CN113383851B - Method for extracting peony isolated protein for oil - Google Patents
Method for extracting peony isolated protein for oil Download PDFInfo
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- CN113383851B CN113383851B CN202110752411.6A CN202110752411A CN113383851B CN 113383851 B CN113383851 B CN 113383851B CN 202110752411 A CN202110752411 A CN 202110752411A CN 113383851 B CN113383851 B CN 113383851B
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- 241000736199 Paeonia Species 0.000 title claims abstract description 80
- 235000006484 Paeonia officinalis Nutrition 0.000 title claims abstract description 80
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 65
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 65
- 238000000034 method Methods 0.000 title claims abstract description 31
- 238000000605 extraction Methods 0.000 claims abstract description 51
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 40
- 235000012054 meals Nutrition 0.000 claims abstract description 39
- 239000000284 extract Substances 0.000 claims abstract description 24
- 238000003916 acid precipitation Methods 0.000 claims abstract description 18
- 239000006228 supernatant Substances 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000001273 butane Substances 0.000 claims abstract description 9
- IJDNQMDRQITEOD-UHFFFAOYSA-N n-butane Chemical compound CCCC IJDNQMDRQITEOD-UHFFFAOYSA-N 0.000 claims abstract description 9
- OFBQJSOFQDEBGM-UHFFFAOYSA-N n-pentane Natural products CCCCC OFBQJSOFQDEBGM-UHFFFAOYSA-N 0.000 claims abstract description 9
- 238000005406 washing Methods 0.000 claims abstract description 9
- 238000001694 spray drying Methods 0.000 claims abstract description 7
- 230000007935 neutral effect Effects 0.000 claims abstract description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 21
- 238000005238 degreasing Methods 0.000 claims description 17
- 239000007788 liquid Substances 0.000 claims description 13
- 239000002904 solvent Substances 0.000 claims description 6
- 239000003795 chemical substances by application Substances 0.000 claims description 4
- 239000003513 alkali Substances 0.000 abstract description 19
- 230000008569 process Effects 0.000 abstract description 10
- 101001018064 Homo sapiens Lysosomal-trafficking regulator Proteins 0.000 abstract 1
- 102100033472 Lysosomal-trafficking regulator Human genes 0.000 abstract 1
- 235000010703 Modiola caroliniana Nutrition 0.000 abstract 1
- 244000038561 Modiola caroliniana Species 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 description 55
- 235000019198 oils Nutrition 0.000 description 44
- 239000000243 solution Substances 0.000 description 8
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 7
- 229930003944 flavone Natural products 0.000 description 7
- 150000002212 flavone derivatives Chemical class 0.000 description 7
- 235000011949 flavones Nutrition 0.000 description 7
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 7
- 238000000751 protein extraction Methods 0.000 description 6
- 239000000469 ethanolic extract Substances 0.000 description 5
- YKRGDOXKVOZESV-WRJNSLSBSA-N Paeoniflorin Chemical compound C([C@]12[C@H]3O[C@]4(O)C[C@](O3)([C@]1(C[C@@H]42)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)C)OC(=O)C1=CC=CC=C1 YKRGDOXKVOZESV-WRJNSLSBSA-N 0.000 description 4
- 230000001804 emulsifying effect Effects 0.000 description 4
- 238000005457 optimization Methods 0.000 description 4
- YKRGDOXKVOZESV-UHFFFAOYSA-N paeoniflorin Natural products O1C(C)(C2(CC34)OC5C(C(O)C(O)C(CO)O5)O)CC3(O)OC1C24COC(=O)C1=CC=CC=C1 YKRGDOXKVOZESV-UHFFFAOYSA-N 0.000 description 4
- 238000000194 supercritical-fluid extraction Methods 0.000 description 4
- 235000010469 Glycine max Nutrition 0.000 description 3
- 244000068988 Glycine max Species 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 108010073771 Soybean Proteins Proteins 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 239000006260 foam Substances 0.000 description 3
- 238000002386 leaching Methods 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 description 2
- 241000896531 Paeonia rockii Species 0.000 description 2
- 108010064851 Plant Proteins Proteins 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 235000021118 plant-derived protein Nutrition 0.000 description 2
- 239000012460 protein solution Substances 0.000 description 2
- 238000004537 pulping Methods 0.000 description 2
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 description 2
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 2
- 235000005493 rutin Nutrition 0.000 description 2
- 229960004555 rutoside Drugs 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 229940001941 soy protein Drugs 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 235000015112 vegetable and seed oil Nutrition 0.000 description 2
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 1
- 241000847925 Paeonia ostii Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 230000003908 liver function Effects 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000010979 pH adjustment Methods 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000001814 protein method Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000019710 soybean protein Nutrition 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- SWGJCIMEBVHMTA-UHFFFAOYSA-K trisodium;6-oxido-4-sulfo-5-[(4-sulfonatonaphthalen-1-yl)diazenyl]naphthalene-2-sulfonate Chemical group [Na+].[Na+].[Na+].C1=CC=C2C(N=NC3=C4C(=CC(=CC4=CC=C3O)S([O-])(=O)=O)S([O-])(=O)=O)=CC=C(S([O-])(=O)=O)C2=C1 SWGJCIMEBVHMTA-UHFFFAOYSA-K 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
- A23J1/142—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds by extracting with organic solvents
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/14—Vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Peptides Or Proteins (AREA)
Abstract
A method for extracting peony protein isolate for oil comprises the following steps: step one: shelling the oil peony seeds, pressing cakes or crushing the peony seeds (hydraulic) and granulating, extracting butane for 1-4 times at low temperature to obtain defatted oil peony seeds; step two: alcohol-extracting the peony seed meal obtained in the step one for 1-3 times to obtain peony seed meal from which the alcohol extract is removed; step three: and (3) carrying out alkali extraction on the peony seed meal with the alcohol extract removed, which is obtained in the step (II), carrying out acid precipitation on the alkali extraction supernatant under the conditions of pH3-5 and time 1-4 hours, centrifuging, washing with water to be neutral, and carrying out spray drying to obtain the peony protein isolate for oil. The application adopts butane low-temperature extraction, ensures that the protein is not denatured under the action of high temperature, reduces the content of oil and fat in the meal to the minimum and improves the purity of the protein; the application utilizes alcohol extraction to treat peony meal, the protein obtained by alkali extraction and acid precipitation is light yellow after the step, and the color is mauve if the step is not performed; the extraction rate and purity of the protein reach the highest under the alkaline extraction and acid precipitation process, and the extraction rate is 51.62% and the purity is 94%.
Description
Technical Field
The application belongs to the research field of peony isolated proteins, and particularly relates to a method for extracting oil-used peony isolated proteins.
Background
The peony for oil is mainly paeonia ostii and paeonia rockii, has the characteristics of large seed yield, high oil content, excellent quality, strong resistance and the like, and generates 60% -70% of peony cake after oil is squeezed from the paeonia rockii seeds, and contains rich nutrients such as protein, polysaccharide, flavone and the like, wherein the protein content accounts for about 20% -33% of the weight of the cake, the amino acid proportion is balanced, and the peony isolated protein has the functional characteristics of good water absorption, oil holding property, emulsifying stability, foamability, foam stability and the like, and in addition, the peony isolated protein does not contain cholesterol, is more easily digested and absorbed by human bodies, has a protective effect on liver functions, is beneficial to preventing cardiovascular diseases and is a plant protein with great commercial development value.
The process of alkali extraction and acid precipitation for industrial production of vegetable protein, soybean protein as example, is as follows: soybean, squeezing, degreasing cake, alkali extraction, centrifugal separation, pH adjustment of supernatant to isoelectric point, centrifugal separation, water washing of precipitate to neutrality, drying and protein. Alkali extraction and acid precipitation are the only ways to extract plant protein isolate, but the process method is different, and the purity, color, physicochemical properties and the like of the protein isolate prepared by extraction are very different, which directly determines the quality of the protein isolate. At present, few reports on the extraction of peony isolated proteins for oil are provided, and the existing method has the problems of low quality of the extracted isolated proteins, difficulty in large-scale industrialization and the like.
For example, CN108887461a discloses "peony seed protein and extraction method thereof", the steps are: separating the shell from the kernel after shelling the peony seeds, crushing and preheating the kernel, separating the peony seed oil by a supercritical extraction method, obtaining the peony seed meal as the rest, drying and crushing the separated peony seed meal powder, adding distilled water with a certain mass ratio, homogenizing at a high speed, adding NaOH solution to adjust to a proper pH value, stirring and leaching for a certain time at 40-60 ℃, centrifuging the leaching solution, collecting supernatant to obtain a peony protein solution, then adjusting the pH value of the peony protein solution to be acidic by an HCl solution, obtaining precipitated peony protein, centrifuging and washing to be neutral, and drying to obtain the peony seed protein. The method comprises the steps of separating the shell from the kernel, performing preheating treatment on the kernel, and then separating the peony seed oil by using a supercritical extraction method, wherein the preheating treatment is easy to generate local high temperature in large-scale implementation, so that protein denaturation is caused; in addition, the supercritical extraction throughput is limited, the throughput per batch is not more than 200kg, the production capacity is extremely limited, and the mass production cannot be realized.
CN110226664a discloses a preparation method of peony seed protein powder, which comprises the following steps: taking defatted peony seed cake meal as a raw material, pulping by alkaline oxidation potential water, and vacuumizing and carrying out negative pressure soaking; ultrasonic low-temperature extraction; centrifugally separating the protein extract from the peony seed residues; regulating pH value of supernatant to near isoelectric point of protein with hydrochloric acid; centrifuging to separate out protein; washing with water, neutralizing and pulping; and (5) spray drying to obtain the peony protein powder product. The method adopts alkaline oxidation potential water pulp mixing and still adopts an alkaline protein extraction mode, and is characterized in that the method is used for vacuumizing and negative pressure soaking, and because peony isolated protein is dissolved under alkaline conditions, whether negative pressure is used has no direct influence on the protein extraction rate, and other impurities are mixed in the peony isolated protein when seed meal is extracted under negative pressure, and other impurities are adsorbed by the separated isolated protein during an acid precipitation process, so that the obtained isolated protein has low extraction rate and poor color and luster, and the requirements of markets on high-quality isolated protein are difficult to meet.
Disclosure of Invention
The application provides a method for extracting peony protein isolate for oil, which is used for solving the defects in the prior art.
The application is realized by the following technical scheme:
a method for extracting peony isolated protein for oil comprises the following steps:
step one: the oil peony seed is dehulled, pressed into cakes or subjected to hydraulic pressure, crushed and granulated, butane is used as an extractant, and the oil peony seed meal for degreasing with the oil content less than or equal to 1.5% is obtained by extraction for 1-4 times under the conditions that the pressure is 0.3mpa-0.8mpa and the extraction temperature is 20-50 ℃;
step two: and (3) taking methanol or ethanol as a solvent for the peony seed meal for the degreasing oil obtained in the step one, wherein the alcohol concentration is 50% -95%, and the feed liquid ratio is 1:5-1:15, extracting for 1-3 times at the extraction temperature of 40-50 ℃ for 1-3 hours to obtain the peony seed meal for degreasing oil with the alcohol extract removed;
step three: the defatted oil obtained in the second step and the alcohol extract removed is subjected to peony seed meal at the temperature of 30-50 ℃ and the feed-liquid ratio of 1:15-1:25, pH10-11 (0.005 mol/L-0.01 mol/L), alkali extraction under the condition of 2-5 h, centrifuging to obtain alkali extraction supernatant, acid precipitation of the alkali extraction supernatant under the condition of pH3-5 and 1-4 h, centrifuging, washing the obtained protein to neutrality, and spray drying to obtain the peony protein isolate for oil.
The peony seed meal in the first step is the peony seed meal for oil, which is dehulled, pressed into cakes or subjected to hydraulic pressure.
The method for extracting the peony isolated protein for oil comprises the step two, wherein the alcohol extract obtained in the step two volatilizes the solvent and contains flavone and paeoniflorin.
The application has the advantages that:
(1) The application adopts butane to extract peony seeds or peony seed meal (hydraulic pressure) at low temperature, firstly, the disposable treatment capacity is large, the cost is low, the large-scale production can be realized, and the method is far superior to the supercritical extraction method; secondly, the low-temperature extraction is ensured, so that the protein property is intact and is not destroyed, and the double-screw squeezing method generates high temperature in the squeezing process, so that the protein is denatured, the physical and chemical properties, biochemical properties and the like of the protein are greatly affected, and the hydraulic method has low temperature, but the content of the obtained defatted seed meal residual oil is still high, so that the residual oil still needs to be removed through butane low-temperature extraction.
(2) The application utilizes methanol or ethanol to treat peony seed meal, the color of the obtained alcohol extract is as shown in figure 2, the color of the alcohol extract is brown yellow, and the components such as flavone, paeoniflorin and the like are primarily analyzed. The protein color after alkali extraction and acid precipitation is light yellow, if the acid precipitation is not performed by the alkali extraction and acid precipitation, the obtained protein color is purple red (see figure 3), the step is a key step of removing the protein color discovered for the first time, and the obtained alcohol extract contains active ingredients such as flavone and paeoniflorin, and can be purified and used in other fields such as cosmetics, so that the full utilization of resources is realized.
(3) At the alkaline extraction temperature of 30-50 ℃, the alkaline extraction time of 2-5 h, and the alkaline extraction liquid ratio of 1:15-1:25, the extraction rate and purity of the protein can be highest under the process conditions of alkaline extraction pH10-11 (0.005 mol/L-0.01 mol/L), acid precipitation time 1h-4h and acid precipitation pH3-5, the extraction rate is 51.62%, the purity is 94%, and the functional properties of protein water absorption, oil holding, emulsifying property, emulsifying stability, foamability, foam stability and the like are superior to those of the isolated soy protein.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions of the prior art, the drawings that are needed in the embodiments or the description of the prior art will be briefly described below, it will be obvious that the drawings in the following description are some embodiments of the present application, and that other drawings can be obtained according to these drawings without inventive effort to a person skilled in the art.
FIG. 1 is a process flow of extracting the peony isolated protein for oil;
FIG. 2 is a photograph of an alcohol extract obtained according to the present application;
the left graph in fig. 3 shows the peony protein isolate for oil obtained by direct alkaline extraction and acid precipitation without leaching with methanol or ethanol; the right graph shows the peony isolated protein for oil obtained by the application;
FIG. 4 is an acid precipitation time optimization result of the present application;
FIG. 5 shows the results of pH optimization for acid precipitation in accordance with the present application.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present application more apparent, the technical solutions of the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application, and it is apparent that the described embodiments are some embodiments of the present application, but not all embodiments of the present application. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
Example 1:
step one: crushing and granulating the peony seed meal subjected to hydraulic pressure, and extracting for 2 times under the condition that the pressure is 0.4mpa and the extraction temperature is 40 ℃ by taking butane as an extracting agent to obtain the peony seed meal for degreasing oil with the oil content of 1.3%;
step two: and (3) taking ethanol as a solvent for the peony seed meal for degreasing oil obtained in the step one, wherein the alcohol concentration is 70%, and the feed liquid ratio is 1:5, extracting for 2 times at the extraction temperature of 40 ℃ for 1h to obtain the peony seed meal for degreasing oil with the alcohol extract removed, wherein the yield of the alcohol extract is 10%;
step three: the degreasing oil of the ethanol extract obtained in the second step is subjected to peony seed meal at the temperature of 40 ℃ and the feed-liquid ratio of 1:15, pH10 (0.005 mol/L), alkali extraction under the condition of 2.5h, centrifuging to obtain alkali extraction supernatant, acid precipitation of the alkali extraction supernatant under the condition of pH3 and 2h, centrifuging, washing the obtained protein with water to neutrality, and spray drying to obtain oil peony protein isolate with a protein extraction rate of 41.2% and a purity of 91%.
Example 2:
step one: crushing and granulating the peony seed meal subjected to hydraulic pressure, and extracting for 4 times under the condition that the pressure is 0.48mpa and the extraction temperature is 50 ℃ by taking butane as an extracting agent to obtain the peony seed meal for degreasing oil with the oil content of 0.94%;
step two: and (3) taking methanol as a solvent for the peony seed meal for degreasing oil obtained in the step one, wherein the alcohol concentration is 90%, and the feed liquid ratio is 1:10, extracting for 3 times at 45 ℃ for 3 hours to obtain defatted oil peony seed meal from which the alcohol extract is removed, wherein the yield of the alcohol extract is 14%;
step three: the degreasing oil of the ethanol extract obtained in the second step is subjected to peony seed meal at the temperature of 50 ℃ and the feed-liquid ratio of 1:25, pH10.5 (0.008 mol/L), alkali extraction under the condition of 3.5h, centrifugation to obtain alkali extraction supernatant, acid precipitation under the condition of pH4.0 and 2h, centrifugation to obtain protein water, washing to neutrality, spray drying to obtain oil peony protein isolate with protein extraction rate of 51.62% and purity of 94%.
Example 3:
step one: crushing and granulating the peony seed meal subjected to hydraulic pressure, and extracting for 3 times under the condition that the pressure is 0.6mpa and the extraction temperature is 45 ℃ by taking butane as an extracting agent to obtain the peony seed meal for degreasing oil with the oil content of 1.15%;
step two: and (3) taking methanol as a solvent for the peony seed meal for degreasing oil obtained in the step one, wherein the alcohol concentration is 80%, and the feed liquid ratio is 1:15, extracting for 1 time at the extraction temperature of 50 ℃ for 2 hours to obtain the peony seed meal for degreasing oil with the alcohol extract removed, wherein the yield of the alcohol extract is 12%;
step three: the degreasing oil of the ethanol extract obtained in the second step is subjected to peony seed meal at the temperature of 40 ℃ and the feed-liquid ratio of 1:25, pH10.5 (0.008 mol/L), and centrifuging for 4h to obtain an alkali extract supernatant, and acid precipitating the alkali extract supernatant at pH5 and 3h, centrifuging, washing the obtained protein with water to neutrality, and spray drying to obtain oil peony protein isolate with protein extraction rate of 47.9% and purity of 93%.
The results of functional comparison of the oil peony isolated protein prepared in example 2 with soybean isolated protein are shown in Table 1.
TABLE 1 functional comparison of peony isolated protein and soybean isolated protein for oil
As is clear from the data in Table 1, the isolated soy protein obtained in example 2 has superior functional properties such as water absorption, oil retention, emulsifying property and emulsion stability, foamability and foam stability.
The application discovers that the color of the separated protein can be improved after the alcohol extraction for the first time, and meanwhile, the alcohol extract contains flavone, paeoniflorin and other components and has high recycling value, so the application analyzes how the alcohol extract can be effectively extracted with high efficiency, and the analysis process is shown in tables 2 and 3.
TABLE 2 response surface optimization test results for treatment of peony seed meal with methanol (yield of ethanol extract based on rutin content)
TABLE 3 response surface optimization results of ethanol treatment of peony seed meal (ethanol extract yield based on rutin content)
As can be seen from the data in tables 2 and 3, the feed/liquid ratio 1:5-1:15, the extraction temperature is 40-50 ℃ and the extraction time is 1-3 h, the alcohol extract containing the flavone can be extracted efficiently, thereby ensuring the quality of the separated protein and facilitating the subsequent acquisition and reutilization of the alcohol extract containing the flavone.
The process of the application for extracting protein with alkali is also optimized, and the process is shown in table 4.
Table 4 orthogonal test optimized alkaline extracted protein extraction protocol and results
As can be seen from Table 4, the temperature is 40-50 ℃, the time is 2.5-3.5h, the pH is controlled between 10-11, and the feed-liquid ratio is 1:15-1:25, can obtain the separated protein with higher purity and yield.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present application, and are not limiting; although the application has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present application.
Claims (1)
1. A method for extracting peony isolated protein for oil is characterized by comprising the following steps: the method comprises the following steps:
step one: the method comprises the steps of (1) removing shell from oil peony seeds, pressing cakes, crushing, granulating, extracting for 1-4 times at the extraction temperature of 20-50 ℃ under the pressure of 0.3-0.8 mpa by using butane as an extracting agent to obtain defatted oil peony seed meal with the oil content of less than or equal to 1.5%;
step two: and (3) taking methanol or ethanol as a solvent for the peony seed meal for the degreasing oil obtained in the step one, wherein the alcohol concentration is 50% -95%, and the feed liquid ratio is 1:5-1:15, extracting for 1-3 times at the extraction temperature of 40-50 ℃ for 1-3 hours to obtain the peony seed meal for degreasing oil with the alcohol extract removed;
step three: the defatted oil obtained in the second step and the alcohol extract removed is subjected to peony seed meal at the temperature of 30-50 ℃ and the feed-liquid ratio of 1:15-1:25, carrying out alkaline extraction under the conditions of pH of 10-11 and time of 2-5 h, centrifuging to obtain alkaline extraction supernatant, carrying out acid precipitation on the alkaline extraction supernatant under the conditions of pH of 3-5 and time of 1-4 h, centrifuging, washing the obtained protein with water to be neutral, and spray drying to obtain the peony protein isolate for oil.
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