CN113750173A - 金莲清热制剂在治疗传染性单核细胞增多症中的应用 - Google Patents
金莲清热制剂在治疗传染性单核细胞增多症中的应用 Download PDFInfo
- Publication number
- CN113750173A CN113750173A CN202010505090.5A CN202010505090A CN113750173A CN 113750173 A CN113750173 A CN 113750173A CN 202010505090 A CN202010505090 A CN 202010505090A CN 113750173 A CN113750173 A CN 113750173A
- Authority
- CN
- China
- Prior art keywords
- parts
- preparation
- heat clearing
- flos trollii
- application
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 37
- 241000628997 Flos Species 0.000 title claims abstract description 22
- 201000006747 infectious mononucleosis Diseases 0.000 title claims abstract description 15
- 239000003814 drug Substances 0.000 claims description 17
- 229940079593 drug Drugs 0.000 claims description 15
- 239000007938 effervescent tablet Substances 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 241000612118 Samolus valerandi Species 0.000 claims description 8
- 241001312894 Trollius chinensis Species 0.000 claims description 8
- 229910052602 gypsum Inorganic materials 0.000 claims description 8
- 239000010440 gypsum Substances 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 7
- 239000003826 tablet Substances 0.000 claims description 7
- 241000405414 Rehmannia Species 0.000 claims description 6
- 244000144725 Amygdalus communis Species 0.000 claims description 5
- 235000003893 Prunus dulcis var amara Nutrition 0.000 claims description 5
- 241000334154 Isatis tinctoria Species 0.000 claims description 4
- 241001530121 Trollius Species 0.000 claims description 4
- 239000008187 granular material Substances 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 239000002994 raw material Substances 0.000 claims description 4
- 244000018633 Prunus armeniaca Species 0.000 claims description 3
- 235000009827 Prunus armeniaca Nutrition 0.000 claims description 3
- 239000000706 filtrate Substances 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims description 3
- 238000009472 formulation Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 238000010298 pulverizing process Methods 0.000 claims description 3
- 238000001291 vacuum drying Methods 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 3
- 235000002226 Ranunculus ficaria Nutrition 0.000 claims description 2
- 241000405911 Rehmannia glutinosa Species 0.000 claims description 2
- 239000002775 capsule Substances 0.000 claims description 2
- 239000002552 dosage form Substances 0.000 claims description 2
- 238000002347 injection Methods 0.000 claims description 2
- 239000007924 injection Substances 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims description 2
- 239000006187 pill Substances 0.000 claims description 2
- 239000007921 spray Substances 0.000 claims description 2
- 241000293861 Scrophularia nodosa Species 0.000 claims 1
- 239000006196 drop Substances 0.000 claims 1
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 abstract description 2
- 210000000582 semen Anatomy 0.000 abstract description 2
- 230000005764 inhibitory process Effects 0.000 description 13
- 230000000694 effects Effects 0.000 description 11
- 210000004027 cell Anatomy 0.000 description 9
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 6
- 229960004150 aciclovir Drugs 0.000 description 6
- MKUXAQIIEYXACX-UHFFFAOYSA-N aciclovir Chemical compound N1C(N)=NC(=O)C2=C1N(COCCO)C=N2 MKUXAQIIEYXACX-UHFFFAOYSA-N 0.000 description 6
- 208000024891 symptom Diseases 0.000 description 6
- 206010037660 Pyrexia Diseases 0.000 description 5
- 241000208241 Tropaeolum Species 0.000 description 5
- 239000000427 antigen Substances 0.000 description 5
- 102000036639 antigens Human genes 0.000 description 5
- 108091007433 antigens Proteins 0.000 description 5
- 230000036760 body temperature Effects 0.000 description 5
- 230000004663 cell proliferation Effects 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 239000006228 supernatant Substances 0.000 description 5
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 208000021760 high fever Diseases 0.000 description 4
- 210000004698 lymphocyte Anatomy 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 108010026292 Epstein-Barr viral capsid antigen Proteins 0.000 description 3
- 241000700605 Viruses Species 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- 206010002383 Angina Pectoris Diseases 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 208000010201 Exanthema Diseases 0.000 description 2
- 206010019233 Headaches Diseases 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 239000003443 antiviral agent Substances 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 210000000234 capsid Anatomy 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 201000005884 exanthem Diseases 0.000 description 2
- 229960002963 ganciclovir Drugs 0.000 description 2
- IRSCQMHQWWYFCW-UHFFFAOYSA-N ganciclovir Chemical compound O=C1NC(N)=NC2=C1N=CN2COC(CO)CO IRSCQMHQWWYFCW-UHFFFAOYSA-N 0.000 description 2
- 231100000869 headache Toxicity 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- JXDYKVIHCLTXOP-UHFFFAOYSA-N isatin Chemical compound C1=CC=C2C(=O)C(=O)NC2=C1 JXDYKVIHCLTXOP-UHFFFAOYSA-N 0.000 description 2
- ZXEKIIBDNHEJCQ-UHFFFAOYSA-N isobutanol Chemical compound CC(C)CO ZXEKIIBDNHEJCQ-UHFFFAOYSA-N 0.000 description 2
- 210000005259 peripheral blood Anatomy 0.000 description 2
- 239000011886 peripheral blood Substances 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 206010037844 rash Diseases 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 230000009385 viral infection Effects 0.000 description 2
- 241000205585 Aquilegia canadensis Species 0.000 description 1
- 108010011485 Aspartame Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- GNWUOVJNSFPWDD-XMZRARIVSA-M Cefoxitin sodium Chemical compound [Na+].N([C@]1(OC)C(N2C(=C(COC(N)=O)CS[C@@H]21)C([O-])=O)=O)C(=O)CC1=CC=CS1 GNWUOVJNSFPWDD-XMZRARIVSA-M 0.000 description 1
- 229930186147 Cephalosporin Natural products 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- 241000192125 Firmicutes Species 0.000 description 1
- 208000036066 Hemophagocytic Lymphohistiocytosis Diseases 0.000 description 1
- 208000032672 Histiocytosis haematophagic Diseases 0.000 description 1
- 241000701044 Human gammaherpesvirus 4 Species 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 102000014150 Interferons Human genes 0.000 description 1
- 108010050904 Interferons Proteins 0.000 description 1
- 206010025323 Lymphomas Diseases 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 208000002454 Nasopharyngeal Carcinoma Diseases 0.000 description 1
- 206010061306 Nasopharyngeal cancer Diseases 0.000 description 1
- 240000002853 Nelumbo nucifera Species 0.000 description 1
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 1
- 206010068319 Oropharyngeal pain Diseases 0.000 description 1
- 201000007100 Pharyngitis Diseases 0.000 description 1
- 241001377612 Polygonum glaucescens Species 0.000 description 1
- 239000012980 RPMI-1640 medium Substances 0.000 description 1
- 244000081426 Ranunculus ficaria Species 0.000 description 1
- 206010039424 Salivary hypersecretion Diseases 0.000 description 1
- 241000191940 Staphylococcus Species 0.000 description 1
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 229940124977 antiviral medication Drugs 0.000 description 1
- 235000010357 aspartame Nutrition 0.000 description 1
- 239000000605 aspartame Substances 0.000 description 1
- IAOZJIPTCAWIRG-QWRGUYRKSA-N aspartame Chemical compound OC(=O)C[C@H](N)C(=O)N[C@H](C(=O)OC)CC1=CC=CC=C1 IAOZJIPTCAWIRG-QWRGUYRKSA-N 0.000 description 1
- 229960003438 aspartame Drugs 0.000 description 1
- 229960004099 azithromycin Drugs 0.000 description 1
- MQTOSJVFKKJCRP-BICOPXKESA-N azithromycin Chemical compound O([C@@H]1[C@@H](C)C(=O)O[C@@H]([C@@]([C@H](O)[C@@H](C)N(C)C[C@H](C)C[C@@](C)(O)[C@H](O[C@H]2[C@@H]([C@H](C[C@@H](C)O2)N(C)C)O)[C@H]1C)(C)O)CC)[C@H]1C[C@@](C)(OC)[C@@H](O)[C@H](C)O1 MQTOSJVFKKJCRP-BICOPXKESA-N 0.000 description 1
- SIKJAQJRHWYJAI-UHFFFAOYSA-N benzopyrrole Natural products C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 description 1
- 229960003016 cefoxitin sodium Drugs 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 229940124587 cephalosporin Drugs 0.000 description 1
- 150000001780 cephalosporins Chemical class 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000000071 effect on fever Effects 0.000 description 1
- BJDWURMSYDDCRX-UHFFFAOYSA-N ethoxycarbonyl ethyl carbonate;hydrate Chemical compound O.CCOC(=O)OC(=O)OCC BJDWURMSYDDCRX-UHFFFAOYSA-N 0.000 description 1
- 239000012091 fetal bovine serum Substances 0.000 description 1
- 229930182486 flavonoid glycoside Natural products 0.000 description 1
- 150000007955 flavonoid glycosides Chemical class 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 208000014752 hemophagocytic syndrome Diseases 0.000 description 1
- 206010019847 hepatosplenomegaly Diseases 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 1
- -1 indole glycosides Chemical class 0.000 description 1
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 229940079322 interferon Drugs 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000001990 intravenous administration Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 230000005976 liver dysfunction Effects 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 241001515942 marmosets Species 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000003680 myocardial damage Effects 0.000 description 1
- 201000011216 nasopharynx carcinoma Diseases 0.000 description 1
- 230000003285 pharmacodynamic effect Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 208000026451 salivation Diseases 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 235000002906 tartaric acid Nutrition 0.000 description 1
- 239000011975 tartaric acid Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/06—Aluminium, calcium or magnesium; Compounds thereof, e.g. clay
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/19—Acanthaceae (Acanthus family)
- A61K36/195—Strobilanthes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
- A61K36/315—Isatis, e.g. Dyer's woad
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
- A61K36/704—Polygonum, e.g. knotweed
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/736—Prunus, e.g. plum, cherry, peach, apricot or almond
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/80—Scrophulariaceae (Figwort family)
- A61K36/804—Rehmannia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/80—Scrophulariaceae (Figwort family)
- A61K36/808—Scrophularia (figwort)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/85—Verbenaceae (Verbena family)
- A61K36/855—Clerodendrum, e.g. glorybower
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
- A61K36/8964—Anemarrhena
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/20—Antivirals for DNA viruses
- A61P31/22—Antivirals for DNA viruses for herpes viruses
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Epidemiology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Alternative & Traditional Medicine (AREA)
- Mycology (AREA)
- Virology (AREA)
- Molecular Biology (AREA)
- Inorganic Chemistry (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
本发明涉及金莲清热制剂在治疗传染性单核细胞增多症中的应用。所述金莲清热制剂是由金莲花、大青叶、石膏、知母、地黄、玄参、炒苦杏仁制备而成。临床研究表明,金莲清热制剂能够有效治疗传染性单核细胞增多症。
Description
技术领域
本发明涉及药物应用技术领域,尤其是金莲清热制剂在治疗传染性单核细胞增多症中的应用。
背景技术
传染性单核细胞增多症(infectious mononucleosis,IM),俗称“传单”,是由EB病毒(Epstein-Barr virus,E-BV)所致的急性感染性疾病,主要侵犯儿童和青少年,临床表现复杂多样,症状轻重不一,主要表现为不规则发热,无固定热型,咽峡炎、***肿大、皮疹、外周血中淋巴细胞增高及异常淋巴细胞增高超过10%。传染性单核细胞增多症文献显示,该症患者容易引起心肌损害和肝功能异常,少部分容易诱发慢性活动性EB病毒感染、鼻咽癌、恶性淋巴瘤及EBV相关噬血细胞性淋巴组织细胞增生症等,必须予以重视。目前该病没有特别有效的药物治疗,临床上针以此病症主要使用抗病毒药(阿昔洛韦、更昔洛韦等)及抗生素(头孢、阿奇霉素等)治疗, 但此类药副作用大,疗效一般。
金莲清热制剂由金莲花、大青叶、石膏、知母、地黄、玄参、炒苦杏仁制备而成,具有清热解毒,生津利咽,止咳祛痰的功效。现代药理研究证实,金莲花属于广谱抑菌药,其主要成份黄酮苷对革兰氏阳性菌、革兰氏阴性菌及葡萄球菌等都具有较好的抑制作用,大青叶中的大青素B、蓼蓝叶中的吲哚苷体外显示有抗病毒作用,但金莲清热制剂在治疗传染性单核细胞增多症的作用还未见报道。
发明内容
本发明的目的在于提供金莲清热制剂在治疗传染性单核细胞增多症中的应用。
本发明所述金莲清热制剂是由金莲花、大青叶、石膏、知母、地黄、玄参、炒苦杏仁按一定制备工艺制备而成,其制备方法属于现有技术,可以从现有专利公开的文件或国家药品标准中获得。如CN200510098725.X、YBZ11572006-2009Z。
本发明所述金莲清热制剂是由如下重量份比例的原料药制成: 1400-1600份金莲花、1400-1600份大青叶、1100-1200份石膏、700-800份知母、700-800份地黄、700-800份玄参、1100-1200份炒苦杏仁。
具体的,所述金莲清热制剂是由如下重量份比例的原料药制成:1500份金莲花、1500份大青叶、1125份石膏、750份知母、750份地黄、750份玄参、1125份炒苦杏仁。
所述金莲清热制剂的制备方法为:按上述配比称取金莲花、大青叶、石膏、知母、地黄、玄参、炒苦杏仁混合,加水煎煮二次,第一次加7~8倍量水,煎煮2小时;第二次 加5~6倍量水,煎煮1小时,合并煎液,滤过,滤液减压浓缩至相对密度50℃热测为1.05~1.40的清膏,真空干燥,粉碎成细粉,再根据需要加入一种或多种药学上可接受的辅料,制成药学上可接受的剂型。
所述金莲清热制剂的剂型为胶囊剂、颗粒剂、片剂(含泡腾片)、液体制剂、滴丸剂、喷雾剂、直肠给药制剂、注射剂。优选为泡腾片。
本发明所述的金莲清热制剂在使用时根据病人的情况确定用法用量。如已上市的天津中盛海天生产的金莲清热泡腾片,成人一次2片,一日4次,高烧时每4小时服1次,小儿1岁以下每次1片,一日3次,高烧时每日4次;又如宁夏启元生产的金莲清热颗粒,成人一次5克,一日4次,高烧时每4小时服1次,小儿1岁以下每次2.5克,一日3次,高烧时每日4次。
传染性单核细胞增多症目前临床发病症状为:主要发病为儿童和青少年,临床表现复杂多样,症状轻重不一,主要表现发热,咽痛、***肿大、肝/脾肿大、皮疹、外周血中淋巴细胞增高等。
为了进一步探究金莲清热制剂在治疗传染性单核细胞增多症药物中的应用,本发明进行了体外药效学及临床用药研究。结果显示,本发明所述的金莲清热制剂具有较好的治疗传染性单核细胞增多症的作用。具体地说,本发明提供的金莲清热制剂对EB病毒所致的发热、咽痛、***肿大等的疗效确切、安全性好、恢复程度快,值得推广。
具体实施方式:
下面结合具体的实施方式来对本发明的技术方案做进一步的限定,但要求保护的范围不仅局限于所作的描述。
实验一:金莲清热制剂对B95-8内衣壳抗原(VCA)的影响
1、实验材料:B95-8是绒猴EBV转化的淋巴细胞,能释放EBV的连续细胞株,由含10%胎牛血清的RPMI-1640培养。
2、实验方法:
2.1细胞增殖抑制试验:将处于对数生长期的B95-8细胞离心后,培养于96孔板中,培养基含有不同浓度的金莲清热制剂或阿昔洛韦,金莲清热制剂设5个剂量组为:200、40、8、1.6、0.32mg/ml,为金莲清热泡腾片加水泡腾溶解后稀释所得,阿昔洛韦设5个剂量组为:40、8、1.6、0.32、0.064 mg/ml。于37℃继续教育培养48小时,离心弃上清液,加入新鲜不含药物培养基,再加10μlMTT溶液(5mg/ml), 37℃孵育4小时,加入100μl三联液(取SDS10g,异丁醇5ml,10MHCl0.1ml,加100ml重蒸水溶解),使结晶溶解。以溶剂处理的细胞为对照组,酶标仪测定吸光度(A),测定波长为570nm。细胞的增殖抑制率=(对照组A值-药物组A值)/对照组A值×100%。
2.2对B95-8内衣壳抗原(VCA)的影响:将金莲清热泡腾片加20ml水泡腾溶解后,采用MTT实验方法选择药物浓度对细胞增殖抑制率低于10%为大剂量,等级稀释分为大、中、小三个剂量,另设正常对照组、模型组、阳性对照组(阿昔洛韦),每组各三个样,除正常对照组外,其余各组均采用丁酸和TAP诱导细胞。药物作用48h后,将B95-8细胞离心,弃上清液,加入1ml TRlzol Reagent,混合样品至重悬。离心吸取上清液,加入0.2ml三氯甲烷,离心后加入0.5ml异丙醇,离心弃上清液,75%乙醇洗涤沉淀,离心弃上清液,短暂干燥RNA沉淀。用焦碳酸二乙酯水15μl溶解沉淀,-80℃保存。
3、实验结果:
3.1细胞增殖抑制试验:不同浓度的金莲清热制剂和阿昔洛韦分别作用于B95-8细胞48h,检测增殖抑制率。结果显示两种药物对B95-8细胞的生长抑制作用随着药物浓度的升高,抑制率也升高。结果见表1、表2:
表1不同浓度金莲清热制剂对B95-8细胞增殖抑制作用结果
浓度(mg/ml) | 200 | 40 | 8 | 1.6 | 0.32 |
抑制率(%) | 92.5 | 66.3 | 28.4 | 18.4 | 6.2 |
表2不同浓度阿昔洛韦对B95-8细胞增殖抑制作用结果
浓度(mg/ml) | 40 | 8 | 1.6 | 0.32 | 0.064 |
抑制率(%) | 98.4 | 72.8 | 38.1 | 14.8 | 1.52 |
3.2对B95-8内衣壳抗原(VCA)的影响:采用丁酸和TAP诱导B95-8细胞后,EBV衣壳抗原分泌明显增多,给药组EBV衣壳抗原分泌明显降低,其中金莲清热大、中剂量组均能显著抑制丁酸和TAP诱导B95-8细胞分泌EBV衣壳抗原,小剂量组对其有抑制作用,结果见表3:
表3金莲清热泡腾片对B95-8细胞内衣壳抗原(VCA)的影响
组别 | 剂量(mg/ml) | 病毒载量(cp/ml) | 抑制率(%) |
正常对照组 | ―― | 175.32±24.62 | ―― |
模型对照组 | ―― | 2187.54±65.27 | ―― |
阳性对照组 | 0.32 | 215.31±32.33 | 90.16 |
金莲清热大剂量组 | 1.6 | 217.64±48.52 | 90.05 |
金莲清热中剂量组 | 0.8 | 219.61±35.24 | 89.96 |
金莲清热小剂量组 | 0.4 | 905.47±48.21 | 58.61 |
实验二:临床经典病例:
1、李XX,女,5岁,发热3天、头痛、咽痛,EB病毒衣壳抗原IgM、IgG抗体阳性;诊断为EB病毒感染,入院后给予抗病毒等静脉点滴药物治疗后体温仍反复,于入院第九天改为口服金莲清热泡腾片治疗2天(每次1.5片,每日4次),体温下降至正常且平稳,一周后临床症状基本消失且恢复正常。
2、赵XX,男,6岁,发热6天, 3天前出现发热,体温37.6℃,伴随咽痛,家中口服抗病毒药物,效果不佳,后经检查确诊为传染性单核细胞增多症,给予头孢西丁钠、更昔洛韦及干扰素治疗后体温反复,口服金莲清热泡腾片2天后(每次2片,每日4次),体温恢复正常,4天后头痛及呼吸道症状消失,一周后所有临床症状消失并恢复正常。
实验三:金莲清热泡腾片的制备
称取金莲花1500g、大青叶1500g、石膏1125g、知母750g、地黄750g、玄参750g、炒苦杏仁1125g混合,加水煎煮二次,第一次加7~8倍量水,煎煮2小时;第二次 加5~6倍量水,煎煮1小时,合并煎液,滤过,滤液减压浓缩至相对密度50℃热测为1.25的清膏,真空干燥,粉碎成细粉,加入酒石酸1200g、阿司帕坦40g及甘露醇适量,混匀、制粒、干燥,压制成1000片,即得。
在此有必要指出的是,以上实施方式仅限于对本发明的技术方案做进一步的阐述和理解,不能理解为对本发明的技术方案做进一步的限定,本领域技术人员作出的非突出实质性特征和显著进步的发明创造,仍然属于本发明的保护范畴。
Claims (6)
1.金莲清热制剂在制备治疗传染性单核细胞增多症药物中的应用。
2.如权利要求1所述的应用,其特征在于,所述金莲清热制剂由如下重量份比例的原料药制成: 1400-1600份金莲花、1400-1600份大青叶、1100-1200份石膏、700-800份知母、700-800份地黄、700-800份玄参、1100-1200份炒苦杏仁。
3.如权利要求1所述的应用,其特征在于,所述金莲清热制剂由如下重量份比例的原料药制成:1500份金莲花、1500份大青叶、1125份石膏、750份知母、750份地黄、750份玄参、1125份炒苦杏仁。
4.如权利要求1-3任一项所述的应用,其特征在于,所述金莲清热制剂的制备方法为:按配比称取金莲花、大青叶、石膏、知母、地黄、玄参、炒苦杏仁混合,加水煎煮二次,第一次加7~8倍量水,煎煮2小时;第二次 加5~6倍量水,煎煮1小时,合并煎液,滤过,滤液减压浓缩至相对密度50℃热测为1.05~1.40的清膏,真空干燥,粉碎成细粉,再根据需要加入一种或多种药学上可接受的辅料,制成药学上可接受的剂型。
5.如权利要求4所述的应用,所述金莲清热制剂的剂型为胶囊剂、颗粒剂、片剂、泡腾片、液体制剂、滴丸剂、喷雾剂、直肠给药制剂、注射剂。
6.如权利要求5所述的应用,其特征在于,所述金莲清热制剂的剂型为泡腾片。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010505090.5A CN113750173A (zh) | 2020-06-05 | 2020-06-05 | 金莲清热制剂在治疗传染性单核细胞增多症中的应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010505090.5A CN113750173A (zh) | 2020-06-05 | 2020-06-05 | 金莲清热制剂在治疗传染性单核细胞增多症中的应用 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN113750173A true CN113750173A (zh) | 2021-12-07 |
Family
ID=78784943
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202010505090.5A Pending CN113750173A (zh) | 2020-06-05 | 2020-06-05 | 金莲清热制剂在治疗传染性单核细胞增多症中的应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN113750173A (zh) |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1460513A (zh) * | 2003-06-11 | 2003-12-10 | 宁夏启元药业有限公司 | 金莲清热胶囊及其制备工艺 |
CN1733246A (zh) * | 2005-09-07 | 2006-02-15 | 北京因科瑞斯生物制品研究所 | 一种金莲清热泡腾片及其制备方法 |
CN107007566A (zh) * | 2016-01-27 | 2017-08-04 | 天津中盛海天制药有限公司 | 金莲清热泡腾片新生产工艺 |
-
2020
- 2020-06-05 CN CN202010505090.5A patent/CN113750173A/zh active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1460513A (zh) * | 2003-06-11 | 2003-12-10 | 宁夏启元药业有限公司 | 金莲清热胶囊及其制备工艺 |
CN1733246A (zh) * | 2005-09-07 | 2006-02-15 | 北京因科瑞斯生物制品研究所 | 一种金莲清热泡腾片及其制备方法 |
CN107007566A (zh) * | 2016-01-27 | 2017-08-04 | 天津中盛海天制药有限公司 | 金莲清热泡腾片新生产工艺 |
Non-Patent Citations (3)
Title |
---|
于祥等: "金莲清热泡腾片治疗小儿疱疹性口腔炎61例疗效观察", 《中国中西医结合儿科学》 * |
李建东等: "金莲清热颗粒治疗小儿上呼吸道感染疗效观察", 《中国社区医师》 * |
肖国士等: "《中医临床实习手册》", 31 August 2000 * |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN114053343B (zh) | 一种中药组合物、制备方法及应用 | |
CN106138139B (zh) | 人参提取物、人参皂苷、人参皂苷衍生物在制备治疗巨细胞病毒感染病症的药物中的应用 | |
CN108524685B (zh) | 一种治疗儿童疱疹性咽峡炎的组合物及其应用 | |
CN103006773B (zh) | 一种小儿清热宁中药颗粒 | |
CN111700998A (zh) | 复方中成药在治疗新型冠状病毒感染的肺炎covid-19中的应用 | |
CN113750173A (zh) | 金莲清热制剂在治疗传染性单核细胞增多症中的应用 | |
JP2005502602A (ja) | ウイルス感染の治療用の医薬組成物 | |
CN101683423B (zh) | 一种中药组合物在制备治疗水痘药物中的应用 | |
CN101134092A (zh) | 一种治疗呼吸道疾病的中药复方制剂及其制备方法 | |
EP4209225A1 (en) | Forsythiae fructus component and optional ginseng component, and use thereof | |
CN101698005B (zh) | 一种用于治疗感冒、流感的黄金颗粒及其制备方法 | |
CN110548076A (zh) | 一种中药组合物在制备治疗或预防湿疹药物中的应用 | |
CN100475202C (zh) | 具有清热解毒作用的药物组合物 | |
CN117942376B (zh) | 一种组合物在制备提高免疫力药物中的应用 | |
CN103211878A (zh) | 一种抗感染的中药组合物及其制备方法 | |
CN114984108B (zh) | 一种治疗银屑病的中药组合物及其制备方法 | |
CN115607587B (zh) | 艾迪制剂在制备治疗带状疱疹药物中的应用 | |
CN101934048B (zh) | 一种治疗呼吸道感染性疾病的中药复方制剂及其制备方法 | |
CN111939263B (zh) | 药物组合物、中药组合物及其提取物和制备、药剂、用途 | |
CN108354957B (zh) | 马兰或马兰提取物在制备治疗非感染性炎症的药物中的应用及药物 | |
CN105079135A (zh) | 一种治疗感冒的中药组合物及其制备方法 | |
CN114053326A (zh) | 珍珠滴丸在制备治疗小儿手足口症药物中的应用 | |
CN114366771A (zh) | 一种防治高尿酸血症的中药组合物、制剂、方法及应用 | |
CN104888123A (zh) | 一种用于小儿呼吸道感染的制剂及其制备和检测方法 | |
CN113648357A (zh) | 一种中药组合物在制备抗炎药物和/或免疫调节药物中的应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
RJ01 | Rejection of invention patent application after publication | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20211207 |