CN113860959A

CN113860959A - Preparation method of selenium-rich rice stem cell growth promoter

Info

Publication number: CN113860959A
Application number: CN202111142101.9A
Authority: CN
Inventors: 张家齐; 张良; 吴宝元; 李春雨
Original assignee: Chunrun Agricultural Biotechnology Panjin Co ltd
Current assignee: Chunrun Agricultural Biotechnology Panjin Co ltd
Priority date: 2021-09-28
Filing date: 2021-09-28
Publication date: 2021-12-31

Abstract

The invention discloses a method for preparing a selenium-rich rice stem cell growth promoter, relates to the technical field of plant nutrition, and aims to solve the problem that the root system of rice is slow in the growth process. After the promoter is applied, the growth is rapid, the stem and leaf are luxuriant, the root system is developed, the disease resistance is strong, and the nutrition of the fruits is rich.

Description

Preparation method of selenium-rich rice stem cell growth promoter

Technical Field

The invention relates to the technical field of plant nutrition, in particular to a preparation method of a selenium-rich rice stem cell growth promoter.

Background

The rice is one of the staple food grains in China, is used as a large-area agricultural product and is planted in a large area at home, but the content of nutrient components and trace elements in the rice is continuously reduced due to the loss of soil nutrition after long-term cultivation, so that the health of the public is greatly influenced.

Plant stem cells are relative to mature tissues that have differentiated to mature within the plant. Typically, a plant's stem cells are two "clumps" of cells within the plant. These two clusters of cells are located in the apical meristem and root apical meristem of the plant, respectively. Plant stem cells are the root cause of plant viability. The plant stem cells have remarkable regeneration capacity, and the deep-rooted and lobate plants can be continuously promoted only by promoting the growth of the plant stem cells.

In the process of fertilizing the rice, as the root system is immersed in water, the components of the fertilizer are easy to dilute, so that the cost is increased, in order to better supplement nutrients and promote the growth of rice stem cells, the selenium-rich fertilizer with the slow release capability is needed, and guarantee is provided for the safety of grains and the health of the public.

Disclosure of Invention

In order to solve the problems that the root system of rice is not developed and the stem leaves are not flourishing, the invention provides a method for preparing a selenium-rich rice stem cell growth promoter, which comprises the following steps: 1. preparing a compound organic selenium ionic liquid, 2, preparing a rice rooting agent, 3, preparing a rice terminal bud accelerant, 4, preparing a slow-release granular fertilizer, and 5, granulating at a low temperature and forming.

By adopting the technical scheme, the growth is rapid, the stems and leaves are luxuriant, the root system is developed, the disease resistance is strong, and the nutrition of the fruits is rich after the promoter is applied.

Disclosure of Invention

A method for preparing a selenium-rich rice stem cell growth promoter comprises the following steps:

1 preparation of compound organic selenium ionic liquid, 0.2-1% of sodium selenite, 5-15% of industrial glucose, 0.2-0.6% of potassium dihydrogen phosphate, 0.5-0.8% of dipotassium hydrogen phosphate, 2-5% of magnesium sulfate, 1-3% of peptone, 6-8% of pH regulation, 1-3 × 10 of inoculated candida utilis⁶CFU/ml, introducing sterile air with a volume of 6-8vvm, stirring at a speed of 80-100rpm, maintaining the temperature at 25-28 deg.C, and culturing for 48-96 hr to obtain fermentation liquid;

2. preparation of a rice rooting agent: adding 15-20% of soybean meal powder, 30-40% of chicken manure, 0.1-0.6% of magnesium chloride, 0.1-0.5% of potassium dihydrogen phosphate and 0.1-0.3% of dipotassium hydrogen phosphate, adding 40-60% of water, and adding 1-5% of 10%⁷CFU/ml Bacillus subtilis, 1-5% of 10⁷CFU/ml Bacillus licheniformis, 1-5% of 10⁶Fermenting the enterococcus faecalis in a concentration of CFU/ml at 25-27 deg.C for 3-5 days to obtain soybean meal hydrolysate;

3. preparing a rice terminal bud accelerant: 20-35 parts of bran, 30-50 parts of rice bran and 40-60% of added water,heating at 70 deg.C for 1 hr, adding naphthylacetic acid 0.2-0.4%, cooling, and adding 10%⁷2-5% of CFU trichoderma, 10% of CFU trichoderma⁷2-5% of CFU/ml acetic acid bacteria, fermenting at 20-25 deg.C for 1-2 days in the first stage, and fermenting at 28-32 deg.C for 2-3 days in the later stage;

4. preparing a slow release granular fertilizer, wherein 20-30% of diatomite, 10-15% of rice husk, 5-10% of modified corn starch, 10-35% of rooting agent, 15-20% of germination promoter and 5-10% of selenium-rich ionic liquid;

5. low-temperature extrusion molding, controlling the temperature of a particle molding device at 70-80 ℃, controlling the diameter of a die hole at 6-12mm, rapidly extruding, and drying until the water content is lower than 20%.

6 application method: the application amount from the seedling stage to the tillering stage is 10-300 kg/mu, and the application amount from the mature stage is 40-50 kg/mu.

The beneficial technical effects of the invention are as follows:

1. the fertilizer is suitable for rice growth, and has the advantages of fast growth of rice, developed root system, strong disease resistance, complete trace elements, high nutrient content in rice and selenium-rich effect.

2. Nutrition and parameters of rice

Table 1 rice nutrition parameters with application of promoters

TABLE 2 Rice parameter comparison

Detailed Description

The invention embodies the advantages mentioned above through the development of the embodiment

1. composite organic selenium ionPreparing solution from sodium selenite 1%, industrial glucose 15%, potassium dihydrogen phosphate 0.5%, dipotassium hydrogen phosphate 0.6%, magnesium sulfate 4%, peptone 3%, adjusting pH6, inoculating Candida utilis 2 x 10⁶CFU/ml, the introduction amount of sterile air is 8vvm, the stirring speed is 90rpm, the temperature is maintained at 29 ℃, and the culture is carried out for 96 hours to obtain fermentation liquor for later use;

2. preparation of a rice rooting agent: adding 20% soybean meal powder, 35% chicken manure, magnesium chloride 0.1-0.6%, potassium dihydrogen phosphate 0.1-0.5%, dipotassium hydrogen phosphate 0.1-0.3%, adding water 45%, and adding 1-5% 10%⁷CFU/ml Bacillus subtilis, 1-5% of 10⁷CFU/ml Bacillus licheniformis, 1-5% of 10⁶Fermenting the enterococcus faecalis at the temperature of 27 ℃ for 3 days by CFU/ml to generate a bean pulp hydrolysate;

3. preparing a rice terminal bud accelerant: 25 parts of bran and 35 parts of rice bran, adding 40% of water, heating at 70 ℃ for 1h, adding 0.2-0.4% of naphthylacetic acid, cooling, and adding 10% of⁷2-5% of CFU/ml trichoderma and 10% of CFU/ml trichoderma⁷2-5% of CFU/ml acetic acid bacteria, fermenting at 25 ℃ for 2 days in the early stage, and fermenting at 28 ℃ for 3 days in the later stage for later use;

4. preparing a slow-release granular fertilizer, namely 25% of diatomite, 10% of rice hull, 8% of modified corn starch, 30% of rooting agent, 15% of germination accelerator and 12% of selenium-rich ionic liquid;

5. and (3) carrying out low-temperature extrusion molding, controlling the temperature of a particle molding device to be 80 ℃, controlling the diameter of a die hole to be 10mm, and drying after rapid extrusion to ensure that the water content is lower than 20%.

6 application method: the application amount from the seedling stage to the tillering stage is 20 kg/mu, and the application amount from the mature stage is 40 kg/mu.

Claims

1. A method for preparing a selenium-rich rice stem cell growth promoter is characterized by comprising the following steps: 1. preparing a compound organic selenium ionic liquid, 2, preparing a rice rooting agent, 3, preparing a rice terminal bud accelerant, 4, preparing a slow-release granular fertilizer, and 5, granulating at a low temperature and forming.

2. The "stem cell growth promoter" according to claim 1, characterized in that: preparing a compound organic selenium ionic liquid, namely 0.2-1% of sodium selenite, 5-15% of industrial glucose, 0.2-0.6% of potassium dihydrogen phosphate, 0.5-0.8% of dipotassium hydrogen phosphate, 2-5% of magnesium sulfate, 1-3% of peptone, adjusting the pH value to 6-8, inoculating 1-3 × 106CFU/ml of candida utilis, introducing 6-8vvm of sterile air, stirring at a rotating speed of 80-100rpm, maintaining the temperature at 25-28 ℃, and culturing for 48-96 hours to obtain a fermentation liquid for later use;

3. the "stem cell growth promoter" according to claim 1, characterized in that: preparation of a rice rooting agent: adding 15-20% of soybean meal powder, 30-40% of chicken manure, 0.1-0.6% of magnesium chloride, 0.1-0.5% of potassium dihydrogen phosphate and 0.1-0.3% of dipotassium hydrogen phosphate, adding 40-60% of water, and adding 1-5% of 10%⁷CFU/ml Bacillus subtilis, 1-5% of 10⁷CFU/ml Bacillus licheniformis, 1-5% of 10⁶Fermenting the enterococcus faecalis in a concentration of CFU/ml at 25-27 deg.C for 3-5 days to obtain soybean meal hydrolysate;

4. the "stem cell growth promoter" according to claim 1, characterized in that: preparing a rice terminal bud accelerant: 20-35 parts of bran, 30-50 parts of rice bran, 40-60% of water, heating at 70 ℃ for 1h, adding 0.2-0.4% of naphthylacetic acid, cooling, and adding 10% of water⁷2-5% of CFU/ml trichoderma and 10% of CFU/ml trichoderma⁷2-5% of CFU/ml acetic acid bacteria, fermenting at 20-25 deg.C for 1-2 days in the first stage, and fermenting at 28-32 deg.C for 2-3 days in the later stage;

5. the "stem cell growth promoter" according to claim 1, characterized in that: preparing a slow release granular fertilizer, wherein 20-30% of diatomite, 10-15% of rice husk, 5-10% of modified corn starch, 10-35% of rooting agent, 15-20% of germination promoter and 5-10% of selenium-rich ionic liquid;

6. the "stem cell growth promoter" according to claim 1, characterized in that: extruding at low temperature, controlling the temperature of the particle forming device at 70-80 ℃ and the diameter of a die hole at 6-12mm, rapidly extruding, and drying until the water content is lower than 20%;

7. the application method comprises the following steps: the application amount from the seedling stage to the tillering stage is 10-300 kg/mu, and the application amount from the mature stage is 40-50 kg/mu.