name of Paris polyphylla in Shennong herbal longitude as the earliest and is one of the sources of two basic crude drugs, namely Paris polyphylla, recorded in the 2020 edition of Chinese pharmacopoeia. Rhizoma paridis takes dry rhizome as medicine and has the efficacies of clearing heat and detoxicating, relieving swelling and pain, cooling liver and arresting convulsion, and the like. Modern pharmacological studies show that the rhizoma paridis also has the effects of resisting cancer, stopping bleeding, eliminating phlegm, inhibiting bacteria, relieving cough and asthma, tranquilizing, relieving pain, resisting early pregnancy, killing sperms, resisting cytotoxicity and the like. In recent years, research shows that the Paris polyphylla has an inhibiting effect on Asian influenza A virus. The novel coronavirus SARS-CoV-2 is mainly combined with Angiotensin-converting enzyme 2(ACE2) receptor on the surface of human cell by S protein, and the molecular docking method is utilized to simulate and predict the combination affinity of 3 paridis saponins (I, VI, VII) rich in Paris polyphylla and ACE2, and the result shows that the structural design based on the three effective components is expected to obtain the efficient SARS-CoV-2 inhibitor. With the deepening of the research of the Paris polyphylla, the application range is continuously expanded, the market demand is greatly increased, the consumption is far higher than the growth amount of wild resources, and the phenomenon of excessive mining and disorderly digging also occurs in a main production area, so that the wild resources are gradually exhausted.
In the process of artificially cultivating the Paris polyphylla, the dormancy phenomenon of the Paris polyphylla rhizome buds is found to be the same as the seeds. After the overground part of the rhizoma paridis is poured into the seedlings in autumn and winter, the buds need to pass through a growth lag period of a period of time, can not induce the germination even if proper environmental conditions are given in the growth lag period, and can germinate and emerge only after accumulating certain low temperature in winter to the beginning of spring of the second year. Combining with the classification standard of Lang et al (1987) on bud dormancy, the growth of the Paris polyphylla conforms to the division of the annual period of perennial plants by Champagnat (1989), the rhizome buds need to go through an internal dormancy period of a period of time after the seedlings of the Paris polyphylla are poured in autumn and winter, and the dormancy period is usually 3-4 months. When the rhizome is used for cutting into blocks for propagation, the rhizome buds cannot start to grow in time due to the existence of a dormancy mechanism, and are rotten and apoptotic; meanwhile, the dormancy of the paris polyphylla buds increases the cost of rhizome preservation and management and protection and also prolongs the period of the drug formation of the paris polyphylla. In conclusion, the bud dormancy may become the limitation for the development of the Paris polyphylla industry in the new trend along with the application of the greenhouse technology to the artificial cultivation of the Paris polyphylla. At present, no study on dormancy breaking of paris polyphylla rhizome buds exists.
Disclosure of Invention
The invention aims to provide a method for promoting paris polyphylla rhizome buds to quickly germinate and emerge. The method disclosed by the invention is simple to operate, has a stable effect, and can achieve the purpose of rapidly inducing the dormant rhizome buds of the paris polyphylla to germinate and sprout.
The invention provides a method for promoting paris polyphylla rhizome buds to quickly germinate and emerge, which comprises the following steps:
1) carrying out low-temperature treatment on the paris polyphylla rhizome buds at the temperature of 0-15 ℃ for 10-40 days to obtain low-temperature treated dormant buds;
2) putting the low-temperature processed dormant buds obtained in the step 1) in 50 to200mg·L-1Soaking the gibberellin in the gibberellin aqueous solution for 4-10 hours to obtain dormant buds treated by gibberellin;
3) sand-storing the dormant buds treated by the gibberellin obtained in the step 2), and applying 2-20 mg.L to the dormant buds every 10 days-1The diethyl aminoethyl hexanoate aqueous solution realizes the rapid germination of the paris polyphylla rhizome buds.
Preferably, before the low-temperature treatment in the step 1), the method further comprises the operations of cleaning, sterilizing and packaging the paris polyphylla rhizome buds; the disinfection comprises soaking with carbendazim; the packaging includes packaging with a perforated film.
Preferably, in the step 1), the low-temperature treatment is carried out at 5-10 ℃ for 20-30 d.
Preferably, in the step 2), the mass concentration of the gibberellin in the gibberellin water solution is 100-150 mg-L-1And the soaking time is 6-8 h.
Preferably, in the step 2), the temperature of the soaking is 20 ℃.
Preferably, the sand storage in the step 3) keeps 1.0cm of the exposed sand surface of the buds of the paris polyphylla rhizome buds.
Preferably, the sand in step 3) is stored, the sand is kept moist, the air is circulated, and direct sunlight is avoided.
Preferably, in the step 3), the mass concentration of the diethyl aminoethyl hexanoate in the diethyl aminoethyl hexanoate aqueous solution is 5-10 mg/L-1。
Preferably, the sand storage temperature in the step 3) is 18-22 ℃.
Preferably, the sand storage in the step 3) keeps illumination intensity at 3800lx and light cycle at 7-12 h.d-1。
The invention provides a method for promoting paris polyphylla rhizome buds to quickly germinate and emerge. The method takes the rhizoma paridis rhizome buds which naturally fall into dormancy after seedling falling as the material, the rhizoma paridis rhizome buds are placed in a sand storage in a suitable environment after being treated by low temperature and gibberellin, and the aim of rapidly inducing the rhizoma paridis rhizome buds to sprout can be achieved by periodically spraying diethyl aminoethyl hexanoate. After seedling emergence, the greenhouse technology is combined, so that the growth time of the overground part of the Paris polyphylla can be prolonged, the accumulation of effective ingredients and biomass of rhizomes is accelerated, the medicine forming time of the Paris polyphylla is shortened, and the method has important significance for the development of the Paris polyphylla industry. The method is simple to operate, has the characteristics of quick bud emergence, stable induction effect and the like, and can provide technical reference for realizing the non-dormancy cultivation of the new rhizoma paridis facility cultivation mode. Test results show that the method of the invention has the advantages that the time of emergence of the buds is 10-50 days earlier than that of the buds of untreated rhizome, the buds and the rhizome grow well after emergence of the seedlings, the life cycle can be completed normally, the dormancy breaking effect of the invention is stable, and the emergence rate is high.
Detailed Description
The invention provides a method for promoting paris polyphylla rhizome buds to quickly germinate and emerge, which comprises the following steps:
1) carrying out low-temperature treatment on paris polyphylla rhizome buds at 0-15 ℃ for 10-40 days to obtain low-temperature treated dormant buds;
2) placing the low-temperature treated dormant buds obtained in the step 1) in a range of 50-200 mg.L-1Soaking the gibberellin in the gibberellin aqueous solution for 4-10 hours to obtain dormant buds treated by gibberellin;
3) sand-storing the dormant buds treated by the gibberellin obtained in the step 2), and applying 2-20 mg.L to the dormant buds every 10 days-1The diethyl aminoethyl hexanoate aqueous solution realizes the rapid germination of the paris polyphylla rhizome buds.
The method comprises the step of carrying out low-temperature treatment on rhizoma paridis rhizome buds at 0-15 ℃ for 10-40 days to obtain low-temperature treated dormant buds. The invention preferably selects healthy and disease-free paris polyphylla rhizome buds. In the invention, before the low-temperature treatment, the method preferably further comprises the operations of cleaning, disinfecting and packaging the paris polyphylla rhizome buds; the disinfection comprises soaking with carbendazim; the packaging includes packaging with a perforated film. The method of the present invention for cleaning is not particularly limited, and a conventional cleaning method known to those skilled in the art may be used. In the invention, the disinfection is preferably carried out by soaking with 0.1% of carbendazim by mass, and the soaking time is preferably 30-60 min, and more preferably 30 min. After sterilization, the present invention is packaged, preferably using plastic film, which is preferably subjected toAnd perforating for ventilation. In the present invention, the perforated density of the film is preferably 12/m2. After packaging, the present invention preferably uses wet fine sand which is sterilized in advance as a filler, and the packaged rhizome buds are subjected to constant temperature treatment under low temperature condition, namely low temperature treatment. In the invention, the temperature of the low-temperature treatment is more preferably 5-10 ℃, most preferably 5 ℃, and the time is more preferably 20-30 d, most preferably 25 d. The invention preferably controls the temperature precision to be +/-0.5 ℃. The low-temperature treatment condition is set, so that the minimum cold requirement for dormancy breaking of the rhizoma paridis rhizome buds can be met. At present, no method for releasing dormancy of paris polyphylla rhizome buds and promoting early seedling emergence of dormant buds exists, and the lowest cold requirement required by promoting bud seedling emergence of the invention can be quickly achieved by the low-temperature treatment method of the invention.
After the dormant bud is obtained, the dormant bud is placed in 50-200 mg.L-1Soaking the rice in the gibberellin aqueous solution for 4-10 hours to obtain dormant buds treated by gibberellin. In the present invention, after the dormant buds treated at low temperature are taken out, it is preferable to remove the necrotic or poorly growing rootstocks. In the invention, the mass concentration of gibberellin in gibberellin water solution is more preferably 100-150 mg.L-1Most preferably 100 mg.L-1The soaking time is more preferably 6-8 h, and most preferably 8 h. In the present invention, the temperature of the soaking is preferably 20 ℃. The method can promote the germination and seedling emergence of the buds by treating the dormant rhizome buds of the paris polyphylla at low temperature with gibberellin under proper conditions.
After obtaining the gibberellin-treated dormant buds, the gibberellin-treated dormant buds are subjected to sand storage, and 2-20 mg.L of gibberellin-treated dormant buds are applied every 10 days-1The diethyl aminoethyl hexanoate aqueous solution realizes the rapid germination of rhizoma paridis rhizome buds. The present invention preferably transfers the gibberellin-treated dormant buds to a nursery room for sand storage. In the present invention, the sand storage preferably maintains 1.0cm of the sand surface exposed from the buds of the rhizoma paridis rhizome buds. In the present invention, the sand reservoir preferably keeps the sand moist and air-permeable, avoiding direct sunlight. The invention preferably waters periodically to keep sand moist, more preferably to keep the humidity of sand at 3040 percent (grasping the sand by hand to form a ball, and loosening the sand to disperse the sand). The manner in which the diethyl aminoethyl hexanoate is applied according to the invention is preferably by drenching. In the invention, the mass concentration of the diethyl aminoethyl hexanoate in the diethyl aminoethyl hexanoate aqueous solution is more preferably 5-10 mg.L-1Most preferably 8 mg.L-1. In the invention, the sand storage temperature is preferably 18-22 ℃, and more preferably 20 ℃. In the invention, the sand storage preferably keeps illumination intensity at 3800lx and the photoperiod at 7-12 h.d-1More preferably 8 h.d-1. After the cultivation for a period of time, the germination and emergence of seedlings can be observed, and the emergence time is 10-50 days earlier than that of untreated rhizome buds. The invention stores the buds treated by low temperature and gibberellin in sand under the specific conditions, and regularly drenches diethyl aminoethyl hexanoate with proper concentration, thereby playing the role of quickly inducing the emergence of the buds. The method comprises the steps of placing the rootstock buds in a seedling growing chamber for sand storage treatment, avoiding damage caused by frost, slightly exposing the buds to a sand surface during sand storage, enabling the buds to better feel illumination, then treating the buds with diethyl aminoethyl hexanoate with proper concentration, accelerating the accumulation of photochromism in the buds, improving the activities of peroxidase and nitrate reductase, promoting the carbon and nitrogen metabolism of plants, enhancing the absorption of the plants on water and fertilizer and the accumulation of dry substances, adjusting the proportion of free water and bound water in vivo, promoting the release process of bud dormancy, and finally inducing the buds to sprout in advance. By using the sand storage and treatment method, the seedling emergence time can be advanced by 10-50 d, the storage time is shortened, and the photosynthesis period is prolonged.
The method for breaking the buds of the paris polyphylla to sleep is not reported so far. The rhizoma paridis used in the invention is usually transplanted to seedlings at the beginning of 12 months, seedlings emerge from the end of the next two months to the beginning of the third month, and the dormancy period is about 90-100 days. In the invention, the rhizome buds of the paris polyphylla treated by low temperature, gibberellin, sand storage and diethyl aminoethyl hexanoate have the emergence time 10-50 days earlier than that of untreated buds, and the emergence of seedlings can be observed at the bottom of a month at the fastest speed. The untreated bud refers to dormant bud which is naturally poured and transplanted into a seedling room at the temperature of 20 +/-2 ℃, is not treated by low temperature, gibberellin and diethyl aminoethyl hexanoate and is stored in sand according to a conventional management method, and is watered regularly to keep sand moist. The sprouts emerge in advance, the growth cycle of overground parts can be increased, and because the plants are shifted to a vigorous growth period before warm and humid weather comes, the plants have strong disease resistance, the crisis of dead seedlings caused by diseases can be reduced, and the method has important significance for the growth development and the industrialized development of the Paris polyphylla. The present paris polyphylla rhizome multiple-bud inducing technology has certain breakthrough, and the rhizome multiple-bud inducing technology is possible, but the induced lateral bud has the same dormancy phenomenon.
The following describes the method for promoting the rapid germination and emergence of paris polyphylla rhizome buds in detail with reference to specific embodiments, and the technical scheme of the invention includes but is not limited to the following embodiments.
Example 1
1) And (3) low-temperature treatment of dormant buds: selecting healthy and disease-free rhizoma paridis rhizome buds, cleaning, soaking in 0.1% carbendazim for 30min for sterilization, and packaging with plastic film (12 pieces/m)2And the holes are punched for ventilation. Wet fine sand which is sterilized in advance is used as a filler, and then the packaged rhizome buds are placed at the constant temperature of 5 ℃ for treatment for 25 days, wherein the temperature precision is +/-0.5;
2) gibberellin treatment of dormant buds: taking out the low-temperature treated rootstock bud, removing necrotic or poor-growth rootstock, and germinating at 100 mg.L-1Soaking in gibberellin water solution for 8 h;
3) inducing and germinating dormant buds: the rhizomes treated by gibberellin at low temperature are transferred to the temperature of 20 +/-2 ℃ and the photoperiod of 8h d-1The seedling growing room is sand-stored, the sprouts are exposed to 1.0cm of sand surface when the sprouts are sand-stored, the sprouts are watered periodically to keep the sand moist and keep the air circulation, and 8 mg.L of the sprout is sprayed every 10 days-1The diethyl aminoethyl hexanoate aqueous solution can induce the bud to emerge in 25 days; the method only needs 50 days from the beginning of low-temperature treatment to the observation of the fastest bud emergence, the bud emergence rate can reach more than 92%, and the seedlings grow robustly, the roots grow well and the roots are developed.
Example 2
Example 1 was repeated except that:
modifying the low-temperature treatment time at 5 ℃ in the step 1) into: 20 d; the seedlings can be induced to emerge after being stored in sand for 42 days, the time from low-temperature treatment to seedling emergence is 62 days, the seedling emergence rate is 85 percent, the seedlings grow robustly after emergence, the roots grow well, and the roots are developed; probably because the low-temperature treatment time is short and the cold requirement does not reach the standard of bud emergence, the sand storage time is required to be longer for accumulation.
Example 3
Example 1 was repeated except that:
modifying the concentration of gibberellin in the step 2) as follows: 50 mg. L-1(ii) a After sand storage for 33 days, seedling emergence can be induced, the time from low-temperature treatment to seedling emergence is 58 days, which is 8 days longer than that of example 1, the seedling emergence rate is 88%, the seedling grows strongly, the rhizome grows well, and the root system is developed; gibberellin can partially replace low temperature, promote the release of internal dormancy, reduce the concentration of gibberellin, possibly cause insufficient low-temperature compensation amount, and thus increase the seedling emergence time.
Example 4
Example 2 was repeated except that:
the concentration of diethyl aminoethyl hexanoate in the step 3) is modified to be 10 mg.L-1(ii) a Seedlings can be induced to emerge after sand storage for 45 days, the time from low-temperature treatment to seedling emergence is 65 days, the seedling emergence rate of the seedlings is 86 percent, the seedlings grow strongly after emergence, rootstocks grow well, and root systems are developed; the high concentration of diethyl aminoethyl hexanoate may inhibit the viability of the shoots, resulting in a decrease in the rate of emergence of the shoots; meanwhile, the problem of insufficient cooling capacity caused by shortened low-temperature treatment time cannot be solved by the high-concentration diethyl aminoethyl hexanoate, so that the seedling emergence time is prolonged.
Example 5
Example 2 was repeated except that:
the photoperiod time in the step 3) is changed into 12 h.d-1(ii) a Seedling emergence can be induced after sand storage for 43 days, the time from low-temperature treatment to seedling emergence is 63 days, the seedling emergence rate is 88 percent, the seedlings grow robustly, the roots grow well and the roots are developed; the sunshine duration increases, and the destruction of the bud dormancy may be rather slightly inhibited, resulting in a prolonged emergence time.
Example 6
Example 2 was repeated except that:
the gibberellin soaking time in the step 2) is changed into that: 10 h; 47d after sand storage can induce seedling emergence, the time from low-temperature treatment to seedling emergence is increased by 67d compared with 1 in example 1, the seedling emergence rate is 87%, the seedling after emergence grows robustly, the rootstocks grow well, and the root systems are developed; the gibberellin treatment time is increased, the GA/ABA ratio is possibly influenced, or long-time hormone soaking is not beneficial to growth of rhizome buds, so that the seedling emergence time is prolonged.
Example 7
Example 3 was repeated except that:
modifying the low-temperature treatment temperature in the step 1) into the following temperature: 10 ℃; after the seedlings are stored in sand for 37 days, seedling emergence can be induced, the time from low-temperature treatment to seedling emergence is 62 days, the seedling emergence rate is 90 percent, the seedlings grow robustly, the roots grow well, and the roots are developed; probably because the treatment temperature is increased and the treatment time is unchanged, the cold requirement does not reach the standard of bud emergence, and the emergence time is prolonged.
Example 8
Example 3 was repeated except that:
the photoperiod in the step 3) is modified to be 7 h.d-1The concentration of diethyl aminoethyl hexanoate was modified to 5 mg. L-1(ii) a The seedlings can be induced to emerge after being stored in sand for 41 days, the time from low-temperature treatment to seedling emergence is 66 days, the seedling emergence rate is 83 percent, the seedlings grow robustly after emergence, the roots grow well, and the roots are developed. After the illumination period is shortened, the seedling emergence time is 8d longer than that of the embodiment 3; the short sun can cause growth stop, probably results in the increase of abscisic acid (ABA) synthesis of plants under the short-day condition, the GA synthesis is reduced, and when the ABA/GA ratio is increased, the dormancy induction effect is realized, so the emergence time is increased.
Example 9
Example 3 was repeated except that:
modifying the low-temperature treatment time in the step 1) into: 30 d; after sand storage for 45 days, seedling emergence can be induced, the time from low-temperature treatment to seedling emergence is 70 days, and the seedling emergence rate is 75%; the emergence time was increased by 12d compared to example 3, and prolonged low temperatures may result in reduced bud growth activity, resulting in a reduction in emergence rate.
Comparative example 1
Example 1 was repeated except that:
modifying gibberellin in the step 2) into: indoleacetic acid IAA; emergence was observed at 62d after sand storage, and the time from low temperature treatment to emergence was about 87d, which is comparable to the time of emergence of untreated shoots. Probably, indoleacetic acid can not partially replace low temperature like gibberellin, so that the minimum cold requirement required by bud germination can not be met, dormancy can not be effectively broken, and the emergence time is close to the emergence time of untreated buds.
Comparative example 2
Example 1 was repeated except that:
modifying the concentration of diethyl aminoethyl hexanoate in the step 3) into: 50 mg. L-1(ii) a After sand storage, the buds grow poorly, the root systems fall off and die, the emergence rate is only 35%, and the stems and leaves after emergence are fine. The high-concentration diethyl aminoethyl hexanoate is possibly not beneficial to the growth of root systems, so that the root systems fall off and the activity is reduced, thereby influencing the absorption of nutrients, causing poor bud growth, poor development of stem leaves after emergence of seedlings and fine stem leaves.
Example 10
1) And (3) low-temperature treatment of dormant buds: selecting healthy and disease-free rhizoma paridis rhizome buds, cleaning, soaking in 0.1% carbendazim for 60min for sterilization, and packaging with plastic film (12/m)2And perforating for ventilation. Using sterilized wet fine sand as filler, and then placing the packaged rhizome buds at 0 ℃ for constant temperature treatment for 10 days with the temperature precision of +/-0.5;
2) gibberellin treatment of dormant buds: taking out the low-temperature treated rhizome bud, removing necrotic or poor bud growth rhizome, and sprouting the rhizome at 200 mg.L-1Soaking in gibberellin water solution for 4 h;
3) inducing and germinating dormant buds: the rhizomes of the gibberellin treated rhizomes are transferred to a temperature of 20 +/-2 ℃ and a photoperiod of 12 h.d-1The seedling raising room is stored in sand, buds are not exposed when the seedlings are stored in the sand, the seedlings are watered periodically to keep the sand moist and keep the air circulation, and 2 mg.L of the water is sprayed every 10 days-1The diethyl aminoethyl hexanoate aqueous solution can induce the bud to emerge after being stored in sand for 60 daysThe germination rate of the buds is 80 percent, the stem leaves grow well after emergence of the seedlings, and the root systems are slender. The time from low-temperature treatment to seedling emergence is about 70 days, which is 20-30 days earlier than that of an untreated group, and the fact that the dormant buds can be effectively induced to seedling emergence in advance in the range of each treatment in the invention is shown; in addition, the change of any treatment condition possibly influences the dormancy breaking of the dormant buds, and the treatment of the embodiment 1 has the advantages of best dormancy breaking effect, short emergence time, high emergence rate, robust growth after the emergence of the buds, good rhizome growth and developed root system.
Example 11
1) Low-temperature treatment of dormant buds: selecting healthy and disease-free rhizoma paridis rhizome buds, cleaning, soaking in 0.1% carbendazim for 30min for sterilization, and packaging with plastic film (12/m)2And perforating for ventilation. Using sterilized wet fine sand as filler, then placing the packaged rhizome buds at 15 ℃ for constant temperature treatment for 30 days, wherein the temperature precision is +/-0.5;
2) gibberellin treatment of dormant buds: taking out the low-temperature treated rootstock bud, removing necrotic or poor-growth rootstock, and germinating at 100 mg.L-1Soaking in gibberellin water solution for 6 h;
3) inducing and germinating dormant buds: the rhizomes of the gibberellin-treated rhizomes are transferred to a temperature of 20 +/-2 ℃ and a photoperiod of 7 h.d-1The seedling raising room is sand-stored, when sand-stored, the bud is exposed to 1.0cm of sand surface, periodically watered to keep sand moist and keep air circulation, and 8 mg.L is sprayed every 10 days-1The diethyl aminoethyl hexanoate aqueous solution can induce the germination of the buds after being stored in sand for 50 days, the germination rate of the buds is more than 87%, and the buds grow well after emergence of the seedlings. The time from low-temperature treatment to seedling emergence is about 80 days, and the low-temperature treatment temperature is probably higher, so that the treatment time and gibberellin soaking are increased, but a longer sand storage time is still needed to supplement the lowest cold demand required by seedling emergence. The emergence time is 10-20 days earlier than that of an untreated group, which shows that the embodiment can still effectively induce dormant buds to emerge in advance.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and amendments can be made without departing from the principle of the present invention, and these modifications and amendments should also be considered as the protection scope of the present invention.
Claims (3)
1. A method for promoting the rapid germination and emergence of paris polyphylla rhizome buds comprises the following steps:
1) carrying out low-temperature treatment on the paris polyphylla rhizome buds at the temperature of 5-10 ℃ for 20-25 days to obtain dormant buds subjected to low-temperature treatment; the paris polyphylla rhizome buds are dormant paris polyphylla rhizome buds after natural seedling pouring;
2) placing the low-temperature treated dormant buds obtained in the step 1) at 50-100 mg/L-1Soaking the gibberellin in the gibberellin aqueous solution for 6-10 hours to obtain dormant buds treated by gibberellin;
3) sand-storing the gibberellin-treated dormant buds obtained in the step 2), and applying 5-10 mg.L every 10 days-1The diethyl aminoethyl hexanoate aqueous solution realizes the rapid germination of rhizoma paridis rhizome buds; the sand storage keeps the buds of the rhizoma paridis rhizome buds exposed to 1.0cm of the sand surface, sand is wet, air is circulated, and direct sunlight is avoided; the sand storage temperature is 18-22 ℃; the sand storage needs to keep illumination intensity at 3800lx and light period at 7-12 h.d-1。
2. The method as claimed in claim 1, wherein before the low-temperature treatment in step 1), the method further comprises the steps of cleaning, sterilizing and packaging rhizoma paridis rhizome buds; the disinfection comprises soaking with carbendazim; the packaging includes packaging with a perforated film.
3. The method according to claim 1, wherein the temperature of the soaking in the step 2) is 20 ℃.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101142881A (en) * | 2007-10-18 | 2008-03-19 | 云南省农业科学院高山经济植物研究所 | Roots and stems section-cutting seedling-growing method for paris yunnanensis franch |
| JP2011092072A (en) * | 2009-10-29 | 2011-05-12 | Hirosaki Univ | Bulb growth promoter for liliaceae plant, and method for promoting growth of bulb of liliaceae plant |
| CN103430838A (en) * | 2013-07-19 | 2013-12-11 | 四川同道堂药业集团股份有限公司 | New technology of inducing clustered shoots from parispolyphylla rhizome for rapid vegetative propagation |
| CN105123253A (en) * | 2015-10-15 | 2015-12-09 | 王婧 | Seedling method for paris polyphylla |
| CN105723998A (en) * | 2014-12-12 | 2016-07-06 | 丽江云全生物开发有限公司 | Polygerm paris yunnanensis franch. underground rhizome cutting propagation method |
| CN107182521A (en) * | 2017-06-26 | 2017-09-22 | 长沙慧日生物技术有限公司 | Magnificent Paris polyphylla high efficiency seedling cultivating method |
| CN108650926A (en) * | 2017-03-30 | 2018-10-16 | 浙江中医药大学 | Method of the exogeneous growth conditioning agent to abolishing magnificent Paris polyphylla seed dormancy |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105104213B (en) * | 2015-10-15 | 2017-05-03 | 湖南农业大学 | Rapid propagation and seedling culture method using rhizomes of paris polyphylla |
| CN107278579B (en) * | 2017-07-20 | 2020-04-07 | 四川省中医药科学院 | Fast-growing breeding method of Paris polyphylla rhizome seedlings without destroying original habitat |
-
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Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101142881A (en) * | 2007-10-18 | 2008-03-19 | 云南省农业科学院高山经济植物研究所 | Roots and stems section-cutting seedling-growing method for paris yunnanensis franch |
| JP2011092072A (en) * | 2009-10-29 | 2011-05-12 | Hirosaki Univ | Bulb growth promoter for liliaceae plant, and method for promoting growth of bulb of liliaceae plant |
| CN103430838A (en) * | 2013-07-19 | 2013-12-11 | 四川同道堂药业集团股份有限公司 | New technology of inducing clustered shoots from parispolyphylla rhizome for rapid vegetative propagation |
| CN105723998A (en) * | 2014-12-12 | 2016-07-06 | 丽江云全生物开发有限公司 | Polygerm paris yunnanensis franch. underground rhizome cutting propagation method |
| CN105123253A (en) * | 2015-10-15 | 2015-12-09 | 王婧 | Seedling method for paris polyphylla |
| CN108650926A (en) * | 2017-03-30 | 2018-10-16 | 浙江中医药大学 | Method of the exogeneous growth conditioning agent to abolishing magnificent Paris polyphylla seed dormancy |
| CN107182521A (en) * | 2017-06-26 | 2017-09-22 | 长沙慧日生物技术有限公司 | Magnificent Paris polyphylla high efficiency seedling cultivating method |
Non-Patent Citations (1)
| Title |
|---|
| 云南重楼种苗繁育技术;陈翠等;《中国现代中药》;20100216(第02期);第23-24，28页 * |
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