CN113832570A - Antibacterial and antiviral nanofiber and preparation method thereof - Google Patents

Antibacterial and antiviral nanofiber and preparation method thereof Download PDF

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Publication number
CN113832570A
CN113832570A CN202110962752.6A CN202110962752A CN113832570A CN 113832570 A CN113832570 A CN 113832570A CN 202110962752 A CN202110962752 A CN 202110962752A CN 113832570 A CN113832570 A CN 113832570A
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antibacterial
antiviral
nanofiber
preparing
nanofibers
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丛海林
于冰
郭帅兵
申有青
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Qingdao University
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Qingdao University
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    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
    • D01F8/00—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof
    • D01F8/04—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from synthetic polymers
    • D01F8/08—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from synthetic polymers with at least one polyacrylonitrile as constituent
    • A—HUMAN NECESSITIES
    • A41—WEARING APPAREL
    • A41D—OUTERWEAR; PROTECTIVE GARMENTS; ACCESSORIES
    • A41D13/00—Professional, industrial or sporting protective garments, e.g. surgeons' gowns or garments protecting against blows or punches
    • A—HUMAN NECESSITIES
    • A41—WEARING APPAREL
    • A41D—OUTERWEAR; PROTECTIVE GARMENTS; ACCESSORIES
    • A41D13/00—Professional, industrial or sporting protective garments, e.g. surgeons' gowns or garments protecting against blows or punches
    • A41D13/05—Professional, industrial or sporting protective garments, e.g. surgeons' gowns or garments protecting against blows or punches protecting only a particular body part
    • A41D13/11—Protective face masks, e.g. for surgical use, or for use in foul atmospheres
    • A41D13/1192—Protective face masks, e.g. for surgical use, or for use in foul atmospheres with antimicrobial agent
    • A—HUMAN NECESSITIES
    • A41—WEARING APPAREL
    • A41D—OUTERWEAR; PROTECTIVE GARMENTS; ACCESSORIES
    • A41D31/00—Materials specially adapted for outerwear
    • A41D31/04—Materials specially adapted for outerwear characterised by special function or use
    • A41D31/30—Antimicrobial, e.g. antibacterial
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
    • D01D1/00—Treatment of filament-forming or like material
    • D01D1/02—Preparation of spinning solutions
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
    • D01D5/00—Formation of filaments, threads, or the like
    • D01D5/0007—Electro-spinning
    • D01D5/0015—Electro-spinning characterised by the initial state of the material
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
    • D01F8/00—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof
    • D01F8/04—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from synthetic polymers
    • D01F8/16—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from synthetic polymers with at least one other macromolecular compound obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds as constituent

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  • Engineering & Computer Science (AREA)
  • Textile Engineering (AREA)
  • Mechanical Engineering (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Artificial Filaments (AREA)
  • Nonwoven Fabrics (AREA)

Abstract

The invention discloses an antibacterial and antiviral nanofiber and a preparation method thereof. The antibacterial and antiviral nanofiber is prepared from poly (tetrahydropyrimidine), polyacrylonitrile and N, N-dimethylformamide, and the poly (tetrahydropyrimidine) and the polyacrylonitrile are dissolved in the N, N-dimethylformamide by the method. And preparing the mixed solution into the nano-fibers by an electrostatic spinning method. The method has the advantages of simple process flow, high production efficiency, low production cost and easy large-scale production, and the prepared nano-fiber has good antibacterial and antiviral effects and extremely low cytotoxicity and is very suitable for preparing protective articles such as masks, protective clothing and the like.

Description

Antibacterial and antiviral nanofiber and preparation method thereof
Technical Field
The invention relates to the technical field of antibacterial and antiviral nanofibers, and particularly relates to a method for preparing antibacterial and antiviral nanofibers by utilizing good antibacterial and antiviral effects of poly (tetrahydropyrimidine) and good mechanical properties of polyacrylonitrile.
Background
Health is an inevitable topic for all people. With the progress of the times, people in the 21 st century gradually solve the most basic demand problem of human beings, namely, the problem of satiety, so that higher requirements on the living standard are met. Therefore, the safety demand rises year by year and becomes a new pet of the times. Pathogenic bacteria such as escherichia coli, staphylococcus aureus and the like are common in life, and once invading a human body, symptoms such as inflammation, asthma and the like can be caused. And in recent years, new crown diseases have been abused, which brings more attention to health of people.
The nanofiber not only has efficient antibacterial and antiviral properties, but also has good mechanical properties and good adsorption properties, so that bacteria and viruses around the body of people can be well adsorbed, and the bacteria and the viruses lose activity by combining the properties of the poly-tetrahydropyrimidine. The polytetrahydropyrimidine showed no significant cytotoxicity in the cytotoxicity test. And the prepared antibacterial and antiviral nano-fiber has simple preparation process and low production cost, and is very suitable for textile articles such as an adsorption layer of a mask, protective clothing and the like.
The preparation of antibacterial and antiviral nanofibers is rarely studied internationally, and related patents are reported, and some documents have adopted the following preparation methods: (1) qiu et al reported in Journal 2020, 384, 123241 of Chemical Engineering Journal that triclosan, a good antimicrobial drug, was blended with polyacrylonitrile to spin on the surface of cotton fibers, and made antibacterial sportswear from this fabric, the antibacterial activity of sportswear became more durable after the functional nanofibers were embedded in the fabric; (2) kim et al in ACS Applied Materials&Journal 2021, 13, 857-. The air filter layer can effectively capturePM in air1Particles (diameter less than 1.0 μm). In addition, the heating layer is a copper microfiber pad, which has good mechanical properties, enabling the hybrid air filter to generate temperatures above 100 ℃, rendering bacteria and viruses non-productive at high temperatures, and the insulating layer prevents heat transfer to the other side. Such hybrid air filters offer great promise for antibacterial and antiviral protection; (3) karagoz et al in ACS Applied Materials&Interfaces journal 2021, 13, 5678-. The multifunctional material is prepared by electrospinning a poly (methyl methacrylate) (PMMA) solution containing zinc oxide nanorods and argon nanoparticles, has antiviral, antibacterial, self-cleaning and SERS activities, and is potentially useful for protective clothing applications. The preparation methods listed above need to utilize external conditions such as sunlight, air and the like to realize the antibacterial and antiviral effects, and have certain damage to human bodies, thereby limiting the practical production application of the antibacterial and antiviral agents.
Disclosure of Invention
Therefore, the invention aims to solve the problem of providing the antibacterial and antiviral nanofiber and the preparation method thereof, the method is simple in process flow, high in production efficiency, low in production cost and easy for large-scale production, and the prepared nanofiber has good antibacterial and antiviral effects and extremely low cytotoxicity and is very suitable for preparing protective articles such as masks and protective clothing.
In order to achieve the purpose of the invention, the following technical scheme is adopted in the application:
the antibacterial and antiviral nanofiber is prepared from the following raw materials:
0.5-2 parts of poly (tetrahydropyrimidine);
1-2 parts of polyacrylonitrile;
6-20 parts of N, N-dimethylformamide.
The antibacterial and antiviral nanofiber is prepared from the following raw materials:
1.4-1.6 parts by weight of poly (tetrahydropyrimidine);
1.4-1.6 parts of polyacrylonitrile;
5-9 parts of N, N-dimethylformamide.
A preparation method of antibacterial and antiviral nano-fiber comprises the following steps:
adding the poly-tetrahydropyrimidine and the polyacrylonitrile into N, N-dimethylformamide according to the designed amount, stirring and dissolving to prepare dispersion liquid, and preparing the antibacterial and antiviral nano-fiber from the dispersion liquid by adopting an electrostatic spinning method.
The stirring and dissolving is carried out for 4 to 10 hours at the temperature of between 30 and 100 ℃ by adopting magnetic stirring to obtain a dark brown transparent mixed solution.
Preferably, the stirring dissolution is carried out at 50-70 ℃ for 5-8 hours under the condition of magnetic stirring.
The voltage of the electrostatic spinning device is 5-20kv, and the flow rate of the injection pump is 5-25 microliter/min.
The distance between the needle head of the electrostatic spinning device and the roller is 2-30cm, the rotating speed of the roller is 50-500 r/min, and the antibacterial and antiviral nano-fiber with a good fiber structure and a uniform diameter is prepared.
The distance between the electrostatic spinning needle and the collecting roller is 10-20cm, and the rotating speed of the roller is 100-350 revolutions per minute.
And (3) absorbing the dispersion liquid by using an injector, and fixing the dispersion liquid on an injection pump device, wherein the flow rate of the injection pump is 10-20 microliter/minute, and the voltage of a high-voltage power supply is 10-17 kv.
Starting the electrostatic spinning device, and obtaining the antibacterial and antiviral nanofiber with high mechanical strength after 10 hours.
An application of the antibacterial and antiviral nanofiber in preparing protective clothing or masks.
Compared with the prior art, the invention has the advantages that:
(1) the method has the advantages of simple process equipment, good repeatability, low raw material price and obvious low cost advantage;
(2) the antibacterial and antiviral nano-fiber prepared by the method has strong inhibition effect on escherichia coli and staphylococcus aureus, and can play a good antibacterial effect;
(3) the antibacterial and antiviral nanofiber prepared by the method shows good cell compatibility to Hela and 3T3 cells, which indicates that the antibacterial and antiviral nanofiber can not cause harm to human bodies;
(4) the antibacterial and antiviral nano-fiber prepared by the method can inhibit the transfection of viruses to a great extent and has good antiviral effect;
drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a schematic view of an electrospinning apparatus according to the present application.
FIG. 2 is the scanning electron microscope test chart of the antibacterial and antiviral nanofibers obtained in example 3.
FIG. 3 is the scanning electron microscope test chart of the antibacterial and antiviral nanofibers obtained in example 9.
FIG. 4 is a graph showing the inhibitory effect of the antibacterial and antiviral nanofibers obtained in example 3 on Escherichia coli.
FIG. 5 is a graph showing the inhibitory effect of the antibacterial and antiviral nanofibers obtained in example 9 on Escherichia coli.
FIG. 6 is a graph showing the inhibitory effect of the antibacterial and antiviral nanofibers obtained in example 3 on Staphylococcus aureus.
FIG. 7 is a graph showing the inhibitory effect of the antibacterial and antiviral nanofibers obtained in example 9 on Staphylococcus aureus.
FIG. 8 is an optical microscope photograph of Hela cells incubated with the antibacterial and antiviral nanofibers obtained in example 3.
FIG. 9 is an optical microscope photograph of Hela cells incubated with the antibacterial and antiviral nanofibers obtained in example 9.
FIG. 10 is an optical microscope photograph of 3T3 cells incubated with the antibacterial and antiviral nanofibers obtained from example 3.
FIG. 11 is an optical microscope photograph of 3T3 cells incubated with the antibacterial and antiviral nanofibers obtained from example 9.
FIG. 12 is a fluorescent microscope photograph of the lentivirus cultured with the antibacterial and antiviral nanofibers obtained in example 3.
FIG. 13 is a fluorescent microscope photograph of the antibacterial and antiviral nanofiber-cultured lentivirus obtained in example 9.
1-high voltage power supply, 2-injection pump, 3-injector, 4-collecting roller and 5-needle.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Scanning electron micrographs were determined by Zeiss Sigma 500 scanning electron microscope.
The antibacterial and antiviral nanofiber is prepared from the following raw materials:
0.5-2 parts of poly (tetrahydropyrimidine);
1-2 parts of polyacrylonitrile;
5-20 parts of N, N-dimethylformamide.
The antibacterial and antiviral nanofiber is prepared from the following raw materials:
1.4-1.6 parts by weight of poly (tetrahydropyrimidine);
1.4-1.6 parts of polyacrylonitrile;
7-9 parts of N, N-dimethylformamide.
A preparation method of antibacterial and antiviral nano-fiber comprises the following steps:
adding the poly-tetrahydropyrimidine and the polyacrylonitrile into N, N-dimethylformamide according to the designed amount, stirring and dissolving to prepare dispersion liquid, and preparing the antibacterial and antiviral nano-fiber from the dispersion liquid by adopting an electrostatic spinning method.
The stirring and dissolving is carried out for 4 to 10 hours at the temperature of between 30 and 100 ℃ by adopting magnetic stirring to obtain a dark brown transparent mixed solution.
Preferably, the stirring dissolution is carried out at 50-70 ℃ for 5-8 hours under the condition of magnetic stirring.
The voltage of the electrostatic spinning device is 5-20kv, and the flow rate of the injection pump is 5-25 microliter/min.
The distance between the needle head of the electrostatic spinning device and the roller is 2-30cm, the rotating speed of the roller is 50-500 r/min, and the antibacterial and antiviral nano-fiber with a good fiber structure and a uniform diameter is prepared.
The distance between the electrostatic spinning needle and the collecting roller is 10-20cm, and the rotating speed of the roller is 100-350 revolutions per minute.
And (3) absorbing the dispersion liquid by using an injector, and fixing the dispersion liquid on an injection pump device, wherein the flow rate of the injection pump is 10-20 microliter/minute, and the voltage of a high-voltage power supply is 10-17 kv.
Starting the electrostatic spinning device shown in fig. 1, the schematic diagram of the electrostatic spinning device comprises a high-voltage power supply 1, an injection pump 2, an injector 3, a collecting roller 4 and a needle head 5. After 10 hours, the antibacterial and antiviral nano-fiber with high mechanical strength is obtained.
An application of the antibacterial and antiviral nanofiber in preparing protective clothing or masks.
Example 1
A preparation method of antibacterial and antiviral nano-fiber comprises the following steps:
(1) 0.5g of poly (tetrahydropyrimidine) and 1g of polyacrylonitrile and 5g of N, N-dimethylformamide were weighed and added to a 50mL round-bottomed flask, and stirred at 30 ℃ for 10 hours to obtain a transparent solution.
(2) Adding the transparent solution into a syringe, fixing the syringe on a syringe pump device, enabling the distance between a needle head and a collecting roller device to be 10cm, the rotating speed of the roller to be 100 r/min, the flowing speed of the syringe pump to be 16 microlitres/min, and the voltage of a high-voltage power supply to be 15 kv.
(3) Starting the electrostatic spinning device, and obtaining the antibacterial and antiviral nano-fiber after 10 hours.
Example 2
A preparation method of antibacterial and antiviral nano-fiber comprises the following steps:
(1) 0.5g of poly (tetrahydropyrimidine), 1.4g of polyacrylonitrile and 9g of N, N-dimethylformamide were weighed into a 50mL round-bottomed flask, and stirred at 50 ℃ for 5 hours to obtain a transparent solution.
(2) Adding the transparent solution into a syringe, fixing the syringe on a syringe pump device, enabling the distance between a needle head and a collecting roller device to be 15cm, the rotating speed of the roller to be 250 revolutions per minute, the flowing speed of the syringe pump to be 16 microliters per minute, and the voltage of a high-voltage power supply to be 15 kv.
(3) Starting the electrostatic spinning device, and obtaining the antibacterial and antiviral nano-fiber after 10 hours.
Example 3
A preparation method of antibacterial and antiviral nano-fiber comprises the following steps:
(1) 0.5g of poly (tetrahydropyrimidine) and 2g of polyacrylonitrile and 20g of N, N-dimethylformamide were weighed and added to a 50mL round-bottomed flask, and stirred at 100 ℃ for 3 hours to obtain a transparent solution.
(2) Adding the transparent solution into a syringe, fixing the syringe on a syringe pump device, enabling the distance between a needle head and a collecting roller device to be 15cm, the rotating speed of the roller to be 250 revolutions per minute, the flowing speed of the syringe pump to be 16 microliters per minute, and the voltage of a high-voltage power supply to be 15 kv.
(3) Starting the electrostatic spinning device, and obtaining the antibacterial and antiviral nano-fiber after 10 hours.
Example 4
A preparation method of antibacterial and antiviral nano-fiber comprises the following steps:
(1) 1.5g of poly (tetrahydropyrimidine) and 1g of polyacrylonitrile and 5g of N, N-dimethylformamide were weighed and added to a 50mL round-bottomed flask, and stirred at 30 ℃ for 10 hours to obtain a transparent solution.
(2) Adding the transparent solution into a syringe, fixing the syringe on a syringe pump device, enabling the distance between a needle head and a collecting roller device to be 15cm, the rotating speed of the roller to be 300 r/min, the flowing speed of the syringe pump to be 16 microlitres/min, and the voltage of a high-voltage power supply to be 15 kv.
(3) Starting the electrostatic spinning device, and obtaining the antibacterial and antiviral nano-fiber after 10 hours.
Example 5
A preparation method of antibacterial and antiviral nano-fiber comprises the following steps:
(1) 1.5g of polytetrahydropyrimidine, 1.4g of polyacrylonitrile, and 9g of N, N-dimethylformamide were weighed into a 50mL round-bottomed flask, and stirred at 50 ℃ for 5 hours to obtain a transparent solution.
(2) Adding the transparent solution into a syringe, fixing the syringe on a syringe pump device, enabling the distance between a needle head and a collecting roller device to be 15cm, the rotating speed of the roller to be 250 revolutions per minute, the flowing speed of the syringe pump to be 16 microliters per minute, and the voltage of a high-voltage power supply to be 15 kv.
(3) Starting the electrostatic spinning device, and obtaining the antibacterial and antiviral nano-fiber after 10 hours.
Example 6
A preparation method of antibacterial and antiviral nano-fiber comprises the following steps:
(1) 1.5g of poly (tetrahydropyrimidine) and 2g of polyacrylonitrile and 20g of N, N-dimethylformamide were weighed and added to a 50mL round-bottomed flask, and stirred at 100 ℃ for 3 hours to obtain a transparent solution.
(2) Adding the transparent solution into a syringe, fixing the syringe on a syringe pump device, enabling the distance between a needle head and a collecting roller device to be 15cm, the rotating speed of the roller to be 350 r/min, the flowing speed of the syringe pump to be 20 microliter/min, and the voltage of a high-voltage power supply to be 20 kv.
(3) Starting the electrostatic spinning device, and obtaining the antibacterial and antiviral nano-fiber after 10 hours.
Example 7
A preparation method of antibacterial and antiviral nano-fiber comprises the following steps:
(1) 2g of poly (tetrahydropyrimidine) and 1g of polyacrylonitrile and 5g of N, N-dimethylformamide are weighed and added to a 50mL round-bottom flask, and stirred at 30 ℃ for 10 hours to obtain a transparent solution.
(2) Adding the transparent solution into a syringe, fixing the syringe on a syringe pump device, enabling the distance between a needle head and a collecting roller device to be 15cm, the rotating speed of the roller to be 250 revolutions per minute, the flowing speed of the syringe pump to be 16 microliters per minute, and the voltage of a high-voltage power supply to be 15 kv.
(3) Starting the electrostatic spinning device, and obtaining the antibacterial and antiviral nano-fiber after 10 hours.
Example 8
A preparation method of antibacterial and antiviral nano-fiber comprises the following steps:
(1) 2g of poly (tetrahydropyrimidine), 1.4g of polyacrylonitrile and 9g of N, N-dimethylformamide were weighed and added to a 50mL round-bottomed flask, and stirred at 50 ℃ for 5 hours to obtain a transparent solution.
(2) Adding the transparent solution into a syringe, fixing the syringe on a syringe pump device, enabling the distance between a needle head and a collecting roller device to be 15cm, the rotating speed of the roller to be 250 revolutions per minute, the flowing speed of the syringe pump to be 20 microlitres per minute, and the voltage of a high-voltage power supply to be 20 kv.
(3) Starting the electrostatic spinning device, and obtaining the antibacterial and antiviral nano-fiber after 10 hours.
Example 9
A preparation method of antibacterial and antiviral nano-fiber comprises the following steps:
(1) 2g of poly (tetrahydropyrimidine) and 2g of polyacrylonitrile and 20g of N, N-dimethylformamide were weighed and added to a 50mL round-bottomed flask, and stirred at 100 ℃ for 3 hours to obtain a transparent solution.
(2) Adding the transparent solution into a syringe, fixing the syringe on a syringe pump device, enabling the distance between a needle head and a collecting roller device to be 15cm, the rotating speed of the roller to be 250 revolutions per minute, the flowing speed of the syringe pump to be 16 microliters per minute, and the voltage of a high-voltage power supply to be 15 kv.
(3) Starting the electrostatic spinning device, and obtaining the antibacterial and antiviral nano-fiber after 10 hours.
Characterization of the properties of the antibacterial and antiviral nanofibers prepared in examples 1-9:
(1) the antibacterial performance of the antibacterial and antiviral nanofibers prepared in examples 1 to 9 was tested by colony counting, and the test results showed that the antibacterial and antiviral nanofibers prepared in examples 1 to 3 all had a bacteriostatic ratio of greater than 70% to escherichia coli and staphylococcus aureus, and the antibacterial and antiviral nanofibers prepared in examples 4 to 9 had a bacteriostatic ratio of greater than 99% to escherichia coli and staphylococcus aureus.
(2) The antibacterial and antiviral nanofibers prepared in examples 1 to 9 are cultured with Hela and 3T3 cells, the cell survival rates are all above 85%, and no obvious cell death occurs, and the test results show that the antibacterial and antiviral nanofibers prepared in examples 1 to 9 have good cell compatibility and do not cause harm to human bodies.
(3) The antibacterial and antiviral nanofibers prepared in examples 1 to 9 and the Hela cells added with the lentiviruses are cultured for antiviral performance tests, and the test results show that the antibacterial and antiviral nanofibers prepared in examples 1 to 3 have the inhibition rate of more than 40% on the lentiviruses, and the antibacterial and antiviral nanofibers prepared in examples 4 to 9 have the inhibition rate of more than 50% on the lentiviruses.
Wherein FIG. 2 is a scanning electron microscope test chart of the antibacterial and antiviral nanofibers obtained in example 3; FIG. 3 is a scanning electron microscope test chart of the antibacterial and antiviral nanofibers obtained in example 9; FIG. 4 is a graph showing the inhibitory effect of the antibacterial and antiviral nanofibers obtained in example 3 on Escherichia coli; FIG. 5 is a graph showing the inhibitory effect of the antibacterial and antiviral nanofibers obtained in example 9 on Escherichia coli; FIG. 6 is a graph showing the inhibitory effect of the antibacterial and antiviral nanofibers obtained in example 3 on Staphylococcus aureus; FIG. 7 is a graph showing the inhibitory effect of the antibacterial and antiviral nanofibers obtained in example 9 on Staphylococcus aureus; FIG. 8 is an optical microscope photograph of Hela cells incubated with the antibacterial and antiviral nanofibers obtained in example 3; FIG. 9 is an optical microscope photograph of Hela cells incubated with the antibacterial and antiviral nanofibers obtained in example 9; FIG. 10 is an optical microscope photograph of 3T3 cells incubated with the antibacterial and antiviral nanofibers obtained from example 3; FIG. 11 is an optical microscope photograph of 3T3 cells incubated with the antibacterial and antiviral nanofibers obtained from example 9; FIG. 12 is a fluorescent microscope photograph of the lentivirus cultured with the antibacterial and antiviral nanofibers obtained in example 3; FIG. 13 is a fluorescent microscope photograph of the antibacterial and antiviral nanofiber-cultured lentivirus obtained in example 9.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.

Claims (10)

1. The antibacterial and antiviral nanofiber is characterized by being prepared from the following raw materials:
0.5-2 parts of poly (tetrahydropyrimidine);
1-2 parts of polyacrylonitrile;
5-20 parts of N, N-dimethylformamide.
2. The antibacterial and antiviral nanofiber as claimed in claim 1, wherein the antibacterial and antiviral nanofiber is prepared from the following raw materials:
1.4-1.6 parts by weight of poly (tetrahydropyrimidine);
1.4-1.6 parts of polyacrylonitrile;
7-9 parts of N, N-dimethylformamide.
3. A method for preparing the antibacterial and antiviral nanofiber as claimed in claim 1 or 2, comprising the steps of:
adding the poly-tetrahydropyrimidine and the polyacrylonitrile into N, N-dimethylformamide according to the designed amount, stirring and dissolving to prepare dispersion liquid, and preparing the antibacterial and antiviral nano-fiber from the dispersion liquid by adopting an electrostatic spinning method.
4. The method for preparing antibacterial and antiviral nanofiber as claimed in claim 3, wherein the stirring dissolution is performed by magnetic stirring at 30-100 ℃ for 4-10 hours to obtain a dark brown transparent mixed solution.
5. The method for preparing the antibacterial and antiviral nanofiber as claimed in claim 4, wherein the stirring dissolution is carried out at 50-70 ℃ for 5-8 hours by using magnetic stirring; the voltage of the electrostatic spinning device is 5-20kv, and the flow rate of the injection pump is 5-25 microliter/min.
6. The method for preparing antibacterial and antiviral nanofibers according to claim 5, wherein the distance from the needle of the electrospinning device to the roller is 2-30cm, the rotation speed of the roller is 50-500 rpm, and antibacterial and antiviral nanofibers with good fiber structure and uniform diameter are prepared.
7. The method for preparing antibacterial and antiviral nanofiber as claimed in claim 6, wherein the distance between the electrostatic spinning needle and the collection roller is 10-20cm, and the roller rotation speed is 100-350 rpm.
8. The method for preparing antibacterial and antiviral nanofiber as claimed in claim 7, wherein the dispersion is sucked by a syringe and fixed on a syringe pump device, the flow rate of the syringe pump is 10-20 μ l/min, and the voltage of the high voltage power supply is 10-17 kv.
9. The method for preparing antibacterial and antiviral nanofibers according to claim 8, wherein an electrospinning device is started to obtain antibacterial and antiviral nanofibers with high mechanical strength after 10 hours.
10. Use of the antibacterial and antiviral nanofibers according to claim 1 or 2 in the preparation of protective clothing or masks.
CN202110962752.6A 2021-08-20 2021-08-20 Antibacterial and antiviral nanofiber and preparation method thereof Pending CN113832570A (en)

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CN107619478A (en) * 2017-09-18 2018-01-23 青岛大学 A kind of poly- tetrahydropyrimidine heterocycle antibacterial polymer and preparation method thereof
CN108588887A (en) * 2018-05-04 2018-09-28 东华大学 A kind of antibacterial visualization sub-micron fibers and its preparation and application

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