CN110583460A

CN110583460A - Soilless culture method for okra

Info

Publication number: CN110583460A
Application number: CN201911018145.3A
Authority: CN
Inventors: 侯军亮; 何阳; 林春梅; 白会超
Original assignee: Hainan Run Da Modern Agriculture Group Ltd By Share Ltd
Current assignee: Hainan Run Da Modern Agriculture Group Ltd By Share Ltd
Priority date: 2019-10-24
Filing date: 2019-10-24
Publication date: 2019-12-20

Abstract

The invention relates to a soilless culture method of okra, which comprises the following steps: seed pretreatment, substrate seedling raising, field planting, growth period management, fruit setting and fruit harvesting; the adopted seedling culture substrate comprises: manioc waste, crop straws, liquorice, turfy soil, carbonized rice hulls, erythrina bark, green algae, spirogyra, river sand, sawdust, potassium feldspar powder, erigeron breviscapus, vinegar residue, modified kaolin, cassia obtusifolia branches, vermiculite, perlite, zeolite, organic fertilizer, humus, additives and compound microbial agents; the organic fertilizer comprises wormcast, cow dung and chicken dung; the humus comprises straw decomposing bacteria, humic acid and biogas residue fermentation material. According to the soilless culture method of the okra, the planted okra has high germination rate, the seedling period and the germination days are shorter than those of the prior art, and the freshness and the weight of seedlings and the yield are obviously improved.

Description

Soilless culture method for okra

Technical Field

The invention relates to the technical field of crop cultivation, in particular to a soilless culture method for okra.

Background

Okra, also called okra and coffee okra in the family of malvaceae, is characterized by being crisp, tender, juicy, smooth, not greasy and unique in flavor, and has become a popular vegetable in recent years.

The okra contains protein, fat, carbohydrate, abundant vitamin A and B groups, calcium, phosphorus, iron and the like, and trace elements such as selenium, zinc and the like, has certain help for enhancing the immunity of a human body, mainly has the effects of relieving sore throat, treating stranguria, promoting lactation, regulating menstruation and the like, and can also treat sore throat, color of urine, diabetes and the like.

Soilless culture is a modern seedling culture technology that plants are fixed by taking light materials such as grass peat or forest leaf mold, vermiculite and the like as seedling culture substrates, root systems of the plants are directly contacted with nutrient solution, and seedlings are formed at one time by mechanical precision seeding. The soilless seedling culture does not use soil, but uses solid materials which are not soil as a substrate, and is watered with nutrient solution, or does not use any substrate, but uses a water culture or spray mode to culture the seedlings, and has the following advantages:

the seedlings grow rapidly, the seedling age is short, the root system is well developed, the seedlings are strong and tidy, the seedling revival time after the planting is short, and the seedlings are easy to survive. The substrate seedling culture and the nutrient solution seedling culture can ensure sufficient water and nutrient supply and good substrate ventilation. Meanwhile, the soilless seedling culture is convenient for scientific and standard management.

Disclosure of Invention

The invention provides a soilless culture method of okra, which comprises the following steps:

step S1: pretreating seeds;

step S2: culturing seedlings by using a substrate;

step S3: planting;

step S4: managing in a growing period, and setting fruits and harvesting the fruits;

the seedling raising substrate adopted in the step S2 comprises the following components in parts by mass: 10-20 parts of cassava residue, 10-20 parts of crop straw, 10-20 parts of liquorice, 10-20 parts of turfy soil, 15-20 parts of carbonized rice hulls, 12-18 parts of erythrina bark, 8-12 parts of green algae, 4-6 parts of spirogyra, 8-12 parts of river sand, 15-25 parts of sawdust, 10-15 parts of potassium feldspar powder, 10-12 parts of erigeron breviscapus, 10-15 parts of vinegar residue, 14-16 parts of modified kaolin, 12-18 parts of cassia bismuthate branches, 20-30 parts of vermiculite, 25-30 parts of perlite, 10-15 parts of zeolite, 6-8 parts of organic fertilizer, 6-8 parts of humus, 8-12 parts of additive and 2-4 parts of composite microbial agent;

the organic fertilizer comprises the following components in parts by weight: 10-20 parts of wormcast, 10-20 parts of cow dung and 15-25 parts of chicken dung;

the humus comprises the following components in parts by mass: 6-10 parts of straw rotten bacteria, 8-16 parts of humic acid and 10-15 parts of biogas residue fermentation material.

The additive comprises the following components in parts by mass: 2-4 parts of vitamin complex, 2-4 parts of algae powder, 2-4 parts of rice chaff ash, 3-5 parts of magnesium stearate, 3-5 parts of sodium alginate, 4-6 parts of urea, 1-2 parts of ammonium dihydrogen phosphate, 1-2 parts of nano silver and 1-2 parts of nano carbon.

The compound microbial agent comprises the following components in parts by weight: 1-2 parts of lactic acid bacteria agent, 1-2 parts of photosynthetic bacteria agent, 3-6 parts of bacillus subtilis agent, 4-5 parts of saccharomycete agent, 4-5 parts of potassium bacteria agent and 2-4 parts of filamentous bacteria agent.

The preparation method of the seedling substrate comprises the following steps:

step S21: placing kaolin into 10% hydrochloric acid solution to be soaked for 5 minutes, keeping the temperature at room temperature, and then washing the kaolin with deionized water; soaking the seeds in 5% sulfuric acid solution for five minutes at room temperature, and then washing the seeds with deionized water; soaking the mixture in 5% phosphoric acid solution for five minutes, keeping the temperature at room temperature, and then washing the mixture with deionized water; finally, soaking the glass fiber in 10% sodium hydroxide solution for ten minutes, and then washing the glass fiber with deionized water;

step S22: drying the kaolin obtained in the step S21 until the water content is between 20 and 30 percent, calcining the kaolin at high temperature for 1 hour, taking the kaolin out, grinding and crushing the kaolin to obtain modified kaolin;

step S23: pulverizing erythrina bark and cassia tora branches, and heating and extracting for 10 minutes by using distilled water, wherein an enzyme preparation formed by mixing cellulase, pectinase and tannase is added into the distilled water to obtain an extract;

step S24: crushing and sieving crop straws, vermiculite, perlite and zeolite;

step S25: mixing manioc waste, liquorice, turfy soil, carbonized rice hulls, green algae, spirogyra, river sand, sawdust, potassium feldspar powder, erigeron breviscapus, vinegar residue, organic fertilizer and additives with the materials obtained in the step S22-the step S24 uniformly;

step S26: sealing for 3-5 days, inoculating the compound microbial agent when the temperature is reduced to room temperature, adding humus, turning and mixing uniformly, sealing and fermenting for 10-13 days, and controlling the temperature of the material to be 35-45 degrees centigrade to obtain the seedling culture substrate.

Wherein, the step S1 specifically includes the following steps:

step S11: selecting plump seeds, and soaking in 0.1-0.2% thymol water solution for 1 hr to restore seed activity;

step S12: soaking the seeds obtained in the step S11 in a disinfectant, wherein the disinfectant comprises the following components in parts by weight: 5-8 parts of copper sulfate, 6-8 parts of copper hydroxide, 2-4 parts of calcium hydroxide, 1-2 parts of potassium permanganate and 20-30 parts of hydrogen peroxide;

step S13: soaking the seeds obtained in the step S12 with a seed soaking solution, wherein the seed soaking solution comprises the following components in parts by mass: 3-5 parts of maltose, 1-2 parts of wettable rooting agent, 1-2 parts of ferrous sulfate, 2-6 parts of sodium citrate, 1-2 parts of gibberellin, 1-2 parts of sodium dihydrogen phosphate, 2-5 parts of glycine and 20-30 parts of hydrogen peroxide;

step S14: and (4) mixing the seeds obtained in the step (S13) with a germination accelerating substrate, placing the mixture in a breathable gauze bag, and placing the gauze bag at room temperature for 10-12 days, wherein the adopted germination accelerating substrate comprises the following components in parts by weight: 1-2 parts of zymophyte, 6-10 parts of modified kaolin, 6-8 parts of fine sand and 5-6 parts of borax;

step S15: puncturing one end of the seed obtained in the step S14, far away from the germ, with a needle to break the epidermis of the seed, and then soaking the seed in a mixed growth regulator for one hour, wherein the mixed growth regulator comprises the following components in parts by weight: 1-2 parts of naphthylacetic acid, 61-2 parts of vitamin B, 1-2 parts of rooting powder and 10-20 parts of hydrogen peroxide;

step S16: cleaning the seeds with clear water, standing at room temperature, and air drying to obtain the seeds to be grown.

In step S14, the preparation method of the modified kaolin includes: placing kaolin into 10% hydrochloric acid solution to be soaked for 5 minutes, keeping the temperature at room temperature, and then washing the kaolin with deionized water; soaking the mixture in 5% sulfuric acid solution at room temperature for five minutes, and washing with deionized water; soaking the mixture in 5% phosphoric acid solution for five minutes, keeping the temperature at room temperature, and then washing the mixture with deionized water; finally, the mixture is put into 10 percent sodium hydroxide solution to be soaked for ten minutes and then is washed clean by deionized water; and finally, drying the obtained kaolin until the water content is between 20 and 30 percent, calcining the kaolin for 1 hour at high temperature, taking the kaolin out, grinding and crushing the kaolin to obtain the modified kaolin.

Wherein, in the step S3, the nutrient solution is applied every 5-8 days.

The applied nutrient solution comprises the following components in parts by mass: 10-12 parts of biogas slurry, 1-2 parts of urea, 801-2 parts of tween-801, 6-8 parts of casein, 1-2 parts of sodium dihydrogen phosphate, 1-2 parts of calcium nitrate and 30-40 parts of deionized water.

According to the soilless culture method of the okra, the planted okra has high germination rate, the seedling culture period and the germination days are shorter than those of the prior art, and the fresh weight and the yield of seedlings are obviously improved.

Detailed Description

In order to further understand the technical solution and the advantages of the present invention, the following detailed description will be provided for the technical solution and the advantages thereof.

The invention provides a soilless culture method of okra, which aims to improve germination rate, germination accelerating rate, vitality index and yield, starts with two major parts of pretreatment of seeds and components of a matrix, and improves the process of soilless culture and required components, and specifically comprises the following steps:

seed pretreatment

1. Selecting plump seeds, soaking in 0.1-0.2% thymol water solution for 1 hr to restore seed activity.

2. Soaking the seeds with a disinfectant, wherein the disinfectant comprises the following components in parts by weight: 5-8 parts of copper sulfate, 6-8 parts of copper hydroxide, 2-4 parts of calcium hydroxide, 1-2 parts of potassium permanganate and 20-30 parts of hydrogen peroxide; the disinfectant can prevent damage of plant diseases and insect pests to the okra from the source, so that the yield of the okra is increased, and the disinfection time is not limited by the invention and is preferably 15-30 min.

3. Soaking the seeds with a seed soaking solution, wherein the seed soaking solution comprises the following components in parts by mass: 3-5 parts of maltose, 1-2 parts of wettable rooting agent, 1-2 parts of ferrous sulfate, 2-6 parts of sodium citrate, 1-2 parts of gibberellin, 1-2 parts of sodium dihydrogen phosphate, 2-5 parts of glycine and 20-30 parts of hydrogen peroxide; the seed soaking process is preferably carried out in a silica gel container, the seed soaking time is between 20 and 40min, the volume of the seed soaking liquid is preferably just over the surface of the seeds, the seed soaking liquid can coordinate the physiological metabolism of the seeds, the germination rate of the seeds is improved, meanwhile, the full release of growth factors and nutrient substances in the embryos is promoted, and a foundation is laid for the subsequent seedling culture and field planting.

4. Mixing the obtained seeds with a germination accelerating matrix, placing the mixture in a breathable gauze bag, and placing the gauze bag at room temperature for 10-12 days, wherein the adopted germination accelerating matrix comprises the following components in parts by weight: 1-2 parts of zymophyte, 6-10 parts of modified kaolin, 6-8 parts of fine sand and 5-6 parts of borax; the germination accelerating temperature is higher than room temperature, preferably between 30 and 40 degrees centigrade, wherein the preparation method and the beneficial effects of the modified kaolin are detailed later, the germination accelerating substrate can break the dormant state of seeds, meanwhile, the addition of zymophyte and the modified kaolin can fully stimulate the seeds, reduce the time of germination accelerating treatment and improve the germination accelerating efficiency, and the germination of the seed embryo treated by the germination accelerating substrate is neat in germination, high in emergence rate and high in germination accelerating rate in the later planting process.

5. Puncturing one end of the obtained seed, far away from the germ, with a needle to break the epidermis of the seed, then soaking the seed in a mixed growth regulator for one hour to ensure the absorption of the germ on the growth regulator, simultaneously ensuring the emergence rate in the subsequent substrate seedling raising process, and shortening the emergence time, wherein the mixed growth regulator comprises the following components in parts by mass: 1-2 parts of naphthylacetic acid, 61-2 parts of vitamin B, 1-2 parts of rooting powder and 10-20 parts of hydrogen peroxide; the mixed growth regulator can obviously improve the germination rate of seeds and the vitality index of embryos.

6. Cleaning the seeds with clear water, standing at room temperature, and air drying to obtain the seeds to be grown.

Second, substrate seedling raising

The seedling raising substrate adopted by the invention comprises the following components in parts by mass: 10-20 parts of cassava residue, 10-20 parts of crop straw, 10-20 parts of liquorice, 10-20 parts of turfy soil, 15-20 parts of carbonized rice hulls, 12-18 parts of erythrina bark, 8-12 parts of green algae, 4-6 parts of spirogyra, 8-12 parts of river sand, 15-25 parts of sawdust, 10-15 parts of potassium feldspar powder, 10-12 parts of erigeron breviscapus, 10-15 parts of vinegar residue, 14-16 parts of modified kaolin, 12-18 parts of cassia bismutha branches, 20-30 parts of vermiculite, 25-30 parts of perlite, 10-15 parts of zeolite, 6-8 parts of organic fertilizer, 6-8 parts of humus, 8-12 parts of additive and 2-4 parts of composite microbial agent.

According to the invention, river sand, sawdust, cassava residue and crop straws are used for replacing a part of vermiculite, perlite and the like, so that on one hand, waste can be recycled, the cost is reduced, on the other hand, the air permeability of a seedling culture substrate can be increased, the substrate is loose and breathable, the fertility is durable, the survival and growth of seeds are facilitated, and the composite microbial inoculum can exert the maximum effect. Compared with the traditional Chinese medicine, the addition of the liquorice and the erigeron breviscapus is found by the inventor to improve the germination rate of the seeds; the compound microbial agent has the functions of inhibiting the growth of microbes and reducing diseases and pests.

In the invention, the organic fertilizer comprises the following components in parts by weight: 10-20 parts of wormcast, 10-20 parts of cow dung and 15-25 parts of chicken manure, the raw materials of the organic fertilizer are cheap and easy to obtain, and the fertilizer efficiency and nutrients of the substrate can be maintained for a long time, so that the later-stage okra grows healthily and the yield is improved.

The humus comprises the following components in parts by mass: 6-10 parts of straw rotten bacteria, 8-16 parts of humic acid and 10-15 parts of biogas residue fermentation material, wherein the humic acid can inhibit the breeding of microorganisms and prevent other harmful substances from damaging seeds, meanwhile, the existence of the humic acid can keep the pH value of the matrix in a stable state, and the humic acid and organic fertilizer play an antagonistic role, so that the root burning phenomenon caused by insufficient nutrition of the seeds in the field planting process is avoided.

The additive comprises the following components in parts by mass: 2-4 parts of vitamin complex, 2-4 parts of algae powder, 2-4 parts of rice chaff ash, 3-5 parts of magnesium stearate, 3-5 parts of sodium alginate, 4-6 parts of urea, 1-2 parts of ammonium dihydrogen phosphate, 1-2 parts of nano silver and 1-2 parts of nano carbon. The additive covers most of substances required by the growth and development of seeds, and can enable the okra to have developed root system, shortened growth period, long growth period and strong disease resistance; moreover, the nano carbon is added, so that the water absorption capacity of the root system of the seed is further ensured, and the realization of the water-saving effect in the planting process is facilitated; the addition of the nano silver further enhances the disease resistance of the seeds, and facilitates the management of the growth period in the later planting process and the like.

The compound microbial agent comprises the following components in parts by weight: 1-2 parts of lactic acid bacteria agent, 1-2 parts of photosynthetic bacteria agent, 3-6 parts of bacillus subtilis agent, 4-5 parts of saccharomycete agent, 4-5 parts of potassium bacteria agent and 2-4 parts of filamentous bacteria agent. The potassium-dissolving bacterium agent is mainly used for acting on potassium feldspar powder in a matrix to continuously release potassium elements so as to keep certain growth activity of seeds.

The preparation method of the seedling substrate comprises the following steps:

1. soaking kaolin in 10% hydrochloric acid solution for 5 min, keeping the temperature at room temperature, and then washing with deionized water; soaking the seeds in 5% sulfuric acid solution for five minutes, keeping the temperature at room temperature, and then washing the seeds with deionized water; soaking the mixture in 5% phosphoric acid solution for five minutes at room temperature, and washing the mixture with deionized water; finally, the glass is soaked in 10% sodium hydroxide solution for ten minutes and then is washed clean by deionized water.

2. Drying the kaolin until the water content is between 20 and 30 percent, calcining the kaolin for 1 hour at high temperature, taking out the kaolin, grinding and crushing the kaolin to obtain the modified kaolin. The surface acid value of the modified kaolin is reduced, which is beneficial to keeping various nutrient components in the matrix from losing, so that the seeds are in a relatively stable breeding environment. When the kaolin is modified, the acidity of the acid liquor is gradually reduced to achieve the optimal modification effect, and meanwhile, the finally obtained modified kaolin is mild in performance and convenient in post-treatment, and the integral acid-base balance of the seedling substrate is not damaged.

3. Pulverizing bark of erythrina indica lam and branches of Cassia occidentalis, and extracting with distilled water under heating for 10 min, wherein enzyme preparation prepared by mixing cellulase, pectinase and tannase is added into distilled water to obtain extractive solution. Cellulase and pectinase can increase the wall breaking efficiency and the extraction efficiency, are favorable for the full release of nutrient solution, and the single tannase can reduce the tannin content of the matrix, avoid tannin and protein from forming a complex and improve the nutritional value of the matrix.

4. Crushing and sieving crop straw, vermiculite, perlite and zeolite.

5. Mixing manioc waste, liquorice, turfy soil, carbonized rice hulls, green algae, spirogyra, river sand, sawdust, potassium feldspar powder, erigeron breviscapus, vinegar residue, organic fertilizer and additives with the materials obtained in the step uniformly.

6. Sealing for 3-5 days, inoculating the compound microbial agent when the temperature is reduced to room temperature, adding the humus, turning and mixing uniformly, sealing and fermenting for 10-13 days, and controlling the temperature of the materials to be 35-45 ℃ to obtain the seedling culture substrate.

Thirdly, planting

After the seeds sprout and are white, planting, and applying nutrient solution every 5-8 days; the applied nutrient solution comprises the following components in parts by mass: 10-12 parts of biogas slurry, 1-2 parts of urea, 801-2 parts of tween-2, 6-8 parts of casein, 1-2 parts of sodium dihydrogen phosphate, 1-2 parts of calcium nitrate and 30-40 parts of deionized water.

Fourth, management in growing period, fruit setting and fruit harvesting

Other detailed steps involved in the processes of field planting, growth period management, fruit setting and fruit harvesting and the like can be referred to the prior art, which is not the protection focus of the invention, and the invention is not repeated herein.

In order to verify the beneficial effects of the soilless culture method provided by the invention, the invention provides the following specific examples, the detailed operation steps in each example are described in detail above, and the differences only lie in the changes of the components of the substances involved in each step, which are specifically as follows:

1. seed pretreatment

Table 1: examples ingredients of matter involved in seed pretreatment

2. Seedling stage

Table 2: the material components related to the seedling raising substrate of each embodiment

3. Stage of permanent planting

Table 3: ingredients of substances involved in colonization of each example

	Example 1	Example 2	Example 3	Example 4
					Biogas slurry	10	11	11	12
Urea	1	2	1	2
					Tween-80	1	1	2	2
Casein as a food additive	8	8	7	6
					Sodium dihydrogen phosphate	2	2	2	1
Calcium nitrate	2	1	2	1
					Deionized water	30	35	38	40

Comparative example 1: the seeds are not pretreated, the seedling substrate adopts the existing seedling substrate on the market, the nutrient solution applied after the planting is also the existing nutrient solution on the market, besides, the processes of the planting, the growth period management, the fruit setting and the fruit harvest are the same as the invention, and the planting results are as follows:

table 4: comparison of cultivation effects of examples and comparative examples

As can be seen from Table 4, the soilless culture method for okra provided by the invention has the advantages that the germination rate of the cultured okra is high, the seedling culture period and the germination days are shorter than those of the prior art, and the fresh weight and the yield of seedlings are obviously improved.

Although the present invention has been described with reference to the preferred embodiments, it should be understood that the scope of the present invention is not limited thereto, and those skilled in the art will appreciate that various changes and modifications can be made without departing from the spirit and scope of the present invention.

Claims

1. A soilless culture method of okra is characterized by comprising the following steps:

step S1: pretreating seeds;

step S2: culturing seedlings by using a substrate;

step S3: planting;

2. A soilless culture method for okra according to claim 1, wherein the additive comprises the following components in parts by mass: 2-4 parts of vitamin complex, 2-4 parts of algae powder, 2-4 parts of rice chaff ash, 3-5 parts of magnesium stearate, 3-5 parts of sodium alginate, 4-6 parts of urea, 1-2 parts of ammonium dihydrogen phosphate, 1-2 parts of nano silver and 1-2 parts of nano carbon.

3. The soilless culture method of okra according to claim 1, wherein the complex microbial inoculant comprises the following components in parts by mass: 1-2 parts of lactic acid bacteria agent, 1-2 parts of photosynthetic bacteria agent, 3-6 parts of bacillus subtilis agent, 4-5 parts of saccharomycete agent, 4-5 parts of potassium bacteria agent and 2-4 parts of filamentous bacteria agent.

4. A soilless culture method of okra according to claim 1, wherein the preparation method of said seedling substrate comprises the following steps:

step S21: placing kaolin into 10% hydrochloric acid solution to be soaked for 5 minutes, keeping the temperature at room temperature, and then washing the kaolin with deionized water; soaking the seeds in 5% sulfuric acid solution for five minutes, keeping the temperature at room temperature, and then washing the seeds with deionized water; soaking the mixture in 5% phosphoric acid solution for five minutes, keeping the temperature at room temperature, and then washing the mixture with deionized water; finally, soaking the glass fiber in 10% sodium hydroxide solution for ten minutes, and then washing the glass fiber with deionized water;

step S23: pulverizing erythrina bark and cassia tora branches, and heating and extracting for 10 minutes by using distilled water, wherein an enzyme preparation formed by mixing cellulase, pectinase and tannase is added into the distilled water to obtain an extracting solution;

step S24: crushing and sieving crop straws, vermiculite, perlite and zeolite;

step S26: sealing for 3-5 days, inoculating the compound microbial agent when the temperature is reduced to room temperature, adding humus, turning and mixing uniformly, sealing and fermenting for 10-13 days, and controlling the temperature of the materials to be 35-45 ℃ to obtain the seedling culture substrate.

5. A soilless culture method for okra according to claim 1, wherein said step S1 specifically comprises the steps of:

step S14: mixing the seeds obtained in the step S13 with a germination accelerating matrix, placing the mixture in a breathable gauze bag, and placing the bag for 10-12 days at room temperature, wherein the adopted germination accelerating matrix comprises the following components in parts by weight: 1-2 parts of zymophyte, 6-10 parts of modified kaolin, 6-8 parts of fine sand and 5-6 parts of borax;

6. A soilless culture method for okra as claimed in claim 1, wherein: in the step S14, the preparation method of the modified kaolin includes: placing kaolin into 10% hydrochloric acid solution to be soaked for 5 minutes, keeping the temperature at room temperature, and then washing the kaolin with deionized water; soaking the seeds in 5% sulfuric acid solution for five minutes, keeping the temperature at room temperature, and then washing the seeds with deionized water; soaking the mixture in 5% phosphoric acid solution for five minutes, keeping the temperature at room temperature, and then washing the mixture with deionized water; finally, soaking the glass fiber in 10% sodium hydroxide solution for ten minutes, and then washing the glass fiber with deionized water; and finally, drying the obtained kaolin until the water content is between 20 and 30 percent, calcining the kaolin for 1 hour at high temperature, taking the kaolin out, grinding the kaolin and crushing the kaolin to obtain the modified kaolin.

7. A soilless culture method for okra as claimed in claim 1, wherein: in the step S3, nutrient solution is applied at intervals of 5-8 days.

8. A soilless culture method for okra as claimed in claim 7, wherein: the applied nutrient solution comprises the following components in parts by mass: 10-12 parts of biogas slurry, 1-2 parts of urea, 801-2 parts of tween-2, 6-8 parts of casein, 1-2 parts of sodium dihydrogen phosphate, 1-2 parts of calcium nitrate and 30-40 parts of deionized water.